Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0004436

Physical Location & Seq

Genomic Coordinates Forward (+)

31981691 .. 31989694

Gene Structure 8004 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1230500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1221 bp

ATGAGTATGTTCAAGCTCAATTCCCTCAGCTCGCTGCGAGCTCTGAGGCACCTCCACGCTTCCCAATCTGCCGGCGTCAGAGCGCTCACCTCCTGTTCGTCATTAATCCTCAAACAGTCGAACCCACTTCTCTCCCCCTCTCCGTCGCCGTTTCCCTTCGCACCGTCGCACAATCTGCTCGCCATTCGGAACTACCGGAGCAGCCGCGATCTCAGAAACTATGGGATTACGCCTCCCAAGAATTGGGGCGTTCGGATTGTGCCTGAGAAGAAGGCCTTTGTGATTGAGCGGTTCGGAAAGTATGTCAAGACTCTGCCTTCTGGAATCCATTTCTTAATTCCTTTTGTGGATAGAATCGCTTATGTGCATTCACTGAAAGAAGAGGTCATCAATGTTCCTGACCAGTCTGCCATTACCAAAGACAATGTCGCCCTTATGATTGATGGTGTGCTTTACGTTAGGATTGTGGATCCTATGTTGGCCTCCTATGGAGTTGACGATCCTATTAATGCAGCTATTCAGCTTGCACAGACTACCATGCGTATTGCTATCGGGAAAATCACCCTTGACAAGACATTTGAGGAAAGGGAATCCCTCAATGATGAGATAGTGACAGTTCTCAACAAGGCTGCATCCGCATGGGGGTTGGAGTGCCTCCGCTATGAAATAAAGGACATAACTCCTCCTCGTGCGGTGAGGGAATCTATGGAGATGCAAGCAGAAGCCGAACGCAAAAAGAGAGCTCAAATTCTTGAGTCTGAGGGAGAAAGGCAGGCCAACATAAATATTGCAGATGGAAAGAAGAGTGCTATAATTTTAGCAGCAGAAGGAGAAGCGCAATCCATTCTTGCTAAAGCGAATGCAACATCTCGAGGAATTTCCCTTGTGTCAAAGACTCTCAAGGAAAGTGGGGGTGCAGAGGCAGCTGCCTTGAAAATTGCTGAGCAGTATGTTGATGCCTTTGGTAATATCGCGAAACAGGGCACAACAGTGTTGCTTCCTAGTGCTTCAAATAATCCTGCTGGCATGATATCTCAAGCCTTAAGTATCTACAAAAGCTTGGTTAACAATCCTTCCACCCTTGGATCAACTGGAGGCACGCACAGTGATGCTTCCTCAGAAGGATCTGATCACGGTGTTCGAATGATCGAGGAGACAAAGAATCAAGATAATGCAAAGGAATCAGGGTTTTCGCTTCAGAACCGCAAGAAAGGAGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0006996 GO:0007005 GO:0007006 GO:0008150 GO:0009987 GO:0016043 GO:0061024 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Band_7 Band_7_C

Protein Analysis

407

Amino Acids

44.03

Weight (kDa)

9.18

Isoelectric Point (pI)

45.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Band_7	PF01145	86 - 256	8.4e-36	SPFH domain / Band 7 family
Band_7_C	PF16200	295 - 354	1.7e-23	C-terminal region of band_7

