Rh2BG155400

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0003684
Physical Location & Seq
Reverse (-)
13299486 .. 13303670
4185 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG155400.1

Sequence Viewer

Length: 1524 bp
ATGGCCGATGGGGATTCGGATTCTGACAGTAGCTATGTCCTTCTGAGTGATGGCAATTCTGTGGGTAGTGATTCTGAGAACTACTATGTCATTGTAGGTAAAGATGACTATATGAGTGCTGATTCCGGTGAAGATCTGACTGGTGGAAGCCAAAATGAGTTGGAGAAGGTTGTGGTTGATGAAAATCATGTGAGTTCTGACTCTGAAAATGATCAATTTTGTGATAGCCAACAACAGTTGGTGAAGAATGGAGATGATGACAGTAATGCGAGTACTGATTCTAATGAAGATCTGTCTGTTGAGAGAAGAAACCAGTTGAAGAAGCTTGTGGATTATTCCAGTTCTGATTCTGATAAAGATCTATTGGATTGTGTTGGATCACTGTGGAATGAAAGACGTGTGGCTACCAATGAGTTTCACAGATATCCATGTAAAAGAATTGGTCATGTTTGTGGAGAAGATTGCTATGAATTGTCTGGAGAGAAAAAGGCCGAGGACAATATTGACATCAATAATGTTACAATGGAGTATGTTCTTCCATTCCTCCCTGCCAAAACACTTTGCAGGTTCAGAAGCGTTGGCAAACAGTGGGATGAGTGGATTACCTCTCCTTTTTTCATTCACAAGCAGGCTTACTCCTTCCGGAACATCTCTGGTCTTTTCTGTCAACTTCCTGGGGAAAAGCCTTCTTTTGTCTCGCTCAATAGAGCTGCTTATGGAATTCCCAATCCTTCCCTTAATTTCTTGCCTGAACCAGTTACTGTAAGGACTGCTTGTAATGGCCTGCTCTGCTGCGAAAGCTGCGATGGAGAAAATACTTACTACATTTGCAATCCTGCGAACAAGGAATGGACAGTACTTCCCAAGGCAAAGCCTTATCATGGACATGAATCAGTTCTAGCACTAGCTTTTGAGCCTTCTGTGCTTGACTTTGAAGAACATTTTCAACTTGTGTGTGCCTTCTCTGTACCTGATGAGCCAGTGATCTGTTTTGATATATACTCTTCAAGTTCGAGGTCTTGGAAACTCTCTCAGACAGAATGCTGTGAGATTAATGCTCTGCATTTGAAAGGTCATGGGATCTTTTTGAAGGGAATGGTATTCTGGGAAACTCTTACTGGCGCAATTTTGGCTTTTAATCTGAAGGAGGAGCAATATGGTATTTTGCCTCTCCCTCCTCACAGCGGACCACTAGGTGTTATGGCAGAGATGTACGGCGAGCTGTGTTATATTCTGCCTGTTCTGGACTATGTATACACCTTGGAGATCTATGGTGATATGGACATGAAACTGAAGCGCAGAATTCCTCTTGCTGATGGTATTTTCACCAATGCTTATGAAGAGTTGAGGGCTTTGGCTTGTGTAACTGATGATGTGGTGATCATGCTTCTTGGAATGAACTTGATTGCTTATCATGTGAAAGCAGAGAAGGCTGATATCATACGTAGTTATACTGGGATTATTGCTGGATCTGCAGGTTATCTTCCATATGTGAACAGCCTGGTGCACATAAATCACCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

507

Amino Acids

56.63

Weight (kDa)

4.61

Isoelectric Point (pI)

