Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1A

Orthogroup ID

OG0002550

Physical Location & Seq

Genomic Coordinates Reverse (-)

67743596 .. 67746778

Gene Structure 3183 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1AG459300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1386 bp

ATGAACGCTGGTTTACGCTTGATAAGGATGTTTGAGGGCAAAGCTTTGAAGAGAAACCGAGGCACTACTGAGCCCATCAACACCAAGCCTTTGATTGGCTATGCAGTTGTAGGCCCCATTGGGAATGTTTCTAACACTGTTGCTTCAACCCTGGAACGCGCTGATAGAATCCAACCTGCTGTTTCTGTCAGTAGTCTGGGTAATATGGAGAACAGCGGGCTTGTTGATGTTAAACCATGGATCCCTGATGTTATAGACAAGATTAAGAGATGGAAAGTTTCTGATATTGCGGATCCTTCTCAAATGAAAGCTTTAAGATTGCCCGACTCTAAAACAGCTGGAAAGGTTGTGAGACTAATGCATATTTGGAAATGGCCGCGTGATGACAGGAATAATCCATCAGGAAAGGCTAGTGCATTTGTTGTTCCACAGTTGTGGCAGCCTCGGAATGGAATTCTTATGGCTAATGATGTAAATGACAATAAGCCTGCTGAGGAATATACTGCATGCATTGCTGTATATAAAAATGATTCTTACATGATGTCTGCATCTGGCGGTAAGGTCTCTTTGTTCAACATGATGACATTTAAGGTCATGAAAACATTTGTGTCTCCTCCTCCTGCTGCCACTTTTCTGGCATTCCATCCTCAAAATAATAATATAATTGCTATTGGCATGGAGGACTCTACTATTTTAATATATAATATCAGGGTTGATGAGGTCGAAACCAAACTGAAGGGTCACTGGAATCGGATAACGGGCCTTGCGTTTTCCCAAACTCTTAACATACTGGTTTCTTCAGGAGCTGATGCCCAGTTATGTGTGTGGAGCATTTTTGGGTGGGAGAAAAAGAAAACAACGTTAATACAAGCACCAACTGATCGGCAATCCCCTTTGGTTGGAGAAACAAAAATTCAGTTTCATAACGATCACACACAATTGTTGGTTGCCCATGAAAGCCAAATTGCTGTGTATGACAGCAAGCTTGATTGTTTACGCTCTTGGACTCCCAAAGATGCACTTGCTGCTCCCATCTCATGTGCAATATATTCATGCGATGGTTTGTTGGTTTATGCTACTTTTTGTGACGGTGCTGTGGGAGTTTTTGATGCTAATAGCTTAAGACTCAGATGTCGAATAGTGCCTTCTGCTTATATACCATCATTTTCTCTCAGCGGTGGCAACCCCTCCTATCCTCTGGTTGTAGCAGCTCATCCATCTGAGCCTAACCAGATTGCAGTTGGCATGACTGACGGGTCAGTCCATGTGGTTGAACCATCTGATGCAGAGCTGAAGTGGGGTGGCACACCCTCTCAAGATAATGGTCCTTCCAATTCATCAAATCATTCTCTTAGTAGTCAAGCATCAGAACTTCCTTCCAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003002 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009753 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009867 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009933 GO:0009987 GO:0010014 GO:0010016 GO:0010033 GO:0010051 GO:0010072 GO:0010154 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032870 GO:0042221 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045892 GO:0045934 GO:0046983 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048519 GO:0048523 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0080090 GO:0090421 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

461

Amino Acids

50.32

Weight (kDa)

8.55

Isoelectric Point (pI)

