MD01G1115600.v1.1

Macrophage erythroblast

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003326
Physical Location & Seq
Forward (+)
22950360 .. 22954264
3905 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1115600.v1.1.491

Sequence Viewer

Length: 1479 bp
ATGGAAATTGATTCTCTCCCCAAAGGCACCGCCGCGTCGCCAACGACAGCTTGTCAAGATCAAAGAACAAAAATTAACAAGGGGCGTGAGAAAAGTAAAAATGGATGTGTGGATAGCACACAACTTATTTTATCCTGTTTGGATGACGGAGGAGTGAGAGGAAATAGAAAAGAACAGAGGTTAATCCGATGGATTCAAGGCAACCAAAAAGAGTTGGAAGAGGGGAGTAAGACGGAGCACTTGCAGGCACAGAGGTGCCGGGCTCGGCTTGATCATTTAGAATCGGCAGATGTGGAGAGTCTGTCTGAATGGAGTAACACACGTGTGAACCGGATCCTTGTGGACTACATGTTGCGGATGTCTTATTATGATACGGCAGTGAAGCTGGCGGAAAGCAGAAATATTCAGGTAGTTTTGGTTTCCTGTCCTAGATTTGTTGACATTACATATTTGTGGTTCTTTATACCGAGTGCTCTGTCATCAGTTTTTTTCATTCCAATGTTCATAAATTCATGGAAGGATTTTGTTGATATTGATATATTTCAAGAAGCAAAAAAGGTTATTGAAGCCCTTCAGAATATGGAGGTAGGCCCTGCCCTAGCCTGGTGTGCCGAGAACAAGTCACGATTAAAGAAATCCAAGAGTAAATTCGAGTTTCAGCTGAGACTTCAAGAGTTTATAGAGTTGGTACGAGCTGAAAATAACTTGCGAGCAATCACATATGCTCAAAAGTACCTAGCACCATGGGGAACTACTCATATGAAAGAATTCCAGCGGGTCTTTGTAACAGTAGCTTATAAGAGTACTACAGAATGTGCTACATACAAGGTTTTATTTGAGCCAAAGCAATGGGATTACCTGGTTGACCAATTCAAACAGGAATTCTGCAAGTTATATGGCATGACGCTTGAGCCTTTGCTGAATATTTATCTGCAAGCAGGCCTGTCTGCTCTTAAAACCCCGCATCCTCTAACAGAGTGTTCTAGGCTTGGTACAATCGAGTTCACTTTATATGGAGTTGACTCCCAAAGCTTCTTTGGCTTTGAGCTGCATCGTTGCCTATGTGACCTGCAGAACAAAACTCTAGAGCGGGAGGGTGAAACAGCTATAGCGTATGTACATACTTATGTGATTGAAATAGTGAACCAAAATTTTTGTATATGGAATGAAGTTTTGACAATGCGTACTGTATACTGTTATGATGATGATTGCACCAAGGAGGATCCGCTATCACAGGAGGGTTTCCGGAAACTAGCCATGCCCTTACCTTATTCTAAGCAGCGTCATTCAAAGCTTGTTTGCTACATAACTAAGGAACTAATGGACACAGAGAACCCACCGCAAGTCTTGCCCAACGGCTATGTCTACAGCACTAAGGCTCTTGAAGAAATGGCGAGGAAGAATGATGGTAAGATTACTTGTCCAAGGACAGGATTGGTATGCAATTTCACAGATCTGGTGAAGGCATATATATCATGA

Protein Analysis

493

Amino Acids

56.83

Weight (kDa)

7.01

Isoelectric Point (pI)

