Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0002550

Physical Location & Seq

Genomic Coordinates Forward (+)

36288537 .. 36296565

Gene Structure 8029 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1306300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3402 bp

ATGTCTTCGTTAAGCAGGGAGCTGGTATTCCTGATCTTACAGTTCTTAGATGAGGAGAAGTTCAAGGAAACAGTTCATAAGCTTGAGCAAGAGTCTGGTTTTTTCTTCAATATGAAGCATTTTGAGGACCAAGTCCAGGCTGGAGAGTGGGATGAAGTTGAGCGCTATTTGTGTGGCTTCACTAAAGTTGAAGACAACCGGTATTCAATGAAGATTTTTTTTGAAATTAGGAAGCAGAAGTATTTGGAAGCTCTTGACAGGCAGGATAGGGCAAGGGCTGTTGAGATTCTTGTGAAGGACCTTAAGGTTTTTTCATCATTTAATGAGGAACTTTTCAAGGAGATCACCCAGTTGCTTACTCTTGATAACTTTAGGCAAAATGAGCAGCTTTCCAAGTACGGAGACACAAAATCGGCTCGGAATATCATGCTTATAGAACTTAAAAAGCTCATTGAGGCGAACCCATTATTTCGTGATAAGCTTGCATTCCCAGCCTTCAAAAGTTCACGACTCCGAACGTTAATCAACCAGAGTCTCAATTGGCAGCACCAGCTTTGCAAGAATCCCCGTCCAAACCCTGATATTAAAACACTATTTATGGATCATTCTTGCACTCCAACTGCTAACGGATCTCGTCCTCCACCCACAAATAGTCCCCTTGTAGGACCAATTCCAAAGGCTGGCGCCTTTCCTCCTATTGGTGCCCTTGGTCCATTTCAACCTGTTGTCTCCCCATCTCCTGGTGCCATTGCTGGGTGGATGTCAAGCACTAACCCTCCTTTGCCCCACCCTGGTGTTGCAGCAGCTCCTCCTGGTATGCAGCCTTCTAGTGCAGCTGCTTTCCTGAAGCACCCAAGGACCCCTACAGGTGTTACAGGGATGGATTATCAATCAGCTGATTCAGAGCACTTGATGAAGCGTATTCGCACTGGTCAAGGTGACGAGGTGTCCTTTTCTGGTGTGATGCATAATTCCAATGTCTATTCACAAGATGACCTTCCCAAGGCTGTTGTTCGTACCCTTAGTCAAGGATCTAATGTGATGAGCATGGACTTCCATCCACAACAACAAACTATTCTTCTAGTTGGAACAAATGTTGGTGATATCAGCCTCTGGGAAGTAGGGTCCCGGGAGAGGTTGGTACATAAGCCTTTCAAGGTTTGGGATATACAAACCGCGTCAACACCGTTGCAGACAGCTCTGGTGAATGATGCTGCAATATCAGTTAATCGTTGTGTATGGGCGCCAGATGGGTTTATGTTGGGTGTTGCATTCTCTAAGCACATTGTTCAGATATATAATTACAATCCAACTGGAGAACTGAGACAACATTTGGAGATTGATGCTCATGTTGGTGGTGTTAATGACATTGCATTCGCTCATCCCAACAAGCAATTGTGTATTGTTACGTGTGGAGATGATAGAGTGATTAAGGTATGGGATGCTGTAGCCGGACGCAAACAGTATACATTTGAAGGCCATGAAGCTCCTGTGTATTCAGTATGCCCTCATTACAAAGAAAATATCCAGTTCATTTTTTCAACTGCCATTGACGGGAAGATTAAAGCTTGGCTATATGATTGCTTGGGATCACGAGTGGATTATGATGCGCCTGGACTTTGGTGCACTATGATGTCATATAGTGCAGATGGAACCAGACTCTTCTCATGCGGAACAAGTAAAGAAGGTGAATCCCATCTGGTGGAGTGGAATGAGAGTGAAGGGGCTATCAAAAGGACATATTCTGGTTTTAGAAAACGCTCTTTAGATGTTGTTCAGTTTGACACAACAAGGAACCGATTCTTAGCTGCTGGTGATGAATTTCAAATTAAGTTCTGGGATATGGACAATACCAATGTGTTGACAGCTGTGGATGCAGATGGCGGTTTGCCTCCTAGTCCTAGACTGAGATTCAATAAAGAAGGGTCACTGTTGGCTGTAGCTACTAATGACAGTGGTATTAAGATTTTAGCAAATAATGATGGCTTGCGCTTGATTAGAATGTTGGAGGGAAGGGCTATGGAGAAAAATAGAGGCACTACTGAGCCCATCAACTCTAAGCCTCTGATGGTCAATGCCATAGGCGCCATTGTGAATGTTCCTAACGCTGTTGCTCCAGCCCTGGAACGTCCTGATAGAATACAACCAACTGTGTCTATCAGTAATCTAGGTACTATGGAGAACAGCAGGCTTGTTGATGTTAAACCACGGATTTCTGATGATATAGACAAGATTAAGAGCTGGAAAATTTCTGATGTTGCGGATCCCTCTCAAATGAAAGCTCTCCGACTTCCTGACTCTACAATTGCTGGAAAGATTGTGAGACTAATGTACACCAACAATGGCCTGGCTTTGTTAGCCCTTGCCTCCAATGCTGTTCACAAGCTTTGGAAATGGCAGCGTAATGAAAGGAATCCATCCGGGAAGGCTACTGCATATGTTGCACCACAGTTGTGGCAGCCTCCCAATGGAACTCTTATGACTAACGATGTAAATGACAATAAACCAGCTGAGGAGTCTACTGCATGCATTGCTTTATCCAAGAATGATTCTTACGTCATGTCTGCATCTGGTGGGAAGGTCTCTTTGTTCAACATGATGACATTTAAGGTCATGACAACATTTGTATCTCCCCCTCCCGCAGCCACTTTTTTGGCATTTCATCCTCAAGACAATAATATAATTGCAATTGGCATGGAGGATTCTACTATATTAATATATAATGTTAGGGTTGATGAGGTCAAAACCAAACTGAAAGGTCACCAGAATCGGATTACTGGCCTTGCATTTTCCCAAATCCATAATGTACTGGTATCTTCAGGTGCTGATACCCAGTTATGTGTGTGGAGCATTGATGGATGGGAGAAAAAGAAAACAAGGTTCATACAAGCACCAGCTGGTCGCCAACCCCCGATGGTTGGAGAAACAAAAGTTCAGTTTCATAATGATCACACACACCTGTTGGTTGCCCATGAAAGCCAAGTTGCTGTGTACGATTGCAAGCTTGAATGTTTGTGCTCGTGGTCTCCGAAAGATGCACTCACTGCTCCCATATCAAGTGCAATATATTCATGCGATGGTCTACTGGTTTATGCTTCTTTTTGTGACGGTGCTGTTGGAGTTTTTGATGCTGATACCTTAAGACTCAGATGTCGAATAGCACCTACTGCTTATATACCATCATTTTCTCTCAGCGGAAACCCCAGCTATGCTTTGGTTATAGCAGCTCATCCTTCTGAGCCTAATCAGATTGCACTTGGCATGACTGACGGCTCAGTGCATGTAGTTGAACCATCTGATGCAGAGCTGAAGTGGGGTGGGGCGCCCCCTCAAGACAACGGCCCTTCCAATTCGTCAAATCCTTCTCCTAGCGGCCAAGCATCTGAGCATCCTTCCCGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003002 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009753 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009867 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009933 GO:0009987 GO:0010014 GO:0010016 GO:0010033 GO:0010051 GO:0010072 GO:0010154 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032870 GO:0042221 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045892 GO:0045934 GO:0046983 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048519 GO:0048523 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0080090 GO:0090421 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

1134

Amino Acids

125.2

Weight (kDa)

6.65

Isoelectric Point (pI)

39.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LisH_TPL	PF17814	5 - 34	6.8e-13	LisH-like dimerisation domain
TPR1-like_2nd	PF21889	37 - 161	1.4e-61	TPR1-like, CTLH-containing domain
zf_topless	PF21359	164 - 204	1.6e-25	TOPLESS, zinc finger domain
WD40_CDC20-Fz	PF24807	325 - 613	3.1e-08	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd	PF25172	345 - 561	3.1e-07	WDR3 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	386 - 558	6.8e-14	WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	386 - 657	1.6e-07	WDR3 second beta-propeller domain
WD40_Gbeta	PF25391	406 - 524	7.2e-07	G protein beta WD-40 repeat protein
Beta-prop_THOC3	PF25174	408 - 666	1.4e-20	THOC3 beta-propeller domain
Beta-prop_WDR5	PF25175	409 - 621	5.9e-19	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	421 - 525	1.3e-09	WDHD1 first WD40 domain
