Rroxscaffold_2G00140990

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003684
Physical Location & Seq
Forward (+)
79319487 .. 79322511
3025 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00140990.1

Sequence Viewer

Length: 1524 bp
ATGGCCGATGGGGATTCGGATTCTGACAGTAGCTATGTCCTTCTGAGTGATGGCAATTCTGTGGGTAGTGATTCTGAGAACTATGTCATTGTGGGTAAAGACGGCTATATGAGTGCTGATTCTGGTGAAGATCTGACTGGTGGAAGCCAAAATGAGTTGGAGAAGGTTGTGGTTGATGAAAATCATGTGAGTTCTGACTCTGAAAATGATCAATTTTCTGATAGCCAACAACAGTTGGAGAAGAATGGAGATGATGACAGTAATGCGAGTACTGATTCTGATGAAGATCTGTCTGTTGAGAGAAGAAACCAGTGGAAGAAGCTTGTGGATTACTCCAGTTCTGATTCTGATAAAGATCTATTGGATTGTGGTGGATCACTGTGGAATGAAAGACGTTTGGCTACCAATGAGTTTCACAGATATCCATGTAAAAGAATTGGTCATGTTTGTGGAGAAGATTGCTATGAATTGTCTGGAGAGAAAAAGGCCAAGGACAATATTGACATCAATAATGTTACAATGGAGTATGTTCTTCCATTCCTCCCTGCCAAAACACTTTGCAGGTTCAGAAGCGTTGGCAAACAGTGGGATGAGTGGATTACCTCTCCTTTTTTCATTCACAAGCAGGCTTACTCCTTCCGGAACATCTCTGGTCTTTTCTGTCAACTTCCTGGGGAAAAGCCTTCTTTTGTCTCGCTCAATAGAGCTGCGTATGGAATTCCCAATCCTTCCCTTAATTTCTTGCCTGAACCAGTTACTGTAAGGACTGCTTGTAATGGCCTGCTCTGCTGCGAAAGCTGCGATGGAGAAAATACTTACTACATTTGCAATCCTGTGAACAAGGAATGGACAGTGCTTCCCAAGTCAAAGCCTTATCATGGACATGAATCAGTTCTAGCACTAGCTTTTGAGCCTTCTGTGCTTGACTTTGAAGGACATTTTCACCTTGTGTGTGCCTTCTCTGTACCTGATGAGCCAGTGATCTGTTTTGATATATACTCTTCAAGGTCGAGGTCTTGGAAACTCTCTCAGACAGAATGCTGTGAGATTGATGCTCTGCATTTGAAAGGTCATGGGATCTTTTTGAAGGGAATGGTATTCTGGGAAACTCTTACCGGCGCAATTTTGGCTTTTAATCTGAAGGAGGAGCAATATGGTATTTTGCCTCTCCCTCCTCACAGCGGACCACTAGGTGTTATGGCAGAGCTGTACGGCGAGCTGTGTTATATTCTGCCTGTTCTGGAAGACTATGTGTACACCTTGGAGATCTATGGTGATATGGACATGAAACTGAAGCGCAGAATTCCTCTTGCTGATGGTATTTTCACCAATGCTTATGAAGAGTTGAGGGCTTTGGCTTGTGTAACTGATGATGTGGTGATCATGCTCCTTGGAATGCGATTGATTGCTTATCATGTGAAAGCAGAGAAGGCTGATATCATACATAGTTATGCTGGGATTATTGCTGGATATGCCGGCTATCTTCCATATGTGAACAGCCTGGTGCACATAAATCACCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

507

Amino Acids

56.7

Weight (kDa)

4.7

Isoelectric Point (pI)

