MD15G1013400.v1.1

HVA22-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004929
Physical Location & Seq
Forward (+)
737089 .. 740323
3235 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1013400.v1.1.491

Sequence Viewer

Length: 888 bp
ATGATAGGATCCTTTCTTACCAGAACACTTGTGATGGCTCTTGGGTATGCTTATCCAGCTTATGAATGCTACAAAACTGTTGAAAAGAACAAGCCAGAGATTGAACAGCTTCGCTTTTGGTGCCAGTATTGGATTTTGGTGGCTGTTTTGACTGTTTGTGAGAGAGTTGGTGATGCTTTTGTTTCATGGGTTCCAATGTATAGTGAAGCTAAGTTGCTCTTCATTATATATCTGTGGTTTCCTAAAACAAAGGGTACGAGCTATGTGTATGATTCCTTCTTTAGACCATATCTTGCGAAGCATGAAAATGAAATTGATAGGAACTTGTTGGAACTAAGAACTAGAGCTGGTGACATGGCAATTTTGTATTGGCAAAGAGCTGCAAGCTATAGTCAGACGAGAATATTTGACATTTTGCAGTATGTTGCTGCACAATCGACACCAAGGCCTCGCCCTGCGCAGCCACAACAAGGTGTTAGGCGCCAACCGCCTGCTGCTCGCCAACCCCCTGCCGATCCAGCTCCAAATCGCCAACCAGCTGCAACAACGCAAGCACAACCCGAAGAACCACCATCTCCCACTTCCAGTACTTCTTCAAGCAAGGACCAAAAGGAGATAGCCGAGGCCTTAGCACCTTCACCAAACCCTAAACCTGCCCTGCAAGCAGCAGGTTCAGTTACTTTAAAAGCAACAGCAACAGCAGCTGCATCTGAATCATCGAGCCAAGCTACCCCAACTGAAGAGGTTGCACCAATGCAGATTGACGCAGTGCCATCCTCTTCAGCGACTGAAGAGACAAATCCTCCCCCGAAAGAGACAGTTATGGAGGAAACCATTCGGCGCACACGTGGAAGGTTGAGGAAAGCTCGTTCTGCAGGAAGCCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TB2_DP1_HVA22

Protein Analysis

296

Amino Acids

32.83

Weight (kDa)

9.15

Isoelectric Point (pI)

