Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0004871

Physical Location & Seq

Genomic Coordinates Reverse (-)

26497882 .. 26500801

Gene Structure 2920 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1123600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 834 bp

ATGCTCGCTCTCCCTTCTTCTCGTCTTGTTCCTTCTCACCACTTTCCCAAAACCCATATCCAGAAAACTCACACACGCAACCCCACTAAATCCGGATCTACCTCACTAACAATGGCCTCCCTTTCATCAAATGCTCACCCGCTCGATAGAAAATCTTCTCTAGCTTGTGTCGCACCTCTAGAAGCAATTCTATTTGATATTGATGGAACATTGTGTGATTCAGATCCCTTCCATTATTATGCTTTCCGTGAAATGCTTCAAGAAGTAGGTTTTAATGGTGGGGTTCCTATCACTGAGGAATTCTTCAGTGAGAAGATAGCTGGAGTTCATAATGAGTATCTTTGTAGTATCCTCTTTCCTGATTGGGATATCGAAAGAGCAAGACAGTTTTTGGTAGATAAGGAAGCCATGTTCCGAAGATTTATATCTGAAAAGATAGAACCTCTTAAGGGCCTCCCTAGGTTGCGCCAATGGATTGAAAAACCAGGCTTCAGAAGGGCTGCAGTAACAAATGCTACAAAACTAAATGCCGAGCTAATATTATCAAAGTTGAAGCTCACGGATTTCTTTGAAATTGTTGTTCTTGGAGAGGAATGCATACGAGCAAAACCATTTCCTGATCCTTACTTGACGGCTCTTGAAAAGCTTAACCTGTCAAACGAGCATGCTTTCATCTTTGAGGCTGGAGTAGCAGCCGGAATGCCAGTAGTGGGTTTAGGCACAAGGAACCCCGAGGAAGCATTGATTAATGCGGGAGCCTCCTTTGTTATTAAAGATTTTGATGACCCAAAATTGTGGGAAGCACTGGAAGAGATTGTGAAGAAGGTGGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HAD_2

Protein Analysis

278

Amino Acids

31.12

Weight (kDa)

5.82

Isoelectric Point (pI)

48.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hydrolase	PF00702	61 - 231	2.9e-18	haloacid dehalogenase-like hydrolase
HAD_2	PF13419	63 - 226	8.5e-21	Haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004871)