42.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CTLH PF10607 181 - 321 2.7e-46 CTLH/CRA C-terminal to LisH motif domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 798
Acc36I ACCTGC 1 cut(s) 1077
AccB1I GGYRCC 2 cut(s) 26, 255
AccBSI CCGCTC 1 cut(s) 1090
AccI GTMKAC 2 cut(s) 1191, 1365
AccII CGCG 1 cut(s) 35
AccIII TCCGGA 1 cut(s) 1245
AciI CCGC 9 cut(s) 30, 33, 355, 389, 775, 962, 1090, 1226, 1340
AclWI GGATC 4 cut(s) 328, 341, 1217, 1230
AcsI RAATTY 5 cut(s) 508, 647, 767, 881, 1150
AcuI CTGAAG 1 cut(s) 557
AcvI CACGTG 1 cut(s) 323
AfaI GTAC 6 cut(s) 690, 734, 805, 994, 1119, 1186
AfiI CCNNNNNNNGG 2 cut(s) 603, 1430
AflIII ACRYGT 3 cut(s) 320, 322, 348
AgsI TTSAA 8 cut(s) 197, 545, 566, 671, 874, 1136, 1290, 1385
AhdI GACNNNNNGTC 1 cut(s) 51
AjnI CCWGG 2 cut(s) 602, 858
AjuI GAANNNNNNNTTGG 2 cut(s) 632, 664
AleI CACNNNNGTG 2 cut(s) 253, 323
AluBI AGCT 9 cut(s) 50, 385, 661, 695, 794, 1032, 1048, 1106, 1294
AluI AGCT 9 cut(s) 50, 385, 661, 695, 794, 1032, 1048, 1106, 1294
Alw21I GWGCWC 2 cut(s) 240, 475
Alw26I GTCTC 1 cut(s) 658
AlwI GGATC 4 cut(s) 328, 341, 1217, 1230
Aor13HI TCCGGA 1 cut(s) 1245
AoxI GGCC 2 cut(s) 589, 940
ApeKI GCWGC 2 cut(s) 1048, 1279
ApoI RAATTY 5 cut(s) 508, 647, 767, 881, 1150
Asp700I GAANNNNTTC 2 cut(s) 570, 767
AspS9I GGNCC 1 cut(s) 590
AsuC2I CCSGG 1 cut(s) 260
AsuHPI GGTGA 2 cut(s) 1109, 1471
BaeI ACNNNNGTAYC 2 cut(s) 363, 396
BamHI GGATCC 2 cut(s) 333, 1222
BanI GGYRCC 2 cut(s) 26, 255
BanII GRGCYC 1 cut(s) 265
BbrPI CACGTG 1 cut(s) 323
Bbv12I GWGCWC 2 cut(s) 240, 475
BbvI GCAGC 2 cut(s) 1035, 1291
BccI CCATC 2 cut(s) 183, 1400
BceAI ACGGC 2 cut(s) 390, 1372
BciT130I CCWGG 2 cut(s) 604, 860
BclI TGATCA 1 cut(s) 271
BcnI CCSGG 1 cut(s) 260
BcoDI GTCTC 1 cut(s) 658
BfaI CTAG 6 cut(s) 429, 599, 737, 984, 1085, 1253
BfmI CTRYAG 4 cut(s) 807, 1070, 1107, 1366
BfuAI ACCTGC 1 cut(s) 1077
BglII AGATCT 1 cut(s) 1453
BisI GCNGC 3 cut(s) 33, 1049, 1280
BlsI GCNGC 3 cut(s) 34, 1050, 1281
BmcAI AGTACT 1 cut(s) 805
Bme1390I CCNGG 3 cut(s) 260, 604, 860
BmeRI GACNNNNNGTC 1 cut(s) 51
BmgT120I GGNCC 1 cut(s) 590
BmiI GGNNCC 4 cut(s) 28, 257, 335, 1224
BmrFI CCNGG 3 cut(s) 260, 604, 860
BmsI GCATC 2 cut(s) 973, 1060
