MD01G1075400.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0006158
Physical Location & Seq
Forward (+)
18127549 .. 18128397
849 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1075400.v1.1.491

Sequence Viewer

Length: 849 bp
ATGGATCGACCCAAGCAGCACACGCAGCGTCGCACGGTCAAAAACAAGCTTGTCAAAAAGCTCACCACCCGCTTCCGGGAGTCTACTGCCGGAGCCCCCCAAATCGTAAGGATTTCCGTCACCGACGACAACGCCACCGACTCCTCCAGCGACGAAGCCGAAGCCGAGCCGGGCCGGAAATACCGGGTCCTCATGAAGCGCGTCATCAACGAGGTCAGAATCGAAGACCCGTCTCCTTCTCAGGCGCTCGTCTCAAAACCAACTAAGCCTTCTAGGTGCAGAGCGGCGGAGGAGAGTAACGAGATTTCGCCCAACGGGAAGAAATACCGAGGCGTACGGCAGAGGCCGTGGGGGAAATGGGCGGCGGAGATCCGAGACCCAAAACGTGGGGTCCGGGTCTGGCTCGGAACTTTCGAGACGGCGGAGGAGGCCGGTATGGCTTACGATAAGGCTGCCATTGCGTTTAAGGGACCTCTGGCTTTGACCAATTTCCTCAAGCCTCCCGAAATTGCCATTCCGGACATCTCGGAAGAGGACTGCAACCCAATTTCTCAGTCTCCGCTCAACTCGCGGTGCGGCCACAGCGTCCGGTCGCCAACCTCTGTTCTCAGTGGCCGAGAACCCGAAATCGACTGGAAAGTTTCGGAGCGACAAAATTTCGGGGTTTTTGATACGGAGTTTTGGTTGAATGATTGTTTTGAACCGCCGATATTTTTCGAGGAAACGAGAGTCGTACCCGAAACGCTTCTGAAAGATGAGGATTTCTCCGATTTATCGCTTGATTTCCGGACGTGTAATTGGGATGACGTGGAGGATTACTTTCAAGATGCTTATGAGTTGAAACGATGA

Protein Analysis

283

Amino Acids

32.24

Weight (kDa)

5.56

Isoelectric Point (pI)