Species	Orthologous Gene IDs
malus_domestica	MD00G1123600.v1.1 MD00G1190500.v1.1 MD02G1226100.v1.1 MD02G1226200.v1.1 MD02G1226400.v1.1 MD07G1087800.v1.1
prunus_persica	Prupe.2G111900_v2.0.a1 Prupe.2G114400_v2.0.a1
pyrus_communis	pycom02g19470 pycom02g19500
rosa_chinensis	RchiOBHm_Chr1g0343701 RchiOBHm_Chr1g0343751 RchiOBHm_Chr1g0343881 RchiOBHm_Chr1g0360261
rosa_laevigata	RLG00000028950 RLG00000028952
rosa_multiflora	Rmu_sc0004270.1_g000007 Rmu_sc0007032.1_g000015 Rmu_ssc0000259.1_g000047
rosa_roxburghii	Rroxscaffold_4G00310310
rosa_rugosa	Rorug01G0168900 Rorug01G0169000
rosa_samantha	Rh1AG185100 Rh1AG185500 Rh1BG125700 Rh1BG152400 Rh1CG171300 Rh1DG184400 Rh6BG211900
rosa_wichuraiana	Rw1G015310 Rw1G015350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	142
AccIII	TCCGGA	1 cut(s)	92
AciI	CCGC	2 cut(s)	140, 752
AclWI	GGATC	3 cut(s)	103, 218, 614
AcsI	RAATTY	1 cut(s)	299
AcuI	CTGAAG	2 cut(s)	289, 475
AfiI	CCNNNNNNNGG	2 cut(s)	449, 710
AflII	CTTAAG	1 cut(s)	446
AgsI	TTSAA	5 cut(s)	260, 479, 553, 572, 641
AjnI	CCWGG	1 cut(s)	484
AloI	GAACNNNNNNTCC	2 cut(s)	395, 427
AluBI	AGCT	5 cut(s)	164, 320, 535, 556, 646
AluI	AGCT	5 cut(s)	164, 320, 535, 556, 646
AlwI	GGATC	3 cut(s)	103, 218, 614
Ama87I	CYCGRG	1 cut(s)	731
Aor13HI	TCCGGA	1 cut(s)	92
AoxI	GGCC	2 cut(s)	114, 451
ApeKI	GCWGC	2 cut(s)	500, 692
ApoI	RAATTY	1 cut(s)	299
AseI	ATTAAT	1 cut(s)	747
Asp700I	GAANNNNTTC	3 cut(s)	154, 186, 255
AspA2I	CCTAGG	1 cut(s)	458
AspLEI	GCGC	1 cut(s)	468
AspS9I	GGNCC	1 cut(s)	451
AsuHPI	GGTGA	2 cut(s)	29, 128
AvaI	CYCGRG	1 cut(s)	731
AvrII	CCTAGG	1 cut(s)	458
BbvI	GCAGC	2 cut(s)	487, 704
BccI	CCATC	1 cut(s)	197
BceAI	ACGGC	1 cut(s)	648
BciT130I	CCWGG	1 cut(s)	486
BciVI	GTATCC	1 cut(s)	359
BfaI	CTAG	3 cut(s)	161, 179, 459
BfmI	CTRYAG	1 cut(s)	501
BfrI	CTTAAG	1 cut(s)	446
BfuI	GTATCC	1 cut(s)	359
BisI	GCNGC	2 cut(s)	501, 693
BlnI	CCTAGG	1 cut(s)	458
BlsI	GCNGC	2 cut(s)	502, 694
Bme1390I	CCNGG	1 cut(s)	486
BmeT110I	CYCGRG	1 cut(s)	731
BmgT120I	GGNCC	1 cut(s)	451
BmiI	GGNNCC	3 cut(s)	285, 728, 757
BmrFI	CCNGG	1 cut(s)	486
BpmI	CTGGAG	2 cut(s)	342, 705
BsaBI	GATNNNNATC	2 cut(s)	222, 424
BsaJI	CCNNGG	2 cut(s)	458, 732
BsaWI	WCCGGW	1 cut(s)	92
Bsc4I	CCNNNNNNNGG	2 cut(s)	449, 710
Bse1I	ACTGG	2 cut(s)	704, 810
Bse8I	GATNNNNATC	2 cut(s)	222, 424
BseAI	TCCGGA	1 cut(s)	92
BseBI	CCWGG	1 cut(s)	486
BseDI	CCNNGG	2 cut(s)	458, 732
BseJI	GATNNNNATC	2 cut(s)	222, 424
BseLI	CCNNNNNNNGG	2 cut(s)	449, 710
BseMII	CTCAG	1 cut(s)	285
BseNI	ACTGG	2 cut(s)	704, 810
BseXI	GCAGC	2 cut(s)	487, 704
BshFI	GGCC	2 cut(s)	116, 453
BsiHKCI	CYCGRG	1 cut(s)	731
