Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0002550

Physical Location & Seq

Genomic Coordinates Reverse (-)

596845 .. 605619

Gene Structure 8775 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00277160.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3204 bp

ATGTCTTCCTTGAGTAGGGAACTGGTGTTCTTGATTTTACAGTTCTTGGACGAGGAGAAGTTCAAGGAAACAGTTCATAAGCTAGAGCAAGAGTCTGGCTTTTTCTTCAATATGAAGCATTTTGAGGATCAAGTCCAGGCTGGAGAGTGGGACGAAGTCGAGCGATATTTGGGTGGCTTCACTAAAGTTGAAGACAATCGGTATTCAATGAAGATCTTCTTTGAAATCAGGAAGCAGAAGTATTTGGAAGCTCTCGATAGGCAGGATAGGGCGAAGGCTGTTGAGATTCTTGTGAAGGACCTTAAGGTTTTTGCATCTTTTAATGAGGAACTTTTCAAGGAGATCACTCAGTTGCTCACACTTGACAACTTCAGGCAAAATGAGCAGCTTTCCAAGTACGGGGACACAAAATCTGCTCGGAATATCATGCTTATAGAACTTAAAAAGCTCATTGAAGCGAACCCATTATTTCGTGATAAACTTGCATTTCCGTCCTTCAAAAGTTCACGGTTAAGGACTTTGATAAACCAGAGTCTCAACTGGCAGCACCAACTTTGCAAGAATCCCCGTCCAAATCCTGATATTAAAACACTATTTATGGATCATTCTTGCACTCCAAATGCTAATGGATCTCGTCCTCCTCCCACAAACAATCCCCTTGTTGGACCAATTCCAAAGGCTGGTGCATTTCCTCCTATTGGTGCCCATGGTCCATTTCAGCCTGTTGTCTCCCCTTCTCCTGGTGCTATTGCTGGGTGGATGTCAAACGCTAATCCTTCTATGCCTCACCCTGCTGTTGCAGCAGCTCCTCCTGGTCTTGTGCAGCCTTCTAGCGCAGCTGCTTTCTTAAAGCACCCAAGGACGCCGACAGGTGTTACAGGGATGGATTATCAATCAGCTGATTCAGAGCACTTGATTAAGCGTATTCGTACTGGCCCAGCTGATGAGGTGTCCTTTTCTGGGGTGATGCTTAGTTCCAATGCGTATTCACAAGATGACCTTCCGAAGGCTGTTGTTCGGACCCTTAGTCAAGGATCTAATGTAATGAGCATGGACTTTCATCCACAGCAACAAAATATTCTTCTAGTTGGGACAAATGTTGGCGACATCAGTCTTTGGGAATTAGGATCCCGGGAAAGGTTGGTCCATAAACCTTTCAAGGTCTGGGATATACAAGCTGCGTCAATGCCACTGCAGTCCAGTTTATGGAAGGATATAGAGTCGAGTCTTATGGGATTTTCTTCCATAACCAGGACTGCTTTGTTGAATGATGCTGCAATATCAGTGAATCGTTGTGTTTGGAGCCCAGATGGACTTATGCTTGGTGTTGCATTTTCTAAGCACATTGTCCAGATATATACTAACAATCCAACTGGAGAATTGAGACAACATTTGGAGATTGATGCTCACGTTGGCGGTGTTAATGACATTGCATTTGCTCATCCCAACAAGCAATTGTGTATTGTTACTTGCGGAGATGATAAAGTGATTAAGGTATGGGATGCTGCAGCCGGACGCAGACAATATACATTTGAAGGCCATGAAGCTCCTGTATATTCAGTGTGCCCTCATTACAAAGAAAATATACAATTCATTTTTTCAACAGCGATTGATGGGAAGATTAAAGCTTGGCTATACGATTGTTTGGGGTCAAGGGTGGACTATGATGCTCCTGGACTTTGGTGCACCATGATGGCTTATAGTGCCGATGGAACTAGACTCTTCTCATGTGGAACGAGTAAAGAAGGTGAATCTCATCTGGTGGAGTGGAATGAGAGCGAAGGGGCCATCAAACGGACATATTCTGGGTTTAGAAAACGCTCTTTGGATGTTGTCCAGTTTGACACAACAAGGAACCGGTTCCTAGCCGCTGGTGAGGAATTTCAAATAAAGTTCTGGGATATGGACAATACCAATGTGCTGACAGCTGTTGATGCAGATGGCGGGTTGCCTGCTAGTCCTAGACTCAGATTCAACAAAGAAGGGTCCTTGTTAGCTGTAACAACGATTGACAGCGGTATAAAGATTTTAGCAAATAATGATGGTTTGCGCTTGATAAGGATGTTGGAGAGCAAAGCTATGGAGAGAAACCGAGGCACTTCTGAGCCCATCAACACTAAGCCTTTGATTGTCAATGCACTAGGCCCCATTGGGAATGTTTCTAACGCTGTTGCTCCAACCCTCGAACGTGCTGATAGAATCCAACCTGCTGTTTCTATCAGTAGTCTGGGTAATATGGAGAACAGCAGGCTTGTTGATGTTAAACCACGGATCCCTGATGATATAGACAAGATTAAGAGCTGGAAAATTTCTGATATTGCGGATCCTTCTCAAATGAAAGCTTTAAGATTGCCCGACTCTACAACAGCTGGAAAGGTTGTGAGACTAATGTACACGAATAATGGTTTGGCTCTGTTAGCCCTCGCTTCCAATGCTGTTCACAAGCTTTGGAAGTGGCCGCGTAATGACAGGAATAATCCGTCAGGGAAGGCTAGTGCATATGTTGTTCCACAGTTGTGGCAGCCTCCGAATGGAATTCTAATGGCTAATGATGTGAATGACAATAAACCAGCTGAGGAATCTACTGCATGCATTGCTTTATCTAAAAATGATTCTTACGTAATGTCTGCATCTGGCGGGAAGGTCTCTTTGTTCAACATGATGACATTTAAGGTCATGACAACATTTGTATCTCCCCCTCCTGCTGCCACTTTTCTGGCATTCCATCCTCAAGATAATAATATAATTGCTATTGGCATGGAGGACTCTACTATTTTAATATATAATGTCAGGGTTGACGAGGTCAAAACCAAACTTAAGGGTCACCAGAATCGGATAACGGGCCTTGCATTTTCCCAGACTCTTAACATACTGGTCTCTTCAGGAGCTGATGCCCAGTTATGTGTGTGGAGCATTGATGGGTGGGAGAAAAAGAAAACAAGGTTCATACAAGCACCAACTGGTCGGCAATCCCCATTGGTTGGAGAAACAAAAGTTCAGTTTCATAACGATCACACACATCTATTGGTTGCCCATGAAAGCCAAATTGCTGTGTATGACAGCAAGCTTGATTGTTTACGCTTTTGGTCTCCAAAAGATGCACTTGCTGCTCCCATCTCATGTGCAATATATTCATGCGATGGTTTGTTGGTATATGCTACTTTTTGTGACGGTGCTGTGGGAGTTTTGATGCTGATAGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003002 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009753 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009867 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009933 GO:0009987 GO:0010014 GO:0010016 GO:0010033 GO:0010051 GO:0010072 GO:0010154 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032870 GO:0042221 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045892 GO:0045934 GO:0046983 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048519 GO:0048523 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0080090 GO:0090421 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

1067

Amino Acids

118.25

Weight (kDa)

7.32

Isoelectric Point (pI)

41.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LisH_TPL	PF17814	5 - 34	6.3e-13	LisH-like dimerisation domain
TPR1-like_2nd	PF21889	37 - 161	1.8e-61	TPR1-like, CTLH-containing domain
zf_topless	PF21359	164 - 204	1.4e-25	TOPLESS, zinc finger domain
WD40_CDC20-Fz	PF24807	332 - 578	1.9e-09	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd	PF25172	410 - 581	6.7e-08	WDR3 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	420 - 579	1.2e-11	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	428 - 641	5.1e-20	WDR5 beta-propeller domain