WD40_Prp19	PF24814	451 - 656	1.3e-10	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	566 - 672	1.4e-06	WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz	PF24807	831 - 956	1.2e-13	CDC20/Fizzy WD40 domain
WD40_WDHD1_1st	PF24817	835 - 1048	1.3e-19	WDHD1 first WD40 domain
Beta-prop_WDR3_1st	PF25173	835 - 953	3.1e-17	WDR3 first beta-propeller domain
Beta-prop_EML_2	PF23414	836 - 957	7.9e-11	Echinoderm microtubule-associated protein second beta-propeller
WDR55	PF24796	837 - 951	1.6e-06	WDR55
Beta-prop_WDR5	PF25175	838 - 1093	6.9e-16	WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	841 - 952	1.1e-09	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	843 - 959	3.7e-12	WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	854 - 953	8e-11	WDR36/Utp21 first beta-propeller
Beta-prop_IFT140_1st	PF23383	854 - 954	2.5e-11	IFT140 first beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	860 - 957	2e-08	WDR90/POC16, second beta-propeller
Beta-prop_RIG_1st	PF23770	868 - 936	7.7e-08	RIG first beta-propeller
WD40_Prp19	PF24814	879 - 1073	7.1e-15	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	901 - 1095	6.2e-09	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002550)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770
malus_domestica	MD01G1234100.v1.1 MD07G1306300.v1.1
prunus_persica	Prupe.2G324300_v2.0.a1
pyrus_communis	pycom01g24260 pycom07g27930
rosa_chinensis	RchiOBHm_Chr1g0382471 RchiOBHm_Chr1g0382481 RchiOBHm_Chr1g0383911
rosa_laevigata	RLG00000026087 RLG00000026174 RLG00000026177
rosa_multiflora	Rmu_sc0000110.1_g000051 Rmu_sc0003885.1_g000009 Rmu_sc0005139.1_g000028
rosa_roxburghii	Rroxscaffold_4G00277160 Rroxscaffold_4G00278020 Rroxscaffold_4G00278080
rosa_rugosa	Rorug01G0434600 Rorug01G0434900 Rorug01G0443000
rosa_samantha	Rh1AG459200 Rh1AG459300 Rh1AG459600 Rh1AG468900 Rh1BG415700 Rh1BG426600 Rh1CG429700 Rh1CG429800 Rh1CG439400 Rh1DG447800 Rh1DG447900 Rh1DG459400
rosa_wichuraiana	Rw1G040350 Rw1G041180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	3150
AccB1I	GGYRCC	6 cut(s)	683, 701, 743, 1243, 2084, 3322
AccB7I	CCANNNNNTGG	3 cut(s)	680, 740, 1702
AccI	GTMKAC	3 cut(s)	1466, 2519, 3082
AccII	CGCG	1 cut(s)	1178
AciI	CCGC	7 cut(s)	1176, 1671, 1884, 2261, 2640, 3195, 3372
AclI	AACGTT	1 cut(s)	518
AclWI	GGATC	6 cut(s)	609, 637, 1039, 1597, 2258, 2271
AcoI	YGGCCR	1 cut(s)	3373
AcsI	RAATTY	2 cut(s)	1820, 2247
AcuI	CTGAAG	3 cut(s)	866, 2802, 3329
AcyI	GRCGYC	4 cut(s)	684, 1244, 2085, 3323
AfaI	GTAC	7 cut(s)	398, 1018, 1143, 2173, 2333, 2808, 2993
AfeI	AGCGCT	1 cut(s)	164
AflII	CTTAAG	2 cut(s)	302, 3139
AflIII	ACRYGT	1 cut(s)	1409
AgeI	ACCGGT	1 cut(s)	198
AhdI	GACNNNNNGTC	1 cut(s)	946
