Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0002550

Physical Location & Seq

Genomic Coordinates Forward (+)

32189437 .. 32197045

Gene Structure 7609 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1234100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3408 bp

ATGTCTTCGTTAAGCAGGGAGCTAGTATTCCTGATCTTACAGTTCTTAGATGAGGAGAAGTTCAAGGAAACAGTTCATAGGCTTGAGCAAGAGTCTGGTTTTTTCTTCAATATGAAGCATTTCGAGGACCAAGTCCAGGCTGGAGAGTGGGATGAAGTTGAGCGCTATTTGTGTGGCTTCACTAAAGTTGAAGACAACCGGTATTCAATGAAGATTTTTTTTGAAATTAGGAAGCAGAAGTATTTGGAAGCTCTTGACAGGCAGGATAGGGCAAGGGCTGTTGAGATTCTTGTGAAGGACCTTAAGGTTTTTTCATCATTTAATGAGGAACTTTTCAAGGAGATCACCCAGTTGCTTAGTCTTGATAATTTTAGGCAAAATGAGCAGCTTTCCAAGTATGGAGACACAAAATCGGCTCGAAATATCATGCTTATAGAACTTAAAAAGCTCATTGAGGCGAACCCATTATTTCGTGATAAGCTTGCATTCCCAGCCTTCAAAAGTTCGCGTCTTCGAACGTTAATAAACCAGAGTCTCAATTGGCAGCACCAGCTTTGCAAGAATCCCCGTCCAAACCCTGATATTAAAACACTATTTATGGATCATTCTTGCACTCCAACTGCTAACGGATCTCGTCCACCACCCACAAATAGTCCCCTTGTAGGACCAATTCCAAAGGCTGGCGCATTTCCTCCTATTGGTGCCCATGGTCCATTTCAACCCGTTGTCTCCCCATCTCCTGGTGCCATTGCTGGGTGGATGTCAAGCACTAATCCTTCTTTGCCCCACCCTGCTGTTCCAGCAGCTCCTCCTGGTATGCAGCCTTCTAGTGCAGCTGCTTTCTTGAAGCACCCAAGGACCCCTACAGGTGTTACAGGGATTGATTATCAATCAGCTGATTCAGAGCACTTGATGAAGCGCATTCGCACTGGCCAAGCTGATGAGGTGTCCTTTTCTGGTGTGATGCATAATTCCAATGTCTATTCGCAAGATGATCTTCCTAGGGCTGTTGTCCGTACCCTTAGTCAAGGATCTAATGTGATGAGCATGGACTTCCATCCACAACAGCAAACTATTCTTCTAGTTGGAACAAATGTTGGTGATATCAGCCTCTGGGAAGTAGGGTCCCGGGAGAGGTTGGTACATAGGCCTTTCAAGGTTTGGGATATACAAACTGCGTCAACACCATTGCAGACAGCTCTGGTGAATGATGCTGCAATATCAGTGAATCGTTGTGTATGGGCACCAGATGGACTTATGTTGGGTGTTGCATTCTCTAAGCACATTGTTCAGATATATAATTACAATCCAACTGGAGAACTGAGACAACATTTGGAGATTGATGCTCATGTCGGCGGTGTTAATGACATTGCATTCGCTCATCCCAACAAGCAACTGTGTATTGTCACGTGTGGAGATGATAGAGTGATTAAGGTATGGGATGCTGTAGCCGGACGCAGACAGTATACATTTGAAGGCCATGAAGCTCCTGTGTATTCAGTGTGCCCTCACTACAAAGAAAATATCCAGTTCATTTTTTCAACTGCCATTGACGGGAAGATTAAAGCTTGGCTATATGATTGCTTGGGATCACGAGTGGACTATGATGCTCCTGGACTTTGGTGCACTATGATGTTGTATAGTGCAGATGGAACCAGACTCTTTTCCTGTGGAACAAGTAAAGACGGTGAATCCCATCTGGTGGAGTGGAATGAGAGTGAAGGGGCTATCAAACGGACATATTCTGGTTTTAGAAAACGCTGTTTAGATGTTGTTCAGTTTGACACAACAAGGAACCGATTCTTAGCTGCTGGTGATGAATTTCAAATTAAGTTCTGGGATATGGACAATACCAATGTGTTGGCAGCTGTGGATGCAGATGGCGGTTTGCCTGCTAGTCCTAGGCTGAGATTCAATAAAGAAGGGTCACTGTTGGCTGTAACAACGAATGACAGTGGTATTAAGATTTTAGCAAATAATGATGGCTTGCGCTTGATTAGAATGTTAGAGGGAAGGGCTATGGAGAAAAATCGAGGCACTTCTGAGCCCATCAACTCTAAGCCTCTGATGGTTAATGCAATAGGTGCCATTGTGAATGTTCCCAACGCTGTTGTTCCATCCCTGGAACGCCCTGATAGAATACAACCAGTTGTGTCTATCAGTAATCTAGGCACTATGGAGAACAGCAGGCTTGTTGATGTTAAACCACGGATTTCTGATGATATAGACAAAATTAAGAGCTGGAAATTTTCTGATGTTGCGGATCCCTCTCAAATGAAAGCTCTGCGACTGCCCGACTCTACAACTGCTGGAAAGTTGCAGATTGTCAGACTAATTTACACCAACAATGGCCTGGCTCTGTTAGCCCTTGCCTCCAATGCTGTTCACAAGCTCTGGAAATGGCAGCGGAATGAAAGGAATCCATCCGGGAAGGCTAGTGCATACGTTGCACCACAGTTGTGGCAGCCACCCAATGGAACTCTTATGACTAATGATGTAAATGACAATAAACCAGCTGAGGAATCTATTGCGTGCATTGCTTTATCCAAAAATGATTCTTACGTCATGTCTGCATCTGGTGGAAAGGTCTCTTTGTTCAACATGATGACATTTAAGGTCATGACAACATTTGTATCTCCCCCTCCCGCTGCCACTTTTTTGGCATTTCATCCTCAAGACAATAATATAATTGCAATTGGCATGGAGGATTCTACTATATTAATTTATAATGTTAGGGTTGATGAGGTCAAAACCAAACTGAAAGGTCACCAGAATCGGATTACAGGCCTTGCATTTTCCCAAATTCTTAATGTACTGGTATCTTCAGGTGCTGATACCCAGTTATGTGTGTGGAGCATTGATGGATGGGAGAAAAAGAAAACAAGGTTCATACAAGCACCAGCTGGTCGTCAACCCCCGATGGTTGGAGAAACAAAAGTTCAGTTTCATAATGATCACACACACCTGTTGGTTGCCCATGAAAGCCAAGTTGCTGTGTACGATTGCAAGCTTGAATGTTTGCGCTCATGGTCTCCGAAAGATGCACTCGCTGCTCCCATATCAAGTGCAATATATTCATGTGATGGTCTACTGGTTTATGCTTCATTTTGTGACGGTGCTGTTGGAGTTTTTGATGCTGATACCTTAAGACTCAGATGTCGAGTAGCACCTACTGCTTATATACCATCATTTTCTCTCAGCGGAAACCCCAGCTACCCTTTGGTTATAGCAGCTCATCCATCTGAGCCGAATCAGATTGCACTTGGCATGACTGACGGCTCAGTGCATGTAGTTGAACCGTCTGATGTAGAGCTGAAGTGGGGTGGGGCGCCCCCTCTAGAGAATGGCCCTTCCAATTCGTCAAATCCCTCTCCTAGCGGTCAAGCATCTGAACATCCTTCCCGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003002 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009753 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009867 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009933 GO:0009987 GO:0010014 GO:0010016 GO:0010033 GO:0010051 GO:0010072 GO:0010154 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032870 GO:0042221 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045892 GO:0045934 GO:0046983 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048519 GO:0048523 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0080090 GO:0090421 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

1136

Amino Acids

125.62

Weight (kDa)

6.75

Isoelectric Point (pI)

39.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LisH_TPL	PF17814	5 - 34	2.5e-12	LisH-like dimerisation domain
TPR1-like_2nd	PF21889	37 - 161	3.6e-61	TPR1-like, CTLH-containing domain
zf_topless	PF21359	164 - 204	1.6e-25	TOPLESS, zinc finger domain
WD40_CDC20-Fz	PF24807	326 - 613	3.1e-09	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd	PF25172	345 - 559	6.8e-07	WDR3 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	386 - 524	2.1e-13	WDR3 first beta-propeller domain
WD40_Gbeta	PF25391	406 - 622	1.3e-07	G protein beta WD-40 repeat protein
Beta-prop_THOC3	PF25174	408 - 666	1.6e-20	THOC3 beta-propeller domain
Beta-prop_WDR5	PF25175	409 - 620	6.1e-19	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	422 - 525	3.8e-09	WDHD1 first WD40 domain
