pycom01g23850

Stomatin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0004436
Physical Location & Seq
Forward (+)
21034217 .. 21037245
3029 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g23850.6

Sequence Viewer

Length: 1224 bp
ATGAGTATGTTCAAGCTCAATTCCCTCAACTCGCTGCGAGCTCTGAGGCACCTCCACGCTTCCCAATCCGCCGGCGTCAGAGCGCTCACCACCTGCTCGTCATTAATCCTCAAACAGTCGAACCCACTTCTCTCCCCCTCTCCGTCGCCGTCTCCCTTCGCACCGTCGCACAATCTGCTCGCCATTCGGAACTACCGGAGCAGCCGCGATCTCAGAAACTATGGGATTACGCCTCCCAAGAATTGGGGCATTCGGATTGTGCCTGAGAAGAAGGCCTTTGTGATTGAGCGGTTCGGAAAGTATGTCAAGACTCTGCCTTCTGGAATCCATTTCTTGATTCCGTTTGTGGATAGAATAGCTTATGTGCATTCACTGAAAGAAGAGGTCATCAATGTTCCTGACCAGTCTGCCATTACCAAAGACAATGTCGCCCTTATGATTGATGGTGTGCTTTACGTCAGGATTGTGGATCCTATGCTGGCCTCCTATGGAGTTGACGATCCTATTAATGCAGCTATTCAGCTTGCACAGACTACCATGCGTATTGCTATCGGGAAAATCACCCTTGACAAGACATTTGAGGAAAGGGAATCCCTCAATGATGAGATAGTGACAGTTCTCAACAAGGCTGCGTCAGCTTGGGGGTTGGAGTGCCTCCGCTATGAAATCAAGGACATAACTCCTCCTCGTGCGGTGAGGGAATCTATGGAGATGCAAGCAGAAGCAGAACGCAAAAAGAGAGCTCAAATTCTTGAGTCTGAGGGAGAAAGGCAGGCCAACATAAATATTGCAGATGGAAAGAAGAGTGCTATAATTTTAGCAGCAGAAGGAGAAGCGCAATCCATTCTTGCTAAAGCGAATGCAACATCTCGAGGAATTTCCCTTGTGTCAAAGACTCTCAAGGAAAGTGGGGGTGCAGAGGCAGCTGCTTTGAAAATTGCTGAGCAGTATGTTGATGCCTTTGGTAATATCGCGAAACAGGGCACAACAGTGTTGCTTCCTAGTGCTTCAAATAATCCTGCTGGCATGATATCTCAAGCCTTAAGTATCTACAAAAGCTTGGTTAACAATCCTTCCACCCTTGGATCAACTGGAGGCACGCACAGTGATGATGCTTCCTCAGAAGGATCTGATCACGGTGTTCGAATGATCGAGGAGACAAAGAATCAAGATAATGCGAAGGAATCAGGATTTTCGCTTCAGAACCGCAAAAAAGGAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006996 GO:0007005 GO:0007006 GO:0008150 GO:0009987 GO:0016043 GO:0061024 GO:0071840
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Band_7 Band_7_C

Protein Analysis

408

Amino Acids

44.14

Weight (kDa)

9.08

Isoelectric Point (pI)