44.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
b-prop_At3g26010-like PF24750 255 - 392 2.1e-07 F-box protein At3g26010-like, beta-propeller
FBA_3 PF08268 255 - 393 2.2e-06 F-box associated beta propeller domain
FBA_1 PF07734 257 - 395 2.4e-10 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 555, 1466
AccI GTMKAC 1 cut(s) 1254
AccIII TCCGGA 1 cut(s) 642
AciI CCGC 1 cut(s) 1185
AclWI GGATC 3 cut(s) 385, 1088, 1477
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 720, 1302
AcuI CTGAAG 2 cut(s) 1163, 1313
AdeI CACNNNGTG 1 cut(s) 1196
AfaI GTAC 4 cut(s) 274, 858, 969, 1214
AfiI CCNNNNNNNGG 2 cut(s) 881, 1184
AflIII ACRYGT 1 cut(s) 397
AgsI TTSAA 6 cut(s) 319, 935, 947, 1008, 1069, 1090
AjiI CACGTC 1 cut(s) 398
AjnI CCWGG 2 cut(s) 673, 1500
AluBI AGCT 6 cut(s) 33, 325, 710, 801, 908, 1222
AluI AGCT 6 cut(s) 33, 325, 710, 801, 908, 1222
Alw21I GWGCWC 1 cut(s) 1509
Alw26I GTCTC 1 cut(s) 700
Alw44I GTGCAC 1 cut(s) 1505
AlwI GGATC 3 cut(s) 385, 1088, 1477
AlwNI CAGNNNCTG 1 cut(s) 761
Aor13HI TCCGGA 1 cut(s) 642
AoxI GGCC 3 cut(s) 3, 489, 781
ApaLI GTGCAC 1 cut(s) 1505
ApeKI GCWGC 3 cut(s) 710, 792, 801
ApoI RAATTY 2 cut(s) 720, 1302
AseI ATTAAT 1 cut(s) 1053
Asp700I GAANNNNTTC 2 cut(s) 894, 942
AspLEI GCGC 2 cut(s) 1124, 1299
AspS9I GGNCC 1 cut(s) 1187
AsuHPI GGTGA 6 cut(s) 140, 253, 1286, 1318, 1390, 1508
AvaII GGWCC 1 cut(s) 1187
BaeGI GKGCMC 1 cut(s) 1509
Bbv12I GWGCWC 1 cut(s) 1509
BbvI GCAGC 3 cut(s) 697, 779, 788
BccI CCATC 4 cut(s) 2, 44, 800, 1310
BceAI ACGGC 1 cut(s) 1231
BciT130I CCWGG 2 cut(s) 675, 1502
BclI TGATCA 2 cut(s) 211, 1380
BcoDI GTCTC 1 cut(s) 700
BfaI CTAG 3 cut(s) 899, 905, 1193
BfmI CTRYAG 1 cut(s) 1473
BfuAI ACCTGC 2 cut(s) 555, 1466
BglII AGATCT 4 cut(s) 133, 289, 358, 1266
BisI GCNGC 3 cut(s) 711, 793, 802
BlsI GCNGC 3 cut(s) 712, 794, 803
BmcAI AGTACT 2 cut(s) 274, 858
Bme1390I CCNGG 2 cut(s) 675, 1502
Bme18I GGWCC 1 cut(s) 1187
BmgBI CACGTC 1 cut(s) 398
BmgT120I GGNCC 1 cut(s) 1187
BmrFI CCNGG 2 cut(s) 675, 1502
BmrI ACTGGG 1 cut(s) 1464
BmuI ACTGGG 1 cut(s) 1464
BpmI CTGGAG 1 cut(s) 498
BsaAI YACGTR 1 cut(s) 1445
BsaBI GATNNNNATC 2 cut(s) 183, 357
BsaJI CCNNGG 4 cut(s) 492, 674, 864, 1260
BsaWI WCCGGW 2 cut(s) 125, 642