BpuEI CTTGAG 1 cut(s) 929
BpuMI CCSGG 1 cut(s) 260
BsaAI YACGTR 1 cut(s) 323
BsaJI CCNNGG 3 cut(s) 743, 1215, 1424
BsaWI WCCGGW 2 cut(s) 330, 1245
BsaXI ACNNNNNCTCC 2 cut(s) 287, 317
Bsc4I CCNNNNNNNGG 2 cut(s) 603, 1430
Bse3DI GCAATG 1 cut(s) 854
BseAI TCCGGA 1 cut(s) 1245
BseBI CCWGG 2 cut(s) 604, 860
BseDI CCNNGG 3 cut(s) 743, 1215, 1424
BseGI GGATG 4 cut(s) 110, 148, 363, 964
BseLI CCNNNNNNNGG 2 cut(s) 603, 1430
BseMI GCAATG 1 cut(s) 854
BseMII CTCAG 1 cut(s) 653
BseRI GAGGAG 1 cut(s) 165
BseXI GCAGC 2 cut(s) 1035, 1291
Bsh1236I CGCG 1 cut(s) 35
BshFI GGCC 2 cut(s) 591, 942
BshNI GGYRCC 2 cut(s) 26, 255
BsiHKAI GWGCWC 2 cut(s) 240, 475
BsiSI CCGG 3 cut(s) 259, 331, 1246
BslI CCNNNNNNNGG 2 cut(s) 603, 1430
BsmAI GTCTC 1 cut(s) 658
BsnI GGCC 2 cut(s) 591, 942
Bsp1286I GDGCHC 3 cut(s) 240, 265, 475
Bsp13I TCCGGA 1 cut(s) 1245
Bsp1407I TGTACA 1 cut(s) 1117
Bsp143I GATC 5 cut(s) 58, 271, 333, 1222, 1453
Bsp19I CCATGG 1 cut(s) 743
BspACI CCGC 9 cut(s) 30, 33, 355, 389, 775, 962, 1090, 1226, 1340
BspANI GGCC 2 cut(s) 591, 942
BspCNI CTCAG 1 cut(s) 654
BspEI TCCGGA 1 cut(s) 1245
BspFNI CGCG 1 cut(s) 35
BspHI TCATGA 1 cut(s) 1475
BspLI GGNNCC 4 cut(s) 28, 257, 335, 1224
BspMAI CTGCAG 1 cut(s) 1074
BspMI ACCTGC 1 cut(s) 1077
BspPI GGATC 4 cut(s) 328, 341, 1217, 1230
BspT107I GGYRCC 2 cut(s) 26, 255
BsrBI CCGCTC 1 cut(s) 1090
BsrDI GCAATG 1 cut(s) 854
BsrGI TGTACA 1 cut(s) 1117
BssECI CCNNGG 3 cut(s) 743, 1215, 1424
BssMI GATC 5 cut(s) 58, 271, 333, 1222, 1453
BssNAI GTATAC 1 cut(s) 1192
BssT1I CCWWGG 3 cut(s) 743, 1215, 1424
Bst1107I GTATAC 1 cut(s) 1192
Bst2UI CCWGG 2 cut(s) 604, 860
Bst4CI ACNGT 3 cut(s) 790, 1189, 1196
Bst6I CTCTTC 1 cut(s) 213
BstAPI GCANNNNNTGC 1 cut(s) 1348
BstAUI TGTACA 1 cut(s) 1117
BstBAI YACGTR 1 cut(s) 323
BstC8I GCNNGC 5 cut(s) 246, 387, 711, 936, 940
BstDEI CTNAG 4 cut(s) 662, 1275, 1311, 1374
BstDSI CCRYGG 1 cut(s) 743
BstF5I GGATG 4 cut(s) 110, 148, 363, 964
BstFNI CGCG 1 cut(s) 35
BstKTI GATC 5 cut(s) 61, 274, 336, 1225, 1456
BstMAI GTCTC 1 cut(s) 658
BstMBI GATC 5 cut(s) 58, 271, 333, 1222, 1453
BstMWI GCNNNNNNNGC 3 cut(s) 608, 1038, 1348
BstNI CCWGG 2 cut(s) 604, 860
BstNSI RCATGY 1 cut(s) 352
BstSCI CCNGG 3 cut(s) 258, 602, 858
