Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0002550

Physical Location & Seq

Genomic Coordinates Forward (+)

68031614 .. 68040099

Gene Structure 8486 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ60679

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3411 bp

ATGTCTTCCTTGAGTAGGGAACTGGTGTTCTTGATTTTACAGTTCTTGGACGAGGAGAAGTTCAAGGAAACAGTTCATAAGCTAGAGCAAGAGTCTGGCTTTTTCTTCAATATGAAGCATTTTGAGGATCAAGTCCAGGCCGGAGAGTGGGACGAAGTCGAGCGATATTTGGGTGGCTTCACTAAAGTTGAAGACAATCGGTATTCAATGAAGATCTTCTTTGAAATCAGGAAGCAGAAGTATTTGGAAGCTCTCGATAGGCAGGATAGGGCGAAGGCTGTTGAGATTCTTGTGAAGGACCTTAAGGTTTTTGCATCTTTTAATGAGGAACTTTTCAAGGAGATCACTCAGTTGCTCACTCTTGACAACTTCAGGCAAAATGAGCAGCTTTCCAAGTACGGGGACACAAAATCTGCTCGGAATATCATGCTTATAGAACTTAAAAAGCTCATTGAAGCGAACCCATTATTTCGTGATAAACTTGCATTTCCGTCCTTCAAAAGTTCACGGTTAAGGACTTTGATAAACCAGAGTCTCAACTGGCAGCACCAACTTTGCAAGAATCCCCGTCCAAATCCTGATATTAAAACACTATTTATGGATCATTCTTGCACTCCAAATGCTAATGGATCTCGTCCTCCTCCCACAAACAATCCCCTTGTTGGACCAATTCCAAAGGCTGGTGCATTTCCTCCTATTGGAGCCCATGGTCCATTTCAGCCTGTTGTCTCCCCTTCTCCTGGTGCTATTGCTGGGTGGATGTCAAGCGCTAATCCTTCTATGCCTCACCCTGCTGTTGCAGCAGCTCCTCCTGGTCTTGTGCAGCCTTCTAGTGCAGCTGCTTTCTTAAAGCACCCAAGGACGCCGACAGGTGTTACAGGGATGGATTATCAATCAGCTGATTCAGAGCACTTGATTAAGCGTATTCGTACTGGCCCAGCTGATGAGGTGTCCTTTTCTGGGGTGATGCTTAGTTCCAATGCGTATTCACAAGATGACCTTCCGAAGGCTGTTGTTCGGACCCTTAGTCAAGGATCTAATGTTATGAGCATGGACTTTCATCCACTGCAACAAAATATTCTTCTAGTTGGGACAAATGTTGGCGACATCAGTCTTTGGGAATTAGGATCCCGGGAAAGGTTGGTACATAAACCTTTCAAGGTCTGGGATATACAAGCTGCGTCAATGCCACTGCAGAATGCTTTGTTGAATGATGCTGCAATATCAGTGAATCGTTGTGTTTGGAGCCCGGATGGACTTATGCTTGGTGTTGCATTTTCTAAGCACATCGTCCAGATATATGCTAATAATCCAACTGGAGAATTGAGACAACATTTGGAGATTGATGCTCACGTTGGCGGTGTTAATGACATTGCATTTGCTCATCCTAACAAGCAATTGTGTATTGTTACTTGCGGAGATGATAAAGTGATTAAGGTATGGGATGCTGCAGCCGGACGCAGACAATATACATTTGAAGGCCATGAAGCTCCTGTATATTCAGTGTGCCCTCATTACAAAGAAAGTATACAATTCATTTTTTCTACCGCGATTGATGGGAAGATTAAAGCTTGGCTATACGATTGTTTGGGGTCAAGGGTGGACTATGATGCTCCTGGACTTTGGTGCACCATGATGGCTTATAGTGCAGATGGAACCAGACTCTTCTCATGTGGAACGAGTAAAGAAGGTGAATCTCATCTGGTGGAGTGGAATGAGAGCGAAGGGGCCATCAAACGGACATATTCTGGGTTTAGAAAACGCTCTTTGGATGTTGTCCAGTTTGACACAACAAGGAACCGGTTCCTAGCCGCTGGTGATGAATTTCAAATAAAGTTCTGGGATATGGACAATACCAATGTGCTGACAGCTGTTGATGCAGATGGCGGGTTGCCTGCTAGTCCTAGACTCAGATTCAACAAAGAAGGTTCCTTGTTAGCTGTAACAACGACTGACAGCGGTATAAAGATTTTAGCAAATAATGATGGTTTGCGCTTGATACGGATGTTGGAGAGCAAAGCTATGGAGAGAAACCGAGGCACTTCTGAGCCCATCAACACTAAGCCTTTGATTGTCAATGCACTAGGCCCCATTGGGAATGTTTCTAACGCTGTTGCTCCAACCCTCGAACGTGCTGATAGAATCCAACCTGCTGTTTCTATCAGTAGTCTGGGTAATATGGAGAACAGCAGGCTTGTTGATGTTAAACCACGGATCCCTGATGATATAGACAAGATTAAGAGCTGGAAAATTTCTGATATTGCGGATCCTTCTCAAATGAAAGCTTTAAGATTGCCCGACTCTACAACAGCTGGAAAGGTTGTGAGACTAATGTACACGAATAATGGTTTGGCTCTGTTAGCCCTCGCCTCCAATGCTGTTCACAAGCTTTGGAAGTGGCCGCGTAATGACAGGAATAATGCATCAGGGAAGGCTAGTGCATATGTTGTTCCACAGTTGTGGCAGCCCCCGAATGGAATTCTGATGGCTAATGATGTAAATGACAATAAACCAGCTGAGGAATCTACTGCATGTATTGCTTTATCTAAAAATGATTCTTACGTAATGTCTGCATCTGGCGGGAAGGTCTCTTTGTTCAACATGATGACATTTAAGGTCATGACAACATTTGTATCTCCCCCTCCTGCTGCCACTTTTCTGGCATTCCATCCTCAAGATAATAATATAATTGCTATTGGCATGGAGGACTCTACTATTTTAATATATAATGTCAGGGTTGATGAGGTCAAAACCAAACTTAAGGGTCACCAGAATCGGATAACAGGCCTTGCATTTTCCCAGACTCTTAACATACTGGTCTCTTCAGGAGCTGATGCCCAGTTATGTGTGTGGAGCATTGATGGTTGGGAGAAAAAGAAAACAAGGTTCATACAAGCACCAACTGGTCGGCAATCCCCATTGGTTGGAGAAACAAAAGTTCAGTTTCATAATGATCACACACATCTATTGGTTGCCCATGAAAGCCAAATTGCTGTGTATGACAGCAAGCTTGATTGTTTACGCTTTTGGTCTCCAAAAGATGCACTTGCTGATCGCATCTCATGTGCAATATATTCATGTGATGGTTTGTTGGTTTATGCTACTTTTTGTGACGGTGCTGTGGGAGTTTTTGATGCTGATAGCTTAAGACTCAGATGTCGAATAGCGCCTTCTGCTTATATACCATCATTTTCTCTCAGCGGTGGCAACCCCTCCTATCCTCTGGTTGTAGCAGCTCATCCATCTGAGCCAAACCAGATTGCAGTTGGCATGACTGACGGGTCAGTGCATGTGGTTGAACCATCTGATGCAGAGCTGAAGTGGGGTGGCACACCCTCTCAAGATAATGGTCCTTCCAATTCATCAAATCCTTCTCCTAGTGGTCAAGCATCAGAACTACCTTCCAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003002 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009753 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009867 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009933 GO:0009987 GO:0010014 GO:0010016 GO:0010033 GO:0010051 GO:0010072 GO:0010154 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032870 GO:0042221 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045892 GO:0045934 GO:0046983 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048519 GO:0048523 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0080090 GO:0090421 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

