Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1B

Orthogroup ID

OG0002550

Physical Location & Seq

Genomic Coordinates Reverse (-)

53733898 .. 53744066

Gene Structure 10169 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1BG415700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1506 bp

ATGATAAAAGCCAGCCCGCCGCCGATCGAGGAGGGGTTCAACTTGCTGACAGAGAACGTGAAGAATGTGGAGAAGCTAGCCGGAAAAGGAGTGGAGCGTTGCTTGAAGCGGACGGGCTTCGAGTATAGCACTCTGCAGGCGCCAGAGTTGGGGACGGCAAGGTCGCGAGTGAGACGCACGCTGCCATTGTTCAAGTGGGCGTCGCTGAGGAGCTTGAGGCTGCTGAGGCTTGAAATTAACGCTGGTTTACGCTTGATAAGGATGTTTGAGGGCAAAGCTTTGAAGAGAAACCGAGGCACTACTGAGCCCATCAACACCAAGCATTTGATTGGCTATGCAGTTGTAGGCCCCATTGGGAATGTTTCTAACACTGTTGCTTCAACCCTGGAACGCGCTGATAGAATCCAACCTGCTGTTTCTGTCAGTAATGTGGGTAATATGGAGAACAGCGGGCTTGTTGATGTTAAACCATGGATCCCTGATGTTATAGACAAGATTAAGAGATGGAAAGTTTCTGATATTGCGGATCCTTCTCAAATGAAAGCTTTAAGATTGCCCGACTCTAAAACAGCTGGAAAGGTTGTGAGACTAATGCATATTTGGAAATGGCCGCGTGATGACAGGAATAATCCATCAGGAAAGGCTAGTGCATATGTTGTTCCACAGTTGTGGCAGCCTCGGAATGGAATTCTTATGTCTAATGATGTAAATGACAATAAGACTGCTGAGGAATATACTGCATGCATTGCTGTATATAAAAATGGTTCTTACATGATGTCTGCATCTGGCGGTAAGGTCTCTTTGTTCAACATGATGACATTTAAGGTCGAAACCAAACTGAAGGGTCACCGGAATCGGATAACGGGCCTTGCGTTTTCCCAAACTCTTAACATACTGGTTTCTTCAGGAGCTGATGCCCAGTTATGTGTGTGGAGCATTTTTGGGTGGGAGAAAAAGAAAACAAGGTTAATACAAGCACCAACTGGTCGGCAATCCCCATTGGTTGGAGAAACAAAAATTCAGTTTCATAACGATCACACACAATTGTTGGTTGCCCATGAAAGCCAAATTGCTGCGTATGACAGCAAGCTTGATTGTTTACGCTCTTGGACTCCCAAAGATGCACTTGCTGCTCCCATCTCATGTGCAATATATTCATGTGATGGTTTGTTGGTTTATGCTACTTTTTGTGACGGTGCTGTGGGAGTTTTTGAGGCTAATAGCTTAAGACTCAGATGTCGAATGGTGCCTTCTGCTTATATACCATCATTTTCTGTCAGCGGTGGCAACCCCTCCTATCCTCTGGTTGTAGCAGCTCATCCATCTGAGCCTAACCAGATTGCAGTTGGCATGACTGACGGGTCAGTGCATGTGGTTGAACCATCTGATGCAGAGCTGAAGTGGGGTGGCACACCCTCTCAAGATAATGGTCCTTCCAATTCATCAAATCCTTCTCCTAGTGGTCAAGCATCAGAACTTCCTTCCAGGTCAGGAAGAATGTGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003002 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009753 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009867 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009933 GO:0009987 GO:0010014 GO:0010016 GO:0010033 GO:0010051 GO:0010072 GO:0010154 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032870 GO:0042221 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045892 GO:0045934 GO:0046983 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048519 GO:0048523 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0080090 GO:0090421 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

501

Amino Acids

54.83

Weight (kDa)

9.5

Isoelectric Point (pI)

