Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0002097

Physical Location & Seq

Genomic Coordinates Forward (+)

27957010 .. 27960930

Gene Structure 3921 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1233200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1743 bp

ATGGTAGGGCAATCAGGAAAATGGATGATACTTGTGGCGGCAACGTGGATACAGGCATTCACAGGGACAAACTTTGATTTCGCATCGTATTCTTCTGAACTGAAATCTGTGCTGGGGATTTCTCAGGTGCAGCTCAACTATCTATCGGTGGCCTCGGATATGGGGAAGGCATTGGGTTGGTGTTCTGGGGTTTCTCTCATGTATTTTCCCTTGTGGATTGTTATGTTCATGGCTGCCTTCATGGGTTTGGTTGGTTATGGCCTCCAATGGTTTGTCATTCGAGGAATCATCTCTCTGCCCTATGTCATGGTATTCCTTCTATGCTTGTTGGCTGGATCCGGCATCTGCTGGTTCAACACAGTCTGCTATGTTCTATGCATCAGACACTTCCAAGCCAACCGCGCACTCGCGTTGTCCCTTTCAATCAGTTACAATGGGATAAGTGCAGCCCTCTACACCCTCATTGCCAATGCAATAAACCCTAACAGTGATACCATCTACCTCCTCCTTAATGCTGTAGTTCCTCTCTTCCCATCCGTCGTTATATTCATCCCTGTGCTCCGTCAGCCCCCAGTTCAGCCCCTTCCCGCTGAGGCCACTCGCCGTGACTCCATGATTTTTCTTTGCCTATACATCCTAGCTGTGGTCACAGGCCTATATCTCCTCCTCCTCAACTCACTATCATCTGATGCGTCAAAAGCTCGGATACTCTTAGTTGGTGCGATTTGTCTCCTAATCCTACCTCTGTCTCTGCCTGGAATGGCATATGCGCGTGAGTGGGCTCGTAGGAACTCCCCCTCAAGCTTCCTTTCTGAAATCTCGTCAACCTTCAATCTGGTTGACACTGATGATCTTGAGCTCCATAAGGAACTCATTGCTGGAAGTGAAAATGATAATGCCACGAATGCCACAAATCCCACCAACACCAGTTCGTATGGGCTGATACACAGGGAAGGCTTTTTTGGGTGTTTCGCTTGTTTTGGGAAGGTGATGGGAAAAGACAGGTTGACAGTGCTAGGTGAAGAGCACTCAGCTAGGCTGCTCGTACGCCGGTGGGATTTTTGGCTATACTATGCTGCATACTTATGTGGGGCAACTATTGGGTTGGTCTACAGCAACAATCTAGGCCAGATTTCAGAATCACTTGGATACAGTTCCCTGACTAGTTCTCTTGTCACAGTATATTCTTCATGCTCCTTCTTCGGCCGTTTGCTCTCAGCTGCACCAGATTTCCTGCGTGACAAGGTCTATTTTGCAAGGACTGGATGGCTAGTGGTTGCTCTGGTGCCAACACCAATTGCCTTCTTTCTGCTGGCCGCATCAGGCAGTGAGGCAATGCTGCGTACCGGAACAGGCTTAATCGGGATAAGCTCTGGCTTTGTGTTTTCTGCAGCTGTGTCAGTCACATCAGAGCTTTTCGGCCCAAACAGCGCCGGTGTAAACCACAACATCCTCATCACCAACATCCCAATTGGCTCACTCCTATATGGCCTTCTTGCAGCTTTGGTATATGATTCCAATGAAGGAAGCTCAACCACCCCAGTGAGCTTGTTAAGGGATGCAACATTGTGCATGGGAAGGTCATGCTATAGGCAGACATTCATATGGTGGGGCTGTATTTCAATAGTAGGTTTAACTTCAAGCTTCTTGCTATTCCTACGGACAAGGACCGCTTATAATCGGTTTGAGAGGAACAGAAATCAGACACAAGTCACAGAATCCTCCTTACAAACCTCCTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Nodulin-like

Protein Analysis

581

Amino Acids

63.35

Weight (kDa)

8.32

Isoelectric Point (pI)

