MD02G1072800.v1.1

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001660
Physical Location & Seq
Reverse (-)
5894302 .. 5896388
2087 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1072800.v1.1.491

Sequence Viewer

Length: 468 bp
ATGAATCCAGGCGCGATGGCTACCGTTCCGGGACAACTGATCTGGGAGATCGTGAAGAAGAACAACTCCTTCTTGGTGAAGGAGTTCGGCCGCGGCAACGCCGGCGTCCGCTTCAGCAAGGAGCCCAACAACCTCTACAACCTCCACTCCTACAAGCATTCCGGTTTGGCGAACAAGAAGACGGTGACTATTCAGGCTGGAGGGAAAGACCAGTCTGTGGTGCTCGCCACCACCAAGACCAAGAAGCAGAACAAGCCCGCCAGTCTGCTTCACAAGTCTGTCATCAGGAAGGAGTTTCCCCGCGTCGCCAAGGCTGTTACCAATCAGGTTGCGGATAACTTCTACAGACCGGATCTGAAGAAAGCTGCCCTTGCCAGGCTCAGCGCCGTTCACAGGAGCCTCAGGGTTGCCAAGTCTGGTGCGAAGAAGAGGAACAGGCAGGCTGTCAAGACCCCTGGTAGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L28e

Protein Analysis

156

Amino Acids

17.11

Weight (kDa)

11.2

Isoelectric Point (pI)

