MD08G1013900.v1.1

HVA22-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0004929
Physical Location & Seq
Forward (+)
1061853 .. 1065046
3194 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1013900.v1.1.491

Sequence Viewer

Length: 885 bp
ATGATAGGATCCTTTCTAACCAGAACACTTGTGATGGCTCTTGGCTATGCTTATCCAGCTTATGAGTGCTACAAAACTGTTGAAAAGAATAAGCCGGAGATTGAGCAGCTTCGCTTTTGGTGCCAGTATTGGATTTTGGTGGCTGTTTTGACTGTATGTGAGAGAGTTGGTGATGCTTTTGTTTCCTGGGTTCCCATGTATAGCGAAGCTAAGTTGCTCTTCATTATATATCTGTGGTTTCCGAAAACAAAGGGAACGACCTATGTGTATGATTCCTTCTTTAGACCATATCTTGCGAAGCATGAAAATGAAATTGATAGGAACTTGTTGGAACTAAAAACTAGAGCTGGTGACATGGCCATTGTGTATTGGCAAAGAGCTGCAAGCTATGGTCAGACGAGAATATTTGATATCTTGCAGTATGTTGCTGCACAATCGACACCAAGGCCTCGCCCTGCTCAGTCACAACAAGGTGTTAGGCGCCCACCCCCTGCTGCTCGCCAACCCACCACACCACCTCCAAATCGCCAGCCAGCTGCAACTACGCAATCACAACCCGAGGAACCACCATCTCCCACTTCCAGTACTTCTTCAAGCAAGGACCAAAAGGAGATAGTGGAAGCCTTAGCACCATCACCAAAGCCTAAACCTGCCCCTCAAACGGCAGGTTCAATTACTCAAAAAGCCACAGCAACAGCAGCTGCATCTGAATCATGTAGCCAAACCACCCCAACTGAAGAGGCTGCAACGACGCAGACTGACGCAGCGCCATCCTCTTCGGCACCTGAAGAGACAAATCCTCCCCCGAAAGAGACGGTCATGGAGGAAACCATTCGGGCCACACGTGGAAAGTTGAGGAAAGCACGCTCTGCAGGAAGCCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TB2_DP1_HVA22

Protein Analysis

295

Amino Acids

32.61

Weight (kDa)

9.16

Isoelectric Point (pI)