48.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 108 - 157 1.1e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 386
AccBSI CCGCTC 2 cut(s) 284, 562
AccI GTMKAC 1 cut(s) 83
AccII CGCG 2 cut(s) 201, 571
AccIII TCCGGA 2 cut(s) 517, 786
AclWI GGATC 2 cut(s) 12, 364
AcoI YGGCCR 2 cut(s) 577, 613
AcsI RAATTY 1 cut(s) 655
AfaI GTAC 2 cut(s) 336, 735
AfiI CCNNNNNNNGG 3 cut(s) 75, 76, 386
AflIII ACRYGT 1 cut(s) 791
AgsI TTSAA 4 cut(s) 688, 701, 824, 841
AjiI CACGTC 2 cut(s) 792, 808
AjuI GAANNNNNNNTTGG 2 cut(s) 253, 285
AluBI AGCT 2 cut(s) 49, 61
AluI AGCT 2 cut(s) 49, 61
Alw26I GTCTC 5 cut(s) 237, 256, 369, 410, 561
AlwI GGATC 2 cut(s) 12, 364
Aor13HI TCCGGA 2 cut(s) 517, 786
AoxI GGCC 5 cut(s) 172, 344, 429, 577, 613
ApeKI GCWGC 3 cut(s) 16, 25, 452
ApoI RAATTY 1 cut(s) 655
Asp700I GAANNNNTTC 1 cut(s) 744
AspLEI GCGC 2 cut(s) 201, 247
AspS9I GGNCC 4 cut(s) 172, 187, 391, 470
AsuC2I CCSGG 4 cut(s) 77, 171, 185, 395
AsuHPI GGTGA 2 cut(s) 55, 112
AvaII GGWCC 3 cut(s) 187, 391, 470
BanII GRGCYC 1 cut(s) 97
BbsI GAAGAC 1 cut(s) 231
BbvI GCAGC 3 cut(s) 28, 37, 439
BceAI ACGGC 3 cut(s) 331, 353, 435
BcgI CGANNNNNNTGC 2 cut(s) 434, 468
BcnI CCSGG 4 cut(s) 77, 171, 185, 395
BcoDI GTCTC 5 cut(s) 237, 256, 369, 410, 561
BfaI CTAG 1 cut(s) 273
BfoI RGCGCY 1 cut(s) 248
BglI GCCNNNNNGGC 1 cut(s) 437
BisI GCNGC 6 cut(s) 17, 26, 285, 363, 453, 577
BlsI GCNGC 6 cut(s) 18, 27, 286, 364, 454, 578
Bme1390I CCNGG 4 cut(s) 77, 171, 185, 395
Bme18I GGWCC 3 cut(s) 187, 391, 470
BmgBI CACGTC 2 cut(s) 792, 808
BmgT120I GGNCC 4 cut(s) 172, 187, 391, 470
BmiI GGNNCC 4 cut(s) 94, 188, 392, 471
BmrFI CCNGG 4 cut(s) 77, 171, 185, 395
BmsI GCATC 1 cut(s) 817
BpiI GAAGAC 1 cut(s) 231
BplI GAGNNNNNCTC 2 cut(s) 749, 781
BpmI CTGGAG 1 cut(s) 130
BpuEI CTTGAG 1 cut(s) 479
BpuMI CCSGG 4 cut(s) 77, 171, 185, 395
BsaI GGTCTC 1 cut(s) 369
BsaJI CCNNGG 2 cut(s) 328, 347
BsaWI WCCGGW 3 cut(s) 517, 588, 786
BsaXI ACNNNNNCTCC 2 cut(s) 668, 698
Bsc4I CCNNNNNNNGG 3 cut(s) 75, 76, 386
Bse118I RCCGGY 1 cut(s) 431
Bse1I ACTGG 1 cut(s) 638
Bse3DI GCAATG 1 cut(s) 456
BseAI TCCGGA 2 cut(s) 517, 786
BseDI CCNNGG 2 cut(s) 328, 347
BseGI GGATG 1 cut(s) 808
BseLI CCNNNNNNNGG 3 cut(s) 75, 76, 386
BseMI GCAATG 1 cut(s) 456
BseMII CTCAG 3 cut(s) 254, 566, 622
BseNI ACTGG 1 cut(s) 638
BseRI GAGGAG 3 cut(s) 133, 305, 440
BseXI GCAGC 3 cut(s) 28, 37, 439
BsgI GTGCAG 1 cut(s) 298
Bsh1236I CGCG 2 cut(s) 201, 571
Bsh1285I CGRYCG 1 cut(s) 593
BshFI GGCC 5 cut(s) 174, 346, 431, 579, 615
BsiEI CGRYCG 1 cut(s) 593
BsiWI CGTACG 1 cut(s) 334
BslFI GGGAC 1 cut(s) 483
BslI CCNNNNNNNGG 3 cut(s) 75, 76, 386
BsmAI GTCTC 5 cut(s) 237, 256, 369, 410, 561
BsmBI CGTCTC 3 cut(s) 237, 256, 410
BsmFI GGGAC 1 cut(s) 483
BsnI GGCC 5 cut(s) 174, 346, 431, 579, 615
Bso31I GGTCTC 1 cut(s) 369
Bsp1286I GDGCHC 1 cut(s) 97
Bsp13I TCCGGA 2 cut(s) 517, 786
Bsp143I GATC 2 cut(s) 4, 369