AjnI	CCWGG	7 cut(s)	135, 739, 790, 811, 1612, 2121, 2346
AleI	CACNNNNGTG	2 cut(s)	792, 2452
Alw21I	GWGCWC	3 cut(s)	909, 1628, 3020
Alw26I	GTCTC	7 cut(s)	396, 539, 733, 1318, 2317, 2587, 3030
Alw44I	GTGCAC	1 cut(s)	1624
AlwI	GGATC	6 cut(s)	609, 637, 1039, 1597, 2258, 2271
AlwNI	CAGNNNCTG	2 cut(s)	1113, 2825
Ama87I	CYCGRG	1 cut(s)	1128
Aor51HI	AGCGCT	1 cut(s)	164
AoxI	GGCC	5 cut(s)	1477, 2344, 2779, 3340, 3373
ApaLI	GTGCAC	1 cut(s)	1624
ApoI	RAATTY	2 cut(s)	1820, 2247
ArsI	GACNNNNNNTTYG	2 cut(s)	1358, 1390
AseI	ATTAAT	1 cut(s)	2714
AsiGI	ACCGGT	1 cut(s)	198
Asp700I	GAANNNNTTC	2 cut(s)	72, 1799
AspLEI	GCGC	7 cut(s)	165, 686, 1246, 1612, 1992, 2087, 3325
AspS9I	GGNCC	7 cut(s)	127, 298, 665, 710, 858, 1125, 3341
AsuC2I	CCSGG	4 cut(s)	1129, 1130, 2422, 3397
AsuHPI	GGTGA	7 cut(s)	337, 950, 1112, 1216, 1700, 1826, 2753
AvaI	CYCGRG	1 cut(s)	1128
AvaII	GGWCC	6 cut(s)	127, 298, 665, 710, 858, 1125
BaeGI	GKGCMC	2 cut(s)	706, 1628
BaeI	ACNNNNGTAYC	2 cut(s)	2610, 2643
BamHI	GGATCC	1 cut(s)	2263
BanI	GGYRCC	6 cut(s)	683, 701, 743, 1243, 2084, 3322
BanII	GRGCYC	1 cut(s)	2049
BarI	GAAGNNNNNNTAC	2 cut(s)	808, 840
BauI	CACGAG	2 cut(s)	1593, 3019
BbsI	GAAGAC	1 cut(s)	198
Bbv12I	GWGCWC	3 cut(s)	909, 1628, 3020
BbvCI	CCTCAGC	1 cut(s)	2511
BceAI	ACGGC	2 cut(s)	3286, 3355
BciT130I	CCWGG	7 cut(s)	137, 741, 792, 813, 1614, 2123, 2348
BclI	TGATCA	1 cut(s)	2947
BcnI	CCSGG	4 cut(s)	1129, 1130, 2422, 3397
BcoDI	GTCTC	7 cut(s)	396, 539, 733, 1318, 2317, 2587, 3030
BfaI	CTAG	6 cut(s)	828, 1082, 1896, 1902, 2168, 3369
BfmI	CTRYAG	3 cut(s)	864, 1446, 1938
BfoI	RGCGCY	5 cut(s)	166, 687, 1247, 2088, 3326
BfrI	CTTAAG	2 cut(s)	302, 3139
Bme18I	GGWCC	6 cut(s)	127, 298, 665, 710, 858, 1125
BmeRI	GACNNNNNGTC	1 cut(s)	946
BmeT110I	CYCGRG	1 cut(s)	1128
BmgT120I	GGNCC	7 cut(s)	127, 298, 665, 710, 858, 1125, 3341
BmrI	ACTGGG	2 cut(s)	343, 2827
BmuI	ACTGGG	2 cut(s)	343, 2827
BpiI	GAAGAC	1 cut(s)	198
BpmI	CTGGAG	3 cut(s)	162, 1335, 2100
Bpu10I	CCTNAGC	1 cut(s)	2511
BpuEI	CTTGAG	3 cut(s)	104, 2652, 3315
BpuMI	CCSGG	4 cut(s)	1129, 1130, 2422, 3397
BsaAI	YACGTR	1 cut(s)	1410
BsaHI	GRCGYC	4 cut(s)	684, 1244, 2085, 3323
BsaI	GGTCTC	2 cut(s)	2587, 3030
BsaJI	CCNNGG	7 cut(s)	706, 790, 854, 1002, 1128, 2121, 2207
BsaWI	WCCGGW	1 cut(s)	198
BsaXI	ACNNNNNCTCC	2 cut(s)	760, 790
Bse118I	RCCGGY	1 cut(s)	198
Bse1I	ACTGG	8 cut(s)	349, 934, 1318, 1528, 2782, 2814, 2833, 3090
Bse3DI	GCAATG	3 cut(s)	747, 1368, 2529
BseBI	CCWGG	7 cut(s)	137, 741, 792, 813, 1614, 2123, 2348
BseDI	CCNNGG	7 cut(s)	706, 790, 854, 1002, 1128, 2121, 2207
BseMI	GCAATG	3 cut(s)	747, 1368, 2529
BseMII	CTCAG	9 cut(s)	1313, 1898, 2034, 2502, 3160, 3205, 3228, 3288, 3375
