MD02G1150200.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003684
Physical Location & Seq
Reverse (-)
12347422 .. 12350590
3169 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1150200.v1.1.491

Sequence Viewer

Length: 1527 bp
ATGGATAACGATAAAGATGTTGGCAGCGACTCTGACTCTTATGTCATTGTGAGCATTGCCAATTCTGTGTGCAGTGATTCTGAAAGTGTCGCGGATAGTGATTTTGAAAATTATGAAATTGTGAGTCCTAAAAACAATGTGGCTACTGATTCTGGAGAAGATCTATTTGATGGAAGCACACAACAGTCGAAGAATGTGGAAAAGGACGGTTATGCGTGTTCTGACTTTGATTGTGGTGAGGGCACTGATTCTGAAGAAGTTCTATCTGATGAAAGCCAACAACATTTGAAGAAAGTGGATATTGATAGTGATCTGAGTACCGATTCTGAAAAAGAACTATCTGATGGATTCAGAAGAAAGTTGAAGATTATGGATGATCACCATTACATGGATACTGTTCTCACTGGGAAACAGCTGAAGATGAGACACTGTTCTTGTGAGTTTCACAAATATCCATGTAAAAGAATCGGTCCTAAACTTTCCCATGATGAAAGGCTTTATAAGAGAATGGACATAGGTGACGTTGCAATGGAGAATGTTCTTCCTTTCCTCCCTGCAAAAATGCTGTGCAGGTTGAGAACTGTTAGCAAACAGTGGGATAAGTGGATTTCTAATCCTTTCTTCATTCATAAGCAAACTTACTACTTCAGGAAAATCTCTGGTCTTTTTTGTCAAGCCCCTGGTGGCGACCCTTACTTCCTTCCACTTGATAATGATTCTTACGGCGTTCCTAGATCTTACCTCAGCTTCATGCCCGAACCAGTTATTGCAAAGACCACTTGCAATGGTCTGGTCTGCTGTCAGAGCAATGACGGAGAAGATACCTACTACATTTGCAACCCTGTGAACAGGCAATTTAGAAAGCTTCCCAAGCCTGATTTGTATCACGGATCTGAGTCTTATCCGGCACTAGCCTTTGAGCCTTCTGTGCTTGACTTTGAAGTGCATTTTCAACTTGTCTGTGCCTTCTCTGTACCCGGTCATTTGGGTGTCTATTTTGATATATACTCTTCAAGGACAAGCGGTTGGGAACTCTCCGAGACAGTATGCTATGAGCTGGAGGCTACAACGTTGGAAGGTAACACCCTCTTTCTAAAGGGTGTACTCTTTTGGGAAACTTTAACGGGTGTCATTCTGGCATTTGACTTGAAGACGATGCAATATAGTATTCTGTCACTCCCCTTCAGCACGGGACCACGTGGTGTTATTTCACAGAGGAATGGAGAGCTGTGTTACATTCTGCCTCGTGATGAATCCAGTATGTACTCCTTGGAGATCTACGGTAATATGGACATGAGCCTGAAGTGGAAGATTAATCTCTTTGACCAGAGTTTCTCCTCTTATATGGACACATTTCGGGTATTGGCTTGTGTGAATGATGATGTGATGATACTTCTTGTCGGACAGACGGTGATTGCATATCATGTGAAAGTCCCGGAGGTAAAAGTATTGAAAGGAAACGATTACGGGCTTGGTGCTGGAAATGAAAGATATCTTCCATATGTGAACAGCCTTGTGCAGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

509

Amino Acids

57.53

Weight (kDa)

4.81

Isoelectric Point (pI)

