MD15G1202600.v1.1

60s ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001660
Physical Location & Seq
Reverse (-)
16126506 .. 16128552
2047 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1202600.v1.1.491

Sequence Viewer

Length: 480 bp
ATGATACAGCAGCGGAATCCAGGCGCCATGACAACCGTTCCGGGACAACTGATCTGGGAGATCGTGAAGAAGAACAACTCCTTCTTGGTGAAGGAGTTTGGCCGCGGCAACGCCGGTGTCCGCTTCAGCAAGGAGCCCAACAACCTCTACAACCTCCATTCCTACAAGCATTCCGGTTTGGCGAACAAGAAGACGGTTACTATTCAGGCAGGAGGCAAAGACCAGTCTGTGGTGCTCGCCACCACCAAGACCAAGAAGCAGAACAAGCCCGCCAGTCTGCTCCACAAGTCTGTGATCCGAAAGGAGTTTCCCCGCGCCGCCAAGGCTGTTACCAATCAGGTTGCGGATAACTTCTACAGACCTGATTTGAAGAGAGCTGCCCTTGCCAGGCTCAGTGCCGTTCACAAGAGTCTCAGGGTTGCCAAGTCCGGTGTGAAGAAGAGGAACAGGCAGGCTGTCAAGACCCCCGGTAGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L28e

Protein Analysis

160

Amino Acids

17.67

Weight (kDa)

11.33

Isoelectric Point (pI)