26.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L28e PF01778 13 - 133 8.3e-38 Ribosomal L28e protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 217
AccII CGCG 3 cut(s) 14, 93, 303
AciI CCGC 6 cut(s) 91, 93, 109, 258, 301, 332
AclWI GGATC 1 cut(s) 360
AcoI YGGCCR 1 cut(s) 88
AcuI CTGAAG 2 cut(s) 97, 377
AcyI GRCGYC 1 cut(s) 105
AfiI CCNNNNNNNGG 4 cut(s) 217, 375, 393, 459
AjnI CCWGG 3 cut(s) 7, 374, 454
AluBI AGCT 1 cut(s) 365
AluI AGCT 1 cut(s) 365
Alw21I GWGCWC 1 cut(s) 225
AlwI GGATC 1 cut(s) 360
AoxI GGCC 1 cut(s) 88
ApeKI GCWGC 1 cut(s) 365
Asp700I GAANNNNTTC 1 cut(s) 83
AspLEI GCGC 2 cut(s) 14, 386
AsuC2I CCSGG 1 cut(s) 30
AsuHPI GGTGA 2 cut(s) 88, 196
AxyI CCTNAGG 1 cut(s) 401
BanII GRGCYC 1 cut(s) 126
BbsI GAAGAC 1 cut(s) 185
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 1 cut(s) 352
BccI CCATC 1 cut(s) 10
BceAI ACGGC 1 cut(s) 371
BciT130I CCWGG 3 cut(s) 9, 376, 456
BcnI CCSGG 1 cut(s) 30
BfmI CTRYAG 1 cut(s) 343
BfoI RGCGCY 1 cut(s) 387
BisI GCNGC 3 cut(s) 91, 94, 366
BlpI GCTNAGC 1 cut(s) 380
BlsI GCNGC 3 cut(s) 92, 95, 367
Bme1390I CCNGG 4 cut(s) 9, 30, 376, 456
BmiI GGNNCC 2 cut(s) 123, 398
BmrFI CCNGG 4 cut(s) 9, 30, 376, 456
BpiI GAAGAC 1 cut(s) 185
BpmI CTGGAG 1 cut(s) 219
Bpu1102I GCTNAGC 1 cut(s) 380
BpuMI CCSGG 1 cut(s) 30
BsaHI GRCGYC 1 cut(s) 105
BsaJI CCNNGG 3 cut(s) 91, 309, 454
BsaWI WCCGGW 2 cut(s) 161, 349
BsaXI ACNNNNNCTCC 2 cut(s) 131, 161
Bsc4I CCNNNNNNNGG 4 cut(s) 217, 375, 393, 459
Bse118I RCCGGY 1 cut(s) 101
Bse1I ACTGG 2 cut(s) 211, 261
Bse21I CCTNAGG 1 cut(s) 401
BseBI CCWGG 3 cut(s) 9, 376, 456
BseDI CCNNGG 3 cut(s) 91, 309, 454
BseLI CCNNNNNNNGG 4 cut(s) 217, 375, 393, 459
BseMII CTCAG 2 cut(s) 394, 415
BseNI ACTGG 2 cut(s) 211, 261
BseX3I CGGCCG 1 cut(s) 88
BseXI GCAGC 1 cut(s) 352
Bsh1236I CGCG 3 cut(s) 14, 93, 303
Bsh1285I CGRYCG 1 cut(s) 91
BshFI GGCC 1 cut(s) 90
BsiEI CGRYCG 1 cut(s) 91
BsiHKAI GWGCWC 1 cut(s) 225
BsiSI CCGG 4 cut(s) 29, 102, 162, 350
BslFI GGGAC 1 cut(s) 45
BslI CCNNNNNNNGG 4 cut(s) 217, 375, 393, 459
BsmFI GGGAC 1 cut(s) 45
BsmI GAATGC 1 cut(s) 157
BsnI GGCC 1 cut(s) 90
Bsp1286I GDGCHC 2 cut(s) 126, 225
Bsp143I GATC 3 cut(s) 39, 48, 352
Bsp1720I GCTNAGC 1 cut(s) 380
BspACI CCGC 6 cut(s) 91, 93, 109, 258, 301, 332
BspANI GGCC 1 cut(s) 90
BspCNI CTCAG 2 cut(s) 393, 414
BspFNI CGCG 3 cut(s) 14, 93, 303
BspLI GGNNCC 2 cut(s) 123, 398
BspPI GGATC 1 cut(s) 360
BsrFI RCCGGY 1 cut(s) 101
BsrI ACTGG 2 cut(s) 211, 261
BssAI RCCGGY 1 cut(s) 101
BssECI CCNNGG 3 cut(s) 91, 309, 454
BssMI GATC 3 cut(s) 39, 48, 352
BssNI GRCGYC 1 cut(s) 105
BssT1I CCWWGG 1 cut(s) 309
Bst2UI CCWGG 3 cut(s) 9, 376, 456
Bst4CI ACNGT 2 cut(s) 25, 184
Bst6I CTCTTC 1 cut(s) 422
BstACI GRCGYC 1 cut(s) 105
BstC8I GCNNGC 4 cut(s) 103, 225, 258, 441
BstDEI CTNAG 2 cut(s) 380, 401
BstDSI CCRYGG 1 cut(s) 91
BstFNI CGCG 3 cut(s) 14, 93, 303
BstH2I RGCGCY 1 cut(s) 387
BstHHI GCGC 2 cut(s) 14, 386
BstKTI GATC 3 cut(s) 42, 51, 355
BstMBI GATC 3 cut(s) 39, 48, 352
BstMCI CGRYCG 1 cut(s) 91
BstMWI GCNNNNNNNGC 3 cut(s) 102, 253, 371
BstNI CCWGG 3 cut(s) 9, 376, 456
BstSCI CCNGG 4 cut(s) 7, 28, 374, 454
BstSFI CTRYAG 1 cut(s) 343
BstUI CGCG 3 cut(s) 14, 93, 303
BstV1I GCAGC 1 cut(s) 352
BstV2I GAAGAC 1 cut(s) 185
BstX2I RGATCY 1 cut(s) 352
BstYI RGATCY 1 cut(s) 352
BstZI CGGCCG 1 cut(s) 88
Bsu36I CCTNAGG 1 cut(s) 401
BsuRI GGCC 1 cut(s) 90
BtgI CCRYGG 1 cut(s) 91
BtgZI GCGATG 1 cut(s) 29
Cac8I GCNNGC 4 cut(s) 103, 225, 258, 441
