Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0002550

Physical Location & Seq

Genomic Coordinates Forward (+)

1220336 .. 1224051

Gene Structure 3716 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00278080.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1452 bp

ATGTTTGAGGGCAAAGCTTTGAAGAGAAACCGAGGCACTACTGAGCCCATCAACACCAAGGTAGTCTTTGCTACGAATACGATGCATGCCAATTGTCAGCCTTTGATTGGCTATGCAGTTGTAGGCCCCGTTGGGAATGTTTCTAACACTGTTGCTCCAACCCTGGAACGTGCTGATAGAATCCAACCTGCTGTTTCTGTCAGTAGTCTGGGTAATATGGAGAACAGCAGGCTTGTTGATGTTAAACCATGGATCCCTGATGTTATAGACATGACTAAGAGATGGAAAATTTTTGATATTGCGGATCCTTCTCAAATGAAAGCTTTAAGATTGCCTGACTCTAAAACAGCTGGAAAGGTTGTAAGACTAATGTACACCAATGATGGTTTGGCTCTGTTGGCCCTCGCCTCCAATGCTGTTCACAAGCTTTGGAAATGGCCGCGTAATGATAGGAATAATCCATCAGGGAAGGCAAGTGCATATGTTCTTCCACAGGTGTGGCAGCCTCGGAATGGAATTCTTATGGCTAATGATGTAAACGACAATAAACCAGCTGAGGAATCTACTGCATGCATTGCTTTGTCTAAAAATGATTCTTACATAATGTCTGCATCTGGCGGGAAGATCTCCTTTTTCAATATGATGACATTTAAGGTCATGAAAACATTTGTGTCTCCTCCTCCTGCTGCCACTTTTCTGGCATTCCATCCTCAAAATAATAATATAATTGCTATTGGCATGGAGGACTCTACTATTTTAATATATAATATCAGGGTTGATGAGGTCGAAACCAAACTGAAGGGTCACCGGAATCAGATAACGGGCCTTGCGTTTTCCCAAACTCTTAACATACTGGTTTCTTCAGGAGCTGATGCCCAGTTATGTGTGTGGAGCATTTTTGGGTGGGAGAAAAAGAAAACAAGGTTAATACAAGCACCAACTGGTCGGCAATCCCCATTGGTTGGAGAAACAAAAATTATTCAGTTTCATAACGATCACACACAATTGTTGGTTGCCCATGAAAGCCAAATTGCTGTGTATGACAACAAGCTTGATTGTTTACGCTCTTGGACTCCCAAAGATGCACTGGCTGCTCCCATCTCATGTGCAATATATTCATGCGATGGTTTGTTGGTATATGCTACTTTTTGTGACGGTGCTGTGGGAGTTTTTGATGCTAATAGCTTAAGACTCAGATGTCGAATGGTGCCTTCTGCTTATATACCATCATTTCCTCTCAGCGGTGGCAACCCCTCCTATCCTCTGGTTGTAGCAGCTCATCCATCTGAGCCTAACCAGATCGCAGTTGGCATGACTGACGGGTCAGTGCATGTGGTTGAACCATCTGATGCAGAGCTGAAGTGGGGTGGCACACCCTCTCAAGATAATGGTCCTTCCAATTCATCAAATCCTTCTCCTAGTGGTCAAGCATCAGAACTTCCTTCCAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003002 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009753 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009867 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009933 GO:0009987 GO:0010014 GO:0010016 GO:0010033 GO:0010051 GO:0010072 GO:0010154 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032870 GO:0042221 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045892 GO:0045934 GO:0046983 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048519 GO:0048523 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0080090 GO:0090421 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

483

Amino Acids

52.52

Weight (kDa)

8.65

Isoelectric Point (pI)