BstSFI CTRYAG 4 cut(s) 807, 1070, 1107, 1366
BstUI CGCG 1 cut(s) 35
BstV1I GCAGC 2 cut(s) 1035, 1291
BstX2I RGATCY 3 cut(s) 333, 1222, 1453
BstXI CCANNNNNNTGG 1 cut(s) 849
BstYI RGATCY 3 cut(s) 333, 1222, 1453
BstZ17I GTATAC 1 cut(s) 1192
BsuRI GGCC 2 cut(s) 591, 942
BtgI CCRYGG 1 cut(s) 743
BtsCI GGATG 4 cut(s) 110, 148, 363, 964
BtsI GCAGTG 1 cut(s) 384
BtsIMutI CAGTG 1 cut(s) 384
BveI ACCTGC 1 cut(s) 1077
Cac8I GCNNGC 5 cut(s) 246, 387, 711, 936, 940
CciI TCATGA 1 cut(s) 1475
Cfr13I GGNCC 1 cut(s) 590
CseI GACGC 3 cut(s) 24, 913, 1271
CsiI ACCWGGT 1 cut(s) 858
Csp6I GTAC 6 cut(s) 689, 733, 804, 993, 1118, 1185
CviAII CATG 6 cut(s) 349, 513, 744, 901, 1258, 1476
CviQI GTAC 6 cut(s) 689, 733, 804, 993, 1118, 1185
DdeI CTNAG 4 cut(s) 662, 1275, 1311, 1374
DpnI GATC 5 cut(s) 60, 273, 335, 1224, 1455
DpnII GATC 5 cut(s) 58, 271, 333, 1222, 1453
DriI GACNNNNNGTC 1 cut(s) 51
Eam1104I CTCTTC 1 cut(s) 213
Eam1105I GACNNNNNGTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 213
EciI GGCGGA 1 cut(s) 404
Eco130I CCWWGG 3 cut(s) 743, 1215, 1424
Eco147I AGGCCT 1 cut(s) 942
Eco24I GRGCYC 1 cut(s) 265
Eco57I CTGAAG 1 cut(s) 557
Eco72I CACGTG 1 cut(s) 323
EcoO109I RGGNCCY 1 cut(s) 590
EcoRI GAATTC 2 cut(s) 767, 881
EcoRII CCWGG 2 cut(s) 602, 858
EcoT14I CCWWGG 3 cut(s) 743, 1215, 1424
EcoT38I GRGCYC 1 cut(s) 265
ErhI CCWWGG 3 cut(s) 743, 1215, 1424
FaeI CATG 6 cut(s) 352, 516, 747, 904, 1261, 1479
FatI CATG 6 cut(s) 348, 512, 743, 900, 1257, 1475
FauI CCCGC 3 cut(s) 768, 969, 1083
FauNDI CATATG 2 cut(s) 721, 759
FbaI TGATCA 1 cut(s) 271
FblI GTMKAC 2 cut(s) 1191, 1365
Fnu4HI GCNGC 3 cut(s) 33, 1049, 1280
FokI GGATG 4 cut(s) 117, 155, 370, 951
FriOI GRGCYC 1 cut(s) 265
Fsp4HI GCNGC 3 cut(s) 33, 1049, 1280
FspBI CTAG 6 cut(s) 429, 599, 737, 984, 1085, 1253
GluI GCNGC 3 cut(s) 33, 1049, 1280
HaeIII GGCC 2 cut(s) 591, 942
HapII CCGG 3 cut(s) 259, 331, 1246
HgaI GACGC 3 cut(s) 24, 913, 1271
Hin1II CATG 6 cut(s) 352, 516, 747, 904, 1261, 1479
HincII GTYRAC 3 cut(s) 439, 865, 1021
HindII GTYRAC 3 cut(s) 439, 865, 1021
HindIII AAGCTT 2 cut(s) 1030, 1292
HinfI GANTC 5 cut(s) 11, 193, 281, 298, 1022
HpaII CCGG 3 cut(s) 259, 331, 1246
HphI GGTGA 2 cut(s) 1109, 1471
