RchiOBHm_Chr2g0101871

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003684
Physical Location & Seq
Reverse (-)
13321323 .. 13324219
2897 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47638

Sequence Viewer

Length: 1506 bp
ATGAACCCAAATCCTGTTAGTCTGATCCTCTGTTTAACTGCTCTCTCAACCGTGAATTCAGATTCTCTCGCTCTAAATTCACCACCGCATTTGATCTCCGGCCGCCTCTCTCAGCCCTACGACGTAATGGCGACTTCTGAGGAGGAGCATCCCGTTGATAATGACAATCTTGTGGACAATGATTCTGAAGAAGATCTGTTTGATGACATTGCAGAACAGCATATAGAGTCTTCAAATGAGCTCAAAGTGGTGGATCTCTCTGATGTGGTATGTTCAAATGAGCTCAAAGTGGTGGATCGCTCTGATGTGTTTGGCAGAGATCCTCGCAAAAGAACCGCTCCTGTAGATGTTCCTTATGATGAATTTCCACACAAAAGGTTTAAGCAAAACATGGAGATCCAAGATGTTGTAATGGAGCATGCTCTTTATTTTCTCCCTGCCAAGTCGCTTTGCAGATTCAAAACTGTTAACAAAGAGTGGGATCGGTACATTAACAGTCCCTTTTTCGCTCACCAGCAAACTCACTCCTTCCGAGACATCTCTGGTCTTTTTTGTCAACTCCCTGGTGATAAGCCTTCCTTCATATCACTCGATCAAGATGCTTATGGAATTCCTAGTCCATCCCTCAGTTTCTTGCCTCAGCCTGTTACTCTCAGAACCGCTTGCAATGGTCTGCTTTGCTGCCAAAACTGCTTTGAACAAAATAGCTACTACATTTGTAACCCTGTGAATGAGGAATGGAAAGTGCTTCCGGAGCCAAACTTTTATCATGGACCTGATCAATCAGCCTTGGCACTAGCCTTTGAGCCTTCGGCACTAAACTTTGCAGCACATTTTGAACTTGTGTGTGCATTTTCTCTATCTTTAACTGATCAACCAGTCATCTGCTTTGAGATATACTCTTCAAGGTCAAGGTCCTGGAGACTCGCTGAGACTGTATGTTCTGAGCTAGATTGCTTGAAGTTGAATGGGGATGGAATCTTCCTGAAGGGTGTAGTCTTTTGGGAAACTTTTGCTGGGGCCATTCTGGCTTTTGACTTGAAGGAGGAGCACTATGGCATTCTGTCACTCCCTCCCAACAGTGGACCGCAAGGTGTTCTAACAGAGATGCGGGGTGAGCTGTGCTATATTCTGCCTGTTAGAGAAGGTAATAGTTACACCTTGGAAATCTATGGTGATATGGACATGAATCTCAAGCATATAATTCCTCTCAATCTTGGTTTTCTTTCTCATACTCACCAAGAGTCGATAAGGGTTTTGGCTTGTGTGAATGATGATGCTGTGGTAATTCTTTTTGGAGACAGAGTGATTGCTTATAATATGAAAGCTAGGACGGTTGAAACATTAAGGGATATAGTGAATGGTAATTACAATGGTGAATACAATGCTGGATTTGCAAAGCATCTTCCTTATGTGAACAGCCTTGTTCATGTACACTCATCATACATAACGAGCCTCCTTTCTGAAGATCCTACTACCTCCTATTCCTATCTGAAGTCTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

501

Amino Acids

56.35

Weight (kDa)

4.78

Isoelectric Point (pI)