WD40_Prp19	PF24814	451 - 524	2.3e-06	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	566 - 672	8.2e-06	WDR36/Utp21 second beta-propeller domain
WD40_Prp19	PF24814	818 - 1003	7.6e-14	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	833 - 958	1.3e-13	CDC20/Fizzy WD40 domain
WD40_WDHD1_1st	PF24817	836 - 1049	3.5e-18	WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	836 - 954	3e-10	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	837 - 955	6.7e-17	WDR3 first beta-propeller domain
Beta-prop_EML_2	PF23414	838 - 959	1.6e-10	Echinoderm microtubule-associated protein second beta-propeller
WDR55	PF24796	838 - 954	4e-06	WDR55
Beta-prop_WDR5	PF25175	840 - 1048	1.3e-16	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	843 - 961	3.2e-12	WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	856 - 955	4e-10	WDR36/Utp21 first beta-propeller
Beta-prop_IFT140_1st	PF23383	856 - 956	6.4e-11	IFT140 first beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	861 - 959	4.7e-08	WDR90/POC16, second beta-propeller
Beta-prop_RIG_1st	PF23770	870 - 937	1.4e-07	RIG first beta-propeller
WD40_Prp19	PF24814	881 - 1053	6e-14	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	917 - 1097	6.2e-06	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002550)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770
malus_domestica	MD01G1234100.v1.1 MD07G1306300.v1.1
prunus_persica	Prupe.2G324300_v2.0.a1
pyrus_communis	pycom01g24260 pycom07g27930
rosa_chinensis	RchiOBHm_Chr1g0382471 RchiOBHm_Chr1g0382481 RchiOBHm_Chr1g0383911
rosa_laevigata	RLG00000026087 RLG00000026174 RLG00000026177
rosa_multiflora	Rmu_sc0000110.1_g000051 Rmu_sc0003885.1_g000009 Rmu_sc0005139.1_g000028
rosa_roxburghii	Rroxscaffold_4G00277160 Rroxscaffold_4G00278020 Rroxscaffold_4G00278080
rosa_rugosa	Rorug01G0434600 Rorug01G0434900 Rorug01G0443000
rosa_samantha	Rh1AG459200 Rh1AG459300 Rh1AG459600 Rh1AG468900 Rh1BG415700 Rh1BG426600 Rh1CG429700 Rh1CG429800 Rh1CG439400 Rh1DG447800 Rh1DG447900 Rh1DG459400
rosa_wichuraiana	Rw1G040350 Rw1G041180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2727
AasI	GACNNNNNNGTC	1 cut(s)	3156
AccB1I	GGYRCC	5 cut(s)	701, 743, 1243, 2084, 3328
AccB7I	CCANNNNNTGG	4 cut(s)	680, 740, 1702, 2474
AccI	GTMKAC	2 cut(s)	1466, 3088
AccII	CGCG	1 cut(s)	508
AciI	CCGC	7 cut(s)	1356, 1884, 2261, 2407, 2646, 3201, 3378
AclI	AACGTT	1 cut(s)	518
AclWI	GGATC	6 cut(s)	609, 637, 1039, 1597, 2258, 2271
AcoI	YGGCCR	1 cut(s)	931
AcsI	RAATTY	3 cut(s)	1820, 2246, 2802
AcuI	CTGAAG	2 cut(s)	2808, 3335