42.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
b-prop_At3g26010-like PF24750 243 - 431 3e-10 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 501
AasI GACNNNNNNGTC 1 cut(s) 41
Acc36I ACCTGC 1 cut(s) 561
AccB7I CCANNNNNTGG 1 cut(s) 388
AccII CGCG 1 cut(s) 92
AciI CCGC 2 cut(s) 92, 1023
AclI AACGTT 1 cut(s) 1070
AclWI GGATC 1 cut(s) 898
AcuI CTGAAG 5 cut(s) 273, 437, 631, 1168, 1322
AcvI CACGTG 1 cut(s) 1199
AdeI CACNNNGTG 1 cut(s) 1202
AfaI GTAC 4 cut(s) 319, 975, 1104, 1265
AfiI CCNNNNNNNGG 1 cut(s) 388
AgsI TTSAA 8 cut(s) 107, 289, 364, 941, 953, 1014, 1150, 1453
AjnI CCWGG 1 cut(s) 679
AluBI AGCT 5 cut(s) 415, 747, 865, 1057, 1228
AluI AGCT 5 cut(s) 415, 747, 865, 1057, 1228
Alw26I GTCTC 2 cut(s) 418, 1034
AlwI GGATC 1 cut(s) 898
ApeKI GCWGC 1 cut(s) 24
AseI ATTAAT 1 cut(s) 1314
Asp700I GAANNNNTTC 1 cut(s) 258
AspS9I GGNCC 2 cut(s) 470, 1193
AsuC2I CCSGG 2 cut(s) 978, 1436
AsuHPI GGTGA 4 cut(s) 248, 371, 530, 1423
AvaII GGWCC 2 cut(s) 470, 1193
BaeGI GKGCMC 1 cut(s) 245
BauI CACGAG 1 cut(s) 1245
BbrPI CACGTG 1 cut(s) 1199
BbsI GAAGAC 1 cut(s) 1157
BbvCI CCTCAGC 1 cut(s) 743
BbvI GCAGC 1 cut(s) 36
BccI CCATC 2 cut(s) 164, 338
BceAI ACGGC 1 cut(s) 739
BciT130I CCWGG 1 cut(s) 681
BciVI GTATCC 1 cut(s) 385
BclI TGATCA 1 cut(s) 376
BcnI CCSGG 2 cut(s) 978, 1436
BcoDI GTCTC 2 cut(s) 418, 1034
BfaI CTAG 3 cut(s) 732, 911, 1525
BfuAI ACCTGC 1 cut(s) 561
BfuI GTATCC 1 cut(s) 385
BglII AGATCT 4 cut(s) 160, 734, 1275, 1521
BisI GCNGC 1 cut(s) 25
BlsI GCNGC 1 cut(s) 26
Bme1390I CCNGG 3 cut(s) 681, 978, 1436
Bme18I GGWCC 2 cut(s) 470, 1193
BmgT120I GGNCC 2 cut(s) 470, 1193
BmiI GGNNCC 1 cut(s) 1194
BmrFI CCNGG 3 cut(s) 681, 978, 1436
BmrI ACTGGG 1 cut(s) 414
BmsI GCATC 1 cut(s) 1146
BmuI ACTGGG 1 cut(s) 414
BpiI GAAGAC 1 cut(s) 1157
BpmI CTGGAG 2 cut(s) 174, 1079
Bpu10I CCTNAGC 1 cut(s) 743
BpuMI CCSGG 2 cut(s) 978, 1436
BsaAI YACGTR 1 cut(s) 1199
BsaBI GATNNNNATC 2 cut(s) 309, 882
BsaJI CCNNGG 2 cut(s) 679, 1269
Bsc4I CCNNNNNNNGG 1 cut(s) 388
Bse1I ACTGG 3 cut(s) 409, 761, 1257
Bse3DI GCAATG 4 cut(s) 54, 534, 790, 814
Bse8I GATNNNNATC 2 cut(s) 309, 882
BseBI CCWGG 1 cut(s) 681
BseDI CCNNGG 2 cut(s) 679, 1269
BseGI GGATG 1 cut(s) 379
BseJI GATNNNNATC 2 cut(s) 309, 882