68.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TB2_DP1_HVA22 PF03134 19 - 97 4.4e-26 TB2/DP1, HVA22 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 459
Acc36I ACCTGC 2 cut(s) 659, 661
AccB1I GGYRCC 2 cut(s) 120, 480
AciI CCGC 1 cut(s) 488
AclWI GGATC 3 cut(s) 3, 16, 509
AcuI CTGAAG 3 cut(s) 759, 765, 810
AcvI CACGTG 1 cut(s) 848
AcyI GRCGYC 1 cut(s) 481
AfaI GTAC 2 cut(s) 256, 589
AfiI CCNNNNNNNGG 1 cut(s) 470
AflIII ACRYGT 1 cut(s) 845
AgsI TTSAA 3 cut(s) 83, 104, 597
AloI GAACNNNNNNTCC 2 cut(s) 853, 885
Alw26I GTCTC 2 cut(s) 788, 809
AlwI GGATC 3 cut(s) 3, 16, 509
AlwNI CAGNNNCTG 2 cut(s) 704, 788
AoxI GGCC 2 cut(s) 446, 624
ApeKI GCWGC 8 cut(s) 380, 428, 460, 494, 539, 665, 701, 704
Asp700I GAANNNNTTC 2 cut(s) 108, 834
AspLEI GCGC 3 cut(s) 460, 483, 843
AspS9I GGNCC 1 cut(s) 604
AsuHPI GGTGA 3 cut(s) 182, 362, 630
AvaII GGWCC 1 cut(s) 604
BamHI GGATCC 1 cut(s) 8
BanI GGYRCC 2 cut(s) 120, 480
BarI GAAGNNNNNNTAC 2 cut(s) 260, 292
BbrPI CACGTG 1 cut(s) 848
BbvI GCAGC 8 cut(s) 367, 415, 472, 481, 526, 677, 691, 713
BccI CCATC 3 cut(s) 28, 580, 781
BceAI ACGGC 1 cut(s) 867
BcoDI GTCTC 2 cut(s) 788, 809
BfaI CTAG 1 cut(s) 342
BfmI CTRYAG 2 cut(s) 388, 873
BfoI RGCGCY 1 cut(s) 484
BfuAI ACCTGC 2 cut(s) 659, 661
BisI GCNGC 8 cut(s) 381, 429, 461, 495, 540, 666, 702, 705
BlsI GCNGC 8 cut(s) 382, 430, 462, 496, 541, 667, 703, 706
BmcAI AGTACT 1 cut(s) 589
Bme18I GGWCC 1 cut(s) 604
BmgT120I GGNCC 1 cut(s) 604
BmiI GGNNCC 4 cut(s) 10, 122, 192, 482
BmsI GCATC 2 cut(s) 163, 716
BplI GAGNNNNNCTC 2 cut(s) 850, 882
Bpu10I CCTNAGC 1 cut(s) 628
BsaAI YACGTR 1 cut(s) 848
BsaHI GRCGYC 1 cut(s) 481
BsaJI CCNNGG 2 cut(s) 443, 621
BsaXI ACNNNNNCTCC 2 cut(s) 787, 817
Bsc4I CCNNNNNNNGG 1 cut(s) 470
Bse1I ACTGG 2 cut(s) 124, 585
BseDI CCNNGG 2 cut(s) 443, 621
BseGI GGATG 1 cut(s) 773
BseLI CCNNNNNNNGG 1 cut(s) 470
BseNI ACTGG 2 cut(s) 124, 585
BseXI GCAGC 8 cut(s) 367, 415, 472, 481, 526, 677, 691, 713
BsgI GTGCAG 1 cut(s) 414
BshFI GGCC 2 cut(s) 448, 626
BshNI GGYRCC 2 cut(s) 120, 480
BslI CCNNNNNNNGG 1 cut(s) 470
BsmAI GTCTC 2 cut(s) 788, 809
BsmI GAATGC 1 cut(s) 71
BsnI GGCC 2 cut(s) 448, 626
Bsp143I GATC 2 cut(s) 8, 514
BspACI CCGC 1 cut(s) 488
BspANI GGCC 2 cut(s) 448, 626
BspLI GGNNCC 4 cut(s) 10, 122, 192, 482
BspMAI CTGCAG 1 cut(s) 877
BspMI ACCTGC 2 cut(s) 659, 661
BspPI GGATC 3 cut(s) 3, 16, 509
BspQI GCTCTTC 1 cut(s) 224
BspT107I GGYRCC 2 cut(s) 120, 480
BsrI ACTGG 2 cut(s) 124, 585
BssECI CCNNGG 2 cut(s) 443, 621
BssMI GATC 2 cut(s) 8, 514
BssNI GRCGYC 1 cut(s) 481
BssT1I CCWWGG 1 cut(s) 443
Bst4CI ACNGT 3 cut(s) 79, 154, 820
Bst6I CTCTTC 4 cut(s) 224, 735, 784, 786