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004436)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27585 AT5G54100
fragaria_vesca	FvH4_2g05761 FvH4_5g02260 FvH4_7g33130 FvH4_7g33180
malus_domestica	MD01G1230500.v1.1 MD07G1302100.v1.1
prunus_persica	Prupe.2G321200_v2.0.a1
pyrus_communis	pycom01g23850 pycom07g27530
rosa_chinensis	RchiOBHm_Chr1g0383201 RchiOBHm_Chr6g0256651
rosa_laevigata	RLG00000014745 RLG00000026123
rosa_multiflora	Rmu_sc0007173.1_g000029 Rmu_sc0008563.1_g000002 Rmu_sc0021749.1_g000001
rosa_roxburghii	Rroxscaffold_4G00277510
rosa_rugosa	Rorug01G0439500 Rorug05G0565400 Rorug05G0565500
rosa_samantha	Rh1AG465100 Rh1BG422500 Rh1CG435700 Rh1DG455200 Rh6AG083200 Rh6BG077800 Rh6CG072400 Rh6DG069600
rosa_wichuraiana	Rw1G040740 Rw6G007510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	48
AccB7I	CCANNNNNTGG	1 cut(s)	243
AccBSI	CCGCTC	1 cut(s)	289
AccII	CGCG	2 cut(s)	207, 974
AciI	CCGC	6 cut(s)	205, 289, 636, 658, 692, 1204
AclWI	GGATC	5 cut(s)	464, 477, 494, 1093, 1132
AcsI	RAATTY	2 cut(s)	747, 876
AcuI	CTGAAG	1 cut(s)	1181
AcyI	GRCGYC	1 cut(s)	75
AfeI	AGCGCT	1 cut(s)	84
AfiI	CCNNNNNNNGG	2 cut(s)	243, 642
AflII	CTTAAG	1 cut(s)	1042
AgsI	TTSAA	3 cut(s)	13, 934, 1011
AleI	CACNNNNGTG	1 cut(s)	989
AluBI	AGCT	8 cut(s)	16, 30, 41, 515, 523, 743, 926, 1059
AluI	AGCT	8 cut(s)	16, 30, 41, 515, 523, 743, 926, 1059
Alw21I	GWGCWC	2 cut(s)	43, 745
Alw26I	GTCTC	1 cut(s)	1148
AlwI	GGATC	5 cut(s)	464, 477, 494, 1093, 1132
Ama87I	CYCGRG	1 cut(s)	870
Aor51HI	AGCGCT	1 cut(s)	84
AoxI	GGCC	3 cut(s)	273, 480, 774
ApeKI	GCWGC	7 cut(s)	34, 201, 512, 629, 821, 923, 926
ApoI	RAATTY	2 cut(s)	747, 876
ArsI	GACNNNNNNTTYG	4 cut(s)	411, 443, 560, 592
AseI	ATTAAT	2 cut(s)	104, 507
AspLEI	GCGC	2 cut(s)	85, 838
AsuHPI	GGTGA	3 cut(s)	79, 553, 706
AsuII	TTCGAA	1 cut(s)	1141
AvaI	CYCGRG	1 cut(s)	870
BaeGI	GKGCMC	1 cut(s)	986
BamHI	GGATCC	1 cut(s)	469
BanI	GGYRCC	1 cut(s)	48
BanII	GRGCYC	2 cut(s)	43, 745
BauI	CACGAG	1 cut(s)	687
Bbv12I	GWGCWC	2 cut(s)	43, 745
BbvCI	CCTCAGC	1 cut(s)	26
BbvI	GCAGC	7 cut(s)	21, 213, 524, 616, 833, 913, 935
BccI	CCATC	2 cut(s)	437, 788
BceAI	ACGGC	1 cut(s)	133
BclI	TGATCA	1 cut(s)	1129
BcoDI	GTCTC	1 cut(s)	1148
BfaI	CTAG	1 cut(s)	1002
BfoI	RGCGCY	1 cut(s)	86