45.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR3_1st	PF25173	166 - 299	9.5e-17	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	180 - 300	4.5e-14	CDC20/Fizzy WD40 domain
WD40_WDHD1_1st	PF24817	183 - 395	3.3e-18	WDHD1 first WD40 domain
WDR55	PF24796	184 - 299	7.1e-07	WDR55
WD40_Prp19	PF24814	185 - 350	1.6e-12	Prp19 WD40 domain
Beta-prop_EML_2	PF23414	185 - 302	1.5e-09	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5	PF25175	188 - 430	6.7e-16	WDR5 beta-propeller domain
Beta-prop_HPS5	PF23756	190 - 349	2.5e-06	HPS5 beta-propeller
Beta-prop_WDR3_2nd	PF25172	191 - 308	5.5e-12	WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	192 - 299	2.6e-11	WDR36/Utp21 second beta-propeller domain
Beta-prop_IFT140_1st	PF23383	202 - 318	5.8e-11	IFT140 first beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	204 - 298	7.9e-10	WDR36/Utp21 first beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	208 - 314	1.3e-09	WDR90/POC16, second beta-propeller
Beta-prop_RIG_1st	PF23770	216 - 281	4.9e-07	RIG first beta-propeller
WD40_Prp19	PF24814	227 - 396	1.8e-13	Prp19 WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002550)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770
malus_domestica	MD01G1234100.v1.1 MD07G1306300.v1.1
prunus_persica	Prupe.2G324300_v2.0.a1
pyrus_communis	pycom01g24260 pycom07g27930
rosa_chinensis	RchiOBHm_Chr1g0382471 RchiOBHm_Chr1g0382481 RchiOBHm_Chr1g0383911
rosa_laevigata	RLG00000026087 RLG00000026174 RLG00000026177
rosa_multiflora	Rmu_sc0000110.1_g000051 Rmu_sc0003885.1_g000009 Rmu_sc0005139.1_g000028
rosa_roxburghii	Rroxscaffold_4G00277160 Rroxscaffold_4G00278020 Rroxscaffold_4G00278080
rosa_rugosa	Rorug01G0434600 Rorug01G0434900 Rorug01G0443000
rosa_samantha	Rh1AG459200 Rh1AG459300 Rh1AG459600 Rh1AG468900 Rh1BG415700 Rh1BG426600 Rh1CG429700 Rh1CG429800 Rh1CG439400 Rh1DG447800 Rh1DG447900 Rh1DG459400
rosa_wichuraiana	Rw1G040350 Rw1G041180