CfoI GCGC 2 cut(s) 14, 386
Cfr10I RCCGGY 1 cut(s) 101
Cfr42I CCGCGG 1 cut(s) 94
CseI GACGC 2 cut(s) 94, 292
DdeI CTNAG 2 cut(s) 380, 401
DpnI GATC 3 cut(s) 41, 50, 354
DpnII GATC 3 cut(s) 39, 48, 352
EaeI YGGCCR 1 cut(s) 88
EagI CGGCCG 1 cut(s) 88
Eam1104I CTCTTC 1 cut(s) 422
EarI CTCTTC 1 cut(s) 422
EclXI CGGCCG 1 cut(s) 88
Eco130I CCWWGG 1 cut(s) 309
Eco24I GRGCYC 1 cut(s) 126
Eco52I CGGCCG 1 cut(s) 88
Eco57I CTGAAG 2 cut(s) 97, 377
Eco81I CCTNAGG 1 cut(s) 401
EcoRII CCWGG 3 cut(s) 7, 374, 454
EcoT14I CCWWGG 1 cut(s) 309
EcoT38I GRGCYC 1 cut(s) 126
ErhI CCWWGG 1 cut(s) 309
FalI AAGNNNNNCTT 2 cut(s) 354, 386
FaqI GGGAC 1 cut(s) 45
FauI CCCGC 2 cut(s) 265, 308
Fnu4HI GCNGC 3 cut(s) 91, 94, 366
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 3 cut(s) 91, 94, 366
GlaI GCGC 2 cut(s) 13, 385
GluI GCNGC 3 cut(s) 91, 94, 366
GsuI CTGGAG 1 cut(s) 219
HaeII RGCGCY 1 cut(s) 387
HaeIII GGCC 1 cut(s) 90
HapII CCGG 4 cut(s) 29, 102, 162, 350
HgaI GACGC 2 cut(s) 94, 292
HhaI GCGC 2 cut(s) 14, 386
Hin1I GRCGYC 1 cut(s) 105
Hin6I GCGC 2 cut(s) 12, 384
HinP1I GCGC 2 cut(s) 12, 384
HinfI GANTC 1 cut(s) 4
HpaII CCGG 4 cut(s) 29, 102, 162, 350
HphI GGTGA 2 cut(s) 88, 196
Hpy166II GTNNAC 1 cut(s) 391
Hpy188I TCNGA 1 cut(s) 357
Hpy188III TCNNGA 3 cut(s) 52, 286, 448
Hpy8I GTNNAC 1 cut(s) 391
Hpy99I CGWCG 1 cut(s) 308
HpyAV CCTTC 3 cut(s) 73, 79, 283
HpyCH4III ACNGT 2 cut(s) 25, 184
HpyF10VI GCNNNNNNNGC 3 cut(s) 102, 253, 371
HpyF3I CTNAG 2 cut(s) 380, 401
Hsp92I GRCGYC 1 cut(s) 105
HspAI GCGC 2 cut(s) 12, 384
KroI GCCGGC 1 cut(s) 101
KroNI GCCGGC 1 cut(s) 103
KspI CCGCGG 1 cut(s) 94
Kzo9I GATC 3 cut(s) 39, 48, 352
LmnI GCTCC 2 cut(s) 121, 396
Lsp1109I GCAGC 1 cut(s) 352
MaeIII GTNAC 2 cut(s) 184, 316
MalI GATC 3 cut(s) 41, 50, 354
MboI GATC 3 cut(s) 39, 48, 352
MboII GAAGA 6 cut(s) 67, 70, 190, 370, 436, 439
MflI RGATCY 1 cut(s) 352
MhlI GDGCHC 2 cut(s) 126, 225
MnlI CCTC 5 cut(s) 143, 152, 194, 410, 423
MreI CGCCGGCG 1 cut(s) 101
MroNI GCCGGC 1 cut(s) 101
MroXI GAANNNNTTC 1 cut(s) 83
MspA1I CMGCKG 1 cut(s) 93
MspI CCGG 4 cut(s) 29, 102, 162, 350
MspR9I CCNGG 4 cut(s) 9, 30, 376, 456
Mva1269I GAATGC 1 cut(s) 157
MvaI CCWGG 3 cut(s) 9, 376, 456
MvnI CGCG 3 cut(s) 14, 93, 303
MwoI GCNNNNNNNGC 3 cut(s) 102, 253, 371
NaeI GCCGGC 1 cut(s) 103
NciI CCSGG 1 cut(s) 30
NdeII GATC 3 cut(s) 39, 48, 352
NgoMIV GCCGGC 1 cut(s) 101
NlaIV GGNNCC 2 cut(s) 123, 398
NmuCI GTSAC 1 cut(s) 184
PctI GAATGC 1 cut(s) 157
PdiI GCCGGC 1 cut(s) 103
PdmI GAANNNNTTC 1 cut(s) 83
PfeI GAWTC 1 cut(s) 4
PflMI CCANNNNNTGG 1 cut(s) 217
PfoI TCCNGGA 1 cut(s) 28
PkrI GCNGC 3 cut(s) 92, 95, 367
Psp6I CCWGG 3 cut(s) 7, 374, 454
PspGI CCWGG 3 cut(s) 7, 374, 454
PspN4I GGNNCC 2 cut(s) 123, 398
PsuI RGATCY 1 cut(s) 352
SacII CCGCGG 1 cut(s) 94
SatI GCNGC 3 cut(s) 91, 94, 366
Sau3AI GATC 3 cut(s) 39, 48, 352
ScrFI CCNGG 4 cut(s) 9, 30, 376, 456
SduI GDGCHC 2 cut(s) 126, 225
SetI ASST 4 cut(s) 135, 144, 330, 367
SfcI CTRYAG 1 cut(s) 343
Sfr303I CCGCGG 1 cut(s) 94
SgrAI CRCCGGYG 1 cut(s) 101
SgrBI CCGCGG 1 cut(s) 94
SsiI CCGC 6 cut(s) 91, 93, 109, 258, 301, 332
StyD4I CCNGG 4 cut(s) 7, 28, 374, 454
StyI CCWWGG 1 cut(s) 309
TaaI ACNGT 2 cut(s) 25, 184
TauI GCSGC 2 cut(s) 93, 96
TfiI GAWTC 1 cut(s) 4
TseFI GTSAC 1 cut(s) 184
TseI GCWGC 1 cut(s) 365
Tsp45I GTSAC 1 cut(s) 184
TspDTI ATGAA 1 cut(s) 17
Van91I CCANNNNNTGG 1 cut(s) 217
XmnI GAANNNNTTC 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.