WD40_Gbeta	PF25391	429 - 546	1e-05	G protein beta WD-40 repeat protein
Beta-prop_THOC3	PF25174	429 - 688	1.3e-20	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	443 - 545	4.1e-09	WDHD1 first WD40 domain
WD40_Prp19	PF24814	471 - 545	3.2e-07	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	854 - 977	1.9e-13	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	857 - 976	9.9e-17	WDR3 first beta-propeller domain
WD40_WDHD1_1st	PF24817	857 - 1060	6.4e-18	WDHD1 first WD40 domain
WDR55	PF24796	857 - 973	1.3e-06	WDR55
Beta-prop_EML_2	PF23414	858 - 978	7.8e-11	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5	PF25175	859 - 1060	3.9e-15	WDR5 beta-propeller domain
WD40_Prp19	PF24814	862 - 1022	2e-13	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	863 - 973	4.4e-10	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	864 - 980	2.1e-12	WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	875 - 974	5.9e-11	WDR36/Utp21 first beta-propeller
Beta-prop_IFT140_1st	PF23383	875 - 974	2.3e-11	IFT140 first beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	880 - 984	7.5e-09	WDR90/POC16, second beta-propeller
Beta-prop_RIG_1st	PF23770	889 - 955	2.3e-07	RIG first beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002550)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770
malus_domestica	MD01G1234100.v1.1 MD07G1306300.v1.1
prunus_persica	Prupe.2G324300_v2.0.a1
pyrus_communis	pycom01g24260 pycom07g27930
rosa_chinensis	RchiOBHm_Chr1g0382471 RchiOBHm_Chr1g0382481 RchiOBHm_Chr1g0383911
rosa_laevigata	RLG00000026087 RLG00000026174 RLG00000026177
rosa_multiflora	Rmu_sc0000110.1_g000051 Rmu_sc0003885.1_g000009 Rmu_sc0005139.1_g000028
rosa_roxburghii	Rroxscaffold_4G00277160 Rroxscaffold_4G00278020 Rroxscaffold_4G00278080
rosa_rugosa	Rorug01G0434600 Rorug01G0434900 Rorug01G0443000
rosa_samantha	Rh1AG459200 Rh1AG459300 Rh1AG459600 Rh1AG468900 Rh1BG415700 Rh1BG426600 Rh1CG429700 Rh1CG429800 Rh1CG439400 Rh1DG447800 Rh1DG447900 Rh1DG459400
rosa_wichuraiana	Rw1G040350 Rw1G041180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	2215
AccB1I	GGYRCC	1 cut(s)	701
AccB7I	CCANNNNNTGG	3 cut(s)	680, 1206, 2981
AccII	CGCG	1 cut(s)	2461
AciI	CCGC	8 cut(s)	1416, 1473, 1869, 1944, 2016, 2321, 2459, 2637
AcoI	YGGCCR	1 cut(s)	2456
AcsI	RAATTY	3 cut(s)	1880, 2307, 2535
AcuI	CTGAAG	2 cut(s)	355, 2865
AcyI	GRCGYC	1 cut(s)	863
AfaI	GTAC	3 cut(s)	398, 931, 2393
