Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0002097

Physical Location & Seq

Genomic Coordinates Forward (+)

41255602 .. 41262658

Gene Structure 7057 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1287800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1728 bp

ATGGCGGGGCAGTCGAGAAAATGGATGATTCTGGTGGCCACAATATGGATTCAGGCGTTTACAGGGACGAACTTCGATTTCTCATCGTATTCGTCGTCGTTGAAGTTGGTGCTGGGAATCTCGCAGGTGCAGTTGAATTACCTGGCGACGGCGTCTGACCTTGGGAAGGTGTTGGGGTGGTCGTCGGGGCTGGCTCTCATGTATTTTCCTCTGTGGGTGGTGCTGTTCATGTCCGCCTTCATGGGATTTATCGGTTACGGCATGCAGTGGCTCGTCATTCGCCAAATCGTCTCCTTGCCTTATTTCCTGATATTTCTTCTTTGTTTGTTGGCTGGGTGTAGCATCTGTTGGTTCAACACCGTATGCTTCGTTCTTTGCATTCGGAACTTCCCAGCTAATCAACCCTTAGCAATATCACTCACAGTAAGCTTCAATGGCGTAAGTGCAGCCTTATACAACCTTGCAGCCAGTGCAATTGACTCATCGTCTACTTCTTTATTTCTAATTTTGAATGGTGTCATTCCCCTTCTCACCTCTGCCGCTGCATTGATCCCTATTCTCCGCCAACCCAGTCTAGACCCTCTCCCACCTGATGGAGTCCGCCGTGACTCCCTCATCTTTCTCCTTTTGAATATTTTAGCTGTTCTAACTGGTGTTTACCTTCTTCTCTTTGGTTCAACCTCATTTGATACAGCAACAGCTCGTCTCTTCTTAGGCGGTGCCATTTTCCTACTCATTTTCCCTCTGTGTATACCTGGCATTGTCTACGCTAGAGATTGGTTTCGCCGTTCCCTTCATTCTAGCATCCGCCTTGAAGGCTCGGGCTTCGTTCTTATTGATGTTGAAGATCTTGAGCTTCACAAAGAACTCCTTTCGCGTGATAACAGTATGAATTACGGGAATGCATGTGTTGATCACCTCTTCAACAACAATGAAAGACCTCCTTCAACTTTGTCATTTAGACATAAGAGCGGATATCAGAGCACCGCAGGCTGTTGTGGGGCAATTTTGGGCAAGGGTCAGTTGGCAATGCTTGGGGAAGACCACACAGCAGGAGCACTTGTACGAAGGTTCGATTTTTGGCTATACTACGTAGCATATTTTTGTGGAGGTACTATTGGTCTTGTGTACAGCAACAATCTGGGGCAGATAGCTCAATCATTAGGACAGAGCTCCAAGACTACAACACTTGTCACACTTTATTCATCATTTTCATTCTTCGGCCGGTTGCTTTCGGCTGCACCAGACTACATACGTGCGAAGGTTTATTTTGCTAGGACAGGATGGTTGACAATTGCACTTTTGCCAACCCCAGTGGCCTTCTTGTTGCTTGCTTCATCAAGCAGTAGCTTCGCTTTGCACACAGGCACTGCATTAATAGGATTGAGCTCCGGATTCATTTTCGCAGCTGCTGTTTCCATCACATCGGAGTTGTTCGGGCCCAACAGCGTAGGTGTTAACCACAACATGCTTATTACAAACATACCAATTGGTTCGCTCGTCTATGGCCTTCTTGCAGCGATCGTTTATGATTCAAATGTGAGGTCTAACCTAAACATCTTAAGGACTGATTCGATTGTGTGCATGGGGAGGGACTGCTACTTCTTAACCTTTGTGGGGTGGGCTTGCATATCTGTTCTTGGGCTAGGTTCAAGTGTACTGTTATTCTTAAGAACCCGACATGCGTATGATCATTTCGAACATAACCGTTCAACGCAACTCTACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Nodulin-like PUCC

