RchiOBHm_Chr2g0166851

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0006158
Physical Location & Seq
Forward (+)
81578693 .. 81579766
1074 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53467

Sequence Viewer

Length: 1074 bp
ATGCCACGAATAATTTTTATACATAAAGTTTCAAACCAAACATTTCGGTACACGAAATCTGAAACTCAACTCACTATCAGTACACGACGTCAATTTTTGACACCAAAATGTCCATTATGCCCTCAGTTCTTTTTTTTTTTTAATAATTTTTTTTCCGGCACCAAGTTCATTTTCGTCACAGACGAGGACGCGACCGTTGCAGTCCCTAAGATTGTCCGGATTTCAATCACGGACGAGGACGCGACCAACACCTCCAGCAACGAAGAAGAACAAGACCAACAACAACGACTGACGAAGAGGGTCATCAACGAAGTGAGAATTGAAGAGGAGTCCGAGGTGGTCAGGGTGTTTCTGGCCAATAATCAGAAGCAAAAGTCGAAGCCTTTACCGGAGGAGAAGAGGAAGTTCTGGGGCGTACGGCAGCGGCGGTGGGGCAAATGGGCTGCCGAGATTCATGACCCGGTCCGCAAGTCCAGTCTCGGGCTCGGAACGTTTCAAACAGCGGAGGAGGCGGCGATGGTGTACGACAGAGCTGCCATTCAGTTGAGGGGACATAACGCTCTCACGAATATCATCGCTCCTCCTTCCAAAATTCTCATTCCGGATCACGATTCTTCTTCGAATGAGTCCAGTTCGATCAACAATCTTTCTTGTTTGAATGCGTCCAGTTCAATCGACCATATTGTTGTGATAGATGAGTATGATTCCGGGAAACAGTGTCAGAGCATTCGCTCCCCGACCTCTGTTCTCAGTGGTAGTGACAACGAGTGGAGACCTGGGTCGAAGGAGCAGGATGAAACCCATTTCGTTAAAGACTATGGTTACTTTGATGGTGTAATCAGTCCCACTAAAGCTATATTCTTCGGTGTTATAGAGCTGATTTTGTTGGAGGAAATGAACTTGGTGCCGGAAACAATGTTGGTGACGTCAAATTCAAAAACCCAGCTACTAAATTTAGAGCAGAACCTGTTCACGGGTGGAGGAATGGGTGTTCAATTGGAGGGCAAGGGCGGCGTTGGAAGCGAGGGAGTGAGCGTGGAGGTGAGGAAGGCAGCGTTGGAAGCGAGGGAGTGA

Protein Analysis

357

Amino Acids

40.13

Weight (kDa)

5.7

Isoelectric Point (pI)

