Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0004871

Physical Location & Seq

Genomic Coordinates Forward (+)

26540489 .. 26543771

Gene Structure 3283 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1226100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 855 bp

ATGCTCGCTCTCCCTTCTTCTCGTCTTGTTCCTTCTCACCACTTTCCCAAAACCCATCTCCAGAAAACTCACACACGCAACCCCACTAAATCCGGATCTACCTCACTAACAATGGCGTCCCTTACATCAAATGCTCACCCACTCGATAGAAAATCTTCTCTAGCTTGTGTCGCACCTCTAGAAGCAATTCTATTTGATATTGATGGAACATTGTGTGATTCAGATCCCTTCCATTATTATGCTTTCCGTGAAATGCTTCAAGAAGTAGGTTTTAATGGTGGGGTTCCTATCACTGAGGAATTCTACAGTGAGAAGATAACTGGAGGTCATAATGAGTATCTTTGTAGTATCCTCTTTCCTGATTGGGATATCGAAAGAGCAAGACAGTTTTTGGTAGATAAGGAAGCCATGTTCCGAAGATTGATATCTGAAAAGATAGAACCCCTTAAGGGCCTACCTAGGTTGCGCCAATGGATTGAAAAACAAGGCTTGAGACGGGCTGCAGTAACAAATGCTCCAAAACTAAATGCCGAGCTAATATTATCAAAGTTGAAGCTCACGGATTTCTTTGAAATTGTTGTTCTTGGAGAGGAATGCATACGAGCAAAACCATTTCCTGATCCTTACTTGACGGCTCTTGAAAAGCTTAACCTGTCAAACGAGCATGCCTTCATCTTTGAGGATTCTGTTTCGGGAGTGAAGGCTGGAGTAGCAGCCGGAATGCCGGTAGTGGGTTTAGGCACAAGGAACCCCGAGGAAGCATTGATTAATGCGGGAGCCTCCTTTGTTATTAAAGATTTTGATGACCCAAAATTGTGGGAAGCACTGGAAGAGATTGTGAAGAAGGTGGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HAD_2

Protein Analysis

285

Amino Acids

31.77

Weight (kDa)

5.82

Isoelectric Point (pI)

51.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hydrolase	PF00702	61 - 240	3.5e-24	haloacid dehalogenase-like hydrolase
HAD_2	PF13419	63 - 245	8.7e-27	Haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004871)