Protein Analysis

576

Amino Acids

62.8

Weight (kDa)

8.62

Isoelectric Point (pI)

39.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nodulin-like	PF06813	7 - 252	1.5e-80	Nodulin-like
NFD4_C	PF23262	353 - 563	1.2e-47	NFD4, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002097)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G19450 AT4G19450 AT5G45275
fragaria_vesca	FvH4_5g24410 FvH4_5g24410 FvH4_5g24410 FvH4_5g24410 FvH4_7g07730 FvH4_7g07730 FvH4_7g07730 FvH4_7g07730 FvH4_7g07730
malus_domestica	MD02G1233200.v1.1 MD06G1022100.v1.1 MD07G1080400.v1.1 MD16G1287800.v1.1
prunus_persica	Prupe.2G104800_v2.0.a1 Prupe.5G016300_v2.0.a1
pyrus_communis	pycom06g01600 pycom07g06400
rosa_chinensis	RchiOBHm_Chr1g0342361 RchiOBHm_Chr7g0214811
rosa_laevigata	RLG00000002719 RLG00000002720 RLG00000029156
rosa_multiflora	Rmu_co8462433.1_g000001 Rmu_sc0002308.1_g000032 Rmu_sc0012155.1_g000010
rosa_roxburghii	Rroxscaffold_3G00245010 Rroxscaffold_4G00312980
rosa_rugosa	Rorug01G0151600.1 Rorug07G0147200 Rorug07G0147300 Rorug07G0147400
rosa_samantha	Rh1AG166000 Rh1BG132500 Rh1CG154600 Rh1DG167400 Rh7AG284200 Rh7BG275100 Rh7CG303600 Rh7DG287500
rosa_wichuraiana	Rw1G013870 Rw7G024250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	115
Acc36I	ACCTGC	1 cut(s)	115
AccB1I	GGYRCC	1 cut(s)	719
AccB7I	CCANNNNNTGG	2 cut(s)	45, 593
AccBSI	CCGCTC	1 cut(s)	972
AccI	GTMKAC	3 cut(s)	488, 751, 765
AccII	CGCG	1 cut(s)	877
AccIII	TCCGGA	1 cut(s)	1391
AciI	CCGC	9 cut(s)	5, 234, 540, 562, 601, 717, 808, 972, 987
AclWI	GGATC	1 cut(s)	544
AcoI	YGGCCR	2 cut(s)	36, 1222
AcyI	GRCGYC	1 cut(s)	152
AfaI	GTAC	4 cut(s)	1065, 1114, 1130, 1659
AfiI	CCNNNNNNNGG	5 cut(s)	45, 148, 166, 593, 1617
AflII	CTTAAG	2 cut(s)	1561, 1669
AhdI	GACNNNNNGTC	1 cut(s)	484
AjnI	CCWGG	2 cut(s)	141, 754
Alw21I	GWGCWC	4 cut(s)	986, 1060, 1175, 1391
Alw26I	GTCTC	2 cut(s)	295, 710
AlwI	GGATC	1 cut(s)	544
AlwNI	CAGNNNCTG	2 cut(s)	1370, 1412
Ama87I	CYCGRG	1 cut(s)	820
Aor13HI	TCCGGA	1 cut(s)	1391