1136

Amino Acids

125.15

Weight (kDa)

6.72

Isoelectric Point (pI)

41.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LisH_TPL	PF17814	5 - 34	6.8e-13	LisH-like dimerisation domain
TPR1-like_2nd	PF21889	37 - 161	1.9e-61	TPR1-like, CTLH-containing domain
zf_topless	PF21359	164 - 204	1.5e-25	TOPLESS, zinc finger domain
WD40_CDC20-Fz	PF24807	336 - 614	3.8e-08	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd	PF25172	347 - 562	7.6e-08	WDR3 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	387 - 560	1.4e-12	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	409 - 621	1.9e-19	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	409 - 669	1.4e-20	THOC3 beta-propeller domain
WD40_Gbeta	PF25391	410 - 616	6.1e-06	G protein beta WD-40 repeat protein
WD40_Prp19	PF24814	451 - 526	3.7e-07	Prp19 WD40 domain
WD40_WDHD1_1st	PF24817	456 - 526	4.3e-08	WDHD1 first WD40 domain
WD40_CDC20-Fz	PF24807	835 - 958	2.1e-13	CDC20/Fizzy WD40 domain
WD40_WDHD1_1st	PF24817	838 - 1048	1.6e-19	WDHD1 first WD40 domain
WDR55	PF24796	838 - 954	1.5e-06	WDR55
Beta-prop_WDR3_1st	PF25173	838 - 957	1.1e-16	WDR3 first beta-propeller domain
Beta-prop_EML_2	PF23414	839 - 959	8.1e-11	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	844 - 954	4.9e-10	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5	PF25175	844 - 1055	2.5e-15	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	845 - 962	2.3e-12	WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	856 - 955	6.5e-11	WDR36/Utp21 first beta-propeller
Beta-prop_IFT140_1st	PF23383	856 - 956	2e-11	IFT140 first beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	861 - 964	9.6e-09	WDR90/POC16, second beta-propeller
Beta-prop_RIG_1st	PF23770	870 - 936	2.5e-07	RIG first beta-propeller
WD40_Prp19	PF24814	881 - 1050	1.7e-14	Prp19 WD40 domain
WDR55	PF24796	887 - 1050	1e-05	WDR55