43.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
b-prop_At3g26010-like PF24750 254 - 391 3.3e-08 F-box protein At3g26010-like, beta-propeller
FBA_3 PF08268 254 - 392 1.3e-06 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 552
AccIII TCCGGA 1 cut(s) 639
AciI CCGC 1 cut(s) 1182
AclWI GGATC 2 cut(s) 382, 1085
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 717, 1302
AcuI CTGAAG 2 cut(s) 1160, 1313
AdeI CACNNNGTG 2 cut(s) 949, 1193
AfaI GTAC 4 cut(s) 271, 966, 1211, 1256
AfiI CCNNNNNNNGG 2 cut(s) 878, 1181
AgsI TTSAA 4 cut(s) 932, 1005, 1066, 1087
AjnI CCWGG 2 cut(s) 670, 1500
AluBI AGCT 7 cut(s) 33, 322, 707, 798, 905, 1207, 1219
AluI AGCT 7 cut(s) 33, 322, 707, 798, 905, 1207, 1219
Alw21I GWGCWC 1 cut(s) 1509
Alw26I GTCTC 1 cut(s) 697
Alw44I GTGCAC 1 cut(s) 1505
AlwI GGATC 2 cut(s) 382, 1085
AlwNI CAGNNNCTG 1 cut(s) 758
Aor13HI TCCGGA 1 cut(s) 639
AoxI GGCC 3 cut(s) 3, 486, 778
ApaLI GTGCAC 1 cut(s) 1505
ApeKI GCWGC 3 cut(s) 707, 789, 798
ApoI RAATTY 2 cut(s) 717, 1302
Asp700I GAANNNNTTC 1 cut(s) 891
AspLEI GCGC 2 cut(s) 1121, 1299
AspS9I GGNCC 1 cut(s) 1184
AsuHPI GGTGA 6 cut(s) 137, 935, 1286, 1318, 1390, 1508
AvaII GGWCC 1 cut(s) 1184
BaeGI GKGCMC 1 cut(s) 1509
BbsI GAAGAC 1 cut(s) 1251
Bbv12I GWGCWC 1 cut(s) 1509
BbvI GCAGC 3 cut(s) 694, 776, 785
BccI CCATC 4 cut(s) 2, 44, 797, 1310
BceAI ACGGC 2 cut(s) 118, 1228
BciT130I CCWGG 2 cut(s) 672, 1502
BclI TGATCA 2 cut(s) 208, 1380
BcoDI GTCTC 1 cut(s) 697
BfaI CTAG 3 cut(s) 896, 902, 1190
BfuAI ACCTGC 1 cut(s) 552
BglII AGATCT 4 cut(s) 130, 286, 355, 1266
BisI GCNGC 3 cut(s) 708, 790, 799
BlsI GCNGC 3 cut(s) 709, 791, 800
BmcAI AGTACT 1 cut(s) 271
Bme1390I CCNGG 2 cut(s) 672, 1502
Bme18I GGWCC 1 cut(s) 1184
BmgT120I GGNCC 1 cut(s) 1184
BmrFI CCNGG 2 cut(s) 672, 1502
BmsI GCATC 1 cut(s) 1042
BpiI GAAGAC 1 cut(s) 1251
BpmI CTGGAG 2 cut(s) 319, 495
BsaBI GATNNNNATC 3 cut(s) 180, 285, 354
BsaJI CCNNGG 4 cut(s) 489, 671, 1260, 1390
BsaWI WCCGGW 1 cut(s) 639
Bsc4I CCNNNNNNNGG 2 cut(s) 878, 1181
Bse118I RCCGGY 2 cut(s) 1115, 1475
Bse1I ACTGG 5 cut(s) 142, 310, 336, 752, 977
Bse8I GATNNNNATC 3 cut(s) 180, 285, 354
BseAI TCCGGA 1 cut(s) 639
BseBI CCWGG 2 cut(s) 672, 1502
BseDI CCNNGG 4 cut(s) 489, 671, 1260, 1390
BseGI GGATG 1 cut(s) 595
BseJI GATNNNNATC 3 cut(s) 180, 285, 354
BseLI CCNNNNNNNGG 2 cut(s) 878, 1181
BseMII CTCAG 3 cut(s) 35, 66, 1043