AflII	CTTAAG	2 cut(s)	302, 2816
AgeI	ACCGGT	1 cut(s)	1857
AhdI	GACNNNNNGTC	1 cut(s)	1026
AjnI	CCWGG	5 cut(s)	135, 739, 811, 1250, 1672
AjuI	GAANNNNNNNTTGG	2 cut(s)	2390, 2422
AleI	CACNNNNGTG	1 cut(s)	2515
AloI	GAACNNNNNNTCC	2 cut(s)	11, 43
Alw21I	GWGCWC	2 cut(s)	912, 1688
Alw26I	GTCTC	7 cut(s)	539, 733, 1378, 2377, 2650, 2881, 3093
Alw44I	GTGCAC	1 cut(s)	1684
AlwNI	CAGNNNCTG	1 cut(s)	2888
Ama87I	CYCGRG	1 cut(s)	1131
AoxI	GGCC	6 cut(s)	934, 1537, 1785, 2143, 2456, 2842
ApaLI	GTGCAC	1 cut(s)	1684
ApoI	RAATTY	3 cut(s)	1880, 2307, 2535
ArsI	GACNNNNNNTTYG	2 cut(s)	1418, 1450
AsiGI	ACCGGT	1 cut(s)	1857
Asp700I	GAANNNNTTC	3 cut(s)	72, 215, 1859
AspLEI	GCGC	2 cut(s)	836, 2052
AsuC2I	CCSGG	2 cut(s)	1132, 1133
AsuHPI	GGTGA	5 cut(s)	779, 976, 1760, 1886, 2816
AvaI	CYCGRG	1 cut(s)	1131
AvaII	GGWCC	6 cut(s)	298, 665, 710, 1020, 1144, 1986
BaeGI	GKGCMC	3 cut(s)	706, 1568, 1688
BaeI	ACNNNNGTAYC	2 cut(s)	2673, 2706
BamHI	GGATCC	3 cut(s)	1127, 2271, 2323
BanI	GGYRCC	1 cut(s)	701
BanII	GRGCYC	2 cut(s)	1307, 2109
BbsI	GAAGAC	1 cut(s)	198
Bbv12I	GWGCWC	2 cut(s)	912, 1688
BbvCI	CCTCAGC	1 cut(s)	2574
BciT130I	CCWGG	5 cut(s)	137, 741, 813, 1252, 1674
BcnI	CCSGG	2 cut(s)	1132, 1133
BcoDI	GTCTC	7 cut(s)	539, 733, 1378, 2377, 2650, 2881, 3093
BfaI	CTAG	9 cut(s)	83, 831, 1085, 1716, 1865, 1956, 1962, 2141, 2493
BfmI	CTRYAG	2 cut(s)	1193, 1506
BfrI	CTTAAG	2 cut(s)	302, 2816
BfuAI	ACCTGC	1 cut(s)	2215
BglII	AGATCT	1 cut(s)	213
Bme1390I	CCNGG	7 cut(s)	137, 741, 813, 1132, 1133, 1252, 1674
Bme18I	GGWCC	6 cut(s)	298, 665, 710, 1020, 1144, 1986
BmeRI	GACNNNNNGTC	1 cut(s)	1026
BmeT110I	CYCGRG	1 cut(s)	1131
BmrFI	CCNGG	7 cut(s)	137, 741, 813, 1132, 1133, 1252, 1674
BmrI	ACTGGG	1 cut(s)	2890
BmuI	ACTGGG	1 cut(s)	2890
BoxI	GACNNNNGTC	1 cut(s)	1110
BpiI	GAAGAC	1 cut(s)	198
BpmI	CTGGAG	2 cut(s)	162, 1395
Bpu10I	CCTNAGC	1 cut(s)	2574
BpuEI	CTTGAG	2 cut(s)	31, 2715
BpuMI	CCSGG	2 cut(s)	1132, 1133
BsaAI	YACGTR	1 cut(s)	2620
BsaHI	GRCGYC	1 cut(s)	863
BsaI	GGTCTC	3 cut(s)	2650, 2881, 3093
BsaJI	CCNNGG	5 cut(s)	706, 857, 1131, 2092, 2267
BsaWI	WCCGGW	1 cut(s)	1857
Bse118I	RCCGGY	1 cut(s)	1857
Bse1I	ACTGG	9 cut(s)	27, 545, 937, 1200, 1378, 1837, 2877, 2896, 2965
Bse3DI	GCAATG	2 cut(s)	1428, 2592
BseBI	CCWGG	5 cut(s)	137, 741, 813, 1252, 1674
BseDI	CCNNGG	5 cut(s)	706, 857, 1131, 2092, 2267
BseGI	GGATG	8 cut(s)	765, 888, 1060, 1441, 1507, 1834, 2067, 2725
BseMI	GCAATG	2 cut(s)	1428, 2592