AoxI	GGCC	5 cut(s)	36, 1222, 1317, 1439, 1507
ApaI	GGGCCC	1 cut(s)	1443
ApeKI	GCWGC	7 cut(s)	446, 464, 542, 1238, 1406, 1409, 1517
ArsI	GACNNNNNNTTYG	2 cut(s)	1529, 1561
AseI	ATTAAT	1 cut(s)	1376
AspS9I	GGNCC	2 cut(s)	1439, 1440
AsuHPI	GGTGA	2 cut(s)	523, 908
AsuII	TTCGAA	1 cut(s)	1698
AvaI	CYCGRG	1 cut(s)	820
BaeGI	GKGCMC	1 cut(s)	1443
BalI	TGGCCA	1 cut(s)	38
BanI	GGYRCC	1 cut(s)	719
BanII	GRGCYC	3 cut(s)	1175, 1391, 1443
BbsI	GAAGAC	1 cut(s)	1047
Bbv12I	GWGCWC	4 cut(s)	986, 1060, 1175, 1391
BbvI	GCAGC	7 cut(s)	458, 476, 529, 1225, 1396, 1418, 1529
BccI	CCATC	3 cut(s)	587, 1278, 1427
BceAI	ACGGC	4 cut(s)	165, 274, 588, 771
BcgI	CGANNNNNNTGC	2 cut(s)	1333, 1367
BciT130I	CCWGG	2 cut(s)	143, 756
BclI	TGATCA	2 cut(s)	913, 1690
BcoDI	GTCTC	2 cut(s)	295, 710
BfaI	CTAG	5 cut(s)	575, 771, 801, 1275, 1646
BfrI	CTTAAG	2 cut(s)	1561, 1669
BfuAI	ACCTGC	1 cut(s)	115
BglI	GCCNNNNNGGC	1 cut(s)	816
BglII	AGATCT	1 cut(s)	847
BisI	GCNGC	8 cut(s)	447, 465, 540, 543, 1239, 1407, 1410, 1518
BlsI	GCNGC	8 cut(s)	448, 466, 541, 544, 1240, 1408, 1411, 1519
Bme1390I	CCNGG	2 cut(s)	143, 756
BmeRI	GACNNNNNGTC	1 cut(s)	484
BmeT110I	CYCGRG	1 cut(s)	820
BmgT120I	GGNCC	2 cut(s)	1439, 1440
BmiI	GGNNCC	2 cut(s)	721, 1441
BmrFI	CCNGG	2 cut(s)	143, 756
BmrI	ACTGGG	2 cut(s)	564, 1307
BmsI	GCATC	2 cut(s)	351, 813
BmuI	ACTGGG	2 cut(s)	564, 1307
BpiI	GAAGAC	1 cut(s)	1047
Bpu10I	CCTNAGC	1 cut(s)	406
Bpu14I	TTCGAA	1 cut(s)	1698
BpuEI	CTTGAG	1 cut(s)	872
BsaAI	YACGTR	2 cut(s)	1093, 1256
BsaHI	GRCGYC	1 cut(s)	152
BsaJI	CCNNGG	1 cut(s)	160
BsaWI	WCCGGW	1 cut(s)	1391
BsaXI	ACNNNNNCTCC	2 cut(s)	1047, 1077
Bsc4I	CCNNNNNNNGG	5 cut(s)	45, 148, 166, 593, 1617
Bse118I	RCCGGY	1 cut(s)	1224
Bse1I	ACTGG	4 cut(s)	468, 570, 655, 1313
Bse3DI	GCAATG	1 cut(s)	1035
BseAI	TCCGGA	1 cut(s)	1391
BseBI	CCWGG	2 cut(s)	143, 756
BseDI	CCNNGG	1 cut(s)	160
BseGI	GGATG	3 cut(s)	30, 804, 1289
BseLI	CCNNNNNNNGG	5 cut(s)	45, 148, 166, 593, 1617
BseMI	GCAATG	1 cut(s)	1035
BseNI	ACTGG	4 cut(s)	468, 570, 655, 1313
BseSI	GKGCMC	1 cut(s)	1443
