pycom08g01260

HVA22-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0004929
Physical Location & Seq
Forward (+)
940119 .. 941229
1111 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g01260.2

Sequence Viewer

Length: 531 bp
ATGGCCATTGTGTATTGGCAAAGAGCTGCAAGCTATGGTCAGACAAGAATATTTGATATTTTGCAGTATGTTGCTGCACAATCGACACCAAGGCCTCGCCCTGCTCAGCCTCAACAAGGTGTTAGGCGCCCACCCCCTGCTGCTCGCCAACCCACCACACCACCTCCAAATCGCCAGCCAGCTGCAACTACGCAATCACAACCCGAGGAACCGCCATCTCCCACTTCCAGTGCTTCTTCAAGCAAGGACCAAAAGGAGATAGTGGAAGCCTTAGCACCATCACCAAAGCCTAAACCTGCCCCTCAAACGGCAGGTTCAATTACTCAAAAAGCCACAGCAACAGCAGCTGCATCTGAATCATCTAGCCAAACCACCCCAACTGAAGAGGCTGCACCAACGCAGACTGACGCAGCGCCATCCTCTTCGGCAACTGAAGAGACAAATCCTCCCCCGAAAGAGACGGTCATGGAGGAAACCATTCGGGTCACACGTGGAAAGTTGAGGAAAGCACGCTCCGCAGCAAGCCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TB2_DP1_HVA22

Protein Analysis

177

Amino Acids

18.65

Weight (kDa)

9.9

Isoelectric Point (pI)