65.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TB2_DP1_HVA22 PF03134 19 - 97 4.3e-26 TB2/DP1, HVA22 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 656, 658
AccB1I GGYRCC 3 cut(s) 120, 480, 781
AclWI GGATC 2 cut(s) 3, 16
AcoI YGGCCR 1 cut(s) 357
AcuI CTGAAG 2 cut(s) 756, 807
AcvI CACGTG 1 cut(s) 845
AcyI GRCGYC 1 cut(s) 481
AfaI GTAC 1 cut(s) 586
AfiI CCNNNNNNNGG 1 cut(s) 661
AflIII ACRYGT 1 cut(s) 842
AgsI TTSAA 3 cut(s) 83, 594, 672
AjnI CCWGG 1 cut(s) 185
AluBI AGCT 8 cut(s) 59, 109, 209, 347, 380, 387, 536, 701
AluI AGCT 8 cut(s) 59, 109, 209, 347, 380, 387, 536, 701
Alw26I GTCTC 2 cut(s) 785, 806
AlwI GGATC 2 cut(s) 3, 16
AlwNI CAGNNNCTG 1 cut(s) 701
Ama87I CYCGRG 1 cut(s) 557
AoxI GGCC 3 cut(s) 357, 446, 837
ApeKI GCWGC 9 cut(s) 106, 380, 428, 494, 536, 698, 701, 743, 764
Asp700I GAANNNNTTC 1 cut(s) 831
AspLEI GCGC 2 cut(s) 483, 769
AspS9I GGNCC 2 cut(s) 601, 837
AsuHPI GGTGA 3 cut(s) 182, 362, 627
AvaI CYCGRG 1 cut(s) 557
AvaII GGWCC 1 cut(s) 601
BalI TGGCCA 1 cut(s) 359
BamHI GGATCC 1 cut(s) 8
BanI GGYRCC 3 cut(s) 120, 480, 781
BarI GAAGNNNNNNTAC 2 cut(s) 260, 292
BbrPI CACGTG 1 cut(s) 845
BbvI GCAGC 9 cut(s) 118, 367, 415, 481, 523, 688, 710, 730, 776
BccI CCATC 4 cut(s) 28, 577, 640, 778
BceAI ACGGC 2 cut(s) 678, 864
BciT130I CCWGG 1 cut(s) 187
BcoDI GTCTC 2 cut(s) 785, 806
BfaI CTAG 1 cut(s) 342
BfmI CTRYAG 1 cut(s) 870
BfoI RGCGCY 2 cut(s) 484, 770
BfuAI ACCTGC 2 cut(s) 656, 658
BisI GCNGC 9 cut(s) 107, 381, 429, 495, 537, 699, 702, 744, 765
BlsI GCNGC 9 cut(s) 108, 382, 430, 496, 538, 700, 703, 745, 766
BmcAI AGTACT 1 cut(s) 586
Bme1390I CCNGG 1 cut(s) 187
Bme18I GGWCC 1 cut(s) 601
BmeT110I CYCGRG 1 cut(s) 557
BmgT120I GGNCC 2 cut(s) 601, 837
BmiI GGNNCC 6 cut(s) 10, 122, 192, 482, 564, 783
BmrFI CCNGG 1 cut(s) 187
BmsI GCATC 2 cut(s) 163, 713
Bpu10I CCTNAGC 1 cut(s) 625
BsaAI YACGTR 1 cut(s) 845
BsaHI GRCGYC 1 cut(s) 481
BsaJI CCNNGG 3 cut(s) 186, 443, 558
BsaXI ACNNNNNCTCC 4 cut(s) 502, 532, 784, 814
Bsc4I CCNNNNNNNGG 1 cut(s) 661
Bse1I ACTGG 2 cut(s) 124, 582
BseBI CCWGG 1 cut(s) 187
BseDI CCNNGG 3 cut(s) 186, 443, 558
BseGI GGATG 1 cut(s) 770
BseLI CCNNNNNNNGG 1 cut(s) 661
BseMII CTCAG 1 cut(s) 473
BseNI ACTGG 2 cut(s) 124, 582
BseXI GCAGC 9 cut(s) 118, 367, 415, 481, 523, 688, 710, 730, 776
BsgI GTGCAG 1 cut(s) 414
BshFI GGCC 3 cut(s) 359, 448, 839
BshNI GGYRCC 3 cut(s) 120, 480, 781
BsiHKCI CYCGRG 1 cut(s) 557
BsiSI CCGG 1 cut(s) 95
BslI CCNNNNNNNGG 1 cut(s) 661
BsmAI GTCTC 2 cut(s) 785, 806
BsmBI CGTCTC 1 cut(s) 806
BsnI GGCC 3 cut(s) 359, 448, 839
BsoBI CYCGRG 1 cut(s) 557
Bsp143I GATC 1 cut(s) 8
BspANI GGCC 3 cut(s) 359, 448, 839
BspCNI CTCAG 1 cut(s) 472
BspLI GGNNCC 6 cut(s) 10, 122, 192, 482, 564, 783
BspMAI CTGCAG 1 cut(s) 874
BspMI ACCTGC 2 cut(s) 656, 658
BspPI GGATC 2 cut(s) 3, 16
BspQI GCTCTTC 1 cut(s) 224
BspT107I GGYRCC 3 cut(s) 120, 480, 781
BsrI ACTGG 2 cut(s) 124, 582
BssECI CCNNGG 3 cut(s) 186, 443, 558
BssMI GATC 1 cut(s) 8
BssNI GRCGYC 1 cut(s) 481
BssT1I CCWWGG 1 cut(s) 443
Bst2UI CCWGG 1 cut(s) 187
Bst4CI ACNGT 3 cut(s) 79, 154, 817
Bst6I CTCTTC 4 cut(s) 224, 732, 781, 783
BstACI GRCGYC 1 cut(s) 481
BstAPI GCANNNNNTGC 1 cut(s) 869
BstBAI YACGTR 1 cut(s) 845
BstC8I GCNNGC 5 cut(s) 385, 499, 530, 534, 865
BstDEI CTNAG 3 cut(s) 210, 459, 625
BstF5I GGATG 1 cut(s) 770
BstH2I RGCGCY 2 cut(s) 484, 770
BstHHI GCGC 2 cut(s) 483, 769
BstKTI GATC 1 cut(s) 11
BstMAI GTCTC 2 cut(s) 785, 806
BstMBI GATC 1 cut(s) 8
BstMWI GCNNNNNNNGC 4 cut(s) 56, 120, 698, 869
BstNI CCWGG 1 cut(s) 187
BstSCI CCNGG 1 cut(s) 185
BstSFI CTRYAG 1 cut(s) 870
BstV1I GCAGC 9 cut(s) 118, 367, 415, 481, 523, 688, 710, 730, 776
BstX2I RGATCY 1 cut(s) 8
BstYI RGATCY 1 cut(s) 8
BsuRI GGCC 3 cut(s) 359, 448, 839
BtsCI GGATG 1 cut(s) 770
BveI ACCTGC 2 cut(s) 656, 658
Cac8I GCNNGC 5 cut(s) 385, 499, 530, 534, 865
CaiI CAGNNNCTG 1 cut(s) 701
CfoI GCGC 2 cut(s) 483, 769
Cfr13I GGNCC 2 cut(s) 601, 837
CseI GACGC 2 cut(s) 760, 770
Csp6I GTAC 1 cut(s) 585
CviAII CATG 5 cut(s) 196, 302, 355, 714, 820
CviQI GTAC 1 cut(s) 585
DdeI CTNAG 3 cut(s) 210, 459, 625
DinI GGCGCC 1 cut(s) 482
DpnI GATC 1 cut(s) 10
DpnII GATC 1 cut(s) 8
EaeI YGGCCR 1 cut(s) 357
Eam1104I CTCTTC 4 cut(s) 224, 732, 781, 783
EarI CTCTTC 4 cut(s) 224, 732, 781, 783
Eco130I CCWWGG 1 cut(s) 443
Eco147I AGGCCT 1 cut(s) 448
Eco32I GATATC 1 cut(s) 412
Eco47I GGWCC 1 cut(s) 601
Eco57I CTGAAG 2 cut(s) 756, 807
Eco72I CACGTG 1 cut(s) 845
Eco88I CYCGRG 1 cut(s) 557
EcoRII CCWGG 1 cut(s) 185
EcoRV GATATC 1 cut(s) 412
EcoT14I CCWWGG 1 cut(s) 443
EgeI GGCGCC 1 cut(s) 482
EheI GGCGCC 1 cut(s) 482
ErhI CCWWGG 1 cut(s) 443
Esp3I CGTCTC 1 cut(s) 806
FaeI CATG 5 cut(s) 199, 305, 358, 717, 823
FalI AAGNNNNNCTT 2 cut(s) 203, 235
FatI CATG 5 cut(s) 195, 301, 354, 713, 819
Fnu4HI GCNGC 9 cut(s) 107, 381, 429, 495, 537, 699, 702, 744, 765
FokI GGATG 1 cut(s) 757
Fsp4HI GCNGC 9 cut(s) 107, 381, 429, 495, 537, 699, 702, 744, 765
FspBI CTAG 1 cut(s) 342
GlaI GCGC 2 cut(s) 482, 768
GluI GCNGC 9 cut(s) 107, 381, 429, 495, 537, 699, 702, 744, 765
HaeII RGCGCY 2 cut(s) 484, 770
HaeIII GGCC 3 cut(s) 359, 448, 839
HapII CCGG 1 cut(s) 95
HgaI GACGC 2 cut(s) 760, 770
HhaI GCGC 2 cut(s) 483, 769
Hin1I GRCGYC 1 cut(s) 481
Hin1II CATG 5 cut(s) 199, 305, 358, 717, 823
Hin6I GCGC 2 cut(s) 481, 767
HinP1I GCGC 2 cut(s) 481, 767
HinfI GANTC 2 cut(s) 272, 710
HpaII CCGG 1 cut(s) 95
HphI GGTGA 3 cut(s) 182, 362, 627
Hpy188I TCNGA 3 cut(s) 243, 396, 709
Hpy99I CGWCG 1 cut(s) 754
HpyAV CCTTC 1 cut(s) 286
HpyCH4III ACNGT 3 cut(s) 79, 154, 817
HpyCH4IV ACGT 1 cut(s) 844
HpyCH4V TGCA 7 cut(s) 383, 418, 431, 539, 704, 746, 872
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 120, 698, 869