BstACI GRCGYC 1 cut(s) 481
BstBAI YACGTR 1 cut(s) 848
BstC8I GCNNGC 5 cut(s) 385, 492, 499, 552, 663
BstDEI CTNAG 3 cut(s) 210, 335, 628
BstF5I GGATG 1 cut(s) 773
BstH2I RGCGCY 1 cut(s) 484
BstHHI GCGC 3 cut(s) 460, 483, 843
BstKTI GATC 2 cut(s) 11, 517
BstMAI GTCTC 2 cut(s) 788, 809
BstMBI GATC 2 cut(s) 8, 514
BstMWI GCNNNNNNNGC 7 cut(s) 56, 120, 487, 518, 662, 701, 872
BstSFI CTRYAG 2 cut(s) 388, 873
BstV1I GCAGC 8 cut(s) 367, 415, 472, 481, 526, 677, 691, 713
BstX2I RGATCY 1 cut(s) 8
BstYI RGATCY 1 cut(s) 8
BsuRI GGCC 2 cut(s) 448, 626
BtsCI GGATG 1 cut(s) 773
BtsI GCAGTG 1 cut(s) 774
BtsIMutI CAGTG 1 cut(s) 774
BveI ACCTGC 2 cut(s) 659, 661
Cac8I GCNNGC 5 cut(s) 385, 492, 499, 552, 663
CaiI CAGNNNCTG 2 cut(s) 704, 788
CfoI GCGC 3 cut(s) 460, 483, 843
Cfr13I GGNCC 1 cut(s) 604
CseI GACGC 1 cut(s) 773
Csp6I GTAC 2 cut(s) 255, 588
CviAII CATG 3 cut(s) 186, 302, 355
CviQI GTAC 2 cut(s) 255, 588
DdeI CTNAG 3 cut(s) 210, 335, 628
DinI GGCGCC 1 cut(s) 482
DpnI GATC 2 cut(s) 10, 516
DpnII GATC 2 cut(s) 8, 514
DraI TTTAAA 1 cut(s) 684
Eam1104I CTCTTC 4 cut(s) 224, 735, 784, 786
EarI CTCTTC 4 cut(s) 224, 735, 784, 786
Eco130I CCWWGG 1 cut(s) 443
Eco147I AGGCCT 2 cut(s) 448, 626
Eco47I GGWCC 1 cut(s) 604
Eco57I CTGAAG 3 cut(s) 759, 765, 810
Eco72I CACGTG 1 cut(s) 848
EcoT14I CCWWGG 1 cut(s) 443
EgeI GGCGCC 1 cut(s) 482
EheI GGCGCC 1 cut(s) 482
ErhI CCWWGG 1 cut(s) 443
FaeI CATG 3 cut(s) 189, 305, 358
FalI AAGNNNNNCTT 2 cut(s) 203, 235
FatI CATG 3 cut(s) 185, 301, 354
Fnu4HI GCNGC 8 cut(s) 381, 429, 461, 495, 540, 666, 702, 705
FokI GGATG 1 cut(s) 760
Fsp4HI GCNGC 8 cut(s) 381, 429, 461, 495, 540, 666, 702, 705
FspBI CTAG 1 cut(s) 342
FspI TGCGCA 1 cut(s) 459
GlaI GCGC 3 cut(s) 459, 482, 842
GluI GCNGC 8 cut(s) 381, 429, 461, 495, 540, 666, 702, 705
HaeII RGCGCY 1 cut(s) 484
HaeIII GGCC 2 cut(s) 448, 626
HgaI GACGC 1 cut(s) 773
HhaI GCGC 3 cut(s) 460, 483, 843
Hin1I GRCGYC 1 cut(s) 481
Hin1II CATG 3 cut(s) 189, 305, 358
Hin6I GCGC 3 cut(s) 458, 481, 841
HinP1I GCGC 3 cut(s) 458, 481, 841
HinfI GANTC 2 cut(s) 272, 713
HphI GGTGA 3 cut(s) 182, 362, 630
Hpy188I TCNGA 2 cut(s) 396, 712
HpyAV CCTTC 3 cut(s) 286, 645, 846
HpyCH4III ACNGT 3 cut(s) 79, 154, 820
HpyCH4IV ACGT 1 cut(s) 847
HpyCH4V TGCA 9 cut(s) 383, 418, 431, 542, 661, 707, 749, 757, 875
HpyF10VI GCNNNNNNNGC 7 cut(s) 56, 120, 487, 518, 662, 701, 872
HpyF3I CTNAG 3 cut(s) 210, 335, 628
HpySE526I ACGT 1 cut(s) 847
Hsp92I GRCGYC 1 cut(s) 481
Hsp92II CATG 3 cut(s) 189, 305, 358
HspAI GCGC 3 cut(s) 458, 481, 841
KasI GGCGCC 1 cut(s) 480
Kzo9I GATC 2 cut(s) 8, 514
LguI GCTCTTC 1 cut(s) 224