47.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR3_1st	PF25173	224 - 315	9.5e-11	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	241 - 314	5.5e-06	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	242 - 318	2.8e-06	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	246 - 408	1.6e-12	WDHD1 first WD40 domain
Beta-prop_WDR5	PF25175	247 - 409	3.8e-10	WDR5 beta-propeller domain
WD40_Prp19	PF24814	248 - 409	6.3e-09	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	255 - 410	2.5e-06	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002550)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770
malus_domestica	MD01G1234100.v1.1 MD07G1306300.v1.1
prunus_persica	Prupe.2G324300_v2.0.a1
pyrus_communis	pycom01g24260 pycom07g27930
rosa_chinensis	RchiOBHm_Chr1g0382471 RchiOBHm_Chr1g0382481 RchiOBHm_Chr1g0383911
rosa_laevigata	RLG00000026087 RLG00000026174 RLG00000026177
rosa_multiflora	Rmu_sc0000110.1_g000051 Rmu_sc0003885.1_g000009 Rmu_sc0005139.1_g000028
rosa_roxburghii	Rroxscaffold_4G00277160 Rroxscaffold_4G00278020 Rroxscaffold_4G00278080
rosa_rugosa	Rorug01G0434600 Rorug01G0434900 Rorug01G0443000
rosa_samantha	Rh1AG459200 Rh1AG459300 Rh1AG459600 Rh1AG468900 Rh1BG415700 Rh1BG426600 Rh1CG429700 Rh1CG429800 Rh1CG439400 Rh1DG447800 Rh1DG447900 Rh1DG459400
rosa_wichuraiana	Rw1G040350 Rw1G041180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	160, 1236, 1360
Acc36I	ACCTGC	1 cut(s)	418
AccB1I	GGYRCC	2 cut(s)	139, 1246
AccB7I	CCANNNNNTGG	1 cut(s)	1004
AccII	CGCG	3 cut(s)	166, 393, 613
AciI	CCGC	8 cut(s)	17, 20, 109, 450, 524, 611, 789, 1281
AclWI	GGATC	4 cut(s)	469, 482, 521, 534
AcoI	YGGCCR	1 cut(s)	608
AcsI	RAATTY	2 cut(s)	687, 1017
AcuI	CTGAAG	3 cut(s)	860, 888, 1418
AcyI	GRCGYC	2 cut(s)	140, 200
AfiI	CCNNNNNNNGG	3 cut(s)	149, 683, 1004
AflII	CTTAAG	1 cut(s)	1225
AgsI	TTSAA	8 cut(s)	40, 106, 193, 233, 283, 381, 808, 1379
AjnI	CCWGG	2 cut(s)	384, 1484
AleI	CACNNNNGTG	1 cut(s)	667
Alw26I	GTCTC	3 cut(s)	166, 580, 802
AlwI	GGATC	4 cut(s)	469, 482, 521, 534
AlwNI	CAGNNNCTG	1 cut(s)	911
AoxI	GGCC	3 cut(s)	346, 608, 865
