Rorug07G0147400

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0002097
Physical Location & Seq
Forward (+)
11668410 .. 11674745
6336 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0147400.1

Sequence Viewer

Length: 4596 bp
ATGGATAGGTGGGAAGGTCGTAAGCGAAAGAAGATGACAATTGGTGGTTACATGGGCATGTCACACATTAAAAGCACTATAAGGAGCAAGACAAGTTCTCCCGGACTTTCCAGATCACTGGGGAACAACATGGGGGAGGGTTTCTGGACCTTATTCTGCACCAGTTCCGAGTGTTCAAATGGGTCTGGAAATGAATGCAGTTCTGGCATTCTAGCCATTATCAATCCAAATTCATGTATCAACAATATTGTCGTCATTGCAGTTGATATTTTGCTCCTACTCATCTTGTTATGTTTCCTTATGTATAGAATAGCATCAAAGAAGATTATAGCACCATCACAGCCTCAAACCTTCTCTCCAGTATCAGTTGTGTCAATCAGTTTCAATGCAGGTTTGGCTTTGGCCTACTTGGGGTCTGGGATATGGACAATCATTGAGAAAGTATATACATATCACACGGTTTTACCTCTACATGGATGGCTAGTATATTTTTTTCAAGGGTTTACATGGTTGGGCCTGGGTTTTACCATAAACCTCTCAAAACCACACCTTCCGCATAACGCAATAATGAAATTCTTTTCAATTTTTGGTTTCTTAATTGCAGTTTTCCTTTGTGGTTCATCAATTTGGGAATCTATTCTGGATGGGGCCTTATCAGTTCTGATTGTTTTAAACATTCTTTGTTTCCCTGGATCAATTATCTTGCTCTTTAGTGCATTTGAAGGAACAAATAATGCAAAAGGTGATCCAGAAGTCCATAATGGTCATGCTTTCTACACACCTTTACCAGGTGCAGAACCAGATATCACAGGTGATATCAGTCCAAATGACACTGTAACTCCTTTTGCAAAAGCTGGATTGTTTAGTAAAATGTCATTTTGGTGGTTGAATCCATTAATGAAGAAAGGTAAGGAAAAGATCCTTGAGAATGAAGATATTCCACAATTGCGAGAAGCTGATCGAGCACGAACATTGCACTCAATATTCAGGGAGCAATTGCATAAAAGAAAGGAAAGACATCCGTCTAGTGACCCTTCTATTCTGACCATAATTTTTTTCTGCCAGTGGAAAGCAATTTTGGTTTCAGGGTTCTTTGCACTCCTCAAGATTCTCGCAATGACTGCCTCCCCACTGTTTCTCATAGCTTTCATCAAGGTTGTTGATGGCGATGCAGCTTTTAAATATGAGGTTTATGCACTGACTGCTGGTCTGTTCGTTGTGAAAATCGTGGAGTCACTGTCAGAGAGGCAATGGTTCTTTAGAACTAGACTTATTGGACTCCAGGTAAGATCATTGATATCTGCAGCAGTTTATCAGAAGCAACTAAGACTCTCAAATGCTGCCAAGACGACTCATTCTCCTGGTGAGATAGTGAACTATGTAACAGTGGATGCTTACAGAATCGGTGAATTTCCTTACTGGTTTCATCAGATATGGTCCACAAACCTTCAATTGTGTCTCTCATTACTTATTATCTACTTCGCTGTTGGGGCAGCAACTCTTGCAGCTCTAACTGTTCTCATATTGACTATGGTGGTAAGCTCTCCATTGGCGAAGTTGCAGCATGAGTACCAAACAAAGTTCATGATTGCACAAAACAAGAGGTTGAGGGCCATTACAGAAGCACTATCAAATATGAAAATCTTGAAGCTGTATTCTTGGGAGACAAATTTCAAGAAAGTCATAGAAGGTTTTAGAGCAGAAGAACTAAAATGGATAGCTAAAGTACTGTCACAAAAGGGATATTGTAATGCTTTATTCTGGACAGCTCCTGTTTTGGCAGCAGCTGTCACCTTTTGGACATGTTACTTCCTGGGATTTACACTCACAGCTAGTAATGTTTTCACGTTTCTAGCAACTTTGCGTATACTTCAGGAGCCAATTAGGCTAATCCCAGACGTCTTTGGAGCATTTATTGAAGCAAAGGTTTCATTTTCTCGGATTGTGAAGTTCCTTGATGCCCCAGAGTTGGAGAACAGACATACAAGGAAACAGAGCTGTGACAAGGAGGTTGAGCATTCAATTTTCGTTAGAGCCTCTGAAATTTCATGGGATATAGAAGCTACAAGGGCCACATTGAGGGACATTAATTTAGTGGTTAAGCAAGGAGAAAAAGTAGCTATTTGTGGAGAGGTTGGCTCAGGAAAATCAACCCTTTTAGCTGCAATTCTTGGAGAGGTTCCACGCATCAATGGCATTGTTCAAGTATATGGCACGATAGCATATGTCTCTCAGTCTGCATGGATCCAAACAGGAACTATACAAGATAATATTCTGTTTGGGACTCCCATGGATCATGTTAGATACCAAGAAACTCTTGAGAAGTGTTCACTCATAAAGGACCTCAAGATTCTTCCATTCGGCGATCTCACTCAGATAGGAGAAAGAGGTGTCAATCTAAGTGGTGGCCAGAAACAGCGCATTCAACTTGCACGTGCATTGTATCAAAATGCTGAAGTCTACTTATTGGATGATCCTTTCAGTGCTGTTGATGCTCATACTGCCATAAGCCTGTTCAATGAATATGTCATGGGAGCTCTGGCAGAGAAGACAGTGTTGCTTGTTACACACCAAGTCGACTTCCTTCCAGCTTTTAATTCAATATTGTTGATGTCTTGGGGAAAAATTTCAAGAGCAGCTCCTTACGACGAGTTACTGGCTTCTTGTCAAGAGTTCCAAGACCTTGTCAATGCACATAATGACACTACTGGTTCCGGGAGGCAAGTTGAATATGCTTCTACTAGGAAACAGAAATTGGCTGCAGAGGAGATTGAGAAAGAAAGAACAGAGGTAGAGCTAAAAGAATCTTCAGGAGATCAATTGATTAAGAAAGAAGAGAGAGAAACAGGAGACACAGGGTTCAAGCCATACATTCAGTATTTGAAGCATAGCAGGGGCTTTTTGCATTTCTCCTTATCAGTTCTTTTCTTGTTTCTGTTCATTGTTGGGCAATTAATTCAGAATTATTGGTTGGCTTTGAATCTTCAGGATTATAGCGTGTCCAGAGTCAAACTCTTCACAGTTTATTCTGTGATCTTCTGTATAATGTCATTCTTTTTGCTTCTTCGATCTTTTATGATAGTTGATTTAGGATGTGGAGCTTCAGAGTCTATTTTCTCTGCACTACTGAACTCTCTTTGCCGAGCACCAATGCTGTTTTATGATTCAACACCTGTGGGGAGGATACTTAGTCGGGTATCTACTGATATGAATATCATCGACCTTGAAGTAGGTTTAAAGTTGGCATTTGCTGCTGGGGCGATTGTTAGCGCCTACAGCATATTTTTGGTATTGGTTGTTCTTGCTTGGCCACTCGTGTTTCTGATTGCACCTACAATTTATCTTACTTATCTGTTTCAGAATTACTACTTTGCTTCTGCAAAAGAGTTAATGCGAATGAATGGCACGACTAAGTCTGCGATTGCAAGCCAACTTTCTGAATCTATTGCTGGAGCCATGACAATCAGAGCTTTTGGAGAGGAGAAACGATTCTACTCAAAAAACTTGGAATTTATTGATGCAAATTCTAGTGCTGACTTCAATAGTTTCTCAACAAATGAGTGGTTAACCGAACGTCTGGAATTTCTGTGTGCTATTGTTCTCTCAGCCTCAGCCATTGCCATTACTTGGATAGATGCTTCTTCCTCTGGATTTGTTGGGATGACACTATCATATGGTCTCTCATTAAATGTATTCCTCGTAACTTCTGTCCAACTTCAGTGCATGATAGGAAATTCAATTGTTTCTGTTGAAAGGGTAGAACAATACATGCATATTACCAGTGAATCTGCAGAAGTAATAGAAGAAAACCGACCTGCACACAATTGGCCTGTTGTTGGTAAAGTGGAAATATGTGACTTAAAGGTCAGATATAGGCCAAATTCTCCTTTGGTTCTTCGTGGGATAAATTGCATAATTGAAGGAGGGTACAAAGTTGGAATCGTTGGCCGGACTGGAAGTGGAAAGACAACTCTTATCAGCGTTTTGTTTCGTCTGGTAGAGCCTACAGAGGGAAAAGTCATTATAGATGATTATGACATTTGTACCATTGGTCTTCATGATTTAAGATCACGTTTGGGGATCATCCCACAAGAGCCAACCTTATTCGGTGGCTCTGTGAGATTCAACCTTGACCCCTTATCAGAGCATACTGATCTTGAAATATGGGAGGTTCTTGAGAAATGCCAGTTACGAAAGGCCATTGAAGAGAAAGAAGAGGGTCTGGACACCTTTGTTGTGCAAGATGGAACAAACTGGAGCACGGGGCAGCGTCAACTATTCTGTTTGGGACGTGCTTTACTGAAGAGGAGCCGAATACTAGTGCTGGATGAAGCCACTGCATCAATGGACAATGCAACAGACTCTATTCTCCAGAAAACTATAAGAAAGGAATTTGCAGACTGCACTGTAATAACAGTGGCCCATAGGATACCAACCGTGATGGACTGCACTAAAGTTCTTGCTATAAGTGATGGGCTATTAGTAGAGTATGATGAGCCAACGAAGCTGATGAACAATGAGGGGTCACTGTTTGGGCAGCTTGTGAAGGAATACTGGTCGCGTTCTGCCAATGCTGGCATCCATTCAGAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nodulin-like PUCC