LmnI GCTCC 1 cut(s) 526
Lsp1109I GCAGC 8 cut(s) 367, 415, 472, 481, 526, 677, 691, 713
LweI GCATC 2 cut(s) 163, 716
MaeI CTAG 1 cut(s) 342
MaeII ACGT 1 cut(s) 847
MaeIII GTNAC 2 cut(s) 350, 676
MalI GATC 2 cut(s) 10, 516
MboI GATC 2 cut(s) 8, 514
MboII GAAGA 6 cut(s) 211, 575, 585, 752, 771, 803
MflI RGATCY 1 cut(s) 8
MluCI AATT 2 cut(s) 312, 360
Mly113I GGCGCC 1 cut(s) 481
MmeI TCCRAC 1 cut(s) 309
MnlI CCTC 7 cut(s) 459, 616, 736, 787, 813, 820, 852
MroXI GAANNNNTTC 2 cut(s) 108, 834
MseI TTAA 1 cut(s) 683
MslI CAYNNNNRTG 1 cut(s) 306
MspA1I CMGCKG 2 cut(s) 539, 704
Mva1269I GAATGC 1 cut(s) 71
MwoI GCNNNNNNNGC 7 cut(s) 56, 120, 487, 518, 662, 701, 872
NarI GGCGCC 1 cut(s) 481
NdeII GATC 2 cut(s) 8, 514
NlaIII CATG 3 cut(s) 189, 305, 358
NlaIV GGNNCC 4 cut(s) 10, 122, 192, 482
NmeAIII GCCGAG 1 cut(s) 646
NmuCI GTSAC 1 cut(s) 350
NsbI TGCGCA 1 cut(s) 459
PceI AGGCCT 2 cut(s) 448, 626
PciSI GCTCTTC 1 cut(s) 224
PcsI WCGNNNNNNNCGW 1 cut(s) 844
PctI GAATGC 1 cut(s) 71
PdmI GAANNNNTTC 2 cut(s) 108, 834
PfeI GAWTC 2 cut(s) 272, 713
PkrI GCNGC 8 cut(s) 382, 430, 462, 496, 541, 667, 703, 706
PluTI GGCGCC 1 cut(s) 484
PmaCI CACGTG 1 cut(s) 848
PmlI CACGTG 1 cut(s) 848
Ppu21I YACGTR 1 cut(s) 848
PspCI CACGTG 1 cut(s) 848
PspN4I GGNNCC 4 cut(s) 10, 122, 192, 482
PspPI GGNCC 1 cut(s) 604
PstI CTGCAG 1 cut(s) 877
PstNI CAGNNNCTG 2 cut(s) 704, 788
PsuI RGATCY 1 cut(s) 8
PvuII CAGCTG 2 cut(s) 539, 704
RsaI GTAC 2 cut(s) 256, 589
RsaNI GTAC 2 cut(s) 255, 588
RseI CAYNNNNRTG 1 cut(s) 306
SapI GCTCTTC 1 cut(s) 224
SaqAI TTAA 1 cut(s) 683
SatI GCNGC 8 cut(s) 381, 429, 461, 495, 540, 666, 702, 705
Sau3AI GATC 2 cut(s) 8, 514
Sau96I GGNCC 1 cut(s) 604
ScaI AGTACT 1 cut(s) 589
SfaNI GCATC 2 cut(s) 163, 716
SfcI CTRYAG 2 cut(s) 388, 873
SfoI GGCGCC 1 cut(s) 482
SinI GGWCC 1 cut(s) 604
SmiMI CAYNNNNRTG 1 cut(s) 306
Sse9I AATT 2 cut(s) 312, 360
SseBI AGGCCT 2 cut(s) 448, 626
SsiI CCGC 1 cut(s) 488
SspDI GGCGCC 1 cut(s) 480
SspI AATATT 1 cut(s) 405
SspMI CTAG 1 cut(s) 342
StuI AGGCCT 2 cut(s) 448, 626
StyI CCWWGG 1 cut(s) 443
TaaI ACNGT 3 cut(s) 79, 154, 820
TaiI ACGT 1 cut(s) 850
TaqI TCGA 2 cut(s) 437, 719
TasI AATT 2 cut(s) 312, 360
TatI WGTACW 1 cut(s) 587
TfiI GAWTC 2 cut(s) 272, 713
Tru1I TTAA 1 cut(s) 683
Tru9I TTAA 1 cut(s) 683
TscAI CASTG 1 cut(s) 774
TseFI GTSAC 1 cut(s) 350
TseI GCWGC 8 cut(s) 380, 428, 460, 494, 539, 665, 701, 704
Tsp45I GTSAC 1 cut(s) 350
TspDTI ATGAA 5 cut(s) 78, 174, 211, 318, 324
TspRI CASTG 1 cut(s) 774
VpaK11BI GGWCC 1 cut(s) 604
XmnI GAANNNNTTC 2 cut(s) 108, 834
XspI CTAG 1 cut(s) 342
ZrmI AGTACT 1 cut(s) 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.