MD03G1091800.v1.1

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005929
Physical Location & Seq
Forward (+)
7626805 .. 7627522
718 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1091800.v1.1.491

Sequence Viewer

Length: 132 bp
ATGGGGATCAAGGCATTACGTGTTGTTGATGGATCTGTATTCAATTTATCACCAGGGACCAATCCTCAAGCCACTCTCATGATGTTGGGCAGGTATGTTGGCGTTCAAATGCTGGAAGAAAGATCAGAGTGA

Protein Analysis

44

Amino Acids

4.7

Weight (kDa)

6.11

Isoelectric Point (pI)

33.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_C PF05199 1 - 30 1.2e-06 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 81
AclWI GGATC 2 cut(s) 14, 40
AflIII ACRYGT 1 cut(s) 19
AgsI TTSAA 2 cut(s) 43, 107
AjnI CCWGG 1 cut(s) 52
AlwI GGATC 2 cut(s) 14, 40
AspS9I GGNCC 1 cut(s) 57
AsuHPI GGTGA 1 cut(s) 42
AvaII GGWCC 1 cut(s) 57
BccI CCATC 1 cut(s) 23
BciT130I CCWGG 1 cut(s) 54
BfuAI ACCTGC 1 cut(s) 81
Bme1390I CCNGG 1 cut(s) 54
Bme18I GGWCC 1 cut(s) 57
BmgT120I GGNCC 1 cut(s) 57
BmiI GGNNCC 1 cut(s) 58
BmrFI CCNGG 1 cut(s) 54
BpuEI CTTGAG 1 cut(s) 51
BsaAI YACGTR 1 cut(s) 20
BsaJI CCNNGG 1 cut(s) 53
BseBI CCWGG 1 cut(s) 54
BseDI CCNNGG 1 cut(s) 53
BslFI GGGAC 1 cut(s) 70
BsmFI GGGAC 1 cut(s) 70
Bsp143I GATC 3 cut(s) 6, 32, 122
BspHI TCATGA 1 cut(s) 78
BspLI GGNNCC 1 cut(s) 58
BspMI ACCTGC 1 cut(s) 81
BspPI GGATC 2 cut(s) 14, 40
BssECI CCNNGG 1 cut(s) 53
BssMI GATC 3 cut(s) 6, 32, 122
Bst2UI CCWGG 1 cut(s) 54
BstBAI YACGTR 1 cut(s) 20
BstKTI GATC 3 cut(s) 9, 35, 125
BstMBI GATC 3 cut(s) 6, 32, 122
BstNI CCWGG 1 cut(s) 54
BstSCI CCNGG 1 cut(s) 52
BstX2I RGATCY 1 cut(s) 32
BstYI RGATCY 1 cut(s) 32
BveI ACCTGC 1 cut(s) 81
CciI TCATGA 1 cut(s) 78
Cfr13I GGNCC 1 cut(s) 57
CviAII CATG 1 cut(s) 79
CviJI RGCY 1 cut(s) 71
CviKI_1 RGCY 1 cut(s) 71
DpnI GATC 3 cut(s) 8, 34, 124
DpnII GATC 3 cut(s) 6, 32, 122
Eco47I GGWCC 1 cut(s) 57
EcoRII CCWGG 1 cut(s) 52
FaeI CATG 1 cut(s) 82
FaiI YATR 2 cut(s) 80, 96
FaqI GGGAC 1 cut(s) 70
FatI CATG 1 cut(s) 78
Hin1II CATG 1 cut(s) 82
HphI GGTGA 1 cut(s) 42
Hpy188I TCNGA 1 cut(s) 127
Hpy188III TCNNGA 1 cut(s) 79
HpyCH4IV ACGT 1 cut(s) 19
HpySE526I ACGT 1 cut(s) 19
Hsp92II CATG 1 cut(s) 82
Kzo9I GATC 3 cut(s) 6, 32, 122
LpnPI CCDG 4 cut(s) 39, 66, 76, 98
MaeII ACGT 1 cut(s) 19
MalI GATC 3 cut(s) 8, 34, 124
MboI GATC 3 cut(s) 6, 32, 122
MboII GAAGA 1 cut(s) 128
MflI RGATCY 1 cut(s) 32
MluCI AATT 1 cut(s) 43
MnlI CCTC 1 cut(s) 75
MslI CAYNNNNRTG 1 cut(s) 77
MspR9I CCNGG 1 cut(s) 54
MvaI CCWGG 1 cut(s) 54
NdeII GATC 3 cut(s) 6, 32, 122
NlaIII CATG 1 cut(s) 82
NlaIV GGNNCC 1 cut(s) 58
PagI TCATGA 1 cut(s) 78
Ppu21I YACGTR 1 cut(s) 20
Psp6I CCWGG 1 cut(s) 52
PspGI CCWGG 1 cut(s) 52
PspN4I GGNNCC 1 cut(s) 58
PspPI GGNCC 1 cut(s) 57
PsuI RGATCY 1 cut(s) 32
RseI CAYNNNNRTG 1 cut(s) 77
Sau3AI GATC 3 cut(s) 6, 32, 122
Sau96I GGNCC 1 cut(s) 57
ScrFI CCNGG 1 cut(s) 54
SetI ASST 2 cut(s) 22, 95
SgeI CNNG 8 cut(s) 22, 32, 65, 66, 80, 91, 103, 125
SinI GGWCC 1 cut(s) 57
SmiMI CAYNNNNRTG 1 cut(s) 77
SmlI CTYRAG 1 cut(s) 66
SmoI CTYRAG 1 cut(s) 66
Sse9I AATT 1 cut(s) 43
StyD4I CCNGG 1 cut(s) 52
TaiI ACGT 1 cut(s) 22
TasI AATT 1 cut(s) 43
VpaK11BI GGWCC 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.