28.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L28e PF01778 17 - 137 1.1e-37 Ribosomal L28e protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 23
AccB7I CCANNNNNTGG 1 cut(s) 229
AccII CGCG 2 cut(s) 105, 315
AciI CCGC 8 cut(s) 13, 103, 105, 121, 270, 313, 318, 344
AclWI GGATC 1 cut(s) 289
AcoI YGGCCR 1 cut(s) 100
AcuI CTGAAG 1 cut(s) 109
AcyI GRCGYC 1 cut(s) 24
AfiI CCNNNNNNNGG 3 cut(s) 229, 387, 471
AgsI TTSAA 1 cut(s) 370
AjnI CCWGG 2 cut(s) 19, 386
AluBI AGCT 1 cut(s) 377
AluI AGCT 1 cut(s) 377
Alw21I GWGCWC 1 cut(s) 237
Alw26I GTCTC 1 cut(s) 416
AlwI GGATC 1 cut(s) 289
AoxI GGCC 1 cut(s) 100
ApeKI GCWGC 2 cut(s) 10, 377
AspLEI GCGC 2 cut(s) 26, 317
AsuC2I CCSGG 2 cut(s) 42, 468
AsuHPI GGTGA 1 cut(s) 100
BanI GGYRCC 1 cut(s) 23
BanII GRGCYC 1 cut(s) 138
BbsI GAAGAC 1 cut(s) 197
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 2 cut(s) 22, 364
BceAI ACGGC 1 cut(s) 383
BciT130I CCWGG 2 cut(s) 21, 388
BcnI CCSGG 2 cut(s) 42, 468
BcoDI GTCTC 1 cut(s) 416
BfmI CTRYAG 1 cut(s) 355
BfoI RGCGCY 1 cut(s) 27
BglI GCCNNNNNGGC 1 cut(s) 323
BisI GCNGC 5 cut(s) 11, 103, 106, 318, 378
BlsI GCNGC 5 cut(s) 12, 104, 107, 319, 379
Bme1390I CCNGG 4 cut(s) 21, 42, 388, 468
BmiI GGNNCC 2 cut(s) 25, 135
BmrFI CCNGG 4 cut(s) 21, 42, 388, 468
BpiI GAAGAC 1 cut(s) 197
BpuMI CCSGG 2 cut(s) 42, 468
BsaHI GRCGYC 1 cut(s) 24
BsaJI CCNNGG 3 cut(s) 103, 321, 466
BsaWI WCCGGW 2 cut(s) 173, 428
Bsc4I CCNNNNNNNGG 3 cut(s) 229, 387, 471
Bse118I RCCGGY 1 cut(s) 113
Bse1I ACTGG 2 cut(s) 223, 273
BseBI CCWGG 2 cut(s) 21, 388
BseDI CCNNGG 3 cut(s) 103, 321, 466
BseLI CCNNNNNNNGG 3 cut(s) 229, 387, 471
BseMII CTCAG 2 cut(s) 406, 427
BseNI ACTGG 2 cut(s) 223, 273
BseXI GCAGC 2 cut(s) 22, 364
Bsh1236I CGCG 2 cut(s) 105, 315
BshFI GGCC 1 cut(s) 102
BshNI GGYRCC 1 cut(s) 23
BsiHKAI GWGCWC 1 cut(s) 237
BsiSI CCGG 5 cut(s) 41, 114, 174, 429, 468
BslFI GGGAC 1 cut(s) 57
BslI CCNNNNNNNGG 3 cut(s) 229, 387, 471
BsmAI GTCTC 1 cut(s) 416
BsmFI GGGAC 1 cut(s) 57
BsmI GAATGC 1 cut(s) 169
BsnI GGCC 1 cut(s) 102
Bsp1286I GDGCHC 2 cut(s) 138, 237
Bsp143I GATC 3 cut(s) 51, 60, 294
BspACI CCGC 8 cut(s) 13, 103, 105, 121, 270, 313, 318, 344
BspANI GGCC 1 cut(s) 102
BspCNI CTCAG 2 cut(s) 405, 426
BspFNI CGCG 2 cut(s) 105, 315
BspLI GGNNCC 2 cut(s) 25, 135
BspPI GGATC 1 cut(s) 289
BspT107I GGYRCC 1 cut(s) 23
BsrFI RCCGGY 1 cut(s) 113
BsrI ACTGG 2 cut(s) 223, 273
BssAI RCCGGY 1 cut(s) 113
BssECI CCNNGG 3 cut(s) 103, 321, 466
BssMI GATC 3 cut(s) 51, 60, 294
BssNI GRCGYC 1 cut(s) 24
BssT1I CCWWGG 1 cut(s) 321
Bst2UI CCWGG 2 cut(s) 21, 388
Bst4CI ACNGT 2 cut(s) 37, 196
Bst6I CTCTTC 2 cut(s) 365, 434
BstACI GRCGYC 1 cut(s) 24
BstC8I GCNNGC 3 cut(s) 237, 270, 453
BstDEI CTNAG 2 cut(s) 392, 413
BstDSI CCRYGG 1 cut(s) 103
BstFNI CGCG 2 cut(s) 105, 315
BstH2I RGCGCY 1 cut(s) 27
BstHHI GCGC 2 cut(s) 26, 317
BstKTI GATC 3 cut(s) 54, 63, 297
BstMAI GTCTC 1 cut(s) 416
BstMBI GATC 3 cut(s) 51, 60, 294
BstMWI GCNNNNNNNGC 3 cut(s) 265, 323, 383
BstNI CCWGG 2 cut(s) 21, 388
BstSCI CCNGG 4 cut(s) 19, 40, 386, 466
BstSFI CTRYAG 1 cut(s) 355
BstUI CGCG 2 cut(s) 105, 315
BstV1I GCAGC 2 cut(s) 22, 364
BstV2I GAAGAC 1 cut(s) 197
BsuRI GGCC 1 cut(s) 102
BtgI CCRYGG 1 cut(s) 103
BtsIMutI CAGTG 1 cut(s) 400
Cac8I GCNNGC 3 cut(s) 237, 270, 453
CfoI GCGC 2 cut(s) 26, 317
Cfr10I RCCGGY 1 cut(s) 113
Cfr42I CCGCGG 1 cut(s) 106
CviAII CATG 1 cut(s) 28
