Rorug06G0316300

HVA22-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0004929
Physical Location & Seq
Reverse (-)
46769786 .. 46774127
4342 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0316300.1

Sequence Viewer

Length: 891 bp
ATGGCCAACAACCCCTCCGAAGCTCAGGCCGACGATTTCCTCGAGCAAATTCTCGGCCTCCCCAATTTCGCCTCCGCCGATGCTAATTTGACCGGAGGCGACGGAGGCCTCGCCGGAGCTCCCTCCTCGATGATGCTCCAGCTCAGTTCCGGTGACGGCTCCGGCCACATGGGCGGCGTCGGCGGAGGTGGCTACCACGGAGGAGTGTTCCCGTTAGGGTTGAGCCTGGAGCAGGGGAAGGCCGGGTTTTTGAAGCCGGAGGAGGCCTCTGGGAGTGGGAAGCGGTTTCGAGACGACGTCGTTGATAGTAGAGCTTCGTCTGTGAAAAATGTTTTCCATGGGCAACCAATTTCAAGTACAGTTGCTGCAGCACCGCATCCACCGGCTATGCGACCAAGGGTGAGGGCCAGAAGAGGGCAAGCCACGGATCCACATAGCATTGCTGAGCGGTTGCGCCGAGAACGAATAGCCGAAAGAATTAGGGCGTTGCAGGAACTAGTTCCCAGTGTGAACAAGACAGATAGAGCTGCTATGCTTGACGAAATAATGGATTATGTGAAGTTCCTGAGACTCCAAGTAAAGGTTTTGAGTATGAGTAGATTGGGAGGAGCTGGTGCTGTCGCACCACTTGTAACGGACATCCCACTATCTTCAGTTGAGGAAGAAGGTGGTGAAGGTGGTAGAAACCAACCGGCATGGGATAAATGGTCTAATGATGGAACAGAACGACAAGTCGCTAAGCTTATGGAAGAAAATGTTGGAGCTGCAATGCAGTTCCTTCAATCAAAGGCTCTTTGCATCATGCCTATCTCGCTAGCCTCGGCAATTTACCACACTCAACCACCAGACACCTCCAGCGTTGTCAAGTCTGAAATGAATCCCCCTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TB2_DP1_HVA22

Protein Analysis

296

Amino Acids

31.28

Weight (kDa)

5.91

Isoelectric Point (pI)