44.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_3 PF08268 219 - 388 1.4e-06 F-box associated beta propeller domain
FBA_1 PF07734 220 - 391 3.3e-12 F-box associated beta propeller domain
b-prop_At3g26010-like PF24750 220 - 391 7.6e-09 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1317
AccBSI CCGCTC 1 cut(s) 338
AccIII TCCGGA 1 cut(s) 751
AciI CCGC 6 cut(s) 86, 103, 336, 660, 1088, 1111
AclWI GGATC 7 cut(s) 19, 261, 303, 314, 391, 489, 1463
AcoI YGGCCR 1 cut(s) 100
AcsI RAATTY 4 cut(s) 55, 76, 362, 609
AcuI CTGAAG 3 cut(s) 207, 1007, 1485
AfaI GTAC 2 cut(s) 488, 1434
AfiI CCNNNNNNNGG 1 cut(s) 1082
AjnI CCWGG 2 cut(s) 562, 917
AluBI AGCT 6 cut(s) 241, 283, 708, 949, 1120, 1328
AluI AGCT 6 cut(s) 241, 283, 708, 949, 1120, 1328
Alw21I GWGCWC 3 cut(s) 243, 285, 1053
Alw26I GTCTC 4 cut(s) 528, 916, 926, 1293
AlwI GGATC 7 cut(s) 19, 261, 303, 314, 391, 489, 1463
Aor13HI TCCGGA 1 cut(s) 751
AoxI GGCC 2 cut(s) 100, 1020
ApeKI GCWGC 2 cut(s) 681, 827
ApoI RAATTY 4 cut(s) 55, 76, 362, 609
AspS9I GGNCC 4 cut(s) 773, 915, 1020, 1085
AsuHPI GGTGA 7 cut(s) 72, 503, 578, 1127, 1187, 1229, 1388
AvaII GGWCC 3 cut(s) 773, 915, 1085
BanII GRGCYC 2 cut(s) 243, 285
BbsI GAAGAC 1 cut(s) 222
Bbv12I GWGCWC 3 cut(s) 243, 285, 1053
BbvCI CCTCAGC 1 cut(s) 639
BbvI GCAGC 2 cut(s) 668, 839
BccI CCATC 2 cut(s) 628, 968
BcgI CGANNNNNNTGC 2 cut(s) 581, 615
BciT130I CCWGG 2 cut(s) 564, 919
BclI TGATCA 2 cut(s) 778, 871
BcoDI GTCTC 4 cut(s) 528, 916, 926, 1293
BfaI CTAG 4 cut(s) 615, 797, 950, 1329
BfmI CTRYAG 1 cut(s) 342
BglI GCCNNNNNGGC 1 cut(s) 1028
BglII AGATCT 1 cut(s) 193
BisI GCNGC 3 cut(s) 103, 682, 828
BlsI GCNGC 3 cut(s) 104, 683, 829
Bme1390I CCNGG 2 cut(s) 564, 919
Bme18I GGWCC 3 cut(s) 773, 915, 1085
BmgT120I GGNCC 4 cut(s) 773, 915, 1020, 1085
BmiI GGNNCC 2 cut(s) 756, 1021
BmrFI CCNGG 2 cut(s) 564, 919
BmsI GCATC 5 cut(s) 157, 589, 1098, 1267, 1411
BpiI GAAGAC 1 cut(s) 222
BplI GAGNNNNNCTC 2 cut(s) 884, 916
BpmI CTGGAG 1 cut(s) 940
Bpu10I CCTNAGC 1 cut(s) 639
BpuEI CTTGAG 1 cut(s) 1178
BsaJI CCNNGG 3 cut(s) 562, 789, 1161
BsaWI WCCGGW 1 cut(s) 751
BsaXI ACNNNNNCTCC 2 cut(s) 1290, 1320
Bsc4I CCNNNNNNNGG 1 cut(s) 1082
Bse1I ACTGG 1 cut(s) 878
Bse3DI GCAATG 2 cut(s) 207, 673
BseAI TCCGGA 1 cut(s) 751
BseBI CCWGG 2 cut(s) 564, 919
BseDI CCNNGG 3 cut(s) 562, 789, 1161
BseGI GGATG 3 cut(s) 148, 620, 979
BseLI CCNNNNNNNGG 1 cut(s) 1082