44.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR3_1st	PF25173	146 - 278	2.2e-15	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	158 - 280	5.3e-13	CDC20/Fizzy WD40 domain
WD40_WDHD1_1st	PF24817	162 - 373	1.3e-18	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	167 - 281	2.1e-08	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd	PF25172	170 - 286	5.2e-10	WDR3 second beta-propeller domain
Beta-prop_WDR5	PF25175	171 - 423	1.1e-15	WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	179 - 278	1.5e-09	WDR36/Utp21 second beta-propeller domain
Beta-prop_IFT140_1st	PF23383	181 - 278	1.3e-10	IFT140 first beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	182 - 278	1e-11	WDR36/Utp21 first beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	187 - 279	4.5e-08	WDR90/POC16, second beta-propeller
Beta-prop_RIG_1st	PF23770	195 - 260	1.5e-06	RIG first beta-propeller
WD40_Prp19	PF24814	206 - 373	8.2e-14	Prp19 WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0002550)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770
malus_domestica	MD01G1234100.v1.1 MD07G1306300.v1.1
prunus_persica	Prupe.2G324300_v2.0.a1
pyrus_communis	pycom01g24260 pycom07g27930
rosa_chinensis	RchiOBHm_Chr1g0382471 RchiOBHm_Chr1g0382481 RchiOBHm_Chr1g0383911
rosa_laevigata	RLG00000026087 RLG00000026174 RLG00000026177
rosa_multiflora	Rmu_sc0000110.1_g000051 Rmu_sc0003885.1_g000009 Rmu_sc0005139.1_g000028
rosa_roxburghii	Rroxscaffold_4G00277160 Rroxscaffold_4G00278020 Rroxscaffold_4G00278080
rosa_rugosa	Rorug01G0434600 Rorug01G0434900 Rorug01G0443000
rosa_samantha	Rh1AG459200 Rh1AG459300 Rh1AG459600 Rh1AG468900 Rh1BG415700 Rh1BG426600 Rh1CG429700 Rh1CG429800 Rh1CG439400 Rh1DG447800 Rh1DG447900 Rh1DG459400
rosa_wichuraiana	Rw1G040350 Rw1G041180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	1131, 1255, 1259
Acc36I	ACCTGC	1 cut(s)	184
AccII	CGCG	2 cut(s)	159, 379
AciI	CCGC	5 cut(s)	216, 290, 377, 555, 1176
AclI	AACGTT	1 cut(s)	860
AclWI	GGATC	4 cut(s)	235, 248, 287, 300
AcoI	YGGCCR	1 cut(s)	374
AcsI	RAATTY	2 cut(s)	453, 912
AcuI	CTGAAG	3 cut(s)	755, 783, 1313
AfiI	CCNNNNNNNGG	3 cut(s)	95, 449, 899