BseLI CCNNNNNNNGG 1 cut(s) 388
BseMI GCAATG 4 cut(s) 54, 534, 790, 814
BseMII CTCAG 3 cut(s) 305, 757, 885
BseNI ACTGG 3 cut(s) 409, 761, 1257
BseRI GAGGAG 1 cut(s) 1327
BseSI GKGCMC 1 cut(s) 245
BseXI GCAGC 1 cut(s) 36
BsgI GTGCAG 2 cut(s) 91, 589
Bsh1236I CGCG 1 cut(s) 92
BsiSI CCGG 3 cut(s) 905, 978, 1436
BslFI GGGAC 2 cut(s) 1206, 1418
BslI CCNNNNNNNGG 1 cut(s) 388
BsmAI GTCTC 2 cut(s) 418, 1034
BsmFI GGGAC 2 cut(s) 1206, 1418
Bsp1286I GDGCHC 1 cut(s) 245
Bsp143I GATC 7 cut(s) 160, 310, 376, 734, 890, 1275, 1521
BspACI CCGC 2 cut(s) 92, 1023
BspCNI CTCAG 3 cut(s) 306, 756, 886
BspFNI CGCG 1 cut(s) 92
BspLI GGNNCC 1 cut(s) 1194
BspMI ACCTGC 1 cut(s) 561
BspPI GGATC 1 cut(s) 898
BsrDI GCAATG 4 cut(s) 54, 534, 790, 814
BsrI ACTGG 3 cut(s) 409, 761, 1257
BssECI CCNNGG 2 cut(s) 679, 1269
BssMI GATC 7 cut(s) 160, 310, 376, 734, 890, 1275, 1521
BssSI CACGAG 1 cut(s) 1245
BssT1I CCWWGG 1 cut(s) 1269
Bst2BI CACGAG 1 cut(s) 1245
Bst2UI CCWGG 1 cut(s) 681
Bst4CI ACNGT 9 cut(s) 186, 209, 397, 431, 583, 594, 1045, 1283, 1411
Bst6I CTCTTC 1 cut(s) 1015
BstBAI YACGTR 1 cut(s) 1199
BstDEI CTNAG 3 cut(s) 314, 743, 894
BstF5I GGATG 1 cut(s) 379
BstFNI CGCG 1 cut(s) 92
BstKTI GATC 7 cut(s) 163, 313, 379, 737, 893, 1278, 1524
BstMAI GTCTC 2 cut(s) 418, 1034
BstMBI GATC 7 cut(s) 160, 310, 376, 734, 890, 1275, 1521
BstMWI GCNNNNNNNGC 3 cut(s) 804, 871, 928
BstNI CCWGG 1 cut(s) 681
BstSCI CCNGG 3 cut(s) 679, 976, 1434
BstSLI GKGCMC 1 cut(s) 245
BstUI CGCG 1 cut(s) 92
BstV1I GCAGC 1 cut(s) 36
BstV2I GAAGAC 1 cut(s) 1157
BstX2I RGATCY 5 cut(s) 160, 734, 890, 1275, 1521
BstYI RGATCY 5 cut(s) 160, 734, 890, 1275, 1521
BsuI GTATCC 1 cut(s) 385
BtsCI GGATG 1 cut(s) 379
BtsI GCAGTG 1 cut(s) 79
BtsIMutI CAGTG 5 cut(s) 79, 243, 402, 427, 599
BveI ACCTGC 1 cut(s) 561
Cfr13I GGNCC 2 cut(s) 470, 1193
Csp6I GTAC 4 cut(s) 318, 974, 1103, 1264
CviAII CATG 6 cut(s) 388, 456, 485, 751, 1294, 1424
CviQI GTAC 4 cut(s) 318, 974, 1103, 1264
DdeI CTNAG 3 cut(s) 314, 743, 894
DpnI GATC 7 cut(s) 162, 312, 378, 736, 892, 1277, 1523
DpnII GATC 7 cut(s) 160, 310, 376, 734, 890, 1275, 1521
DraIII CACNNNGTG 1 cut(s) 1202
DrdI GACNNNNNNGTC 1 cut(s) 41
DseDI GACNNNNNNGTC 1 cut(s) 41
Eam1104I CTCTTC 1 cut(s) 1015