BseX3I	CGGCCG	1 cut(s)	1222
BseXI	GCAGC	7 cut(s)	458, 476, 529, 1225, 1396, 1418, 1529
BseYI	CCCAGC	3 cut(s)	112, 332, 391
BsgI	GTGCAG	3 cut(s)	149, 465, 1224
Bsh1236I	CGCG	1 cut(s)	877
Bsh1285I	CGRYCG	2 cut(s)	1225, 1524
BshFI	GGCC	5 cut(s)	38, 1224, 1319, 1441, 1509
BshNI	GGYRCC	1 cut(s)	719
BsiEI	CGRYCG	2 cut(s)	1225, 1524
BsiHKAI	GWGCWC	4 cut(s)	986, 1060, 1175, 1391
BsiHKCI	CYCGRG	1 cut(s)	820
BsiSI	CCGG	2 cut(s)	1225, 1392
BslFI	GGGAC	2 cut(s)	79, 1607
BslI	CCNNNNNNNGG	5 cut(s)	45, 148, 166, 593, 1617
BsmAI	GTCTC	2 cut(s)	295, 710
BsmBI	CGTCTC	2 cut(s)	295, 710
BsmFI	GGGAC	2 cut(s)	79, 1607
BsmI	GAATGC	2 cut(s)	378, 907
BsnI	GGCC	5 cut(s)	38, 1224, 1319, 1441, 1509
BsoBI	CYCGRG	1 cut(s)	820
Bsp119I	TTCGAA	1 cut(s)	1698
Bsp120I	GGGCCC	1 cut(s)	1439
Bsp1286I	GDGCHC	5 cut(s)	986, 1060, 1175, 1391, 1443
Bsp13I	TCCGGA	1 cut(s)	1391
Bsp1407I	TGTACA	1 cut(s)	1128
Bsp143I	GATC	5 cut(s)	549, 847, 913, 1521, 1690
BspACI	CCGC	9 cut(s)	5, 234, 540, 562, 601, 717, 808, 972, 987
BspANI	GGCC	5 cut(s)	38, 1224, 1319, 1441, 1509
BspEI	TCCGGA	1 cut(s)	1391
BspFNI	CGCG	1 cut(s)	877
BspLI	GGNNCC	2 cut(s)	721, 1441
BspMI	ACCTGC	1 cut(s)	115
BspPI	GGATC	1 cut(s)	544
BspT104I	TTCGAA	1 cut(s)	1698
BspT107I	GGYRCC	1 cut(s)	719
BspTI	CTTAAG	2 cut(s)	1561, 1669
BsrBI	CCGCTC	1 cut(s)	972
BsrDI	GCAATG	1 cut(s)	1035
BsrFI	RCCGGY	1 cut(s)	1224
BsrGI	TGTACA	1 cut(s)	1128
BsrI	ACTGG	4 cut(s)	468, 570, 655, 1313
BssAI	RCCGGY	1 cut(s)	1224
BssECI	CCNNGG	1 cut(s)	160
BssMI	GATC	5 cut(s)	549, 847, 913, 1521, 1690
BssNAI	GTATAC	1 cut(s)	752
BssNI	GRCGYC	1 cut(s)	152
BssT1I	CCWWGG	1 cut(s)	160
Bst1107I	GTATAC	1 cut(s)	752
Bst2UI	CCWGG	2 cut(s)	143, 756
Bst4CI	ACNGT	5 cut(s)	361, 424, 887, 1662, 1709
Bst6I	CTCTTC	2 cut(s)	713, 926
BstACI	GRCGYC	1 cut(s)	152
BstAFI	CTTAAG	2 cut(s)	1561, 1669
BstAPI	GCANNNNNTGC	1 cut(s)	470
BstAUI	TGTACA	1 cut(s)	1128
BstBAI	YACGTR	2 cut(s)	1093, 1256
BstBI	TTCGAA	1 cut(s)	1698
BstC8I	GCNNGC	5 cut(s)	192, 263, 991, 1332, 1627
BstDEI	CTNAG	2 cut(s)	406, 712
BstENI	CCTNNNNNAGG	1 cut(s)	164