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002550)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770
malus_domestica	MD01G1234100.v1.1 MD07G1306300.v1.1
prunus_persica	Prupe.2G324300_v2.0.a1
pyrus_communis	pycom01g24260 pycom07g27930
rosa_chinensis	RchiOBHm_Chr1g0382471 RchiOBHm_Chr1g0382481 RchiOBHm_Chr1g0383911
rosa_laevigata	RLG00000026087 RLG00000026174 RLG00000026177
rosa_multiflora	Rmu_sc0000110.1_g000051 Rmu_sc0003885.1_g000009 Rmu_sc0005139.1_g000028
rosa_roxburghii	Rroxscaffold_4G00277160 Rroxscaffold_4G00278020 Rroxscaffold_4G00278080
rosa_rugosa	Rorug01G0434600 Rorug01G0434900 Rorug01G0443000
rosa_samantha	Rh1AG459200 Rh1AG459300 Rh1AG459600 Rh1AG468900 Rh1BG415700 Rh1BG426600 Rh1CG429700 Rh1CG429800 Rh1CG439400 Rh1DG447800 Rh1DG447900 Rh1DG459400
rosa_wichuraiana	Rw1G040350 Rw1G041180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	3156, 3280
Acc36I	ACCTGC	1 cut(s)	2158
AccB7I	CCANNNNNTGG	2 cut(s)	680, 2924
AccI	GTMKAC	1 cut(s)	1528
AccII	CGCG	2 cut(s)	1550, 2404
AcoI	YGGCCR	1 cut(s)	2399
AcsI	RAATTY	3 cut(s)	1823, 2250, 2478
AcuI	CTGAAG	3 cut(s)	355, 2808, 3338
AcyI	GRCGYC	1 cut(s)	863
AfaI	GTAC	4 cut(s)	398, 931, 1146, 2336
AfeI	AGCGCT	1 cut(s)	769
AflII	CTTAAG	3 cut(s)	302, 2759, 3145
AgeI	ACCGGT	1 cut(s)	1800
AhdI	GACNNNNNGTC	1 cut(s)	1026
AjnI	CCWGG	5 cut(s)	135, 739, 811, 1615, 3404
AjuI	GAANNNNNNNTTGG	2 cut(s)	2333, 2365
AleI	CACNNNNGTG	1 cut(s)	2458
AloI	GAACNNNNNNTCC	2 cut(s)	11, 43
Alw21I	GWGCWC	2 cut(s)	912, 1631
Alw26I	GTCTC	7 cut(s)	539, 733, 1321, 2320, 2593, 2824, 3036
Alw44I	GTGCAC	1 cut(s)	1627
AlwNI	CAGNNNCTG	1 cut(s)	2831
Ama87I	CYCGRG	1 cut(s)	1131
Aor51HI	AGCGCT	1 cut(s)	769
AoxI	GGCC	7 cut(s)	138, 934, 1480, 1728, 2086, 2399, 2785
ApaLI	GTGCAC	1 cut(s)	1627
ApoI	RAATTY	3 cut(s)	1823, 2250, 2478
ArsI	GACNNNNNNTTYG	2 cut(s)	1361, 1393
AsiGI	ACCGGT	1 cut(s)	1800
Asp700I	GAANNNNTTC	3 cut(s)	72, 215, 1802
AspLEI	GCGC	3 cut(s)	770, 1995, 3169
AspS9I	GGNCC	8 cut(s)	298, 665, 710, 935, 1020, 1728, 2087, 3350
AsuC2I	CCSGG	3 cut(s)	1132, 1133, 1250
AsuHPI	GGTGA	5 cut(s)	779, 976, 1703, 1829, 2759
AvaI	CYCGRG	1 cut(s)	1131
AvaII	GGWCC	5 cut(s)	298, 665, 710, 1020, 3350
BaeGI	GKGCMC	2 cut(s)	1511, 1631
BaeI	ACNNNNGTAYC	4 cut(s)	1991, 2024, 2616, 2649
BamHI	GGATCC	3 cut(s)	1127, 2214, 2266
BanII	GRGCYC	3 cut(s)	706, 1250, 2052
BbsI	GAAGAC	1 cut(s)	198
Bbv12I	GWGCWC	2 cut(s)	912, 1631
BbvCI	CCTCAGC	1 cut(s)	2517
BciT130I	CCWGG	5 cut(s)	137, 741, 813, 1617, 3406
BclI	TGATCA	1 cut(s)	2953
BcnI	CCSGG	3 cut(s)	1132, 1133, 1250
BcoDI	GTCTC	7 cut(s)	539, 733, 1321, 2320, 2593, 2824, 3036
BfaI	CTAG	9 cut(s)	83, 831, 1085, 1808, 1899, 1905, 2084, 2436, 3378
BfmI	CTRYAG	2 cut(s)	1193, 1449
