MD05G1114200.v1.1

HVA22-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004929
Physical Location & Seq
Forward (+)
23293725 .. 23295330
1606 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1114200.v1.1.491

Sequence Viewer

Length: 642 bp
ATGGTGGAATCTTTGCTTCCAAGGGTATTTGTGATGGTGTTTGGTTACGCTTATCCGGCGTATGAGTGCTACAAGACTCTGGAGAACAATAAGCCAGAGATTGAGCAACTTGTATTTTGGTGCCAGTACTGGATACTAGTTGCTATGCTTACTGTATTGGAGAGAATTGGGGATGCCTTATTTGCATGGTTACCAATATACTGTGAAGCCAAATTGGCATTGTTTATATATCTCTGGTCTTCTAAAGCGAAAGGAACGAGTTATCTTTACAATCACTTCTTCCGGAATTTTGTTTCACAACATGAGTTAGAGATTGATAGTAAAATATTAGAGTTAAGGGTTAAGACTGGGGATATTGCAAACTACTGTTGCCAAAAGGCTCTGAGCTATGGCCACAAAGGTGTTTCTGAGATTTTTCAGTATGTGTCTTCCCATTCGGCGTCACAGCCTCGTCGCGACCAGAAGCAAGAGGAGTGTACTGAAAGAGTAATTGCTTCCGGCCAGTCTGAAGCTGAAGCAACAGACCAAAGAACACGAGAATGCAAATTTGAAGATAAACCAACTGAATCAGTGACATCGTCTGGAAATGGAATGCCAATTTCTCCCAACAGAAAACGATGGTTAAGAAAGTTCATGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TB2_DP1_HVA22

Protein Analysis

214

Amino Acids

24.83

Weight (kDa)

8.1

Isoelectric Point (pI)

