pycom15g23040

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003684
Physical Location & Seq
Reverse (-)
17108265 .. 17110233
1969 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g23040.1

Sequence Viewer

Length: 1608 bp
ATGGATAGCGATAAAGATGTTGGCAATGACTCTGATTCTTTTGTCATTGTGAGCAATGCCAATTCTGTGTGCAGTGATTCTGAAAGTGTCGTGGGTACTGATTCTGAAAATTATGTCATTGTGAGTCCTCAAAACAATGTAGCTACAGATTCCGGAGAAGATCTATTTGTTGGAAGCACACAACAGTCGAAGAATGTGGAAAAGGACGCGTATGTGTGTTCTGACTCTGACTCTGGTGAGAGCACTTATTCTGAAGAAGTTCTATCTGATGAAAGTGAACAACATTTGAAGGAAGCGGATAATGCTAGTTATCTGAGTACCGATTCTGAAGTCTTTCTATTTGACGAAAGCGAACAGCAGTTGAAGAATGAGGATATGAGTACTGATTCTGTAGAAGAACTATCTGATGGATTCGGAAAACAGTTGAAGATTATGGATAATCAACATTACATGGATATTGTACTCCCTGGGAAACAGTTGAAGACGAGGAATTGTTCTTGTGAGTTTTACACATATCCATGCAAAAGAATCCGTCGTGCACATGATTGTTCTGAACTTCCCCGTGACGAAAGGCTTTATAATAAGAAAATGGACATCAGTGATGTTGCAATGGAGAATGTTCTTCCTTTCCTCCCTGCGAAAATGTTGTGCAGGTTGAGAACTGTTAGCAGACTCTGGGATAAGTGGATTTTTAGTCCTTTCTTCAGTCACAAGCAAACTTACTACTTCAGGGAAATCTCCGGGCTTTTTTGTCAAGCCCCCGGTGGAAACCCTTACTTCCTCTCACTTGATAATGATGCTTACGGCATTCCCAGATCTTACCTCAGCTTCTTGCCCGAACCAGTTACTGTAAAGGCCACTTGCAATGGTCTGGTTTGCTGCCAGAGCGAAGACAGAGAAGATACCTACTACATTTGCAACCCTGTGAACATGAGATTTAGAAAGCTTCCCAAGTCAGGTTTATATCATGGGCCTGAGTCTTACGCGGCACTAGCCTTTGAGCCTTCTGTGCTTGACTTTGAAGTGTGTTTTCAACTTGTATGTGCCTTCTCTGTACCTGGTCATCCAGGTGTCCATTTTGATATGTACTCTTCAAGGTCAAGTGGTTGGGAACTCGCTGAGACGGTATGCTATGAACTGGAGACTACGGAGTTGGAAGGTTATACCCTCTATCTAAAGGGTGTACTCTTTTGGGAAACTTTAACTGGGGTCATTTTGGCTTTCGATGTGAAGGAGCAGCAATATGGTATTCTGCCACTCCCCTTCAGCACAGGACCACGCGGTGTTATTTCACAGATGAATGGAGAGCTGTGTTACATTTTGCCTCGTGTTGAATTCGGTCAGTTCTCCTTGGAGATTTACGGTAATATGGACATGAGCCTGAAGCAGAAAATTTATCTCGTCGGCCAGATTTTCACCTCTTTTGTGGACACAGTTAGGTTATTGGCTTGTGTGAATGATGATGTGATGCTAATTCTTGTCGGACAGAGGGTGATCGTGTATGGTGTGAAAGCCGGGGAGGAAAAAGTTTTGAAAATACTTGACTACGGCCTTGGTGCTGGTAATGAAAGGTATCTTCCATATGTGAACAGCCTTGTGCAGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

536

Amino Acids

60.37

Weight (kDa)

4.55

Isoelectric Point (pI)

