Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0004871

Physical Location & Seq

Genomic Coordinates Reverse (-)

45754845 .. 45756231

Gene Structure 1387 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1190500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 603 bp

ATGCTTCAAGAAGTAGGTTTTAATGGTGGGGTTCCTATCACTGAGGAATTCTACAGTGAGAAGATAACTGGAGGTCATAATGAGTATCTTTGTAGTATCCTCTTTCCTGATTGGGATATCGAAAGAGCAAGACAGTTTTTGGTAGATAAGGAAGCCATGTTCCGAAGATTGATATCTGAAAAGATAGAACCCCTTAAGGGCCTACCTAGGTTGCGCCAATGGATTGAAAAACAAGGCTTGAGACGGGCTGCAGTAACAAATGCTCCAAAACTAAATGCCGAGCTAATATTATCAAAGTTGAAGCTCACGGATTTCTTTGAAATTGTTGTTCTTGGAGAGGAATGCATACGAGCAAAACCATTTCCTGATCCTTACTTGACGGCTCTTGAAAAGCTTAACCTGTCAAACGAGCATGCCTTCATCTTTGAGGATTCTGTTTCGGGAGTGAAGGCTGGAGTAGCAGCCGGAATGCCGGTAGTGGGTTTAGGCACAAGGAACCCCGAGGAAGCATTGATTAATGCGGGAGCCTCCTTTGTTATTAAAGATTTTGATGACCCAAAATTGTGGGAAGCACTGGAAGAGATTGTGAAGAAGGTGGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HAD_2

Protein Analysis

201

Amino Acids

22.5

Weight (kDa)

5.1

Isoelectric Point (pI)

49.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hydrolase	PF00702	20 - 156	3.4e-15	haloacid dehalogenase-like hydrolase
HAD_2	PF13419	21 - 161	1e-18	Haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004871)

