Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0003326

Physical Location & Seq

Genomic Coordinates Forward (+)

22935644 .. 22940010

Gene Structure 4367 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1115200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1248 bp

ATGGAAATCGATTCCCTCCCCAACGGTACCGCCGCGTCGCCGGGACCGACCCTAGGACCCGGCCCCACCGCCGCAACCCCTCCTCCGTCCTCGAAACTGAGCCACCTCGCCGACTCCCTGAAGCTGGAGCACCAGTTCCTCAGAGTCCCCTTCGAGCACTACAAGAAGACGATCCGCGCCAACCACCGCGTCGTGGAGAAGGAGATTTCCTCCGTTATTAACGGCGTCTCCGAAGCAGCTGACCGGGACAACATGTCGTCGGACGACGCCGTGAATCATCTCAGCTCCCTCGTTTCCAGGCTGCAGGGGCTTAAACGAAAGTTGGAAGAGGGGAGTAAGACGGAGCACTTGCAGGCGCAGAGGTGCCGGGCTCGGCTTGATCATTTAGAATCGGCAGATGTGGAGAGTCTGTCTGAATGGAGTAACACACGTGTGAACCGGATCCTTGTGGACTACATGTTGCGGATGTCTTATTATGATACGGCAGTGAAGCTGGCGGAAAGCAGAAATATTCAGGATTTTGTTGATATTGATATATTTCAAGAAGCAAAAAAGGTTATTGAAGCCCTTCAGAATAAGGAGGTAGGCCCTGCCCTAGCCTGGTGTGCCGAGAACAAGTCACGATTAAAGAAATCCAAGAGTAAATTCGAGTTTCAGCTGAGACTTCAAGAGTTTATAGAGTTGGTACGAGCTGAAAATAACTTGCGAGCGATCACATATGCTCAAAAGTACCTAGCACCATGGGGAACTACTCATATGAAAGAATTCCAGCGGGTCTTTGTAACAGTAGCTTATAAGAGTACTACAGAATGTGCTACATACAAGGTTTTATTTGAGCCAAAGCAATGGGATTACCTGGTTGACCAATTCAAACAGGAATTCTGCAAGTTATATGGCATGACGCTTGAGCCTTTGCTGAATATTTATCTGCAAGCAGGCCTGTCTGCTCTTAAAACCCCATACTGTTATGATGATGATTGCACCAAGGAGGATCCGCTATCACAGGAGGGTTTCCGGAAACTAGCCCTGCCCTTACCTTATTCTAAGCAGCATCATTCAAAGCTTGTTTGCTACATAACTAAGGAACTAATGGACACGGAGAACCCACCGCAAGTCTTGCCCAACGGCTATGTCTACAGCACTAAGGCTCTTGAAGAAATGGCGAGGAAGAATGATGGTAAGATTACTTGTCCAAGGACAGGATTGGTATGCAATTTCACAGATCTGGTGAAGGCATATATATCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0019538 GO:0019941 GO:0030163 GO:0032991 GO:0034657 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0051603 GO:0071704 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

CLTH

Protein Analysis

416

Amino Acids

47.26

Weight (kDa)

7.56

Isoelectric Point (pI)