HpyF3I CTNAG 3 cut(s) 210, 459, 625
HpySE526I ACGT 1 cut(s) 844
Hsp92I GRCGYC 1 cut(s) 481
Hsp92II CATG 5 cut(s) 199, 305, 358, 717, 823
HspAI GCGC 2 cut(s) 481, 767
KasI GGCGCC 1 cut(s) 480
Kzo9I GATC 1 cut(s) 8
LguI GCTCTTC 1 cut(s) 224
Lsp1109I GCAGC 9 cut(s) 118, 367, 415, 481, 523, 688, 710, 730, 776
LweI GCATC 2 cut(s) 163, 713
MaeI CTAG 1 cut(s) 342
MaeII ACGT 1 cut(s) 844
MaeIII GTNAC 2 cut(s) 350, 462
MalI GATC 1 cut(s) 10
MboI GATC 1 cut(s) 8
MboII GAAGA 5 cut(s) 211, 582, 749, 768, 800
MflI RGATCY 1 cut(s) 8
MlsI TGGCCA 1 cut(s) 359
MluCI AATT 2 cut(s) 312, 672
MluNI TGGCCA 1 cut(s) 359
Mly113I GGCGCC 1 cut(s) 481
MmeI TCCRAC 1 cut(s) 309
MnlI CCTC 9 cut(s) 459, 528, 553, 666, 733, 784, 810, 817, 849
Mox20I TGGCCA 1 cut(s) 359
MroXI GAANNNNTTC 1 cut(s) 831
MscI TGGCCA 1 cut(s) 359
MslI CAYNNNNRTG 1 cut(s) 306
Msp20I TGGCCA 1 cut(s) 359
MspA1I CMGCKG 2 cut(s) 536, 701
MspI CCGG 1 cut(s) 95
MspR9I CCNGG 1 cut(s) 187
MvaI CCWGG 1 cut(s) 187
MwoI GCNNNNNNNGC 4 cut(s) 56, 120, 698, 869
NarI GGCGCC 1 cut(s) 481
NdeII GATC 1 cut(s) 8
NlaIII CATG 5 cut(s) 199, 305, 358, 717, 823
NlaIV GGNNCC 6 cut(s) 10, 122, 192, 482, 564, 783
NmuCI GTSAC 2 cut(s) 350, 462
PceI AGGCCT 1 cut(s) 448
PciSI GCTCTTC 1 cut(s) 224
PcsI WCGNNNNNNNCGW 1 cut(s) 841
PdmI GAANNNNTTC 1 cut(s) 831
PfeI GAWTC 2 cut(s) 272, 710
PkrI GCNGC 9 cut(s) 108, 382, 430, 496, 538, 700, 703, 745, 766
PluTI GGCGCC 1 cut(s) 484
PmaCI CACGTG 1 cut(s) 845
PmlI CACGTG 1 cut(s) 845
Ppu21I YACGTR 1 cut(s) 845
Psp6I CCWGG 1 cut(s) 185
PspCI CACGTG 1 cut(s) 845
PspGI CCWGG 1 cut(s) 185
PspN4I GGNNCC 6 cut(s) 10, 122, 192, 482, 564, 783
PspPI GGNCC 2 cut(s) 601, 837
PstI CTGCAG 1 cut(s) 874
PstNI CAGNNNCTG 1 cut(s) 701
PsuI RGATCY 1 cut(s) 8
PvuII CAGCTG 2 cut(s) 536, 701
RsaI GTAC 1 cut(s) 586
RsaNI GTAC 1 cut(s) 585
RseI CAYNNNNRTG 1 cut(s) 306
SapI GCTCTTC 1 cut(s) 224
SatI GCNGC 9 cut(s) 107, 381, 429, 495, 537, 699, 702, 744, 765
Sau3AI GATC 1 cut(s) 8
Sau96I GGNCC 2 cut(s) 601, 837
ScaI AGTACT 1 cut(s) 586
ScrFI CCNGG 1 cut(s) 187
SfaNI GCATC 2 cut(s) 163, 713
SfcI CTRYAG 1 cut(s) 870
SfoI GGCGCC 1 cut(s) 482
SinI GGWCC 1 cut(s) 601
SmiMI CAYNNNNRTG 1 cut(s) 306
Sse9I AATT 2 cut(s) 312, 672
SseBI AGGCCT 1 cut(s) 448
SspDI GGCGCC 1 cut(s) 480
SspI AATATT 1 cut(s) 405
SspMI CTAG 1 cut(s) 342
StuI AGGCCT 1 cut(s) 448
StyD4I CCNGG 1 cut(s) 185
StyI CCWWGG 1 cut(s) 443
TaaI ACNGT 3 cut(s) 79, 154, 817
TaiI ACGT 1 cut(s) 847
TaqI TCGA 1 cut(s) 437
TasI AATT 2 cut(s) 312, 672
TatI WGTACW 1 cut(s) 584
TfiI GAWTC 2 cut(s) 272, 710
TseFI GTSAC 2 cut(s) 350, 462
TseI GCWGC 9 cut(s) 106, 380, 428, 494, 536, 698, 701, 743, 764
Tsp45I GTSAC 2 cut(s) 350, 462
TspDTI ATGAA 3 cut(s) 211, 318, 324
VpaK11BI GGWCC 1 cut(s) 601
XmnI GAANNNNTTC 1 cut(s) 831
XspI CTAG 1 cut(s) 342
ZrmI AGTACT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.