AflII	CTTAAG	1 cut(s)	1120
AgsI	TTSAA	4 cut(s)	49, 147, 574, 1274
AjnI	CCWGG	2 cut(s)	150, 1379
AleI	CACNNNNGTG	1 cut(s)	433
AluBI	AGCT	8 cut(s)	44, 311, 338, 806, 985, 1119, 1211, 1291
AluI	AGCT	8 cut(s)	44, 311, 338, 806, 985, 1119, 1211, 1291
Alw26I	GTCTC	3 cut(s)	346, 568, 615
AlwI	GGATC	4 cut(s)	235, 248, 287, 300
AlwNI	CAGNNNCTG	1 cut(s)	806
AoxI	GGCC	3 cut(s)	112, 374, 760
ApeKI	GCWGC	4 cut(s)	439, 623, 1025, 1208
ApoI	RAATTY	2 cut(s)	453, 912
AspLEI	GCGC	1 cut(s)	161
AspS9I	GGNCC	3 cut(s)	113, 760, 1325
AvaII	GGWCC	1 cut(s)	1325
BamHI	GGATCC	2 cut(s)	240, 292
BanII	GRGCYC	1 cut(s)	75
BbvCI	CCTCAGC	1 cut(s)	492
BbvI	GCAGC	4 cut(s)	451, 610, 1012, 1220
BccI	CCATC	9 cut(s)	83, 264, 406, 651, 1040, 1052, 1168, 1225, 1285
BciT130I	CCWGG	2 cut(s)	152, 1381
BcoDI	GTCTC	3 cut(s)	346, 568, 615
BfaI	CTAG	1 cut(s)	411
BfrI	CTTAAG	1 cut(s)	1120
BfuAI	ACCTGC	1 cut(s)	184
BisI	GCNGC	5 cut(s)	377, 440, 624, 1026, 1209
BlsI	GCNGC	5 cut(s)	378, 441, 625, 1027, 1210
Bme1390I	CCNGG	2 cut(s)	152, 1381
Bme18I	GGWCC	1 cut(s)	1325
BmgT120I	GGNCC	3 cut(s)	113, 760, 1325
BmiI	GGNNCC	3 cut(s)	115, 242, 294
BmrFI	CCNGG	2 cut(s)	152, 1381
BmrI	ACTGGG	1 cut(s)	808
BmsI	GCATC	6 cut(s)	557, 799, 1006, 1099, 1273, 1373
BmuI	ACTGGG	1 cut(s)	808
Bpu10I	CCTNAGC	1 cut(s)	492
BpuEI	CTTGAG	1 cut(s)	1299
BsaI	GGTCTC	1 cut(s)	568
BsaJI	CCNNGG	4 cut(s)	58, 150, 236, 443
Bsc4I	CCNNNNNNNGG	3 cut(s)	95, 449, 899
Bse1I	ACTGG	3 cut(s)	749, 795, 814
Bse3DI	GCAATG	1 cut(s)	510
BseBI	CCWGG	2 cut(s)	152, 1381
BseDI	CCNNGG	4 cut(s)	58, 150, 236, 443
BseGI	GGATG	3 cut(s)	33, 643, 1213
BseLI	CCNNNNNNNGG	3 cut(s)	95, 449, 899
BseMI	GCAATG	1 cut(s)	510
BseMII	CTCAG	5 cut(s)	60, 483, 1141, 1186, 1212
BseNI	ACTGG	3 cut(s)	749, 795, 814
BseRI	GAGGAG	2 cut(s)	603, 606
BseXI	GCAGC	4 cut(s)	451, 610, 1012, 1220
Bsh1236I	CGCG	2 cut(s)	159, 379
BshFI	GGCC	3 cut(s)	114, 376, 762
BslI	CCNNNNNNNGG	3 cut(s)	95, 449, 899
BsmAI	GTCTC	3 cut(s)	346, 568, 615
BsmI	GAATGC	1 cut(s)	638
BsnI	GGCC	3 cut(s)	114, 376, 762
Bso31I	GGTCTC	1 cut(s)	568
Bsp1286I	GDGCHC	1 cut(s)	75
Bsp143I	GATC	4 cut(s)	240, 292, 880, 928
Bsp19I	CCATGG	1 cut(s)	236