43.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CTLH	PF10607	180 - 321	2.8e-47	CTLH/CRA C-terminal to LisH motif domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003326)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55070 AT3G55070
fragaria_vesca	FvH4_7g20680 FvH4_7g20680 FvH4_7g20680
malus_domestica	MD01G1115200.v1.1 MD01G1115600.v1.1 MD07G1183500.v1.1
prunus_persica	Prupe.2G222200_v2.0.a1 Prupe.2G222200_v2.0.a1 Prupe.2G222200_v2.0.a1
pyrus_communis	pycom01g14420 pycom07g17610
rosa_chinensis	RchiOBHm_Chr1g0364711 RchiOBHm_Chr1g0364821 RchiOBHm_Chr1g0364831
rosa_laevigata	RLG00000027464
rosa_multiflora	Rmu_co8292327.1_g000001 Rmu_sc0028465.1_g000001 Rmu_sc0028466.1_g000001 Rmu_ssc0000112.1_g000032
rosa_roxburghii	Rroxscaffold_4G00291710 Rroxscaffold_4G00291720 Rroxscaffold_4G00292120 Rroxscaffold_4G00292130 Rroxscaffold_4G00292150
rosa_rugosa	Rorug01G0318300 Rorug01G0318500
rosa_samantha	Rh1AG327100 Rh1BG288200 Rh1BG288300 Rh1CG304900 Rh1CG305000 Rh1DG319900
rosa_wichuraiana	Rw1G028970 Rw1G028980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	795
Acc65I	GGTACC	1 cut(s)	26
AccB1I	GGYRCC	2 cut(s)	26, 363
AccI	GTMKAC	1 cut(s)	1134
AccII	CGCG	3 cut(s)	35, 177, 189
AccIII	TCCGGA	1 cut(s)	1014
AclWI	GGATC	5 cut(s)	166, 436, 449, 986, 999
AcsI	RAATTY	3 cut(s)	644, 764, 878
AcuI	CTGAAG	2 cut(s)	140, 554
AcvI	CACGTG	1 cut(s)	431
AcyI	GRCGYC	2 cut(s)	225, 267
AfaI	GTAC	4 cut(s)	28, 687, 731, 802
AfiI	CCNNNNNNNGG	5 cut(s)	53, 124, 193, 600, 1199
AflIII	ACRYGT	4 cut(s)	252, 428, 430, 456
AgsI	TTSAA	6 cut(s)	542, 563, 668, 871, 1059, 1154
AhdI	GACNNNNNGTC	1 cut(s)	253
AjnI	CCWGG	3 cut(s)	296, 599, 855
AjuI	GAANNNNNNNTTGG	2 cut(s)	629, 661
AleI	CACNNNNGTG	1 cut(s)	431
AloI	GAACNNNNNNTCC	2 cut(s)	119, 151
AluBI	AGCT	8 cut(s)	124, 239, 285, 493, 658, 692, 791, 1063
AluI	AGCT	8 cut(s)	124, 239, 285, 493, 658, 692, 791, 1063
Alw21I	GWGCWC	3 cut(s)	132, 159, 348
Alw26I	GTCTC	2 cut(s)	232, 655
AlwI	GGATC	5 cut(s)	166, 436, 449, 986, 999
Aor13HI	TCCGGA	1 cut(s)	1014
AoxI	GGCC	3 cut(s)	61, 586, 937
ApeKI	GCWGC	3 cut(s)	236, 301, 1048
ApoI	RAATTY	3 cut(s)	644, 764, 878
Asp700I	GAANNNNTTC	2 cut(s)	567, 764
Asp718I	GGTACC	1 cut(s)	26