ApeKI	GCWGC	6 cut(s)	181, 220, 673, 1073, 1130, 1313
ApoI	RAATTY	2 cut(s)	687, 1017
AspLEI	GCGC	2 cut(s)	142, 395
AspS9I	GGNCC	3 cut(s)	347, 865, 1430
AsuHPI	GGTGA	1 cut(s)	839
AsuNHI	GCTAGC	1 cut(s)	76
AvaII	GGWCC	1 cut(s)	1430
BamHI	GGATCC	2 cut(s)	474, 526
BanI	GGYRCC	2 cut(s)	139, 1246
BanII	GRGCYC	1 cut(s)	309
BbvCI	CCTCAGC	3 cut(s)	206, 224, 726
BbvI	GCAGC	6 cut(s)	168, 207, 685, 1060, 1117, 1325
BccI	CCATC	8 cut(s)	317, 498, 640, 1145, 1157, 1273, 1330, 1390
BceAI	ACGGC	1 cut(s)	171
BciT130I	CCWGG	2 cut(s)	386, 1486
BcoDI	GTCTC	3 cut(s)	166, 580, 802
BfaI	CTAG	3 cut(s)	77, 645, 1458
BfmI	CTRYAG	1 cut(s)	134
BfoI	RGCGCY	1 cut(s)	143
BfrI	CTTAAG	1 cut(s)	1225
BfuAI	ACCTGC	1 cut(s)	418
BisI	GCNGC	8 cut(s)	20, 182, 221, 611, 674, 1074, 1131, 1314
BlsI	GCNGC	8 cut(s)	21, 183, 222, 612, 675, 1075, 1132, 1315
Bme1390I	CCNGG	2 cut(s)	386, 1486
Bme18I	GGWCC	1 cut(s)	1430
BmgT120I	GGNCC	3 cut(s)	347, 865, 1430
BmiI	GGNNCC	5 cut(s)	141, 349, 476, 528, 1248
BmrFI	CCNGG	2 cut(s)	386, 1486
BmrI	ACTGGG	1 cut(s)	913
BmsI	GCATC	5 cut(s)	791, 904, 1111, 1378, 1478
BmtI	GCTAGC	1 cut(s)	80
BmuI	ACTGGG	1 cut(s)	913
Bpu10I	CCTNAGC	3 cut(s)	206, 224, 726
BpuEI	CTTGAG	2 cut(s)	235, 1404
BsaHI	GRCGYC	2 cut(s)	140, 200
BsaI	GGTCTC	1 cut(s)	802
BsaJI	CCNNGG	4 cut(s)	292, 384, 470, 677
BsaWI	WCCGGW	1 cut(s)	849
Bsc4I	CCNNNNNNNGG	3 cut(s)	149, 683, 1004
Bse1I	ACTGG	3 cut(s)	900, 919, 988
Bse3DI	GCAATG	1 cut(s)	744
BseBI	CCWGG	2 cut(s)	386, 1486
BseDI	CCNNGG	4 cut(s)	292, 384, 470, 677
BseGI	GGATG	2 cut(s)	267, 1318
BseLI	CCNNNNNNNGG	3 cut(s)	149, 683, 1004
BseMI	GCAATG	1 cut(s)	744
BseMII	CTCAG	6 cut(s)	197, 215, 294, 717, 1246, 1317
BseNI	ACTGG	3 cut(s)	900, 919, 988
BseRI	GAGGAG	2 cut(s)	44, 223
BseXI	GCAGC	6 cut(s)	168, 207, 685, 1060, 1117, 1325
Bsh1236I	CGCG	3 cut(s)	166, 393, 613
Bsh1285I	CGRYCG	1 cut(s)	27
BshFI	GGCC	3 cut(s)	348, 610, 867
BshNI	GGYRCC	2 cut(s)	139, 1246
BsiEI	CGRYCG	1 cut(s)	27
BsiSI	CCGG	2 cut(s)	81, 850
BslFI	GGGAC	1 cut(s)	166
BslI	CCNNNNNNNGG	3 cut(s)	149, 683, 1004
BsmAI	GTCTC	3 cut(s)	166, 580, 802
BsmBI	CGTCTC	1 cut(s)	166
BsmFI	GGGAC	1 cut(s)	166