BfrI	CTTAAG	1 cut(s)	1042
BisI	GCNGC	8 cut(s)	35, 202, 205, 513, 630, 822, 924, 927
BlpI	GCTNAGC	1 cut(s)	942
BlsI	GCNGC	8 cut(s)	36, 203, 206, 514, 631, 823, 925, 928
BmeT110I	CYCGRG	1 cut(s)	870
BmiI	GGNNCC	2 cut(s)	50, 471
BmsI	GCATC	4 cut(s)	641, 702, 946, 1099
BpmI	CTGGAG	1 cut(s)	1113
Bpu10I	CCTNAGC	1 cut(s)	26
Bpu1102I	GCTNAGC	1 cut(s)	942
Bpu14I	TTCGAA	1 cut(s)	1141
BpuEI	CTTGAG	3 cut(s)	773, 884, 1020
BsaHI	GRCGYC	1 cut(s)	75
BsaJI	CCNNGG	1 cut(s)	1081
BsaWI	WCCGGW	1 cut(s)	195
Bsc4I	CCNNNNNNNGG	2 cut(s)	243, 642
Bse118I	RCCGGY	1 cut(s)	71
Bse1I	ACTGG	2 cut(s)	403, 1096
BseDI	CCNNGG	1 cut(s)	1081
BseGI	GGATG	1 cut(s)	632
BseLI	CCNNNNNNNGG	2 cut(s)	243, 642
BseMII	CTCAG	7 cut(s)	35, 40, 226, 255, 750, 933, 1131
BseNI	ACTGG	2 cut(s)	403, 1096
BseRI	GAGGAG	3 cut(s)	672, 675, 1166
BseSI	GKGCMC	1 cut(s)	986
BseXI	GCAGC	7 cut(s)	21, 213, 524, 616, 833, 913, 935
BsgI	GTGCAG	1 cut(s)	936
Bsh1236I	CGCG	2 cut(s)	207, 974
BshFI	GGCC	3 cut(s)	275, 482, 776
BshNI	GGYRCC	1 cut(s)	48
BsiHKAI	GWGCWC	2 cut(s)	43, 745
BsiHKCI	CYCGRG	1 cut(s)	870
BsiSI	CCGG	2 cut(s)	72, 196
BslI	CCNNNNNNNGG	2 cut(s)	243, 642
BsmAI	GTCTC	1 cut(s)	1148
BsmI	GAATGC	2 cut(s)	367, 865
BsnI	GGCC	3 cut(s)	275, 482, 776
BsoBI	CYCGRG	1 cut(s)	870
Bsp119I	TTCGAA	1 cut(s)	1141
Bsp1286I	GDGCHC	3 cut(s)	43, 745, 986
Bsp143I	GATC	7 cut(s)	208, 469, 499, 1085, 1124, 1129, 1146
Bsp1720I	GCTNAGC	1 cut(s)	942
Bsp68I	TCGCGA	1 cut(s)	974
BspACI	CCGC	6 cut(s)	205, 289, 636, 658, 692, 1204
BspANI	GGCC	3 cut(s)	275, 482, 776
BspCNI	CTCAG	7 cut(s)	36, 39, 225, 256, 751, 934, 1130
BspFNI	CGCG	2 cut(s)	207, 974
BspLI	GGNNCC	2 cut(s)	50, 471
BspPI	GGATC	5 cut(s)	464, 477, 494, 1093, 1132
BspT104I	TTCGAA	1 cut(s)	1141
BspT107I	GGYRCC	1 cut(s)	48
BspTI	CTTAAG	1 cut(s)	1042
BsrBI	CCGCTC	1 cut(s)	289
BsrFI	RCCGGY	1 cut(s)	71
BsrI	ACTGG	2 cut(s)	403, 1096
BssAI	RCCGGY	1 cut(s)	71
BssECI	CCNNGG	1 cut(s)	1081
BssMI	GATC	7 cut(s)	208, 469, 499, 1085, 1124, 1129, 1146
BssNI	GRCGYC	1 cut(s)	75
BssSI	CACGAG	1 cut(s)	687
BssT1I	CCWWGG	1 cut(s)	1081
Bst2BI	CACGAG	1 cut(s)	687
Bst4CI	ACNGT	6 cut(s)	117, 165, 616, 991, 1106, 1136
Bst6I	CTCTTC	2 cut(s)	375, 797
BstACI	GRCGYC	1 cut(s)	75
BstAFI	CTTAAG	1 cut(s)	1042