48.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 145 - 190 1.8e-07 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 300
AccBSI CCGCTC 1 cut(s) 448
AciI CCGC 6 cut(s) 75, 174, 183, 283, 374, 448
AclWI GGATC 2 cut(s) 422, 435
AcoI YGGCCR 2 cut(s) 3, 163
AcsI RAATTY 1 cut(s) 48
AcuI CTGAAG 1 cut(s) 636
AcyI GRCGYC 2 cut(s) 177, 297
AfaI GTAC 1 cut(s) 358
AfiI CCNNNNNNNGG 3 cut(s) 232, 414, 580
AgsI TTSAA 3 cut(s) 253, 354, 782
AhlI ACTAGT 1 cut(s) 496
AjnI CCWGG 1 cut(s) 225
AjuI GAANNNNNNNTTGG 2 cut(s) 741, 773
AluBI AGCT 8 cut(s) 23, 119, 142, 314, 527, 611, 742, 764
AluI AGCT 8 cut(s) 23, 119, 142, 314, 527, 611, 742, 764
Alw21I GWGCWC 1 cut(s) 121
Alw26I GTCTC 2 cut(s) 285, 562
AlwI GGATC 2 cut(s) 422, 435
AlwNI CAGNNNCTG 1 cut(s) 365
Ama87I CYCGRG 1 cut(s) 41
AoxI GGCC 8 cut(s) 3, 27, 55, 106, 163, 240, 264, 405
ApeKI GCWGC 4 cut(s) 365, 368, 527, 764
ApoI RAATTY 1 cut(s) 48
Asp700I GAANNNNTTC 1 cut(s) 498
AspLEI GCGC 1 cut(s) 456
AspS9I GGNCC 1 cut(s) 405
AsuC2I CCSGG 1 cut(s) 244
AsuHPI GGTGA 3 cut(s) 164, 412, 683
AsuNHI GCTAGC 1 cut(s) 814
AvaI CYCGRG 1 cut(s) 41
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 427
BanII GRGCYC 1 cut(s) 121
Bbv12I GWGCWC 1 cut(s) 121
BbvI GCAGC 4 cut(s) 352, 380, 514, 751
BccI CCATC 1 cut(s) 710
BceAI ACGGC 1 cut(s) 172
BciT130I CCWGG 1 cut(s) 227
BcnI CCSGG 1 cut(s) 244
BcoDI GTCTC 2 cut(s) 285, 562
BcuI ACTAGT 1 cut(s) 496
BfaI CTAG 2 cut(s) 497, 815
BfmI CTRYAG 1 cut(s) 366
BglI GCCNNNNNGGC 1 cut(s) 171
BisI GCNGC 5 cut(s) 175, 366, 369, 528, 765
BlpI GCTNAGC 2 cut(s) 444, 738
BlsI GCNGC 5 cut(s) 176, 367, 370, 529, 766
Bme1390I CCNGG 2 cut(s) 227, 244
BmeT110I CYCGRG 1 cut(s) 41
BmgT120I GGNCC 1 cut(s) 405
BmiI GGNNCC 2 cut(s) 160, 429
BmrFI CCNGG 2 cut(s) 227, 244
BmrI ACTGGG 1 cut(s) 498
BmsI GCATC 4 cut(s) 70, 123, 385, 807
BmtI GCTAGC 1 cut(s) 818
BmuI ACTGGG 1 cut(s) 498
BplI GAGNNNNNCTC 2 cut(s) 251, 283
BpmI CTGGAG 3 cut(s) 122, 248, 838
Bpu10I CCTNAGC 1 cut(s) 24
Bpu1102I GCTNAGC 2 cut(s) 444, 738
BpuMI CCSGG 1 cut(s) 244
BsaHI GRCGYC 2 cut(s) 177, 297
BsaJI CCNNGG 5 cut(s) 196, 337, 395, 423, 819
BsaWI WCCGGW 2 cut(s) 92, 149
BsaXI ACNNNNNCTCC 1 cut(s) 29
Bsc4I CCNNNNNNNGG 3 cut(s) 232, 414, 580
Bse118I RCCGGY 2 cut(s) 382, 691
Bse1I ACTGG 1 cut(s) 504
Bse3DI GCAATG 2 cut(s) 438, 774
BseBI CCWGG 1 cut(s) 227
BseDI CCNNGG 5 cut(s) 196, 337, 395, 423, 819