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	1197, 1321
Acc36I	ACCTGC	1 cut(s)	196
AccB1I	GGYRCC	1 cut(s)	1207
AccB7I	CCANNNNNTGG	1 cut(s)	962
AccII	CGCG	1 cut(s)	442
AciI	CCGC	4 cut(s)	302, 440, 618, 1242
AclWI	GGATC	4 cut(s)	247, 260, 299, 312
AcoI	YGGCCR	1 cut(s)	437
AcsI	RAATTY	2 cut(s)	288, 516
AcuI	CTGAAG	3 cut(s)	818, 846, 1379
AfaI	GTAC	1 cut(s)	374
AfiI	CCNNNNNNNGG	4 cut(s)	107, 512, 962, 1241
AflII	CTTAAG	1 cut(s)	1186
AgsI	TTSAA	3 cut(s)	22, 637, 1340
AjnI	CCWGG	2 cut(s)	162, 1445
AleI	CACNNNNGTG	1 cut(s)	496
Alw26I	GTCTC	1 cut(s)	678
AlwI	GGATC	4 cut(s)	247, 260, 299, 312
AlwNI	CAGNNNCTG	1 cut(s)	869
AoxI	GGCC	4 cut(s)	124, 399, 437, 823
ApeKI	GCWGC	4 cut(s)	502, 686, 1091, 1274
ApoI	RAATTY	2 cut(s)	288, 516
AspS9I	GGNCC	4 cut(s)	125, 400, 823, 1391
AsuHPI	GGTGA	1 cut(s)	797
AvaII	GGWCC	1 cut(s)	1391
BamHI	GGATCC	2 cut(s)	252, 304
BanI	GGYRCC	1 cut(s)	1207
BanII	GRGCYC	1 cut(s)	48
BbvCI	CCTCAGC	1 cut(s)	555
BbvI	GCAGC	4 cut(s)	514, 673, 1078, 1286
BcgI	CGANNNNNNTGC	2 cut(s)	64, 98
BciT130I	CCWGG	2 cut(s)	164, 1447
BcoDI	GTCTC	1 cut(s)	678
BfaI	CTAG	1 cut(s)	1419
BfrI	CTTAAG	1 cut(s)	1186
BfuAI	ACCTGC	1 cut(s)	196
BglII	AGATCT	1 cut(s)	624
BisI	GCNGC	5 cut(s)	440, 503, 687, 1092, 1275
BlsI	GCNGC	5 cut(s)	441, 504, 688, 1093, 1276
Bme1390I	CCNGG	2 cut(s)	164, 1447
Bme18I	GGWCC	1 cut(s)	1391
BmgT120I	GGNCC	4 cut(s)	125, 400, 823, 1391
BmiI	GGNNCC	4 cut(s)	127, 254, 306, 1209
BmrFI	CCNGG	2 cut(s)	164, 1447
BmrI	ACTGGG	1 cut(s)	871
BmsI	GCATC	7 cut(s)	72, 620, 862, 1072, 1165, 1339, 1439
BmuI	ACTGGG	1 cut(s)	871
Bpu10I	CCTNAGC	1 cut(s)	555
BpuEI	CTTGAG	1 cut(s)	1365
BsaJI	CCNNGG	5 cut(s)	31, 57, 162, 248, 506
BsaWI	WCCGGW	1 cut(s)	807
BsaXI	ACNNNNNCTCC	2 cut(s)	139, 169
Bsc4I	CCNNNNNNNGG	4 cut(s)	107, 512, 962, 1241
Bse1I	ACTGG	4 cut(s)	858, 877, 946, 1092
Bse3DI	GCAATG	1 cut(s)	573
BseBI	CCWGG	2 cut(s)	164, 1447
BseDI	CCNNGG	5 cut(s)	31, 57, 162, 248, 506
BseGI	GGATG	2 cut(s)	706, 1279
BseLI	CCNNNNNNNGG	4 cut(s)	107, 512, 962, 1241
BseMI	GCAATG	1 cut(s)	573
BseMII	CTCAG	5 cut(s)	33, 546, 1207, 1252, 1278
BseNI	ACTGG	4 cut(s)	858, 877, 946, 1092
BseRI	GAGGAG	2 cut(s)	666, 669
BseXI	GCAGC	4 cut(s)	514, 673, 1078, 1286
Bsh1236I	CGCG	1 cut(s)	442
BshFI	GGCC	4 cut(s)	126, 401, 439, 825
BshNI	GGYRCC	1 cut(s)	1207
BsiSI	CCGG	1 cut(s)	808
BslI	CCNNNNNNNGG	4 cut(s)	107, 512, 962, 1241
BsmAI	GTCTC	1 cut(s)	678