BseNI	ACTGG	8 cut(s)	349, 934, 1318, 1528, 2782, 2814, 2833, 3090
BseRI	GAGGAG	3 cut(s)	68, 798, 2528
BseSI	GKGCMC	2 cut(s)	706, 1628
BseYI	CCCAGC	3 cut(s)	490, 752, 3203
BsgI	GTGCAG	2 cut(s)	852, 1665
Bsh1236I	CGCG	1 cut(s)	1178
BshFI	GGCC	5 cut(s)	1479, 2346, 2781, 3342, 3375
BshNI	GGYRCC	6 cut(s)	683, 701, 743, 1243, 2084, 3322
BshTI	ACCGGT	1 cut(s)	198
BsiHKAI	GWGCWC	3 cut(s)	909, 1628, 3020
BsiHKCI	CYCGRG	1 cut(s)	1128
BsiSI	CCGG	5 cut(s)	199, 1129, 1452, 2421, 3397
BslFI	GGGAC	2 cut(s)	639, 1111
BsmAI	GTCTC	7 cut(s)	396, 539, 733, 1318, 2317, 2587, 3030
BsmFI	GGGAC	2 cut(s)	639, 1111
BsmI	GAATGC	3 cut(s)	485, 1271, 1373
BsnI	GGCC	5 cut(s)	1479, 2346, 2781, 3342, 3375
Bso31I	GGTCTC	2 cut(s)	2587, 3030
BsoBI	CYCGRG	1 cut(s)	1128
Bsp1286I	GDGCHC	5 cut(s)	706, 909, 1628, 2049, 3020
Bsp1407I	TGTACA	1 cut(s)	2331
Bsp143I	GATC	8 cut(s)	33, 342, 601, 629, 1031, 1589, 2263, 2947
BspACI	CCGC	7 cut(s)	1176, 1671, 1884, 2261, 2640, 3195, 3372
BspANI	GGCC	5 cut(s)	1479, 2346, 2781, 3342, 3375
BspCNI	CTCAG	9 cut(s)	1314, 1899, 2035, 2503, 3159, 3204, 3229, 3287, 3376
BspFNI	CGCG	1 cut(s)	1178
BspHI	TCATGA	1 cut(s)	2613
BspPI	GGATC	6 cut(s)	609, 637, 1039, 1597, 2258, 2271
BspT107I	GGYRCC	6 cut(s)	683, 701, 743, 1243, 2084, 3322
BspTI	CTTAAG	2 cut(s)	302, 3139
BspTNI	GGTCTC	2 cut(s)	2587, 3030
BsrDI	GCAATG	3 cut(s)	747, 1368, 2529
BsrFI	RCCGGY	1 cut(s)	198
BsrGI	TGTACA	1 cut(s)	2331
BsrI	ACTGG	8 cut(s)	349, 934, 1318, 1528, 2782, 2814, 2833, 3090
BssAI	RCCGGY	1 cut(s)	198
BssECI	CCNNGG	7 cut(s)	706, 790, 854, 1002, 1128, 2121, 2207
BssMI	GATC	8 cut(s)	33, 342, 601, 629, 1031, 1589, 2263, 2947
BssNAI	GTATAC	1 cut(s)	1467
BssNI	GRCGYC	4 cut(s)	684, 1244, 2085, 3323
BssSI	CACGAG	2 cut(s)	1593, 3019
BssT1I	CCWWGG	3 cut(s)	706, 854, 1002
Bst1107I	GTATAC	1 cut(s)	1467
Bst2BI	CACGAG	2 cut(s)	1593, 3019
Bst2UI	CCWGG	7 cut(s)	137, 741, 792, 813, 1614, 2123, 2348
Bst4CI	ACNGT	9 cut(s)	42, 73, 1188, 1464, 1932, 1955, 2152, 2451, 3110
Bst6I	CTCTTC	1 cut(s)	1667
BstACI	GRCGYC	4 cut(s)	684, 1244, 2085, 3323
BstAFI	CTTAAG	2 cut(s)	302, 3139
BstAPI	GCANNNNNTGC	4 cut(s)	2441, 2531, 3044, 3167
BstAUI	TGTACA	1 cut(s)	2331
BstBAI	YACGTR	1 cut(s)	1410
BstC8I	GCNNGC	6 cut(s)	483, 682, 1988, 2189, 2527, 3002
BstDSI	CCRYGG	1 cut(s)	2207
BstEII	GGTNACC	1 cut(s)	2759
BstENI	CCTNNNNNAGG	1 cut(s)	1001
BstFNI	CGCG	1 cut(s)	1178
BstH2I	RGCGCY	5 cut(s)	166, 687, 1247, 2088, 3326
BstHHI	GCGC	7 cut(s)	165, 686, 1246, 1612, 1992, 2087, 3325
BstKTI	GATC	8 cut(s)	36, 345, 604, 632, 1034, 1592, 2266, 2950
BstMAI	GTCTC	7 cut(s)	396, 539, 733, 1318, 2317, 2587, 3030
BstMBI	GATC	8 cut(s)	33, 342, 601, 629, 1031, 1589, 2263, 2947
BstNI	CCWGG	7 cut(s)	137, 741, 792, 813, 1614, 2123, 2348