BseGI GGATG 2 cut(s) 376, 639
BseLI CCNNNNNNNGG 3 cut(s) 232, 414, 580
BseMI GCAATG 2 cut(s) 438, 774
BseMII CTCAG 4 cut(s) 38, 157, 435, 557
BseNI ACTGG 1 cut(s) 504
BseRI GAGGAG 4 cut(s) 115, 216, 275, 621
BseXI GCAGC 4 cut(s) 352, 380, 514, 751
BshFI GGCC 8 cut(s) 5, 29, 57, 108, 165, 242, 266, 407
BsiHKAI GWGCWC 1 cut(s) 121
BsiHKCI CYCGRG 1 cut(s) 41
BsiSI CCGG 8 cut(s) 93, 114, 150, 162, 243, 257, 383, 692
BslI CCNNNNNNNGG 3 cut(s) 232, 414, 580
BsmAI GTCTC 2 cut(s) 285, 562
BsmBI CGTCTC 1 cut(s) 285
BsnI GGCC 8 cut(s) 5, 29, 57, 108, 165, 242, 266, 407
BsoBI CYCGRG 1 cut(s) 41
Bsp1286I GDGCHC 1 cut(s) 121
Bsp143I GATC 1 cut(s) 427
Bsp1720I GCTNAGC 2 cut(s) 444, 738
Bsp19I CCATGG 1 cut(s) 337
BspACI CCGC 6 cut(s) 75, 174, 183, 283, 374, 448
BspANI GGCC 8 cut(s) 5, 29, 57, 108, 165, 242, 266, 407
BspCNI CTCAG 4 cut(s) 37, 156, 436, 558
BspLI GGNNCC 2 cut(s) 160, 429
BspMAI CTGCAG 1 cut(s) 370
BspOI GCTAGC 1 cut(s) 818
BspPI GGATC 2 cut(s) 422, 435
BsrBI CCGCTC 1 cut(s) 448
BsrDI GCAATG 2 cut(s) 438, 774
BsrFI RCCGGY 2 cut(s) 382, 691
BsrI ACTGG 1 cut(s) 504
BssAI RCCGGY 2 cut(s) 382, 691
BssECI CCNNGG 5 cut(s) 196, 337, 395, 423, 819
BssMI GATC 1 cut(s) 427
BssNI GRCGYC 2 cut(s) 177, 297
BssT1I CCWWGG 2 cut(s) 337, 395
Bst2UI CCWGG 1 cut(s) 227
Bst4CI ACNGT 1 cut(s) 361
Bst6I CTCTTC 1 cut(s) 406
BstACI GRCGYC 2 cut(s) 177, 297
BstC8I GCNNGC 2 cut(s) 420, 816
BstDEI CTNAG 5 cut(s) 24, 143, 444, 566, 738
BstDSI CCRYGG 3 cut(s) 196, 337, 423
BstENI CCTNNNNNAGG 1 cut(s) 230
BstF5I GGATG 2 cut(s) 376, 639
BstHHI GCGC 1 cut(s) 456
BstKTI GATC 1 cut(s) 430
BstMAI GTCTC 2 cut(s) 285, 562
BstMBI GATC 1 cut(s) 427
BstMWI GCNNNNNNNGC 5 cut(s) 105, 171, 180, 189, 811
BstNI CCWGG 1 cut(s) 227
BstSCI CCNGG 2 cut(s) 225, 242
BstSFI CTRYAG 1 cut(s) 366
BstV1I GCAGC 4 cut(s) 352, 380, 514, 751
BstX2I RGATCY 1 cut(s) 427
BstYI RGATCY 1 cut(s) 427
BsuRI GGCC 8 cut(s) 5, 29, 57, 108, 165, 242, 266, 407
BtgI CCRYGG 3 cut(s) 196, 337, 423
BtsCI GGATG 2 cut(s) 376, 639
BtsIMutI CAGTG 1 cut(s) 511
Cac8I GCNNGC 2 cut(s) 420, 816
CaiI CAGNNNCTG 1 cut(s) 365
CfoI GCGC 1 cut(s) 456
Cfr10I RCCGGY 2 cut(s) 382, 691
Cfr13I GGNCC 1 cut(s) 405
CseI GACGC 1 cut(s) 166
Csp6I GTAC 1 cut(s) 357
CviAII CATG 4 cut(s) 169, 338, 696, 802
CviQI GTAC 1 cut(s) 357
DdeI CTNAG 5 cut(s) 24, 143, 444, 566, 738
DpnI GATC 1 cut(s) 429
DpnII GATC 1 cut(s) 427
EaeI YGGCCR 2 cut(s) 3, 163