43.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
b-prop_At3g26010-like PF24750 270 - 439 6.1e-07 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 579
Acc36I ACCTGC 1 cut(s) 642
AccII CGCG 3 cut(s) 209, 986, 1281
AccIII TCCGGA 1 cut(s) 152
AciI CCGC 3 cut(s) 296, 986, 1281
AcoI YGGCCR 1 cut(s) 1405
AcsI RAATTY 2 cut(s) 1334, 1392
AcuI CTGAAG 6 cut(s) 273, 348, 688, 712, 1249, 1403
AdeI CACNNNGTG 1 cut(s) 1283
AfaI GTAC 7 cut(s) 97, 319, 382, 462, 1056, 1088, 1185
AfiI CCNNNNNNNGG 1 cut(s) 956
AflIII ACRYGT 1 cut(s) 207
AgsI TTSAA 9 cut(s) 289, 364, 427, 481, 1022, 1034, 1095, 1334, 1534
AjnI CCWGG 3 cut(s) 466, 1057, 1066
AluBI AGCT 4 cut(s) 143, 828, 946, 1309
AluI AGCT 4 cut(s) 143, 828, 946, 1309
Alw21I GWGCWC 2 cut(s) 245, 541
Alw26I GTCTC 2 cut(s) 1115, 1136
Alw44I GTGCAC 1 cut(s) 537
AlwNI CAGNNNCTG 2 cut(s) 675, 848
Aor13HI TCCGGA 1 cut(s) 152
AoxI GGCC 4 cut(s) 855, 971, 1405, 1549
ApaLI GTGCAC 1 cut(s) 537
ApeKI GCWGC 2 cut(s) 879, 1237
ApoI RAATTY 2 cut(s) 1334, 1392
Asp700I GAANNNNTTC 2 cut(s) 258, 333
AspS9I GGNCC 2 cut(s) 971, 1274
AsuC2I CCSGG 3 cut(s) 742, 762, 1516
AsuHPI GGTGA 3 cut(s) 248, 1408, 1504
AvaII GGWCC 1 cut(s) 1274
BaeGI GKGCMC 1 cut(s) 541
BauI CACGAG 1 cut(s) 1326
BbsI GAAGAC 2 cut(s) 488, 897
Bbv12I GWGCWC 2 cut(s) 245, 541
BbvCI CCTCAGC 1 cut(s) 824
BbvI GCAGC 2 cut(s) 866, 1249
BccI CCATC 1 cut(s) 401
BceAI ACGGC 2 cut(s) 820, 1564
BciT130I CCWGG 3 cut(s) 468, 1059, 1068
BcnI CCSGG 3 cut(s) 742, 762, 1516
BcoDI GTCTC 2 cut(s) 1115, 1136
BfaI CTAG 3 cut(s) 306, 992, 1606
BfmI CTRYAG 2 cut(s) 144, 390
BfuAI ACCTGC 1 cut(s) 642
BglII AGATCT 3 cut(s) 160, 815, 1602
BisI GCNGC 3 cut(s) 880, 987, 1238
BlsI GCNGC 3 cut(s) 881, 988, 1239
BmcAI AGTACT 1 cut(s) 382
Bme1390I CCNGG 6 cut(s) 468, 742, 762, 1059, 1068, 1516
Bme18I GGWCC 1 cut(s) 1274
BmgT120I GGNCC 2 cut(s) 971, 1274
BmrFI CCNGG 6 cut(s) 468, 742, 762, 1059, 1068, 1516
BmrI ACTGGG 1 cut(s) 1215
BmsI GCATC 2 cut(s) 787, 1458
BmuI ACTGGG 1 cut(s) 1215
BpiI GAAGAC 2 cut(s) 488, 897
BpmI CTGGAG 1 cut(s) 1160
Bpu10I CCTNAGC 1 cut(s) 824
BpuMI CCSGG 3 cut(s) 742, 762, 1516
BsaJI CCNNGG 6 cut(s) 466, 467, 760, 1350, 1515, 1552
BsaWI WCCGGW 1 cut(s) 152
Bsc4I CCNNNNNNNGG 1 cut(s) 956
Bse1I ACTGG 3 cut(s) 842, 1143, 1210
Bse3DI GCAATG 4 cut(s) 31, 61, 615, 871
BseAI TCCGGA 1 cut(s) 152
BseBI CCWGG 3 cut(s) 468, 1059, 1068