BsiSI	CCGG	2 cut(s)	93, 696
BslI	CCNNNNNNNGG	2 cut(s)	449, 710
BsmI	GAATGC	2 cut(s)	599, 705
BsnI	GGCC	2 cut(s)	116, 453
BsoBI	CYCGRG	1 cut(s)	731
Bsp13I	TCCGGA	1 cut(s)	92
Bsp143I	GATC	3 cut(s)	95, 223, 619
BspACI	CCGC	2 cut(s)	140, 752
BspANI	GGCC	2 cut(s)	116, 453
BspCNI	CTCAG	1 cut(s)	286
BspEI	TCCGGA	1 cut(s)	92
BspLI	GGNNCC	3 cut(s)	285, 728, 757
BspMAI	CTGCAG	1 cut(s)	505
BspPI	GGATC	3 cut(s)	103, 218, 614
BspTI	CTTAAG	1 cut(s)	446
BsrBI	CCGCTC	1 cut(s)	142
BsrI	ACTGG	2 cut(s)	704, 810
BssECI	CCNNGG	2 cut(s)	458, 732
BssMI	GATC	3 cut(s)	95, 223, 619
BssT1I	CCWWGG	1 cut(s)	458
Bst2UI	CCWGG	1 cut(s)	486
Bst4CI	ACNGT	1 cut(s)	387
Bst6I	CTCTTC	1 cut(s)	804
BstAFI	CTTAAG	1 cut(s)	446
BstC8I	GCNNGC	2 cut(s)	6, 666
BstDEI	CTNAG	1 cut(s)	294
BstHHI	GCGC	1 cut(s)	468
BstKTI	GATC	3 cut(s)	98, 226, 622
BstMBI	GATC	3 cut(s)	95, 223, 619
BstMWI	GCNNNNNNNGC	2 cut(s)	170, 689
BstNI	CCWGG	1 cut(s)	486
BstNSI	RCATGY	1 cut(s)	668
BstSCI	CCNGG	1 cut(s)	484
BstSFI	CTRYAG	1 cut(s)	501
BstV1I	GCAGC	2 cut(s)	487, 704
BstX2I	RGATCY	2 cut(s)	95, 223
BstXI	CCANNNNNNTGG	1 cut(s)	795
BstYI	RGATCY	2 cut(s)	95, 223
BsuI	GTATCC	1 cut(s)	359
BsuRI	GGCC	2 cut(s)	116, 453
BtsIMutI	CAGTG	3 cut(s)	291, 313, 803
Cac8I	GCNNGC	2 cut(s)	6, 666
CfoI	GCGC	1 cut(s)	468
Cfr13I	GGNCC	1 cut(s)	451
CviAII	CATG	2 cut(s)	409, 665
DdeI	CTNAG	1 cut(s)	294
DpnI	GATC	3 cut(s)	97, 225, 621
DpnII	GATC	3 cut(s)	95, 223, 619
Eam1104I	CTCTTC	1 cut(s)	804
EarI	CTCTTC	1 cut(s)	804
Eco130I	CCWWGG	1 cut(s)	458
Eco32I	GATATC	1 cut(s)	370
Eco57I	CTGAAG	2 cut(s)	289, 475
Eco88I	CYCGRG	1 cut(s)	731
EcoO109I	RGGNCCY	1 cut(s)	451
EcoRI	GAATTC	1 cut(s)	299
EcoRII	CCWGG	1 cut(s)	484
EcoRV	GATATC	1 cut(s)	370
EcoT14I	CCWWGG	1 cut(s)	458
EcoT22I	ATGCAT	1 cut(s)	599
ErhI	CCWWGG	1 cut(s)	458
FaeI	CATG	2 cut(s)	412, 668
FaiI	YATR	7 cut(s)	57, 240, 330, 410, 425, 599, 666
FatI	CATG	2 cut(s)	408, 664
FauI	CCCGC	2 cut(s)	147, 745
Fnu4HI	GCNGC	2 cut(s)	501, 693
Fsp4HI	GCNGC	2 cut(s)	501, 693
FspBI	CTAG	3 cut(s)	161, 179, 459
GlaI	GCGC	1 cut(s)	467
GluI	GCNGC	2 cut(s)	501, 693
GsuI	CTGGAG	2 cut(s)	342, 705
HaeIII	GGCC	2 cut(s)	116, 453
HapII	CCGG	2 cut(s)	93, 696
HhaI	GCGC	1 cut(s)	468
Hin1II	CATG	2 cut(s)	412, 668
Hin6I	GCGC	1 cut(s)	466
HinP1I	GCGC	1 cut(s)	466
HindIII	AAGCTT	1 cut(s)	644
HinfI	GANTC	1 cut(s)	218
HpaII	CCGG	2 cut(s)	93, 696
HphI	GGTGA	2 cut(s)	29, 128
Hpy188I	TCNGA	4 cut(s)	223, 416, 430, 494
Hpy188III	TCNNGA	7 cut(s)	61, 93, 179, 260, 359, 617, 638
HpyAV	CCTTC	5 cut(s)	24, 42, 238, 489, 817
HpyCH4III	ACNGT	1 cut(s)	387
HpyCH4V	TGCA	2 cut(s)	503, 597