Species	Orthologous Gene IDs
malus_domestica	MD00G1123600.v1.1 MD00G1190500.v1.1 MD02G1226100.v1.1 MD02G1226200.v1.1 MD02G1226400.v1.1 MD07G1087800.v1.1
prunus_persica	Prupe.2G111900_v2.0.a1 Prupe.2G114400_v2.0.a1
pyrus_communis	pycom02g19470 pycom02g19500
rosa_chinensis	RchiOBHm_Chr1g0343701 RchiOBHm_Chr1g0343751 RchiOBHm_Chr1g0343881 RchiOBHm_Chr1g0360261
rosa_laevigata	RLG00000028950 RLG00000028952
rosa_multiflora	Rmu_sc0004270.1_g000007 Rmu_sc0007032.1_g000015 Rmu_ssc0000259.1_g000047
rosa_roxburghii	Rroxscaffold_4G00310310
rosa_rugosa	Rorug01G0168900 Rorug01G0169000
rosa_samantha	Rh1AG185100 Rh1AG185500 Rh1BG125700 Rh1BG152400 Rh1CG171300 Rh1DG184400 Rh6BG211900
rosa_wichuraiana	Rw1G015310 Rw1G015350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccIII	TCCGGA	1 cut(s)	92
AciI	CCGC	1 cut(s)	773
AclWI	GGATC	3 cut(s)	103, 218, 614
AcsI	RAATTY	1 cut(s)	299
AcyI	GRCGYC	1 cut(s)	116
AfiI	CCNNNNNNNGG	2 cut(s)	449, 731
AflII	CTTAAG	1 cut(s)	446
AgsI	TTSAA	5 cut(s)	260, 479, 553, 572, 641
AloI	GAACNNNNNNTCC	2 cut(s)	395, 427
AluBI	AGCT	4 cut(s)	164, 535, 556, 646
AluI	AGCT	4 cut(s)	164, 535, 556, 646
Alw26I	GTCTC	1 cut(s)	487
AlwI	GGATC	3 cut(s)	103, 218, 614
Ama87I	CYCGRG	1 cut(s)	752
Aor13HI	TCCGGA	1 cut(s)	92
AoxI	GGCC	1 cut(s)	451
ApeKI	GCWGC	2 cut(s)	500, 713
ApoI	RAATTY	1 cut(s)	299
AseI	ATTAAT	1 cut(s)	768
Asp700I	GAANNNNTTC	3 cut(s)	154, 186, 255
AspA2I	CCTAGG	1 cut(s)	458
AspLEI	GCGC	1 cut(s)	468
AspS9I	GGNCC	1 cut(s)	451
AsuHPI	GGTGA	2 cut(s)	29, 128
AvaI	CYCGRG	1 cut(s)	752
AvrII	CCTAGG	1 cut(s)	458
BbvI	GCAGC	2 cut(s)	487, 725
BccI	CCATC	2 cut(s)	63, 197
BceAI	ACGGC	1 cut(s)	648
BciVI	GTATCC	1 cut(s)	359
BcoDI	GTCTC	1 cut(s)	487
BfaI	CTAG	3 cut(s)	161, 179, 459
BfmI	CTRYAG	2 cut(s)	304, 501
BfrI	CTTAAG	1 cut(s)	446
BfuI	GTATCC	1 cut(s)	359
BisI	GCNGC	2 cut(s)	501, 714
BlnI	CCTAGG	1 cut(s)	458
BlsI	GCNGC	2 cut(s)	502, 715
BmeT110I	CYCGRG	1 cut(s)	752
BmgT120I	GGNCC	1 cut(s)	451
BmiI	GGNNCC	3 cut(s)	285, 749, 778
BpmI	CTGGAG	3 cut(s)	44, 342, 726
BpuEI	CTTGAG	1 cut(s)	511
BsaBI	GATNNNNATC	2 cut(s)	222, 424
BsaHI	GRCGYC	1 cut(s)	116
BsaJI	CCNNGG	2 cut(s)	458, 753
BsaWI	WCCGGW	1 cut(s)	92
BsaXI	ACNNNNNCTCC	2 cut(s)	499, 529
Bsc4I	CCNNNNNNNGG	2 cut(s)	449, 731
Bse118I	RCCGGY	1 cut(s)	724
Bse1I	ACTGG	2 cut(s)	325, 831
Bse8I	GATNNNNATC	2 cut(s)	222, 424
BseAI	TCCGGA	1 cut(s)	92
BseDI	CCNNGG	2 cut(s)	458, 753
BseJI	GATNNNNATC	2 cut(s)	222, 424
BseLI	CCNNNNNNNGG	2 cut(s)	449, 731
BseMII	CTCAG	1 cut(s)	285
BseNI	ACTGG	2 cut(s)	325, 831
BseXI	GCAGC	2 cut(s)	487, 725
BshFI	GGCC	1 cut(s)	453
BsiHKCI	CYCGRG	1 cut(s)	752
BsiSI	CCGG	3 cut(s)	93, 717, 725