54.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 136 - 185 1.3e-09 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 91, 929
AccB1I GGYRCC 2 cut(s) 158, 904
AccII CGCG 2 cut(s) 191, 242
AccIII TCCGGA 2 cut(s) 216, 601
AciI CCGC 6 cut(s) 424, 427, 466, 503, 512, 1011
AclI AACGTT 1 cut(s) 491
AclWI GGATC 1 cut(s) 612
AcoI YGGCCR 1 cut(s) 354
AcsI RAATTY 3 cut(s) 591, 931, 952
AcyI GRCGYC 2 cut(s) 88, 926
AfaI GTAC 4 cut(s) 50, 82, 417, 524
AfiI CCNNNNNNNGG 2 cut(s) 480, 973
AgsI TTSAA 8 cut(s) 33, 225, 323, 497, 658, 672, 936, 995
AjnI CCWGG 1 cut(s) 775
AjuI GAANNNNNNNTTGG 4 cut(s) 155, 187, 1040, 1072
AloI GAACNNNNNNTCC 2 cut(s) 975, 1007
AluBI AGCT 4 cut(s) 533, 854, 877, 946
AluI AGCT 4 cut(s) 533, 854, 877, 946
Alw26I GTCTC 2 cut(s) 482, 766
AlwI GGATC 1 cut(s) 612
AlwNI CAGNNNCTG 1 cut(s) 967
Ama87I CYCGRG 1 cut(s) 479
Aor13HI TCCGGA 2 cut(s) 216, 601
AoxI GGCC 1 cut(s) 354
ApeKI GCWGC 4 cut(s) 421, 443, 533, 1052
ApoI RAATTY 3 cut(s) 591, 931, 952
Asp700I GAANNNNTTC 1 cut(s) 968
AspS9I GGNCC 1 cut(s) 463
AsuC2I CCSGG 2 cut(s) 461, 709
AsuHPI GGTGA 2 cut(s) 934, 1054
AsuII TTCGAA 1 cut(s) 620
AvaI CYCGRG 1 cut(s) 479
AvaII GGWCC 1 cut(s) 463
BalI TGGCCA 1 cut(s) 356
BanI GGYRCC 2 cut(s) 158, 904
BanII GRGCYC 1 cut(s) 486
BbvI GCAGC 4 cut(s) 430, 433, 520, 1064
BccI CCATC 2 cut(s) 511, 824
BceAI ACGGC 1 cut(s) 434
BcgI CGANNNNNNTGC 2 cut(s) 515, 549
BciT130I CCWGG 1 cut(s) 777
BcnI CCSGG 2 cut(s) 461, 709
BcoDI GTCTC 2 cut(s) 482, 766
BisI GCNGC 7 cut(s) 422, 425, 444, 513, 534, 1012, 1053
BlsI GCNGC 7 cut(s) 423, 426, 445, 514, 535, 1013, 1054
Bme1390I CCNGG 3 cut(s) 461, 709, 777
Bme18I GGWCC 1 cut(s) 463
BmeT110I CYCGRG 1 cut(s) 479
BmgT120I GGNCC 1 cut(s) 463
BmiI GGNNCC 2 cut(s) 160, 906
BmrFI CCNGG 3 cut(s) 461, 709, 777
BoxI GACNNNNGTC 1 cut(s) 778
BpmI CTGGAG 1 cut(s) 238
Bpu14I TTCGAA 1 cut(s) 620
BpuMI CCSGG 2 cut(s) 461, 709
BsaBI GATNNNNATC 1 cut(s) 224
BsaHI GRCGYC 2 cut(s) 88, 926
BsaI GGTCTC 1 cut(s) 766
BsaJI CCNNGG 2 cut(s) 333, 776
BsaWI WCCGGW 3 cut(s) 216, 388, 601
BsaXI ACNNNNNCTCC 2 cut(s) 1020, 1050
Bsc4I CCNNNNNNNGG 2 cut(s) 480, 973
Bse1I ACTGG 3 cut(s) 474, 630, 666
Bse8I GATNNNNATC 1 cut(s) 224
BseAI TCCGGA 2 cut(s) 216, 601
BseBI CCWGG 1 cut(s) 777
BseDI CCNNGG 2 cut(s) 333, 776
BseGI GGATG 1 cut(s) 799
BseJI GATNNNNATC 1 cut(s) 224
BseLI CCNNNNNNNGG 2 cut(s) 480, 973