BseMII	CTCAG	4 cut(s)	362, 1981, 2094, 2565
BseNI	ACTGG	9 cut(s)	27, 545, 937, 1200, 1378, 1837, 2877, 2896, 2965
BseRI	GAGGAG	3 cut(s)	68, 630, 798
BseSI	GKGCMC	3 cut(s)	706, 1568, 1688
BseYI	CCCAGC	2 cut(s)	752, 937
BsgI	GTGCAG	1 cut(s)	842
Bsh1236I	CGCG	1 cut(s)	2461
BshFI	GGCC	6 cut(s)	936, 1539, 1787, 2145, 2458, 2844
BshNI	GGYRCC	1 cut(s)	701
BshTI	ACCGGT	1 cut(s)	1857
BsiHKAI	GWGCWC	2 cut(s)	912, 1688
BsiHKCI	CYCGRG	1 cut(s)	1131
BsiSI	CCGG	3 cut(s)	1132, 1512, 1858
BslFI	GGGAC	3 cut(s)	164, 416, 1105
BsmAI	GTCTC	7 cut(s)	539, 733, 1378, 2377, 2650, 2881, 3093
BsmFI	GGGAC	3 cut(s)	164, 416, 1105
BsmI	GAATGC	1 cut(s)	2720
BsnI	GGCC	6 cut(s)	936, 1539, 1787, 2145, 2458, 2844
Bso31I	GGTCTC	3 cut(s)	2650, 2881, 3093
BsoBI	CYCGRG	1 cut(s)	1131
Bsp1286I	GDGCHC	6 cut(s)	706, 912, 1307, 1568, 1688, 2109
Bsp1407I	TGTACA	1 cut(s)	2391
Bsp19I	CCATGG	1 cut(s)	706
BspACI	CCGC	8 cut(s)	1416, 1473, 1869, 1944, 2016, 2321, 2459, 2637
BspANI	GGCC	6 cut(s)	936, 1539, 1787, 2145, 2458, 2844
BspCNI	CTCAG	4 cut(s)	361, 1980, 2095, 2566
BspFNI	CGCG	1 cut(s)	2461
BspHI	TCATGA	1 cut(s)	2676
BspMAI	CTGCAG	2 cut(s)	1197, 1510
BspMI	ACCTGC	1 cut(s)	2215
BspT107I	GGYRCC	1 cut(s)	701
BspTI	CTTAAG	2 cut(s)	302, 2816
BspTNI	GGTCTC	3 cut(s)	2650, 2881, 3093
BsrDI	GCAATG	2 cut(s)	1428, 2592
BsrFI	RCCGGY	1 cut(s)	1857
BsrGI	TGTACA	1 cut(s)	2391
BsrI	ACTGG	9 cut(s)	27, 545, 937, 1200, 1378, 1837, 2877, 2896, 2965
BssAI	RCCGGY	1 cut(s)	1857
BssECI	CCNNGG	5 cut(s)	706, 857, 1131, 2092, 2267
BssNI	GRCGYC	1 cut(s)	863
BssT1I	CCWWGG	2 cut(s)	706, 857
Bst2UI	CCWGG	5 cut(s)	137, 741, 813, 1252, 1674
Bst4CI	ACNGT	5 cut(s)	42, 73, 510, 2514, 3173
Bst6I	CTCTTC	2 cut(s)	1727, 2884
BstACI	GRCGYC	1 cut(s)	863
BstAFI	CTTAAG	2 cut(s)	302, 2816
BstAPI	GCANNNNNTGC	2 cut(s)	2594, 3107
BstAUI	TGTACA	1 cut(s)	2391
BstBAI	YACGTR	1 cut(s)	2620
BstC8I	GCNNGC	4 cut(s)	1953, 2249, 2590, 3065
BstDEI	CTNAG	8 cut(s)	348, 971, 1025, 1338, 1967, 2103, 2118, 2574
BstDSI	CCRYGG	2 cut(s)	706, 2267
BstEII	GGTNACC	1 cut(s)	2822
BstENI	CCTNNNNNAGG	2 cut(s)	13, 1004
BstF5I	GGATG	8 cut(s)	765, 888, 1060, 1441, 1507, 1834, 2067, 2725
BstFNI	CGCG	1 cut(s)	2461
BstHHI	GCGC	2 cut(s)	836, 2052
BstMAI	GTCTC	7 cut(s)	539, 733, 1378, 2377, 2650, 2881, 3093
BstMWI	GCNNNNNNNGC	8 cut(s)	382, 800, 1703, 1934, 2417, 2432, 2594, 3107
BstNI	CCWGG	5 cut(s)	137, 741, 813, 1252, 1674
BstNSI	RCATGY	1 cut(s)	2592