BsmI	GAATGC	1 cut(s)	701
BsnI	GGCC	4 cut(s)	126, 401, 439, 825
Bsp1286I	GDGCHC	1 cut(s)	48
Bsp1407I	TGTACA	1 cut(s)	372
Bsp143I	GATC	5 cut(s)	252, 304, 624, 994, 1299
Bsp19I	CCATGG	1 cut(s)	248
BspACI	CCGC	4 cut(s)	302, 440, 618, 1242
BspANI	GGCC	4 cut(s)	126, 401, 439, 825
BspCNI	CTCAG	5 cut(s)	34, 547, 1206, 1251, 1279
BspFNI	CGCG	1 cut(s)	442
BspHI	TCATGA	1 cut(s)	657
BspLI	GGNNCC	4 cut(s)	127, 254, 306, 1209
BspMI	ACCTGC	1 cut(s)	196
BspPI	GGATC	4 cut(s)	247, 260, 299, 312
BspT107I	GGYRCC	1 cut(s)	1207
BspTI	CTTAAG	1 cut(s)	1186
BsrDI	GCAATG	1 cut(s)	573
BsrGI	TGTACA	1 cut(s)	372
BsrI	ACTGG	4 cut(s)	858, 877, 946, 1092
BssECI	CCNNGG	5 cut(s)	31, 57, 162, 248, 506
BssMI	GATC	5 cut(s)	252, 304, 624, 994, 1299
BssT1I	CCWWGG	2 cut(s)	57, 248
Bst2UI	CCWGG	2 cut(s)	164, 1447
Bst4CI	ACNGT	2 cut(s)	151, 1157
Bst6I	CTCTTC	1 cut(s)	17
BstAFI	CTTAAG	1 cut(s)	1186
BstAPI	GCANNNNNTGC	2 cut(s)	575, 1091
BstAUI	TGTACA	1 cut(s)	372
BstC8I	GCNNGC	3 cut(s)	87, 230, 571
BstDEI	CTNAG	6 cut(s)	42, 276, 555, 1193, 1238, 1287
BstDSI	CCRYGG	1 cut(s)	248
BstEII	GGTNACC	1 cut(s)	803
BstF5I	GGATG	2 cut(s)	706, 1279
BstFNI	CGCG	1 cut(s)	442
BstKTI	GATC	5 cut(s)	255, 307, 627, 997, 1302
BstMAI	GTCTC	1 cut(s)	678
BstMBI	GATC	5 cut(s)	252, 304, 624, 994, 1299
BstMWI	GCNNNNNNNGC	4 cut(s)	398, 413, 575, 1091
BstNI	CCWGG	2 cut(s)	164, 1447
BstNSI	RCATGY	3 cut(s)	89, 573, 1334
BstPI	GGTNACC	1 cut(s)	803
BstSCI	CCNGG	2 cut(s)	162, 1445
BstUI	CGCG	1 cut(s)	442
BstV1I	GCAGC	4 cut(s)	514, 673, 1078, 1286
BstX2I	RGATCY	3 cut(s)	252, 304, 624
BstXI	CCANNNNNNTGG	2 cut(s)	498, 697
BstYI	RGATCY	3 cut(s)	252, 304, 624
BsuRI	GGCC	4 cut(s)	126, 401, 439, 825
BtgI	CCRYGG	1 cut(s)	248
BtgZI	GCGATG	1 cut(s)	1137
BtsCI	GGATG	2 cut(s)	706, 1279
BtsIMutI	CAGTG	3 cut(s)	147, 1085, 1332
BveI	ACCTGC	1 cut(s)	196
Cac8I	GCNNGC	3 cut(s)	87, 230, 571
CaiI	CAGNNNCTG	1 cut(s)	869
CciI	TCATGA	1 cut(s)	657
Cfr13I	GGNCC	4 cut(s)	125, 400, 823, 1391
Csp6I	GTAC	1 cut(s)	373
CviQI	GTAC	1 cut(s)	373
DdeI	CTNAG	6 cut(s)	42, 276, 555, 1193, 1238, 1287
DpnI	GATC	5 cut(s)	254, 306, 626, 996, 1301
DpnII	GATC	5 cut(s)	252, 304, 624, 994, 1299
DrdI	GACNNNNNNGTC	2 cut(s)	1197, 1321
DseDI	GACNNNNNNGTC	2 cut(s)	1197, 1321
EaeI	YGGCCR	1 cut(s)	437
Eam1104I	CTCTTC	1 cut(s)	17
EarI	CTCTTC	1 cut(s)	17
Eco130I	CCWWGG	2 cut(s)	57, 248
Eco24I	GRGCYC	1 cut(s)	48
Eco47I	GGWCC	1 cut(s)	1391
Eco57I	CTGAAG	3 cut(s)	818, 846, 1379