BseMI GCAATG 2 cut(s) 207, 673
BseMII CTCAG 7 cut(s) 125, 129, 640, 653, 667, 921, 936
BseNI ACTGG 1 cut(s) 878
BseRI GAGGAG 3 cut(s) 155, 158, 1061
BseX3I CGGCCG 1 cut(s) 100
BseXI GCAGC 2 cut(s) 668, 839
BseYI CCCAGC 1 cut(s) 1016
Bsh1285I CGRYCG 1 cut(s) 103
BshFI GGCC 2 cut(s) 102, 1022
BsiEI CGRYCG 1 cut(s) 103
BsiHKAI GWGCWC 3 cut(s) 243, 285, 1053
BsiSI CCGG 2 cut(s) 99, 752
BslFI GGGAC 1 cut(s) 483
BslI CCNNNNNNNGG 1 cut(s) 1082
BsmAI GTCTC 4 cut(s) 528, 916, 926, 1293
BsmFI GGGAC 1 cut(s) 483
BsmI GAATGC 1 cut(s) 1059
BsnI GGCC 2 cut(s) 102, 1022
Bsp1286I GDGCHC 3 cut(s) 243, 285, 1053
Bsp13I TCCGGA 1 cut(s) 751
Bsp1407I TGTACA 1 cut(s) 1432
BspACI CCGC 6 cut(s) 86, 103, 336, 660, 1088, 1111
BspANI GGCC 2 cut(s) 102, 1022
BspCNI CTCAG 7 cut(s) 124, 130, 639, 652, 666, 922, 937
BspEI TCCGGA 1 cut(s) 751
BspLI GGNNCC 2 cut(s) 756, 1021
BspPI GGATC 7 cut(s) 19, 261, 303, 314, 391, 489, 1463
BsrBI CCGCTC 1 cut(s) 338
BsrDI GCAATG 2 cut(s) 207, 673
BsrGI TGTACA 1 cut(s) 1432
BsrI ACTGG 1 cut(s) 878
BssECI CCNNGG 3 cut(s) 562, 789, 1161
BssT1I CCWWGG 2 cut(s) 789, 1161
Bst2UI CCWGG 2 cut(s) 564, 919
Bst4CI ACNGT 6 cut(s) 52, 466, 497, 937, 1082, 1336
Bst6I CTCTTC 1 cut(s) 907
BstAUI TGTACA 1 cut(s) 1432
BstC8I GCNNGC 2 cut(s) 420, 664
BstDEI CTNAG 7 cut(s) 111, 138, 626, 639, 653, 930, 945
BstF5I GGATG 3 cut(s) 148, 620, 979
BstMAI GTCTC 4 cut(s) 528, 916, 926, 1293
BstMCI CGRYCG 1 cut(s) 103
BstMWI GCNNNNNNNGC 5 cut(s) 690, 754, 1028, 1117, 1394
BstNI CCWGG 2 cut(s) 564, 919
BstNSI RCATGY 1 cut(s) 422
BstSCI CCNGG 2 cut(s) 562, 917
BstSFI CTRYAG 1 cut(s) 342
BstV1I GCAGC 2 cut(s) 668, 839
BstV2I GAAGAC 1 cut(s) 222
BstX2I RGATCY 5 cut(s) 193, 253, 319, 396, 1468
BstYI RGATCY 5 cut(s) 193, 253, 319, 396, 1468
BstZI CGGCCG 1 cut(s) 100
BsuRI GGCC 2 cut(s) 102, 1022
BtsCI GGATG 3 cut(s) 148, 620, 979
BtsIMutI CAGTG 1 cut(s) 1087
Cac8I GCNNGC 2 cut(s) 420, 664
Cfr13I GGNCC 4 cut(s) 773, 915, 1020, 1085
Csp6I GTAC 2 cut(s) 487, 1433
CspCI CAANNNNNGTGG 2 cut(s) 72, 107
CviAII CATG 5 cut(s) 391, 419, 770, 1186, 1430
CviQI GTAC 2 cut(s) 487, 1433
DdeI CTNAG 7 cut(s) 111, 138, 626, 639, 653, 930, 945
EaeI YGGCCR 1 cut(s) 100
EagI CGGCCG 1 cut(s) 100
Eam1104I CTCTTC 1 cut(s) 907
EarI CTCTTC 1 cut(s) 907
Ecl136II GAGCTC 2 cut(s) 241, 283
EclXI CGGCCG 1 cut(s) 100
Eco130I CCWWGG 2 cut(s) 789, 1161
Eco24I GRGCYC 2 cut(s) 243, 285