BstPAI	GACNNNNGTC	1 cut(s)	1110
BstPI	GGTNACC	1 cut(s)	2822
BstSCI	CCNGG	7 cut(s)	135, 739, 811, 1130, 1131, 1250, 1672
BstSFI	CTRYAG	2 cut(s)	1193, 1506
BstSLI	GKGCMC	3 cut(s)	706, 1568, 1688
BstSNI	TACGTA	1 cut(s)	2620
BstUI	CGCG	1 cut(s)	2461
BstV2I	GAAGAC	1 cut(s)	198
BstX2I	RGATCY	6 cut(s)	213, 629, 1034, 1127, 2271, 2323
BstXI	CCANNNNNNTGG	2 cut(s)	2517, 2716
BstYI	RGATCY	6 cut(s)	213, 629, 1034, 1127, 2271, 2323
BsuRI	GGCC	6 cut(s)	936, 1539, 1787, 2145, 2458, 2844
BtgI	CCRYGG	2 cut(s)	706, 2267
BtgZI	GCGATG	1 cut(s)	3153
BtsCI	GGATG	8 cut(s)	765, 888, 1060, 1441, 1507, 1834, 2067, 2725
BtsI	GCAGTG	1 cut(s)	1190
BtsIMutI	CAGTG	3 cut(s)	1190, 1290, 1566
BveI	ACCTGC	1 cut(s)	2215
Cac8I	GCNNGC	4 cut(s)	1953, 2249, 2590, 3065
CaiI	CAGNNNCTG	1 cut(s)	2888
CciI	TCATGA	1 cut(s)	2676
CfoI	GCGC	2 cut(s)	836, 2052
Cfr10I	RCCGGY	1 cut(s)	1857
Cfr9I	CCCGGG	1 cut(s)	1131
CseI	GACGC	3 cut(s)	871, 1170, 1524
Csp6I	GTAC	3 cut(s)	397, 930, 2392
CspAI	ACCGGT	1 cut(s)	1857
CviQI	GTAC	3 cut(s)	397, 930, 2392
DdeI	CTNAG	8 cut(s)	348, 971, 1025, 1338, 1967, 2103, 2118, 2574
DriI	GACNNNNNGTC	1 cut(s)	1026
EaeI	YGGCCR	1 cut(s)	2456
Eam1104I	CTCTTC	2 cut(s)	1727, 2884
Eam1105I	GACNNNNNGTC	1 cut(s)	1026
EarI	CTCTTC	2 cut(s)	1727, 2884
Eco105I	TACGTA	1 cut(s)	2620
Eco130I	CCWWGG	2 cut(s)	706, 857
Eco24I	GRGCYC	2 cut(s)	1307, 2109
Eco31I	GGTCTC	3 cut(s)	2650, 2881, 3093
Eco47I	GGWCC	6 cut(s)	298, 665, 710, 1020, 1144, 1986
Eco57I	CTGAAG	2 cut(s)	355, 2865
Eco88I	CYCGRG	1 cut(s)	1131
Eco91I	GGTNACC	1 cut(s)	2822
EcoNI	CCTNNNNNAGG	2 cut(s)	13, 1004
EcoO109I	RGGNCCY	3 cut(s)	298, 1986, 2144
EcoO65I	GGTNACC	1 cut(s)	2822
EcoRI	GAATTC	1 cut(s)	2535
EcoRII	CCWGG	5 cut(s)	135, 739, 811, 1250, 1672
EcoT14I	CCWWGG	2 cut(s)	706, 857
EcoT22I	ATGCAT	1 cut(s)	2594
EcoT38I	GRGCYC	2 cut(s)	1307, 2109
ErhI	CCWWGG	2 cut(s)	706, 857
FalI	AAGNNNNNCTT	6 cut(s)	203, 235, 984, 1016, 3087, 3119
FaqI	GGGAC	3 cut(s)	164, 416, 1105
FauI	CCCGC	2 cut(s)	1937, 2630
FauNDI	CATATG	1 cut(s)	2500
FokI	GGATG	8 cut(s)	772, 895, 1047, 1428, 1514, 1841, 2074, 2712
FriOI	GRGCYC	2 cut(s)	1307, 2109
FspBI	CTAG	9 cut(s)	83, 831, 1085, 1716, 1865, 1956, 1962, 2141, 2493
GlaI	GCGC	2 cut(s)	835, 2051
GsaI	CCCAGC	2 cut(s)	756, 941
GsuI	CTGGAG	2 cut(s)	162, 1395
HaeIII	GGCC	6 cut(s)	936, 1539, 1787, 2145, 2458, 2844
HapII	CCGG	3 cut(s)	1132, 1512, 1858
HgaI	GACGC	3 cut(s)	871, 1170, 1524
HhaI	GCGC	2 cut(s)	836, 2052