BsnI	GGCC	3 cut(s)	348, 610, 867
Bso31I	GGTCTC	1 cut(s)	802
Bsp1286I	GDGCHC	1 cut(s)	309
Bsp143I	GATC	4 cut(s)	24, 474, 526, 1033
Bsp19I	CCATGG	1 cut(s)	470
Bsp68I	TCGCGA	1 cut(s)	166
BspACI	CCGC	8 cut(s)	17, 20, 109, 450, 524, 611, 789, 1281
BspANI	GGCC	3 cut(s)	348, 610, 867
BspCNI	CTCAG	6 cut(s)	198, 216, 295, 718, 1245, 1318
BspFNI	CGCG	3 cut(s)	166, 393, 613
BspLI	GGNNCC	5 cut(s)	141, 349, 476, 528, 1248
BspMAI	CTGCAG	1 cut(s)	138
BspMI	ACCTGC	1 cut(s)	418
BspOI	GCTAGC	1 cut(s)	80
BspPI	GGATC	4 cut(s)	469, 482, 521, 534
BspT107I	GGYRCC	2 cut(s)	139, 1246
BspTI	CTTAAG	1 cut(s)	1225
BspTNI	GGTCTC	1 cut(s)	802
BsrDI	GCAATG	1 cut(s)	744
BsrI	ACTGG	3 cut(s)	900, 919, 988
BssECI	CCNNGG	4 cut(s)	292, 384, 470, 677
BssMI	GATC	4 cut(s)	24, 474, 526, 1033
BssNI	GRCGYC	2 cut(s)	140, 200
BssT1I	CCWWGG	1 cut(s)	470
Bst2UI	CCWGG	2 cut(s)	386, 1486
Bst4CI	ACNGT	3 cut(s)	373, 666, 1196
Bst6I	CTCTTC	1 cut(s)	278
BstACI	GRCGYC	2 cut(s)	140, 200
BstAFI	CTTAAG	1 cut(s)	1225
BstAPI	GCANNNNNTGC	2 cut(s)	746, 1130
BstC8I	GCNNGC	8 cut(s)	13, 17, 78, 138, 179, 452, 742, 1088
BstDEI	CTNAG	6 cut(s)	206, 224, 303, 726, 1232, 1326
BstDSI	CCRYGG	1 cut(s)	470
BstEII	GGTNACC	1 cut(s)	845
BstF5I	GGATG	2 cut(s)	267, 1318
BstFNI	CGCG	3 cut(s)	166, 393, 613
BstH2I	RGCGCY	1 cut(s)	143
BstHHI	GCGC	2 cut(s)	142, 395
BstKTI	GATC	4 cut(s)	27, 477, 529, 1036
BstMAI	GTCTC	3 cut(s)	166, 580, 802
BstMBI	GATC	4 cut(s)	24, 474, 526, 1033
BstMCI	CGRYCG	1 cut(s)	27
BstMWI	GCNNNNNNNGC	3 cut(s)	226, 746, 1130
BstNI	CCWGG	2 cut(s)	386, 1486
BstNSI	RCATGY	2 cut(s)	744, 1373
BstPI	GGTNACC	1 cut(s)	845
BstSCI	CCNGG	2 cut(s)	384, 1484
BstSFI	CTRYAG	1 cut(s)	134
BstUI	CGCG	3 cut(s)	166, 393, 613
BstV1I	GCAGC	6 cut(s)	168, 207, 685, 1060, 1117, 1325
BstX2I	RGATCY	2 cut(s)	474, 526
BstXI	CCANNNNNNTGG	1 cut(s)	669
BstYI	RGATCY	2 cut(s)	474, 526
BsuRI	GGCC	3 cut(s)	348, 610, 867
BtgI	CCRYGG	1 cut(s)	470
BtsCI	GGATG	2 cut(s)	267, 1318
BtsIMutI	CAGTG	2 cut(s)	369, 1371
BtuMI	TCGCGA	1 cut(s)	166
BveI	ACCTGC	1 cut(s)	418
Cac8I	GCNNGC	8 cut(s)	13, 17, 78, 138, 179, 452, 742, 1088
CaiI	CAGNNNCTG	1 cut(s)	911
CfoI	GCGC	2 cut(s)	142, 395