Eam1104I CTCTTC 1 cut(s) 406
EarI CTCTTC 1 cut(s) 406
EciI GGCGGA 2 cut(s) 64, 198
Ecl136II GAGCTC 1 cut(s) 119
Eco130I CCWWGG 2 cut(s) 337, 395
Eco147I AGGCCT 2 cut(s) 108, 266
Eco24I GRGCYC 1 cut(s) 121
Eco53kI GAGCTC 1 cut(s) 119
Eco57I CTGAAG 1 cut(s) 636
Eco88I CYCGRG 1 cut(s) 41
EcoICRI GAGCTC 1 cut(s) 119
EcoNI CCTNNNNNAGG 1 cut(s) 230
EcoRII CCWGG 1 cut(s) 225
EcoT14I CCWWGG 2 cut(s) 337, 395
EcoT38I GRGCYC 1 cut(s) 121
ErhI CCWWGG 2 cut(s) 337, 395
Esp3I CGTCTC 1 cut(s) 285
FaeI CATG 4 cut(s) 172, 341, 699, 805
FatI CATG 4 cut(s) 168, 337, 695, 801
Fnu4HI GCNGC 5 cut(s) 175, 366, 369, 528, 765
FokI GGATG 2 cut(s) 363, 626
FriOI GRGCYC 1 cut(s) 121
Fsp4HI GCNGC 5 cut(s) 175, 366, 369, 528, 765
FspBI CTAG 2 cut(s) 497, 815
GlaI GCGC 1 cut(s) 455
GluI GCNGC 5 cut(s) 175, 366, 369, 528, 765
GsuI CTGGAG 3 cut(s) 122, 248, 838
HaeIII GGCC 8 cut(s) 5, 29, 57, 108, 165, 242, 266, 407
HapII CCGG 8 cut(s) 93, 114, 150, 162, 243, 257, 383, 692
HgaI GACGC 1 cut(s) 166
HhaI GCGC 1 cut(s) 456
Hin1I GRCGYC 2 cut(s) 177, 297
Hin1II CATG 4 cut(s) 172, 341, 699, 805
Hin6I GCGC 1 cut(s) 454
HinP1I GCGC 1 cut(s) 454
HindIII AAGCTT 1 cut(s) 740
HinfI GANTC 2 cut(s) 570, 877
HpaII CCGG 8 cut(s) 93, 114, 150, 162, 243, 257, 383, 692
HphI GGTGA 3 cut(s) 164, 412, 683
Hpy166II GTNNAC 1 cut(s) 511
Hpy188I TCNGA 2 cut(s) 19, 871
Hpy188III TCNNGA 2 cut(s) 290, 565
Hpy8I GTNNAC 1 cut(s) 511
Hpy99I CGWCG 5 cut(s) 35, 104, 182, 299, 302
HpyAV CCTTC 4 cut(s) 232, 659, 668, 788
HpyCH4III ACNGT 1 cut(s) 361
HpyCH4IV ACGT 1 cut(s) 297
HpyCH4V TGCA 5 cut(s) 368, 490, 767, 772, 798
HpyF10VI GCNNNNNNNGC 5 cut(s) 105, 171, 180, 189, 811
HpyF3I CTNAG 5 cut(s) 24, 143, 444, 566, 738
HpySE526I ACGT 1 cut(s) 297
Hsp92I GRCGYC 2 cut(s) 177, 297
Hsp92II CATG 4 cut(s) 172, 341, 699, 805
HspAI GCGC 1 cut(s) 454
Kzo9I GATC 1 cut(s) 427
LmnI GCTCC 7 cut(s) 116, 124, 141, 164, 229, 608, 761
Lsp1109I GCAGC 4 cut(s) 352, 380, 514, 751
LweI GCATC 4 cut(s) 70, 123, 385, 807
MaeI CTAG 2 cut(s) 497, 815
MaeII ACGT 1 cut(s) 297
MaeIII GTNAC 2 cut(s) 152, 631
MalI GATC 1 cut(s) 429
MbiI CCGCTC 1 cut(s) 448
MboI GATC 1 cut(s) 427
MboII GAAGA 4 cut(s) 423, 642, 674, 761
MflI RGATCY 1 cut(s) 427
MhlI GDGCHC 1 cut(s) 121
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 48, 64, 85, 348, 477, 825
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 564
MmeI TCCRAC 1 cut(s) 739