Hin1I	GRCGYC	1 cut(s)	863
Hin6I	GCGC	2 cut(s)	834, 2050
HinP1I	GCGC	2 cut(s)	834, 2050
HincII	GTYRAC	1 cut(s)	2797
HindII	GTYRAC	1 cut(s)	2797
HindIII	AAGCTT	4 cut(s)	1626, 2340, 2444, 3065
HpaII	CCGG	3 cut(s)	1132, 1512, 1858
HphI	GGTGA	5 cut(s)	779, 976, 1760, 1886, 2816
Hpy166II	GTNNAC	7 cut(s)	506, 1660, 1686, 2394, 2440, 2797, 3077
Hpy188I	TCNGA	9 cut(s)	420, 907, 1005, 1020, 1970, 2104, 2314, 2529, 2835
Hpy188III	TCNNGA	9 cut(s)	31, 229, 254, 473, 578, 1351, 2677, 2732, 2883
Hpy8I	GTNNAC	7 cut(s)	506, 1660, 1686, 2394, 2440, 2797, 3077
HpyCH4III	ACNGT	5 cut(s)	42, 73, 510, 2514, 3173
HpyCH4IV	ACGT	3 cut(s)	1410, 2188, 2619
HpyF10VI	GCNNNNNNNGC	8 cut(s)	382, 800, 1703, 1934, 2417, 2432, 2594, 3107
HpyF3I	CTNAG	8 cut(s)	348, 971, 1025, 1338, 1967, 2103, 2118, 2574
HpySE526I	ACGT	3 cut(s)	1410, 2188, 2619
Hsp92I	GRCGYC	1 cut(s)	863
HspAI	GCGC	2 cut(s)	834, 2050
LmnI	GCTCC	8 cut(s)	811, 1302, 1552, 1675, 2179, 2885, 2910, 3115
MaeI	CTAG	9 cut(s)	83, 831, 1085, 1716, 1865, 1956, 1962, 2141, 2493
MaeII	ACGT	3 cut(s)	1410, 2188, 2619
MaeIII	GTNAC	5 cut(s)	874, 1465, 1999, 2822, 3167
MboII	GAAGA	9 cut(s)	97, 203, 208, 223, 1073, 1233, 1630, 1714, 2871
MfeI	CAATTG	1 cut(s)	1454
MflI	RGATCY	6 cut(s)	213, 629, 1034, 1127, 2271, 2323
MhlI	GDGCHC	6 cut(s)	706, 912, 1307, 1568, 1688, 2109
MlyI	GAGTC	9 cut(s)	101, 541, 1229, 1234, 1713, 1959, 2351, 2759, 2854
MmeI	TCCRAC	6 cut(s)	643, 1394, 2046, 2201, 2227, 2962
Mph1103I	ATGCAT	1 cut(s)	2594
MroXI	GAANNNNTTC	3 cut(s)	72, 215, 1859
MslI	CAYNNNNRTG	2 cut(s)	1691, 2515
MspA1I	CMGCKG	8 cut(s)	839, 899, 941, 1871, 1928, 2016, 2369, 2573
MspCI	CTTAAG	2 cut(s)	302, 2816
MspI	CCGG	3 cut(s)	1132, 1512, 1858
MspR9I	CCNGG	7 cut(s)	137, 741, 813, 1132, 1133, 1252, 1674
MunI	CAATTG	1 cut(s)	1454
Mva1269I	GAATGC	1 cut(s)	2720
MvaI	CCWGG	5 cut(s)	137, 741, 813, 1252, 1674
MvnI	CGCG	1 cut(s)	2461
MwoI	GCNNNNNNNGC	8 cut(s)	382, 800, 1703, 1934, 2417, 2432, 2594, 3107
NciI	CCSGG	2 cut(s)	1132, 1133
NcoI	CCATGG	1 cut(s)	706
NdeI	CATATG	1 cut(s)	2500
NmuCI	GTSAC	2 cut(s)	2822, 3167
NsiI	ATGCAT	1 cut(s)	2594
NspI	RCATGY	1 cut(s)	2592
OliI	CACNNNNGTG	1 cut(s)	2515
PaeI	GCATGC	1 cut(s)	2592
PagI	TCATGA	1 cut(s)	2676
PctI	GAATGC	1 cut(s)	2720
PdmI	GAANNNNTTC	3 cut(s)	72, 215, 1859
PflFI	GACNNNGTC	2 cut(s)	155, 2801
PflMI	CCANNNNNTGG	3 cut(s)	680, 1206, 2981
PfoI	TCCNGGA	1 cut(s)	1672
PinAI	ACCGGT	1 cut(s)	1857