Species	Orthologous Gene IDs
malus_domestica	MD00G1123600.v1.1 MD00G1190500.v1.1 MD02G1226100.v1.1 MD02G1226200.v1.1 MD02G1226400.v1.1 MD07G1087800.v1.1
prunus_persica	Prupe.2G111900_v2.0.a1 Prupe.2G114400_v2.0.a1
pyrus_communis	pycom02g19470 pycom02g19500
rosa_chinensis	RchiOBHm_Chr1g0343701 RchiOBHm_Chr1g0343751 RchiOBHm_Chr1g0343881 RchiOBHm_Chr1g0360261
rosa_laevigata	RLG00000028950 RLG00000028952
rosa_multiflora	Rmu_sc0004270.1_g000007 Rmu_sc0007032.1_g000015 Rmu_ssc0000259.1_g000047
rosa_roxburghii	Rroxscaffold_4G00310310
rosa_rugosa	Rorug01G0168900 Rorug01G0169000
rosa_samantha	Rh1AG185100 Rh1AG185500 Rh1BG125700 Rh1BG152400 Rh1CG171300 Rh1DG184400 Rh6BG211900
rosa_wichuraiana	Rw1G015310 Rw1G015350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	1 cut(s)	521
AclWI	GGATC	1 cut(s)	362
AcsI	RAATTY	1 cut(s)	47
AfiI	CCNNNNNNNGG	2 cut(s)	197, 479
AflII	CTTAAG	1 cut(s)	194
AgsI	TTSAA	5 cut(s)	8, 227, 301, 320, 389
AloI	GAACNNNNNNTCC	2 cut(s)	143, 175
AluBI	AGCT	3 cut(s)	283, 304, 394
AluI	AGCT	3 cut(s)	283, 304, 394
Alw26I	GTCTC	1 cut(s)	235
AlwI	GGATC	1 cut(s)	362
Ama87I	CYCGRG	1 cut(s)	500
AoxI	GGCC	1 cut(s)	199
ApeKI	GCWGC	2 cut(s)	248, 461
ApoI	RAATTY	1 cut(s)	47
AseI	ATTAAT	1 cut(s)	516
AspA2I	CCTAGG	1 cut(s)	206
AspLEI	GCGC	1 cut(s)	216
AspS9I	GGNCC	1 cut(s)	199
AvaI	CYCGRG	1 cut(s)	500
AvrII	CCTAGG	1 cut(s)	206
BbvI	GCAGC	2 cut(s)	235, 473
BceAI	ACGGC	1 cut(s)	396
BciVI	GTATCC	1 cut(s)	107
BcoDI	GTCTC	1 cut(s)	235
BfaI	CTAG	1 cut(s)	207
BfmI	CTRYAG	2 cut(s)	52, 249
BfrI	CTTAAG	1 cut(s)	194
BfuI	GTATCC	1 cut(s)	107
BisI	GCNGC	2 cut(s)	249, 462
BlnI	CCTAGG	1 cut(s)	206
BlsI	GCNGC	2 cut(s)	250, 463
BmeT110I	CYCGRG	1 cut(s)	500
BmgT120I	GGNCC	1 cut(s)	199
BmiI	GGNNCC	3 cut(s)	33, 497, 526
BpmI	CTGGAG	2 cut(s)	90, 474
BpuEI	CTTGAG	1 cut(s)	259
BsaBI	GATNNNNATC	1 cut(s)	172
BsaJI	CCNNGG	2 cut(s)	206, 501
BsaXI	ACNNNNNCTCC	2 cut(s)	247, 277
Bsc4I	CCNNNNNNNGG	2 cut(s)	197, 479
Bse118I	RCCGGY	1 cut(s)	472
Bse1I	ACTGG	2 cut(s)	73, 579
Bse8I	GATNNNNATC	1 cut(s)	172
BseDI	CCNNGG	2 cut(s)	206, 501
BseJI	GATNNNNATC	1 cut(s)	172
BseLI	CCNNNNNNNGG	2 cut(s)	197, 479
BseMII	CTCAG	1 cut(s)	33
BseNI	ACTGG	2 cut(s)	73, 579
BseXI	GCAGC	2 cut(s)	235, 473
BshFI	GGCC	1 cut(s)	201
BsiHKCI	CYCGRG	1 cut(s)	500
BsiSI	CCGG	2 cut(s)	465, 473
BslI	CCNNNNNNNGG	2 cut(s)	197, 479
BsmAI	GTCTC	1 cut(s)	235
BsmBI	CGTCTC	1 cut(s)	235
BsmI	GAATGC	2 cut(s)	347, 474
BsnI	GGCC	1 cut(s)	201
BsoBI	CYCGRG	1 cut(s)	500
Bsp143I	GATC	1 cut(s)	367
BspACI	CCGC	1 cut(s)	521
BspANI	GGCC	1 cut(s)	201
BspCNI	CTCAG	1 cut(s)	34
BspLI	GGNNCC	3 cut(s)	33, 497, 526
BspMAI	CTGCAG	1 cut(s)	253
BspPI	GGATC	1 cut(s)	362
BspTI	CTTAAG	1 cut(s)	194
BsrFI	RCCGGY	1 cut(s)	472
BsrI	ACTGG	2 cut(s)	73, 579
BssAI	RCCGGY	1 cut(s)	472
BssECI	CCNNGG	2 cut(s)	206, 501
BssMI	GATC	1 cut(s)	367
BssT1I	CCWWGG	1 cut(s)	206
Bst4CI	ACNGT	2 cut(s)	56, 135
Bst6I	CTCTTC	1 cut(s)	573
BstAFI	CTTAAG	1 cut(s)	194
BstC8I	GCNNGC	1 cut(s)	414
BstDEI	CTNAG	1 cut(s)	42
BstHHI	GCGC	1 cut(s)	216
BstKTI	GATC	1 cut(s)	370
BstMAI	GTCTC	1 cut(s)	235
BstMBI	GATC	1 cut(s)	367
BstMWI	GCNNNNNNNGC	1 cut(s)	458
BstNSI	RCATGY	1 cut(s)	416
BstSFI	CTRYAG	2 cut(s)	52, 249
BstV1I	GCAGC	2 cut(s)	235, 473
BstXI	CCANNNNNNTGG	1 cut(s)	564
BsuI	GTATCC	1 cut(s)	107
BsuRI	GGCC	1 cut(s)	201
BtsIMutI	CAGTG	3 cut(s)	39, 61, 572