Eco47I GGWCC 3 cut(s) 773, 915, 1085
Eco52I CGGCCG 1 cut(s) 100
Eco53kI GAGCTC 2 cut(s) 241, 283
Eco57I CTGAAG 3 cut(s) 207, 1007, 1485
EcoICRI GAGCTC 2 cut(s) 241, 283
EcoO109I RGGNCCY 1 cut(s) 915
EcoRI GAATTC 2 cut(s) 55, 609
EcoRII CCWGG 2 cut(s) 562, 917
EcoT14I CCWWGG 2 cut(s) 789, 1161
EcoT38I GRGCYC 2 cut(s) 243, 285
ErhI CCWWGG 2 cut(s) 789, 1161
FaeI CATG 5 cut(s) 394, 422, 773, 1189, 1433
FalI AAGNNNNNCTT 2 cut(s) 563, 595
FaqI GGGAC 1 cut(s) 483
FatI CATG 5 cut(s) 390, 418, 769, 1185, 1429
FauI CCCGC 1 cut(s) 1104
FbaI TGATCA 2 cut(s) 778, 871
Fnu4HI GCNGC 3 cut(s) 103, 682, 828
FokI GGATG 3 cut(s) 135, 607, 986
FriOI GRGCYC 2 cut(s) 243, 285
Fsp4HI GCNGC 3 cut(s) 103, 682, 828
FspBI CTAG 4 cut(s) 615, 797, 950, 1329
GluI GCNGC 3 cut(s) 103, 682, 828
GsaI CCCAGC 1 cut(s) 1020
GsuI CTGGAG 1 cut(s) 940
HaeIII GGCC 2 cut(s) 102, 1022
HapII CCGG 2 cut(s) 99, 752
Hin1II CATG 5 cut(s) 394, 422, 773, 1189, 1433
HincII GTYRAC 2 cut(s) 469, 557
HindII GTYRAC 2 cut(s) 469, 557
HinfI GANTC 8 cut(s) 62, 182, 227, 456, 924, 978, 1189, 1244
HpaI GTTAAC 1 cut(s) 469
HpaII CCGG 2 cut(s) 99, 752
HphI GGTGA 7 cut(s) 72, 503, 578, 1127, 1187, 1229, 1388
Hpy166II GTNNAC 6 cut(s) 175, 469, 557, 1085, 1417, 1435
Hpy188III TCNNGA 3 cut(s) 596, 752, 985
Hpy8I GTNNAC 6 cut(s) 175, 469, 557, 1085, 1417, 1435
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 7 cut(s) 538, 585, 589, 819, 982, 1036, 1139
HpyCH4III ACNGT 6 cut(s) 52, 466, 497, 937, 1082, 1336
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 6 cut(s) 212, 453, 666, 827, 851, 1397
HpyF10VI GCNNNNNNNGC 5 cut(s) 690, 754, 1028, 1117, 1394
HpyF3I CTNAG 7 cut(s) 111, 138, 626, 639, 653, 930, 945
HpySE526I ACGT 1 cut(s) 123
Hsp92II CATG 5 cut(s) 394, 422, 773, 1189, 1433
Kpn2I TCCGGA 1 cut(s) 751
Ksp22I TGATCA 2 cut(s) 778, 871
KspAI GTTAAC 1 cut(s) 469
LmnI GCTCC 5 cut(s) 145, 343, 415, 754, 1048
Lsp1109I GCAGC 2 cut(s) 668, 839
LweI GCATC 5 cut(s) 157, 589, 1098, 1267, 1411
MaeI CTAG 4 cut(s) 615, 797, 950, 1329
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 4 cut(s) 646, 719, 1065, 1154
MbiI CCGCTC 1 cut(s) 338
MboII GAAGA 7 cut(s) 200, 203, 222, 894, 973, 1397, 1478
MflI RGATCY 5 cut(s) 193, 253, 319, 396, 1468
MhlI GDGCHC 3 cut(s) 243, 285, 1053
MluCI AATT 7 cut(s) 55, 76, 362, 609, 1203, 1287, 1366
MlyI GAGTC 3 cut(s) 236, 918, 1253
MroI TCCGGA 1 cut(s) 751