Cfr13I	GGNCC	3 cut(s)	347, 865, 1430
CseI	GACGC	2 cut(s)	183, 189
CviAII	CATG	9 cut(s)	471, 741, 772, 811, 1058, 1143, 1158, 1351, 1370
DdeI	CTNAG	6 cut(s)	206, 224, 303, 726, 1232, 1326
DinI	GGCGCC	1 cut(s)	141
DpnI	GATC	4 cut(s)	26, 476, 528, 1035
DpnII	GATC	4 cut(s)	24, 474, 526, 1033
DrdI	GACNNNNNNGTC	3 cut(s)	160, 1236, 1360
DseDI	GACNNNNNNGTC	3 cut(s)	160, 1236, 1360
EaeI	YGGCCR	1 cut(s)	608
Eam1104I	CTCTTC	1 cut(s)	278
EarI	CTCTTC	1 cut(s)	278
Eco130I	CCWWGG	1 cut(s)	470
Eco24I	GRGCYC	1 cut(s)	309
Eco31I	GGTCTC	1 cut(s)	802
Eco47I	GGWCC	1 cut(s)	1430
Eco57I	CTGAAG	3 cut(s)	860, 888, 1418
Eco91I	GGTNACC	1 cut(s)	845
EcoO109I	RGGNCCY	1 cut(s)	347
EcoO65I	GGTNACC	1 cut(s)	845
EcoRI	GAATTC	1 cut(s)	687
EcoRII	CCWGG	2 cut(s)	384, 1484
EcoT14I	CCWWGG	1 cut(s)	470
EcoT22I	ATGCAT	2 cut(s)	597, 746
EcoT38I	GRGCYC	1 cut(s)	309
EgeI	GGCGCC	1 cut(s)	141
EheI	GGCGCC	1 cut(s)	141
ErhI	CCWWGG	1 cut(s)	470
Esp3I	CGTCTC	1 cut(s)	166
FaeI	CATG	9 cut(s)	474, 744, 775, 814, 1061, 1146, 1161, 1354, 1373
FalI	AAGNNNNNCTT	2 cut(s)	1110, 1142
FaqI	GGGAC	1 cut(s)	166
FatI	CATG	9 cut(s)	470, 740, 771, 810, 1057, 1142, 1157, 1350, 1369
FauI	CCCGC	2 cut(s)	24, 443
FauNDI	CATATG	1 cut(s)	652
Fnu4HI	GCNGC	8 cut(s)	20, 182, 221, 611, 674, 1074, 1131, 1314
FokI	GGATG	2 cut(s)	274, 1305
FriOI	GRGCYC	1 cut(s)	309
Fsp4HI	GCNGC	8 cut(s)	20, 182, 221, 611, 674, 1074, 1131, 1314
FspBI	CTAG	3 cut(s)	77, 645, 1458
GlaI	GCGC	2 cut(s)	141, 394
GluI	GCNGC	8 cut(s)	20, 182, 221, 611, 674, 1074, 1131, 1314
HaeII	RGCGCY	1 cut(s)	143
HaeIII	GGCC	3 cut(s)	348, 610, 867
HapII	CCGG	2 cut(s)	81, 850
HgaI	GACGC	2 cut(s)	183, 189
HhaI	GCGC	2 cut(s)	142, 395
Hin1I	GRCGYC	2 cut(s)	140, 200
Hin1II	CATG	9 cut(s)	474, 744, 775, 814, 1061, 1146, 1161, 1354, 1373
Hin6I	GCGC	2 cut(s)	140, 393
HinP1I	GCGC	2 cut(s)	140, 393
HindIII	AAGCTT	3 cut(s)	276, 543, 1088
HinfI	GANTC	5 cut(s)	402, 560, 853, 1111, 1230
HpaII	CCGG	2 cut(s)	81, 850
HphI	GGTGA	1 cut(s)	839
Hpy166II	GTNNAC	2 cut(s)	248, 1100
Hpy188I	TCNGA	7 cut(s)	517, 681, 858, 1235, 1327, 1387, 1474
Hpy188III	TCNNGA	5 cut(s)	165, 636, 906, 1421, 1491
