MD07G1087800.v1.1

Haloacid dehalogenase-like hydrolase domain-containing protein Sgpp

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004871
Physical Location & Seq
Reverse (-)
9172832 .. 9174211
1380 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1087800.v1.1.491

Sequence Viewer

Length: 327 bp
ATGCTTCAAGAAGTAGGTTTTAATGGTGGGGTTCCTATCACTGAAGCCATGTTCCGAAGATTGGTATCTGAAAAGGTAGAACCTGTTAAGGGCCTAGATAGTTTACGCCAACGGATTGAAAAACAAGGCTTGAGACGGGCTGCAGTAACAAATGCTTCAAGACTAAATGTCGAGCTACTATCATCAAAGTTGAAGCTCACAGATTTCTTTGAAATTCTTGTTCTTGGAGGGGAATGCGTTCGAGCAAAACCATTTCCTGACCCTTACTTGACGGCGCCTGAAACGCTTAAACTGTCAAATAAGCATGCTTTCATCTTTGAGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2

Protein Analysis

109

Amino Acids

12.12

Weight (kDa)

9.25

Isoelectric Point (pI)

56.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAD_2 PF13419 21 - 106 3.6e-10 Haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 274
AcsI RAATTY 1 cut(s) 213
AcuI CTGAAG 1 cut(s) 63
AcyI GRCGYC 1 cut(s) 275
AfiI CCNNNNNNNGG 2 cut(s) 61, 89
AgsI TTSAA 5 cut(s) 8, 119, 159, 193, 212
AhdI GACNNNNNGTC 1 cut(s) 167
AluBI AGCT 2 cut(s) 175, 196
AluI AGCT 2 cut(s) 175, 196
Alw26I GTCTC 1 cut(s) 127
AoxI GGCC 1 cut(s) 91
ApeKI GCWGC 1 cut(s) 140
ApoI RAATTY 1 cut(s) 213
Asp700I GAANNNNTTC 1 cut(s) 237
AspLEI GCGC 1 cut(s) 277
AspS9I GGNCC 1 cut(s) 91
BanI GGYRCC 1 cut(s) 274
BbvI GCAGC 1 cut(s) 127
BceAI ACGGC 1 cut(s) 288
BcoDI GTCTC 1 cut(s) 127
BfaI CTAG 1 cut(s) 95
BfmI CTRYAG 1 cut(s) 141
BfoI RGCGCY 1 cut(s) 278
BisI GCNGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 142
BmeRI GACNNNNNGTC 1 cut(s) 167
BmgT120I GGNCC 1 cut(s) 91
BmiI GGNNCC 2 cut(s) 33, 276
BpuEI CTTGAG 1 cut(s) 151
BsaBI GATNNNNATC 1 cut(s) 64
BsaHI GRCGYC 1 cut(s) 275
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 89
Bse8I GATNNNNATC 1 cut(s) 64
BseJI GATNNNNATC 1 cut(s) 64
BseLI CCNNNNNNNGG 2 cut(s) 61, 89
BseXI GCAGC 1 cut(s) 127
BshFI GGCC 1 cut(s) 93
BshNI GGYRCC 1 cut(s) 274
BslI CCNNNNNNNGG 2 cut(s) 61, 89
BsmAI GTCTC 1 cut(s) 127
BsmBI CGTCTC 1 cut(s) 127
BsmI GAATGC 1 cut(s) 239
BsnI GGCC 1 cut(s) 93
BspANI GGCC 1 cut(s) 93
BspLI GGNNCC 2 cut(s) 33, 276
BspMAI CTGCAG 1 cut(s) 145
BspT107I GGYRCC 1 cut(s) 274
BssNI GRCGYC 1 cut(s) 275
Bst4CI ACNGT 1 cut(s) 294
BstACI GRCGYC 1 cut(s) 275
BstC8I GCNNGC 1 cut(s) 306
BstH2I RGCGCY 1 cut(s) 278
BstHHI GCGC 1 cut(s) 277
BstMAI GTCTC 1 cut(s) 127
BstMWI GCNNNNNNNGC 1 cut(s) 283
BstNSI RCATGY 1 cut(s) 308
BstSFI CTRYAG 1 cut(s) 141
BstV1I GCAGC 1 cut(s) 127
BsuRI GGCC 1 cut(s) 93