36.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nodulin-like	PF06813	7 - 252	2e-82	Nodulin-like
NFD4_C	PF23262	341 - 558	2.5e-50	NFD4, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002097)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G19450 AT4G19450 AT5G45275
fragaria_vesca	FvH4_5g24410 FvH4_5g24410 FvH4_5g24410 FvH4_5g24410 FvH4_7g07730 FvH4_7g07730 FvH4_7g07730 FvH4_7g07730 FvH4_7g07730
malus_domestica	MD02G1233200.v1.1 MD06G1022100.v1.1 MD07G1080400.v1.1 MD16G1287800.v1.1
prunus_persica	Prupe.2G104800_v2.0.a1 Prupe.5G016300_v2.0.a1
pyrus_communis	pycom06g01600 pycom07g06400
rosa_chinensis	RchiOBHm_Chr1g0342361 RchiOBHm_Chr7g0214811
rosa_laevigata	RLG00000002719 RLG00000002720 RLG00000029156
rosa_multiflora	Rmu_co8462433.1_g000001 Rmu_sc0002308.1_g000032 Rmu_sc0012155.1_g000010
rosa_roxburghii	Rroxscaffold_3G00245010 Rroxscaffold_4G00312980
rosa_rugosa	Rorug01G0151600.1 Rorug07G0147200 Rorug07G0147300 Rorug07G0147400
rosa_samantha	Rh1AG166000 Rh1BG132500 Rh1CG154600 Rh1DG167400 Rh7AG284200 Rh7BG275100 Rh7CG303600 Rh7DG287500
rosa_wichuraiana	Rw1G013870 Rw7G024250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1677
AccB1I	GGYRCC	1 cut(s)	1285
AccI	GTMKAC	1 cut(s)	1110
AccII	CGCG	3 cut(s)	402, 410, 772
AciI	CCGC	5 cut(s)	38, 400, 588, 1317, 1671
AclWI	GGATC	2 cut(s)	330, 343
AcoI	YGGCCR	2 cut(s)	1204, 1314
AfaI	GTAC	2 cut(s)	1047, 1345
AfiI	CCNNNNNNNGG	1 cut(s)	643
AgsI	TTSAA	5 cut(s)	355, 423, 832, 1623, 1641
AhlI	ACTAGT	1 cut(s)	1163
AjnI	CCWGG	1 cut(s)	754
AjuI	GAANNNNNNNTTGG	2 cut(s)	945, 977
AleI	CACNNNNGTG	1 cut(s)	1541
Alw21I	GWGCWC	3 cut(s)	561, 861, 1029
Alw26I	GTCTC	2 cut(s)	734, 753
AlwI	GGATC	2 cut(s)	330, 343
AoxI	GGCC	9 cut(s)	150, 259, 594, 652, 1126, 1204, 1314, 1420, 1489
ApeKI	GCWGC	9 cut(s)	130, 233, 446, 1039, 1076, 1220, 1339, 1391, 1499
AspLEI	GCGC	3 cut(s)	404, 772, 1433
AspS9I	GGNCC	2 cut(s)	1421, 1668
AsuHPI	GGTGA	3 cut(s)	1000, 1031, 1450
AvaII	GGWCC	1 cut(s)	1668
BamHI	GGATCC	1 cut(s)	335
BanI	GGYRCC	1 cut(s)	1285
BanII	GRGCYC	2 cut(s)	784, 861
Bbv12I	GWGCWC	3 cut(s)	561, 861, 1029
BbvCI	CCTCAGC	1 cut(s)	591
BbvI	GCAGC	9 cut(s)	142, 220, 458, 1026, 1063, 1207, 1326, 1403, 1511
BccI	CCATC	4 cut(s)	503, 541, 985, 1260
BceAI	ACGGC	2 cut(s)	588, 1191
BciT130I	CCWGG	1 cut(s)	756
BciVI	GTATCC	3 cut(s)	42, 699, 1142
BcoDI	GTCTC	2 cut(s)	734, 753
BcuI	ACTAGT	1 cut(s)	1163