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 498
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 8 cut(s) 93, 114, 150, 162, 243, 257, 383, 692
MspR9I CCNGG 2 cut(s) 227, 244
MvaI CCWGG 1 cut(s) 227
MwoI GCNNNNNNNGC 5 cut(s) 105, 171, 180, 189, 811
NciI CCSGG 1 cut(s) 244
NcoI CCATGG 1 cut(s) 337
NdeII GATC 1 cut(s) 427
NheI GCTAGC 1 cut(s) 814
NlaIII CATG 4 cut(s) 172, 341, 699, 805
NlaIV GGNNCC 2 cut(s) 160, 429
NmeAIII GCCGAG 3 cut(s) 33, 482, 800
NmuCI GTSAC 1 cut(s) 152
PaeR7I CTCGAG 1 cut(s) 41
PceI AGGCCT 2 cut(s) 108, 266
PcsI WCGNNNNNNNCGW 2 cut(s) 39, 75
PdmI GAANNNNTTC 1 cut(s) 498
PfeI GAWTC 1 cut(s) 877
PflFI GACNNNGTC 1 cut(s) 296
PkrI GCNGC 5 cut(s) 176, 367, 370, 529, 766
PleI GAGTC 1 cut(s) 564
PpsI GAGTC 1 cut(s) 564
Psp124BI GAGCTC 1 cut(s) 121
Psp6I CCWGG 1 cut(s) 225
PspGI CCWGG 1 cut(s) 225
PspN4I GGNNCC 2 cut(s) 160, 429
PspPI GGNCC 1 cut(s) 405
PspXI VCTCGAGB 1 cut(s) 41
PstI CTGCAG 1 cut(s) 370
PstNI CAGNNNCTG 1 cut(s) 365
PsuI RGATCY 1 cut(s) 427
PsyI GACNNNGTC 1 cut(s) 296
RsaI GTAC 1 cut(s) 358
RsaNI GTAC 1 cut(s) 357
SacI GAGCTC 1 cut(s) 121
SatI GCNGC 5 cut(s) 175, 366, 369, 528, 765
Sau3AI GATC 1 cut(s) 427
Sau96I GGNCC 1 cut(s) 405
SchI GAGTC 1 cut(s) 564
ScrFI CCNGG 2 cut(s) 227, 244
SduI GDGCHC 1 cut(s) 121
SfaNI GCATC 4 cut(s) 70, 123, 385, 807
SfcI CTRYAG 1 cut(s) 366
Sfr274I CTCGAG 1 cut(s) 41
SlaI CTCGAG 1 cut(s) 41
SmlI CTYRAG 1 cut(s) 41
SmoI CTYRAG 1 cut(s) 41
SpeI ACTAGT 1 cut(s) 496
Sse9I AATT 6 cut(s) 48, 64, 85, 348, 477, 825
SseBI AGGCCT 2 cut(s) 108, 266
SsiI CCGC 6 cut(s) 75, 174, 183, 283, 374, 448
SspMI CTAG 2 cut(s) 497, 815
SstI GAGCTC 1 cut(s) 121
StuI AGGCCT 2 cut(s) 108, 266
StyD4I CCNGG 2 cut(s) 225, 242
StyI CCWWGG 2 cut(s) 337, 395
TaaI ACNGT 1 cut(s) 361
TaiI ACGT 1 cut(s) 300
TaqI TCGA 3 cut(s) 42, 128, 289
TasI AATT 6 cut(s) 48, 64, 85, 348, 477, 825
TatI WGTACW 1 cut(s) 356
TauI GCSGC 1 cut(s) 177
TfiI GAWTC 1 cut(s) 877
TscAI CASTG 1 cut(s) 511
TseFI GTSAC 1 cut(s) 152
TseI GCWGC 4 cut(s) 365, 368, 527, 764
Tsp45I GTSAC 1 cut(s) 152
TspDTI ATGAA 2 cut(s) 876, 890
TspGWI ACGGA 4 cut(s) 117, 213, 440, 650
TspRI CASTG 1 cut(s) 511
Tth111I GACNNNGTC 1 cut(s) 296
XagI CCTNNNNNAGG 1 cut(s) 230
XapI RAATTY 1 cut(s) 48
XhoI CTCGAG 1 cut(s) 41
XmnI GAANNNNTTC 1 cut(s) 498
XspI CTAG 2 cut(s) 497, 815
ZraI GACGTC 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.