45.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 101
Acc36I ACCTGC 1 cut(s) 101
AccB1I GGYRCC 1 cut(s) 48
AccB7I CCANNNNNTGG 1 cut(s) 243
AccBSI CCGCTC 1 cut(s) 289
AccII CGCG 2 cut(s) 207, 974
AciI CCGC 6 cut(s) 69, 205, 289, 658, 692, 1207
AclWI GGATC 5 cut(s) 464, 477, 494, 1093, 1135
AcsI RAATTY 2 cut(s) 747, 876
AcuI CTGAAG 1 cut(s) 1184
AcyI GRCGYC 1 cut(s) 75
AfeI AGCGCT 1 cut(s) 84
AfiI CCNNNNNNNGG 1 cut(s) 243
AflII CTTAAG 1 cut(s) 1042
AgsI TTSAA 3 cut(s) 13, 934, 1011
AleI CACNNNNGTG 1 cut(s) 989
AluBI AGCT 9 cut(s) 16, 41, 359, 515, 523, 638, 743, 926, 1059
AluI AGCT 9 cut(s) 16, 41, 359, 515, 523, 638, 743, 926, 1059
Alw21I GWGCWC 2 cut(s) 43, 745
Alw26I GTCTC 2 cut(s) 156, 1151
AlwI GGATC 5 cut(s) 464, 477, 494, 1093, 1135
Ama87I CYCGRG 1 cut(s) 870
Aor51HI AGCGCT 1 cut(s) 84
AoxI GGCC 3 cut(s) 273, 480, 774
ApeKI GCWGC 7 cut(s) 34, 201, 512, 629, 821, 923, 926
ApoI RAATTY 2 cut(s) 747, 876
ArsI GACNNNNNNTTYG 4 cut(s) 411, 443, 560, 592
AseI ATTAAT 2 cut(s) 104, 507
AspLEI GCGC 2 cut(s) 85, 838
AsuHPI GGTGA 3 cut(s) 79, 553, 706
AsuII TTCGAA 1 cut(s) 1144
AvaI CYCGRG 1 cut(s) 870
BaeGI GKGCMC 1 cut(s) 986
BamHI GGATCC 1 cut(s) 469
BanI GGYRCC 1 cut(s) 48
BanII GRGCYC 2 cut(s) 43, 745
BauI CACGAG 1 cut(s) 687
Bbv12I GWGCWC 2 cut(s) 43, 745
BbvI GCAGC 7 cut(s) 21, 213, 524, 616, 833, 913, 935
BccI CCATC 2 cut(s) 437, 788
BceAI ACGGC 1 cut(s) 133
BclI TGATCA 1 cut(s) 1132
BcoDI GTCTC 2 cut(s) 156, 1151
BfaI CTAG 1 cut(s) 1002
BfoI RGCGCY 1 cut(s) 86
BfrI CTTAAG 1 cut(s) 1042
BfuAI ACCTGC 1 cut(s) 101
BisI GCNGC 8 cut(s) 35, 202, 205, 513, 630, 822, 924, 927
BlpI GCTNAGC 1 cut(s) 942
BlsI GCNGC 8 cut(s) 36, 203, 206, 514, 631, 823, 925, 928
BmeT110I CYCGRG 1 cut(s) 870
BmiI GGNNCC 2 cut(s) 50, 471
BmsI GCATC 3 cut(s) 702, 946, 1102
BpmI CTGGAG 1 cut(s) 1113
Bpu1102I GCTNAGC 1 cut(s) 942
Bpu14I TTCGAA 1 cut(s) 1144
BpuEI CTTGAG 3 cut(s) 773, 884, 1020
BsaHI GRCGYC 1 cut(s) 75
BsaJI CCNNGG 1 cut(s) 1081
BsaWI WCCGGW 1 cut(s) 195
Bsc4I CCNNNNNNNGG 1 cut(s) 243
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 2 cut(s) 403, 1096
BseDI CCNNGG 1 cut(s) 1081
BseLI CCNNNNNNNGG 1 cut(s) 243
BseMII CTCAG 6 cut(s) 35, 226, 255, 750, 933, 1134
BseNI ACTGG 2 cut(s) 403, 1096
BseRI GAGGAG 3 cut(s) 672, 675, 1169
BseSI GKGCMC 1 cut(s) 986
BseXI GCAGC 7 cut(s) 21, 213, 524, 616, 833, 913, 935
BsgI GTGCAG 1 cut(s) 936
Bsh1236I CGCG 2 cut(s) 207, 974
BshFI GGCC 3 cut(s) 275, 482, 776
BshNI GGYRCC 1 cut(s) 48
BsiHKAI GWGCWC 2 cut(s) 43, 745
BsiHKCI CYCGRG 1 cut(s) 870
BsiSI CCGG 2 cut(s) 72, 196
BslI CCNNNNNNNGG 1 cut(s) 243