56.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TB2_DP1_HVA22 PF03134 19 - 97 2.7e-22 TB2/DP1, HVA22 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 120
AccII CGCG 1 cut(s) 456
AccIII TCCGGA 1 cut(s) 282
AcoI YGGCCR 2 cut(s) 391, 499
AcsI RAATTY 2 cut(s) 286, 545
AcuI CTGAAG 2 cut(s) 528, 534
AcyI GRCGYC 1 cut(s) 440
AfaI GTAC 2 cut(s) 128, 478
AgsI TTSAA 1 cut(s) 551
AhlI ACTAGT 1 cut(s) 136
AleI CACNNNNGTG 1 cut(s) 399
AluBI AGCT 2 cut(s) 387, 512
AluI AGCT 2 cut(s) 387, 512
Aor13HI TCCGGA 1 cut(s) 282
AoxI GGCC 2 cut(s) 391, 499
ApoI RAATTY 2 cut(s) 286, 545
BalI TGGCCA 1 cut(s) 393
BanI GGYRCC 1 cut(s) 120
BauI CACGAG 1 cut(s) 534
BbsI GAAGAC 2 cut(s) 231, 420
BccI CCATC 2 cut(s) 28, 612
BciVI GTATCC 1 cut(s) 126
BcuI ACTAGT 1 cut(s) 136
BfaI CTAG 1 cut(s) 137
BfuI GTATCC 1 cut(s) 126
BglI GCCNNNNNGGC 1 cut(s) 215
BmcAI AGTACT 1 cut(s) 128
BmiI GGNNCC 1 cut(s) 122
BmrI ACTGGG 1 cut(s) 357
BmsI GCATC 1 cut(s) 163
BmuI ACTGGG 1 cut(s) 357
BpiI GAAGAC 2 cut(s) 231, 420
BpmI CTGGAG 1 cut(s) 101
BsaHI GRCGYC 1 cut(s) 440
BsaJI CCNNGG 1 cut(s) 20
BsaWI WCCGGW 1 cut(s) 282
Bse1I ACTGG 4 cut(s) 124, 134, 352, 502
BseAI TCCGGA 1 cut(s) 282
BseDI CCNNGG 1 cut(s) 20
BseGI GGATG 1 cut(s) 178
BseMII CTCAG 2 cut(s) 374, 399
BseNI ACTGG 4 cut(s) 124, 134, 352, 502
BseRI GAGGAG 1 cut(s) 485
Bsh1236I CGCG 1 cut(s) 456
BshFI GGCC 2 cut(s) 393, 501
BshNI GGYRCC 1 cut(s) 120
BsiSI CCGG 3 cut(s) 56, 283, 498
BsmI GAATGC 2 cut(s) 545, 597
BsnI GGCC 2 cut(s) 393, 501
Bsp13I TCCGGA 1 cut(s) 282
Bsp68I TCGCGA 1 cut(s) 456
BspANI GGCC 2 cut(s) 393, 501
BspCNI CTCAG 2 cut(s) 375, 400
BspEI TCCGGA 1 cut(s) 282
BspFNI CGCG 1 cut(s) 456
BspHI TCATGA 1 cut(s) 633
BspLI GGNNCC 1 cut(s) 122
BspT107I GGYRCC 1 cut(s) 120
BsrI ACTGG 4 cut(s) 124, 134, 352, 502
BssECI CCNNGG 1 cut(s) 20
BssNI GRCGYC 1 cut(s) 440
BssSI CACGAG 1 cut(s) 534
BssT1I CCWWGG 1 cut(s) 20
Bst2BI CACGAG 1 cut(s) 534
Bst4CI ACNGT 3 cut(s) 154, 203, 368
BstACI GRCGYC 1 cut(s) 440
BstDEI CTNAG 2 cut(s) 383, 408
BstEII GGTNACC 1 cut(s) 189
BstF5I GGATG 1 cut(s) 178
BstFNI CGCG 1 cut(s) 456
BstMWI GCNNNNNNNGC 3 cut(s) 56, 182, 215
BstPI GGTNACC 1 cut(s) 189
BstUI CGCG 1 cut(s) 456
BstV2I GAAGAC 2 cut(s) 231, 420
BsuI GTATCC 1 cut(s) 126
BsuRI GGCC 2 cut(s) 393, 501
BtsCI GGATG 1 cut(s) 178
BtsIMutI CAGTG 1 cut(s) 576
BtuMI TCGCGA 1 cut(s) 456
CciI TCATGA 1 cut(s) 633
CseI GACGC 1 cut(s) 429
Csp6I GTAC 2 cut(s) 127, 477
CviAII CATG 3 cut(s) 186, 302, 634
CviJI RGCY 8 cut(s) 94, 209, 380, 387, 393, 448, 501, 512
CviKI_1 RGCY 8 cut(s) 94, 209, 380, 387, 393, 448, 501, 512
CviQI GTAC 2 cut(s) 127, 477
DdeI CTNAG 2 cut(s) 383, 408
EaeI YGGCCR 2 cut(s) 391, 499
Eco130I CCWWGG 1 cut(s) 20
Eco57I CTGAAG 2 cut(s) 528, 534
Eco91I GGTNACC 1 cut(s) 189