BseNI ACTGG 5 cut(s) 142, 310, 336, 752, 977
BseRI GAGGAG 2 cut(s) 1160, 1164
BseSI GKGCMC 1 cut(s) 1509
BseXI GCAGC 3 cut(s) 694, 776, 785
BseYI CCCAGC 1 cut(s) 1454
BshFI GGCC 3 cut(s) 5, 488, 780
BsiHKAI GWGCWC 1 cut(s) 1509
BsiSI CCGG 3 cut(s) 640, 1116, 1476
BslI CCNNNNNNNGG 2 cut(s) 878, 1181
BsmAI GTCTC 1 cut(s) 697
BsmI GAATGC 2 cut(s) 1043, 1401
BsnI GGCC 3 cut(s) 5, 488, 780
Bsp1286I GDGCHC 1 cut(s) 1509
Bsp13I TCCGGA 1 cut(s) 639
Bsp1407I TGTACA 1 cut(s) 1254
Bsp143I GATC 9 cut(s) 130, 208, 286, 355, 374, 981, 1077, 1266, 1380
BspACI CCGC 1 cut(s) 1182
BspANI GGCC 3 cut(s) 5, 488, 780
BspCNI CTCAG 3 cut(s) 36, 67, 1042
BspEI TCCGGA 1 cut(s) 639
BspMI ACCTGC 1 cut(s) 552
BspPI GGATC 2 cut(s) 382, 1085
BsrFI RCCGGY 2 cut(s) 1115, 1475
BsrGI TGTACA 1 cut(s) 1254
BsrI ACTGG 5 cut(s) 142, 310, 336, 752, 977
BssAI RCCGGY 2 cut(s) 1115, 1475
BssECI CCNNGG 4 cut(s) 489, 671, 1260, 1390
BssMI GATC 9 cut(s) 130, 208, 286, 355, 374, 981, 1077, 1266, 1380
BssT1I CCWWGG 3 cut(s) 489, 1260, 1390
Bst2UI CCWGG 2 cut(s) 672, 1502
Bst4CI ACNGT 7 cut(s) 29, 234, 260, 381, 585, 760, 853
Bst6I CTCTTC 2 cut(s) 1006, 1335
BstAUI TGTACA 1 cut(s) 1254
BstC8I GCNNGC 4 cut(s) 627, 782, 1217, 1477
BstDEI CTNAG 3 cut(s) 44, 75, 1029
BstF5I GGATG 1 cut(s) 595
BstHHI GCGC 2 cut(s) 1121, 1299
BstKTI GATC 9 cut(s) 133, 211, 289, 358, 377, 984, 1080, 1269, 1383
BstMAI GTCTC 1 cut(s) 697
BstMBI GATC 9 cut(s) 130, 208, 286, 355, 374, 981, 1077, 1266, 1380
BstMWI GCNNNNNNNGC 7 cut(s) 786, 795, 798, 919, 1127, 1430, 1472
BstNI CCWGG 2 cut(s) 672, 1502
BstSCI CCNGG 2 cut(s) 670, 1500
BstSLI GKGCMC 1 cut(s) 1509
BstV1I GCAGC 3 cut(s) 694, 776, 785
BstV2I GAAGAC 1 cut(s) 1251
BstX2I RGATCY 5 cut(s) 130, 286, 355, 1077, 1266
BstYI RGATCY 5 cut(s) 130, 286, 355, 1077, 1266
BsuRI GGCC 3 cut(s) 5, 488, 780
BtgZI GCGATG 1 cut(s) 816
BtsCI GGATG 1 cut(s) 595
BtsIMutI CAGTG 5 cut(s) 317, 377, 590, 858, 984
BveI ACCTGC 1 cut(s) 552
Cac8I GCNNGC 4 cut(s) 627, 782, 1217, 1477
CaiI CAGNNNCTG 1 cut(s) 758
CfoI GCGC 2 cut(s) 1121, 1299
Cfr10I RCCGGY 2 cut(s) 1115, 1475
Cfr13I GGNCC 1 cut(s) 1184
Csp6I GTAC 4 cut(s) 270, 965, 1210, 1255
CviQI GTAC 4 cut(s) 270, 965, 1210, 1255
DdeI CTNAG 3 cut(s) 44, 75, 1029
DpnI GATC 9 cut(s) 132, 210, 288, 357, 376, 983, 1079, 1268, 1382
DpnII GATC 9 cut(s) 130, 208, 286, 355, 374, 981, 1077, 1266, 1380