Cac8I	GCNNGC	1 cut(s)	414
CfoI	GCGC	1 cut(s)	216
Cfr10I	RCCGGY	1 cut(s)	472
Cfr13I	GGNCC	1 cut(s)	199
CviAII	CATG	2 cut(s)	157, 413
DdeI	CTNAG	1 cut(s)	42
DpnI	GATC	1 cut(s)	369
DpnII	GATC	1 cut(s)	367
Eam1104I	CTCTTC	1 cut(s)	573
EarI	CTCTTC	1 cut(s)	573
Eco130I	CCWWGG	1 cut(s)	206
Eco32I	GATATC	2 cut(s)	118, 174
Eco88I	CYCGRG	1 cut(s)	500
EcoO109I	RGGNCCY	1 cut(s)	199
EcoRI	GAATTC	1 cut(s)	47
EcoRV	GATATC	2 cut(s)	118, 174
EcoT14I	CCWWGG	1 cut(s)	206
EcoT22I	ATGCAT	1 cut(s)	347
ErhI	CCWWGG	1 cut(s)	206
Esp3I	CGTCTC	1 cut(s)	235
FaeI	CATG	2 cut(s)	160, 416
FaiI	YATR	4 cut(s)	78, 158, 347, 414
FatI	CATG	2 cut(s)	156, 412
FauI	CCCGC	1 cut(s)	514
Fnu4HI	GCNGC	2 cut(s)	249, 462
Fsp4HI	GCNGC	2 cut(s)	249, 462
FspBI	CTAG	1 cut(s)	207
GlaI	GCGC	1 cut(s)	215
GluI	GCNGC	2 cut(s)	249, 462
GsuI	CTGGAG	2 cut(s)	90, 474
HaeIII	GGCC	1 cut(s)	201
HapII	CCGG	2 cut(s)	465, 473
HhaI	GCGC	1 cut(s)	216
Hin1II	CATG	2 cut(s)	160, 416
Hin6I	GCGC	1 cut(s)	214
HinP1I	GCGC	1 cut(s)	214
HindIII	AAGCTT	1 cut(s)	392
HinfI	GANTC	1 cut(s)	431
HpaII	CCGG	2 cut(s)	465, 473
Hpy188I	TCNGA	2 cut(s)	164, 178
Hpy188III	TCNNGA	5 cut(s)	8, 107, 365, 386, 441
HpyAV	CCTTC	3 cut(s)	427, 442, 586
HpyCH4III	ACNGT	2 cut(s)	56, 135
HpyCH4V	TGCA	2 cut(s)	251, 345
HpyF10VI	GCNNNNNNNGC	1 cut(s)	458
HpyF3I	CTNAG	1 cut(s)	42
Hsp92II	CATG	2 cut(s)	160, 416
HspAI	GCGC	1 cut(s)	214
Kzo9I	GATC	1 cut(s)	367
LmnI	GCTCC	2 cut(s)	268, 524
LpnPI	CCDG	8 cut(s)	54, 120, 378, 413, 438, 478, 486, 560
Lsp1109I	GCAGC	2 cut(s)	235, 473
MaeI	CTAG	1 cut(s)	207
MaeIII	GTNAC	1 cut(s)	253
MalI	GATC	1 cut(s)	369
MboI	GATC	1 cut(s)	367
MboII	GAAGA	4 cut(s)	73, 177, 590, 601
MluCI	AATT	3 cut(s)	47, 321, 560
MnlI	CCTC	7 cut(s)	37, 65, 110, 331, 421, 496, 538
Mph1103I	ATGCAT	1 cut(s)	347
MseI	TTAA	5 cut(s)	21, 195, 396, 516, 540
MspCI	CTTAAG	1 cut(s)	194
MspI	CCGG	2 cut(s)	465, 473
Mva1269I	GAATGC	2 cut(s)	347, 474
MwoI	GCNNNNNNNGC	1 cut(s)	458
NdeII	GATC	1 cut(s)	367
NlaIII	CATG	2 cut(s)	160, 416
NlaIV	GGNNCC	3 cut(s)	33, 497, 526
NmeAIII	GCCGAG	1 cut(s)	304
NsiI	ATGCAT	1 cut(s)	347
NspI	RCATGY	1 cut(s)	416
PaeI	GCATGC	1 cut(s)	416
PctI	GAATGC	2 cut(s)	347, 474
PfeI	GAWTC	1 cut(s)	431
PkrI	GCNGC	2 cut(s)	250, 463
PshBI	ATTAAT	1 cut(s)	516
PspN4I	GGNNCC	3 cut(s)	33, 497, 526
PspPI	GGNCC	1 cut(s)	199
PstI	CTGCAG	1 cut(s)	253
SaqAI	TTAA	5 cut(s)	21, 195, 396, 516, 540
SatI	GCNGC	2 cut(s)	249, 462
Sau3AI	GATC	1 cut(s)	367
Sau96I	GGNCC	1 cut(s)	199
SetI	ASST	9 cut(s)	19, 76, 208, 212, 285, 306, 396, 402, 597
SfcI	CTRYAG	2 cut(s)	52, 249
SmlI	CTYRAG	2 cut(s)	194, 238
SmoI	CTYRAG	2 cut(s)	194, 238
SphI	GCATGC	1 cut(s)	416
Sse9I	AATT	3 cut(s)	47, 321, 560
SsiI	CCGC	1 cut(s)	521
SspI	AATATT	1 cut(s)	288
SspMI	CTAG	1 cut(s)	207
StyI	CCWWGG	1 cut(s)	206
TaaI	ACNGT	2 cut(s)	56, 135
TaqI	TCGA	1 cut(s)	120
TasI	AATT	3 cut(s)	47, 321, 560
TfiI	GAWTC	1 cut(s)	431
Tru1I	TTAA	5 cut(s)	21, 195, 396, 516, 540
Tru9I	TTAA	5 cut(s)	21, 195, 396, 516, 540
TscAI	CASTG	3 cut(s)	46, 61, 579
TseI	GCWGC	2 cut(s)	248, 461
TspDTI	ATGAA	1 cut(s)	409
TspGWI	ACGGA	1 cut(s)	323
TspRI	CASTG	3 cut(s)	46, 61, 579
Vha464I	CTTAAG	1 cut(s)	194
VspI	ATTAAT	1 cut(s)	516
XapI	RAATTY	1 cut(s)	47
XceI	RCATGY	1 cut(s)	416
XmaJI	CCTAGG	1 cut(s)	206
XspI	CTAG	1 cut(s)	207
Zsp2I	ATGCAT	1 cut(s)	347

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.