BstAPI	GCANNNNNTGC	1 cut(s)	175
BstBI	TTCGAA	1 cut(s)	1141
BstC8I	GCNNGC	9 cut(s)	32, 39, 73, 180, 525, 717, 774, 1024, 1100
BstDEI	CTNAG	7 cut(s)	26, 44, 212, 264, 759, 942, 1117
BstF5I	GGATG	1 cut(s)	632
BstFNI	CGCG	2 cut(s)	207, 974
BstH2I	RGCGCY	1 cut(s)	86
BstHHI	GCGC	2 cut(s)	85, 838
BstKTI	GATC	7 cut(s)	211, 472, 502, 1088, 1127, 1132, 1149
BstMAI	GTCTC	1 cut(s)	1148
BstMBI	GATC	7 cut(s)	208, 469, 499, 1085, 1124, 1129, 1146
BstMWI	GCNNNNNNNGC	3 cut(s)	175, 635, 923
BstSLI	GKGCMC	1 cut(s)	986
BstUI	CGCG	2 cut(s)	207, 974
BstV1I	GCAGC	7 cut(s)	21, 213, 524, 616, 833, 913, 935
BstX2I	RGATCY	2 cut(s)	469, 1124
BstYI	RGATCY	2 cut(s)	469, 1124
BsuRI	GGCC	3 cut(s)	275, 482, 776
BtsCI	GGATG	1 cut(s)	632
BtsIMutI	CAGTG	3 cut(s)	371, 996, 1111
BtuMI	TCGCGA	1 cut(s)	974
Cac8I	GCNNGC	9 cut(s)	32, 39, 73, 180, 525, 717, 774, 1024, 1100
CfoI	GCGC	2 cut(s)	85, 838
Cfr10I	RCCGGY	1 cut(s)	71
CseI	GACGC	1 cut(s)	64
CspCI	CAANNNNNGTGG	2 cut(s)	889, 924
CviAII	CATG	3 cut(s)	538, 639, 1027
DdeI	CTNAG	7 cut(s)	26, 44, 212, 264, 759, 942, 1117
DpnI	GATC	7 cut(s)	210, 471, 501, 1087, 1126, 1131, 1148
DpnII	GATC	7 cut(s)	208, 469, 499, 1085, 1124, 1129, 1146
Eam1104I	CTCTTC	2 cut(s)	375, 797
EarI	CTCTTC	2 cut(s)	375, 797
Ecl136II	GAGCTC	2 cut(s)	41, 743
Eco130I	CCWWGG	1 cut(s)	1081
Eco147I	AGGCCT	1 cut(s)	275
Eco24I	GRGCYC	2 cut(s)	43, 745
Eco32I	GATATC	1 cut(s)	1032
Eco47III	AGCGCT	1 cut(s)	84
Eco53kI	GAGCTC	2 cut(s)	41, 743
Eco57I	CTGAAG	1 cut(s)	1181
Eco88I	CYCGRG	1 cut(s)	870
EcoICRI	GAGCTC	2 cut(s)	41, 743
EcoRV	GATATC	1 cut(s)	1032
EcoT14I	CCWWGG	1 cut(s)	1081
EcoT38I	GRGCYC	2 cut(s)	43, 745
ErhI	CCWWGG	1 cut(s)	1081
FaeI	CATG	3 cut(s)	541, 642, 1030
FalI	AAGNNNNNCTT	2 cut(s)	260, 292
FatI	CATG	3 cut(s)	537, 638, 1026
FbaI	TGATCA	1 cut(s)	1129
Fnu4HI	GCNGC	8 cut(s)	35, 202, 205, 513, 630, 822, 924, 927
FokI	GGATG	1 cut(s)	619
FriOI	GRGCYC	2 cut(s)	43, 745
Fsp4HI	GCNGC	8 cut(s)	35, 202, 205, 513, 630, 822, 924, 927
FspBI	CTAG	1 cut(s)	1002
GlaI	GCGC	2 cut(s)	84, 837
GluI	GCNGC	8 cut(s)	35, 202, 205, 513, 630, 822, 924, 927
GsuI	CTGGAG	1 cut(s)	1113
HaeII	RGCGCY	1 cut(s)	86
HaeIII	GGCC	3 cut(s)	275, 482, 776
HapII	CCGG	2 cut(s)	72, 196
HgaI	GACGC	1 cut(s)	64
HhaI	GCGC	2 cut(s)	85, 838
