Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0004871

Physical Location & Seq

Genomic Coordinates Forward (+)

26548043 .. 26550777

Gene Structure 2735 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1226200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 744 bp

ATGGCCTCCCTTTCATCAAATGCTCACCCGCTCGATAGAAAATCTTCTCTAGCTTGTGTCGCACCTCTAGAAGCAATTCTATTTGATATTGATGGAACATTGTGTGATTCAGATCCCTTCCATTATTATGCTTTCCGTGAAATGCTTCAAGAAGTAGGTTTTAATGGTGGGGTTCCTATCACTGAGGAATTCTTCAGTGAGAAGATAGCTGGAGTTCATAATGAGTATCTTTGTAGTATCCTCTTTCCTGATTGGGATATCGAAAGAGCAAGACAGTTTTTGGTAGATAAGGAAGCCATGTTCCGAAGATTTATATCTGAAAAGATAGAACCTCTTAAGGGCCTCCCTAGGTTGCGCCAATGGATTGAAAAACCAGGCTTCAGAAGGGCTGCAGTAACAAATGCTACAAAACTAAATGCCGAGCTAATATTATCAAAGTTGAAGCTCACGGATTTCTTTGAAATTGTTGTTCTTGGAGAGGAATGCATACGAGCAAAACCATTTCCTGATCCTTACTTGACGGCTCTTGAAAAGCTTAACCTGTCAAACGAGCATGCTTTCATCTTTGAGGATTCTGTTTTGGGAGTGAAGGCTGGAGTAGCAGCCGGAATGCCAGTAGTGGGTTTAGGCACAAGGAACCCCGAGGAAGCATTGATTAATGCGGGAGCCTCCTTTGTTATTAAAGATTTTGATGACCCAAAATTGTGGGAAGCACTGGAAGAGATTGTGAAGAAGGTGGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HAD_2

Protein Analysis

248

Amino Acids

27.74

Weight (kDa)

4.99

Isoelectric Point (pI)

47.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hydrolase	PF00702	24 - 203	2.4e-22	haloacid dehalogenase-like hydrolase
HAD_2	PF13419	26 - 208	1.1e-26	Haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0004871)