Eco91I	GGTNACC	1 cut(s)	803
EcoO109I	RGGNCCY	1 cut(s)	125
EcoO65I	GGTNACC	1 cut(s)	803
EcoRI	GAATTC	1 cut(s)	516
EcoRII	CCWGG	2 cut(s)	162, 1445
EcoT14I	CCWWGG	2 cut(s)	57, 248
EcoT22I	ATGCAT	2 cut(s)	87, 575
EcoT38I	GRGCYC	1 cut(s)	48
ErhI	CCWWGG	2 cut(s)	57, 248
FalI	AAGNNNNNCTT	4 cut(s)	50, 82, 614, 646
FauI	CCCGC	1 cut(s)	611
FauNDI	CATATG	1 cut(s)	481
Fnu4HI	GCNGC	5 cut(s)	440, 503, 687, 1092, 1275
FokI	GGATG	2 cut(s)	693, 1266
FriOI	GRGCYC	1 cut(s)	48
Fsp4HI	GCNGC	5 cut(s)	440, 503, 687, 1092, 1275
FspBI	CTAG	1 cut(s)	1419
GluI	GCNGC	5 cut(s)	440, 503, 687, 1092, 1275
HaeIII	GGCC	4 cut(s)	126, 401, 439, 825
HapII	CCGG	1 cut(s)	808
HindIII	AAGCTT	4 cut(s)	15, 321, 425, 1049
HinfI	GANTC	8 cut(s)	180, 338, 560, 593, 746, 811, 1072, 1191
HpaII	CCGG	1 cut(s)	808
HphI	GGTGA	1 cut(s)	797
Hpy166II	GTNNAC	4 cut(s)	375, 421, 538, 1061
Hpy188I	TCNGA	6 cut(s)	510, 816, 1196, 1288, 1348, 1435
Hpy188III	TCNNGA	3 cut(s)	658, 864, 1382
Hpy8I	GTNNAC	4 cut(s)	375, 421, 538, 1061
HpyAV	CCTTC	7 cut(s)	318, 463, 793, 1221, 1404, 1422, 1452
HpyCH4III	ACNGT	2 cut(s)	151, 1157
HpyCH4IV	ACGT	1 cut(s)	169
HpyF10VI	GCNNNNNNNGC	4 cut(s)	398, 413, 575, 1091
HpyF3I	CTNAG	6 cut(s)	42, 276, 555, 1193, 1238, 1287
HpySE526I	ACGT	1 cut(s)	169
Kzo9I	GATC	5 cut(s)	252, 304, 624, 994, 1299
LmnI	GCTCC	4 cut(s)	160, 866, 891, 1099
Lsp1109I	GCAGC	4 cut(s)	514, 673, 1078, 1286
LweI	GCATC	7 cut(s)	72, 620, 862, 1072, 1165, 1339, 1439
MaeI	CTAG	1 cut(s)	1419
MaeII	ACGT	1 cut(s)	169
MaeIII	GTNAC	2 cut(s)	803, 1151
MalI	GATC	5 cut(s)	254, 306, 626, 996, 1301
MboI	GATC	5 cut(s)	252, 304, 624, 994, 1299
MboII	GAAGA	4 cut(s)	34, 479, 634, 852
MfeI	CAATTG	2 cut(s)	91, 1004
MflI	RGATCY	3 cut(s)	252, 304, 624
MhlI	GDGCHC	1 cut(s)	48
MluCI	AATT	8 cut(s)	91, 288, 516, 726, 975, 1004, 1029, 1399
MlyI	GAGTC	4 cut(s)	332, 740, 1066, 1185
MmeI	TCCRAC	3 cut(s)	182, 208, 943
Mph1103I	ATGCAT	2 cut(s)	87, 575
MseI	TTAA	7 cut(s)	243, 326, 651, 758, 846, 926, 1187
MslI	CAYNNNNRTG	1 cut(s)	496
MspA1I	CMGCKG	3 cut(s)	350, 554, 1242
MspCI	CTTAAG	1 cut(s)	1186
MspI	CCGG	1 cut(s)	808
MspR9I	CCNGG	2 cut(s)	164, 1447
MunI	CAATTG	2 cut(s)	91, 1004
Mva1269I	GAATGC	1 cut(s)	701
MvaI	CCWGG	2 cut(s)	164, 1447
MvnI	CGCG	1 cut(s)	442
MwoI	GCNNNNNNNGC	4 cut(s)	398, 413, 575, 1091
NcoI	CCATGG	1 cut(s)	248
NdeI	CATATG	1 cut(s)	481
NdeII	GATC	5 cut(s)	252, 304, 624, 994, 1299
