MD02G1218000.v1.1

HVA22-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0004929
Physical Location & Seq
Forward (+)
25327607 .. 25328084
478 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1218000.v1.1.491

Sequence Viewer

Length: 408 bp
ATGATAGGATCCTTTCTCACCAGAACACTTGTGATGACTCTTGGGTATGCTTATCCGGCTTATGAATGCTACAAAACTGTTGAAAAGAACAAGCCAGAGATTGAACAGCTTCGCTTTTGGTGCCAGTATTGGATTTTGGTGGCTGTTTTGACTGTTTGTGAAAGAGTCGGTTATGCTTTTGTTTCATGGGTTCCAATGTATAGCGAAGCTAAGTTGCTCTTCGTCATATATCTGTGGTTTCCTAAAACAAAGGGTACGAGCTATGTGTATGATTCCTTCTTTAGACCATATCTTGCGAAGCATGAAAATGAAATCGATAGGAACTTGTTGGAACTAAGAACTAGAGCTGTATGTTGCCGCACAATCGACACCAAGGCCTCGCCCTACGCAGCCACAACAAGGTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TB2_DP1_HVA22

Protein Analysis

136

Amino Acids

15.94

Weight (kDa)

8.88

Isoelectric Point (pI)

41.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TB2_DP1_HVA22 PF03134 19 - 97 1.2e-25 TB2/DP1, HVA22 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 120
AciI CCGC 1 cut(s) 358
AclWI GGATC 2 cut(s) 3, 16
AfaI GTAC 1 cut(s) 256
AfiI CCNNNNNNNGG 1 cut(s) 399
AgsI TTSAA 2 cut(s) 83, 104
AluBI AGCT 4 cut(s) 109, 209, 261, 347
AluI AGCT 4 cut(s) 109, 209, 261, 347
AlwI GGATC 2 cut(s) 3, 16
AoxI GGCC 1 cut(s) 375
ApeKI GCWGC 1 cut(s) 389
Asp700I GAANNNNTTC 1 cut(s) 108
AsuHPI GGTGA 1 cut(s) 10
BamHI GGATCC 1 cut(s) 8
BanI GGYRCC 1 cut(s) 120
BarI GAAGNNNNNNTAC 2 cut(s) 260, 292
BbvI GCAGC 1 cut(s) 401
BfaI CTAG 1 cut(s) 342
BisI GCNGC 2 cut(s) 358, 390
BlsI GCNGC 2 cut(s) 359, 391
BmiI GGNNCC 3 cut(s) 10, 122, 192
Bsa29I ATCGAT 1 cut(s) 315
BsaJI CCNNGG 1 cut(s) 372
Bsc4I CCNNNNNNNGG 1 cut(s) 399
Bse1I ACTGG 1 cut(s) 124
BseCI ATCGAT 1 cut(s) 315
BseDI CCNNGG 1 cut(s) 372
BseLI CCNNNNNNNGG 1 cut(s) 399
BseNI ACTGG 1 cut(s) 124
BseXI GCAGC 1 cut(s) 401
BshFI GGCC 1 cut(s) 377
BshNI GGYRCC 1 cut(s) 120
BshVI ATCGAT 1 cut(s) 315
BsiSI CCGG 1 cut(s) 56
BslI CCNNNNNNNGG 1 cut(s) 399
BsmI GAATGC 1 cut(s) 71
BsnI GGCC 1 cut(s) 377
Bsp143I GATC 1 cut(s) 8
BspACI CCGC 1 cut(s) 358
BspANI GGCC 1 cut(s) 377
BspDI ATCGAT 1 cut(s) 315
BspLI GGNNCC 3 cut(s) 10, 122, 192
BspPI GGATC 2 cut(s) 3, 16
BspQI GCTCTTC 1 cut(s) 224
BspT107I GGYRCC 1 cut(s) 120
BsrI ACTGG 1 cut(s) 124
BssECI CCNNGG 1 cut(s) 372
BssMI GATC 1 cut(s) 8
BssT1I CCWWGG 1 cut(s) 372
Bst4CI ACNGT 2 cut(s) 79, 154
Bst6I CTCTTC 1 cut(s) 224
BstDEI CTNAG 2 cut(s) 210, 335
BstKTI GATC 1 cut(s) 11
BstMBI GATC 1 cut(s) 8
BstMWI GCNNNNNNNGC 2 cut(s) 56, 120
BstV1I GCAGC 1 cut(s) 401
BstX2I RGATCY 1 cut(s) 8
BstYI RGATCY 1 cut(s) 8