BfaI	CTAG	6 cut(s)	638, 1016, 1035, 1124, 1164, 1271
BfmI	CTRYAG	4 cut(s)	516, 1111, 1389, 1588
BfoI	RGCGCY	1 cut(s)	1434
BfuI	GTATCC	3 cut(s)	42, 699, 1142
Bme1390I	CCNGG	1 cut(s)	756
Bme18I	GGWCC	1 cut(s)	1668
BmgT120I	GGNCC	2 cut(s)	1421, 1668
BmiI	GGNNCC	2 cut(s)	337, 1287
BmrFI	CCNGG	1 cut(s)	756
BmrI	ACTGGG	2 cut(s)	566, 1535
BmsI	GCATC	6 cut(s)	92, 351, 387, 679, 1328, 1549
BmuI	ACTGGG	2 cut(s)	566, 1535
BoxI	GACNNNNGTC	1 cut(s)	1709
Bpu10I	CCTNAGC	1 cut(s)	591
BpuEI	CTTGAG	2 cut(s)	784, 875
BsaJI	CCNNGG	1 cut(s)	153
BsaWI	WCCGGW	1 cut(s)	1346
Bsc4I	CCNNNNNNNGG	1 cut(s)	643
Bse118I	RCCGGY	2 cut(s)	1050, 1433
Bse1I	ACTGG	4 cut(s)	572, 927, 1267, 1541
Bse3DI	GCAATG	3 cut(s)	462, 873, 1341
BseBI	CCWGG	1 cut(s)	756
BseDI	CCNNGG	1 cut(s)	153
BseGI	GGATG	8 cut(s)	30, 533, 549, 633, 1271, 1449, 1464, 1564
BseLI	CCNNNNNNNGG	1 cut(s)	643
BseMI	GCAATG	3 cut(s)	462, 873, 1341
BseMII	CTCAG	4 cut(s)	137, 582, 1044, 1230
BseNI	ACTGG	4 cut(s)	572, 927, 1267, 1541
BseRI	GAGGAG	5 cut(s)	494, 653, 656, 659, 1726
BseX3I	CGGCCG	1 cut(s)	1204
BseXI	GCAGC	9 cut(s)	142, 220, 458, 1026, 1063, 1207, 1326, 1403, 1511
BseYI	CCCAGC	1 cut(s)	112
BsgI	GTGCAG	3 cut(s)	149, 465, 1206
Bsh1236I	CGCG	3 cut(s)	402, 410, 772
Bsh1285I	CGRYCG	1 cut(s)	1207
BshFI	GGCC	9 cut(s)	152, 261, 596, 654, 1128, 1206, 1316, 1422, 1491
BshNI	GGYRCC	1 cut(s)	1285
BsiEI	CGRYCG	1 cut(s)	1207
BsiHKAI	GWGCWC	3 cut(s)	561, 861, 1029
BsiSI	CCGG	4 cut(s)	339, 1051, 1347, 1434
BsiWI	CGTACG	1 cut(s)	1045
BslFI	GGGAC	2 cut(s)	79, 400
BslI	CCNNNNNNNGG	1 cut(s)	643
BsmAI	GTCTC	2 cut(s)	734, 753
BsmFI	GGGAC	2 cut(s)	79, 400
BsmI	GAATGC	2 cut(s)	56, 910
BsnI	GGCC	9 cut(s)	152, 261, 596, 654, 1128, 1206, 1316, 1422, 1491
Bsp1286I	GDGCHC	4 cut(s)	561, 784, 861, 1029
Bsp143I	GATC	2 cut(s)	335, 850
BspACI	CCGC	5 cut(s)	38, 400, 588, 1317, 1671
BspANI	GGCC	9 cut(s)	152, 261, 596, 654, 1128, 1206, 1316, 1422, 1491
BspCNI	CTCAG	4 cut(s)	136, 583, 1043, 1229
BspFNI	CGCG	3 cut(s)	402, 410, 772
BspLI	GGNNCC	2 cut(s)	337, 1287
BspMAI	CTGCAG	1 cut(s)	1393
BspPI	GGATC	2 cut(s)	330, 343
BspQI	GCTCTTC	1 cut(s)	1017
BspT107I	GGYRCC	1 cut(s)	1285
BsrDI	GCAATG	3 cut(s)	462, 873, 1341
BsrFI	RCCGGY	2 cut(s)	1050, 1433
BsrI	ACTGG	4 cut(s)	572, 927, 1267, 1541
BssAI	RCCGGY	2 cut(s)	1050, 1433
BssECI	CCNNGG	1 cut(s)	153
BssMI	GATC	2 cut(s)	335, 850
Bst2UI	CCWGG	1 cut(s)	756