MseI TTAA 6 cut(s) 35, 381, 468, 492, 866, 1346
MspI CCGG 2 cut(s) 99, 752
MspR9I CCNGG 2 cut(s) 564, 919
Mva1269I GAATGC 1 cut(s) 1059
MvaI CCWGG 2 cut(s) 564, 919
MwoI GCNNNNNNNGC 5 cut(s) 690, 754, 1028, 1117, 1394
NlaIII CATG 5 cut(s) 394, 422, 773, 1189, 1433
NlaIV GGNNCC 2 cut(s) 756, 1021
NmuCI GTSAC 1 cut(s) 1065
NspI RCATGY 1 cut(s) 422
PaeI GCATGC 1 cut(s) 422
PctI GAATGC 1 cut(s) 1059
PfeI GAWTC 5 cut(s) 62, 182, 456, 978, 1189
PfoI TCCNGGA 1 cut(s) 917
PkrI GCNGC 3 cut(s) 104, 683, 829
PleI GAGTC 3 cut(s) 235, 918, 1252
PpsI GAGTC 3 cut(s) 235, 918, 1252
PpuMI RGGWCCY 1 cut(s) 915
PsiI TTATAA 1 cut(s) 1317
Psp124BI GAGCTC 2 cut(s) 243, 285
Psp5II RGGWCCY 1 cut(s) 915
Psp6I CCWGG 2 cut(s) 562, 917
PspFI CCCAGC 1 cut(s) 1016
PspGI CCWGG 2 cut(s) 562, 917
PspN4I GGNNCC 2 cut(s) 756, 1021
PspPI GGNCC 4 cut(s) 773, 915, 1020, 1085
PspPPI RGGWCCY 1 cut(s) 915
PsuI RGATCY 5 cut(s) 193, 253, 319, 396, 1468
RsaI GTAC 2 cut(s) 488, 1434
RsaNI GTAC 2 cut(s) 487, 1433
SacI GAGCTC 2 cut(s) 243, 285
SaqAI TTAA 6 cut(s) 35, 381, 468, 492, 866, 1346
SatI GCNGC 3 cut(s) 103, 682, 828
Sau96I GGNCC 4 cut(s) 773, 915, 1020, 1085
SchI GAGTC 3 cut(s) 236, 918, 1253
ScrFI CCNGG 2 cut(s) 564, 919
SduI GDGCHC 3 cut(s) 243, 285, 1053
SfaNI GCATC 5 cut(s) 157, 589, 1098, 1267, 1411
SfcI CTRYAG 1 cut(s) 342
SinI GGWCC 3 cut(s) 773, 915, 1085
SmlI CTYRAG 1 cut(s) 1193
SmoI CTYRAG 1 cut(s) 1193
SphI GCATGC 1 cut(s) 422
Sse9I AATT 7 cut(s) 55, 76, 362, 609, 1203, 1287, 1366
SsiI CCGC 6 cut(s) 86, 103, 336, 660, 1088, 1111
SspMI CTAG 4 cut(s) 615, 797, 950, 1329
SstI GAGCTC 2 cut(s) 243, 285
StyD4I CCNGG 2 cut(s) 562, 917
StyI CCWWGG 2 cut(s) 789, 1161
TaaI ACNGT 6 cut(s) 52, 466, 497, 937, 1082, 1336
TaiI ACGT 1 cut(s) 126
TaqI TCGA 2 cut(s) 591, 1247
TasI AATT 7 cut(s) 55, 76, 362, 609, 1203, 1287, 1366
TatI WGTACW 1 cut(s) 1432
TauI GCSGC 1 cut(s) 105
TfiI GAWTC 5 cut(s) 62, 182, 456, 978, 1189
Tru1I TTAA 6 cut(s) 35, 381, 468, 492, 866, 1346
Tru9I TTAA 6 cut(s) 35, 381, 468, 492, 866, 1346
TscAI CASTG 1 cut(s) 1087
TseFI GTSAC 1 cut(s) 1065
TseI GCWGC 2 cut(s) 681, 827
Tsp45I GTSAC 1 cut(s) 1065
TspDTI ATGAA 6 cut(s) 17, 375, 571, 1202, 1337, 1418
TspRI CASTG 1 cut(s) 1087
VpaK11BI GGWCC 3 cut(s) 773, 915, 1085
XapI RAATTY 4 cut(s) 55, 76, 362, 609
XceI RCATGY 1 cut(s) 422
XspI CTAG 4 cut(s) 615, 797, 950, 1329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.