BspACI	CCGC	5 cut(s)	216, 290, 377, 555, 1176
BspANI	GGCC	3 cut(s)	114, 376, 762
BspCNI	CTCAG	5 cut(s)	61, 484, 1140, 1185, 1213
BspFNI	CGCG	2 cut(s)	159, 379
BspHI	TCATGA	1 cut(s)	594
BspLI	GGNNCC	3 cut(s)	115, 242, 294
BspMI	ACCTGC	1 cut(s)	184
BspPI	GGATC	4 cut(s)	235, 248, 287, 300
BspTI	CTTAAG	1 cut(s)	1120
BspTNI	GGTCTC	1 cut(s)	568
BsrDI	GCAATG	1 cut(s)	510
BsrI	ACTGG	3 cut(s)	749, 795, 814
BssECI	CCNNGG	4 cut(s)	58, 150, 236, 443
BssMI	GATC	4 cut(s)	240, 292, 880, 928
BssT1I	CCWWGG	1 cut(s)	236
Bst2UI	CCWGG	2 cut(s)	152, 1381
Bst4CI	ACNGT	3 cut(s)	139, 432, 1091
Bst6I	CTCTTC	1 cut(s)	44
BstAFI	CTTAAG	1 cut(s)	1120
BstAPI	GCANNNNNTGC	2 cut(s)	512, 1025
BstC8I	GCNNGC	4 cut(s)	218, 489, 508, 983
BstDEI	CTNAG	6 cut(s)	69, 492, 1127, 1172, 1221, 1352
BstDSI	CCRYGG	1 cut(s)	236
BstF5I	GGATG	3 cut(s)	33, 643, 1213
BstFNI	CGCG	2 cut(s)	159, 379
BstHHI	GCGC	1 cut(s)	161
BstKTI	GATC	4 cut(s)	243, 295, 883, 931
BstMAI	GTCTC	3 cut(s)	346, 568, 615
BstMBI	GATC	4 cut(s)	240, 292, 880, 928
BstMWI	GCNNNNNNNGC	2 cut(s)	512, 1025
BstNI	CCWGG	2 cut(s)	152, 1381
BstNSI	RCATGY	1 cut(s)	510
BstSCI	CCNGG	2 cut(s)	150, 1379
BstUI	CGCG	2 cut(s)	159, 379
BstV1I	GCAGC	4 cut(s)	451, 610, 1012, 1220
BstX2I	RGATCY	2 cut(s)	240, 292
BstXI	CCANNNNNNTGG	2 cut(s)	435, 634
BstYI	RGATCY	2 cut(s)	240, 292
BsuRI	GGCC	3 cut(s)	114, 376, 762
BtgI	CCRYGG	1 cut(s)	236
BtgZI	GCGATG	1 cut(s)	1071
BtsCI	GGATG	3 cut(s)	33, 643, 1213
BtsIMutI	CAGTG	2 cut(s)	135, 742
BveI	ACCTGC	1 cut(s)	184
Cac8I	GCNNGC	4 cut(s)	218, 489, 508, 983
CaiI	CAGNNNCTG	1 cut(s)	806
CciI	TCATGA	1 cut(s)	594
CfoI	GCGC	1 cut(s)	161
Cfr13I	GGNCC	3 cut(s)	113, 760, 1325
DdeI	CTNAG	6 cut(s)	69, 492, 1127, 1172, 1221, 1352
DpnI	GATC	4 cut(s)	242, 294, 882, 930
DpnII	GATC	4 cut(s)	240, 292, 880, 928
DrdI	GACNNNNNNGTC	3 cut(s)	1131, 1255, 1259
DseDI	GACNNNNNNGTC	3 cut(s)	1131, 1255, 1259
EaeI	YGGCCR	1 cut(s)	374
Eam1104I	CTCTTC	1 cut(s)	44
EarI	CTCTTC	1 cut(s)	44
Eco130I	CCWWGG	1 cut(s)	236
Eco24I	GRGCYC	1 cut(s)	75
Eco31I	GGTCTC	1 cut(s)	568
Eco47I	GGWCC	1 cut(s)	1325
Eco57I	CTGAAG	3 cut(s)	755, 783, 1313
EcoO109I	RGGNCCY	1 cut(s)	113
EcoRI	GAATTC	1 cut(s)	453