AcvI	CACGTG	1 cut(s)	1410
AcyI	GRCGYC	1 cut(s)	3329
AfaI	GTAC	4 cut(s)	1018, 1143, 2814, 2999
AfeI	AGCGCT	1 cut(s)	164
AflII	CTTAAG	2 cut(s)	302, 3145
AflIII	ACRYGT	1 cut(s)	1409
AgeI	ACCGGT	1 cut(s)	198
AjnI	CCWGG	6 cut(s)	135, 739, 811, 1612, 2121, 2352
AleI	CACNNNNGTG	1 cut(s)	2458
Alw21I	GWGCWC	2 cut(s)	909, 1628
Alw26I	GTCTC	6 cut(s)	396, 539, 733, 1318, 2593, 3036
Alw44I	GTGCAC	1 cut(s)	1624
AlwI	GGATC	6 cut(s)	609, 637, 1039, 1597, 2258, 2271
AlwNI	CAGNNNCTG	2 cut(s)	1113, 2831
Ama87I	CYCGRG	1 cut(s)	1128
Aor51HI	AGCGCT	1 cut(s)	164
AoxI	GGCC	6 cut(s)	931, 1148, 1477, 2350, 2785, 3346
ApaLI	GTGCAC	1 cut(s)	1624
ApoI	RAATTY	3 cut(s)	1820, 2246, 2802
ArsI	GACNNNNNNTTYG	2 cut(s)	1358, 1390
AseI	ATTAAT	1 cut(s)	2720
AsiGI	ACCGGT	1 cut(s)	198
Asp700I	GAANNNNTTC	3 cut(s)	72, 119, 1799
AspA2I	CCTAGG	2 cut(s)	1001, 1901
AspLEI	GCGC	6 cut(s)	165, 686, 921, 1992, 3024, 3331
AspS9I	GGNCC	7 cut(s)	127, 298, 665, 710, 858, 1125, 3347
AsuC2I	CCSGG	4 cut(s)	1129, 1130, 2428, 3403
AsuHPI	GGTGA	6 cut(s)	337, 1112, 1216, 1700, 1826, 2759
AsuII	TTCGAA	1 cut(s)	514
AvaI	CYCGRG	1 cut(s)	1128
AvaII	GGWCC	6 cut(s)	127, 298, 665, 710, 858, 1125
AvrII	CCTAGG	2 cut(s)	1001, 1901
BaeGI	GKGCMC	4 cut(s)	706, 1246, 1508, 1628
BaeI	ACNNNNGTAYC	2 cut(s)	2616, 2649
BalI	TGGCCA	1 cut(s)	933
BamHI	GGATCC	1 cut(s)	2263
BanI	GGYRCC	5 cut(s)	701, 743, 1243, 2084, 3328
BanII	GRGCYC	1 cut(s)	2049
BarI	GAAGNNNNNNTAC	2 cut(s)	808, 840
BauI	CACGAG	1 cut(s)	1593
BbrPI	CACGTG	1 cut(s)	1410
BbsI	GAAGAC	2 cut(s)	198, 503
Bbv12I	GWGCWC	2 cut(s)	909, 1628
BbvCI	CCTCAGC	1 cut(s)	2517
BceAI	ACGGC	1 cut(s)	3292
BciT130I	CCWGG	6 cut(s)	137, 741, 813, 1614, 2123, 2354
BclI	TGATCA	1 cut(s)	2953
BcnI	CCSGG	4 cut(s)	1129, 1130, 2428, 3403
BcoDI	GTCTC	6 cut(s)	396, 539, 733, 1318, 2593, 3036
BfmI	CTRYAG	2 cut(s)	864, 1446
BfoI	RGCGCY	2 cut(s)	166, 3332
BfrI	CTTAAG	2 cut(s)	302, 3145
BlnI	CCTAGG	2 cut(s)	1001, 1901
Bme18I	GGWCC	6 cut(s)	127, 298, 665, 710, 858, 1125
BmeT110I	CYCGRG	1 cut(s)	1128
BmgT120I	GGNCC	7 cut(s)	127, 298, 665, 710, 858, 1125, 3347
BmrI	ACTGGG	2 cut(s)	343, 2833
BmuI	ACTGGG	2 cut(s)	343, 2833
BpiI	GAAGAC	2 cut(s)	198, 503
BpmI	CTGGAG	2 cut(s)	162, 1335
Bpu10I	CCTNAGC	1 cut(s)	2517
Bpu14I	TTCGAA	1 cut(s)	514
BpuEI	CTTGAG	2 cut(s)	104, 2658
BpuMI	CCSGG	4 cut(s)	1129, 1130, 2428, 3403
BsaAI	YACGTR	1 cut(s)	1410
BsaHI	GRCGYC	1 cut(s)	3329
BsaI	GGTCTC	2 cut(s)	2593, 3036
BsaJI	CCNNGG	7 cut(s)	706, 854, 1001, 1128, 1901, 2121, 2207
BsaWI	WCCGGW	1 cut(s)	198
Bse118I	RCCGGY	1 cut(s)	198
Bse1I	ACTGG	8 cut(s)	349, 934, 1318, 1528, 2147, 2820, 2839, 3096