BseMII CTCAG 2 cut(s) 137, 763
BseNI ACTGG 3 cut(s) 474, 630, 666
BseRI GAGGAG 4 cut(s) 341, 407, 521, 570
BseXI GCAGC 4 cut(s) 430, 433, 520, 1064
BseYI CCCAGC 1 cut(s) 942
Bsh1236I CGCG 2 cut(s) 191, 242
Bsh1285I CGRYCG 1 cut(s) 195
BshFI GGCC 1 cut(s) 356
BshNI GGYRCC 2 cut(s) 158, 904
BsiEI CGRYCG 1 cut(s) 195
BsiHKCI CYCGRG 1 cut(s) 479
BsiSI CCGG 7 cut(s) 156, 217, 389, 461, 602, 708, 908
BsiWI CGTACG 1 cut(s) 415
BslFI GGGAC 3 cut(s) 188, 564, 828
BslI CCNNNNNNNGG 2 cut(s) 480, 973
BsmAI GTCTC 2 cut(s) 482, 766
BsmFI GGGAC 3 cut(s) 188, 564, 828
BsmI GAATGC 2 cut(s) 664, 726
BsnI GGCC 1 cut(s) 356
Bso31I GGTCTC 1 cut(s) 766
BsoBI CYCGRG 1 cut(s) 479
Bsp119I TTCGAA 1 cut(s) 620
Bsp1286I GDGCHC 1 cut(s) 486
Bsp13I TCCGGA 2 cut(s) 216, 601
Bsp143I GATC 2 cut(s) 604, 636
BspACI CCGC 6 cut(s) 424, 427, 466, 503, 512, 1011
BspANI GGCC 1 cut(s) 356
BspCNI CTCAG 2 cut(s) 136, 762
BspEI TCCGGA 2 cut(s) 216, 601
BspFNI CGCG 2 cut(s) 191, 242
BspHI TCATGA 1 cut(s) 454
BspLI GGNNCC 2 cut(s) 160, 906
BspPI GGATC 1 cut(s) 612
BspT104I TTCGAA 1 cut(s) 620
BspT107I GGYRCC 2 cut(s) 158, 904
BspTNI GGTCTC 1 cut(s) 766
BsrI ACTGG 3 cut(s) 474, 630, 666
BssECI CCNNGG 2 cut(s) 333, 776
BssMI GATC 2 cut(s) 604, 636
BssNI GRCGYC 2 cut(s) 88, 926
Bst2UI CCWGG 1 cut(s) 777
Bst4CI ACNGT 2 cut(s) 196, 717
Bst6I CTCTTC 3 cut(s) 290, 318, 392
BstACI GRCGYC 2 cut(s) 88, 926
BstBI TTCGAA 1 cut(s) 620
BstDEI CTNAG 3 cut(s) 123, 207, 749
BstF5I GGATG 1 cut(s) 799
BstFNI CGCG 2 cut(s) 191, 242
BstKTI GATC 2 cut(s) 607, 639
BstMAI GTCTC 2 cut(s) 482, 766
BstMBI GATC 2 cut(s) 604, 636
BstMCI CGRYCG 1 cut(s) 195
BstMWI GCNNNNNNNGC 5 cut(s) 197, 509, 1011, 1020, 1061
BstNI CCWGG 1 cut(s) 777
BstPAI GACNNNNGTC 1 cut(s) 778
BstSCI CCNGG 3 cut(s) 459, 707, 775
BstUI CGCG 2 cut(s) 191, 242
BstV1I GCAGC 4 cut(s) 430, 433, 520, 1064
BsuRI GGCC 1 cut(s) 356
BtgZI GCGATG 2 cut(s) 530, 559
BtsCI GGATG 1 cut(s) 799
BtsIMutI CAGTG 2 cut(s) 722, 757
CaiI CAGNNNCTG 1 cut(s) 967
CciI TCATGA 1 cut(s) 454
Cfr13I GGNCC 1 cut(s) 463
CpoI CGGWCCG 1 cut(s) 463
CseI GACGC 3 cut(s) 197, 248, 651
Csp6I GTAC 4 cut(s) 49, 81, 416, 523
CspI CGGWCCG 1 cut(s) 463
CviAII CATG 1 cut(s) 455
CviJI RGCY 8 cut(s) 356, 382, 443, 484, 533, 854, 877, 946
CviKI_1 RGCY 8 cut(s) 356, 382, 443, 484, 533, 854, 877, 946
CviQI GTAC 4 cut(s) 49, 81, 416, 523
DdeI CTNAG 3 cut(s) 123, 207, 749