Hin1I	GRCGYC	1 cut(s)	75
Hin1II	CATG	3 cut(s)	541, 642, 1030
Hin6I	GCGC	2 cut(s)	83, 836
HinP1I	GCGC	2 cut(s)	83, 836
HincII	GTYRAC	2 cut(s)	496, 1066
HindII	GTYRAC	2 cut(s)	496, 1066
HindIII	AAGCTT	1 cut(s)	1057
HinfI	GANTC	9 cut(s)	310, 324, 354, 590, 701, 755, 895, 1162, 1181
HpaI	GTTAAC	1 cut(s)	1066
HpaII	CCGG	2 cut(s)	72, 196
HphI	GGTGA	3 cut(s)	79, 553, 706
Hpy166II	GTNNAC	2 cut(s)	496, 1066
Hpy188III	TCNNGA	8 cut(s)	307, 321, 398, 553, 752, 870, 973, 1166
Hpy8I	GTNNAC	2 cut(s)	496, 1066
Hpy99I	CGWCG	2 cut(s)	148, 169
HpyAV	CCTTC	6 cut(s)	166, 265, 327, 821, 1083, 1115
HpyCH4III	ACNGT	6 cut(s)	117, 165, 616, 991, 1106, 1136
HpyCH4IV	ACGT	1 cut(s)	456
HpyCH4V	TGCA	9 cut(s)	367, 512, 527, 632, 715, 791, 863, 917, 1175
HpyF10VI	GCNNNNNNNGC	3 cut(s)	175, 635, 923
HpyF3I	CTNAG	7 cut(s)	26, 44, 212, 264, 759, 942, 1117
HpySE526I	ACGT	1 cut(s)	456
Hsp92I	GRCGYC	1 cut(s)	75
Hsp92II	CATG	3 cut(s)	541, 642, 1030
HspAI	GCGC	2 cut(s)	83, 836
KroI	GCCGGC	1 cut(s)	71
KroNI	GCCGGC	1 cut(s)	73
Ksp22I	TGATCA	1 cut(s)	1129
KspAI	GTTAAC	1 cut(s)	1066
Kzo9I	GATC	7 cut(s)	208, 469, 499, 1085, 1124, 1129, 1146
LmnI	GCTCC	2 cut(s)	198, 1214
Lsp1109I	GCAGC	7 cut(s)	21, 213, 524, 616, 833, 913, 935
LweI	GCATC	4 cut(s)	641, 702, 946, 1099
MaeI	CTAG	1 cut(s)	1002
MaeII	ACGT	1 cut(s)	456
MaeIII	GTNAC	1 cut(s)	610
MalI	GATC	7 cut(s)	210, 471, 501, 1087, 1126, 1131, 1148
MbiI	CCGCTC	1 cut(s)	289
MboI	GATC	7 cut(s)	208, 469, 499, 1085, 1124, 1129, 1146
MboII	GAAGA	3 cut(s)	280, 392, 814
MflI	RGATCY	2 cut(s)	469, 1124
MhlI	GDGCHC	3 cut(s)	43, 745, 986
MluCI	AATT	7 cut(s)	19, 241, 336, 747, 813, 876, 936
MlyI	GAGTC	3 cut(s)	304, 764, 889
MmeI	TCCRAC	1 cut(s)	627
MroNI	GCCGGC	1 cut(s)	71
MseI	TTAA	5 cut(s)	104, 335, 507, 1043, 1065
MslI	CAYNNNNRTG	3 cut(s)	637, 989, 1107
MspA1I	CMGCKG	1 cut(s)	926
MspCI	CTTAAG	1 cut(s)	1042
MspI	CCGG	2 cut(s)	72, 196
Mva1269I	GAATGC	2 cut(s)	367, 865
MvnI	CGCG	2 cut(s)	207, 974
MwoI	GCNNNNNNNGC	3 cut(s)	175, 635, 923
NaeI	GCCGGC	1 cut(s)	73
NdeII	GATC	7 cut(s)	208, 469, 499, 1085, 1124, 1129, 1146
NgoMIV	GCCGGC	1 cut(s)	71
NlaIII	CATG	3 cut(s)	541, 642, 1030
NlaIV	GGNNCC	2 cut(s)	50, 471
NmuCI	GTSAC	1 cut(s)	610
NruI	TCGCGA	1 cut(s)	974
NspV	TTCGAA	1 cut(s)	1141