Bst4CI	ACNGT	5 cut(s)	361, 488, 1012, 1154, 1180
Bst6I	CTCTTC	2 cut(s)	533, 1017
BstC8I	GCNNGC	1 cut(s)	1314
BstDEI	CTNAG	6 cut(s)	123, 591, 712, 1030, 1216, 1740
BstF5I	GGATG	8 cut(s)	30, 533, 549, 633, 1271, 1449, 1464, 1564
BstFNI	CGCG	3 cut(s)	402, 410, 772
BstH2I	RGCGCY	1 cut(s)	1434
BstHHI	GCGC	3 cut(s)	404, 772, 1433
BstKTI	GATC	2 cut(s)	338, 853
BstMAI	GTCTC	2 cut(s)	734, 753
BstMBI	GATC	2 cut(s)	335, 850
BstMCI	CGRYCG	1 cut(s)	1207
BstMWI	GCNNNNNNNGC	5 cut(s)	401, 565, 698, 905, 1428
BstNI	CCWGG	1 cut(s)	756
BstPAI	GACNNNNGTC	1 cut(s)	1709
BstSCI	CCNGG	1 cut(s)	754
BstSFI	CTRYAG	4 cut(s)	516, 1111, 1389, 1588
BstUI	CGCG	3 cut(s)	402, 410, 772
BstV1I	GCAGC	9 cut(s)	142, 220, 458, 1026, 1063, 1207, 1326, 1403, 1511
BstX2I	RGATCY	1 cut(s)	335
BstYI	RGATCY	1 cut(s)	335
BstZI	CGGCCG	1 cut(s)	1204
BsuI	GTATCC	3 cut(s)	42, 699, 1142
BsuRI	GGCC	9 cut(s)	152, 261, 596, 654, 1128, 1206, 1316, 1422, 1491
BtsCI	GGATG	8 cut(s)	30, 533, 549, 633, 1271, 1449, 1464, 1564
BtsI	GCAGTG	1 cut(s)	1333
BtsIMutI	CAGTG	5 cut(s)	493, 843, 1017, 1333, 1548
Cac8I	GCNNGC	1 cut(s)	1314
CfoI	GCGC	3 cut(s)	404, 772, 1433
Cfr10I	RCCGGY	2 cut(s)	1050, 1433
Cfr13I	GGNCC	2 cut(s)	1421, 1668
CseI	GACGC	1 cut(s)	681
Csp6I	GTAC	2 cut(s)	1046, 1344
CviAII	CATG	8 cut(s)	199, 229, 241, 307, 613, 1191, 1573, 1584
CviQI	GTAC	2 cut(s)	1046, 1344
DdeI	CTNAG	6 cut(s)	123, 591, 712, 1030, 1216, 1740
DpnI	GATC	2 cut(s)	337, 852
DpnII	GATC	2 cut(s)	335, 850
EaeI	YGGCCR	2 cut(s)	1204, 1314
EagI	CGGCCG	1 cut(s)	1204
Eam1104I	CTCTTC	2 cut(s)	533, 1017
EarI	CTCTTC	2 cut(s)	533, 1017
Ecl136II	GAGCTC	1 cut(s)	859
EclXI	CGGCCG	1 cut(s)	1204
Eco147I	AGGCCT	1 cut(s)	654
Eco24I	GRGCYC	2 cut(s)	784, 861
Eco47I	GGWCC	1 cut(s)	1668
Eco52I	CGGCCG	1 cut(s)	1204
Eco53kI	GAGCTC	1 cut(s)	859
EcoICRI	GAGCTC	1 cut(s)	859
EcoRII	CCWGG	1 cut(s)	754
EcoT22I	ATGCAT	1 cut(s)	380
EcoT38I	GRGCYC	2 cut(s)	784, 861
FaeI	CATG	8 cut(s)	202, 232, 244, 310, 616, 1194, 1576, 1587
FalI	AAGNNNNNCTT	2 cut(s)	1657, 1689
FaqI	GGGAC	2 cut(s)	79, 400
FatI	CATG	8 cut(s)	198, 228, 240, 306, 612, 1190, 1572, 1583
FauI	CCCGC	1 cut(s)	595
FauNDI	CATATG	2 cut(s)	766, 1604
FblI	GTMKAC	1 cut(s)	1110
FokI	GGATG	8 cut(s)	37, 520, 536, 620, 1278, 1436, 1451, 1571
FriOI	GRGCYC	2 cut(s)	784, 861
FspBI	CTAG	6 cut(s)	638, 1016, 1035, 1124, 1164, 1271
GlaI	GCGC	3 cut(s)	403, 771, 1432
GsaI	CCCAGC	1 cut(s)	116