Species	Orthologous Gene IDs
malus_domestica	MD00G1123600.v1.1 MD00G1190500.v1.1 MD02G1226100.v1.1 MD02G1226200.v1.1 MD02G1226400.v1.1 MD07G1087800.v1.1
prunus_persica	Prupe.2G111900_v2.0.a1 Prupe.2G114400_v2.0.a1
pyrus_communis	pycom02g19470 pycom02g19500
rosa_chinensis	RchiOBHm_Chr1g0343701 RchiOBHm_Chr1g0343751 RchiOBHm_Chr1g0343881 RchiOBHm_Chr1g0360261
rosa_laevigata	RLG00000028950 RLG00000028952
rosa_multiflora	Rmu_sc0004270.1_g000007 Rmu_sc0007032.1_g000015 Rmu_ssc0000259.1_g000047
rosa_roxburghii	Rroxscaffold_4G00310310
rosa_rugosa	Rorug01G0168900 Rorug01G0169000
rosa_samantha	Rh1AG185100 Rh1AG185500 Rh1BG125700 Rh1BG152400 Rh1CG171300 Rh1DG184400 Rh6BG211900
rosa_wichuraiana	Rw1G015310 Rw1G015350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	31
AciI	CCGC	2 cut(s)	29, 662
AclWI	GGATC	2 cut(s)	107, 503
AcsI	RAATTY	1 cut(s)	188
AcuI	CTGAAG	2 cut(s)	178, 364
AfiI	CCNNNNNNNGG	2 cut(s)	338, 620
AflII	CTTAAG	1 cut(s)	335
AgsI	TTSAA	5 cut(s)	149, 368, 442, 461, 530
AjnI	CCWGG	1 cut(s)	373
AloI	GAACNNNNNNTCC	2 cut(s)	284, 316
AluBI	AGCT	5 cut(s)	53, 209, 424, 445, 535
AluI	AGCT	5 cut(s)	53, 209, 424, 445, 535
AlwI	GGATC	2 cut(s)	107, 503
Ama87I	CYCGRG	1 cut(s)	641
AoxI	GGCC	2 cut(s)	3, 340
ApeKI	GCWGC	2 cut(s)	389, 602
ApoI	RAATTY	1 cut(s)	188
AseI	ATTAAT	1 cut(s)	657
Asp700I	GAANNNNTTC	3 cut(s)	43, 75, 144
AspA2I	CCTAGG	1 cut(s)	347
AspLEI	GCGC	1 cut(s)	357
AspS9I	GGNCC	1 cut(s)	340
AsuHPI	GGTGA	1 cut(s)	17
AvaI	CYCGRG	1 cut(s)	641
AvrII	CCTAGG	1 cut(s)	347
BbvI	GCAGC	2 cut(s)	376, 614
BccI	CCATC	1 cut(s)	86
BceAI	ACGGC	1 cut(s)	537
BciT130I	CCWGG	1 cut(s)	375
BciVI	GTATCC	1 cut(s)	248
BfaI	CTAG	3 cut(s)	50, 68, 348
BfmI	CTRYAG	1 cut(s)	390
BfrI	CTTAAG	1 cut(s)	335
BfuI	GTATCC	1 cut(s)	248
BisI	GCNGC	2 cut(s)	390, 603
BlnI	CCTAGG	1 cut(s)	347
BlsI	GCNGC	2 cut(s)	391, 604
Bme1390I	CCNGG	1 cut(s)	375
BmeT110I	CYCGRG	1 cut(s)	641
BmgT120I	GGNCC	1 cut(s)	340
BmiI	GGNNCC	3 cut(s)	174, 638, 667
BmrFI	CCNGG	1 cut(s)	375
BpmI	CTGGAG	2 cut(s)	231, 615
BsaBI	GATNNNNATC	2 cut(s)	111, 313
BsaJI	CCNNGG	2 cut(s)	347, 642
Bsc4I	CCNNNNNNNGG	2 cut(s)	338, 620
Bse1I	ACTGG	2 cut(s)	614, 720
Bse8I	GATNNNNATC	2 cut(s)	111, 313
BseBI	CCWGG	1 cut(s)	375
BseDI	CCNNGG	2 cut(s)	347, 642
BseJI	GATNNNNATC	2 cut(s)	111, 313
BseLI	CCNNNNNNNGG	2 cut(s)	338, 620
BseMII	CTCAG	1 cut(s)	174
BseNI	ACTGG	2 cut(s)	614, 720
BseXI	GCAGC	2 cut(s)	376, 614
BshFI	GGCC	2 cut(s)	5, 342
BsiHKCI	CYCGRG	1 cut(s)	641