EcoRII	CCWGG	2 cut(s)	150, 1379
EcoT14I	CCWWGG	1 cut(s)	236
EcoT22I	ATGCAT	2 cut(s)	363, 512
EcoT38I	GRGCYC	1 cut(s)	75
ErhI	CCWWGG	1 cut(s)	236
FalI	AAGNNNNNCTT	2 cut(s)	1005, 1037
FauI	CCCGC	1 cut(s)	209
Fnu4HI	GCNGC	5 cut(s)	377, 440, 624, 1026, 1209
FokI	GGATG	3 cut(s)	40, 630, 1200
FriOI	GRGCYC	1 cut(s)	75
Fsp4HI	GCNGC	5 cut(s)	377, 440, 624, 1026, 1209
FspBI	CTAG	1 cut(s)	411
GlaI	GCGC	1 cut(s)	160
GluI	GCNGC	5 cut(s)	377, 440, 624, 1026, 1209
HaeIII	GGCC	3 cut(s)	114, 376, 762
HhaI	GCGC	1 cut(s)	161
Hin6I	GCGC	1 cut(s)	159
HinP1I	GCGC	1 cut(s)	159
HindIII	AAGCTT	3 cut(s)	42, 309, 983
HinfI	GANTC	7 cut(s)	168, 326, 530, 683, 748, 1006, 1125
Hpy166II	GTNNAC	2 cut(s)	14, 995
Hpy188I	TCNGA	7 cut(s)	283, 447, 753, 1130, 1222, 1282, 1369
Hpy188III	TCNNGA	4 cut(s)	402, 595, 801, 1316
Hpy8I	GTNNAC	2 cut(s)	14, 995
HpyAV	CCTTC	5 cut(s)	306, 730, 1155, 1338, 1386
HpyCH4III	ACNGT	3 cut(s)	139, 432, 1091
HpyCH4IV	ACGT	1 cut(s)	860
HpyF10VI	GCNNNNNNNGC	2 cut(s)	512, 1025
HpyF3I	CTNAG	6 cut(s)	69, 492, 1127, 1172, 1221, 1352
HpySE526I	ACGT	1 cut(s)	860
HspAI	GCGC	1 cut(s)	159
Kzo9I	GATC	4 cut(s)	240, 292, 880, 928
LmnI	GCTCC	3 cut(s)	803, 828, 1033
Lsp1109I	GCAGC	4 cut(s)	451, 610, 1012, 1220
LweI	GCATC	6 cut(s)	557, 799, 1006, 1099, 1273, 1373
MaeI	CTAG	1 cut(s)	411
MaeII	ACGT	1 cut(s)	860
MaeIII	GTNAC	2 cut(s)	740, 1085
MalI	GATC	4 cut(s)	242, 294, 882, 930
MboI	GATC	4 cut(s)	240, 292, 880, 928
MboII	GAAGA	2 cut(s)	61, 789
MfeI	CAATTG	1 cut(s)	938
MflI	RGATCY	2 cut(s)	240, 292
MhlI	GDGCHC	1 cut(s)	75
MluCI	AATT	6 cut(s)	453, 663, 912, 938, 963, 1333
MlyI	GAGTC	4 cut(s)	320, 677, 1000, 1119
MmeI	TCCRAC	2 cut(s)	196, 880
Mph1103I	ATGCAT	2 cut(s)	363, 512
MseI	TTAA	8 cut(s)	231, 264, 314, 588, 695, 783, 863, 1121
MslI	CAYNNNNRTG	1 cut(s)	433
MspA1I	CMGCKG	3 cut(s)	216, 338, 1176
MspCI	CTTAAG	1 cut(s)	1120
MspR9I	CCNGG	2 cut(s)	152, 1381
MunI	CAATTG	1 cut(s)	938
Mva1269I	GAATGC	1 cut(s)	638
MvaI	CCWGG	2 cut(s)	152, 1381
MvnI	CGCG	2 cut(s)	159, 379
MwoI	GCNNNNNNNGC	2 cut(s)	512, 1025
NcoI	CCATGG	1 cut(s)	236
NdeII	GATC	4 cut(s)	240, 292, 880, 928
NlaIV	GGNNCC	3 cut(s)	115, 242, 294