Hpy166II GTNNAC 9 cut(s) 328, 343, 439, 865, 1005, 1021, 1144, 1192, 1366
Hpy188I TCNGA 3 cut(s) 188, 307, 576
Hpy188III TCNNGA 8 cut(s) 56, 545, 624, 671, 1085, 1246, 1382, 1476
Hpy8I GTNNAC 9 cut(s) 328, 343, 439, 865, 1005, 1021, 1144, 1192, 1366
Hpy99I CGWCG 1 cut(s) 40
HpyAV CCTTC 3 cut(s) 511, 581, 1456
HpyCH4III ACNGT 3 cut(s) 790, 1189, 1196
HpyCH4IV ACGT 1 cut(s) 322
HpyCH4V TGCA 7 cut(s) 244, 888, 934, 1051, 1072, 1212, 1443
HpyF10VI GCNNNNNNNGC 3 cut(s) 608, 1038, 1348
HpyF3I CTNAG 4 cut(s) 662, 1275, 1311, 1374
HpySE526I ACGT 1 cut(s) 322
Hsp92II CATG 6 cut(s) 352, 516, 747, 904, 1261, 1479
Kpn2I TCCGGA 1 cut(s) 1245
Ksp22I TGATCA 1 cut(s) 271
Kzo9I GATC 5 cut(s) 58, 271, 333, 1222, 1453
LmnI GCTCC 1 cut(s) 235
Lsp1109I GCAGC 2 cut(s) 1035, 1291
LweI GCATC 2 cut(s) 973, 1060
MabI ACCWGGT 1 cut(s) 858
MaeI CTAG 6 cut(s) 429, 599, 737, 984, 1085, 1253
MaeII ACGT 1 cut(s) 322
MaeIII GTNAC 4 cut(s) 314, 621, 784, 1064
MalI GATC 5 cut(s) 60, 273, 335, 1224, 1455
MbiI CCGCTC 1 cut(s) 1090
MboI GATC 5 cut(s) 58, 271, 333, 1222, 1453
MboII GAAGA 3 cut(s) 230, 1397, 1411
MflI RGATCY 3 cut(s) 333, 1222, 1453
MhlI GDGCHC 3 cut(s) 240, 265, 475
MluCI AATT 9 cut(s) 6, 72, 508, 647, 767, 869, 881, 1150, 1444
MlyI GAGTC 2 cut(s) 307, 1016
MmeI TCCRAC 1 cut(s) 195
MroI TCCGGA 1 cut(s) 1245
MroXI GAANNNNTTC 2 cut(s) 570, 767
MseI TTAA 4 cut(s) 75, 182, 629, 954
MslI CAYNNNNRTG 5 cut(s) 253, 323, 451, 497, 1125
MspA1I CMGCKG 2 cut(s) 661, 775
MspI CCGG 3 cut(s) 259, 331, 1246
MspR9I CCNGG 3 cut(s) 260, 604, 860
MvaI CCWGG 2 cut(s) 604, 860
MvnI CGCG 1 cut(s) 35
MwoI GCNNNNNNNGC 3 cut(s) 608, 1038, 1348
NciI CCSGG 1 cut(s) 260
NcoI CCATGG 1 cut(s) 743
NdeI CATATG 2 cut(s) 721, 759
NdeII GATC 5 cut(s) 58, 271, 333, 1222, 1453
NlaIII CATG 6 cut(s) 352, 516, 747, 904, 1261, 1479
NlaIV GGNNCC 4 cut(s) 28, 257, 335, 1224
NmeAIII GCCGAG 2 cut(s) 244, 637
NmuCI GTSAC 2 cut(s) 621, 1064
NspI RCATGY 1 cut(s) 352
OliI CACNNNNGTG 2 cut(s) 253, 323
PagI TCATGA 1 cut(s) 1475
PceI AGGCCT 1 cut(s) 942
PciI ACATGT 1 cut(s) 348
PdmI GAANNNNTTC 2 cut(s) 570, 767
PfeI GAWTC 3 cut(s) 11, 193, 281
PkrI GCNGC 3 cut(s) 34, 1050, 1281
PleI GAGTC 2 cut(s) 306, 1016
PmaCI CACGTG 1 cut(s) 323