CviJI RGCY 7 cut(s) 102, 136, 268, 326, 377, 391, 455
CviKI_1 RGCY 7 cut(s) 102, 136, 268, 326, 377, 391, 455
DdeI CTNAG 2 cut(s) 392, 413
DinI GGCGCC 1 cut(s) 25
DpnI GATC 3 cut(s) 53, 62, 296
DpnII GATC 3 cut(s) 51, 60, 294
EaeI YGGCCR 1 cut(s) 100
Eam1104I CTCTTC 2 cut(s) 365, 434
EarI CTCTTC 2 cut(s) 365, 434
Eco130I CCWWGG 1 cut(s) 321
Eco24I GRGCYC 1 cut(s) 138
Eco57I CTGAAG 1 cut(s) 109
EcoRII CCWGG 2 cut(s) 19, 386
EcoT14I CCWWGG 1 cut(s) 321
EcoT38I GRGCYC 1 cut(s) 138
EgeI GGCGCC 1 cut(s) 25
EheI GGCGCC 1 cut(s) 25
ErhI CCWWGG 1 cut(s) 321
FaeI CATG 1 cut(s) 31
FaiI YATR 1 cut(s) 29
FaqI GGGAC 1 cut(s) 57
FatI CATG 1 cut(s) 27
FauI CCCGC 2 cut(s) 277, 320
Fnu4HI GCNGC 5 cut(s) 11, 103, 106, 318, 378
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 5 cut(s) 11, 103, 106, 318, 378
GlaI GCGC 2 cut(s) 25, 316
GluI GCNGC 5 cut(s) 11, 103, 106, 318, 378
HaeII RGCGCY 1 cut(s) 27
HaeIII GGCC 1 cut(s) 102
HapII CCGG 5 cut(s) 41, 114, 174, 429, 468
HhaI GCGC 2 cut(s) 26, 317
Hin1I GRCGYC 1 cut(s) 24
Hin1II CATG 1 cut(s) 31
Hin6I GCGC 2 cut(s) 24, 315
HinP1I GCGC 2 cut(s) 24, 315
HinfI GANTC 2 cut(s) 16, 409
HpaII CCGG 5 cut(s) 41, 114, 174, 429, 468
HphI GGTGA 1 cut(s) 100
Hpy166II GTNNAC 1 cut(s) 403
Hpy188I TCNGA 1 cut(s) 299
Hpy188III TCNNGA 2 cut(s) 64, 460
Hpy8I GTNNAC 1 cut(s) 403
HpyAV CCTTC 2 cut(s) 85, 91
HpyCH4III ACNGT 2 cut(s) 37, 196
HpyF10VI GCNNNNNNNGC 3 cut(s) 265, 323, 383
HpyF3I CTNAG 2 cut(s) 392, 413
Hsp92I GRCGYC 1 cut(s) 24
Hsp92II CATG 1 cut(s) 31
HspAI GCGC 2 cut(s) 24, 315
KasI GGCGCC 1 cut(s) 23
KspI CCGCGG 1 cut(s) 106
Kzo9I GATC 3 cut(s) 51, 60, 294
LmnI GCTCC 2 cut(s) 133, 285
Lsp1109I GCAGC 2 cut(s) 22, 364
MaeIII GTNAC 2 cut(s) 196, 328
MalI GATC 3 cut(s) 53, 62, 296
MboI GATC 3 cut(s) 51, 60, 294
MboII GAAGA 6 cut(s) 79, 82, 202, 382, 448, 451
MhlI GDGCHC 2 cut(s) 138, 237
Mly113I GGCGCC 1 cut(s) 24
MlyI GAGTC 1 cut(s) 418
MnlI CCTC 4 cut(s) 155, 164, 206, 435
MspA1I CMGCKG 2 cut(s) 13, 105
MspI CCGG 5 cut(s) 41, 114, 174, 429, 468
MspR9I CCNGG 4 cut(s) 21, 42, 388, 468
Mva1269I GAATGC 1 cut(s) 169
MvaI CCWGG 2 cut(s) 21, 388
MvnI CGCG 2 cut(s) 105, 315
MwoI GCNNNNNNNGC 3 cut(s) 265, 323, 383
NarI GGCGCC 1 cut(s) 24
NciI CCSGG 2 cut(s) 42, 468
NdeII GATC 3 cut(s) 51, 60, 294
NlaIII CATG 1 cut(s) 31
NlaIV GGNNCC 2 cut(s) 25, 135
PctI GAATGC 1 cut(s) 169
PfeI GAWTC 1 cut(s) 16
PflMI CCANNNNNTGG 1 cut(s) 229
PfoI TCCNGGA 1 cut(s) 40
PkrI GCNGC 5 cut(s) 12, 104, 107, 319, 379
PleI GAGTC 1 cut(s) 417
PluTI GGCGCC 1 cut(s) 27
PpsI GAGTC 1 cut(s) 417
Psp6I CCWGG 2 cut(s) 19, 386
PspGI CCWGG 2 cut(s) 19, 386
PspN4I GGNNCC 2 cut(s) 25, 135
SacII CCGCGG 1 cut(s) 106
SatI GCNGC 5 cut(s) 11, 103, 106, 318, 378
Sau3AI GATC 3 cut(s) 51, 60, 294
SchI GAGTC 1 cut(s) 418
ScrFI CCNGG 4 cut(s) 21, 42, 388, 468
SduI GDGCHC 2 cut(s) 138, 237
SetI ASST 5 cut(s) 147, 156, 342, 364, 379
SfcI CTRYAG 1 cut(s) 355
SfoI GGCGCC 1 cut(s) 25
Sfr303I CCGCGG 1 cut(s) 106
SgrAI CRCCGGYG 1 cut(s) 113
SgrBI CCGCGG 1 cut(s) 106
SsiI CCGC 8 cut(s) 13, 103, 105, 121, 270, 313, 318, 344
SspDI GGCGCC 1 cut(s) 23
StyD4I CCNGG 4 cut(s) 19, 40, 386, 466
StyI CCWWGG 1 cut(s) 321
TaaI ACNGT 2 cut(s) 37, 196
TauI GCSGC 3 cut(s) 105, 108, 320
TfiI GAWTC 1 cut(s) 16
TscAI CASTG 1 cut(s) 400
TseI GCWGC 2 cut(s) 10, 377
TspRI CASTG 1 cut(s) 400
Van91I CCANNNNNTGG 1 cut(s) 229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.