Bsc4I CCNNNNNNNGG 2 cut(s) 881, 1184
Bse1I ACTGG 7 cut(s) 145, 313, 339, 755, 980, 1123, 1459
Bse8I GATNNNNATC 2 cut(s) 183, 357
BseAI TCCGGA 1 cut(s) 642
BseBI CCWGG 2 cut(s) 675, 1502
BseDI CCNNGG 4 cut(s) 492, 674, 864, 1260
BseGI GGATG 1 cut(s) 598
BseJI GATNNNNATC 2 cut(s) 183, 357
BseLI CCNNNNNNNGG 2 cut(s) 881, 1184
BseMII CTCAG 3 cut(s) 35, 66, 1046
BseNI ACTGG 7 cut(s) 145, 313, 339, 755, 980, 1123, 1459
BseRI GAGGAG 2 cut(s) 1163, 1167
BseSI GKGCMC 1 cut(s) 1509
BseXI GCAGC 3 cut(s) 697, 779, 788
BshFI GGCC 3 cut(s) 5, 491, 783
BsiHKAI GWGCWC 1 cut(s) 1509
BsiSI CCGG 2 cut(s) 126, 643
BslI CCNNNNNNNGG 2 cut(s) 881, 1184
BsmAI GTCTC 1 cut(s) 700
BsmI GAATGC 1 cut(s) 1046
BsnI GGCC 3 cut(s) 5, 491, 783
Bsp1286I GDGCHC 1 cut(s) 1509
Bsp13I TCCGGA 1 cut(s) 642
BspACI CCGC 1 cut(s) 1185
BspANI GGCC 3 cut(s) 5, 491, 783
BspCNI CTCAG 3 cut(s) 36, 67, 1045
BspEI TCCGGA 1 cut(s) 642
BspMAI CTGCAG 1 cut(s) 1477
BspMI ACCTGC 2 cut(s) 555, 1466
BspPI GGATC 3 cut(s) 385, 1088, 1477
BsrI ACTGG 7 cut(s) 145, 313, 339, 755, 980, 1123, 1459
BssECI CCNNGG 4 cut(s) 492, 674, 864, 1260
BssNAI GTATAC 1 cut(s) 1255
BssT1I CCWWGG 2 cut(s) 864, 1260
Bst1107I GTATAC 1 cut(s) 1255
Bst2UI CCWGG 2 cut(s) 675, 1502
Bst4CI ACNGT 7 cut(s) 29, 237, 263, 384, 588, 763, 856
Bst6I CTCTTC 2 cut(s) 1009, 1335
BstBAI YACGTR 1 cut(s) 1445
BstC8I GCNNGC 3 cut(s) 630, 785, 1220
BstDEI CTNAG 3 cut(s) 44, 75, 1032
BstF5I GGATG 1 cut(s) 598
BstHHI GCGC 2 cut(s) 1124, 1299
BstMAI GTCTC 1 cut(s) 700
BstMWI GCNNNNNNNGC 7 cut(s) 789, 798, 801, 922, 1130, 1430, 1472
BstNI CCWGG 2 cut(s) 675, 1502
BstSCI CCNGG 2 cut(s) 673, 1500
BstSFI CTRYAG 1 cut(s) 1473
BstSLI GKGCMC 1 cut(s) 1509
BstSNI TACGTA 1 cut(s) 1445
BstV1I GCAGC 3 cut(s) 697, 779, 788
BstX2I RGATCY 6 cut(s) 133, 289, 358, 1080, 1266, 1469
BstYI RGATCY 6 cut(s) 133, 289, 358, 1080, 1266, 1469
BstZ17I GTATAC 1 cut(s) 1255
BsuRI GGCC 3 cut(s) 5, 491, 783
BtgZI GCGATG 1 cut(s) 819
BtrI CACGTC 1 cut(s) 398
BtsCI GGATG 1 cut(s) 598
BtsIMutI CAGTG 3 cut(s) 380, 593, 987
BveI ACCTGC 2 cut(s) 555, 1466
Cac8I GCNNGC 3 cut(s) 630, 785, 1220
CaiI CAGNNNCTG 1 cut(s) 761
CfoI GCGC 2 cut(s) 1124, 1299
Cfr13I GGNCC 1 cut(s) 1187