BfoI	RGCGCY	2 cut(s)	771, 3170
BfrI	CTTAAG	3 cut(s)	302, 2759, 3145
BfuAI	ACCTGC	1 cut(s)	2158
BglII	AGATCT	1 cut(s)	213
Bme1390I	CCNGG	8 cut(s)	137, 741, 813, 1132, 1133, 1250, 1617, 3406
Bme18I	GGWCC	5 cut(s)	298, 665, 710, 1020, 3350
BmeRI	GACNNNNNGTC	1 cut(s)	1026
BmeT110I	CYCGRG	1 cut(s)	1131
BmgT120I	GGNCC	8 cut(s)	298, 665, 710, 935, 1020, 1728, 2087, 3350
BmrFI	CCNGG	8 cut(s)	137, 741, 813, 1132, 1133, 1250, 1617, 3406
BmrI	ACTGGG	1 cut(s)	2833
BmuI	ACTGGG	1 cut(s)	2833
BoxI	GACNNNNGTC	1 cut(s)	1110
BpiI	GAAGAC	1 cut(s)	198
BpmI	CTGGAG	1 cut(s)	1338
Bpu10I	CCTNAGC	1 cut(s)	2517
BpuEI	CTTGAG	3 cut(s)	31, 2658, 3324
BpuMI	CCSGG	3 cut(s)	1132, 1133, 1250
BsaAI	YACGTR	1 cut(s)	2563
BsaHI	GRCGYC	1 cut(s)	863
BsaI	GGTCTC	3 cut(s)	2593, 2824, 3036
BsaJI	CCNNGG	5 cut(s)	706, 857, 1131, 2035, 2210
BsaWI	WCCGGW	1 cut(s)	1800
Bse118I	RCCGGY	1 cut(s)	1800
Bse1I	ACTGG	8 cut(s)	27, 545, 937, 1321, 1780, 2820, 2839, 2908
Bse3DI	GCAATG	1 cut(s)	1371
BseBI	CCWGG	5 cut(s)	137, 741, 813, 1617, 3406
BseDI	CCNNGG	5 cut(s)	706, 857, 1131, 2035, 2210
BseMI	GCAATG	1 cut(s)	1371
BseMII	CTCAG	7 cut(s)	362, 1924, 2037, 2508, 3166, 3211, 3237
BseNI	ACTGG	8 cut(s)	27, 545, 937, 1321, 1780, 2820, 2839, 2908
BseRI	GAGGAG	3 cut(s)	68, 630, 798
BseSI	GKGCMC	2 cut(s)	1511, 1631
BseYI	CCCAGC	2 cut(s)	752, 937
BsgI	GTGCAG	3 cut(s)	842, 855, 1668
Bsh1236I	CGCG	2 cut(s)	1550, 2404
BshFI	GGCC	7 cut(s)	140, 936, 1482, 1730, 2088, 2401, 2787
BshTI	ACCGGT	1 cut(s)	1800
BsiHKAI	GWGCWC	2 cut(s)	912, 1631
BsiHKCI	CYCGRG	1 cut(s)	1131
BsiSI	CCGG	5 cut(s)	141, 1132, 1250, 1455, 1801
BslFI	GGGAC	3 cut(s)	164, 416, 1105
BsmAI	GTCTC	7 cut(s)	539, 733, 1321, 2320, 2593, 2824, 3036
BsmFI	GGGAC	3 cut(s)	164, 416, 1105
BsmI	GAATGC	2 cut(s)	1204, 2663
BsnI	GGCC	7 cut(s)	140, 936, 1482, 1730, 2088, 2401, 2787
Bso31I	GGTCTC	3 cut(s)	2593, 2824, 3036
BsoBI	CYCGRG	1 cut(s)	1131
Bsp1286I	GDGCHC	6 cut(s)	706, 912, 1250, 1511, 1631, 2052
Bsp1407I	TGTACA	1 cut(s)	2334
Bsp19I	CCATGG	1 cut(s)	706
BspANI	GGCC	7 cut(s)	140, 936, 1482, 1730, 2088, 2401, 2787
BspCNI	CTCAG	7 cut(s)	361, 1923, 2038, 2509, 3165, 3210, 3238
BspFNI	CGCG	2 cut(s)	1550, 2404
BspHI	TCATGA	1 cut(s)	2619
BspMAI	CTGCAG	2 cut(s)	1197, 1453
BspMI	ACCTGC	1 cut(s)	2158
BspTI	CTTAAG	3 cut(s)	302, 2759, 3145
BspTNI	GGTCTC	3 cut(s)	2593, 2824, 3036
BsrDI	GCAATG	1 cut(s)	1371
BsrFI	RCCGGY	1 cut(s)	1800
BsrGI	TGTACA	1 cut(s)	2334
BsrI	ACTGG	8 cut(s)	27, 545, 937, 1321, 1780, 2820, 2839, 2908
BssAI	RCCGGY	1 cut(s)	1800
BssECI	CCNNGG	5 cut(s)	706, 857, 1131, 2035, 2210
BssNAI	GTATAC	1 cut(s)	1529
BssNI	GRCGYC	1 cut(s)	863
BssT1I	CCWWGG	2 cut(s)	706, 857
Bst1107I	GTATAC	1 cut(s)	1529
Bst2UI	CCWGG	5 cut(s)	137, 741, 813, 1617, 3406