DpnI GATC 2 cut(s) 606, 638
DpnII GATC 2 cut(s) 604, 636
EaeI YGGCCR 1 cut(s) 354
Eam1104I CTCTTC 3 cut(s) 290, 318, 392
EarI CTCTTC 3 cut(s) 290, 318, 392
Eco24I GRGCYC 1 cut(s) 486
Eco31I GGTCTC 1 cut(s) 766
Eco47I GGWCC 1 cut(s) 463
Eco88I CYCGRG 1 cut(s) 479
EcoRII CCWGG 1 cut(s) 775
EcoT38I GRGCYC 1 cut(s) 486
FaeI CATG 1 cut(s) 458
FaqI GGGAC 3 cut(s) 188, 564, 828
FatI CATG 1 cut(s) 454
Fnu4HI GCNGC 7 cut(s) 422, 425, 444, 513, 534, 1012, 1053
FokI GGATG 1 cut(s) 806
FriOI GRGCYC 1 cut(s) 486
Fsp4HI GCNGC 7 cut(s) 422, 425, 444, 513, 534, 1012, 1053
GluI GCNGC 7 cut(s) 422, 425, 444, 513, 534, 1012, 1053
GsaI CCCAGC 1 cut(s) 946
GsuI CTGGAG 1 cut(s) 238
HaeIII GGCC 1 cut(s) 356
HapII CCGG 7 cut(s) 156, 217, 389, 461, 602, 708, 908
HgaI GACGC 3 cut(s) 197, 248, 651
Hin1I GRCGYC 2 cut(s) 88, 926
Hin1II CATG 1 cut(s) 458
HinfI GANTC 5 cut(s) 329, 451, 611, 626, 704
HpaII CCGG 7 cut(s) 156, 217, 389, 461, 602, 708, 908
HphI GGTGA 2 cut(s) 934, 1054
Hpy166II GTNNAC 4 cut(s) 51, 83, 523, 972
Hpy188I TCNGA 5 cut(s) 61, 334, 366, 488, 723
Hpy188III TCNNGA 5 cut(s) 217, 455, 565, 602, 608
Hpy8I GTNNAC 4 cut(s) 51, 83, 523, 972
Hpy99I CGWCG 1 cut(s) 90
HpyAV CCTTC 3 cut(s) 594, 778, 1042
HpyCH4III ACNGT 2 cut(s) 196, 717
HpyCH4IV ACGT 3 cut(s) 88, 491, 926
HpyCH4V TGCA 1 cut(s) 200
HpyF10VI GCNNNNNNNGC 5 cut(s) 197, 509, 1011, 1020, 1061
HpyF3I CTNAG 3 cut(s) 123, 207, 749
HpySE526I ACGT 3 cut(s) 88, 491, 926
Hsp92I GRCGYC 2 cut(s) 88, 926
Hsp92II CATG 1 cut(s) 458
Kpn2I TCCGGA 2 cut(s) 216, 601
Kzo9I GATC 2 cut(s) 604, 636
LmnI GCTCC 3 cut(s) 583, 737, 787
Lsp1109I GCAGC 4 cut(s) 430, 433, 520, 1064
MaeII ACGT 3 cut(s) 88, 491, 926
MaeIII GTNAC 4 cut(s) 175, 758, 821, 922
MalI GATC 2 cut(s) 606, 638
MboI GATC 2 cut(s) 604, 636
MboII GAAGA 8 cut(s) 275, 278, 307, 335, 409, 606, 609, 853
MfeI CAATTG 1 cut(s) 995
MhlI GDGCHC 1 cut(s) 486
MlsI TGGCCA 1 cut(s) 356
MluCI AATT 8 cut(s) 12, 92, 145, 318, 591, 931, 952, 995
MluNI TGGCCA 1 cut(s) 356
MlyI GAGTC 2 cut(s) 338, 635
MmeI TCCRAC 3 cut(s) 867, 997, 1038
Mox20I TGGCCA 1 cut(s) 356
MroI TCCGGA 2 cut(s) 216, 601
MroXI GAANNNNTTC 1 cut(s) 968
MscI TGGCCA 1 cut(s) 356
MseI TTAA 2 cut(s) 141, 810
MslI CAYNNNNRTG 1 cut(s) 106
Msp20I TGGCCA 1 cut(s) 356
MspA1I CMGCKG 2 cut(s) 424, 503
MspI CCGG 7 cut(s) 156, 217, 389, 461, 602, 708, 908
MspR9I CCNGG 3 cut(s) 461, 709, 777