Bsu15I ATCGAT 1 cut(s) 315
BsuRI GGCC 1 cut(s) 377
BsuTUI ATCGAT 1 cut(s) 315
ClaI ATCGAT 1 cut(s) 315
Csp6I GTAC 1 cut(s) 255
CviAII CATG 2 cut(s) 186, 302
CviJI RGCY 9 cut(s) 59, 94, 109, 143, 209, 261, 347, 377, 392
CviKI_1 RGCY 9 cut(s) 59, 94, 109, 143, 209, 261, 347, 377, 392
CviQI GTAC 1 cut(s) 255
DdeI CTNAG 2 cut(s) 210, 335
DpnI GATC 1 cut(s) 10
DpnII GATC 1 cut(s) 8
Eam1104I CTCTTC 1 cut(s) 224
EarI CTCTTC 1 cut(s) 224
Eco130I CCWWGG 1 cut(s) 372
Eco147I AGGCCT 1 cut(s) 377
EcoT14I CCWWGG 1 cut(s) 372
ErhI CCWWGG 1 cut(s) 372
FaeI CATG 2 cut(s) 189, 305
FalI AAGNNNNNCTT 2 cut(s) 203, 235
FatI CATG 2 cut(s) 185, 301
Fnu4HI GCNGC 2 cut(s) 358, 390
Fsp4HI GCNGC 2 cut(s) 358, 390
FspBI CTAG 1 cut(s) 342
GluI GCNGC 2 cut(s) 358, 390
HaeIII GGCC 1 cut(s) 377
HapII CCGG 1 cut(s) 56
Hin1II CATG 2 cut(s) 189, 305
HinfI GANTC 3 cut(s) 37, 165, 272
HpaII CCGG 1 cut(s) 56
HphI GGTGA 1 cut(s) 10
HpyAV CCTTC 1 cut(s) 286
HpyCH4III ACNGT 2 cut(s) 79, 154
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 120
HpyF3I CTNAG 2 cut(s) 210, 335
Hsp92II CATG 2 cut(s) 189, 305
Kzo9I GATC 1 cut(s) 8
LguI GCTCTTC 1 cut(s) 224
LpnPI CCDG 4 cut(s) 34, 69, 108, 137
Lsp1109I GCAGC 1 cut(s) 401
MaeI CTAG 1 cut(s) 342
MalI GATC 1 cut(s) 10
MboI GATC 1 cut(s) 8
MboII GAAGA 1 cut(s) 211
MflI RGATCY 1 cut(s) 8
MlyI GAGTC 2 cut(s) 31, 174
MmeI TCCRAC 1 cut(s) 309
MnlI CCTC 1 cut(s) 388
MroXI GAANNNNTTC 1 cut(s) 108
MslI CAYNNNNRTG 1 cut(s) 306
MspI CCGG 1 cut(s) 56
Mva1269I GAATGC 1 cut(s) 71
MwoI GCNNNNNNNGC 2 cut(s) 56, 120
NdeII GATC 1 cut(s) 8
NlaIII CATG 2 cut(s) 189, 305
NlaIV GGNNCC 3 cut(s) 10, 122, 192
PceI AGGCCT 1 cut(s) 377
PciSI GCTCTTC 1 cut(s) 224
PctI GAATGC 1 cut(s) 71
PdmI GAANNNNTTC 1 cut(s) 108
PfeI GAWTC 1 cut(s) 272
PkrI GCNGC 2 cut(s) 359, 391
PleI GAGTC 2 cut(s) 31, 173
PpsI GAGTC 2 cut(s) 31, 173
PspN4I GGNNCC 3 cut(s) 10, 122, 192
PsuI RGATCY 1 cut(s) 8
RsaI GTAC 1 cut(s) 256
RsaNI GTAC 1 cut(s) 255
RseI CAYNNNNRTG 1 cut(s) 306
SapI GCTCTTC 1 cut(s) 224
SatI GCNGC 2 cut(s) 358, 390
Sau3AI GATC 1 cut(s) 8
SchI GAGTC 2 cut(s) 31, 174
SetI ASST 5 cut(s) 111, 211, 263, 349, 404
SmiMI CAYNNNNRTG 1 cut(s) 306
SseBI AGGCCT 1 cut(s) 377
SsiI CCGC 1 cut(s) 358
SspMI CTAG 1 cut(s) 342
StuI AGGCCT 1 cut(s) 377
StyI CCWWGG 1 cut(s) 372
TaaI ACNGT 2 cut(s) 79, 154
TaqI TCGA 2 cut(s) 315, 366
TauI GCSGC 1 cut(s) 360
TfiI GAWTC 1 cut(s) 272
TseI GCWGC 1 cut(s) 389
TspDTI ATGAA 4 cut(s) 78, 174, 318, 324
XmnI GAANNNNTTC 1 cut(s) 108
XspI CTAG 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.