Csp6I GTAC 4 cut(s) 273, 857, 968, 1213
CviQI GTAC 4 cut(s) 273, 857, 968, 1213
DdeI CTNAG 3 cut(s) 44, 75, 1032
DraIII CACNNNGTG 1 cut(s) 1196
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 1009, 1335
EarI CTCTTC 2 cut(s) 1009, 1335
Eco105I TACGTA 1 cut(s) 1445
Eco130I CCWWGG 2 cut(s) 864, 1260
Eco32I GATATC 2 cut(s) 425, 1438
Eco47I GGWCC 1 cut(s) 1187
Eco57I CTGAAG 2 cut(s) 1163, 1313
EcoRI GAATTC 2 cut(s) 720, 1302
EcoRII CCWGG 2 cut(s) 673, 1500
EcoRV GATATC 2 cut(s) 425, 1438
EcoT14I CCWWGG 2 cut(s) 864, 1260
ErhI CCWWGG 2 cut(s) 864, 1260
FalI AAGNNNNNCTT 2 cut(s) 757, 789
FauNDI CATATG 1 cut(s) 1489
FbaI TGATCA 2 cut(s) 211, 1380
FblI GTMKAC 1 cut(s) 1254
Fnu4HI GCNGC 3 cut(s) 711, 793, 802
FokI GGATG 1 cut(s) 605
Fsp4HI GCNGC 3 cut(s) 711, 793, 802
FspBI CTAG 3 cut(s) 899, 905, 1193
GlaI GCGC 2 cut(s) 1123, 1298
GluI GCNGC 3 cut(s) 711, 793, 802
GsuI CTGGAG 1 cut(s) 498
HaeIII GGCC 3 cut(s) 5, 491, 783
HapII CCGG 2 cut(s) 126, 643
HhaI GCGC 2 cut(s) 1124, 1299
Hin6I GCGC 2 cut(s) 1122, 1297
HinP1I GCGC 2 cut(s) 1122, 1297
HincII GTYRAC 1 cut(s) 668
HindII GTYRAC 1 cut(s) 668
HindIII AAGCTT 1 cut(s) 323
HinfI GANTC 8 cut(s) 14, 20, 71, 122, 200, 278, 347, 890
HpaII CCGG 2 cut(s) 126, 643
HphI GGTGA 6 cut(s) 140, 253, 1286, 1318, 1390, 1508
Hpy166II GTNNAC 4 cut(s) 668, 1255, 1495, 1507
Hpy188III TCNNGA 3 cut(s) 477, 643, 1244
Hpy8I GTNNAC 4 cut(s) 668, 1255, 1495, 1507
HpyCH4III ACNGT 7 cut(s) 29, 237, 263, 384, 588, 763, 856
HpyCH4IV ACGT 2 cut(s) 397, 1444
HpyCH4V TGCA 5 cut(s) 564, 831, 1063, 1475, 1507
HpyF10VI GCNNNNNNNGC 7 cut(s) 789, 798, 801, 922, 1130, 1430, 1472
HpyF3I CTNAG 3 cut(s) 44, 75, 1032
HpySE526I ACGT 2 cut(s) 397, 1444
HspAI GCGC 2 cut(s) 1122, 1297
Kpn2I TCCGGA 1 cut(s) 642
Ksp22I TGATCA 2 cut(s) 211, 1380
LmnI GCTCC 1 cut(s) 1150
Lsp1109I GCAGC 3 cut(s) 697, 779, 788
MaeI CTAG 3 cut(s) 899, 905, 1193
MaeII ACGT 2 cut(s) 397, 1444
MaeIII GTNAC 3 cut(s) 517, 757, 1363
MflI RGATCY 6 cut(s) 133, 289, 358, 1080, 1266, 1469
MhlI GDGCHC 1 cut(s) 1509
MluCI AATT 8 cut(s) 55, 215, 438, 470, 720, 739, 1125, 1302
MlyI GAGTC 1 cut(s) 194
MmeI TCCRAC 2 cut(s) 141, 355
MroI TCCGGA 1 cut(s) 642
MroXI GAANNNNTTC 2 cut(s) 894, 942