AspA2I	CCTAGG	1 cut(s)	52
AspLEI	GCGC	2 cut(s)	179, 358
AspS9I	GGNCC	4 cut(s)	44, 56, 62, 587
AsuC2I	CCSGG	4 cut(s)	42, 60, 245, 368
AsuHPI	GGTGA	1 cut(s)	1240
AvaII	GGWCC	2 cut(s)	44, 56
AvrII	CCTAGG	1 cut(s)	52
BaeI	ACNNNNGTAYC	2 cut(s)	471, 504
BamHI	GGATCC	2 cut(s)	441, 991
BanI	GGYRCC	2 cut(s)	26, 363
BanII	GRGCYC	1 cut(s)	373
BbrPI	CACGTG	1 cut(s)	431
BbsI	GAAGAC	1 cut(s)	173
Bbv12I	GWGCWC	3 cut(s)	132, 159, 348
BbvI	GCAGC	3 cut(s)	248, 288, 1060
BccI	CCATC	1 cut(s)	1169
BceAI	ACGGC	4 cut(s)	238, 254, 498, 1141
BciT130I	CCWGG	3 cut(s)	298, 601, 857
BclI	TGATCA	1 cut(s)	379
BcnI	CCSGG	4 cut(s)	42, 60, 245, 368
BcoDI	GTCTC	2 cut(s)	232, 655
BfaI	CTAG	4 cut(s)	53, 596, 734, 1022
BfmI	CTRYAG	3 cut(s)	302, 804, 1135
BglII	AGATCT	1 cut(s)	1222
BisI	GCNGC	5 cut(s)	33, 72, 237, 302, 1049
BlnI	CCTAGG	1 cut(s)	52
BlsI	GCNGC	5 cut(s)	34, 73, 238, 303, 1050
BmcAI	AGTACT	1 cut(s)	802
Bme1390I	CCNGG	7 cut(s)	42, 60, 245, 298, 368, 601, 857
Bme18I	GGWCC	2 cut(s)	44, 56
BmeRI	GACNNNNNGTC	1 cut(s)	253
BmgT120I	GGNCC	4 cut(s)	44, 56, 62, 587
BmiI	GGNNCC	7 cut(s)	28, 45, 58, 64, 365, 443, 993
BmrFI	CCNGG	7 cut(s)	42, 60, 245, 298, 368, 601, 857
BmsI	GCATC	1 cut(s)	1060
BpiI	GAAGAC	1 cut(s)	173
BplI	GAGNNNNNCTC	2 cut(s)	194, 226
BpmI	CTGGAG	1 cut(s)	146
BpuEI	CTTGAG	1 cut(s)	926
BpuMI	CCSGG	4 cut(s)	42, 60, 245, 368
Bsa29I	ATCGAT	1 cut(s)	9
BsaAI	YACGTR	1 cut(s)	431
BsaHI	GRCGYC	2 cut(s)	225, 267
BsaJI	CCNNGG	4 cut(s)	52, 740, 984, 1193
BsaWI	WCCGGW	2 cut(s)	438, 1014
BsaXI	ACNNNNNCTCC	8 cut(s)	67, 97, 119, 149, 212, 242, 395, 425
Bsc4I	CCNNNNNNNGG	5 cut(s)	53, 124, 193, 600, 1199
Bse1I	ACTGG	1 cut(s)	133
Bse3DI	GCAATG	1 cut(s)	851
BseAI	TCCGGA	1 cut(s)	1014
BseBI	CCWGG	3 cut(s)	298, 601, 857
BseCI	ATCGAT	1 cut(s)	9
BseDI	CCNNGG	4 cut(s)	52, 740, 984, 1193
BseGI	GGATG	1 cut(s)	471
BseLI	CCNNNNNNNGG	5 cut(s)	53, 124, 193, 600, 1199
BseMI	GCAATG	1 cut(s)	851
BseMII	CTCAG	4 cut(s)	89, 154, 295, 650
BseNI	ACTGG	1 cut(s)	133
BseRI	GAGGAG	1 cut(s)	72
BseXI	GCAGC	3 cut(s)	248, 288, 1060
Bsh1236I	CGCG	3 cut(s)	35, 177, 189
BshFI	GGCC	3 cut(s)	63, 588, 939
BshNI	GGYRCC	2 cut(s)	26, 363
BshVI	ATCGAT	1 cut(s)	9
BsiHKAI	GWGCWC	3 cut(s)	132, 159, 348