HaeII	RGCGCY	1 cut(s)	1434
HaeIII	GGCC	9 cut(s)	152, 261, 596, 654, 1128, 1206, 1316, 1422, 1491
HapII	CCGG	4 cut(s)	339, 1051, 1347, 1434
HgaI	GACGC	1 cut(s)	681
HhaI	GCGC	3 cut(s)	404, 772, 1433
Hin1II	CATG	8 cut(s)	202, 232, 244, 310, 616, 1194, 1576, 1587
Hin6I	GCGC	3 cut(s)	402, 770, 1431
HinP1I	GCGC	3 cut(s)	402, 770, 1431
HincII	GTYRAC	3 cut(s)	825, 841, 1008
HindII	GTYRAC	3 cut(s)	825, 841, 1008
HindIII	AAGCTT	2 cut(s)	802, 1642
HinfI	GANTC	5 cut(s)	285, 608, 1139, 1514, 1718
HpaII	CCGG	4 cut(s)	339, 1051, 1347, 1434
HphI	GGTGA	3 cut(s)	1000, 1031, 1450
Hpy166II	GTNNAC	5 cut(s)	825, 841, 1008, 1111, 1441
Hpy188I	TCNGA	9 cut(s)	97, 157, 383, 688, 705, 814, 1138, 1411, 1704
Hpy188III	TCNNGA	3 cut(s)	15, 854, 1363
Hpy8I	GTNNAC	5 cut(s)	825, 841, 1008, 1111, 1441
Hpy99I	CGWCG	1 cut(s)	542
HpyCH4III	ACNGT	5 cut(s)	361, 488, 1012, 1154, 1180
HpyCH4IV	ACGT	1 cut(s)	44
HpyF10VI	GCNNNNNNNGC	5 cut(s)	401, 565, 698, 905, 1428
HpyF3I	CTNAG	6 cut(s)	123, 591, 712, 1030, 1216, 1740
HpySE526I	ACGT	1 cut(s)	44
Hsp92II	CATG	8 cut(s)	202, 232, 244, 310, 616, 1194, 1576, 1587
HspAI	GCGC	3 cut(s)	402, 770, 1431
Kzo9I	GATC	2 cut(s)	335, 850
LguI	GCTCTTC	1 cut(s)	1017
LmnI	GCTCC	3 cut(s)	564, 864, 1199
Lsp1109I	GCAGC	9 cut(s)	142, 220, 458, 1026, 1063, 1207, 1326, 1403, 1511
LweI	GCATC	6 cut(s)	92, 351, 387, 679, 1328, 1549
MaeI	CTAG	6 cut(s)	638, 1016, 1035, 1124, 1164, 1271
MaeII	ACGT	1 cut(s)	44
MaeIII	GTNAC	7 cut(s)	428, 605, 646, 1174, 1238, 1402, 1711
MalI	GATC	2 cut(s)	337, 852
MboI	GATC	2 cut(s)	335, 850
MboII	GAAGA	5 cut(s)	84, 520, 1034, 1179, 1192
MfeI	CAATTG	2 cut(s)	1296, 1470
MflI	RGATCY	1 cut(s)	335
MhlI	GDGCHC	4 cut(s)	561, 784, 861, 1029
MluCI	AATT	2 cut(s)	1296, 1470
MlyI	GAGTC	1 cut(s)	602
Mph1103I	ATGCAT	1 cut(s)	380
MseI	TTAA	4 cut(s)	510, 1358, 1553, 1634
MslI	CAYNNNNRTG	4 cut(s)	554, 1084, 1541, 1603
MspA1I	CMGCKG	3 cut(s)	590, 1220, 1394
MspI	CCGG	4 cut(s)	339, 1051, 1347, 1434
MspR9I	CCNGG	1 cut(s)	756
MunI	CAATTG	2 cut(s)	1296, 1470
Mva1269I	GAATGC	2 cut(s)	56, 910
MvaI	CCWGG	1 cut(s)	756
MvnI	CGCG	3 cut(s)	402, 410, 772
MwoI	GCNNNNNNNGC	5 cut(s)	401, 565, 698, 905, 1428
NdeI	CATATG	2 cut(s)	766, 1604
NdeII	GATC	2 cut(s)	335, 850
NlaIII	CATG	8 cut(s)	202, 232, 244, 310, 616, 1194, 1576, 1587
NlaIV	GGNNCC	2 cut(s)	337, 1287
NmuCI	GTSAC	6 cut(s)	605, 646, 1174, 1238, 1402, 1711
NsiI	ATGCAT	1 cut(s)	380
