Rorug01G0443000

Serine threonine-protein phosphatase 4 regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002550
Physical Location & Seq
Reverse (-)
54012660 .. 54014845
2186 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0443000.1

Sequence Viewer

Length: 408 bp
ATGGTGGAGGTGAGAGTTCCAAATCTTGACTGTCAGGGATGTGCTTCCAAGTTAAAGAAAGCTCTCTTCAAGCTCAAAGGTGTCGAAGTAGTGGAAATAGAGATGGAGATCCAAAAGATAAGCGTGAGAGGGTACGCGTTGGATGAAAGGAAAGTGCTAAAGGCAATTAAGCGAGCTGGAAAAGCAGTGGAGCCATGGCCATTTCCTGGTTACTCACATTATGCCTCATTTTACAAGTACCCTACATACATTAAGAACCATTACTACGACACCTACAAAAACGAAACCACCACCGGTGTACACACCTTCTTCCACACTCCGGCAGTTTATTCAGTCGCCGTGGCGTCCGATGAGGCCATTGCTTCACTCTTTAGTGATGACAATCCACATGCTTGCACTATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPP4R2

Protein Analysis

135

Amino Acids

15.29

Weight (kDa)

8.36

Isoelectric Point (pI)

33.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 4 - 61 3.4e-11 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 206
AccII CGCG 1 cut(s) 137
AclWI GGATC 1 cut(s) 103
AcoI YGGCCR 1 cut(s) 197
AcyI GRCGYC 1 cut(s) 344
AfaI GTAC 3 cut(s) 134, 239, 300
AfiI CCNNNNNNNGG 2 cut(s) 206, 319
AflIII ACRYGT 1 cut(s) 135
AgeI ACCGGT 1 cut(s) 293
AgsI TTSAA 1 cut(s) 70
AjnI CCWGG 1 cut(s) 205
AluBI AGCT 3 cut(s) 62, 73, 176
AluI AGCT 3 cut(s) 62, 73, 176
AlwI GGATC 1 cut(s) 103
AoxI GGCC 2 cut(s) 197, 354
AsiGI ACCGGT 1 cut(s) 293
AsuHPI GGTGA 1 cut(s) 22
BalI TGGCCA 1 cut(s) 199
BccI CCATC 1 cut(s) 97
BceAI ACGGC 1 cut(s) 323
BciT130I CCWGG 1 cut(s) 207
Bme1390I CCNGG 1 cut(s) 207
BmiI GGNNCC 1 cut(s) 192
BmrFI CCNGG 1 cut(s) 207
BsaBI GATNNNNATC 2 cut(s) 107, 381
BsaHI GRCGYC 1 cut(s) 344
BsaJI CCNNGG 2 cut(s) 194, 339
BsaWI WCCGGW 1 cut(s) 293
Bsc4I CCNNNNNNNGG 2 cut(s) 206, 319
Bse118I RCCGGY 1 cut(s) 293
Bse3DI GCAATG 1 cut(s) 357
Bse8I GATNNNNATC 2 cut(s) 107, 381
BseBI CCWGG 1 cut(s) 207
BseDI CCNNGG 2 cut(s) 194, 339
BseGI GGATG 2 cut(s) 44, 148
BseJI GATNNNNATC 2 cut(s) 107, 381
BseLI CCNNNNNNNGG 2 cut(s) 206, 319
BseMI GCAATG 1 cut(s) 357
Bsh1236I CGCG 1 cut(s) 137
BshFI GGCC 2 cut(s) 199, 356
BshTI ACCGGT 1 cut(s) 293
BsiSI CCGG 2 cut(s) 294, 320
BslI CCNNNNNNNGG 2 cut(s) 206, 319
BsnI GGCC 2 cut(s) 199, 356
Bsp1407I TGTACA 1 cut(s) 298
Bsp143I GATC 1 cut(s) 108
Bsp19I CCATGG 1 cut(s) 194
BspANI GGCC 2 cut(s) 199, 356
BspFNI CGCG 1 cut(s) 137
BspLI GGNNCC 1 cut(s) 192
BspPI GGATC 1 cut(s) 103
BsrDI GCAATG 1 cut(s) 357
BsrFI RCCGGY 1 cut(s) 293
BsrGI TGTACA 1 cut(s) 298
BssAI RCCGGY 1 cut(s) 293
BssECI CCNNGG 2 cut(s) 194, 339
BssMI GATC 1 cut(s) 108
BssNI GRCGYC 1 cut(s) 344
BssT1I CCWWGG 1 cut(s) 194
Bst2UI CCWGG 1 cut(s) 207
Bst4CI ACNGT 1 cut(s) 32
Bst6I CTCTTC 1 cut(s) 71
BstACI GRCGYC 1 cut(s) 344
BstAUI TGTACA 1 cut(s) 298
BstC8I GCNNGC 2 cut(s) 174, 394
BstDSI CCRYGG 2 cut(s) 194, 339
BstF5I GGATG 2 cut(s) 44, 148
BstFNI CGCG 1 cut(s) 137
BstKTI GATC 1 cut(s) 111
BstMBI GATC 1 cut(s) 108
BstMWI GCNNNNNNNGC 1 cut(s) 182
BstNI CCWGG 1 cut(s) 207
BstNSI RCATGY 1 cut(s) 392
BstSCI CCNGG 1 cut(s) 205
BstUI CGCG 1 cut(s) 137
BstX2I RGATCY 1 cut(s) 108
