pycom07g27530

Stomatin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0004436
Physical Location & Seq
Forward (+)
27224993 .. 27228006
3014 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g27530.2

Sequence Viewer

Length: 1230 bp
ATGAGTATGTTCAAGCTGAATTCCGTCAATTCGCTGCGAGCTCTGAGGCGCCTCCACACCTCCCAATCCGCCGGCGTCAGAGCGCTCACCACCTGTTCGTCATTAATCCTCAAACAGTCGACCCCACTTCTCTCTCCCTCTCCGTCTCCGTCTGCGTTCGCACCGTCGCACAATCTGCTTGCCGTTCGAAACTACCGGAGCAGCCGCGATCTCAGAAGCTATGGGATTACGCCTCCCAGGAATTGGGGCATTCGGATTGTGCCTGAGAAGAAGGCCTTTGTGATTGAGCGGTTCGGAAAGTACATCAGGACTCTGCCGTCTGGAATCCATTTCTTGATTCCGTTTGTGGATAGAATCGCTTATGTGCATTCCTTGAAAGAAGAGGTCATCAATGTTCTTGACCAGTCCGCCATTACCAAAGACAATGTCGCCCTTATGATTGATGGTGTGCTTTATGTTAGGATTGTGGATCGATCTATGCTGGCCTCCTATGGTGTTGACAATCCTATTAATGCAGCTATTCAGCTTGCACAGACTACCATGCGTATTGCGATCGGGAAAATCACTCTTGACAAGACGTTTGAGGAAAGAGAATCTCTCAATGATGAGATAGTGACAATTCTCAACAAGGCTGCATCAGCATGGGGGTTGGAGTGCCTCCGCTATGAAATAAAGGACATAACTCCTCCTCGTGCGGTGAGGGAAGCTATGGAGATGCAAGCAGAAGCAGAACGAAAAAAGAGAGCTCAAATTCTTGAGTCTGAGGGAGAAAGGCAGGCTAACATAAACATCGCAGATGGAAAGAAGAGTGCTATAATTTTAGCGGCAGAAGGAGAAGCGCAATCCATTCTTGCTAAAGCAAATGCAACATCTCGAGGAATTTCCCTTGTGTCAAAGTCTCTCAATGAAAGTGGGGGTGCAGAGGCAGCTGCTTTGAAAATTGCTGAGCAGTACGTTGGTGCCTTTGGTAATATCGCTAAACAGGGCACAACAGTGTTGCTTCCTAGTGCTTCAAATAATCCTGCTGGCATGATCTCTCAAGCCTTAAGTATCTACAAAAGCTTGGTTAGCAATCCTTCCACCCTTGGATCAACTGGAGGCACGCACAGTGATGATGCTTCCTCTGCAGGATCTGATGATCATGGTGTTCGAATGATTGAGGAAACAACTAATCAAGATAATGCAAAGGAATCAGGATTTTCGCTTCAGAACCGCAAGAAAGGAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006996 GO:0007005 GO:0007006 GO:0008150 GO:0009987 GO:0016043 GO:0061024 GO:0071840
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Band_7 Band_7_C

Protein Analysis

410

Amino Acids

44.24

Weight (kDa)

9.33

Isoelectric Point (pI)