MunI CAATTG 1 cut(s) 995
Mva1269I GAATGC 2 cut(s) 664, 726
MvaI CCWGG 1 cut(s) 777
MvnI CGCG 2 cut(s) 191, 242
MwoI GCNNNNNNNGC 5 cut(s) 197, 509, 1011, 1020, 1061
NciI CCSGG 2 cut(s) 461, 709
NdeII GATC 2 cut(s) 604, 636
NlaIII CATG 1 cut(s) 458
NlaIV GGNNCC 2 cut(s) 160, 906
NmeAIII GCCGAG 1 cut(s) 472
NmuCI GTSAC 3 cut(s) 175, 758, 922
NspV TTCGAA 1 cut(s) 620
PagI TCATGA 1 cut(s) 454
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PctI GAATGC 2 cut(s) 664, 726
PdmI GAANNNNTTC 1 cut(s) 968
PfeI GAWTC 3 cut(s) 451, 611, 704
Pfl23II CGTACG 1 cut(s) 415
PflFI GACNNNGTC 1 cut(s) 461
PfoI TCCNGGA 1 cut(s) 707
PkrI GCNGC 7 cut(s) 423, 426, 445, 514, 535, 1013, 1054
PleI GAGTC 2 cut(s) 337, 634
PpsI GAGTC 2 cut(s) 337, 634
PshAI GACNNNNGTC 1 cut(s) 778
Psp1406I AACGTT 1 cut(s) 491
Psp6I CCWGG 1 cut(s) 775
PspFI CCCAGC 1 cut(s) 942
PspGI CCWGG 1 cut(s) 775
PspLI CGTACG 1 cut(s) 415
PspN4I GGNNCC 2 cut(s) 160, 906
PspPI GGNCC 1 cut(s) 463
PstNI CAGNNNCTG 1 cut(s) 967
PsyI GACNNNGTC 1 cut(s) 461
RsaI GTAC 4 cut(s) 50, 82, 417, 524
RsaNI GTAC 4 cut(s) 49, 81, 416, 523
RseI CAYNNNNRTG 1 cut(s) 106
Rsr2I CGGWCCG 1 cut(s) 463
RsrII CGGWCCG 1 cut(s) 463
SaqAI TTAA 2 cut(s) 141, 810
SatI GCNGC 7 cut(s) 422, 425, 444, 513, 534, 1012, 1053
Sau3AI GATC 2 cut(s) 604, 636
Sau96I GGNCC 1 cut(s) 463
SchI GAGTC 2 cut(s) 338, 635
ScrFI CCNGG 3 cut(s) 461, 709, 777
SduI GDGCHC 1 cut(s) 486
SfuI TTCGAA 1 cut(s) 620
SinI GGWCC 1 cut(s) 463
SmiMI CAYNNNNRTG 1 cut(s) 106
Sse9I AATT 8 cut(s) 12, 92, 145, 318, 591, 931, 952, 995
SsiI CCGC 6 cut(s) 424, 427, 466, 503, 512, 1011
StyD4I CCNGG 3 cut(s) 459, 707, 775
TaaI ACNGT 2 cut(s) 196, 717
TaiI ACGT 3 cut(s) 91, 494, 929
TaqI TCGA 5 cut(s) 377, 620, 635, 675, 782
TasI AATT 8 cut(s) 12, 92, 145, 318, 591, 931, 952, 995
TatI WGTACW 1 cut(s) 80
TauI GCSGC 3 cut(s) 427, 515, 1014
TfiI GAWTC 3 cut(s) 451, 611, 704
Tru1I TTAA 2 cut(s) 141, 810
Tru9I TTAA 2 cut(s) 141, 810
TscAI CASTG 2 cut(s) 722, 757
TseFI GTSAC 3 cut(s) 175, 758, 922
TseI GCWGC 4 cut(s) 421, 443, 533, 1052
Tsp45I GTSAC 3 cut(s) 175, 758, 922
TspDTI ATGAA 4 cut(s) 157, 443, 810, 911
TspGWI ACGGA 1 cut(s) 245
TspRI CASTG 2 cut(s) 722, 757
Tth111I GACNNNGTC 1 cut(s) 461
VpaK11BI GGWCC 1 cut(s) 463
XapI RAATTY 3 cut(s) 591, 931, 952
XmnI GAANNNNTTC 1 cut(s) 968
ZraI GACGTC 2 cut(s) 89, 927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.