Bst4CI	ACNGT	5 cut(s)	42, 73, 510, 2457, 3116
Bst6I	CTCTTC	2 cut(s)	1670, 2827
BstACI	GRCGYC	1 cut(s)	863
BstAFI	CTTAAG	3 cut(s)	302, 2759, 3145
BstAPI	GCANNNNNTGC	1 cut(s)	2537
BstAUI	TGTACA	1 cut(s)	2334
BstBAI	YACGTR	1 cut(s)	2563
BstC8I	GCNNGC	3 cut(s)	1896, 2192, 3008
BstDSI	CCRYGG	2 cut(s)	706, 2210
BstEII	GGTNACC	1 cut(s)	2765
BstENI	CCTNNNNNAGG	2 cut(s)	13, 1004
BstFNI	CGCG	2 cut(s)	1550, 2404
BstH2I	RGCGCY	2 cut(s)	771, 3170
BstHHI	GCGC	3 cut(s)	770, 1995, 3169
BstMAI	GTCTC	7 cut(s)	539, 733, 1321, 2320, 2593, 2824, 3036
BstMWI	GCNNNNNNNGC	8 cut(s)	382, 800, 1646, 1877, 2360, 2375, 2537, 3173
BstNI	CCWGG	5 cut(s)	137, 741, 813, 1617, 3406
BstNSI	RCATGY	2 cut(s)	2535, 3293
BstPAI	GACNNNNGTC	1 cut(s)	1110
BstPI	GGTNACC	1 cut(s)	2765
BstSCI	CCNGG	8 cut(s)	135, 739, 811, 1130, 1131, 1248, 1615, 3404
BstSFI	CTRYAG	2 cut(s)	1193, 1449
BstSLI	GKGCMC	2 cut(s)	1511, 1631
BstSNI	TACGTA	1 cut(s)	2563
BstUI	CGCG	2 cut(s)	1550, 2404
BstV2I	GAAGAC	1 cut(s)	198
BstX2I	RGATCY	6 cut(s)	213, 629, 1034, 1127, 2214, 2266
BstXI	CCANNNNNNTGG	2 cut(s)	2460, 2659
BstYI	RGATCY	6 cut(s)	213, 629, 1034, 1127, 2214, 2266
BstZ17I	GTATAC	1 cut(s)	1529
BsuRI	GGCC	7 cut(s)	140, 936, 1482, 1730, 2088, 2401, 2787
BtgI	CCRYGG	2 cut(s)	706, 2210
BtsI	GCAGTG	2 cut(s)	1064, 1190
BtsIMutI	CAGTG	5 cut(s)	1064, 1190, 1233, 1509, 3291
BveI	ACCTGC	1 cut(s)	2158
Cac8I	GCNNGC	3 cut(s)	1896, 2192, 3008
CaiI	CAGNNNCTG	1 cut(s)	2831
CciI	TCATGA	1 cut(s)	2619
CfoI	GCGC	3 cut(s)	770, 1995, 3169
Cfr10I	RCCGGY	1 cut(s)	1800
Cfr13I	GGNCC	8 cut(s)	298, 665, 710, 935, 1020, 1728, 2087, 3350
Cfr9I	CCCGGG	1 cut(s)	1131
CseI	GACGC	3 cut(s)	871, 1170, 1467
Csp6I	GTAC	4 cut(s)	397, 930, 1145, 2335
CspAI	ACCGGT	1 cut(s)	1800
CviQI	GTAC	4 cut(s)	397, 930, 1145, 2335
DrdI	GACNNNNNNGTC	2 cut(s)	3156, 3280
DriI	GACNNNNNGTC	1 cut(s)	1026
DseDI	GACNNNNNNGTC	2 cut(s)	3156, 3280
EaeI	YGGCCR	1 cut(s)	2399
Eam1104I	CTCTTC	2 cut(s)	1670, 2827
Eam1105I	GACNNNNNGTC	1 cut(s)	1026
EarI	CTCTTC	2 cut(s)	1670, 2827
Eco105I	TACGTA	1 cut(s)	2563
Eco130I	CCWWGG	2 cut(s)	706, 857
Eco147I	AGGCCT	1 cut(s)	2787
Eco24I	GRGCYC	3 cut(s)	706, 1250, 2052
Eco31I	GGTCTC	3 cut(s)	2593, 2824, 3036
Eco47I	GGWCC	5 cut(s)	298, 665, 710, 1020, 3350
Eco47III	AGCGCT	1 cut(s)	769
Eco57I	CTGAAG	3 cut(s)	355, 2808, 3338
Eco88I	CYCGRG	1 cut(s)	1131
Eco91I	GGTNACC	1 cut(s)	2765
EcoNI	CCTNNNNNAGG	2 cut(s)	13, 1004
EcoO109I	RGGNCCY	2 cut(s)	298, 2087
EcoO65I	GGTNACC	1 cut(s)	2765
EcoRI	GAATTC	1 cut(s)	2478
EcoRII	CCWGG	5 cut(s)	135, 739, 811, 1615, 3404
EcoT14I	CCWWGG	2 cut(s)	706, 857
EcoT22I	ATGCAT	1 cut(s)	2425
EcoT38I	GRGCYC	3 cut(s)	706, 1250, 2052
ErhI	CCWWGG	2 cut(s)	706, 857
FalI	AAGNNNNNCTT	6 cut(s)	203, 235, 984, 1016, 3030, 3062