Hpy8I	GTNNAC	2 cut(s)	248, 1100
Hpy99I	CGWCG	1 cut(s)	205
HpyAV	CCTTC	6 cut(s)	540, 835, 1260, 1443, 1461, 1491
HpyCH4III	ACNGT	3 cut(s)	373, 666, 1196
HpyCH4IV	ACGT	1 cut(s)	57
HpyF10VI	GCNNNNNNNGC	3 cut(s)	226, 746, 1130
HpyF3I	CTNAG	6 cut(s)	206, 224, 303, 726, 1232, 1326
HpySE526I	ACGT	1 cut(s)	57
Hsp92I	GRCGYC	2 cut(s)	140, 200
Hsp92II	CATG	9 cut(s)	474, 744, 775, 814, 1061, 1146, 1161, 1354, 1373
HspAI	GCGC	2 cut(s)	140, 393
KasI	GGCGCC	1 cut(s)	139
Kzo9I	GATC	4 cut(s)	24, 474, 526, 1033
LmnI	GCTCC	5 cut(s)	94, 210, 908, 933, 1138
Lsp1109I	GCAGC	6 cut(s)	168, 207, 685, 1060, 1117, 1325
LweI	GCATC	5 cut(s)	791, 904, 1111, 1378, 1478
MaeI	CTAG	3 cut(s)	77, 645, 1458
MaeII	ACGT	1 cut(s)	57
MaeIII	GTNAC	2 cut(s)	845, 1190
MalI	GATC	4 cut(s)	26, 476, 528, 1035
MboI	GATC	4 cut(s)	24, 474, 526, 1033
MboII	GAAGA	4 cut(s)	73, 295, 894, 1506
MfeI	CAATTG	1 cut(s)	1043
MflI	RGATCY	2 cut(s)	474, 526
MhlI	GDGCHC	1 cut(s)	309
MluCI	AATT	6 cut(s)	234, 687, 1017, 1043, 1068, 1438
Mly113I	GGCGCC	1 cut(s)	140
MlyI	GAGTC	3 cut(s)	554, 1105, 1224
MmeI	TCCRAC	2 cut(s)	430, 985
Mph1103I	ATGCAT	2 cut(s)	597, 746
MseI	TTAA	9 cut(s)	237, 465, 498, 548, 822, 888, 968, 1226, 1504
MslI	CAYNNNNRTG	1 cut(s)	667
MspA1I	CMGCKG	3 cut(s)	450, 572, 1281
MspCI	CTTAAG	1 cut(s)	1225
MspI	CCGG	2 cut(s)	81, 850
MspR9I	CCNGG	2 cut(s)	386, 1486
MunI	CAATTG	1 cut(s)	1043
MvaI	CCWGG	2 cut(s)	386, 1486
MvnI	CGCG	3 cut(s)	166, 393, 613
MwoI	GCNNNNNNNGC	3 cut(s)	226, 746, 1130
NarI	GGCGCC	1 cut(s)	140
NcoI	CCATGG	1 cut(s)	470
NdeI	CATATG	1 cut(s)	652
NdeII	GATC	4 cut(s)	24, 474, 526, 1033
NheI	GCTAGC	1 cut(s)	76
NlaIII	CATG	9 cut(s)	474, 744, 775, 814, 1061, 1146, 1161, 1354, 1373
NlaIV	GGNNCC	5 cut(s)	141, 349, 476, 528, 1248
NmuCI	GTSAC	2 cut(s)	845, 1190
NruI	TCGCGA	1 cut(s)	166
NsiI	ATGCAT	2 cut(s)	597, 746
NspI	RCATGY	2 cut(s)	744, 1373
OliI	CACNNNNGTG	1 cut(s)	667
PaeI	GCATGC	1 cut(s)	744
PcsI	WCGNNNNNNNCGW	1 cut(s)	869
PfeI	GAWTC	2 cut(s)	402, 853
PflMI	CCANNNNNTGG	1 cut(s)	1004
PkrI	GCNGC	8 cut(s)	21, 183, 222, 612, 675, 1075, 1132, 1315
Ple19I	CGATCG	1 cut(s)	27
PleI	GAGTC	3 cut(s)	554, 1105, 1224