BstF5I	GGATG	3 cut(s)	30, 804, 1289
BstFNI	CGCG	1 cut(s)	877
BstKTI	GATC	5 cut(s)	552, 850, 916, 1524, 1693
BstMAI	GTCTC	2 cut(s)	295, 710
BstMBI	GATC	5 cut(s)	549, 847, 913, 1521, 1690
BstMCI	CGRYCG	2 cut(s)	1225, 1524
BstMWI	GCNNNNNNNGC	4 cut(s)	435, 470, 816, 990
BstNI	CCWGG	2 cut(s)	143, 756
BstNSI	RCATGY	4 cut(s)	265, 909, 1471, 1685
BstSCI	CCNGG	2 cut(s)	141, 754
BstSLI	GKGCMC	1 cut(s)	1443
BstSNI	TACGTA	1 cut(s)	1093
BstUI	CGCG	1 cut(s)	877
BstV1I	GCAGC	7 cut(s)	458, 476, 529, 1225, 1396, 1418, 1529
BstV2I	GAAGAC	1 cut(s)	1047
BstX2I	RGATCY	1 cut(s)	847
BstYI	RGATCY	1 cut(s)	847
BstZ17I	GTATAC	1 cut(s)	752
BstZI	CGGCCG	1 cut(s)	1222
BsuRI	GGCC	5 cut(s)	38, 1224, 1319, 1441, 1509
BtsCI	GGATG	3 cut(s)	30, 804, 1289
BtsI	GCAGTG	2 cut(s)	272, 1368
BtsIMutI	CAGTG	4 cut(s)	272, 475, 1320, 1368
BveI	ACCTGC	1 cut(s)	115
Cac8I	GCNNGC	5 cut(s)	192, 263, 991, 1332, 1627
CaiI	CAGNNNCTG	2 cut(s)	1370, 1412
Cfr10I	RCCGGY	1 cut(s)	1224
Cfr13I	GGNCC	2 cut(s)	1439, 1440
CseI	GACGC	1 cut(s)	141
Csp6I	GTAC	4 cut(s)	1064, 1113, 1129, 1658
CspCI	CAANNNNNGTGG	2 cut(s)	1296, 1331
CviAII	CATG	8 cut(s)	199, 229, 241, 262, 906, 1468, 1585, 1682
CviQI	GTAC	4 cut(s)	1064, 1113, 1129, 1658
DdeI	CTNAG	2 cut(s)	406, 712
DpnI	GATC	5 cut(s)	551, 849, 915, 1523, 1692
DpnII	GATC	5 cut(s)	549, 847, 913, 1521, 1690
DriI	GACNNNNNGTC	1 cut(s)	484
EaeI	YGGCCR	2 cut(s)	36, 1222
EagI	CGGCCG	1 cut(s)	1222
Eam1104I	CTCTTC	2 cut(s)	713, 926
Eam1105I	GACNNNNNGTC	1 cut(s)	484
EarI	CTCTTC	2 cut(s)	713, 926
EciI	GGCGGA	4 cut(s)	223, 551, 590, 797
Ecl136II	GAGCTC	2 cut(s)	1173, 1389
EclXI	CGGCCG	1 cut(s)	1222
Eco105I	TACGTA	1 cut(s)	1093
Eco130I	CCWWGG	1 cut(s)	160
Eco24I	GRGCYC	3 cut(s)	1175, 1391, 1443
Eco32I	GATATC	1 cut(s)	977
Eco52I	CGGCCG	1 cut(s)	1222
Eco53kI	GAGCTC	2 cut(s)	1173, 1389
Eco88I	CYCGRG	1 cut(s)	820
EcoICRI	GAGCTC	2 cut(s)	1173, 1389
EcoNI	CCTNNNNNAGG	1 cut(s)	164
EcoRII	CCWGG	2 cut(s)	141, 754
EcoRV	GATATC	1 cut(s)	977
EcoT14I	CCWWGG	1 cut(s)	160
EcoT22I	ATGCAT	1 cut(s)	907
EcoT38I	GRGCYC	3 cut(s)	1175, 1391, 1443