FaqI	GGGAC	3 cut(s)	164, 416, 1105
FauI	CCCGC	2 cut(s)	1880, 2573
FauNDI	CATATG	1 cut(s)	2443
FbaI	TGATCA	1 cut(s)	2953
FblI	GTMKAC	1 cut(s)	1528
FriOI	GRGCYC	3 cut(s)	706, 1250, 2052
FspBI	CTAG	9 cut(s)	83, 831, 1085, 1808, 1899, 1905, 2084, 2436, 3378
GlaI	GCGC	3 cut(s)	769, 1994, 3168
GsaI	CCCAGC	2 cut(s)	756, 941
GsuI	CTGGAG	1 cut(s)	1338
HaeII	RGCGCY	2 cut(s)	771, 3170
HaeIII	GGCC	7 cut(s)	140, 936, 1482, 1730, 2088, 2401, 2787
HapII	CCGG	5 cut(s)	141, 1132, 1250, 1455, 1801
HgaI	GACGC	3 cut(s)	871, 1170, 1467
HhaI	GCGC	3 cut(s)	770, 1995, 3169
Hin1I	GRCGYC	1 cut(s)	863
Hin6I	GCGC	3 cut(s)	768, 1993, 3167
HinP1I	GCGC	3 cut(s)	768, 1993, 3167
HindIII	AAGCTT	4 cut(s)	1569, 2283, 2387, 3008
HpaII	CCGG	5 cut(s)	141, 1132, 1250, 1455, 1801
HphI	GGTGA	5 cut(s)	779, 976, 1703, 1829, 2759
Hpy166II	GTNNAC	7 cut(s)	506, 1529, 1603, 1629, 2337, 2383, 3020
Hpy8I	GTNNAC	7 cut(s)	506, 1529, 1603, 1629, 2337, 2383, 3020
HpyCH4III	ACNGT	5 cut(s)	42, 73, 510, 2457, 3116
HpyCH4IV	ACGT	3 cut(s)	1353, 2131, 2562
HpyF10VI	GCNNNNNNNGC	8 cut(s)	382, 800, 1646, 1877, 2360, 2375, 2537, 3173
HpySE526I	ACGT	3 cut(s)	1353, 2131, 2562
Hsp92I	GRCGYC	1 cut(s)	863
HspAI	GCGC	3 cut(s)	768, 1993, 3167
Ksp22I	TGATCA	1 cut(s)	2953
LmnI	GCTCC	8 cut(s)	701, 811, 1245, 1495, 1618, 2122, 2828, 2853
MaeI	CTAG	9 cut(s)	83, 831, 1085, 1808, 1899, 1905, 2084, 2436, 3378
MaeII	ACGT	3 cut(s)	1353, 2131, 2562
MaeIII	GTNAC	5 cut(s)	874, 1408, 1942, 2765, 3110
MboII	GAAGA	8 cut(s)	97, 203, 208, 223, 1073, 1573, 1657, 2814
MfeI	CAATTG	1 cut(s)	1397
MflI	RGATCY	6 cut(s)	213, 629, 1034, 1127, 2214, 2266
MhlI	GDGCHC	6 cut(s)	706, 912, 1250, 1511, 1631, 2052
MlyI	GAGTC	8 cut(s)	101, 541, 1656, 1902, 2294, 2702, 2797, 3144
MmeI	TCCRAC	6 cut(s)	643, 1337, 1989, 2144, 2170, 2905
Mph1103I	ATGCAT	1 cut(s)	2425
MroXI	GAANNNNTTC	3 cut(s)	72, 215, 1802
MslI	CAYNNNNRTG	2 cut(s)	1634, 2458
MspA1I	CMGCKG	9 cut(s)	839, 899, 941, 1814, 1871, 1959, 2312, 2516, 3201
MspCI	CTTAAG	3 cut(s)	302, 2759, 3145
MspI	CCGG	5 cut(s)	141, 1132, 1250, 1455, 1801
MspR9I	CCNGG	8 cut(s)	137, 741, 813, 1132, 1133, 1250, 1617, 3406
MunI	CAATTG	1 cut(s)	1397
Mva1269I	GAATGC	2 cut(s)	1204, 2663
MvaI	CCWGG	5 cut(s)	137, 741, 813, 1617, 3406
MvnI	CGCG	2 cut(s)	1550, 2404
MwoI	GCNNNNNNNGC	8 cut(s)	382, 800, 1646, 1877, 2360, 2375, 2537, 3173
NciI	CCSGG	3 cut(s)	1132, 1133, 1250
NcoI	CCATGG	1 cut(s)	706
NdeI	CATATG	1 cut(s)	2443
NmuCI	GTSAC	2 cut(s)	2765, 3110
NsiI	ATGCAT	1 cut(s)	2425
NspI	RCATGY	2 cut(s)	2535, 3293
OliI	CACNNNNGTG	1 cut(s)	2458
PagI	TCATGA	1 cut(s)	2619
PceI	AGGCCT	1 cut(s)	2787
PctI	GAATGC	2 cut(s)	1204, 2663
PdmI	GAANNNNTTC	3 cut(s)	72, 215, 1802
PflFI	GACNNNGTC	1 cut(s)	155