45.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 316
AccB1I GGYRCC 2 cut(s) 48, 959
AccB7I CCANNNNNTGG 1 cut(s) 243
AccBSI CCGCTC 1 cut(s) 289
AccI GTMKAC 1 cut(s) 119
AccII CGCG 1 cut(s) 207
AciI CCGC 8 cut(s) 69, 205, 289, 408, 661, 695, 824, 1213
AclWI GGATC 3 cut(s) 477, 1096, 1138
AcsI RAATTY 3 cut(s) 19, 750, 879
AcuI CTGAAG 1 cut(s) 1190
AcyI GRCGYC 2 cut(s) 49, 75
AfaI GTAC 2 cut(s) 302, 953
AfeI AGCGCT 1 cut(s) 84
AfiI CCNNNNNNNGG 1 cut(s) 243
AflII CTTAAG 1 cut(s) 1045
AgsI TTSAA 4 cut(s) 13, 376, 937, 1014
AjnI CCWGG 1 cut(s) 236
AleI CACNNNNGTG 1 cut(s) 992
AluBI AGCT 9 cut(s) 16, 41, 219, 518, 526, 707, 746, 929, 1062
AluI AGCT 9 cut(s) 16, 41, 219, 518, 526, 707, 746, 929, 1062
Alw21I GWGCWC 2 cut(s) 43, 748
Alw26I GTCTC 2 cut(s) 150, 903
AlwI GGATC 3 cut(s) 477, 1096, 1138
AlwNI CAGNNNCTG 1 cut(s) 1133
Ama87I CYCGRG 1 cut(s) 873
Aor51HI AGCGCT 1 cut(s) 84
AoxI GGCC 2 cut(s) 273, 483
ApeKI GCWGC 6 cut(s) 34, 201, 515, 632, 926, 929
ApoI RAATTY 3 cut(s) 19, 750, 879
ArsI GACNNNNNNTTYG 4 cut(s) 411, 443, 563, 595
AseI ATTAAT 2 cut(s) 104, 510
AspLEI GCGC 3 cut(s) 51, 85, 841
AsuHPI GGTGA 2 cut(s) 79, 709
AsuII TTCGAA 2 cut(s) 187, 1150
AvaI CYCGRG 1 cut(s) 873
BaeGI GKGCMC 1 cut(s) 989
BanI GGYRCC 2 cut(s) 48, 959
BanII GRGCYC 2 cut(s) 43, 748
BauI CACGAG 1 cut(s) 690
Bbv12I GWGCWC 2 cut(s) 43, 748
BbvI GCAGC 6 cut(s) 21, 213, 527, 619, 916, 938
BccI CCATC 2 cut(s) 437, 791
BceAI ACGGC 2 cut(s) 167, 301
BciT130I CCWGG 1 cut(s) 238
BclI TGATCA 1 cut(s) 1138
BcoDI GTCTC 2 cut(s) 150, 903
BfaI CTAG 1 cut(s) 1005
BfmI CTRYAG 1 cut(s) 1125
BfoI RGCGCY 2 cut(s) 52, 86
BfrI CTTAAG 1 cut(s) 1045
BisI GCNGC 8 cut(s) 35, 202, 205, 516, 633, 825, 927, 930
BlpI GCTNAGC 1 cut(s) 945
BlsI GCNGC 8 cut(s) 36, 203, 206, 517, 634, 826, 928, 931
Bme1390I CCNGG 1 cut(s) 238
BmeT110I CYCGRG 1 cut(s) 873
BmiI GGNNCC 2 cut(s) 50, 961
BmrFI CCNGG 1 cut(s) 238
BmsI GCATC 3 cut(s) 644, 705, 1105
BplI GAGNNNNNCTC 2 cut(s) 582, 614
BpmI CTGGAG 1 cut(s) 1116
Bpu1102I GCTNAGC 1 cut(s) 945
Bpu14I TTCGAA 2 cut(s) 187, 1150
BpuEI CTTGAG 2 cut(s) 776, 1023
Bsa29I ATCGAT 1 cut(s) 472
BsaHI GRCGYC 2 cut(s) 49, 75
BsaJI CCNNGG 2 cut(s) 236, 1084
BsaWI WCCGGW 1 cut(s) 195
Bsc4I CCNNNNNNNGG 1 cut(s) 243
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 2 cut(s) 403, 1099
BseBI CCWGG 1 cut(s) 238