Bse3DI	GCAATG	4 cut(s)	747, 1187, 1368, 2535
BseBI	CCWGG	6 cut(s)	137, 741, 813, 1614, 2123, 2354
BseDI	CCNNGG	7 cut(s)	706, 854, 1001, 1128, 1901, 2121, 2207
BseMI	GCAATG	4 cut(s)	747, 1187, 1368, 2535
BseMII	CTCAG	8 cut(s)	1313, 1898, 2034, 2508, 3166, 3211, 3234, 3294
BseNI	ACTGG	8 cut(s)	349, 934, 1318, 1528, 2147, 2820, 2839, 3096
BseRI	GAGGAG	2 cut(s)	68, 798
BseSI	GKGCMC	4 cut(s)	706, 1246, 1508, 1628
BseYI	CCCAGC	3 cut(s)	490, 752, 3209
BsgI	GTGCAG	2 cut(s)	852, 1665
Bsh1236I	CGCG	1 cut(s)	508
BshFI	GGCC	6 cut(s)	933, 1150, 1479, 2352, 2787, 3348
BshNI	GGYRCC	5 cut(s)	701, 743, 1243, 2084, 3328
BshTI	ACCGGT	1 cut(s)	198
BsiHKAI	GWGCWC	2 cut(s)	909, 1628
BsiHKCI	CYCGRG	1 cut(s)	1128
BsiSI	CCGG	5 cut(s)	199, 1129, 1452, 2427, 3403
BslFI	GGGAC	2 cut(s)	639, 1111
BsmAI	GTCTC	6 cut(s)	396, 539, 733, 1318, 2593, 3036
BsmFI	GGGAC	2 cut(s)	639, 1111
BsmI	GAATGC	4 cut(s)	485, 921, 1271, 1373
BsnI	GGCC	6 cut(s)	933, 1150, 1479, 2352, 2787, 3348
Bso31I	GGTCTC	2 cut(s)	2593, 3036
BsoBI	CYCGRG	1 cut(s)	1128
Bsp119I	TTCGAA	1 cut(s)	514
Bsp1286I	GDGCHC	6 cut(s)	706, 909, 1246, 1508, 1628, 2049
Bsp143I	GATC	9 cut(s)	33, 342, 601, 629, 994, 1031, 1589, 2263, 2953
Bsp19I	CCATGG	1 cut(s)	706
BspACI	CCGC	7 cut(s)	1356, 1884, 2261, 2407, 2646, 3201, 3378
BspANI	GGCC	6 cut(s)	933, 1150, 1479, 2352, 2787, 3348
BspCNI	CTCAG	8 cut(s)	1314, 1899, 2035, 2509, 3165, 3210, 3235, 3293
BspFNI	CGCG	1 cut(s)	508
BspHI	TCATGA	1 cut(s)	2619
BspPI	GGATC	6 cut(s)	609, 637, 1039, 1597, 2258, 2271
BspT104I	TTCGAA	1 cut(s)	514
BspT107I	GGYRCC	5 cut(s)	701, 743, 1243, 2084, 3328
BspTI	CTTAAG	2 cut(s)	302, 3145
BspTNI	GGTCTC	2 cut(s)	2593, 3036
BsrDI	GCAATG	4 cut(s)	747, 1187, 1368, 2535
BsrFI	RCCGGY	1 cut(s)	198
BsrI	ACTGG	8 cut(s)	349, 934, 1318, 1528, 2147, 2820, 2839, 3096
BssAI	RCCGGY	1 cut(s)	198
BssECI	CCNNGG	7 cut(s)	706, 854, 1001, 1128, 1901, 2121, 2207
BssMI	GATC	9 cut(s)	33, 342, 601, 629, 994, 1031, 1589, 2263, 2953
BssNAI	GTATAC	1 cut(s)	1467
BssNI	GRCGYC	1 cut(s)	3329
BssSI	CACGAG	1 cut(s)	1593
BssT1I	CCWWGG	4 cut(s)	706, 854, 1001, 1901
Bst1107I	GTATAC	1 cut(s)	1467
Bst2BI	CACGAG	1 cut(s)	1593
Bst2UI	CCWGG	6 cut(s)	137, 741, 813, 1614, 2123, 2354
BstACI	GRCGYC	1 cut(s)	3329
BstAFI	CTTAAG	2 cut(s)	302, 3145
BstAPI	GCANNNNNTGC	4 cut(s)	2084, 2447, 3050, 3173
BstBAI	YACGTR	1 cut(s)	1410
BstBI	TTCGAA	1 cut(s)	514
BstC8I	GCNNGC	7 cut(s)	483, 682, 1893, 1988, 2189, 2533, 3008
BstDSI	CCRYGG	2 cut(s)	706, 2207
BstEII	GGTNACC	1 cut(s)	2765
BstFNI	CGCG	1 cut(s)	508
BstH2I	RGCGCY	2 cut(s)	166, 3332
BstHHI	GCGC	6 cut(s)	165, 686, 921, 1992, 3024, 3331