ErhI	CCWWGG	1 cut(s)	160
Esp3I	CGTCTC	2 cut(s)	295, 710
FaeI	CATG	8 cut(s)	202, 232, 244, 265, 909, 1471, 1588, 1685
FalI	AAGNNNNNCTT	4 cut(s)	855, 887, 928, 960
FaqI	GGGAC	2 cut(s)	79, 1607
FatI	CATG	8 cut(s)	198, 228, 240, 261, 905, 1467, 1584, 1681
FbaI	TGATCA	2 cut(s)	913, 1690
FblI	GTMKAC	3 cut(s)	488, 751, 765
Fnu4HI	GCNGC	8 cut(s)	447, 465, 540, 543, 1239, 1407, 1410, 1518
FokI	GGATG	3 cut(s)	37, 791, 1296
FriOI	GRGCYC	3 cut(s)	1175, 1391, 1443
Fsp4HI	GCNGC	8 cut(s)	447, 465, 540, 543, 1239, 1407, 1410, 1518
FspBI	CTAG	5 cut(s)	575, 771, 801, 1275, 1646
GluI	GCNGC	8 cut(s)	447, 465, 540, 543, 1239, 1407, 1410, 1518
GsaI	CCCAGC	3 cut(s)	116, 336, 395
HaeIII	GGCC	5 cut(s)	38, 1224, 1319, 1441, 1509
HapII	CCGG	2 cut(s)	1225, 1392
HgaI	GACGC	1 cut(s)	141
Hin1I	GRCGYC	1 cut(s)	152
Hin1II	CATG	8 cut(s)	202, 232, 244, 265, 909, 1471, 1588, 1685
HincII	GTYRAC	2 cut(s)	1290, 1459
HindII	GTYRAC	2 cut(s)	1290, 1459
HindIII	AAGCTT	1 cut(s)	427
HinfI	GANTC	9 cut(s)	28, 49, 117, 479, 597, 608, 1395, 1532, 1571
HpaI	GTTAAC	1 cut(s)	1459
HpaII	CCGG	2 cut(s)	1225, 1392
HphI	GGTGA	2 cut(s)	523, 908
Hpy166II	GTNNAC	9 cut(s)	60, 489, 658, 752, 766, 1129, 1290, 1459, 1658
Hpy188I	TCNGA	4 cut(s)	157, 384, 981, 1429
Hpy188III	TCNNGA	5 cut(s)	15, 307, 575, 851, 1392
Hpy8I	GTNNAC	9 cut(s)	60, 489, 658, 752, 766, 1129, 1290, 1459, 1658
Hpy99I	CGWCG	4 cut(s)	97, 100, 151, 187
HpyCH4III	ACNGT	5 cut(s)	361, 424, 887, 1662, 1709
HpyCH4IV	ACGT	2 cut(s)	1092, 1255
HpyF10VI	GCNNNNNNNGC	4 cut(s)	435, 470, 816, 990
HpyF3I	CTNAG	2 cut(s)	406, 712
HpySE526I	ACGT	2 cut(s)	1092, 1255
Hsp92I	GRCGYC	1 cut(s)	152
Hsp92II	CATG	8 cut(s)	202, 232, 244, 265, 909, 1471, 1588, 1685
Kpn2I	TCCGGA	1 cut(s)	1391
Ksp22I	TGATCA	2 cut(s)	913, 1690
KspAI	GTTAAC	1 cut(s)	1459
Kzo9I	GATC	5 cut(s)	549, 847, 913, 1521, 1690
LmnI	GCTCC	3 cut(s)	1055, 1178, 1394
Lsp1109I	GCAGC	7 cut(s)	458, 476, 529, 1225, 1396, 1418, 1529
LweI	GCATC	2 cut(s)	351, 813
MaeI	CTAG	5 cut(s)	575, 771, 801, 1275, 1646
MaeII	ACGT	2 cut(s)	1092, 1255
MaeIII	GTNAC	3 cut(s)	254, 605, 1192