EarI CTCTTC 1 cut(s) 1015
Eco130I CCWWGG 1 cut(s) 1269
Eco32I GATATC 1 cut(s) 1493
Eco47I GGWCC 2 cut(s) 470, 1193
Eco57I CTGAAG 5 cut(s) 273, 437, 631, 1168, 1322
Eco72I CACGTG 1 cut(s) 1199
EcoRII CCWGG 1 cut(s) 679
EcoRV GATATC 1 cut(s) 1493
EcoT14I CCWWGG 1 cut(s) 1269
ErhI CCWWGG 1 cut(s) 1269
FaeI CATG 6 cut(s) 391, 459, 488, 754, 1297, 1427
FaqI GGGAC 2 cut(s) 1206, 1418
FatI CATG 6 cut(s) 387, 455, 484, 750, 1293, 1423
FauNDI CATATG 1 cut(s) 1501
FbaI TGATCA 1 cut(s) 376
Fnu4HI GCNGC 1 cut(s) 25
FokI GGATG 1 cut(s) 386
Fsp4HI GCNGC 1 cut(s) 25
FspBI CTAG 3 cut(s) 732, 911, 1525
GluI GCNGC 1 cut(s) 25
GsuI CTGGAG 2 cut(s) 174, 1079
HapII CCGG 3 cut(s) 905, 978, 1436
Hin1II CATG 6 cut(s) 391, 459, 488, 754, 1297, 1427
HindIII AAGCTT 1 cut(s) 863
HpaII CCGG 3 cut(s) 905, 978, 1436
HphI GGTGA 4 cut(s) 248, 371, 530, 1423
Hpy166II GTNNAC 3 cut(s) 847, 1103, 1507
Hpy188III TCNNGA 3 cut(s) 153, 649, 1247
Hpy8I GTNNAC 3 cut(s) 847, 1103, 1507
HpyAV CCTTC 5 cut(s) 710, 933, 976, 1070, 1192
HpyCH4III ACNGT 9 cut(s) 186, 209, 397, 431, 583, 594, 1045, 1283, 1411
HpyCH4IV ACGT 3 cut(s) 522, 1070, 1198
HpyF10VI GCNNNNNNNGC 3 cut(s) 804, 871, 928
HpyF3I CTNAG 3 cut(s) 314, 743, 894
HpySE526I ACGT 3 cut(s) 522, 1070, 1198
Hsp92II CATG 6 cut(s) 391, 459, 488, 754, 1297, 1427
Ksp22I TGATCA 1 cut(s) 376
Kzo9I GATC 7 cut(s) 160, 310, 376, 734, 890, 1275, 1521
Lsp1109I GCAGC 1 cut(s) 36
LweI GCATC 1 cut(s) 1146
MaeI CTAG 3 cut(s) 732, 911, 1525
MaeII ACGT 3 cut(s) 522, 1070, 1198
MaeIII GTNAC 4 cut(s) 518, 1079, 1173, 1232
MalI GATC 7 cut(s) 162, 312, 378, 736, 892, 1277, 1523
MboI GATC 7 cut(s) 160, 310, 376, 734, 890, 1275, 1521
MflI RGATCY 5 cut(s) 160, 734, 890, 1275, 1521
MhlI GDGCHC 1 cut(s) 245
MluCI AATT 4 cut(s) 61, 109, 117, 854
MlyI GAGTC 4 cut(s) 23, 29, 133, 905
MmeI TCCRAC 2 cut(s) 1053, 1381
MnlI CCTC 9 cut(s) 232, 560, 752, 1054, 1097, 1209, 1254, 1348, 1432
MroXI GAANNNNTTC 1 cut(s) 258
MseI TTAA 2 cut(s) 1121, 1314
MslI CAYNNNNRTG 1 cut(s) 987
MspA1I CMGCKG 1 cut(s) 415
MspI CCGG 3 cut(s) 905, 978, 1436
MspR9I CCNGG 3 cut(s) 681, 978, 1436
MvaI CCWGG 1 cut(s) 681
MvnI CGCG 1 cut(s) 92
MwoI GCNNNNNNNGC 3 cut(s) 804, 871, 928
NciI CCSGG 2 cut(s) 978, 1436
NdeI CATATG 1 cut(s) 1501