BseCI ATCGAT 1 cut(s) 472
BseDI CCNNGG 2 cut(s) 236, 1084
BseLI CCNNNNNNNGG 1 cut(s) 243
BseMII CTCAG 5 cut(s) 35, 226, 255, 753, 936
BseNI ACTGG 2 cut(s) 403, 1099
BseRI GAGGAG 2 cut(s) 675, 678
BseSI GKGCMC 1 cut(s) 989
BseXI GCAGC 6 cut(s) 21, 213, 527, 619, 916, 938
BsgI GTGCAG 1 cut(s) 939
Bsh1236I CGCG 1 cut(s) 207
Bsh1285I CGRYCG 1 cut(s) 555
BshFI GGCC 2 cut(s) 275, 485
BshNI GGYRCC 2 cut(s) 48, 959
BshVI ATCGAT 1 cut(s) 472
BsiEI CGRYCG 1 cut(s) 555
BsiHKAI GWGCWC 2 cut(s) 43, 748
BsiHKCI CYCGRG 1 cut(s) 873
BsiSI CCGG 2 cut(s) 72, 196
BslI CCNNNNNNNGG 1 cut(s) 243
BsmAI GTCTC 2 cut(s) 150, 903
BsmBI CGTCTC 1 cut(s) 150
BsmI GAATGC 2 cut(s) 249, 367
BsnI GGCC 2 cut(s) 275, 485
BsoBI CYCGRG 1 cut(s) 873
Bsp119I TTCGAA 2 cut(s) 187, 1150
Bsp1286I GDGCHC 3 cut(s) 43, 748, 989
Bsp143I GATC 8 cut(s) 208, 469, 473, 552, 1032, 1088, 1130, 1138
Bsp1720I GCTNAGC 1 cut(s) 945
BspACI CCGC 8 cut(s) 69, 205, 289, 408, 661, 695, 824, 1213
BspANI GGCC 2 cut(s) 275, 485
BspCNI CTCAG 5 cut(s) 36, 225, 256, 754, 937
BspDI ATCGAT 1 cut(s) 472
BspFNI CGCG 1 cut(s) 207
BspLI GGNNCC 2 cut(s) 50, 961
BspMAI CTGCAG 1 cut(s) 1129
BspPI GGATC 3 cut(s) 477, 1096, 1138
BspT104I TTCGAA 2 cut(s) 187, 1150
BspT107I GGYRCC 2 cut(s) 48, 959
BspTI CTTAAG 1 cut(s) 1045
BsrBI CCGCTC 1 cut(s) 289
BsrFI RCCGGY 1 cut(s) 71
BsrI ACTGG 2 cut(s) 403, 1099
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 2 cut(s) 236, 1084
BssMI GATC 8 cut(s) 208, 469, 473, 552, 1032, 1088, 1130, 1138
BssNI GRCGYC 2 cut(s) 49, 75
BssSI CACGAG 1 cut(s) 690
BssT1I CCWWGG 1 cut(s) 1084
Bst2BI CACGAG 1 cut(s) 690
Bst2UI CCWGG 1 cut(s) 238
Bst4CI ACNGT 4 cut(s) 117, 165, 994, 1109
Bst6I CTCTTC 2 cut(s) 375, 800
BstACI GRCGYC 2 cut(s) 49, 75
BstAFI CTTAAG 1 cut(s) 1045
BstAPI GCANNNNNTGC 1 cut(s) 175
BstBI TTCGAA 2 cut(s) 187, 1150
BstC8I GCNNGC 9 cut(s) 39, 73, 180, 483, 528, 720, 777, 1027, 1103
BstDEI CTNAG 5 cut(s) 44, 212, 264, 762, 945
BstFNI CGCG 1 cut(s) 207
BstH2I RGCGCY 2 cut(s) 52, 86
BstHHI GCGC 3 cut(s) 51, 85, 841
BstKTI GATC 8 cut(s) 211, 472, 476, 555, 1035, 1091, 1133, 1141
BstMAI GTCTC 2 cut(s) 150, 903
BstMBI GATC 8 cut(s) 208, 469, 473, 552, 1032, 1088, 1130, 1138
BstMCI CGRYCG 1 cut(s) 555
BstMWI GCNNNNNNNGC 5 cut(s) 175, 638, 926, 1068, 1124
BstNI CCWGG 1 cut(s) 238
BstSCI CCNGG 1 cut(s) 236