EcoO65I GGTNACC 1 cut(s) 189
EcoT14I CCWWGG 1 cut(s) 20
ErhI CCWWGG 1 cut(s) 20
FaeI CATG 3 cut(s) 189, 305, 637
FatI CATG 3 cut(s) 185, 301, 633
FokI GGATG 1 cut(s) 185
FspBI CTAG 1 cut(s) 137
GsuI CTGGAG 1 cut(s) 101
HaeIII GGCC 2 cut(s) 393, 501
HapII CCGG 3 cut(s) 56, 283, 498
HgaI GACGC 1 cut(s) 429
Hin1I GRCGYC 1 cut(s) 440
Hin1II CATG 3 cut(s) 189, 305, 637
HinfI GANTC 3 cut(s) 8, 76, 566
HpaII CCGG 3 cut(s) 56, 283, 498
Hpy166II GTNNAC 1 cut(s) 477
Hpy188I TCNGA 3 cut(s) 384, 409, 508
Hpy188III TCNNGA 5 cut(s) 80, 283, 455, 582, 634
Hpy8I GTNNAC 1 cut(s) 477
Hpy99I CGWCG 1 cut(s) 456
HpyCH4III ACNGT 3 cut(s) 154, 203, 368
HpyCH4V TGCA 3 cut(s) 185, 359, 543
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 182, 215
HpyF3I CTNAG 2 cut(s) 383, 408
Hsp92I GRCGYC 1 cut(s) 440
Hsp92II CATG 3 cut(s) 189, 305, 637
Kpn2I TCCGGA 1 cut(s) 282
LweI GCATC 1 cut(s) 163
MaeI CTAG 1 cut(s) 137
MaeIII GTNAC 4 cut(s) 44, 189, 441, 571
MboII GAAGA 4 cut(s) 231, 271, 420, 563
MlsI TGGCCA 1 cut(s) 393
MluCI AATT 6 cut(s) 165, 212, 286, 489, 545, 597
MluNI TGGCCA 1 cut(s) 393
MlyI GAGTC 1 cut(s) 70
MnlI CCTC 2 cut(s) 459, 463
Mox20I TGGCCA 1 cut(s) 393
MroI TCCGGA 1 cut(s) 282
MscI TGGCCA 1 cut(s) 393
MseI TTAA 3 cut(s) 335, 342, 623
MslI CAYNNNNRTG 2 cut(s) 399, 538
Msp20I TGGCCA 1 cut(s) 393
MspI CCGG 3 cut(s) 56, 283, 498
Mva1269I GAATGC 2 cut(s) 545, 597
MvnI CGCG 1 cut(s) 456
MwoI GCNNNNNNNGC 3 cut(s) 56, 182, 215
NlaIII CATG 3 cut(s) 189, 305, 637
NlaIV GGNNCC 1 cut(s) 122
NmuCI GTSAC 2 cut(s) 441, 571
NruI TCGCGA 1 cut(s) 456
OliI CACNNNNGTG 1 cut(s) 399
PagI TCATGA 1 cut(s) 633
PctI GAATGC 2 cut(s) 545, 597
PfeI GAWTC 2 cut(s) 8, 566
PflFI GACNNNGTC 1 cut(s) 577
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
PspEI GGTNACC 1 cut(s) 189
PspN4I GGNNCC 1 cut(s) 122
PsyI GACNNNGTC 1 cut(s) 577
RruI TCGCGA 1 cut(s) 456
RsaI GTAC 2 cut(s) 128, 478
RsaNI GTAC 2 cut(s) 127, 477
RseI CAYNNNNRTG 2 cut(s) 399, 538
SaqAI TTAA 3 cut(s) 335, 342, 623
ScaI AGTACT 1 cut(s) 128
SchI GAGTC 1 cut(s) 70
SetI ASST 3 cut(s) 389, 403, 514
SfaNI GCATC 1 cut(s) 163
SmiMI CAYNNNNRTG 2 cut(s) 399, 538
SpeI ACTAGT 1 cut(s) 136
Sse9I AATT 6 cut(s) 165, 212, 286, 489, 545, 597
SspI AATATT 1 cut(s) 327
SspMI CTAG 1 cut(s) 137
StyI CCWWGG 1 cut(s) 20
TaaI ACNGT 3 cut(s) 154, 203, 368
TasI AATT 6 cut(s) 165, 212, 286, 489, 545, 597
TatI WGTACW 2 cut(s) 126, 476
TfiI GAWTC 2 cut(s) 8, 566
Tru1I TTAA 3 cut(s) 335, 342, 623
Tru9I TTAA 3 cut(s) 335, 342, 623
TscAI CASTG 1 cut(s) 576
TseFI GTSAC 2 cut(s) 441, 571
Tsp45I GTSAC 2 cut(s) 441, 571
TspDTI ATGAA 1 cut(s) 622
TspRI CASTG 1 cut(s) 576
Tth111I GACNNNGTC 1 cut(s) 577
XapI RAATTY 2 cut(s) 286, 545
XspI CTAG 1 cut(s) 137
ZrmI AGTACT 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.