BsiSI	CCGG	6 cut(s)	41, 60, 244, 367, 439, 1015
BslFI	GGGAC	3 cut(s)	57, 131, 260
BslI	CCNNNNNNNGG	5 cut(s)	53, 124, 193, 600, 1199
BsmAI	GTCTC	2 cut(s)	232, 655
BsmBI	CGTCTC	1 cut(s)	232
BsmFI	GGGAC	3 cut(s)	57, 131, 260
BsnI	GGCC	3 cut(s)	63, 588, 939
Bsp1286I	GDGCHC	4 cut(s)	132, 159, 348, 373
Bsp13I	TCCGGA	1 cut(s)	1014
Bsp143I	GATC	6 cut(s)	171, 379, 441, 711, 991, 1222
Bsp19I	CCATGG	1 cut(s)	740
BspANI	GGCC	3 cut(s)	63, 588, 939
BspCNI	CTCAG	4 cut(s)	90, 153, 294, 651
BspDI	ATCGAT	1 cut(s)	9
BspEI	TCCGGA	1 cut(s)	1014
BspFNI	CGCG	3 cut(s)	35, 177, 189
BspHI	TCATGA	1 cut(s)	1244
BspLI	GGNNCC	7 cut(s)	28, 45, 58, 64, 365, 443, 993
BspMAI	CTGCAG	1 cut(s)	306
BspPI	GGATC	5 cut(s)	166, 436, 449, 986, 999
BspT107I	GGYRCC	2 cut(s)	26, 363
BsrDI	GCAATG	1 cut(s)	851
BsrI	ACTGG	1 cut(s)	133
BssECI	CCNNGG	4 cut(s)	52, 740, 984, 1193
BssMI	GATC	6 cut(s)	171, 379, 441, 711, 991, 1222
BssNI	GRCGYC	2 cut(s)	225, 267
BssT1I	CCWWGG	4 cut(s)	52, 740, 984, 1193
Bst2UI	CCWGG	3 cut(s)	298, 601, 857
Bst4CI	ACNGT	3 cut(s)	26, 787, 965
Bst6I	CTCTTC	1 cut(s)	321
BstACI	GRCGYC	2 cut(s)	225, 267
BstAPI	GCANNNNNTGC	1 cut(s)	1117
BstBAI	YACGTR	1 cut(s)	431
BstC8I	GCNNGC	5 cut(s)	354, 495, 708, 933, 937
BstDEI	CTNAG	7 cut(s)	98, 140, 281, 659, 1044, 1080, 1143
BstDSI	CCRYGG	1 cut(s)	740
BstF5I	GGATG	1 cut(s)	471
BstFNI	CGCG	3 cut(s)	35, 177, 189
BstHHI	GCGC	2 cut(s)	179, 358
BstKTI	GATC	6 cut(s)	174, 382, 444, 714, 994, 1225
BstMAI	GTCTC	2 cut(s)	232, 655
BstMBI	GATC	6 cut(s)	171, 379, 441, 711, 991, 1222
BstMWI	GCNNNNNNNGC	3 cut(s)	307, 605, 1117
BstNI	CCWGG	3 cut(s)	298, 601, 857
BstNSI	RCATGY	2 cut(s)	256, 460
BstSCI	CCNGG	7 cut(s)	40, 58, 243, 296, 366, 599, 855
BstSFI	CTRYAG	3 cut(s)	302, 804, 1135
BstUI	CGCG	3 cut(s)	35, 177, 189
BstV1I	GCAGC	3 cut(s)	248, 288, 1060
BstV2I	GAAGAC	1 cut(s)	173
BstX2I	RGATCY	3 cut(s)	441, 991, 1222
BstXI	CCANNNNNNTGG	1 cut(s)	846
BstYI	RGATCY	3 cut(s)	441, 991, 1222
Bsu15I	ATCGAT	1 cut(s)	9
BsuRI	GGCC	3 cut(s)	63, 588, 939
BsuTUI	ATCGAT	1 cut(s)	9
BtgI	CCRYGG	1 cut(s)	740
BtsCI	GGATG	1 cut(s)	471
BtsI	GCAGTG	1 cut(s)	492
BtsIMutI	CAGTG	1 cut(s)	492
Cac8I	GCNNGC	5 cut(s)	354, 495, 708, 933, 937
CciI	TCATGA	1 cut(s)	1244