BstNSI	RCATGY	2 cut(s)	2529, 3284
BstPI	GGTNACC	1 cut(s)	2759
BstSFI	CTRYAG	3 cut(s)	864, 1446, 1938
BstSLI	GKGCMC	2 cut(s)	706, 1628
BstUI	CGCG	1 cut(s)	1178
BstV2I	GAAGAC	1 cut(s)	198
BstX2I	RGATCY	3 cut(s)	629, 1031, 2263
BstXI	CCANNNNNNTGG	2 cut(s)	2454, 2653
BstYI	RGATCY	3 cut(s)	629, 1031, 2263
BstZ17I	GTATAC	1 cut(s)	1467
BsuRI	GGCC	5 cut(s)	1479, 2346, 2781, 3342, 3375
BtgI	CCRYGG	1 cut(s)	2207
BtgZI	GCGATG	1 cut(s)	3090
BtsI	GCAGTG	1 cut(s)	3042
BtsIMutI	CAGTG	5 cut(s)	927, 1928, 1960, 3042, 3282
Cac8I	GCNNGC	6 cut(s)	483, 682, 1988, 2189, 2527, 3002
CaiI	CAGNNNCTG	2 cut(s)	1113, 2825
CciI	TCATGA	1 cut(s)	2613
CfoI	GCGC	7 cut(s)	165, 686, 1246, 1612, 1992, 2087, 3325
Cfr10I	RCCGGY	1 cut(s)	198
Cfr13I	GGNCC	7 cut(s)	127, 298, 665, 710, 858, 1125, 3341
Cfr9I	CCCGGG	1 cut(s)	1128
CseI	GACGC	2 cut(s)	1167, 1464
Csp6I	GTAC	7 cut(s)	397, 1017, 1142, 2172, 2332, 2807, 2992
CspAI	ACCGGT	1 cut(s)	198
CviQI	GTAC	7 cut(s)	397, 1017, 1142, 2172, 2332, 2807, 2992
DinI	GGCGCC	4 cut(s)	685, 1245, 2086, 3324
DpnI	GATC	8 cut(s)	35, 344, 603, 631, 1033, 1591, 2265, 2949
DpnII	GATC	8 cut(s)	33, 342, 601, 629, 1031, 1589, 2263, 2947
DrdI	GACNNNNNNGTC	1 cut(s)	3150
DriI	GACNNNNNGTC	1 cut(s)	946
DseDI	GACNNNNNNGTC	1 cut(s)	3150
EaeI	YGGCCR	1 cut(s)	3373
Eam1104I	CTCTTC	1 cut(s)	1667
Eam1105I	GACNNNNNGTC	1 cut(s)	946
EarI	CTCTTC	1 cut(s)	1667
Eco130I	CCWWGG	3 cut(s)	706, 854, 1002
Eco24I	GRGCYC	1 cut(s)	2049
Eco31I	GGTCTC	2 cut(s)	2587, 3030
Eco32I	GATATC	1 cut(s)	1105
Eco47I	GGWCC	6 cut(s)	127, 298, 665, 710, 858, 1125
Eco47III	AGCGCT	1 cut(s)	164
Eco57I	CTGAAG	3 cut(s)	866, 2802, 3329
Eco88I	CYCGRG	1 cut(s)	1128
Eco91I	GGTNACC	1 cut(s)	2759
EcoNI	CCTNNNNNAGG	1 cut(s)	1001
EcoO109I	RGGNCCY	3 cut(s)	298, 858, 1125
EcoO65I	GGTNACC	1 cut(s)	2759
EcoRII	CCWGG	7 cut(s)	135, 739, 790, 811, 1612, 2121, 2346
EcoRV	GATATC	1 cut(s)	1105
EcoT14I	CCWWGG	3 cut(s)	706, 854, 1002
EcoT22I	ATGCAT	2 cut(s)	969, 2531
EcoT38I	GRGCYC	1 cut(s)	2049
EgeI	GGCGCC	4 cut(s)	685, 1245, 2086, 3324
EheI	GGCGCC	4 cut(s)	685, 1245, 2086, 3324
ErhI	CCWWGG	3 cut(s)	706, 854, 1002
FalI	AAGNNNNNCTT	2 cut(s)	981, 1013
FaqI	GGGAC	2 cut(s)	639, 1111
FauI	CCCGC	1 cut(s)	2647
FauNDI	CATATG	1 cut(s)	2437
FbaI	TGATCA	1 cut(s)	2947
FblI	GTMKAC	3 cut(s)	1466, 2519, 3082
FriOI	GRGCYC	1 cut(s)	2049
FspBI	CTAG	6 cut(s)	828, 1082, 1896, 1902, 2168, 3369
GlaI	GCGC	7 cut(s)	164, 685, 1245, 1611, 1991, 2086, 3324
GsaI	CCCAGC	3 cut(s)	494, 756, 3207
GsuI	CTGGAG	3 cut(s)	162, 1335, 2100
HaeII	RGCGCY	5 cut(s)	166, 687, 1247, 2088, 3326
HaeIII	GGCC	5 cut(s)	1479, 2346, 2781, 3342, 3375
HapII	CCGG	5 cut(s)	199, 1129, 1452, 2421, 3397