BseDI CCNNGG 6 cut(s) 466, 467, 760, 1350, 1515, 1552
BseGI GGATG 1 cut(s) 1063
BseLI CCNNNNNNNGG 1 cut(s) 956
BseMI GCAATG 4 cut(s) 31, 61, 615, 871
BseMII CTCAG 4 cut(s) 305, 838, 966, 1110
BseNI ACTGG 3 cut(s) 842, 1143, 1210
BseSI GKGCMC 1 cut(s) 541
BseXI GCAGC 2 cut(s) 866, 1249
BsgI GTGCAG 2 cut(s) 91, 670
Bsh1236I CGCG 3 cut(s) 209, 986, 1281
BshFI GGCC 4 cut(s) 857, 973, 1407, 1551
BsiHKAI GWGCWC 2 cut(s) 245, 541
BsiSI CCGG 4 cut(s) 153, 741, 762, 1515
BslI CCNNNNNNNGG 1 cut(s) 956
BsmAI GTCTC 2 cut(s) 1115, 1136
BsmBI CGTCTC 1 cut(s) 1115
BsmI GAATGC 1 cut(s) 807
BsnI GGCC 4 cut(s) 857, 973, 1407, 1551
Bsp1286I GDGCHC 2 cut(s) 245, 541
Bsp13I TCCGGA 1 cut(s) 152
Bsp143I GATC 4 cut(s) 160, 815, 1494, 1602
BspACI CCGC 3 cut(s) 296, 986, 1281
BspANI GGCC 4 cut(s) 857, 973, 1407, 1551
BspCNI CTCAG 4 cut(s) 306, 837, 967, 1111
BspEI TCCGGA 1 cut(s) 152
BspFNI CGCG 3 cut(s) 209, 986, 1281
BspMI ACCTGC 1 cut(s) 642
BsrDI GCAATG 4 cut(s) 31, 61, 615, 871
BsrI ACTGG 3 cut(s) 842, 1143, 1210
BssECI CCNNGG 6 cut(s) 466, 467, 760, 1350, 1515, 1552
BssMI GATC 4 cut(s) 160, 815, 1494, 1602
BssSI CACGAG 1 cut(s) 1326
BssT1I CCWWGG 2 cut(s) 1350, 1552
Bst2BI CACGAG 1 cut(s) 1326
Bst2UI CCWGG 3 cut(s) 468, 1059, 1068
Bst4CI ACNGT 8 cut(s) 186, 423, 477, 664, 850, 1126, 1364, 1435
Bst6I CTCTTC 1 cut(s) 1096
BstDEI CTNAG 4 cut(s) 314, 824, 975, 1119
BstF5I GGATG 1 cut(s) 1063
BstFNI CGCG 3 cut(s) 209, 986, 1281
BstKTI GATC 4 cut(s) 163, 818, 1497, 1605
BstMAI GTCTC 2 cut(s) 1115, 1136
BstMBI GATC 4 cut(s) 160, 815, 1494, 1602
BstMWI GCNNNNNNNGC 4 cut(s) 302, 885, 992, 1009
BstNI CCWGG 3 cut(s) 468, 1059, 1068
BstSCI CCNGG 6 cut(s) 466, 740, 760, 1057, 1066, 1514
BstSFI CTRYAG 2 cut(s) 144, 390
BstSLI GKGCMC 1 cut(s) 541
BstUI CGCG 3 cut(s) 209, 986, 1281
BstV1I GCAGC 2 cut(s) 866, 1249
BstV2I GAAGAC 2 cut(s) 488, 897
BstX2I RGATCY 3 cut(s) 160, 815, 1602
BstYI RGATCY 3 cut(s) 160, 815, 1602
BsuRI GGCC 4 cut(s) 857, 973, 1407, 1551
BtsCI GGATG 1 cut(s) 1063
BtsI GCAGTG 1 cut(s) 79
BtsIMutI CAGTG 2 cut(s) 79, 604
BveI ACCTGC 1 cut(s) 642
CaiI CAGNNNCTG 2 cut(s) 675, 848
Cfr13I GGNCC 2 cut(s) 971, 1274
CseI GACGC 1 cut(s) 215
CsiI ACCWGGT 1 cut(s) 1057
Csp6I GTAC 7 cut(s) 96, 318, 381, 461, 1055, 1087, 1184
CviAII CATG 6 cut(s) 451, 519, 542, 931, 968, 1375
CviQI GTAC 7 cut(s) 96, 318, 381, 461, 1055, 1087, 1184
DdeI CTNAG 4 cut(s) 314, 824, 975, 1119