BstSFI CTRYAG 1 cut(s) 1125
BstSLI GKGCMC 1 cut(s) 989
BstUI CGCG 1 cut(s) 207
BstV1I GCAGC 6 cut(s) 21, 213, 527, 619, 916, 938
BstX2I RGATCY 1 cut(s) 1130
BstYI RGATCY 1 cut(s) 1130
Bsu15I ATCGAT 1 cut(s) 472
BsuRI GGCC 2 cut(s) 275, 485
BsuTUI ATCGAT 1 cut(s) 472
BtgZI GCGATG 1 cut(s) 775
BtsIMutI CAGTG 2 cut(s) 999, 1114
Cac8I GCNNGC 9 cut(s) 39, 73, 180, 483, 528, 720, 777, 1027, 1103
CaiI CAGNNNCTG 1 cut(s) 1133
CfoI GCGC 3 cut(s) 51, 85, 841
Cfr10I RCCGGY 1 cut(s) 71
ClaI ATCGAT 1 cut(s) 472
CseI GACGC 1 cut(s) 64
Csp6I GTAC 2 cut(s) 301, 952
CspCI CAANNNNNGTGG 2 cut(s) 892, 927
CviAII CATG 4 cut(s) 541, 642, 1030, 1142
CviQI GTAC 2 cut(s) 301, 952
DdeI CTNAG 5 cut(s) 44, 212, 264, 762, 945
DinI GGCGCC 1 cut(s) 50
DpnI GATC 8 cut(s) 210, 471, 475, 554, 1034, 1090, 1132, 1140
DpnII GATC 8 cut(s) 208, 469, 473, 552, 1032, 1088, 1130, 1138
DrdI GACNNNNNNGTC 1 cut(s) 316
DseDI GACNNNNNNGTC 1 cut(s) 316
Eam1104I CTCTTC 2 cut(s) 375, 800
EarI CTCTTC 2 cut(s) 375, 800
EciI GGCGGA 2 cut(s) 58, 397
Ecl136II GAGCTC 2 cut(s) 41, 746
Eco130I CCWWGG 1 cut(s) 1084
Eco147I AGGCCT 1 cut(s) 275
Eco24I GRGCYC 2 cut(s) 43, 748
Eco47III AGCGCT 1 cut(s) 84
Eco53kI GAGCTC 2 cut(s) 41, 746
Eco57I CTGAAG 1 cut(s) 1190
Eco88I CYCGRG 1 cut(s) 873
EcoICRI GAGCTC 2 cut(s) 41, 746
EcoRI GAATTC 1 cut(s) 19
EcoRII CCWGG 1 cut(s) 236
EcoT14I CCWWGG 1 cut(s) 1084
EcoT38I GRGCYC 2 cut(s) 43, 748
EgeI GGCGCC 1 cut(s) 50
EheI GGCGCC 1 cut(s) 50
ErhI CCWWGG 1 cut(s) 1084
Esp3I CGTCTC 1 cut(s) 150
FaeI CATG 4 cut(s) 544, 645, 1033, 1145
FalI AAGNNNNNCTT 2 cut(s) 260, 292
FatI CATG 4 cut(s) 540, 641, 1029, 1141
FbaI TGATCA 1 cut(s) 1138
FblI GTMKAC 1 cut(s) 119
Fnu4HI GCNGC 8 cut(s) 35, 202, 205, 516, 633, 825, 927, 930
FriOI GRGCYC 2 cut(s) 43, 748
Fsp4HI GCNGC 8 cut(s) 35, 202, 205, 516, 633, 825, 927, 930
FspBI CTAG 1 cut(s) 1005
GlaI GCGC 3 cut(s) 50, 84, 840
GluI GCNGC 8 cut(s) 35, 202, 205, 516, 633, 825, 927, 930
GsuI CTGGAG 1 cut(s) 1116
HaeII RGCGCY 2 cut(s) 52, 86
HaeIII GGCC 2 cut(s) 275, 485
HapII CCGG 2 cut(s) 72, 196
HgaI GACGC 1 cut(s) 64
HhaI GCGC 3 cut(s) 51, 85, 841
Hin1I GRCGYC 2 cut(s) 49, 75
Hin1II CATG 4 cut(s) 544, 645, 1033, 1145
Hin6I GCGC 3 cut(s) 49, 83, 839
HinP1I GCGC 3 cut(s) 49, 83, 839
HincII GTYRAC 2 cut(s) 120, 499
HindII GTYRAC 2 cut(s) 120, 499
HindIII AAGCTT 1 cut(s) 1060