PleI	GAGTC	9 cut(s)	100, 540, 1228, 1233, 1713, 1959, 2351, 2759, 2854
PpsI	GAGTC	9 cut(s)	100, 540, 1228, 1233, 1713, 1959, 2351, 2759, 2854
Ppu21I	YACGTR	1 cut(s)	2620
PpuMI	RGGWCCY	2 cut(s)	298, 1986
PshAI	GACNNNNGTC	1 cut(s)	1110
Psp5II	RGGWCCY	2 cut(s)	298, 1986
Psp6I	CCWGG	5 cut(s)	135, 739, 811, 1250, 1672
PspEI	GGTNACC	1 cut(s)	2822
PspFI	CCCAGC	2 cut(s)	752, 937
PspGI	CCWGG	5 cut(s)	135, 739, 811, 1250, 1672
PspPPI	RGGWCCY	2 cut(s)	298, 1986
PstI	CTGCAG	2 cut(s)	1197, 1510
PstNI	CAGNNNCTG	1 cut(s)	2888
PsuI	RGATCY	6 cut(s)	213, 629, 1034, 1127, 2271, 2323
PsyI	GACNNNGTC	2 cut(s)	155, 2801
PvuII	CAGCTG	6 cut(s)	839, 899, 941, 1928, 2369, 2573
RsaI	GTAC	3 cut(s)	398, 931, 2393
RsaNI	GTAC	3 cut(s)	397, 930, 2392
RseI	CAYNNNNRTG	2 cut(s)	1691, 2515
SchI	GAGTC	9 cut(s)	101, 541, 1229, 1234, 1713, 1959, 2351, 2759, 2854
ScrFI	CCNGG	7 cut(s)	137, 741, 813, 1132, 1133, 1252, 1674
SduI	GDGCHC	6 cut(s)	706, 912, 1307, 1568, 1688, 2109
SfcI	CTRYAG	2 cut(s)	1193, 1506
SinI	GGWCC	6 cut(s)	298, 665, 710, 1020, 1144, 1986
SmaI	CCCGGG	1 cut(s)	1133
SmiMI	CAYNNNNRTG	2 cut(s)	1691, 2515
SmlI	CTYRAG	4 cut(s)	10, 302, 2730, 2816
SmoI	CTYRAG	4 cut(s)	10, 302, 2730, 2816
SnaBI	TACGTA	1 cut(s)	2620
SphI	GCATGC	1 cut(s)	2592
SsiI	CCGC	8 cut(s)	1416, 1473, 1869, 1944, 2016, 2321, 2459, 2637
SspI	AATATT	1 cut(s)	1078
SspMI	CTAG	9 cut(s)	83, 831, 1085, 1716, 1865, 1956, 1962, 2141, 2493
StyD4I	CCNGG	7 cut(s)	135, 739, 811, 1130, 1131, 1250, 1672
StyI	CCWWGG	2 cut(s)	706, 857
TaaI	ACNGT	5 cut(s)	42, 73, 510, 2514, 3173
TaiI	ACGT	3 cut(s)	1413, 2191, 2622
TaqI	TCGA	4 cut(s)	159, 255, 1223, 2184
TatI	WGTACW	1 cut(s)	2391
TauI	GCSGC	2 cut(s)	1871, 2461
TscAI	CASTG	3 cut(s)	1197, 1290, 1566
TseFI	GTSAC	2 cut(s)	2822, 3167
Tsp45I	GTSAC	2 cut(s)	2822, 3167
TspGWI	ACGGA	4 cut(s)	480, 1810, 2284, 2469
TspMI	CCCGGG	1 cut(s)	1131
TspRI	CASTG	3 cut(s)	1197, 1290, 1566
Tth111I	GACNNNGTC	2 cut(s)	155, 2801
Van91I	CCANNNNNTGG	3 cut(s)	680, 1206, 2981
Vha464I	CTTAAG	2 cut(s)	302, 2816
VneI	GTGCAC	1 cut(s)	1684
VpaK11BI	GGWCC	6 cut(s)	298, 665, 710, 1020, 1144, 1986
XagI	CCTNNNNNAGG	2 cut(s)	13, 1004
XapI	RAATTY	3 cut(s)	1880, 2307, 2535
XceI	RCATGY	1 cut(s)	2592
XmaI	CCCGGG	1 cut(s)	1131
XmnI	GAANNNNTTC	3 cut(s)	72, 215, 1859
XspI	CTAG	9 cut(s)	83, 831, 1085, 1716, 1865, 1956, 1962, 2141, 2493
Zsp2I	ATGCAT	1 cut(s)	2594

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.