81.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 302, 304
AccB1I GGYRCC 1 cut(s) 126
AciI CCGC 2 cut(s) 212, 516
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 2 cut(s) 402, 453
AcvI CACGTG 1 cut(s) 491
AcyI GRCGYC 1 cut(s) 127
AfiI CCNNNNNNNGG 2 cut(s) 116, 307
AflIII ACRYGT 1 cut(s) 488
AgsI TTSAA 2 cut(s) 240, 318
AluBI AGCT 4 cut(s) 26, 33, 182, 347
AluI AGCT 4 cut(s) 26, 33, 182, 347
Alw26I GTCTC 2 cut(s) 431, 452
AlwNI CAGNNNCTG 1 cut(s) 347
Ama87I CYCGRG 1 cut(s) 203
AoxI GGCC 2 cut(s) 3, 92
ApeKI GCWGC 9 cut(s) 26, 74, 140, 182, 344, 347, 389, 410, 518
Asp700I GAANNNNTTC 1 cut(s) 477
AspLEI GCGC 2 cut(s) 129, 415
AspS9I GGNCC 1 cut(s) 247
AsuHPI GGTGA 1 cut(s) 273
AvaI CYCGRG 1 cut(s) 203
AvaII GGWCC 1 cut(s) 247
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 126
BbrPI CACGTG 1 cut(s) 491
BbvI GCAGC 8 cut(s) 13, 61, 127, 169, 334, 356, 376, 422
BccI CCATC 3 cut(s) 223, 286, 424
BceAI ACGGC 2 cut(s) 324, 510
BcoDI GTCTC 2 cut(s) 431, 452
BfaI CTAG 1 cut(s) 363
BfoI RGCGCY 2 cut(s) 130, 416
BfuAI ACCTGC 2 cut(s) 302, 304
BisI GCNGC 9 cut(s) 27, 75, 141, 183, 345, 348, 390, 411, 519
BlpI GCTNAGC 1 cut(s) 105
BlsI GCNGC 9 cut(s) 28, 76, 142, 184, 346, 349, 391, 412, 520
Bme18I GGWCC 1 cut(s) 247
BmeT110I CYCGRG 1 cut(s) 203
BmgT120I GGNCC 1 cut(s) 247
BmiI GGNNCC 2 cut(s) 128, 210
BmsI GCATC 1 cut(s) 359
Bpu10I CCTNAGC 1 cut(s) 271
Bpu1102I GCTNAGC 1 cut(s) 105
BsaAI YACGTR 1 cut(s) 491
BsaHI GRCGYC 1 cut(s) 127
BsaJI CCNNGG 2 cut(s) 89, 204
BsaXI ACNNNNNCTCC 4 cut(s) 148, 178, 430, 460
Bsc4I CCNNNNNNNGG 2 cut(s) 116, 307
Bse1I ACTGG 1 cut(s) 228
BseDI CCNNGG 2 cut(s) 89, 204
BseGI GGATG 1 cut(s) 416
BseLI CCNNNNNNNGG 2 cut(s) 116, 307
BseMII CTCAG 1 cut(s) 119
BseNI ACTGG 1 cut(s) 228
BseXI GCAGC 8 cut(s) 13, 61, 127, 169, 334, 356, 376, 422
BsgI GTGCAG 2 cut(s) 60, 375
BshFI GGCC 2 cut(s) 5, 94
BshNI GGYRCC 1 cut(s) 126
BsiHKCI CYCGRG 1 cut(s) 203
BslI CCNNNNNNNGG 2 cut(s) 116, 307
BsmAI GTCTC 2 cut(s) 431, 452
BsmBI CGTCTC 1 cut(s) 452
BsnI GGCC 2 cut(s) 5, 94
BsoBI CYCGRG 1 cut(s) 203
Bsp1720I GCTNAGC 1 cut(s) 105
BspACI CCGC 2 cut(s) 212, 516
BspANI GGCC 2 cut(s) 5, 94
BspCNI CTCAG 1 cut(s) 118
BspLI GGNNCC 2 cut(s) 128, 210
BspMI ACCTGC 2 cut(s) 302, 304
BspT107I GGYRCC 1 cut(s) 126
BsrI ACTGG 1 cut(s) 228
BssECI CCNNGG 2 cut(s) 89, 204
BssNI GRCGYC 1 cut(s) 127
BssT1I CCWWGG 1 cut(s) 89
Bst4CI ACNGT 1 cut(s) 463
Bst6I CTCTTC 3 cut(s) 378, 427, 429
BstACI GRCGYC 1 cut(s) 127
BstBAI YACGTR 1 cut(s) 491
BstC8I GCNNGC 6 cut(s) 31, 145, 176, 180, 511, 523
BstDEI CTNAG 2 cut(s) 105, 271
BstENI CCTNNNNNAGG 1 cut(s) 114
BstF5I GGATG 1 cut(s) 416
BstH2I RGCGCY 2 cut(s) 130, 416
BstHHI GCGC 2 cut(s) 129, 415
BstMAI GTCTC 2 cut(s) 431, 452
BstMWI GCNNNNNNNGC 2 cut(s) 344, 515
BstV1I GCAGC 8 cut(s) 13, 61, 127, 169, 334, 356, 376, 422
BsuRI GGCC 2 cut(s) 5, 94
BtsCI GGATG 1 cut(s) 416
BtsIMutI CAGTG 1 cut(s) 235
BveI ACCTGC 2 cut(s) 302, 304
Cac8I GCNNGC 6 cut(s) 31, 145, 176, 180, 511, 523
CaiI CAGNNNCTG 1 cut(s) 347
CfoI GCGC 2 cut(s) 129, 415
Cfr13I GGNCC 1 cut(s) 247
CseI GACGC 1 cut(s) 416
CviAII CATG 1 cut(s) 466
DdeI CTNAG 2 cut(s) 105, 271
DinI GGCGCC 1 cut(s) 128
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 378, 427, 429