HinfI GANTC 7 cut(s) 310, 324, 337, 354, 593, 758, 1190
HpaII CCGG 2 cut(s) 72, 196
HphI GGTGA 2 cut(s) 79, 709
Hpy166II GTNNAC 2 cut(s) 120, 499
Hpy188I TCNGA 8 cut(s) 45, 80, 215, 255, 296, 763, 1135, 1209
Hpy8I GTNNAC 2 cut(s) 120, 499
Hpy99I CGWCG 1 cut(s) 169
HpyAV CCTTC 3 cut(s) 265, 824, 1086
HpyCH4III ACNGT 4 cut(s) 117, 165, 994, 1109
HpyCH4IV ACGT 2 cut(s) 578, 954
HpyCH4V TGCA 9 cut(s) 367, 515, 530, 635, 718, 866, 920, 1127, 1184
HpyF10VI GCNNNNNNNGC 5 cut(s) 175, 638, 926, 1068, 1124
HpyF3I CTNAG 5 cut(s) 44, 212, 264, 762, 945
HpySE526I ACGT 2 cut(s) 578, 954
Hsp92I GRCGYC 2 cut(s) 49, 75
Hsp92II CATG 4 cut(s) 544, 645, 1033, 1145
HspAI GCGC 3 cut(s) 49, 83, 839
KasI GGCGCC 1 cut(s) 48
KroI GCCGGC 1 cut(s) 71
KroNI GCCGGC 1 cut(s) 73
Ksp22I TGATCA 1 cut(s) 1138
Kzo9I GATC 8 cut(s) 208, 469, 473, 552, 1032, 1088, 1130, 1138
LmnI GCTCC 2 cut(s) 198, 1223
Lsp1109I GCAGC 6 cut(s) 21, 213, 527, 619, 916, 938
LweI GCATC 3 cut(s) 644, 705, 1105
MaeI CTAG 1 cut(s) 1005
MaeII ACGT 2 cut(s) 578, 954
MaeIII GTNAC 1 cut(s) 613
MalI GATC 8 cut(s) 210, 471, 475, 554, 1034, 1090, 1132, 1140
MbiI CCGCTC 1 cut(s) 289
MboI GATC 8 cut(s) 208, 469, 473, 552, 1032, 1088, 1130, 1138
MboII GAAGA 3 cut(s) 280, 392, 817
MflI RGATCY 1 cut(s) 1130
MhlI GDGCHC 3 cut(s) 43, 748, 989
MluCI AATT 8 cut(s) 19, 28, 241, 618, 750, 816, 879, 939
Mly113I GGCGCC 1 cut(s) 49
MlyI GAGTC 2 cut(s) 304, 767
MmeI TCCRAC 1 cut(s) 630
MreI CGCCGGCG 1 cut(s) 71
MroNI GCCGGC 1 cut(s) 71
MseI TTAA 3 cut(s) 104, 510, 1046
MslI CAYNNNNRTG 3 cut(s) 640, 992, 1110
MspA1I CMGCKG 1 cut(s) 929
MspCI CTTAAG 1 cut(s) 1045
MspI CCGG 2 cut(s) 72, 196
MspR9I CCNGG 1 cut(s) 238
Mva1269I GAATGC 2 cut(s) 249, 367
MvaI CCWGG 1 cut(s) 238
MvnI CGCG 1 cut(s) 207
MwoI GCNNNNNNNGC 5 cut(s) 175, 638, 926, 1068, 1124
NaeI GCCGGC 1 cut(s) 73
NarI GGCGCC 1 cut(s) 49
NdeII GATC 8 cut(s) 208, 469, 473, 552, 1032, 1088, 1130, 1138
NgoMIV GCCGGC 1 cut(s) 71
NlaIII CATG 4 cut(s) 544, 645, 1033, 1145
NlaIV GGNNCC 2 cut(s) 50, 961
NmuCI GTSAC 1 cut(s) 613
NspV TTCGAA 2 cut(s) 187, 1150
OliI CACNNNNGTG 1 cut(s) 992
PaeR7I CTCGAG 1 cut(s) 873
PceI AGGCCT 1 cut(s) 275
PctI GAATGC 2 cut(s) 249, 367
PdiI GCCGGC 1 cut(s) 73
PfeI GAWTC 5 cut(s) 324, 337, 354, 593, 1190
PflFI GACNNNGTC 1 cut(s) 425
PflMI CCANNNNNTGG 1 cut(s) 243