HpyF10VI	GCNNNNNNNGC	2 cut(s)	170, 689
HpyF3I	CTNAG	1 cut(s)	294
Hsp92II	CATG	2 cut(s)	412, 668
HspAI	GCGC	1 cut(s)	466
Kpn2I	TCCGGA	1 cut(s)	92
Kzo9I	GATC	3 cut(s)	95, 223, 619
LmnI	GCTCC	1 cut(s)	755
Lsp1109I	GCAGC	2 cut(s)	487, 704
MaeI	CTAG	3 cut(s)	161, 179, 459
MaeIII	GTNAC	1 cut(s)	505
MalI	GATC	3 cut(s)	97, 225, 621
MbiI	CCGCTC	1 cut(s)	142
MboI	GATC	3 cut(s)	95, 223, 619
MboII	GAAGA	7 cut(s)	9, 147, 295, 325, 429, 821, 832
MflI	RGATCY	2 cut(s)	95, 223
MluCI	AATT	4 cut(s)	186, 299, 573, 791
Mph1103I	ATGCAT	1 cut(s)	599
MroI	TCCGGA	1 cut(s)	92
MroXI	GAANNNNTTC	3 cut(s)	154, 186, 255
MseI	TTAA	5 cut(s)	273, 447, 648, 747, 771
MslI	CAYNNNNRTG	1 cut(s)	237
MspCI	CTTAAG	1 cut(s)	446
MspI	CCGG	2 cut(s)	93, 696
MspR9I	CCNGG	1 cut(s)	486
Mva1269I	GAATGC	2 cut(s)	599, 705
MvaI	CCWGG	1 cut(s)	486
MwoI	GCNNNNNNNGC	2 cut(s)	170, 689
NdeII	GATC	3 cut(s)	95, 223, 619
NlaIII	CATG	2 cut(s)	412, 668
NlaIV	GGNNCC	3 cut(s)	285, 728, 757
NmeAIII	GCCGAG	1 cut(s)	556
NsiI	ATGCAT	1 cut(s)	599
NspI	RCATGY	1 cut(s)	668
PaeI	GCATGC	1 cut(s)	668
PctI	GAATGC	2 cut(s)	599, 705
PdmI	GAANNNNTTC	3 cut(s)	154, 186, 255
PfeI	GAWTC	1 cut(s)	218
PkrI	GCNGC	2 cut(s)	502, 694
PshBI	ATTAAT	1 cut(s)	747
Psp6I	CCWGG	1 cut(s)	484
PspGI	CCWGG	1 cut(s)	484
PspN4I	GGNNCC	3 cut(s)	285, 728, 757
PspPI	GGNCC	1 cut(s)	451
PstI	CTGCAG	1 cut(s)	505
PsuI	RGATCY	2 cut(s)	95, 223
RseI	CAYNNNNRTG	1 cut(s)	237
SaqAI	TTAA	5 cut(s)	273, 447, 648, 747, 771
SatI	GCNGC	2 cut(s)	501, 693
Sau3AI	GATC	3 cut(s)	95, 223, 619
Sau96I	GGNCC	1 cut(s)	451
ScrFI	CCNGG	1 cut(s)	486
SfcI	CTRYAG	1 cut(s)	501
SmiMI	CAYNNNNRTG	1 cut(s)	237
SmlI	CTYRAG	1 cut(s)	446
SmoI	CTYRAG	1 cut(s)	446
SphI	GCATGC	1 cut(s)	668
Sse9I	AATT	4 cut(s)	186, 299, 573, 791
SsiI	CCGC	2 cut(s)	140, 752
SspI	AATATT	1 cut(s)	540
SspMI	CTAG	3 cut(s)	161, 179, 459
StyD4I	CCNGG	1 cut(s)	484
StyI	CCWWGG	1 cut(s)	458
TaaI	ACNGT	1 cut(s)	387
TaqI	TCGA	2 cut(s)	144, 372
TasI	AATT	4 cut(s)	186, 299, 573, 791
TfiI	GAWTC	1 cut(s)	218
Tru1I	TTAA	5 cut(s)	273, 447, 648, 747, 771
Tru9I	TTAA	5 cut(s)	273, 447, 648, 747, 771
TscAI	CASTG	3 cut(s)	298, 313, 810
TseI	GCWGC	2 cut(s)	500, 692
TspDTI	ATGAA	3 cut(s)	114, 317, 661
TspGWI	ACGGA	2 cut(s)	236, 575
TspRI	CASTG	3 cut(s)	298, 313, 810
Vha464I	CTTAAG	1 cut(s)	446
VspI	ATTAAT	1 cut(s)	747
XapI	RAATTY	1 cut(s)	299
XbaI	TCTAGA	1 cut(s)	178
XceI	RCATGY	1 cut(s)	668
XmaJI	CCTAGG	1 cut(s)	458
XmnI	GAANNNNTTC	3 cut(s)	154, 186, 255
XspI	CTAG	3 cut(s)	161, 179, 459
Zsp2I	ATGCAT	1 cut(s)	599

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.