BslFI	GGGAC	1 cut(s)	103
BslI	CCNNNNNNNGG	2 cut(s)	449, 731
BsmAI	GTCTC	1 cut(s)	487
BsmBI	CGTCTC	1 cut(s)	487
BsmFI	GGGAC	1 cut(s)	103
BsmI	GAATGC	2 cut(s)	599, 726
BsnI	GGCC	1 cut(s)	453
BsoBI	CYCGRG	1 cut(s)	752
Bsp13I	TCCGGA	1 cut(s)	92
Bsp143I	GATC	3 cut(s)	95, 223, 619
BspACI	CCGC	1 cut(s)	773
BspANI	GGCC	1 cut(s)	453
BspCNI	CTCAG	1 cut(s)	286
BspEI	TCCGGA	1 cut(s)	92
BspLI	GGNNCC	3 cut(s)	285, 749, 778
BspMAI	CTGCAG	1 cut(s)	505
BspPI	GGATC	3 cut(s)	103, 218, 614
BspTI	CTTAAG	1 cut(s)	446
BsrFI	RCCGGY	1 cut(s)	724
BsrI	ACTGG	2 cut(s)	325, 831
BssAI	RCCGGY	1 cut(s)	724
BssECI	CCNNGG	2 cut(s)	458, 753
BssMI	GATC	3 cut(s)	95, 223, 619
BssNI	GRCGYC	1 cut(s)	116
BssT1I	CCWWGG	1 cut(s)	458
Bst4CI	ACNGT	2 cut(s)	308, 387
Bst6I	CTCTTC	1 cut(s)	825
BstACI	GRCGYC	1 cut(s)	116
BstAFI	CTTAAG	1 cut(s)	446
BstC8I	GCNNGC	2 cut(s)	6, 666
BstDEI	CTNAG	1 cut(s)	294
BstHHI	GCGC	1 cut(s)	468
BstKTI	GATC	3 cut(s)	98, 226, 622
BstMAI	GTCTC	1 cut(s)	487
BstMBI	GATC	3 cut(s)	95, 223, 619
BstMWI	GCNNNNNNNGC	2 cut(s)	170, 710
BstNSI	RCATGY	1 cut(s)	668
BstSFI	CTRYAG	2 cut(s)	304, 501
BstV1I	GCAGC	2 cut(s)	487, 725
BstX2I	RGATCY	2 cut(s)	95, 223
BstXI	CCANNNNNNTGG	1 cut(s)	816
BstYI	RGATCY	2 cut(s)	95, 223
BsuI	GTATCC	1 cut(s)	359
BsuRI	GGCC	1 cut(s)	453
BtsIMutI	CAGTG	3 cut(s)	291, 313, 824
Cac8I	GCNNGC	2 cut(s)	6, 666
CfoI	GCGC	1 cut(s)	468
Cfr10I	RCCGGY	1 cut(s)	724
Cfr13I	GGNCC	1 cut(s)	451
CseI	GACGC	1 cut(s)	105
CviAII	CATG	2 cut(s)	409, 665
DdeI	CTNAG	1 cut(s)	294
DpnI	GATC	3 cut(s)	97, 225, 621
DpnII	GATC	3 cut(s)	95, 223, 619
Eam1104I	CTCTTC	1 cut(s)	825
EarI	CTCTTC	1 cut(s)	825
Eco130I	CCWWGG	1 cut(s)	458
Eco32I	GATATC	2 cut(s)	370, 426
Eco88I	CYCGRG	1 cut(s)	752
EcoO109I	RGGNCCY	1 cut(s)	451
EcoRI	GAATTC	1 cut(s)	299
EcoRV	GATATC	2 cut(s)	370, 426
EcoT14I	CCWWGG	1 cut(s)	458
EcoT22I	ATGCAT	1 cut(s)	599
ErhI	CCWWGG	1 cut(s)	458
Esp3I	CGTCTC	1 cut(s)	487
FaeI	CATG	2 cut(s)	412, 668
FaiI	YATR	5 cut(s)	240, 330, 410, 599, 666
FaqI	GGGAC	1 cut(s)	103
FatI	CATG	2 cut(s)	408, 664
FauI	CCCGC	1 cut(s)	766
Fnu4HI	GCNGC	2 cut(s)	501, 714
Fsp4HI	GCNGC	2 cut(s)	501, 714
FspBI	CTAG	3 cut(s)	161, 179, 459
GlaI	GCGC	1 cut(s)	467
GluI	GCNGC	2 cut(s)	501, 714
GsuI	CTGGAG	3 cut(s)	44, 342, 726
HaeIII	GGCC	1 cut(s)	453
HapII	CCGG	3 cut(s)	93, 717, 725
HgaI	GACGC	1 cut(s)	105
HhaI	GCGC	1 cut(s)	468
Hin1I	GRCGYC	1 cut(s)	116
Hin1II	CATG	2 cut(s)	412, 668
Hin6I	GCGC	1 cut(s)	466
HinP1I	GCGC	1 cut(s)	466
HindIII	AAGCTT	1 cut(s)	644
HinfI	GANTC	2 cut(s)	218, 683
HpaII	CCGG	3 cut(s)	93, 717, 725
HphI	GGTGA	2 cut(s)	29, 128
Hpy188I	TCNGA	3 cut(s)	223, 416, 430
Hpy188III	TCNNGA	8 cut(s)	61, 93, 179, 260, 359, 617, 638, 693