PkrI GCNGC 8 cut(s) 36, 203, 206, 517, 634, 826, 928, 931
Ple19I CGATCG 1 cut(s) 555
PleI GAGTC 2 cut(s) 304, 766
PluTI GGCGCC 1 cut(s) 52
PpsI GAGTC 2 cut(s) 304, 766
PshBI ATTAAT 2 cut(s) 104, 510
Psp124BI GAGCTC 2 cut(s) 43, 748
Psp6I CCWGG 1 cut(s) 236
PspGI CCWGG 1 cut(s) 236
PspN4I GGNNCC 2 cut(s) 50, 961
PstI CTGCAG 1 cut(s) 1129
PstNI CAGNNNCTG 1 cut(s) 1133
PsuI RGATCY 1 cut(s) 1130
PsyI GACNNNGTC 1 cut(s) 425
PvuI CGATCG 1 cut(s) 555
PvuII CAGCTG 1 cut(s) 929
RsaI GTAC 2 cut(s) 302, 953
RsaNI GTAC 2 cut(s) 301, 952
RseI CAYNNNNRTG 3 cut(s) 640, 992, 1110
SacI GAGCTC 2 cut(s) 43, 748
SalI GTCGAC 1 cut(s) 118
SaqAI TTAA 3 cut(s) 104, 510, 1046
SatI GCNGC 8 cut(s) 35, 202, 205, 516, 633, 825, 927, 930
Sau3AI GATC 8 cut(s) 208, 469, 473, 552, 1032, 1088, 1130, 1138
SchI GAGTC 2 cut(s) 304, 767
ScrFI CCNGG 1 cut(s) 238
SduI GDGCHC 3 cut(s) 43, 748, 989
SfaNI GCATC 3 cut(s) 644, 705, 1105
SfcI CTRYAG 1 cut(s) 1125
SfoI GGCGCC 1 cut(s) 50
Sfr274I CTCGAG 1 cut(s) 873
SfuI TTCGAA 2 cut(s) 187, 1150
SgrAI CRCCGGYG 1 cut(s) 71
SlaI CTCGAG 1 cut(s) 873
SmiMI CAYNNNNRTG 3 cut(s) 640, 992, 1110
SmlI CTYRAG 4 cut(s) 755, 873, 1038, 1045
SmoI CTYRAG 4 cut(s) 755, 873, 1038, 1045
Sse9I AATT 8 cut(s) 19, 28, 241, 618, 750, 816, 879, 939
SseBI AGGCCT 1 cut(s) 275
SsiI CCGC 8 cut(s) 69, 205, 289, 408, 661, 695, 824, 1213
SspDI GGCGCC 1 cut(s) 48
SspMI CTAG 1 cut(s) 1005
SstI GAGCTC 2 cut(s) 43, 748
StuI AGGCCT 1 cut(s) 275
StyD4I CCNGG 1 cut(s) 236
StyI CCWWGG 1 cut(s) 1084
TaaI ACNGT 4 cut(s) 117, 165, 994, 1109
TaiI ACGT 2 cut(s) 581, 957
TaqI TCGA 5 cut(s) 119, 187, 472, 874, 1150
TasI AATT 8 cut(s) 19, 28, 241, 618, 750, 816, 879, 939
TatI WGTACW 1 cut(s) 300
TauI GCSGC 2 cut(s) 207, 827
TfiI GAWTC 5 cut(s) 324, 337, 354, 593, 1190
Tru1I TTAA 3 cut(s) 104, 510, 1046
Tru9I TTAA 3 cut(s) 104, 510, 1046
TscAI CASTG 2 cut(s) 999, 1114
TseFI GTSAC 1 cut(s) 613
TseI GCWGC 6 cut(s) 34, 201, 515, 632, 926, 929
Tsp45I GTSAC 1 cut(s) 613
TspDTI ATGAA 2 cut(s) 681, 921
TspGWI ACGGA 4 cut(s) 13, 132, 138, 330
TspRI CASTG 2 cut(s) 999, 1114
Tth111I GACNNNGTC 1 cut(s) 425
Van91I CCANNNNNTGG 1 cut(s) 243
Vha464I CTTAAG 1 cut(s) 1045
VspI ATTAAT 2 cut(s) 104, 510
XapI RAATTY 3 cut(s) 19, 750, 879
XhoI CTCGAG 1 cut(s) 873
XmiI GTMKAC 1 cut(s) 119
XspI CTAG 1 cut(s) 1005
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.