CfoI	GCGC	2 cut(s)	179, 358
Cfr13I	GGNCC	4 cut(s)	44, 56, 62, 587
ClaI	ATCGAT	1 cut(s)	9
CseI	GACGC	5 cut(s)	24, 178, 214, 275, 910
CsiI	ACCWGGT	1 cut(s)	855
Csp6I	GTAC	4 cut(s)	27, 686, 730, 801
CviAII	CATG	5 cut(s)	253, 457, 741, 898, 1245
CviQI	GTAC	4 cut(s)	27, 686, 730, 801
DdeI	CTNAG	7 cut(s)	98, 140, 281, 659, 1044, 1080, 1143
DpnI	GATC	6 cut(s)	173, 381, 443, 713, 993, 1224
DpnII	GATC	6 cut(s)	171, 379, 441, 711, 991, 1222
DriI	GACNNNNNGTC	1 cut(s)	253
Eam1104I	CTCTTC	1 cut(s)	321
Eam1105I	GACNNNNNGTC	1 cut(s)	253
EarI	CTCTTC	1 cut(s)	321
EciI	GGCGGA	1 cut(s)	512
Eco130I	CCWWGG	4 cut(s)	52, 740, 984, 1193
Eco147I	AGGCCT	1 cut(s)	939
Eco24I	GRGCYC	1 cut(s)	373
Eco47I	GGWCC	2 cut(s)	44, 56
Eco57I	CTGAAG	2 cut(s)	140, 554
Eco72I	CACGTG	1 cut(s)	431
EcoO109I	RGGNCCY	2 cut(s)	56, 587
EcoRI	GAATTC	2 cut(s)	764, 878
EcoRII	CCWGG	3 cut(s)	296, 599, 855
EcoT14I	CCWWGG	4 cut(s)	52, 740, 984, 1193
EcoT38I	GRGCYC	1 cut(s)	373
ErhI	CCWWGG	4 cut(s)	52, 740, 984, 1193
Esp3I	CGTCTC	1 cut(s)	232
FaeI	CATG	5 cut(s)	256, 460, 744, 901, 1248
FaqI	GGGAC	3 cut(s)	57, 131, 260
FatI	CATG	5 cut(s)	252, 456, 740, 897, 1244
FauI	CCCGC	1 cut(s)	765
FauNDI	CATATG	2 cut(s)	718, 756
FbaI	TGATCA	1 cut(s)	379
FblI	GTMKAC	1 cut(s)	1134
Fnu4HI	GCNGC	5 cut(s)	33, 72, 237, 302, 1049
FokI	GGATG	1 cut(s)	478
FriOI	GRGCYC	1 cut(s)	373
Fsp4HI	GCNGC	5 cut(s)	33, 72, 237, 302, 1049
FspBI	CTAG	4 cut(s)	53, 596, 734, 1022
GlaI	GCGC	2 cut(s)	178, 357
GluI	GCNGC	5 cut(s)	33, 72, 237, 302, 1049
GsuI	CTGGAG	1 cut(s)	146
HaeIII	GGCC	3 cut(s)	63, 588, 939
HapII	CCGG	6 cut(s)	41, 60, 244, 367, 439, 1015
HgaI	GACGC	5 cut(s)	24, 178, 214, 275, 910
HhaI	GCGC	2 cut(s)	179, 358
Hin1I	GRCGYC	2 cut(s)	225, 267
Hin1II	CATG	5 cut(s)	256, 460, 744, 901, 1248
Hin6I	GCGC	2 cut(s)	177, 356
HinP1I	GCGC	2 cut(s)	177, 356
HincII	GTYRAC	1 cut(s)	862
HindII	GTYRAC	1 cut(s)	862
HindIII	AAGCTT	1 cut(s)	1061
HinfI	GANTC	6 cut(s)	11, 113, 144, 274, 389, 406
HpaII	CCGG	6 cut(s)	41, 60, 244, 367, 439, 1015
HphI	GGTGA	1 cut(s)	1240
Hpy166II	GTNNAC	4 cut(s)	436, 451, 862, 1135
Hpy188I	TCNGA	5 cut(s)	143, 232, 262, 415, 573
Hpy188III	TCNNGA	7 cut(s)	515, 542, 621, 668, 1015, 1151, 1245
Hpy8I	GTNNAC	4 cut(s)	436, 451, 862, 1135