MseI TTAA 3 cut(s) 738, 1053, 1137
MslI CAYNNNNRTG 2 cut(s) 450, 885
MspA1I CMGCKG 1 cut(s) 1185
MspI CCGG 2 cut(s) 126, 643
MspR9I CCNGG 2 cut(s) 675, 1502
Mva1269I GAATGC 1 cut(s) 1046
MvaI CCWGG 2 cut(s) 675, 1502
MwoI GCNNNNNNNGC 7 cut(s) 789, 798, 801, 922, 1130, 1430, 1472
NdeI CATATG 1 cut(s) 1489
NmeAIII GCCGAG 1 cut(s) 517
PctI GAATGC 1 cut(s) 1046
PdmI GAANNNNTTC 2 cut(s) 894, 942
PfeI GAWTC 7 cut(s) 14, 20, 71, 122, 278, 347, 890
PkrI GCNGC 3 cut(s) 712, 794, 803
PleI GAGTC 1 cut(s) 194
PpsI GAGTC 1 cut(s) 194
Ppu21I YACGTR 1 cut(s) 1445
PshBI ATTAAT 1 cut(s) 1053
Psp6I CCWGG 2 cut(s) 673, 1500
PspGI CCWGG 2 cut(s) 673, 1500
PspPI GGNCC 1 cut(s) 1187
PstI CTGCAG 1 cut(s) 1477
PstNI CAGNNNCTG 1 cut(s) 761
PsuI RGATCY 6 cut(s) 133, 289, 358, 1080, 1266, 1469
RsaI GTAC 4 cut(s) 274, 858, 969, 1214
RsaNI GTAC 4 cut(s) 273, 857, 968, 1213
RseI CAYNNNNRTG 2 cut(s) 450, 885
SaqAI TTAA 3 cut(s) 738, 1053, 1137
SatI GCNGC 3 cut(s) 711, 793, 802
Sau96I GGNCC 1 cut(s) 1187
ScaI AGTACT 2 cut(s) 274, 858
SchI GAGTC 1 cut(s) 194
ScrFI CCNGG 2 cut(s) 675, 1502
SduI GDGCHC 1 cut(s) 1509
SfcI CTRYAG 1 cut(s) 1473
SinI GGWCC 1 cut(s) 1187
SmiMI CAYNNNNRTG 2 cut(s) 450, 885
SnaBI TACGTA 1 cut(s) 1445
Sse9I AATT 8 cut(s) 55, 215, 438, 470, 720, 739, 1125, 1302
SsiI CCGC 1 cut(s) 1185
SspI AATATT 1 cut(s) 502
SspMI CTAG 3 cut(s) 899, 905, 1193
StyD4I CCNGG 2 cut(s) 673, 1500
StyI CCWWGG 2 cut(s) 864, 1260
TaaI ACNGT 7 cut(s) 29, 237, 263, 384, 588, 763, 856
TaiI ACGT 2 cut(s) 400, 1447
TaqI TCGA 1 cut(s) 1013
TasI AATT 8 cut(s) 55, 215, 438, 470, 720, 739, 1125, 1302
TatI WGTACW 2 cut(s) 272, 856
TfiI GAWTC 7 cut(s) 14, 20, 71, 122, 278, 347, 890
Tru1I TTAA 3 cut(s) 738, 1053, 1137
Tru9I TTAA 3 cut(s) 738, 1053, 1137
TscAI CASTG 3 cut(s) 387, 593, 987
TseI GCWGC 3 cut(s) 710, 792, 801
TspDTI ATGAA 9 cut(s) 195, 300, 405, 483, 607, 903, 1301, 1353, 1412
TspRI CASTG 3 cut(s) 387, 593, 987
VneI GTGCAC 1 cut(s) 1505
VpaK11BI GGWCC 1 cut(s) 1187
VspI ATTAAT 1 cut(s) 1053
XapI RAATTY 2 cut(s) 720, 1302
XmiI GTMKAC 1 cut(s) 1254
XmnI GAANNNNTTC 2 cut(s) 894, 942
XspI CTAG 3 cut(s) 899, 905, 1193
ZrmI AGTACT 2 cut(s) 274, 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.