HgaI	GACGC	2 cut(s)	1167, 1464
HhaI	GCGC	7 cut(s)	165, 686, 1246, 1612, 1992, 2087, 3325
Hin1I	GRCGYC	4 cut(s)	684, 1244, 2085, 3323
Hin6I	GCGC	7 cut(s)	163, 684, 1244, 1610, 1990, 2085, 3323
HinP1I	GCGC	7 cut(s)	163, 684, 1244, 1610, 1990, 2085, 3323
HincII	GTYRAC	2 cut(s)	1182, 1863
HindII	GTYRAC	2 cut(s)	1182, 1863
HindIII	AAGCTT	5 cut(s)	80, 479, 1566, 2384, 3002
HpaII	CCGG	5 cut(s)	199, 1129, 1452, 2421, 3397
HphI	GGTGA	7 cut(s)	337, 950, 1112, 1216, 1700, 1826, 2753
HpyCH4III	ACNGT	9 cut(s)	42, 73, 1188, 1464, 1932, 1955, 2152, 2451, 3110
HpyCH4IV	ACGT	4 cut(s)	518, 1409, 2128, 2556
HpySE526I	ACGT	4 cut(s)	518, 1409, 2128, 2556
Hsp92I	GRCGYC	4 cut(s)	684, 1244, 2085, 3323
HspAI	GCGC	7 cut(s)	163, 684, 1244, 1610, 1990, 2085, 3323
KasI	GGCGCC	4 cut(s)	683, 1243, 2084, 3322
KflI	GGGWCCC	1 cut(s)	1125
Ksp22I	TGATCA	1 cut(s)	2947
Kzo9I	GATC	8 cut(s)	33, 342, 601, 629, 1031, 1589, 2263, 2947
LmnI	GCTCC	6 cut(s)	19, 811, 1492, 2119, 2847, 3052
MaeI	CTAG	6 cut(s)	828, 1082, 1896, 1902, 2168, 3369
MaeII	ACGT	4 cut(s)	518, 1409, 2128, 2556
MaeIII	GTNAC	6 cut(s)	871, 938, 1405, 1926, 2759, 3104
MalI	GATC	8 cut(s)	35, 344, 603, 631, 1033, 1591, 2265, 2949
MboI	GATC	8 cut(s)	33, 342, 601, 629, 1031, 1589, 2263, 2947
MboII	GAAGA	7 cut(s)	97, 203, 223, 1070, 1570, 1654, 2808
MfeI	CAATTG	4 cut(s)	538, 1394, 2304, 2688
MflI	RGATCY	3 cut(s)	629, 1031, 2263
MhlI	GDGCHC	5 cut(s)	706, 909, 1628, 2049, 3020
Mly113I	GGCGCC	4 cut(s)	684, 1244, 2085, 3323
MlyI	GAGTC	7 cut(s)	101, 504, 541, 1653, 2291, 2525, 3138
MmeI	TCCRAC	7 cut(s)	641, 1066, 1334, 1986, 2311, 2899, 3097
Mph1103I	ATGCAT	2 cut(s)	969, 2531
MroXI	GAANNNNTTC	2 cut(s)	72, 1799
MslI	CAYNNNNRTG	4 cut(s)	792, 1353, 1631, 2452
MspA1I	CMGCKG	6 cut(s)	836, 896, 1868, 2510, 2897, 3195
MspCI	CTTAAG	2 cut(s)	302, 3139
MspI	CCGG	5 cut(s)	199, 1129, 1452, 2421, 3397
MunI	CAATTG	4 cut(s)	538, 1394, 2304, 2688
Mva1269I	GAATGC	3 cut(s)	485, 1271, 1373
MvaI	CCWGG	7 cut(s)	137, 741, 792, 813, 1614, 2123, 2348
MvnI	CGCG	1 cut(s)	1178
NarI	GGCGCC	4 cut(s)	684, 1244, 2085, 3323
NciI	CCSGG	4 cut(s)	1129, 1130, 2422, 3397
NdeI	CATATG	1 cut(s)	2437
NdeII	GATC	8 cut(s)	33, 342, 601, 629, 1031, 1589, 2263, 2947
NmuCI	GTSAC	4 cut(s)	938, 1926, 2759, 3104
NsiI	ATGCAT	2 cut(s)	969, 2531
NspI	RCATGY	2 cut(s)	2529, 3284
OliI	CACNNNNGTG	2 cut(s)	792, 2452
PaeI	GCATGC	1 cut(s)	2529
PagI	TCATGA	1 cut(s)	2613
PcsI	WCGNNNNNNNCGW	1 cut(s)	3026
PctI	GAATGC	3 cut(s)	485, 1271, 1373
PdmI	GAANNNNTTC	2 cut(s)	72, 1799
PflFI	GACNNNGTC	1 cut(s)	131
PflMI	CCANNNNNTGG	3 cut(s)	680, 740, 1702
PfoI	TCCNGGA	1 cut(s)	2420
PinAI	ACCGGT	1 cut(s)	198
PleI	GAGTC	7 cut(s)	100, 504, 540, 1653, 2291, 2524, 3138