DpnI GATC 4 cut(s) 162, 817, 1496, 1604
DpnII GATC 4 cut(s) 160, 815, 1494, 1602
DraIII CACNNNGTG 1 cut(s) 1283
EaeI YGGCCR 1 cut(s) 1405
Eam1104I CTCTTC 1 cut(s) 1096
EarI CTCTTC 1 cut(s) 1096
Eco130I CCWWGG 2 cut(s) 1350, 1552
Eco47I GGWCC 1 cut(s) 1274
Eco57I CTGAAG 6 cut(s) 273, 348, 688, 712, 1249, 1403
EcoRI GAATTC 1 cut(s) 1334
EcoRII CCWGG 3 cut(s) 466, 1057, 1066
EcoT14I CCWWGG 2 cut(s) 1350, 1552
ErhI CCWWGG 2 cut(s) 1350, 1552
Esp3I CGTCTC 1 cut(s) 1115
FaeI CATG 6 cut(s) 454, 522, 545, 934, 971, 1378
FatI CATG 6 cut(s) 450, 518, 541, 930, 967, 1374
FauNDI CATATG 1 cut(s) 1582
Fnu4HI GCNGC 3 cut(s) 880, 987, 1238
FokI GGATG 1 cut(s) 1050
Fsp4HI GCNGC 3 cut(s) 880, 987, 1238
FspBI CTAG 3 cut(s) 306, 992, 1606
GluI GCNGC 3 cut(s) 880, 987, 1238
GsuI CTGGAG 1 cut(s) 1160
HaeIII GGCC 4 cut(s) 857, 973, 1407, 1551
HapII CCGG 4 cut(s) 153, 741, 762, 1515
HgaI GACGC 1 cut(s) 215
Hin1II CATG 6 cut(s) 454, 522, 545, 934, 971, 1378
HindIII AAGCTT 1 cut(s) 944
HpaII CCGG 4 cut(s) 153, 741, 762, 1515
HphI GGTGA 3 cut(s) 248, 1408, 1504
Hpy166II GTNNAC 6 cut(s) 278, 539, 928, 1184, 1429, 1588
Hpy188III TCNNGA 1 cut(s) 153
Hpy8I GTNNAC 6 cut(s) 278, 539, 928, 1184, 1429, 1588
Hpy99I CGWCG 2 cut(s) 537, 1406
HpyAV CCTTC 6 cut(s) 283, 1014, 1057, 1151, 1225, 1273
HpyCH4III ACNGT 8 cut(s) 186, 423, 477, 664, 850, 1126, 1364, 1435
HpyCH4V TGCA 8 cut(s) 72, 522, 539, 608, 651, 864, 918, 1600
HpyF10VI GCNNNNNNNGC 4 cut(s) 302, 885, 992, 1009
HpyF3I CTNAG 4 cut(s) 314, 824, 975, 1119
Hsp92II CATG 6 cut(s) 454, 522, 545, 934, 971, 1378
Kpn2I TCCGGA 1 cut(s) 152
Kzo9I GATC 4 cut(s) 160, 815, 1494, 1602
LmnI GCTCC 1 cut(s) 1234
Lsp1109I GCAGC 2 cut(s) 866, 1249
LweI GCATC 2 cut(s) 787, 1458
MabI ACCWGGT 1 cut(s) 1057
MaeI CTAG 3 cut(s) 306, 992, 1606
MaeIII GTNAC 4 cut(s) 563, 707, 844, 1313
MalI GATC 4 cut(s) 162, 817, 1496, 1604
MboI GATC 4 cut(s) 160, 815, 1494, 1602
MflI RGATCY 3 cut(s) 160, 815, 1602
MhlI GDGCHC 2 cut(s) 245, 541
MluCI AATT 6 cut(s) 61, 109, 490, 1334, 1392, 1473
MluI ACGCGT 1 cut(s) 207
MlyI GAGTC 6 cut(s) 23, 133, 218, 224, 666, 986
MmeI TCCRAC 3 cut(s) 151, 1134, 1462
MroI TCCGGA 1 cut(s) 152
MroXI GAANNNNTTC 2 cut(s) 258, 333
MseI TTAA 1 cut(s) 1202
MslI CAYNNNNRTG 2 cut(s) 517, 1068
MspI CCGG 4 cut(s) 153, 741, 762, 1515
MspR9I CCNGG 6 cut(s) 468, 742, 762, 1059, 1068, 1516
Mva1269I GAATGC 1 cut(s) 807