MalI	GATC	5 cut(s)	551, 849, 915, 1523, 1692
MbiI	CCGCTC	1 cut(s)	972
MboI	GATC	5 cut(s)	549, 847, 913, 1521, 1690
MboII	GAAGA	7 cut(s)	308, 656, 700, 857, 913, 1052, 1210
MfeI	CAATTG	3 cut(s)	474, 1293, 1488
MflI	RGATCY	1 cut(s)	847
MhlI	GDGCHC	5 cut(s)	986, 1060, 1175, 1391, 1443
MlsI	TGGCCA	1 cut(s)	38
MluCI	AATT	7 cut(s)	136, 474, 504, 892, 1005, 1293, 1488
MluNI	TGGCCA	1 cut(s)	38
MlyI	GAGTC	3 cut(s)	473, 602, 606
Mox20I	TGGCCA	1 cut(s)	38
Mph1103I	ATGCAT	1 cut(s)	907
MroI	TCCGGA	1 cut(s)	1391
MscI	TGGCCA	1 cut(s)	38
MseI	TTAA	5 cut(s)	1376, 1458, 1562, 1607, 1670
MslI	CAYNNNNRTG	1 cut(s)	1686
Msp20I	TGGCCA	1 cut(s)	38
MspA1I	CMGCKG	2 cut(s)	542, 1409
MspCI	CTTAAG	2 cut(s)	1561, 1669
MspI	CCGG	2 cut(s)	1225, 1392
MspR9I	CCNGG	2 cut(s)	143, 756
MunI	CAATTG	3 cut(s)	474, 1293, 1488
Mva1269I	GAATGC	2 cut(s)	378, 907
MvaI	CCWGG	2 cut(s)	143, 756
MvnI	CGCG	1 cut(s)	877
MwoI	GCNNNNNNNGC	4 cut(s)	435, 470, 816, 990
NdeII	GATC	5 cut(s)	549, 847, 913, 1521, 1690
NlaIII	CATG	8 cut(s)	202, 232, 244, 265, 909, 1471, 1588, 1685
NlaIV	GGNNCC	2 cut(s)	721, 1441
NmuCI	GTSAC	2 cut(s)	605, 1192
NsiI	ATGCAT	1 cut(s)	907
NspI	RCATGY	4 cut(s)	265, 909, 1471, 1685
NspV	TTCGAA	1 cut(s)	1698
PaeI	GCATGC	1 cut(s)	265
PaqCI	CACCTGC	1 cut(s)	115
PcsI	WCGNNNNNNNCGW	1 cut(s)	92
PctI	GAATGC	2 cut(s)	378, 907
PfeI	GAWTC	6 cut(s)	28, 49, 117, 1395, 1532, 1571
PflFI	GACNNNGTC	1 cut(s)	151
PflMI	CCANNNNNTGG	2 cut(s)	45, 593
PkrI	GCNGC	8 cut(s)	448, 466, 541, 544, 1240, 1408, 1411, 1519
Ple19I	CGATCG	1 cut(s)	1524
PleI	GAGTC	3 cut(s)	473, 602, 605
PpsI	GAGTC	3 cut(s)	473, 602, 605
Ppu21I	YACGTR	2 cut(s)	1093, 1256
PshBI	ATTAAT	1 cut(s)	1376
Psp124BI	GAGCTC	2 cut(s)	1175, 1391
Psp6I	CCWGG	2 cut(s)	141, 754
PspFI	CCCAGC	3 cut(s)	112, 332, 391
PspGI	CCWGG	2 cut(s)	141, 754
PspN4I	GGNNCC	2 cut(s)	721, 1441
PspOMI	GGGCCC	1 cut(s)	1439
PspPI	GGNCC	2 cut(s)	1439, 1440
PsrI	GAACNNNNNNTAC	2 cut(s)	354, 386
PstNI	CAGNNNCTG	2 cut(s)	1370, 1412
PsuI	RGATCY	1 cut(s)	847
PsyI	GACNNNGTC	1 cut(s)	151
PvuI	CGATCG	1 cut(s)	1524