PluTI	GGCGCC	4 cut(s)	687, 1247, 2088, 3326
PpsI	GAGTC	7 cut(s)	100, 504, 540, 1653, 2291, 2524, 3138
Ppu21I	YACGTR	1 cut(s)	1410
PpuMI	RGGWCCY	3 cut(s)	298, 858, 1125
PshBI	ATTAAT	1 cut(s)	2714
Psp1406I	AACGTT	1 cut(s)	518
Psp5II	RGGWCCY	3 cut(s)	298, 858, 1125
Psp6I	CCWGG	7 cut(s)	135, 739, 790, 811, 1612, 2121, 2346
PspEI	GGTNACC	1 cut(s)	2759
PspFI	CCCAGC	3 cut(s)	490, 752, 3203
PspGI	CCWGG	7 cut(s)	135, 739, 790, 811, 1612, 2121, 2346
PspPI	GGNCC	7 cut(s)	127, 298, 665, 710, 858, 1125, 3341
PspPPI	RGGWCCY	3 cut(s)	298, 858, 1125
PstNI	CAGNNNCTG	2 cut(s)	1113, 2825
PsuI	RGATCY	3 cut(s)	629, 1031, 2263
PsyI	GACNNNGTC	1 cut(s)	131
PvuII	CAGCTG	5 cut(s)	836, 896, 1868, 2510, 2897
RsaI	GTAC	7 cut(s)	398, 1018, 1143, 2173, 2333, 2808, 2993
RsaNI	GTAC	7 cut(s)	397, 1017, 1142, 2172, 2332, 2807, 2992
RseI	CAYNNNNRTG	4 cut(s)	792, 1353, 1631, 2452
Sau3AI	GATC	8 cut(s)	33, 342, 601, 629, 1031, 1589, 2263, 2947
Sau96I	GGNCC	7 cut(s)	127, 298, 665, 710, 858, 1125, 3341
SchI	GAGTC	7 cut(s)	101, 504, 541, 1653, 2291, 2525, 3138
SduI	GDGCHC	5 cut(s)	706, 909, 1628, 2049, 3020
SfcI	CTRYAG	3 cut(s)	864, 1446, 1938
SfoI	GGCGCC	4 cut(s)	685, 1245, 2086, 3324
SinI	GGWCC	6 cut(s)	127, 298, 665, 710, 858, 1125
SmaI	CCCGGG	1 cut(s)	1130
SmiMI	CAYNNNNRTG	4 cut(s)	792, 1353, 1631, 2452
SmlI	CTYRAG	5 cut(s)	83, 302, 2667, 3139, 3330
SmoI	CTYRAG	5 cut(s)	83, 302, 2667, 3139, 3330
SphI	GCATGC	1 cut(s)	2529
SsiI	CCGC	7 cut(s)	1176, 1671, 1884, 2261, 2640, 3195, 3372
SspDI	GGCGCC	4 cut(s)	683, 1243, 2084, 3322
SspMI	CTAG	6 cut(s)	828, 1082, 1896, 1902, 2168, 3369
StyI	CCWWGG	3 cut(s)	706, 854, 1002
TaaI	ACNGT	9 cut(s)	42, 73, 1188, 1464, 1932, 1955, 2152, 2451, 3110
TaiI	ACGT	4 cut(s)	521, 1412, 2131, 2559
TaqI	TCGA	1 cut(s)	3154
TatI	WGTACW	2 cut(s)	2331, 2806
TauI	GCSGC	1 cut(s)	3375
TscAI	CASTG	5 cut(s)	934, 1935, 1960, 3049, 3282
TseFI	GTSAC	4 cut(s)	938, 1926, 2759, 3104
Tsp45I	GTSAC	4 cut(s)	938, 1926, 2759, 3104
TspGWI	ACGGA	3 cut(s)	414, 642, 2224
TspMI	CCCGGG	1 cut(s)	1128
TspRI	CASTG	5 cut(s)	934, 1935, 1960, 3049, 3282
Tth111I	GACNNNGTC	1 cut(s)	131
Van91I	CCANNNNNTGG	3 cut(s)	680, 740, 1702
Vha464I	CTTAAG	2 cut(s)	302, 3139
VneI	GTGCAC	1 cut(s)	1624
VpaK11BI	GGWCC	6 cut(s)	127, 298, 665, 710, 858, 1125
VspI	ATTAAT	1 cut(s)	2714
XagI	CCTNNNNNAGG	1 cut(s)	1001
XapI	RAATTY	2 cut(s)	1820, 2247
XceI	RCATGY	2 cut(s)	2529, 3284
XcmI	CCANNNNNNNNNTGG	3 cut(s)	137, 2344, 3211
XmaI	CCCGGG	1 cut(s)	1128
XmiI	GTMKAC	3 cut(s)	1466, 2519, 3082
XmnI	GAANNNNTTC	2 cut(s)	72, 1799
XspI	CTAG	6 cut(s)	828, 1082, 1896, 1902, 2168, 3369
Zsp2I	ATGCAT	2 cut(s)	969, 2531

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.