DraIII CACNNNGTG 2 cut(s) 949, 1193
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 1006, 1335
EarI CTCTTC 2 cut(s) 1006, 1335
Eco130I CCWWGG 3 cut(s) 489, 1260, 1390
Eco32I GATATC 2 cut(s) 422, 1438
Eco47I GGWCC 1 cut(s) 1184
Eco57I CTGAAG 2 cut(s) 1160, 1313
EcoRI GAATTC 2 cut(s) 717, 1302
EcoRII CCWGG 2 cut(s) 670, 1500
EcoRV GATATC 2 cut(s) 422, 1438
EcoT14I CCWWGG 3 cut(s) 489, 1260, 1390
ErhI CCWWGG 3 cut(s) 489, 1260, 1390
FalI AAGNNNNNCTT 2 cut(s) 754, 786
FauNDI CATATG 1 cut(s) 1489
FbaI TGATCA 2 cut(s) 208, 1380
Fnu4HI GCNGC 3 cut(s) 708, 790, 799
FokI GGATG 1 cut(s) 602
Fsp4HI GCNGC 3 cut(s) 708, 790, 799
FspBI CTAG 3 cut(s) 896, 902, 1190
GlaI GCGC 2 cut(s) 1120, 1298
GluI GCNGC 3 cut(s) 708, 790, 799
GsaI CCCAGC 1 cut(s) 1458
GsuI CTGGAG 2 cut(s) 319, 495
HaeIII GGCC 3 cut(s) 5, 488, 780
HapII CCGG 3 cut(s) 640, 1116, 1476
HhaI GCGC 2 cut(s) 1121, 1299
Hin6I GCGC 2 cut(s) 1119, 1297
HinP1I GCGC 2 cut(s) 1119, 1297
HincII GTYRAC 1 cut(s) 665
HindII GTYRAC 1 cut(s) 665
HindIII AAGCTT 1 cut(s) 320
HinfI GANTC 8 cut(s) 14, 20, 71, 119, 197, 275, 344, 887
HpaII CCGG 3 cut(s) 640, 1116, 1476
HphI GGTGA 6 cut(s) 137, 935, 1286, 1318, 1390, 1508
Hpy166II GTNNAC 6 cut(s) 665, 838, 1255, 1257, 1495, 1507
Hpy188III TCNNGA 3 cut(s) 474, 640, 1241
Hpy8I GTNNAC 6 cut(s) 665, 838, 1255, 1257, 1495, 1507
HpyCH4III ACNGT 7 cut(s) 29, 234, 260, 381, 585, 760, 853
HpyCH4IV ACGT 1 cut(s) 394
HpyCH4V TGCA 4 cut(s) 561, 828, 1060, 1507
HpyF10VI GCNNNNNNNGC 7 cut(s) 786, 795, 798, 919, 1127, 1430, 1472
HpyF3I CTNAG 3 cut(s) 44, 75, 1029
HpySE526I ACGT 1 cut(s) 394
HspAI GCGC 2 cut(s) 1119, 1297
Kpn2I TCCGGA 1 cut(s) 639
KroI GCCGGC 1 cut(s) 1475
KroNI GCCGGC 1 cut(s) 1477
Ksp22I TGATCA 2 cut(s) 208, 1380
Kzo9I GATC 9 cut(s) 130, 208, 286, 355, 374, 981, 1077, 1266, 1380
LmnI GCTCC 2 cut(s) 1147, 1392
Lsp1109I GCAGC 3 cut(s) 694, 776, 785
LweI GCATC 1 cut(s) 1042
MaeI CTAG 3 cut(s) 896, 902, 1190
MaeII ACGT 1 cut(s) 394
MaeIII GTNAC 3 cut(s) 514, 754, 1363
MalI GATC 9 cut(s) 132, 210, 288, 357, 376, 983, 1079, 1268, 1382
MboI GATC 9 cut(s) 130, 208, 286, 355, 374, 981, 1077, 1266, 1380
MflI RGATCY 5 cut(s) 130, 286, 355, 1077, 1266
MhlI GDGCHC 1 cut(s) 1509
MluCI AATT 8 cut(s) 55, 212, 435, 467, 717, 736, 1122, 1302
MlyI GAGTC 1 cut(s) 191
MmeI TCCRAC 2 cut(s) 138, 216
MnlI CCTC 9 cut(s) 551, 613, 1005, 1138, 1176, 1182, 1185, 1317, 1341