NlaIV	GGNNCC	4 cut(s)	127, 254, 306, 1209
NmuCI	GTSAC	2 cut(s)	803, 1151
NsiI	ATGCAT	2 cut(s)	87, 575
NspI	RCATGY	3 cut(s)	89, 573, 1334
OliI	CACNNNNGTG	1 cut(s)	496
PaeI	GCATGC	2 cut(s)	89, 573
PagI	TCATGA	1 cut(s)	657
PcsI	WCGNNNNNNNCGW	1 cut(s)	827
PctI	GAATGC	1 cut(s)	701
PfeI	GAWTC	4 cut(s)	180, 560, 593, 811
PflMI	CCANNNNNTGG	1 cut(s)	962
PkrI	GCNGC	5 cut(s)	441, 504, 688, 1093, 1276
PleI	GAGTC	4 cut(s)	332, 740, 1066, 1185
PpsI	GAGTC	4 cut(s)	332, 740, 1066, 1185
Psp6I	CCWGG	2 cut(s)	162, 1445
PspEI	GGTNACC	1 cut(s)	803
PspGI	CCWGG	2 cut(s)	162, 1445
PspN4I	GGNNCC	4 cut(s)	127, 254, 306, 1209
PspPI	GGNCC	4 cut(s)	125, 400, 823, 1391
PstNI	CAGNNNCTG	1 cut(s)	869
PsuI	RGATCY	3 cut(s)	252, 304, 624
PvuII	CAGCTG	2 cut(s)	350, 554
RsaI	GTAC	1 cut(s)	374
RsaNI	GTAC	1 cut(s)	373
RseI	CAYNNNNRTG	1 cut(s)	496
SaqAI	TTAA	7 cut(s)	243, 326, 651, 758, 846, 926, 1187
SatI	GCNGC	5 cut(s)	440, 503, 687, 1092, 1275
Sau3AI	GATC	5 cut(s)	252, 304, 624, 994, 1299
Sau96I	GGNCC	4 cut(s)	125, 400, 823, 1391
SchI	GAGTC	4 cut(s)	332, 740, 1066, 1185
ScrFI	CCNGG	2 cut(s)	164, 1447
SduI	GDGCHC	1 cut(s)	48
SfaNI	GCATC	7 cut(s)	72, 620, 862, 1072, 1165, 1339, 1439
SinI	GGWCC	1 cut(s)	1391
SmiMI	CAYNNNNRTG	1 cut(s)	496
SmlI	CTYRAG	2 cut(s)	1186, 1380
SmoI	CTYRAG	2 cut(s)	1186, 1380
SphI	GCATGC	2 cut(s)	89, 573
Sse9I	AATT	8 cut(s)	91, 288, 516, 726, 975, 1004, 1029, 1399
SsiI	CCGC	4 cut(s)	302, 440, 618, 1242
SspMI	CTAG	1 cut(s)	1419
StyD4I	CCNGG	2 cut(s)	162, 1445
StyI	CCWWGG	2 cut(s)	57, 248
TaaI	ACNGT	2 cut(s)	151, 1157
TaiI	ACGT	1 cut(s)	172
TaqI	TCGA	2 cut(s)	786, 1201
TasI	AATT	8 cut(s)	91, 288, 516, 726, 975, 1004, 1029, 1399
TatI	WGTACW	1 cut(s)	372
TauI	GCSGC	1 cut(s)	442
TfiI	GAWTC	4 cut(s)	180, 560, 593, 811
Tru1I	TTAA	7 cut(s)	243, 326, 651, 758, 846, 926, 1187
Tru9I	TTAA	7 cut(s)	243, 326, 651, 758, 846, 926, 1187
TscAI	CASTG	3 cut(s)	154, 1092, 1332
TseFI	GTSAC	2 cut(s)	803, 1151
TseI	GCWGC	4 cut(s)	502, 686, 1091, 1274
Tsp45I	GTSAC	2 cut(s)	803, 1151
TspDTI	ATGAA	6 cut(s)	332, 674, 977, 1035, 1107, 1392
TspRI	CASTG	3 cut(s)	154, 1092, 1332
Van91I	CCANNNNNTGG	1 cut(s)	962
Vha464I	CTTAAG	1 cut(s)	1186
VpaK11BI	GGWCC	1 cut(s)	1391
XapI	RAATTY	2 cut(s)	288, 516
XceI	RCATGY	3 cut(s)	89, 573, 1334
XcmI	CCANNNNNNNNNTGG	3 cut(s)	385, 1084, 1304
XspI	CTAG	1 cut(s)	1419
Zsp2I	ATGCAT	2 cut(s)	87, 575

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.