PluTI	GGCGCC	1 cut(s)	143
PpsI	GAGTC	3 cut(s)	554, 1105, 1224
Psp6I	CCWGG	2 cut(s)	384, 1484
PspEI	GGTNACC	1 cut(s)	845
PspGI	CCWGG	2 cut(s)	384, 1484
PspN4I	GGNNCC	5 cut(s)	141, 349, 476, 528, 1248
PspPI	GGNCC	3 cut(s)	347, 865, 1430
PstI	CTGCAG	1 cut(s)	138
PstNI	CAGNNNCTG	1 cut(s)	911
PsuI	RGATCY	2 cut(s)	474, 526
PvuI	CGATCG	1 cut(s)	27
PvuII	CAGCTG	1 cut(s)	572
RruI	TCGCGA	1 cut(s)	166
RseI	CAYNNNNRTG	1 cut(s)	667
SaqAI	TTAA	9 cut(s)	237, 465, 498, 548, 822, 888, 968, 1226, 1504
SatI	GCNGC	8 cut(s)	20, 182, 221, 611, 674, 1074, 1131, 1314
Sau3AI	GATC	4 cut(s)	24, 474, 526, 1033
Sau96I	GGNCC	3 cut(s)	347, 865, 1430
SchI	GAGTC	3 cut(s)	554, 1105, 1224
ScrFI	CCNGG	2 cut(s)	386, 1486
SduI	GDGCHC	1 cut(s)	309
SfaNI	GCATC	5 cut(s)	791, 904, 1111, 1378, 1478
SfcI	CTRYAG	1 cut(s)	134
SfoI	GGCGCC	1 cut(s)	141
SinI	GGWCC	1 cut(s)	1430
SmiMI	CAYNNNNRTG	1 cut(s)	667
SmlI	CTYRAG	3 cut(s)	214, 1225, 1419
SmoI	CTYRAG	3 cut(s)	214, 1225, 1419
SphI	GCATGC	1 cut(s)	744
Sse9I	AATT	6 cut(s)	234, 687, 1017, 1043, 1068, 1438
SsiI	CCGC	8 cut(s)	17, 20, 109, 450, 524, 611, 789, 1281
SspDI	GGCGCC	1 cut(s)	139
SspMI	CTAG	3 cut(s)	77, 645, 1458
StyD4I	CCNGG	2 cut(s)	384, 1484
StyI	CCWWGG	1 cut(s)	470
TaaI	ACNGT	3 cut(s)	373, 666, 1196
TaiI	ACGT	1 cut(s)	60
TaqI	TCGA	4 cut(s)	27, 120, 828, 1240
TasI	AATT	6 cut(s)	234, 687, 1017, 1043, 1068, 1438
TauI	GCSGC	2 cut(s)	22, 613
TfiI	GAWTC	2 cut(s)	402, 853
Tru1I	TTAA	9 cut(s)	237, 465, 498, 548, 822, 888, 968, 1226, 1504
Tru9I	TTAA	9 cut(s)	237, 465, 498, 548, 822, 888, 968, 1226, 1504
TscAI	CASTG	2 cut(s)	376, 1371
TseFI	GTSAC	2 cut(s)	845, 1190
TseI	GCWGC	6 cut(s)	181, 220, 673, 1073, 1130, 1313
Tsp45I	GTSAC	2 cut(s)	845, 1190
TspDTI	ATGAA	5 cut(s)	554, 1016, 1074, 1146, 1431
TspRI	CASTG	2 cut(s)	376, 1371
Van91I	CCANNNNNTGG	1 cut(s)	1004
Vha464I	CTTAAG	1 cut(s)	1225
VpaK11BI	GGWCC	1 cut(s)	1430
XapI	RAATTY	2 cut(s)	687, 1017
XceI	RCATGY	2 cut(s)	744, 1373
XcmI	CCANNNNNNNNNTGG	2 cut(s)	192, 1343
XspI	CTAG	3 cut(s)	77, 645, 1458
Zsp2I	ATGCAT	2 cut(s)	597, 746

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.