BstKTI	GATC	9 cut(s)	36, 345, 604, 632, 997, 1034, 1592, 2266, 2956
BstMAI	GTCTC	6 cut(s)	396, 539, 733, 1318, 2593, 3036
BstMBI	GATC	9 cut(s)	33, 342, 601, 629, 994, 1031, 1589, 2263, 2953
BstNI	CCWGG	6 cut(s)	137, 741, 813, 1614, 2123, 2354
BstNSI	RCATGY	1 cut(s)	3290
BstPI	GGTNACC	1 cut(s)	2765
BstSFI	CTRYAG	2 cut(s)	864, 1446
BstSLI	GKGCMC	4 cut(s)	706, 1246, 1508, 1628
BstUI	CGCG	1 cut(s)	508
BstV2I	GAAGAC	2 cut(s)	198, 503
BstX2I	RGATCY	3 cut(s)	629, 1031, 2263
BstXI	CCANNNNNNTGG	3 cut(s)	1861, 2460, 2659
BstYI	RGATCY	3 cut(s)	629, 1031, 2263
BstZ17I	GTATAC	1 cut(s)	1467
BsuRI	GGCC	6 cut(s)	933, 1150, 1479, 2352, 2787, 3348
BtgI	CCRYGG	2 cut(s)	706, 2207
BtsIMutI	CAGTG	6 cut(s)	927, 1230, 1506, 1928, 1960, 3288
Cac8I	GCNNGC	7 cut(s)	483, 682, 1893, 1988, 2189, 2533, 3008
CaiI	CAGNNNCTG	2 cut(s)	1113, 2831
CciI	TCATGA	1 cut(s)	2619
CfoI	GCGC	6 cut(s)	165, 686, 921, 1992, 3024, 3331
Cfr10I	RCCGGY	1 cut(s)	198
Cfr13I	GGNCC	7 cut(s)	127, 298, 665, 710, 858, 1125, 3347
Cfr9I	CCCGGG	1 cut(s)	1128
CseI	GACGC	3 cut(s)	497, 1167, 1464
Csp6I	GTAC	4 cut(s)	1017, 1142, 2813, 2998
CspAI	ACCGGT	1 cut(s)	198
CviQI	GTAC	4 cut(s)	1017, 1142, 2813, 2998
DinI	GGCGCC	1 cut(s)	3330
DpnI	GATC	9 cut(s)	35, 344, 603, 631, 996, 1033, 1591, 2265, 2955
DpnII	GATC	9 cut(s)	33, 342, 601, 629, 994, 1031, 1589, 2263, 2953
DrdI	GACNNNNNNGTC	1 cut(s)	3156
DseDI	GACNNNNNNGTC	1 cut(s)	3156
EaeI	YGGCCR	1 cut(s)	931
Eco130I	CCWWGG	4 cut(s)	706, 854, 1001, 1901
Eco147I	AGGCCT	2 cut(s)	1150, 2787
Eco24I	GRGCYC	1 cut(s)	2049
Eco31I	GGTCTC	2 cut(s)	2593, 3036
Eco32I	GATATC	1 cut(s)	1105
Eco47I	GGWCC	6 cut(s)	127, 298, 665, 710, 858, 1125
Eco47III	AGCGCT	1 cut(s)	164
Eco57I	CTGAAG	2 cut(s)	2808, 3335
Eco72I	CACGTG	1 cut(s)	1410
Eco88I	CYCGRG	1 cut(s)	1128
Eco91I	GGTNACC	1 cut(s)	2765
EcoO109I	RGGNCCY	3 cut(s)	298, 858, 1125
EcoO65I	GGTNACC	1 cut(s)	2765
EcoRII	CCWGG	6 cut(s)	135, 739, 811, 1612, 2121, 2352
EcoRV	GATATC	1 cut(s)	1105
EcoT14I	CCWWGG	4 cut(s)	706, 854, 1001, 1901
EcoT22I	ATGCAT	1 cut(s)	969
EcoT38I	GRGCYC	1 cut(s)	2049
EgeI	GGCGCC	1 cut(s)	3330
EheI	GGCGCC	1 cut(s)	3330
ErhI	CCWWGG	4 cut(s)	706, 854, 1001, 1901
FalI	AAGNNNNNCTT	2 cut(s)	981, 1013
FaqI	GGGAC	2 cut(s)	639, 1111
FauI	CCCGC	1 cut(s)	2653
FbaI	TGATCA	1 cut(s)	2953
FblI	GTMKAC	2 cut(s)	1466, 3088
FriOI	GRGCYC	1 cut(s)	2049
GlaI	GCGC	6 cut(s)	164, 685, 920, 1991, 3023, 3330
GsaI	CCCAGC	3 cut(s)	494, 756, 3213
GsuI	CTGGAG	2 cut(s)	162, 1335
HaeII	RGCGCY	2 cut(s)	166, 3332
HaeIII	GGCC	6 cut(s)	933, 1150, 1479, 2352, 2787, 3348
HapII	CCGG	5 cut(s)	199, 1129, 1452, 2427, 3403
HgaI	GACGC	3 cut(s)	497, 1167, 1464