EarI CTCTTC 3 cut(s) 378, 427, 429
Eco130I CCWWGG 1 cut(s) 89
Eco147I AGGCCT 1 cut(s) 94
Eco47I GGWCC 1 cut(s) 247
Eco57I CTGAAG 2 cut(s) 402, 453
Eco72I CACGTG 1 cut(s) 491
Eco88I CYCGRG 1 cut(s) 203
EcoNI CCTNNNNNAGG 1 cut(s) 114
EcoT14I CCWWGG 1 cut(s) 89
EgeI GGCGCC 1 cut(s) 128
EheI GGCGCC 1 cut(s) 128
ErhI CCWWGG 1 cut(s) 89
Esp3I CGTCTC 1 cut(s) 452
FaeI CATG 1 cut(s) 469
FaiI YATR 3 cut(s) 36, 69, 467
FatI CATG 1 cut(s) 465
Fnu4HI GCNGC 9 cut(s) 27, 75, 141, 183, 345, 348, 390, 411, 519
FokI GGATG 1 cut(s) 403
Fsp4HI GCNGC 9 cut(s) 27, 75, 141, 183, 345, 348, 390, 411, 519
FspBI CTAG 1 cut(s) 363
GlaI GCGC 2 cut(s) 128, 414
GluI GCNGC 9 cut(s) 27, 75, 141, 183, 345, 348, 390, 411, 519
HaeII RGCGCY 2 cut(s) 130, 416
HaeIII GGCC 2 cut(s) 5, 94
HgaI GACGC 1 cut(s) 416
HhaI GCGC 2 cut(s) 129, 415
Hin1I GRCGYC 1 cut(s) 127
Hin1II CATG 1 cut(s) 469
Hin6I GCGC 2 cut(s) 127, 413
HinP1I GCGC 2 cut(s) 127, 413
HinfI GANTC 1 cut(s) 356
HphI GGTGA 1 cut(s) 273
Hpy188I TCNGA 2 cut(s) 42, 355
HpyCH4III ACNGT 1 cut(s) 463
HpyCH4IV ACGT 1 cut(s) 490
HpyCH4V TGCA 6 cut(s) 29, 64, 77, 185, 350, 392
HpyF10VI GCNNNNNNNGC 2 cut(s) 344, 515
HpyF3I CTNAG 2 cut(s) 105, 271
HpySE526I ACGT 1 cut(s) 490
Hsp92I GRCGYC 1 cut(s) 127
Hsp92II CATG 1 cut(s) 469
HspAI GCGC 2 cut(s) 127, 413
KasI GGCGCC 1 cut(s) 126
LmnI GCTCC 1 cut(s) 518
LpnPI CCDG 7 cut(s) 114, 150, 188, 192, 241, 297, 309
Lsp1109I GCAGC 8 cut(s) 13, 61, 127, 169, 334, 356, 376, 422
LweI GCATC 1 cut(s) 359
MaeI CTAG 1 cut(s) 363
MaeII ACGT 1 cut(s) 490
MaeIII GTNAC 1 cut(s) 484
MboII GAAGA 4 cut(s) 228, 395, 414, 446
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 318
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 127
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 477
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 182, 347
MwoI GCNNNNNNNGC 2 cut(s) 344, 515
NarI GGCGCC 1 cut(s) 127
NlaIII CATG 1 cut(s) 469
NlaIV GGNNCC 2 cut(s) 128, 210
NmuCI GTSAC 1 cut(s) 484
PceI AGGCCT 1 cut(s) 94
PcsI WCGNNNNNNNCGW 1 cut(s) 487
PdmI GAANNNNTTC 1 cut(s) 477
PfeI GAWTC 1 cut(s) 356
PkrI GCNGC 9 cut(s) 28, 76, 142, 184, 346, 349, 391, 412, 520
PluTI GGCGCC 1 cut(s) 130
PmaCI CACGTG 1 cut(s) 491
PmlI CACGTG 1 cut(s) 491
Ppu21I YACGTR 1 cut(s) 491
PspCI CACGTG 1 cut(s) 491
PspN4I GGNNCC 2 cut(s) 128, 210
PspPI GGNCC 1 cut(s) 247
PstNI CAGNNNCTG 1 cut(s) 347
PvuII CAGCTG 2 cut(s) 182, 347
SatI GCNGC 9 cut(s) 27, 75, 141, 183, 345, 348, 390, 411, 519
Sau96I GGNCC 1 cut(s) 247
SetI ASST 9 cut(s) 28, 35, 121, 166, 184, 298, 316, 349, 493
SfaNI GCATC 1 cut(s) 359
SfoI GGCGCC 1 cut(s) 128
SinI GGWCC 1 cut(s) 247
Sse9I AATT 1 cut(s) 318
SseBI AGGCCT 1 cut(s) 94
SsiI CCGC 2 cut(s) 212, 516
SspDI GGCGCC 1 cut(s) 126
SspI AATATT 1 cut(s) 51
SspMI CTAG 1 cut(s) 363
StuI AGGCCT 1 cut(s) 94
StyI CCWWGG 1 cut(s) 89
TaaI ACNGT 1 cut(s) 463
TaiI ACGT 1 cut(s) 493
TaqI TCGA 1 cut(s) 83
TasI AATT 1 cut(s) 318
TfiI GAWTC 1 cut(s) 356
TscAI CASTG 1 cut(s) 235
TseFI GTSAC 1 cut(s) 484
TseI GCWGC 9 cut(s) 26, 74, 140, 182, 344, 347, 389, 410, 518
Tsp45I GTSAC 1 cut(s) 484
TspRI CASTG 1 cut(s) 235
VpaK11BI GGWCC 1 cut(s) 247
XagI CCTNNNNNAGG 1 cut(s) 114
XmnI GAANNNNTTC 1 cut(s) 477
XspI CTAG 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.