BsiSI	CCGG	1 cut(s)	606
BslI	CCNNNNNNNGG	2 cut(s)	338, 620
BsmI	GAATGC	2 cut(s)	488, 615
BsnI	GGCC	2 cut(s)	5, 342
BsoBI	CYCGRG	1 cut(s)	641
Bsp143I	GATC	2 cut(s)	112, 508
BspACI	CCGC	2 cut(s)	29, 662
BspANI	GGCC	2 cut(s)	5, 342
BspCNI	CTCAG	1 cut(s)	175
BspLI	GGNNCC	3 cut(s)	174, 638, 667
BspMAI	CTGCAG	1 cut(s)	394
BspPI	GGATC	2 cut(s)	107, 503
BspTI	CTTAAG	1 cut(s)	335
BsrBI	CCGCTC	1 cut(s)	31
BsrI	ACTGG	2 cut(s)	614, 720
BssECI	CCNNGG	2 cut(s)	347, 642
BssMI	GATC	2 cut(s)	112, 508
BssT1I	CCWWGG	1 cut(s)	347
Bst2UI	CCWGG	1 cut(s)	375
Bst4CI	ACNGT	1 cut(s)	276
Bst6I	CTCTTC	1 cut(s)	714
BstAFI	CTTAAG	1 cut(s)	335
BstC8I	GCNNGC	1 cut(s)	555
BstDEI	CTNAG	1 cut(s)	183
BstHHI	GCGC	1 cut(s)	357
BstKTI	GATC	2 cut(s)	115, 511
BstMBI	GATC	2 cut(s)	112, 508
BstMWI	GCNNNNNNNGC	2 cut(s)	59, 599
BstNI	CCWGG	1 cut(s)	375
BstNSI	RCATGY	1 cut(s)	557
BstSCI	CCNGG	1 cut(s)	373
BstSFI	CTRYAG	1 cut(s)	390
BstV1I	GCAGC	2 cut(s)	376, 614
BstX2I	RGATCY	1 cut(s)	112
BstXI	CCANNNNNNTGG	1 cut(s)	705
BstYI	RGATCY	1 cut(s)	112
BsuI	GTATCC	1 cut(s)	248
BsuRI	GGCC	2 cut(s)	5, 342
BtsIMutI	CAGTG	3 cut(s)	180, 202, 713
Cac8I	GCNNGC	1 cut(s)	555
CfoI	GCGC	1 cut(s)	357
Cfr13I	GGNCC	1 cut(s)	340
CviAII	CATG	2 cut(s)	298, 554
DdeI	CTNAG	1 cut(s)	183
DpnI	GATC	2 cut(s)	114, 510
DpnII	GATC	2 cut(s)	112, 508
Eam1104I	CTCTTC	1 cut(s)	714
EarI	CTCTTC	1 cut(s)	714
Eco130I	CCWWGG	1 cut(s)	347
Eco32I	GATATC	1 cut(s)	259
Eco57I	CTGAAG	2 cut(s)	178, 364
Eco88I	CYCGRG	1 cut(s)	641
EcoO109I	RGGNCCY	1 cut(s)	340
EcoRI	GAATTC	1 cut(s)	188
EcoRII	CCWGG	1 cut(s)	373
EcoRV	GATATC	1 cut(s)	259
EcoT14I	CCWWGG	1 cut(s)	347
EcoT22I	ATGCAT	1 cut(s)	488
ErhI	CCWWGG	1 cut(s)	347
FaeI	CATG	2 cut(s)	301, 557
FaiI	YATR	6 cut(s)	129, 219, 299, 314, 488, 555
FatI	CATG	2 cut(s)	297, 553
FauI	CCCGC	2 cut(s)	36, 655
Fnu4HI	GCNGC	2 cut(s)	390, 603
Fsp4HI	GCNGC	2 cut(s)	390, 603
FspBI	CTAG	3 cut(s)	50, 68, 348
GlaI	GCGC	1 cut(s)	356
GluI	GCNGC	2 cut(s)	390, 603
GsuI	CTGGAG	2 cut(s)	231, 615
HaeIII	GGCC	2 cut(s)	5, 342
HapII	CCGG	1 cut(s)	606
HhaI	GCGC	1 cut(s)	357
Hin1II	CATG	2 cut(s)	301, 557
Hin6I	GCGC	1 cut(s)	355
HinP1I	GCGC	1 cut(s)	355
HindIII	AAGCTT	1 cut(s)	533
HinfI	GANTC	2 cut(s)	107, 572
HpaII	CCGG	1 cut(s)	606
HphI	GGTGA	1 cut(s)	17
Hpy188I	TCNGA	4 cut(s)	112, 305, 319, 383
Hpy188III	TCNNGA	5 cut(s)	68, 149, 248, 506, 527
HpyAV	CCTTC	4 cut(s)	127, 378, 583, 727
HpyCH4III	ACNGT	1 cut(s)	276
HpyCH4V	TGCA	2 cut(s)	392, 486