BsmAI GTCTC 2 cut(s) 156, 1151
BsmBI CGTCTC 1 cut(s) 156
BsmI GAATGC 3 cut(s) 249, 367, 865
BsnI GGCC 3 cut(s) 275, 482, 776
BsoBI CYCGRG 1 cut(s) 870
Bsp119I TTCGAA 1 cut(s) 1144
Bsp1286I GDGCHC 3 cut(s) 43, 745, 986
Bsp143I GATC 7 cut(s) 208, 469, 499, 1085, 1127, 1132, 1149
Bsp1720I GCTNAGC 1 cut(s) 942
Bsp68I TCGCGA 1 cut(s) 974
BspACI CCGC 6 cut(s) 69, 205, 289, 658, 692, 1207
BspANI GGCC 3 cut(s) 275, 482, 776
BspCNI CTCAG 6 cut(s) 36, 225, 256, 751, 934, 1133
BspFNI CGCG 2 cut(s) 207, 974
BspLI GGNNCC 2 cut(s) 50, 471
BspMI ACCTGC 1 cut(s) 101
BspPI GGATC 5 cut(s) 464, 477, 494, 1093, 1135
BspT104I TTCGAA 1 cut(s) 1144
BspT107I GGYRCC 1 cut(s) 48
BspTI CTTAAG 1 cut(s) 1042
BsrBI CCGCTC 1 cut(s) 289
BsrFI RCCGGY 1 cut(s) 71
BsrI ACTGG 2 cut(s) 403, 1096
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 1 cut(s) 1081
BssMI GATC 7 cut(s) 208, 469, 499, 1085, 1127, 1132, 1149
BssNI GRCGYC 1 cut(s) 75
BssSI CACGAG 1 cut(s) 687
BssT1I CCWWGG 1 cut(s) 1081
Bst2BI CACGAG 1 cut(s) 687
Bst4CI ACNGT 6 cut(s) 117, 165, 616, 991, 1106, 1139
Bst6I CTCTTC 2 cut(s) 375, 797
BstACI GRCGYC 1 cut(s) 75
BstAFI CTTAAG 1 cut(s) 1042
BstAPI GCANNNNNTGC 1 cut(s) 175
BstBI TTCGAA 1 cut(s) 1144
BstC8I GCNNGC 9 cut(s) 39, 73, 180, 480, 525, 717, 774, 1024, 1100
BstDEI CTNAG 6 cut(s) 44, 212, 264, 759, 942, 1120
BstFNI CGCG 2 cut(s) 207, 974
BstH2I RGCGCY 1 cut(s) 86
BstHHI GCGC 2 cut(s) 85, 838
BstKTI GATC 7 cut(s) 211, 472, 502, 1088, 1130, 1135, 1152
BstMAI GTCTC 2 cut(s) 156, 1151
BstMBI GATC 7 cut(s) 208, 469, 499, 1085, 1127, 1132, 1149
BstMWI GCNNNNNNNGC 3 cut(s) 175, 635, 923
BstSLI GKGCMC 1 cut(s) 986
BstUI CGCG 2 cut(s) 207, 974
BstV1I GCAGC 7 cut(s) 21, 213, 524, 616, 833, 913, 935
BstX2I RGATCY 2 cut(s) 469, 1127
BstYI RGATCY 2 cut(s) 469, 1127
BsuRI GGCC 3 cut(s) 275, 482, 776
BtsIMutI CAGTG 3 cut(s) 371, 996, 1111
BtuMI TCGCGA 1 cut(s) 974
BveI ACCTGC 1 cut(s) 101
Cac8I GCNNGC 9 cut(s) 39, 73, 180, 480, 525, 717, 774, 1024, 1100
CfoI GCGC 2 cut(s) 85, 838
Cfr10I RCCGGY 1 cut(s) 71
CseI GACGC 2 cut(s) 64, 621
CspCI CAANNNNNGTGG 2 cut(s) 889, 924
CviAII CATG 2 cut(s) 538, 1027
DdeI CTNAG 6 cut(s) 44, 212, 264, 759, 942, 1120
DpnI GATC 7 cut(s) 210, 471, 501, 1087, 1129, 1134, 1151
DpnII GATC 7 cut(s) 208, 469, 499, 1085, 1127, 1132, 1149
Eam1104I CTCTTC 2 cut(s) 375, 797
EarI CTCTTC 2 cut(s) 375, 797
EciI GGCGGA 1 cut(s) 58
Ecl136II GAGCTC 2 cut(s) 41, 743
Eco130I CCWWGG 1 cut(s) 1081
Eco147I AGGCCT 1 cut(s) 275
Eco24I GRGCYC 2 cut(s) 43, 745
Eco32I GATATC 1 cut(s) 1032
Eco47III AGCGCT 1 cut(s) 84
Eco53kI GAGCTC 2 cut(s) 41, 743