PmlI CACGTG 1 cut(s) 323
PpsI GAGTC 2 cut(s) 306, 1016
Ppu21I YACGTR 1 cut(s) 323
PscI ACATGT 1 cut(s) 348
PsiI TTATAA 1 cut(s) 798
Psp6I CCWGG 2 cut(s) 602, 858
PspCI CACGTG 1 cut(s) 323
PspGI CCWGG 2 cut(s) 602, 858
PspN4I GGNNCC 4 cut(s) 28, 257, 335, 1224
PspPI GGNCC 1 cut(s) 590
PstI CTGCAG 1 cut(s) 1074
PsuI RGATCY 3 cut(s) 333, 1222, 1453
PvuII CAGCTG 1 cut(s) 661
RsaI GTAC 6 cut(s) 690, 734, 805, 994, 1119, 1186
RsaNI GTAC 6 cut(s) 689, 733, 804, 993, 1118, 1185
RseI CAYNNNNRTG 5 cut(s) 253, 323, 451, 497, 1125
SaqAI TTAA 4 cut(s) 75, 182, 629, 954
SatI GCNGC 3 cut(s) 33, 1049, 1280
Sau3AI GATC 5 cut(s) 58, 271, 333, 1222, 1453
Sau96I GGNCC 1 cut(s) 590
ScaI AGTACT 1 cut(s) 805
SchI GAGTC 2 cut(s) 307, 1016
ScrFI CCNGG 3 cut(s) 260, 604, 860
SduI GDGCHC 3 cut(s) 240, 265, 475
SexAI ACCWGGT 1 cut(s) 858
SfaNI GCATC 2 cut(s) 973, 1060
SfcI CTRYAG 4 cut(s) 807, 1070, 1107, 1366
SmiMI CAYNNNNRTG 5 cut(s) 253, 323, 451, 497, 1125
SmlI CTYRAG 1 cut(s) 908
SmoI CTYRAG 1 cut(s) 908
Sse9I AATT 9 cut(s) 6, 72, 508, 647, 767, 869, 881, 1150, 1444
SseBI AGGCCT 1 cut(s) 942
SsiI CCGC 9 cut(s) 30, 33, 355, 389, 775, 962, 1090, 1226, 1340
SspI AATATT 2 cut(s) 403, 925
SspMI CTAG 6 cut(s) 429, 599, 737, 984, 1085, 1253
StuI AGGCCT 1 cut(s) 942
StyD4I CCNGG 3 cut(s) 258, 602, 858
StyI CCWWGG 3 cut(s) 743, 1215, 1424
TaaI ACNGT 3 cut(s) 790, 1189, 1196
TaiI ACGT 1 cut(s) 325
TaqI TCGA 2 cut(s) 651, 999
TasI AATT 9 cut(s) 6, 72, 508, 647, 767, 869, 881, 1150, 1444
TatI WGTACW 2 cut(s) 803, 1117
TauI GCSGC 1 cut(s) 35
TfiI GAWTC 3 cut(s) 11, 193, 281
Tru1I TTAA 4 cut(s) 75, 182, 629, 954
Tru9I TTAA 4 cut(s) 75, 182, 629, 954
TscAI CASTG 1 cut(s) 384
TseFI GTSAC 2 cut(s) 621, 1064
TseI GCWGC 2 cut(s) 1048, 1279
Tsp45I GTSAC 2 cut(s) 621, 1064
TspDTI ATGAA 5 cut(s) 481, 493, 501, 776, 1182
TspGWI ACGGA 2 cut(s) 162, 248
TspRI CASTG 1 cut(s) 384
XapI RAATTY 5 cut(s) 508, 647, 767, 881, 1150
XbaI TCTAGA 1 cut(s) 1084
XceI RCATGY 1 cut(s) 352
XcmI CCANNNNNNNNNTGG 1 cut(s) 1034
XmiI GTMKAC 2 cut(s) 1191, 1365
XmnI GAANNNNTTC 2 cut(s) 570, 767
XspI CTAG 6 cut(s) 429, 599, 737, 984, 1085, 1253
ZrmI AGTACT 1 cut(s) 805
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.