Hpy99I	CGWCG	4 cut(s)	40, 194, 262, 269
HpyAV	CCTTC	4 cut(s)	160, 193, 578, 1225
HpyCH4III	ACNGT	3 cut(s)	26, 787, 965
HpyCH4IV	ACGT	1 cut(s)	430
HpyCH4V	TGCA	6 cut(s)	304, 352, 885, 931, 981, 1212
HpyF10VI	GCNNNNNNNGC	3 cut(s)	307, 605, 1117
HpyF3I	CTNAG	7 cut(s)	98, 140, 281, 659, 1044, 1080, 1143
HpySE526I	ACGT	1 cut(s)	430
Hsp92I	GRCGYC	2 cut(s)	225, 267
Hsp92II	CATG	5 cut(s)	256, 460, 744, 901, 1248
HspAI	GCGC	2 cut(s)	177, 356
Kpn2I	TCCGGA	1 cut(s)	1014
KpnI	GGTACC	1 cut(s)	30
Ksp22I	TGATCA	1 cut(s)	379
Kzo9I	GATC	6 cut(s)	171, 379, 441, 711, 991, 1222
LmnI	GCTCC	3 cut(s)	127, 290, 343
Lsp1109I	GCAGC	3 cut(s)	248, 288, 1060
LweI	GCATC	1 cut(s)	1060
MabI	ACCWGGT	1 cut(s)	855
MaeI	CTAG	4 cut(s)	53, 596, 734, 1022
MaeII	ACGT	1 cut(s)	430
MaeIII	GTNAC	3 cut(s)	422, 618, 781
MalI	GATC	6 cut(s)	173, 381, 443, 713, 993, 1224
MboI	GATC	6 cut(s)	171, 379, 441, 711, 991, 1222
MboII	GAAGA	4 cut(s)	178, 338, 1166, 1180
MflI	RGATCY	3 cut(s)	441, 991, 1222
MhlI	GDGCHC	4 cut(s)	132, 159, 348, 373
MluCI	AATT	5 cut(s)	644, 764, 866, 878, 1213
MlyI	GAGTC	3 cut(s)	107, 153, 415
MmeI	TCCRAC	2 cut(s)	240, 303
MroI	TCCGGA	1 cut(s)	1014
MroXI	GAANNNNTTC	2 cut(s)	567, 764
MseI	TTAA	4 cut(s)	219, 312, 626, 951
MslI	CAYNNNNRTG	1 cut(s)	431
MspA1I	CMGCKG	3 cut(s)	239, 658, 772
MspI	CCGG	6 cut(s)	41, 60, 244, 367, 439, 1015
MspR9I	CCNGG	7 cut(s)	42, 60, 245, 298, 368, 601, 857
MvaI	CCWGG	3 cut(s)	298, 601, 857
MvnI	CGCG	3 cut(s)	35, 177, 189
MwoI	GCNNNNNNNGC	3 cut(s)	307, 605, 1117
NciI	CCSGG	4 cut(s)	42, 60, 245, 368
NcoI	CCATGG	1 cut(s)	740
NdeI	CATATG	2 cut(s)	718, 756
NdeII	GATC	6 cut(s)	171, 379, 441, 711, 991, 1222
NlaIII	CATG	5 cut(s)	256, 460, 744, 901, 1248
NlaIV	GGNNCC	7 cut(s)	28, 45, 58, 64, 365, 443, 993
NmeAIII	GCCGAG	2 cut(s)	352, 634
NmuCI	GTSAC	1 cut(s)	618
NspI	RCATGY	2 cut(s)	256, 460
OliI	CACNNNNGTG	1 cut(s)	431
PagI	TCATGA	1 cut(s)	1244
PceI	AGGCCT	1 cut(s)	939
PciI	ACATGT	2 cut(s)	252, 456
PcsI	WCGNNNNNNNCGW	2 cut(s)	44, 228
PdmI	GAANNNNTTC	2 cut(s)	567, 764
PfeI	GAWTC	3 cut(s)	11, 274, 389
PkrI	GCNGC	5 cut(s)	34, 73, 238, 303, 1050
PleI	GAGTC	3 cut(s)	107, 152, 414
PmaCI	CACGTG	1 cut(s)	431
PmlI	CACGTG	1 cut(s)	431