Eco57I CTGAAG 1 cut(s) 1184
Eco88I CYCGRG 1 cut(s) 870
EcoICRI GAGCTC 2 cut(s) 41, 743
EcoRV GATATC 1 cut(s) 1032
EcoT14I CCWWGG 1 cut(s) 1081
EcoT38I GRGCYC 2 cut(s) 43, 745
ErhI CCWWGG 1 cut(s) 1081
Esp3I CGTCTC 1 cut(s) 156
FaeI CATG 2 cut(s) 541, 1030
FalI AAGNNNNNCTT 2 cut(s) 260, 292
FatI CATG 2 cut(s) 537, 1026
FbaI TGATCA 1 cut(s) 1132
Fnu4HI GCNGC 8 cut(s) 35, 202, 205, 513, 630, 822, 924, 927
FriOI GRGCYC 2 cut(s) 43, 745
Fsp4HI GCNGC 8 cut(s) 35, 202, 205, 513, 630, 822, 924, 927
FspBI CTAG 1 cut(s) 1002
GlaI GCGC 2 cut(s) 84, 837
GluI GCNGC 8 cut(s) 35, 202, 205, 513, 630, 822, 924, 927
GsuI CTGGAG 1 cut(s) 1113
HaeII RGCGCY 1 cut(s) 86
HaeIII GGCC 3 cut(s) 275, 482, 776
HapII CCGG 2 cut(s) 72, 196
HgaI GACGC 2 cut(s) 64, 621
HhaI GCGC 2 cut(s) 85, 838
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 2 cut(s) 541, 1030
Hin6I GCGC 2 cut(s) 83, 836
HinP1I GCGC 2 cut(s) 83, 836
HincII GTYRAC 2 cut(s) 496, 1066
HindII GTYRAC 2 cut(s) 496, 1066
HindIII AAGCTT 1 cut(s) 1057
HinfI GANTC 9 cut(s) 310, 324, 337, 590, 701, 755, 895, 1165, 1184
HpaI GTTAAC 1 cut(s) 1066
HpaII CCGG 2 cut(s) 72, 196
HphI GGTGA 3 cut(s) 79, 553, 706
Hpy166II GTNNAC 2 cut(s) 496, 1066
Hpy8I GTNNAC 2 cut(s) 496, 1066
Hpy99I CGWCG 2 cut(s) 148, 169
HpyAV CCTTC 7 cut(s) 166, 265, 327, 821, 1083, 1118, 1174
HpyCH4III ACNGT 6 cut(s) 117, 165, 616, 991, 1106, 1139
HpyCH4IV ACGT 1 cut(s) 456
HpyCH4V TGCA 7 cut(s) 367, 512, 527, 715, 791, 863, 917
HpyF10VI GCNNNNNNNGC 3 cut(s) 175, 635, 923
HpyF3I CTNAG 6 cut(s) 44, 212, 264, 759, 942, 1120
HpySE526I ACGT 1 cut(s) 456
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 2 cut(s) 541, 1030
HspAI GCGC 2 cut(s) 83, 836
KroI GCCGGC 1 cut(s) 71
KroNI GCCGGC 1 cut(s) 73
Ksp22I TGATCA 1 cut(s) 1132
KspAI GTTAAC 1 cut(s) 1066
Kzo9I GATC 7 cut(s) 208, 469, 499, 1085, 1127, 1132, 1149
LmnI GCTCC 2 cut(s) 198, 1217
Lsp1109I GCAGC 7 cut(s) 21, 213, 524, 616, 833, 913, 935
LweI GCATC 3 cut(s) 702, 946, 1102
MaeI CTAG 1 cut(s) 1002
MaeII ACGT 1 cut(s) 456
MaeIII GTNAC 1 cut(s) 610
MalI GATC 7 cut(s) 210, 471, 501, 1087, 1129, 1134, 1151
MbiI CCGCTC 1 cut(s) 289
MboI GATC 7 cut(s) 208, 469, 499, 1085, 1127, 1132, 1149
MboII GAAGA 3 cut(s) 280, 392, 814
MflI RGATCY 2 cut(s) 469, 1127
MhlI GDGCHC 3 cut(s) 43, 745, 986
MluCI AATT 6 cut(s) 19, 241, 747, 813, 876, 936
MlyI GAGTC 3 cut(s) 304, 764, 889
MmeI TCCRAC 1 cut(s) 627
MreI CGCCGGCG 1 cut(s) 71
MroNI GCCGGC 1 cut(s) 71
MseI TTAA 4 cut(s) 104, 507, 1043, 1065
MslI CAYNNNNRTG 2 cut(s) 989, 1107
MspA1I CMGCKG 1 cut(s) 926
MspCI CTTAAG 1 cut(s) 1042
MspI CCGG 2 cut(s) 72, 196