BtsIMutI CAGTG 1 cut(s) 39
Cac8I GCNNGC 1 cut(s) 306
CfoI GCGC 1 cut(s) 277
Cfr13I GGNCC 1 cut(s) 91
CviAII CATG 2 cut(s) 49, 305
CviJI RGCY 6 cut(s) 47, 93, 129, 140, 175, 196
CviKI_1 RGCY 6 cut(s) 47, 93, 129, 140, 175, 196
DinI GGCGCC 1 cut(s) 276
DriI GACNNNNNGTC 1 cut(s) 167
Eam1105I GACNNNNNGTC 1 cut(s) 167
Eco57I CTGAAG 1 cut(s) 63
EcoO109I RGGNCCY 1 cut(s) 91
EgeI GGCGCC 1 cut(s) 276
EheI GGCGCC 1 cut(s) 276
Esp3I CGTCTC 1 cut(s) 127
FaeI CATG 2 cut(s) 52, 308
FaiI YATR 2 cut(s) 50, 306
FatI CATG 2 cut(s) 48, 304
Fnu4HI GCNGC 1 cut(s) 141
Fsp4HI GCNGC 1 cut(s) 141
FspBI CTAG 1 cut(s) 95
GlaI GCGC 1 cut(s) 276
GluI GCNGC 1 cut(s) 141
HaeII RGCGCY 1 cut(s) 278
HaeIII GGCC 1 cut(s) 93
HhaI GCGC 1 cut(s) 277
Hin1I GRCGYC 1 cut(s) 275
Hin1II CATG 2 cut(s) 52, 308
Hin6I GCGC 1 cut(s) 275
HinP1I GCGC 1 cut(s) 275
Hpy166II GTNNAC 1 cut(s) 104
Hpy188I TCNGA 2 cut(s) 56, 70
Hpy188III TCNNGA 3 cut(s) 8, 159, 257
Hpy8I GTNNAC 1 cut(s) 104
HpyCH4III ACNGT 1 cut(s) 294
HpyCH4V TGCA 1 cut(s) 143
HpyF10VI GCNNNNNNNGC 1 cut(s) 283
Hsp92I GRCGYC 1 cut(s) 275
Hsp92II CATG 2 cut(s) 52, 308
HspAI GCGC 1 cut(s) 275
KasI GGCGCC 1 cut(s) 274
LpnPI CCDG 3 cut(s) 96, 270, 291
Lsp1109I GCAGC 1 cut(s) 127
MaeI CTAG 1 cut(s) 95
MaeIII GTNAC 1 cut(s) 145
MboII GAAGA 1 cut(s) 69
MluCI AATT 1 cut(s) 213
Mly113I GGCGCC 1 cut(s) 275
MnlI CCTC 2 cut(s) 221, 313
MroXI GAANNNNTTC 1 cut(s) 237
MseI TTAA 3 cut(s) 21, 87, 288
Mva1269I GAATGC 1 cut(s) 239
MwoI GCNNNNNNNGC 1 cut(s) 283
NarI GGCGCC 1 cut(s) 275
NlaIII CATG 2 cut(s) 52, 308
NlaIV GGNNCC 2 cut(s) 33, 276
NspI RCATGY 1 cut(s) 308
PaeI GCATGC 1 cut(s) 308
PctI GAATGC 1 cut(s) 239
PdmI GAANNNNTTC 1 cut(s) 237
PkrI GCNGC 1 cut(s) 142
PluTI GGCGCC 1 cut(s) 278
PspN4I GGNNCC 2 cut(s) 33, 276
PspPI GGNCC 1 cut(s) 91
PstI CTGCAG 1 cut(s) 145
SaqAI TTAA 3 cut(s) 21, 87, 288
SatI GCNGC 1 cut(s) 141
Sau96I GGNCC 1 cut(s) 91
SetI ASST 6 cut(s) 19, 78, 85, 177, 198, 324
SfcI CTRYAG 1 cut(s) 141
SfoI GGCGCC 1 cut(s) 276
SmlI CTYRAG 1 cut(s) 130
SmoI CTYRAG 1 cut(s) 130
SphI GCATGC 1 cut(s) 308
Sse9I AATT 1 cut(s) 213
SspDI GGCGCC 1 cut(s) 274
SspMI CTAG 1 cut(s) 95
TaaI ACNGT 1 cut(s) 294
TaqI TCGA 2 cut(s) 171, 241
TasI AATT 1 cut(s) 213
Tru1I TTAA 3 cut(s) 21, 87, 288
Tru9I TTAA 3 cut(s) 21, 87, 288
TscAI CASTG 1 cut(s) 46
TseI GCWGC 1 cut(s) 140
TspDTI ATGAA 1 cut(s) 301
TspGWI ACGGA 1 cut(s) 127
TspRI CASTG 1 cut(s) 46
XapI RAATTY 1 cut(s) 213
XceI RCATGY 1 cut(s) 308
XmnI GAANNNNTTC 1 cut(s) 237
XspI CTAG 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.