OliI	CACNNNNGTG	1 cut(s)	1541
PceI	AGGCCT	1 cut(s)	654
PciSI	GCTCTTC	1 cut(s)	1017
PctI	GAATGC	2 cut(s)	56, 910
PfeI	GAWTC	4 cut(s)	285, 1139, 1514, 1718
Pfl23II	CGTACG	1 cut(s)	1045
PflFI	GACNNNGTC	1 cut(s)	1244
PleI	GAGTC	1 cut(s)	602
PpsI	GAGTC	1 cut(s)	602
PshAI	GACNNNNGTC	1 cut(s)	1709
PsiI	TTATAA	1 cut(s)	1677
Psp124BI	GAGCTC	1 cut(s)	861
Psp6I	CCWGG	1 cut(s)	754
PspFI	CCCAGC	1 cut(s)	112
PspGI	CCWGG	1 cut(s)	754
PspLI	CGTACG	1 cut(s)	1045
PspN4I	GGNNCC	2 cut(s)	337, 1287
PspPI	GGNCC	2 cut(s)	1421, 1668
PstI	CTGCAG	1 cut(s)	1393
PsuI	RGATCY	1 cut(s)	335
PsyI	GACNNNGTC	1 cut(s)	1244
PvuII	CAGCTG	2 cut(s)	1220, 1394
RsaI	GTAC	2 cut(s)	1047, 1345
RsaNI	GTAC	2 cut(s)	1046, 1344
RseI	CAYNNNNRTG	4 cut(s)	554, 1084, 1541, 1603
SacI	GAGCTC	1 cut(s)	861
SapI	GCTCTTC	1 cut(s)	1017
SaqAI	TTAA	4 cut(s)	510, 1358, 1553, 1634
Sau3AI	GATC	2 cut(s)	335, 850
Sau96I	GGNCC	2 cut(s)	1421, 1668
SchI	GAGTC	1 cut(s)	602
ScrFI	CCNGG	1 cut(s)	756
SduI	GDGCHC	4 cut(s)	561, 784, 861, 1029
SfaNI	GCATC	6 cut(s)	92, 351, 387, 679, 1328, 1549
SfcI	CTRYAG	4 cut(s)	516, 1111, 1389, 1588
SgrAI	CRCCGGYG	2 cut(s)	1050, 1433
SinI	GGWCC	1 cut(s)	1668
SmiMI	CAYNNNNRTG	4 cut(s)	554, 1084, 1541, 1603
SmlI	CTYRAG	2 cut(s)	799, 854
SmoI	CTYRAG	2 cut(s)	799, 854
SpeI	ACTAGT	1 cut(s)	1163
Sse9I	AATT	2 cut(s)	1296, 1470
SseBI	AGGCCT	1 cut(s)	654
SsiI	CCGC	5 cut(s)	38, 400, 588, 1317, 1671
SspMI	CTAG	6 cut(s)	638, 1016, 1035, 1124, 1164, 1271
SstI	GAGCTC	1 cut(s)	861
StuI	AGGCCT	1 cut(s)	654
StyD4I	CCNGG	1 cut(s)	754
TaaI	ACNGT	5 cut(s)	361, 488, 1012, 1154, 1180
TaiI	ACGT	1 cut(s)	47
TaqI	TCGA	1 cut(s)	280
TasI	AATT	2 cut(s)	1296, 1470
TauI	GCSGC	2 cut(s)	41, 1319
TfiI	GAWTC	4 cut(s)	285, 1139, 1514, 1718
Tru1I	TTAA	4 cut(s)	510, 1358, 1553, 1634
Tru9I	TTAA	4 cut(s)	510, 1358, 1553, 1634
TscAI	CASTG	5 cut(s)	493, 850, 1017, 1333, 1548
TseFI	GTSAC	6 cut(s)	605, 646, 1174, 1238, 1402, 1711
TseI	GCWGC	9 cut(s)	130, 233, 446, 1039, 1076, 1220, 1339, 1391, 1499
Tsp45I	GTSAC	6 cut(s)	605, 646, 1174, 1238, 1402, 1711
TspDTI	ATGAA	6 cut(s)	217, 229, 538, 1179, 1536, 1591
TspGWI	ACGGA	3 cut(s)	526, 551, 1675
TspRI	CASTG	5 cut(s)	493, 850, 1017, 1333, 1548
Tth111I	GACNNNGTC	1 cut(s)	1244
VpaK11BI	GGWCC	1 cut(s)	1668
XmiI	GTMKAC	1 cut(s)	1110
XspI	CTAG	6 cut(s)	638, 1016, 1035, 1124, 1164, 1271
Zsp2I	ATGCAT	1 cut(s)	380

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.