PvuII	CAGCTG	1 cut(s)	1409
RsaI	GTAC	4 cut(s)	1065, 1114, 1130, 1659
RsaNI	GTAC	4 cut(s)	1064, 1113, 1129, 1658
RseI	CAYNNNNRTG	1 cut(s)	1686
SacI	GAGCTC	2 cut(s)	1175, 1391
SaqAI	TTAA	5 cut(s)	1376, 1458, 1562, 1607, 1670
SatI	GCNGC	8 cut(s)	447, 465, 540, 543, 1239, 1407, 1410, 1518
Sau3AI	GATC	5 cut(s)	549, 847, 913, 1521, 1690
Sau96I	GGNCC	2 cut(s)	1439, 1440
SchI	GAGTC	3 cut(s)	473, 602, 606
ScrFI	CCNGG	2 cut(s)	143, 756
SduI	GDGCHC	5 cut(s)	986, 1060, 1175, 1391, 1443
SfaNI	GCATC	2 cut(s)	351, 813
SfuI	TTCGAA	1 cut(s)	1698
SmiMI	CAYNNNNRTG	1 cut(s)	1686
SmlI	CTYRAG	3 cut(s)	851, 1561, 1669
SmoI	CTYRAG	3 cut(s)	851, 1561, 1669
SnaBI	TACGTA	1 cut(s)	1093
SphI	GCATGC	1 cut(s)	265
Sse9I	AATT	7 cut(s)	136, 474, 504, 892, 1005, 1293, 1488
SsiI	CCGC	9 cut(s)	5, 234, 540, 562, 601, 717, 808, 972, 987
SspI	AATATT	1 cut(s)	634
SspMI	CTAG	5 cut(s)	575, 771, 801, 1275, 1646
SstI	GAGCTC	2 cut(s)	1175, 1391
StyD4I	CCNGG	2 cut(s)	141, 754
StyI	CCWWGG	1 cut(s)	160
TaaI	ACNGT	5 cut(s)	361, 424, 887, 1662, 1709
TaiI	ACGT	2 cut(s)	1095, 1258
TaqI	TCGA	5 cut(s)	14, 75, 1074, 1574, 1698
TasI	AATT	7 cut(s)	136, 474, 504, 892, 1005, 1293, 1488
TatI	WGTACW	2 cut(s)	1128, 1657
TauI	GCSGC	1 cut(s)	542
TfiI	GAWTC	6 cut(s)	28, 49, 117, 1395, 1532, 1571
Tru1I	TTAA	5 cut(s)	1376, 1458, 1562, 1607, 1670
Tru9I	TTAA	5 cut(s)	1376, 1458, 1562, 1607, 1670
TscAI	CASTG	4 cut(s)	272, 475, 1320, 1375
TseFI	GTSAC	2 cut(s)	605, 1192
TseI	GCWGC	7 cut(s)	446, 464, 542, 1238, 1406, 1409, 1517
Tsp45I	GTSAC	2 cut(s)	605, 1192
TspDTI	ATGAA	9 cut(s)	217, 229, 785, 905, 948, 1194, 1203, 1326, 1387
TspRI	CASTG	4 cut(s)	272, 475, 1320, 1375
Tth111I	GACNNNGTC	1 cut(s)	151
Van91I	CCANNNNNTGG	2 cut(s)	45, 593
Vha464I	CTTAAG	2 cut(s)	1561, 1669
VspI	ATTAAT	1 cut(s)	1376
XagI	CCTNNNNNAGG	1 cut(s)	164
XbaI	TCTAGA	1 cut(s)	574
XceI	RCATGY	4 cut(s)	265, 909, 1471, 1685
XmiI	GTMKAC	3 cut(s)	488, 751, 765
XspI	CTAG	5 cut(s)	575, 771, 801, 1275, 1646
Zsp2I	ATGCAT	1 cut(s)	907

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.