HpyAV	CCTTC	6 cut(s)	24, 42, 238, 679, 694, 838
HpyCH4III	ACNGT	2 cut(s)	308, 387
HpyCH4V	TGCA	2 cut(s)	503, 597
HpyF10VI	GCNNNNNNNGC	2 cut(s)	170, 710
HpyF3I	CTNAG	1 cut(s)	294
Hsp92I	GRCGYC	1 cut(s)	116
Hsp92II	CATG	2 cut(s)	412, 668
HspAI	GCGC	1 cut(s)	466
Kpn2I	TCCGGA	1 cut(s)	92
Kzo9I	GATC	3 cut(s)	95, 223, 619
LmnI	GCTCC	2 cut(s)	520, 776
Lsp1109I	GCAGC	2 cut(s)	487, 725
MaeI	CTAG	3 cut(s)	161, 179, 459
MaeIII	GTNAC	1 cut(s)	505
MalI	GATC	3 cut(s)	97, 225, 621
MboI	GATC	3 cut(s)	95, 223, 619
MboII	GAAGA	6 cut(s)	9, 147, 325, 429, 842, 853
MflI	RGATCY	2 cut(s)	95, 223
MluCI	AATT	4 cut(s)	186, 299, 573, 812
MnlI	CCTC	9 cut(s)	112, 186, 289, 317, 362, 583, 673, 748, 790
Mph1103I	ATGCAT	1 cut(s)	599
MroI	TCCGGA	1 cut(s)	92
MroXI	GAANNNNTTC	3 cut(s)	154, 186, 255
MseI	TTAA	5 cut(s)	273, 447, 648, 768, 792
MslI	CAYNNNNRTG	1 cut(s)	237
MspCI	CTTAAG	1 cut(s)	446
MspI	CCGG	3 cut(s)	93, 717, 725
Mva1269I	GAATGC	2 cut(s)	599, 726
MwoI	GCNNNNNNNGC	2 cut(s)	170, 710
NdeII	GATC	3 cut(s)	95, 223, 619
NlaIII	CATG	2 cut(s)	412, 668
NlaIV	GGNNCC	3 cut(s)	285, 749, 778
NmeAIII	GCCGAG	1 cut(s)	556
NsiI	ATGCAT	1 cut(s)	599
NspI	RCATGY	1 cut(s)	668
PaeI	GCATGC	1 cut(s)	668
PctI	GAATGC	2 cut(s)	599, 726
PdmI	GAANNNNTTC	3 cut(s)	154, 186, 255
PfeI	GAWTC	2 cut(s)	218, 683
PkrI	GCNGC	2 cut(s)	502, 715
PshBI	ATTAAT	1 cut(s)	768
PspN4I	GGNNCC	3 cut(s)	285, 749, 778
PspPI	GGNCC	1 cut(s)	451
PstI	CTGCAG	1 cut(s)	505
PsuI	RGATCY	2 cut(s)	95, 223
RseI	CAYNNNNRTG	1 cut(s)	237
SaqAI	TTAA	5 cut(s)	273, 447, 648, 768, 792
SatI	GCNGC	2 cut(s)	501, 714
Sau3AI	GATC	3 cut(s)	95, 223, 619
Sau96I	GGNCC	1 cut(s)	451
SfcI	CTRYAG	2 cut(s)	304, 501
SmiMI	CAYNNNNRTG	1 cut(s)	237
SmlI	CTYRAG	2 cut(s)	446, 490
SmoI	CTYRAG	2 cut(s)	446, 490
SphI	GCATGC	1 cut(s)	668
Sse9I	AATT	4 cut(s)	186, 299, 573, 812
SsiI	CCGC	1 cut(s)	773
SspI	AATATT	1 cut(s)	540
SspMI	CTAG	3 cut(s)	161, 179, 459
StyI	CCWWGG	1 cut(s)	458
TaaI	ACNGT	2 cut(s)	308, 387
TaqI	TCGA	2 cut(s)	144, 372
TasI	AATT	4 cut(s)	186, 299, 573, 812
TfiI	GAWTC	2 cut(s)	218, 683
Tru1I	TTAA	5 cut(s)	273, 447, 648, 768, 792
Tru9I	TTAA	5 cut(s)	273, 447, 648, 768, 792
TscAI	CASTG	3 cut(s)	298, 313, 831
TseI	GCWGC	2 cut(s)	500, 713
TspDTI	ATGAA	1 cut(s)	661
TspGWI	ACGGA	2 cut(s)	236, 575
TspRI	CASTG	3 cut(s)	298, 313, 831
Vha464I	CTTAAG	1 cut(s)	446
VspI	ATTAAT	1 cut(s)	768
XapI	RAATTY	1 cut(s)	299
XbaI	TCTAGA	1 cut(s)	178
XceI	RCATGY	1 cut(s)	668
XmaJI	CCTAGG	1 cut(s)	458
XmnI	GAANNNNTTC	3 cut(s)	154, 186, 255
XspI	CTAG	3 cut(s)	161, 179, 459
Zsp2I	ATGCAT	1 cut(s)	599

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.