BspANI GGCC 5 cut(s) 174, 346, 431, 579, 615
BspCNI CTCAG 3 cut(s) 253, 565, 621
BspEI TCCGGA 2 cut(s) 517, 786
BspFNI CGCG 2 cut(s) 201, 571
BspHI TCATGA 1 cut(s) 192
BspLI GGNNCC 4 cut(s) 94, 188, 392, 471
BspPI GGATC 2 cut(s) 12, 364
BspTNI GGTCTC 1 cut(s) 369
BsrBI CCGCTC 2 cut(s) 284, 562
BsrDI GCAATG 1 cut(s) 456
BsrFI RCCGGY 1 cut(s) 431
BsrI ACTGG 1 cut(s) 638
BssAI RCCGGY 1 cut(s) 431
BssECI CCNNGG 2 cut(s) 328, 347
BssMI GATC 2 cut(s) 4, 369
Bst4CI ACNGT 1 cut(s) 37
Bst6I CTCTTC 1 cut(s) 525
BstDEI CTNAG 4 cut(s) 240, 264, 552, 608
BstDSI CCRYGG 1 cut(s) 347
BstF5I GGATG 1 cut(s) 808
BstFNI CGCG 2 cut(s) 201, 571
BstH2I RGCGCY 1 cut(s) 248
BstHHI GCGC 2 cut(s) 201, 247
BstKTI GATC 2 cut(s) 7, 372
BstMAI GTCTC 5 cut(s) 237, 256, 369, 410, 561
BstMBI GATC 2 cut(s) 4, 369
BstMCI CGRYCG 1 cut(s) 593
BstMWI GCNNNNNNNGC 7 cut(s) 22, 25, 428, 437, 458, 568, 582
BstSCI CCNGG 4 cut(s) 75, 169, 183, 393
BstUI CGCG 2 cut(s) 201, 571
BstV1I GCAGC 3 cut(s) 28, 37, 439
BstV2I GAAGAC 1 cut(s) 231
BstX2I RGATCY 1 cut(s) 369
BstYI RGATCY 1 cut(s) 369
BsuRI GGCC 5 cut(s) 174, 346, 431, 579, 615
BtgI CCRYGG 1 cut(s) 347
BtrI CACGTC 2 cut(s) 792, 808
BtsCI GGATG 1 cut(s) 808
BtsIMutI CAGTG 1 cut(s) 616
CciI TCATGA 1 cut(s) 192
CfoI GCGC 2 cut(s) 201, 247
Cfr10I RCCGGY 1 cut(s) 431
Cfr13I GGNCC 4 cut(s) 172, 187, 391, 470
CseI GACGC 3 cut(s) 17, 190, 574
Csp6I GTAC 2 cut(s) 335, 734
CviAII CATG 1 cut(s) 193
CviQI GTAC 2 cut(s) 335, 734
DdeI CTNAG 4 cut(s) 240, 264, 552, 608
DpnI GATC 2 cut(s) 6, 371
DpnII GATC 2 cut(s) 4, 369
EaeI YGGCCR 2 cut(s) 577, 613
Eam1104I CTCTTC 1 cut(s) 525
EarI CTCTTC 1 cut(s) 525
EciI GGCGGA 3 cut(s) 302, 380, 437
Eco24I GRGCYC 1 cut(s) 97
Eco31I GGTCTC 1 cut(s) 369
Eco47I GGWCC 3 cut(s) 187, 391, 470
EcoO109I RGGNCCY 2 cut(s) 187, 470
EcoT38I GRGCYC 1 cut(s) 97
Esp3I CGTCTC 3 cut(s) 237, 256, 410
FaeI CATG 1 cut(s) 196
FaiI YATR 3 cut(s) 194, 437, 834
FaqI GGGAC 1 cut(s) 483
FatI CATG 1 cut(s) 192
FauI CCCGC 1 cut(s) 77
FblI GTMKAC 1 cut(s) 83
Fnu4HI GCNGC 6 cut(s) 17, 26, 285, 363, 453, 577
FokI GGATG 1 cut(s) 815
FriOI GRGCYC 1 cut(s) 97
Fsp4HI GCNGC 6 cut(s) 17, 26, 285, 363, 453, 577
FspBI CTAG 1 cut(s) 273
GlaI GCGC 2 cut(s) 200, 246
GluI GCNGC 6 cut(s) 17, 26, 285, 363, 453, 577
GsuI CTGGAG 1 cut(s) 130
HaeII RGCGCY 1 cut(s) 248
HaeIII GGCC 5 cut(s) 174, 346, 431, 579, 615
HgaI GACGC 3 cut(s) 17, 190, 574
HhaI GCGC 2 cut(s) 201, 247
Hin1II CATG 1 cut(s) 196
Hin6I GCGC 2 cut(s) 199, 245
HinP1I GCGC 2 cut(s) 199, 245
HindIII AAGCTT 1 cut(s) 47
HinfI GANTC 4 cut(s) 80, 140, 219, 729
HphI GGTGA 2 cut(s) 55, 112
Hpy166II GTNNAC 1 cut(s) 84
Hpy188I TCNGA 7 cut(s) 218, 374, 407, 529, 646, 750, 769
Hpy188III TCNNGA 6 cut(s) 193, 415, 503, 518, 787, 824
Hpy8I GTNNAC 1 cut(s) 84
Hpy99I CGWCG 3 cut(s) 33, 128, 155
HpyAV CCTTC 2 cut(s) 246, 279
HpyCH4III ACNGT 1 cut(s) 37
HpyCH4IV ACGT 3 cut(s) 385, 791, 807