OliI	CACNNNNGTG	1 cut(s)	989
PaeR7I	CTCGAG	1 cut(s)	870
PceI	AGGCCT	1 cut(s)	275
PctI	GAATGC	2 cut(s)	367, 865
PdiI	GCCGGC	1 cut(s)	73
PfeI	GAWTC	6 cut(s)	324, 354, 590, 701, 1162, 1181
PflFI	GACNNNGTC	1 cut(s)	425
PflMI	CCANNNNNTGG	1 cut(s)	243
PkrI	GCNGC	8 cut(s)	36, 203, 206, 514, 631, 823, 925, 928
PleI	GAGTC	3 cut(s)	304, 763, 889
PpsI	GAGTC	3 cut(s)	304, 763, 889
PshBI	ATTAAT	2 cut(s)	104, 507
Psp124BI	GAGCTC	2 cut(s)	43, 745
PspN4I	GGNNCC	2 cut(s)	50, 471
PsuI	RGATCY	2 cut(s)	469, 1124
PsyI	GACNNNGTC	1 cut(s)	425
PvuII	CAGCTG	1 cut(s)	926
RruI	TCGCGA	1 cut(s)	974
RseI	CAYNNNNRTG	3 cut(s)	637, 989, 1107
SacI	GAGCTC	2 cut(s)	43, 745
SaqAI	TTAA	5 cut(s)	104, 335, 507, 1043, 1065
SatI	GCNGC	8 cut(s)	35, 202, 205, 513, 630, 822, 924, 927
Sau3AI	GATC	7 cut(s)	208, 469, 499, 1085, 1124, 1129, 1146
SchI	GAGTC	3 cut(s)	304, 764, 889
SduI	GDGCHC	3 cut(s)	43, 745, 986
SfaNI	GCATC	4 cut(s)	641, 702, 946, 1099
Sfr274I	CTCGAG	1 cut(s)	870
SfuI	TTCGAA	1 cut(s)	1141
SlaI	CTCGAG	1 cut(s)	870
SmiMI	CAYNNNNRTG	3 cut(s)	637, 989, 1107
SmlI	CTYRAG	5 cut(s)	752, 870, 899, 1035, 1042
SmoI	CTYRAG	5 cut(s)	752, 870, 899, 1035, 1042
Sse9I	AATT	7 cut(s)	19, 241, 336, 747, 813, 876, 936
SseBI	AGGCCT	1 cut(s)	275
SsiI	CCGC	6 cut(s)	205, 289, 636, 658, 692, 1204
SspI	AATATT	1 cut(s)	787
SspMI	CTAG	1 cut(s)	1002
SstI	GAGCTC	2 cut(s)	43, 745
StuI	AGGCCT	1 cut(s)	275
StyI	CCWWGG	1 cut(s)	1081
TaaI	ACNGT	6 cut(s)	117, 165, 616, 991, 1106, 1136
TaiI	ACGT	1 cut(s)	459
TaqI	TCGA	4 cut(s)	119, 871, 1141, 1149
TasI	AATT	7 cut(s)	19, 241, 336, 747, 813, 876, 936
TauI	GCSGC	1 cut(s)	207
TfiI	GAWTC	6 cut(s)	324, 354, 590, 701, 1162, 1181
Tru1I	TTAA	5 cut(s)	104, 335, 507, 1043, 1065
Tru9I	TTAA	5 cut(s)	104, 335, 507, 1043, 1065
TscAI	CASTG	3 cut(s)	378, 996, 1111
TseFI	GTSAC	1 cut(s)	610
TseI	GCWGC	7 cut(s)	34, 201, 512, 629, 821, 923, 926
Tsp45I	GTSAC	1 cut(s)	610
TspDTI	ATGAA	1 cut(s)	678
TspGWI	ACGGA	1 cut(s)	132
TspRI	CASTG	3 cut(s)	378, 996, 1111
Tth111I	GACNNNGTC	1 cut(s)	425
Van91I	CCANNNNNTGG	1 cut(s)	243
Vha464I	CTTAAG	1 cut(s)	1042
VspI	ATTAAT	2 cut(s)	104, 507
XapI	RAATTY	2 cut(s)	747, 876
XhoI	CTCGAG	1 cut(s)	870
XspI	CTAG	1 cut(s)	1002

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.