MroI TCCGGA 1 cut(s) 639
MroNI GCCGGC 1 cut(s) 1475
MroXI GAANNNNTTC 1 cut(s) 891
MseI TTAA 2 cut(s) 735, 1134
MslI CAYNNNNRTG 3 cut(s) 447, 882, 1449
MspA1I CMGCKG 1 cut(s) 1182
MspI CCGG 3 cut(s) 640, 1116, 1476
MspR9I CCNGG 2 cut(s) 672, 1502
Mva1269I GAATGC 2 cut(s) 1043, 1401
MvaI CCWGG 2 cut(s) 672, 1502
MwoI GCNNNNNNNGC 7 cut(s) 786, 795, 798, 919, 1127, 1430, 1472
NaeI GCCGGC 1 cut(s) 1477
NdeI CATATG 1 cut(s) 1489
NdeII GATC 9 cut(s) 130, 208, 286, 355, 374, 981, 1077, 1266, 1380
NgoMIV GCCGGC 1 cut(s) 1475
PctI GAATGC 2 cut(s) 1043, 1401
PdiI GCCGGC 1 cut(s) 1477
PdmI GAANNNNTTC 1 cut(s) 891
PfeI GAWTC 7 cut(s) 14, 20, 71, 119, 275, 344, 887
PkrI GCNGC 3 cut(s) 709, 791, 800
PleI GAGTC 1 cut(s) 191
PpsI GAGTC 1 cut(s) 191
Psp6I CCWGG 2 cut(s) 670, 1500
PspFI CCCAGC 1 cut(s) 1454
PspGI CCWGG 2 cut(s) 670, 1500
PspPI GGNCC 1 cut(s) 1184
PstNI CAGNNNCTG 1 cut(s) 758
PsuI RGATCY 5 cut(s) 130, 286, 355, 1077, 1266
RsaI GTAC 4 cut(s) 271, 966, 1211, 1256
RsaNI GTAC 4 cut(s) 270, 965, 1210, 1255
RseI CAYNNNNRTG 3 cut(s) 447, 882, 1449
SaqAI TTAA 2 cut(s) 735, 1134
SatI GCNGC 3 cut(s) 708, 790, 799
Sau3AI GATC 9 cut(s) 130, 208, 286, 355, 374, 981, 1077, 1266, 1380
Sau96I GGNCC 1 cut(s) 1184
ScaI AGTACT 1 cut(s) 271
SchI GAGTC 1 cut(s) 191
ScrFI CCNGG 2 cut(s) 672, 1502
SduI GDGCHC 1 cut(s) 1509
SfaNI GCATC 1 cut(s) 1042
SinI GGWCC 1 cut(s) 1184
SmiMI CAYNNNNRTG 3 cut(s) 447, 882, 1449
Sse9I AATT 8 cut(s) 55, 212, 435, 467, 717, 736, 1122, 1302
SsiI CCGC 1 cut(s) 1182
SspI AATATT 1 cut(s) 499
SspMI CTAG 3 cut(s) 896, 902, 1190
StyD4I CCNGG 2 cut(s) 670, 1500
StyI CCWWGG 3 cut(s) 489, 1260, 1390
TaaI ACNGT 7 cut(s) 29, 234, 260, 381, 585, 760, 853
TaiI ACGT 1 cut(s) 397
TaqI TCGA 1 cut(s) 1010
TasI AATT 8 cut(s) 55, 212, 435, 467, 717, 736, 1122, 1302
TatI WGTACW 2 cut(s) 269, 1254
TfiI GAWTC 7 cut(s) 14, 20, 71, 119, 275, 344, 887
Tru1I TTAA 2 cut(s) 735, 1134
Tru9I TTAA 2 cut(s) 735, 1134
TscAI CASTG 5 cut(s) 317, 384, 590, 858, 984
TseI GCWGC 3 cut(s) 707, 789, 798
TspDTI ATGAA 8 cut(s) 192, 297, 402, 480, 604, 900, 1301, 1353
TspRI CASTG 5 cut(s) 317, 384, 590, 858, 984
VneI GTGCAC 1 cut(s) 1505
VpaK11BI GGWCC 1 cut(s) 1184
XapI RAATTY 2 cut(s) 717, 1302
XmnI GAANNNNTTC 1 cut(s) 891
XspI CTAG 3 cut(s) 896, 902, 1190
ZrmI AGTACT 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.