PpsI	GAGTC	3 cut(s)	107, 152, 414
Ppu21I	YACGTR	1 cut(s)	431
PpuMI	RGGWCCY	1 cut(s)	56
PscI	ACATGT	2 cut(s)	252, 456
PsiI	TTATAA	1 cut(s)	795
Psp5II	RGGWCCY	1 cut(s)	56
Psp6I	CCWGG	3 cut(s)	296, 599, 855
PspCI	CACGTG	1 cut(s)	431
PspGI	CCWGG	3 cut(s)	296, 599, 855
PspN4I	GGNNCC	7 cut(s)	28, 45, 58, 64, 365, 443, 993
PspPI	GGNCC	4 cut(s)	44, 56, 62, 587
PspPPI	RGGWCCY	1 cut(s)	56
PstI	CTGCAG	1 cut(s)	306
PsuI	RGATCY	3 cut(s)	441, 991, 1222
PvuII	CAGCTG	2 cut(s)	239, 658
RsaI	GTAC	4 cut(s)	28, 687, 731, 802
RsaNI	GTAC	4 cut(s)	27, 686, 730, 801
RseI	CAYNNNNRTG	1 cut(s)	431
SaqAI	TTAA	4 cut(s)	219, 312, 626, 951
SatI	GCNGC	5 cut(s)	33, 72, 237, 302, 1049
Sau3AI	GATC	6 cut(s)	171, 379, 441, 711, 991, 1222
Sau96I	GGNCC	4 cut(s)	44, 56, 62, 587
ScaI	AGTACT	1 cut(s)	802
SchI	GAGTC	3 cut(s)	107, 153, 415
ScrFI	CCNGG	7 cut(s)	42, 60, 245, 298, 368, 601, 857
SduI	GDGCHC	4 cut(s)	132, 159, 348, 373
SexAI	ACCWGGT	1 cut(s)	855
SfaNI	GCATC	1 cut(s)	1060
SfcI	CTRYAG	3 cut(s)	302, 804, 1135
SinI	GGWCC	2 cut(s)	44, 56
SmiMI	CAYNNNNRTG	1 cut(s)	431
SmlI	CTYRAG	1 cut(s)	905
SmoI	CTYRAG	1 cut(s)	905
Sse9I	AATT	5 cut(s)	644, 764, 866, 878, 1213
SseBI	AGGCCT	1 cut(s)	939
SspI	AATATT	2 cut(s)	511, 922
SspMI	CTAG	4 cut(s)	53, 596, 734, 1022
StuI	AGGCCT	1 cut(s)	939
StyD4I	CCNGG	7 cut(s)	40, 58, 243, 296, 366, 599, 855
StyI	CCWWGG	4 cut(s)	52, 740, 984, 1193
TaaI	ACNGT	3 cut(s)	26, 787, 965
TaiI	ACGT	1 cut(s)	433
TaqI	TCGA	4 cut(s)	9, 92, 153, 648
TaqII	GACCGA	1 cut(s)	61
TasI	AATT	5 cut(s)	644, 764, 866, 878, 1213
TatI	WGTACW	1 cut(s)	800
TauI	GCSGC	2 cut(s)	35, 74
TfiI	GAWTC	3 cut(s)	11, 274, 389
Tru1I	TTAA	4 cut(s)	219, 312, 626, 951
Tru9I	TTAA	4 cut(s)	219, 312, 626, 951
TscAI	CASTG	1 cut(s)	492
TseFI	GTSAC	1 cut(s)	618
TseI	GCWGC	3 cut(s)	236, 301, 1048
Tsp45I	GTSAC	1 cut(s)	618
TspDTI	ATGAA	1 cut(s)	773
TspGWI	ACGGA	4 cut(s)	75, 202, 356, 1112
TspRI	CASTG	1 cut(s)	492
VpaK11BI	GGWCC	2 cut(s)	44, 56
XapI	RAATTY	3 cut(s)	644, 764, 878
XceI	RCATGY	2 cut(s)	256, 460
XmaJI	CCTAGG	1 cut(s)	52
XmiI	GTMKAC	1 cut(s)	1134
XmnI	GAANNNNTTC	2 cut(s)	567, 764
XspI	CTAG	4 cut(s)	53, 596, 734, 1022
ZrmI	AGTACT	1 cut(s)	802

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.