NdeII GATC 7 cut(s) 160, 310, 376, 734, 890, 1275, 1521
NlaIII CATG 6 cut(s) 391, 459, 488, 754, 1297, 1427
NlaIV GGNNCC 1 cut(s) 1194
NmuCI GTSAC 2 cut(s) 518, 1173
PdmI GAANNNNTTC 1 cut(s) 258
PfeI GAWTC 8 cut(s) 77, 149, 248, 323, 348, 465, 716, 1253
PflMI CCANNNNNTGG 1 cut(s) 388
PfoI TCCNGGA 1 cut(s) 1434
PkrI GCNGC 1 cut(s) 26
PleI GAGTC 4 cut(s) 23, 29, 132, 904
PmaCI CACGTG 1 cut(s) 1199
PmlI CACGTG 1 cut(s) 1199
PpsI GAGTC 4 cut(s) 23, 29, 132, 904
Ppu21I YACGTR 1 cut(s) 1199
PshBI ATTAAT 1 cut(s) 1314
PsiI TTATAA 1 cut(s) 501
Psp1406I AACGTT 1 cut(s) 1070
Psp6I CCWGG 1 cut(s) 679
PspCI CACGTG 1 cut(s) 1199
PspGI CCWGG 1 cut(s) 679
PspN4I GGNNCC 1 cut(s) 1194
PspPI GGNCC 2 cut(s) 470, 1193
PsuI RGATCY 5 cut(s) 160, 734, 890, 1275, 1521
PvuII CAGCTG 1 cut(s) 415
RsaI GTAC 4 cut(s) 319, 975, 1104, 1265
RsaNI GTAC 4 cut(s) 318, 974, 1103, 1264
RseI CAYNNNNRTG 1 cut(s) 987
SaqAI TTAA 2 cut(s) 1121, 1314
SatI GCNGC 1 cut(s) 25
Sau3AI GATC 7 cut(s) 160, 310, 376, 734, 890, 1275, 1521
Sau96I GGNCC 2 cut(s) 470, 1193
SchI GAGTC 4 cut(s) 23, 29, 133, 905
ScrFI CCNGG 3 cut(s) 681, 978, 1436
SduI GDGCHC 1 cut(s) 245
SfaNI GCATC 1 cut(s) 1146
SinI GGWCC 2 cut(s) 470, 1193
SmiMI CAYNNNNRTG 1 cut(s) 987
Sse9I AATT 4 cut(s) 61, 109, 117, 854
SsiI CCGC 2 cut(s) 92, 1023
SspMI CTAG 3 cut(s) 732, 911, 1525
StyD4I CCNGG 3 cut(s) 679, 976, 1434
StyI CCWWGG 1 cut(s) 1269
TaaI ACNGT 9 cut(s) 186, 209, 397, 431, 583, 594, 1045, 1283, 1411
TaiI ACGT 3 cut(s) 525, 1073, 1201
TaqI TCGA 1 cut(s) 188
TaqII GACCGA 1 cut(s) 458
TasI AATT 4 cut(s) 61, 109, 117, 854
TatI WGTACW 2 cut(s) 1102, 1263
TfiI GAWTC 8 cut(s) 77, 149, 248, 323, 348, 465, 716, 1253
Tru1I TTAA 2 cut(s) 1121, 1314
Tru9I TTAA 2 cut(s) 1121, 1314
TscAI CASTG 5 cut(s) 79, 250, 409, 434, 599
TseFI GTSAC 2 cut(s) 518, 1173
TseI GCWGC 1 cut(s) 24
Tsp45I GTSAC 2 cut(s) 518, 1173
TspDTI ATGAA 8 cut(s) 129, 285, 504, 613, 617, 739, 1266, 1500
TspGWI ACGGA 2 cut(s) 828, 903
TspRI CASTG 5 cut(s) 79, 250, 409, 434, 599
Van91I CCANNNNNTGG 1 cut(s) 388
VpaK11BI GGWCC 2 cut(s) 470, 1193
VspI ATTAAT 1 cut(s) 1314
XmnI GAANNNNTTC 1 cut(s) 258
XspI CTAG 3 cut(s) 732, 911, 1525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.