HpyCH4V TGCA 2 cut(s) 279, 540
HpyF10VI GCNNNNNNNGC 7 cut(s) 22, 25, 428, 437, 458, 568, 582
HpyF3I CTNAG 4 cut(s) 240, 264, 552, 608
HpySE526I ACGT 3 cut(s) 385, 791, 807
Hsp92II CATG 1 cut(s) 196
HspAI GCGC 2 cut(s) 199, 245
Kpn2I TCCGGA 2 cut(s) 517, 786
Kzo9I GATC 2 cut(s) 4, 369
LmnI GCTCC 2 cut(s) 92, 646
Lsp1109I GCAGC 3 cut(s) 28, 37, 439
LweI GCATC 1 cut(s) 817
MaeI CTAG 1 cut(s) 273
MaeII ACGT 3 cut(s) 385, 791, 807
MaeIII GTNAC 2 cut(s) 118, 296
MalI GATC 2 cut(s) 6, 371
MbiI CCGCTC 2 cut(s) 284, 562
MboI GATC 2 cut(s) 4, 369
MboII GAAGA 3 cut(s) 236, 331, 542
MflI RGATCY 1 cut(s) 369
MhlI GDGCHC 1 cut(s) 97
MluCI AATT 5 cut(s) 487, 507, 546, 655, 796
MlyI GAGTC 3 cut(s) 89, 134, 738
MroI TCCGGA 2 cut(s) 517, 786
MroXI GAANNNNTTC 1 cut(s) 744
MseI TTAA 1 cut(s) 465
MspR9I CCNGG 4 cut(s) 77, 171, 185, 395
MvnI CGCG 2 cut(s) 201, 571
MwoI GCNNNNNNNGC 7 cut(s) 22, 25, 428, 437, 458, 568, 582
NciI CCSGG 4 cut(s) 77, 171, 185, 395
NdeII GATC 2 cut(s) 4, 369
NlaIII CATG 1 cut(s) 196
NlaIV GGNNCC 4 cut(s) 94, 188, 392, 471
NmeAIII GCCGAG 2 cut(s) 190, 641
NmuCI GTSAC 1 cut(s) 118
PagI TCATGA 1 cut(s) 192
PcsI WCGNNNNNNNCGW 1 cut(s) 411
PdmI GAANNNNTTC 1 cut(s) 744
PfeI GAWTC 1 cut(s) 219
Pfl23II CGTACG 1 cut(s) 334
PflMI CCANNNNNTGG 1 cut(s) 386
PfoI TCCNGGA 1 cut(s) 75
PkrI GCNGC 6 cut(s) 18, 27, 286, 364, 454, 578
PleI GAGTC 3 cut(s) 88, 134, 737
PpsI GAGTC 3 cut(s) 88, 134, 737
PpuMI RGGWCCY 2 cut(s) 187, 470
Psp5II RGGWCCY 2 cut(s) 187, 470
PspLI CGTACG 1 cut(s) 334
PspN4I GGNNCC 4 cut(s) 94, 188, 392, 471
PspPI GGNCC 4 cut(s) 172, 187, 391, 470
PspPPI RGGWCCY 2 cut(s) 187, 470
PsuI RGATCY 1 cut(s) 369
RsaI GTAC 2 cut(s) 336, 735
RsaNI GTAC 2 cut(s) 335, 734
SaqAI TTAA 1 cut(s) 465
SatI GCNGC 6 cut(s) 17, 26, 285, 363, 453, 577
Sau3AI GATC 2 cut(s) 4, 369
Sau96I GGNCC 4 cut(s) 172, 187, 391, 470
SchI GAGTC 3 cut(s) 89, 134, 738
ScrFI CCNGG 4 cut(s) 77, 171, 185, 395
SduI GDGCHC 1 cut(s) 97
SetI ASST 9 cut(s) 51, 63, 216, 278, 388, 475, 602, 794, 810
SfaNI GCATC 1 cut(s) 817
SinI GGWCC 3 cut(s) 187, 391, 470
SmlI CTYRAG 1 cut(s) 494
SmoI CTYRAG 1 cut(s) 494
Sse9I AATT 5 cut(s) 487, 507, 546, 655, 796
SspMI CTAG 1 cut(s) 273
StyD4I CCNGG 4 cut(s) 75, 169, 183, 393
TaaI ACNGT 1 cut(s) 37
TaiI ACGT 3 cut(s) 388, 794, 810
TaqI TCGA 5 cut(s) 7, 222, 414, 630, 717
TasI AATT 5 cut(s) 487, 507, 546, 655, 796
TauI GCSGC 3 cut(s) 287, 365, 579
TfiI GAWTC 1 cut(s) 219
Tru1I TTAA 1 cut(s) 465
Tru9I TTAA 1 cut(s) 465
TscAI CASTG 1 cut(s) 616
TseFI GTSAC 1 cut(s) 118
TseI GCWGC 3 cut(s) 16, 25, 452
Tsp45I GTSAC 1 cut(s) 118
TspDTI ATGAA 1 cut(s) 209
TspGWI ACGGA 2 cut(s) 106, 689
TspRI CASTG 1 cut(s) 616
Van91I CCANNNNNTGG 1 cut(s) 386
VpaK11BI GGWCC 3 cut(s) 187, 391, 470
XapI RAATTY 1 cut(s) 655
XmiI GTMKAC 1 cut(s) 83
XmnI GAANNNNTTC 1 cut(s) 744
XspI CTAG 1 cut(s) 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.