MvaI CCWGG 3 cut(s) 468, 1059, 1068
MvnI CGCG 3 cut(s) 209, 986, 1281
MwoI GCNNNNNNNGC 4 cut(s) 302, 885, 992, 1009
NciI CCSGG 3 cut(s) 742, 762, 1516
NdeI CATATG 1 cut(s) 1582
NdeII GATC 4 cut(s) 160, 815, 1494, 1602
NlaIII CATG 6 cut(s) 454, 522, 545, 934, 971, 1378
NmuCI GTSAC 2 cut(s) 563, 707
PasI CCCWGGG 1 cut(s) 467
PctI GAATGC 1 cut(s) 807
PdmI GAANNNNTTC 2 cut(s) 258, 333
PfeI GAWTC 8 cut(s) 35, 77, 101, 149, 323, 386, 411, 528
PkrI GCNGC 3 cut(s) 881, 988, 1239
PleI GAGTC 6 cut(s) 23, 132, 218, 224, 666, 985
PpsI GAGTC 6 cut(s) 23, 132, 218, 224, 666, 985
PsiI TTATAA 1 cut(s) 579
Psp6I CCWGG 3 cut(s) 466, 1057, 1066
PspGI CCWGG 3 cut(s) 466, 1057, 1066
PspPI GGNCC 2 cut(s) 971, 1274
PstNI CAGNNNCTG 2 cut(s) 675, 848
PsuI RGATCY 3 cut(s) 160, 815, 1602
RsaI GTAC 7 cut(s) 97, 319, 382, 462, 1056, 1088, 1185
RsaNI GTAC 7 cut(s) 96, 318, 381, 461, 1055, 1087, 1184
RseI CAYNNNNRTG 2 cut(s) 517, 1068
SaqAI TTAA 1 cut(s) 1202
SatI GCNGC 3 cut(s) 880, 987, 1238
Sau3AI GATC 4 cut(s) 160, 815, 1494, 1602
Sau96I GGNCC 2 cut(s) 971, 1274
ScaI AGTACT 1 cut(s) 382
SchI GAGTC 6 cut(s) 23, 133, 218, 224, 666, 986
ScrFI CCNGG 6 cut(s) 468, 742, 762, 1059, 1068, 1516
SduI GDGCHC 2 cut(s) 245, 541
SexAI ACCWGGT 1 cut(s) 1057
SfaNI GCATC 2 cut(s) 787, 1458
SfcI CTRYAG 2 cut(s) 144, 390
SinI GGWCC 1 cut(s) 1274
SmiMI CAYNNNNRTG 2 cut(s) 517, 1068
Sse9I AATT 6 cut(s) 61, 109, 490, 1334, 1392, 1473
SsiI CCGC 3 cut(s) 296, 986, 1281
SspMI CTAG 3 cut(s) 306, 992, 1606
StyD4I CCNGG 6 cut(s) 466, 740, 760, 1057, 1066, 1514
StyI CCWWGG 2 cut(s) 1350, 1552
TaaI ACNGT 8 cut(s) 186, 423, 477, 664, 850, 1126, 1364, 1435
TaqI TCGA 2 cut(s) 188, 1224
TaqII GACCGA 1 cut(s) 1328
TasI AATT 6 cut(s) 61, 109, 490, 1334, 1392, 1473
TatI WGTACW 4 cut(s) 380, 460, 1086, 1183
TauI GCSGC 1 cut(s) 989
TfiI GAWTC 8 cut(s) 35, 77, 101, 149, 323, 386, 411, 528
Tru1I TTAA 1 cut(s) 1202
Tru9I TTAA 1 cut(s) 1202
TscAI CASTG 2 cut(s) 79, 604
TseFI GTSAC 2 cut(s) 563, 707
TseI GCWGC 2 cut(s) 879, 1237
Tsp45I GTSAC 2 cut(s) 563, 707
TspDTI ATGAA 4 cut(s) 285, 1149, 1313, 1581
TspGWI ACGGA 2 cut(s) 521, 1163
TspRI CASTG 2 cut(s) 79, 604
VneI GTGCAC 1 cut(s) 537
VpaK11BI GGWCC 1 cut(s) 1274
XapI RAATTY 2 cut(s) 1334, 1392
XmnI GAANNNNTTC 2 cut(s) 258, 333
XspI CTAG 3 cut(s) 306, 992, 1606
ZrmI AGTACT 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.