NmuCI	GTSAC	2 cut(s)	740, 1085
NsiI	ATGCAT	2 cut(s)	363, 512
NspI	RCATGY	1 cut(s)	510
OliI	CACNNNNGTG	1 cut(s)	433
PaeI	GCATGC	1 cut(s)	510
PagI	TCATGA	1 cut(s)	594
PcsI	WCGNNNNNNNCGW	1 cut(s)	764
PctI	GAATGC	1 cut(s)	638
PfeI	GAWTC	3 cut(s)	168, 530, 748
PkrI	GCNGC	5 cut(s)	378, 441, 625, 1027, 1210
PleI	GAGTC	4 cut(s)	320, 677, 1000, 1119
PpsI	GAGTC	4 cut(s)	320, 677, 1000, 1119
Psp1406I	AACGTT	1 cut(s)	860
Psp6I	CCWGG	2 cut(s)	150, 1379
PspGI	CCWGG	2 cut(s)	150, 1379
PspN4I	GGNNCC	3 cut(s)	115, 242, 294
PspPI	GGNCC	3 cut(s)	113, 760, 1325
PstNI	CAGNNNCTG	1 cut(s)	806
PsuI	RGATCY	2 cut(s)	240, 292
PvuII	CAGCTG	1 cut(s)	338
RseI	CAYNNNNRTG	1 cut(s)	433
SaqAI	TTAA	8 cut(s)	231, 264, 314, 588, 695, 783, 863, 1121
SatI	GCNGC	5 cut(s)	377, 440, 624, 1026, 1209
Sau3AI	GATC	4 cut(s)	240, 292, 880, 928
Sau96I	GGNCC	3 cut(s)	113, 760, 1325
SchI	GAGTC	4 cut(s)	320, 677, 1000, 1119
ScrFI	CCNGG	2 cut(s)	152, 1381
SduI	GDGCHC	1 cut(s)	75
SfaNI	GCATC	6 cut(s)	557, 799, 1006, 1099, 1273, 1373
SinI	GGWCC	1 cut(s)	1325
SmiMI	CAYNNNNRTG	1 cut(s)	433
SmlI	CTYRAG	2 cut(s)	1120, 1314
SmoI	CTYRAG	2 cut(s)	1120, 1314
SphI	GCATGC	1 cut(s)	510
Sse9I	AATT	6 cut(s)	453, 663, 912, 938, 963, 1333
SsiI	CCGC	5 cut(s)	216, 290, 377, 555, 1176
SspMI	CTAG	1 cut(s)	411
StyD4I	CCNGG	2 cut(s)	150, 1379
StyI	CCWWGG	1 cut(s)	236
TaaI	ACNGT	3 cut(s)	139, 432, 1091
TaiI	ACGT	1 cut(s)	863
TaqI	TCGA	2 cut(s)	723, 1135
TasI	AATT	6 cut(s)	453, 663, 912, 938, 963, 1333
TauI	GCSGC	1 cut(s)	379
TfiI	GAWTC	3 cut(s)	168, 530, 748
Tru1I	TTAA	8 cut(s)	231, 264, 314, 588, 695, 783, 863, 1121
Tru9I	TTAA	8 cut(s)	231, 264, 314, 588, 695, 783, 863, 1121
TscAI	CASTG	2 cut(s)	142, 749
TseFI	GTSAC	2 cut(s)	740, 1085
TseI	GCWGC	4 cut(s)	439, 623, 1025, 1208
Tsp45I	GTSAC	2 cut(s)	740, 1085
TspDTI	ATGAA	7 cut(s)	17, 320, 611, 911, 969, 1041, 1326
TspRI	CASTG	2 cut(s)	142, 749
Vha464I	CTTAAG	1 cut(s)	1120
VpaK11BI	GGWCC	1 cut(s)	1325
XapI	RAATTY	2 cut(s)	453, 912
XceI	RCATGY	1 cut(s)	510
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1238
XspI	CTAG	1 cut(s)	411
Zsp2I	ATGCAT	2 cut(s)	363, 512

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.