BstYI RGATCY 1 cut(s) 108
BsuRI GGCC 2 cut(s) 199, 356
BtgI CCRYGG 2 cut(s) 194, 339
BtsCI GGATG 2 cut(s) 44, 148
BtsI GCAGTG 1 cut(s) 192
BtsIMutI CAGTG 1 cut(s) 192
Cac8I GCNNGC 2 cut(s) 174, 394
Cfr10I RCCGGY 1 cut(s) 293
CseI GACGC 1 cut(s) 333
Csp6I GTAC 3 cut(s) 133, 238, 299
CspAI ACCGGT 1 cut(s) 293
CviAII CATG 2 cut(s) 195, 389
CviJI RGCY 6 cut(s) 62, 73, 176, 193, 199, 356
CviKI_1 RGCY 6 cut(s) 62, 73, 176, 193, 199, 356
CviQI GTAC 3 cut(s) 133, 238, 299
DpnI GATC 1 cut(s) 110
DpnII GATC 1 cut(s) 108
EaeI YGGCCR 1 cut(s) 197
Eam1104I CTCTTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 71
Eco130I CCWWGG 1 cut(s) 194
EcoRII CCWGG 1 cut(s) 205
EcoT14I CCWWGG 1 cut(s) 194
ErhI CCWWGG 1 cut(s) 194
FaeI CATG 2 cut(s) 198, 392
FaiI YATR 5 cut(s) 196, 222, 247, 390, 404
FatI CATG 2 cut(s) 194, 388
FokI GGATG 2 cut(s) 51, 155
HaeIII GGCC 2 cut(s) 199, 356
HapII CCGG 2 cut(s) 294, 320
HgaI GACGC 1 cut(s) 333
Hin1I GRCGYC 1 cut(s) 344
Hin1II CATG 2 cut(s) 198, 392
HpaII CCGG 2 cut(s) 294, 320
HphI GGTGA 1 cut(s) 22
Hpy166II GTNNAC 2 cut(s) 299, 301
Hpy188I TCNGA 1 cut(s) 349
Hpy188III TCNNGA 1 cut(s) 26
Hpy8I GTNNAC 2 cut(s) 299, 301
HpyAV CCTTC 1 cut(s) 316
HpyCH4III ACNGT 1 cut(s) 32
HpyCH4V TGCA 1 cut(s) 396
HpyF10VI GCNNNNNNNGC 1 cut(s) 182
Hsp92I GRCGYC 1 cut(s) 344
Hsp92II CATG 2 cut(s) 198, 392
Kzo9I GATC 1 cut(s) 108
LmnI GCTCC 1 cut(s) 190
LpnPI CCDG 6 cut(s) 20, 162, 192, 219, 307, 333
MaeIII GTNAC 1 cut(s) 209
MalI GATC 1 cut(s) 110
MboI GATC 1 cut(s) 108
MboII GAAGA 2 cut(s) 58, 301
MflI RGATCY 1 cut(s) 108
MlsI TGGCCA 1 cut(s) 199
MluCI AATT 1 cut(s) 165
MluI ACGCGT 1 cut(s) 135
MluNI TGGCCA 1 cut(s) 199
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 3 cut(s) 122, 235, 346
Mox20I TGGCCA 1 cut(s) 199
MscI TGGCCA 1 cut(s) 199
MseI TTAA 3 cut(s) 53, 168, 252
MslI CAYNNNNRTG 1 cut(s) 401
Msp20I TGGCCA 1 cut(s) 199
MspI CCGG 2 cut(s) 294, 320
MspR9I CCNGG 1 cut(s) 207
MvaI CCWGG 1 cut(s) 207
MvnI CGCG 1 cut(s) 137
MwoI GCNNNNNNNGC 1 cut(s) 182
NcoI CCATGG 1 cut(s) 194
NdeII GATC 1 cut(s) 108
NlaIII CATG 2 cut(s) 198, 392
NlaIV GGNNCC 1 cut(s) 192
NspI RCATGY 1 cut(s) 392
PflMI CCANNNNNTGG 1 cut(s) 206
PinAI ACCGGT 1 cut(s) 293
Psp6I CCWGG 1 cut(s) 205
PspGI CCWGG 1 cut(s) 205
PspN4I GGNNCC 1 cut(s) 192
PsrI GAACNNNNNNTAC 2 cut(s) 248, 280
PsuI RGATCY 1 cut(s) 108
RsaI GTAC 3 cut(s) 134, 239, 300
RsaNI GTAC 3 cut(s) 133, 238, 299
RseI CAYNNNNRTG 1 cut(s) 401
SaqAI TTAA 3 cut(s) 53, 168, 252
Sau3AI GATC 1 cut(s) 108
ScrFI CCNGG 1 cut(s) 207
SetI ASST 7 cut(s) 12, 64, 75, 82, 178, 275, 308
SgrAI CRCCGGYG 1 cut(s) 293
SmiMI CAYNNNNRTG 1 cut(s) 401
Sse9I AATT 1 cut(s) 165
StyD4I CCNGG 1 cut(s) 205
StyI CCWWGG 1 cut(s) 194
TaaI ACNGT 1 cut(s) 32
TaqI TCGA 1 cut(s) 84
TasI AATT 1 cut(s) 165
TatI WGTACW 1 cut(s) 298
Tru1I TTAA 3 cut(s) 53, 168, 252
Tru9I TTAA 3 cut(s) 53, 168, 252
TscAI CASTG 1 cut(s) 192
TspDTI ATGAA 1 cut(s) 159
TspRI CASTG 1 cut(s) 192
Van91I CCANNNNNTGG 1 cut(s) 206
XceI RCATGY 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.