HhaI	GCGC	6 cut(s)	165, 686, 921, 1992, 3024, 3331
Hin1I	GRCGYC	1 cut(s)	3329
Hin6I	GCGC	6 cut(s)	163, 684, 919, 1990, 3022, 3329
HinP1I	GCGC	6 cut(s)	163, 684, 919, 1990, 3022, 3329
HincII	GTYRAC	2 cut(s)	1182, 2912
HindII	GTYRAC	2 cut(s)	1182, 2912
HindIII	AAGCTT	3 cut(s)	479, 1566, 3008
HpaII	CCGG	5 cut(s)	199, 1129, 1452, 2427, 3403
HphI	GGTGA	6 cut(s)	337, 1112, 1216, 1700, 1826, 2759
Hpy166II	GTNNAC	9 cut(s)	638, 1182, 1467, 1600, 1626, 2386, 2912, 2998, 3089
Hpy8I	GTNNAC	9 cut(s)	638, 1182, 1467, 1600, 1626, 2386, 2912, 2998, 3089
HpyCH4IV	ACGT	4 cut(s)	518, 1409, 2445, 2562
HpySE526I	ACGT	4 cut(s)	518, 1409, 2445, 2562
Hsp92I	GRCGYC	1 cut(s)	3329
HspAI	GCGC	6 cut(s)	163, 684, 919, 1990, 3022, 3329
KasI	GGCGCC	1 cut(s)	3328
KflI	GGGWCCC	1 cut(s)	1125
Ksp22I	TGATCA	1 cut(s)	2953
Kzo9I	GATC	9 cut(s)	33, 342, 601, 629, 994, 1031, 1589, 2263, 2953
LmnI	GCTCC	6 cut(s)	19, 811, 1492, 1615, 2853, 3058
MaeII	ACGT	4 cut(s)	518, 1409, 2445, 2562
MaeIII	GTNAC	6 cut(s)	871, 1405, 1926, 1939, 2765, 3110
MalI	GATC	9 cut(s)	35, 344, 603, 631, 996, 1033, 1591, 2265, 2955
MboI	GATC	9 cut(s)	33, 342, 601, 629, 994, 1031, 1589, 2263, 2953
MboII	GAAGA	8 cut(s)	97, 203, 223, 503, 989, 1070, 1570, 2814
MfeI	CAATTG	2 cut(s)	538, 2694
MflI	RGATCY	3 cut(s)	629, 1031, 2263
MhlI	GDGCHC	6 cut(s)	706, 909, 1246, 1508, 1628, 2049
MlsI	TGGCCA	1 cut(s)	933
MluNI	TGGCCA	1 cut(s)	933
Mly113I	GGCGCC	1 cut(s)	3329
MlyI	GAGTC	5 cut(s)	101, 541, 1653, 2291, 3144
MmeI	TCCRAC	5 cut(s)	641, 1066, 1334, 2905, 3103
Mox20I	TGGCCA	1 cut(s)	933
Mph1103I	ATGCAT	1 cut(s)	969
MroXI	GAANNNNTTC	3 cut(s)	72, 119, 1799
MscI	TGGCCA	1 cut(s)	933
MslI	CAYNNNNRTG	2 cut(s)	1631, 2458
Msp20I	TGGCCA	1 cut(s)	933
MspA1I	CMGCKG	8 cut(s)	836, 896, 1868, 2407, 2516, 2648, 2903, 3201
MspCI	CTTAAG	2 cut(s)	302, 3145
MspI	CCGG	5 cut(s)	199, 1129, 1452, 2427, 3403
MunI	CAATTG	2 cut(s)	538, 2694
Mva1269I	GAATGC	4 cut(s)	485, 921, 1271, 1373
MvaI	CCWGG	6 cut(s)	137, 741, 813, 1614, 2123, 2354
MvnI	CGCG	1 cut(s)	508
NarI	GGCGCC	1 cut(s)	3329
NciI	CCSGG	4 cut(s)	1129, 1130, 2428, 3403
NcoI	CCATGG	1 cut(s)	706
NdeII	GATC	9 cut(s)	33, 342, 601, 629, 994, 1031, 1589, 2263, 2953
NmuCI	GTSAC	4 cut(s)	1405, 1926, 2765, 3110
NsiI	ATGCAT	1 cut(s)	969
NspI	RCATGY	1 cut(s)	3290
NspV	TTCGAA	1 cut(s)	514
OliI	CACNNNNGTG	1 cut(s)	2458
PagI	TCATGA	1 cut(s)	2619
PceI	AGGCCT	2 cut(s)	1150, 2787
PctI	GAATGC	4 cut(s)	485, 921, 1271, 1373
PdmI	GAANNNNTTC	3 cut(s)	72, 119, 1799
PflFI	GACNNNGTC	1 cut(s)	131
PflMI	CCANNNNNTGG	4 cut(s)	680, 740, 1702, 2474
PfoI	TCCNGGA	2 cut(s)	1612, 2426
PinAI	ACCGGT	1 cut(s)	198