Mva1269I GAATGC 3 cut(s) 249, 367, 865
MvnI CGCG 2 cut(s) 207, 974
MwoI GCNNNNNNNGC 3 cut(s) 175, 635, 923
NaeI GCCGGC 1 cut(s) 73
NdeII GATC 7 cut(s) 208, 469, 499, 1085, 1127, 1132, 1149
NgoMIV GCCGGC 1 cut(s) 71
NlaIII CATG 2 cut(s) 541, 1030
NlaIV GGNNCC 2 cut(s) 50, 471
NmuCI GTSAC 1 cut(s) 610
NruI TCGCGA 1 cut(s) 974
NspV TTCGAA 1 cut(s) 1144
OliI CACNNNNGTG 1 cut(s) 989
PaeR7I CTCGAG 1 cut(s) 870
PaqCI CACCTGC 1 cut(s) 101
PceI AGGCCT 1 cut(s) 275
PctI GAATGC 3 cut(s) 249, 367, 865
PdiI GCCGGC 1 cut(s) 73
PfeI GAWTC 6 cut(s) 324, 337, 590, 701, 1165, 1184
PflFI GACNNNGTC 1 cut(s) 425
PflMI CCANNNNNTGG 1 cut(s) 243
PkrI GCNGC 8 cut(s) 36, 203, 206, 514, 631, 823, 925, 928
PleI GAGTC 3 cut(s) 304, 763, 889
PpsI GAGTC 3 cut(s) 304, 763, 889
PshBI ATTAAT 2 cut(s) 104, 507
Psp124BI GAGCTC 2 cut(s) 43, 745
PspN4I GGNNCC 2 cut(s) 50, 471
PsuI RGATCY 2 cut(s) 469, 1127
PsyI GACNNNGTC 1 cut(s) 425
PvuII CAGCTG 1 cut(s) 926
RruI TCGCGA 1 cut(s) 974
RseI CAYNNNNRTG 2 cut(s) 989, 1107
SacI GAGCTC 2 cut(s) 43, 745
SaqAI TTAA 4 cut(s) 104, 507, 1043, 1065
SatI GCNGC 8 cut(s) 35, 202, 205, 513, 630, 822, 924, 927
Sau3AI GATC 7 cut(s) 208, 469, 499, 1085, 1127, 1132, 1149
SchI GAGTC 3 cut(s) 304, 764, 889
SduI GDGCHC 3 cut(s) 43, 745, 986
SfaNI GCATC 3 cut(s) 702, 946, 1102
Sfr274I CTCGAG 1 cut(s) 870
SfuI TTCGAA 1 cut(s) 1144
SgrAI CRCCGGYG 1 cut(s) 71
SlaI CTCGAG 1 cut(s) 870
SmiMI CAYNNNNRTG 2 cut(s) 989, 1107
SmlI CTYRAG 5 cut(s) 752, 870, 899, 1035, 1042
SmoI CTYRAG 5 cut(s) 752, 870, 899, 1035, 1042
Sse9I AATT 6 cut(s) 19, 241, 747, 813, 876, 936
SseBI AGGCCT 1 cut(s) 275
SsiI CCGC 6 cut(s) 69, 205, 289, 658, 692, 1207
SspI AATATT 1 cut(s) 787
SspMI CTAG 1 cut(s) 1002
SstI GAGCTC 2 cut(s) 43, 745
StuI AGGCCT 1 cut(s) 275
StyI CCWWGG 1 cut(s) 1081
TaaI ACNGT 6 cut(s) 117, 165, 616, 991, 1106, 1139
TaiI ACGT 1 cut(s) 459
TaqI TCGA 4 cut(s) 119, 871, 1144, 1152
TasI AATT 6 cut(s) 19, 241, 747, 813, 876, 936
TauI GCSGC 1 cut(s) 207
TfiI GAWTC 6 cut(s) 324, 337, 590, 701, 1165, 1184
Tru1I TTAA 4 cut(s) 104, 507, 1043, 1065
Tru9I TTAA 4 cut(s) 104, 507, 1043, 1065
TscAI CASTG 3 cut(s) 378, 996, 1111
TseFI GTSAC 1 cut(s) 610
TseI GCWGC 7 cut(s) 34, 201, 512, 629, 821, 923, 926
Tsp45I GTSAC 1 cut(s) 610
TspDTI ATGAA 1 cut(s) 678
TspGWI ACGGA 2 cut(s) 132, 330
TspRI CASTG 3 cut(s) 378, 996, 1111
Tth111I GACNNNGTC 1 cut(s) 425
Van91I CCANNNNNTGG 1 cut(s) 243
Vha464I CTTAAG 1 cut(s) 1042
VspI ATTAAT 2 cut(s) 104, 507
XapI RAATTY 2 cut(s) 747, 876
XhoI CTCGAG 1 cut(s) 870
XspI CTAG 1 cut(s) 1002
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.