HpyF10VI	GCNNNNNNNGC	2 cut(s)	59, 599
HpyF3I	CTNAG	1 cut(s)	183
Hsp92II	CATG	2 cut(s)	301, 557
HspAI	GCGC	1 cut(s)	355
Kzo9I	GATC	2 cut(s)	112, 508
LmnI	GCTCC	1 cut(s)	665
Lsp1109I	GCAGC	2 cut(s)	376, 614
MaeI	CTAG	3 cut(s)	50, 68, 348
MaeIII	GTNAC	1 cut(s)	394
MalI	GATC	2 cut(s)	114, 510
MbiI	CCGCTC	1 cut(s)	31
MboI	GATC	2 cut(s)	112, 508
MboII	GAAGA	6 cut(s)	36, 184, 214, 318, 731, 742
MflI	RGATCY	1 cut(s)	112
MluCI	AATT	4 cut(s)	75, 188, 462, 701
Mph1103I	ATGCAT	1 cut(s)	488
MroXI	GAANNNNTTC	3 cut(s)	43, 75, 144
MseI	TTAA	5 cut(s)	162, 336, 537, 657, 681
MslI	CAYNNNNRTG	1 cut(s)	126
MspCI	CTTAAG	1 cut(s)	335
MspI	CCGG	1 cut(s)	606
MspR9I	CCNGG	1 cut(s)	375
Mva1269I	GAATGC	2 cut(s)	488, 615
MvaI	CCWGG	1 cut(s)	375
MwoI	GCNNNNNNNGC	2 cut(s)	59, 599
NdeII	GATC	2 cut(s)	112, 508
NlaIII	CATG	2 cut(s)	301, 557
NlaIV	GGNNCC	3 cut(s)	174, 638, 667
NmeAIII	GCCGAG	1 cut(s)	445
NsiI	ATGCAT	1 cut(s)	488
NspI	RCATGY	1 cut(s)	557
PaeI	GCATGC	1 cut(s)	557
PctI	GAATGC	2 cut(s)	488, 615
PdmI	GAANNNNTTC	3 cut(s)	43, 75, 144
PfeI	GAWTC	2 cut(s)	107, 572
PkrI	GCNGC	2 cut(s)	391, 604
PshBI	ATTAAT	1 cut(s)	657
Psp6I	CCWGG	1 cut(s)	373
PspGI	CCWGG	1 cut(s)	373
PspN4I	GGNNCC	3 cut(s)	174, 638, 667
PspPI	GGNCC	1 cut(s)	340
PstI	CTGCAG	1 cut(s)	394
PsuI	RGATCY	1 cut(s)	112
RseI	CAYNNNNRTG	1 cut(s)	126
SaqAI	TTAA	5 cut(s)	162, 336, 537, 657, 681
SatI	GCNGC	2 cut(s)	390, 603
Sau3AI	GATC	2 cut(s)	112, 508
Sau96I	GGNCC	1 cut(s)	340
ScrFI	CCNGG	1 cut(s)	375
SfcI	CTRYAG	1 cut(s)	390
SmiMI	CAYNNNNRTG	1 cut(s)	126
SmlI	CTYRAG	1 cut(s)	335
SmoI	CTYRAG	1 cut(s)	335
SphI	GCATGC	1 cut(s)	557
Sse9I	AATT	4 cut(s)	75, 188, 462, 701
SsiI	CCGC	2 cut(s)	29, 662
SspI	AATATT	1 cut(s)	429
SspMI	CTAG	3 cut(s)	50, 68, 348
StyD4I	CCNGG	1 cut(s)	373
StyI	CCWWGG	1 cut(s)	347
TaaI	ACNGT	1 cut(s)	276
TaqI	TCGA	2 cut(s)	33, 261
TasI	AATT	4 cut(s)	75, 188, 462, 701
TfiI	GAWTC	2 cut(s)	107, 572
Tru1I	TTAA	5 cut(s)	162, 336, 537, 657, 681
Tru9I	TTAA	5 cut(s)	162, 336, 537, 657, 681
TscAI	CASTG	3 cut(s)	187, 202, 720
TseI	GCWGC	2 cut(s)	389, 602
TspDTI	ATGAA	2 cut(s)	206, 550
TspGWI	ACGGA	2 cut(s)	125, 464
TspRI	CASTG	3 cut(s)	187, 202, 720
Vha464I	CTTAAG	1 cut(s)	335
VspI	ATTAAT	1 cut(s)	657
XapI	RAATTY	1 cut(s)	188
XbaI	TCTAGA	1 cut(s)	67
XceI	RCATGY	1 cut(s)	557
XmaJI	CCTAGG	1 cut(s)	347
XmnI	GAANNNNTTC	3 cut(s)	43, 75, 144
XspI	CTAG	3 cut(s)	50, 68, 348
Zsp2I	ATGCAT	1 cut(s)	488

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.