PleI	GAGTC	5 cut(s)	100, 540, 1653, 2291, 3144
PluTI	GGCGCC	1 cut(s)	3332
PmaCI	CACGTG	1 cut(s)	1410
PmlI	CACGTG	1 cut(s)	1410
PpsI	GAGTC	5 cut(s)	100, 540, 1653, 2291, 3144
Ppu21I	YACGTR	1 cut(s)	1410
PpuMI	RGGWCCY	3 cut(s)	298, 858, 1125
PshBI	ATTAAT	1 cut(s)	2720
PsiI	TTATAA	1 cut(s)	2727
Psp1406I	AACGTT	1 cut(s)	518
Psp5II	RGGWCCY	3 cut(s)	298, 858, 1125
Psp6I	CCWGG	6 cut(s)	135, 739, 811, 1612, 2121, 2352
PspCI	CACGTG	1 cut(s)	1410
PspEI	GGTNACC	1 cut(s)	2765
PspFI	CCCAGC	3 cut(s)	490, 752, 3209
PspGI	CCWGG	6 cut(s)	135, 739, 811, 1612, 2121, 2352
PspPI	GGNCC	7 cut(s)	127, 298, 665, 710, 858, 1125, 3347
PspPPI	RGGWCCY	3 cut(s)	298, 858, 1125
PstNI	CAGNNNCTG	2 cut(s)	1113, 2831
PsuI	RGATCY	3 cut(s)	629, 1031, 2263
PsyI	GACNNNGTC	1 cut(s)	131
PvuII	CAGCTG	5 cut(s)	836, 896, 1868, 2516, 2903
RsaI	GTAC	4 cut(s)	1018, 1143, 2814, 2999
RsaNI	GTAC	4 cut(s)	1017, 1142, 2813, 2998
RseI	CAYNNNNRTG	2 cut(s)	1631, 2458
Sau3AI	GATC	9 cut(s)	33, 342, 601, 629, 994, 1031, 1589, 2263, 2953
Sau96I	GGNCC	7 cut(s)	127, 298, 665, 710, 858, 1125, 3347
SchI	GAGTC	5 cut(s)	101, 541, 1653, 2291, 3144
SduI	GDGCHC	6 cut(s)	706, 909, 1246, 1508, 1628, 2049
SfcI	CTRYAG	2 cut(s)	864, 1446
SfoI	GGCGCC	1 cut(s)	3330
SfuI	TTCGAA	1 cut(s)	514
SinI	GGWCC	6 cut(s)	127, 298, 665, 710, 858, 1125
SmaI	CCCGGG	1 cut(s)	1130
SmiMI	CAYNNNNRTG	2 cut(s)	1631, 2458
SmlI	CTYRAG	4 cut(s)	83, 302, 2673, 3145
SmoI	CTYRAG	4 cut(s)	83, 302, 2673, 3145
SseBI	AGGCCT	2 cut(s)	1150, 2787
SsiI	CCGC	7 cut(s)	1356, 1884, 2261, 2407, 2646, 3201, 3378
SspDI	GGCGCC	1 cut(s)	3328
StuI	AGGCCT	2 cut(s)	1150, 2787
StyI	CCWWGG	4 cut(s)	706, 854, 1001, 1901
TaiI	ACGT	4 cut(s)	521, 1412, 2448, 2565
TaqI	TCGA	5 cut(s)	123, 418, 514, 2032, 3160
TatI	WGTACW	1 cut(s)	2812
TscAI	CASTG	6 cut(s)	934, 1230, 1506, 1935, 1960, 3288
TseFI	GTSAC	4 cut(s)	1405, 1926, 2765, 3110
Tsp45I	GTSAC	4 cut(s)	1405, 1926, 2765, 3110
TspGWI	ACGGA	4 cut(s)	642, 1004, 1750, 2224
TspMI	CCCGGG	1 cut(s)	1128
TspRI	CASTG	6 cut(s)	934, 1230, 1506, 1935, 1960, 3288
Tth111I	GACNNNGTC	1 cut(s)	131
Van91I	CCANNNNNTGG	4 cut(s)	680, 740, 1702, 2474
Vha464I	CTTAAG	2 cut(s)	302, 3145
VneI	GTGCAC	1 cut(s)	1624
VpaK11BI	GGWCC	6 cut(s)	127, 298, 665, 710, 858, 1125
VspI	ATTAAT	1 cut(s)	2720
XapI	RAATTY	3 cut(s)	1820, 2246, 2802
XbaI	TCTAGA	1 cut(s)	3337
XceI	RCATGY	1 cut(s)	3290
XcmI	CCANNNNNNNNNTGG	3 cut(s)	137, 2350, 3217
XmaI	CCCGGG	1 cut(s)	1128
XmaJI	CCTAGG	2 cut(s)	1001, 1901
XmiI	GTMKAC	2 cut(s)	1466, 3088
XmnI	GAANNNNTTC	3 cut(s)	72, 119, 1799
Zsp2I	ATGCAT	1 cut(s)	969

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.