PflMI	CCANNNNNTGG	2 cut(s)	680, 2924
PfoI	TCCNGGA	1 cut(s)	1615
PinAI	ACCGGT	1 cut(s)	1800
PleI	GAGTC	8 cut(s)	100, 540, 1656, 1902, 2294, 2702, 2797, 3144
PpsI	GAGTC	8 cut(s)	100, 540, 1656, 1902, 2294, 2702, 2797, 3144
Ppu21I	YACGTR	1 cut(s)	2563
PpuMI	RGGWCCY	1 cut(s)	298
PshAI	GACNNNNGTC	1 cut(s)	1110
Psp5II	RGGWCCY	1 cut(s)	298
Psp6I	CCWGG	5 cut(s)	135, 739, 811, 1615, 3404
PspEI	GGTNACC	1 cut(s)	2765
PspFI	CCCAGC	2 cut(s)	752, 937
PspGI	CCWGG	5 cut(s)	135, 739, 811, 1615, 3404
PspPI	GGNCC	8 cut(s)	298, 665, 710, 935, 1020, 1728, 2087, 3350
PspPPI	RGGWCCY	1 cut(s)	298
PstI	CTGCAG	2 cut(s)	1197, 1453
PstNI	CAGNNNCTG	1 cut(s)	2831
PsuI	RGATCY	6 cut(s)	213, 629, 1034, 1127, 2214, 2266
PsyI	GACNNNGTC	1 cut(s)	155
PvuII	CAGCTG	6 cut(s)	839, 899, 941, 1871, 2312, 2516
RsaI	GTAC	4 cut(s)	398, 931, 1146, 2336
RsaNI	GTAC	4 cut(s)	397, 930, 1145, 2335
RseI	CAYNNNNRTG	2 cut(s)	1634, 2458
Sau96I	GGNCC	8 cut(s)	298, 665, 710, 935, 1020, 1728, 2087, 3350
SchI	GAGTC	8 cut(s)	101, 541, 1656, 1902, 2294, 2702, 2797, 3144
ScrFI	CCNGG	8 cut(s)	137, 741, 813, 1132, 1133, 1250, 1617, 3406
SduI	GDGCHC	6 cut(s)	706, 912, 1250, 1511, 1631, 2052
SfcI	CTRYAG	2 cut(s)	1193, 1449
SinI	GGWCC	5 cut(s)	298, 665, 710, 1020, 3350
SmaI	CCCGGG	1 cut(s)	1133
SmiMI	CAYNNNNRTG	2 cut(s)	1634, 2458
SmlI	CTYRAG	6 cut(s)	10, 302, 2673, 2759, 3145, 3339
SmoI	CTYRAG	6 cut(s)	10, 302, 2673, 2759, 3145, 3339
SnaBI	TACGTA	1 cut(s)	2563
SseBI	AGGCCT	1 cut(s)	2787
SspI	AATATT	1 cut(s)	1078
SspMI	CTAG	9 cut(s)	83, 831, 1085, 1808, 1899, 1905, 2084, 2436, 3378
StuI	AGGCCT	1 cut(s)	2787
StyD4I	CCNGG	8 cut(s)	135, 739, 811, 1130, 1131, 1248, 1615, 3404
StyI	CCWWGG	2 cut(s)	706, 857
TaaI	ACNGT	5 cut(s)	42, 73, 510, 2457, 3116
TaiI	ACGT	3 cut(s)	1356, 2134, 2565
TaqI	TCGA	4 cut(s)	159, 255, 2127, 3160
TatI	WGTACW	1 cut(s)	2334
TauI	GCSGC	2 cut(s)	1814, 2404
TscAI	CASTG	5 cut(s)	1071, 1197, 1233, 1509, 3291
TseFI	GTSAC	2 cut(s)	2765, 3110
Tsp45I	GTSAC	2 cut(s)	2765, 3110
TspGWI	ACGGA	4 cut(s)	480, 1753, 2017, 2227
TspMI	CCCGGG	1 cut(s)	1131
TspRI	CASTG	5 cut(s)	1071, 1197, 1233, 1509, 3291
Tth111I	GACNNNGTC	1 cut(s)	155
Van91I	CCANNNNNTGG	2 cut(s)	680, 2924
Vha464I	CTTAAG	3 cut(s)	302, 2759, 3145
VneI	GTGCAC	1 cut(s)	1627
VpaK11BI	GGWCC	5 cut(s)	298, 665, 710, 1020, 3350
XagI	CCTNNNNNAGG	2 cut(s)	13, 1004
XapI	RAATTY	3 cut(s)	1823, 2250, 2478
XceI	RCATGY	2 cut(s)	2535, 3293
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3263
XmaI	CCCGGG	1 cut(s)	1131
XmiI	GTMKAC	1 cut(s)	1528
XmnI	GAANNNNTTC	3 cut(s)	72, 215, 1802
XspI	CTAG	9 cut(s)	83, 831, 1085, 1808, 1899, 1905, 2084, 2436, 3378
Zsp2I	ATGCAT	1 cut(s)	2425

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.