Protein Analysis

1531

Amino Acids

171.02

Weight (kDa)

6.74

Isoelectric Point (pI)

36.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABCC10_N PF24358 46 - 244 6.7e-69 ABCC10 N-terminal domain
ABC_membrane PF00664 360 - 627 6e-27 ABC transporter transmembrane region
ABC_tran PF00005 693 - 827 1.9e-21 ABC transporter
ABC_membrane PF00664 972 - 1224 5.5e-26 ABC transporter transmembrane region
ABC_tran PF00005 1299 - 1447 1.2e-23 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 3863
AatII GACGTC 1 cut(s) 1902
Acc36I ACCTGC 2 cut(s) 380, 3823
AccB7I CCANNNNNTGG 1 cut(s) 3627
AccI GTMKAC 3 cut(s) 1867, 2460, 2577
AccII CGCG 1 cut(s) 4561
AciI CCGC 1 cut(s) 554
AclWI GGATC 8 cut(s) 700, 740, 913, 2239, 2252, 2301, 2468, 4088
AcoI YGGCCR 3 cut(s) 2407, 3308, 3946
AcuI CTGAAG 7 cut(s) 1856, 2475, 2793, 2969, 3087, 3701, 4322
AcvI CACGTG 1 cut(s) 2435
AcyI GRCGYC 1 cut(s) 1899
AfaI GTAC 4 cut(s) 1571, 1728, 3929, 4045
AfiI CCNNNNNNNGG 8 cut(s) 411, 473, 788, 1969, 2079, 3627, 3836, 4010
AflIII ACRYGT 1 cut(s) 1802
AhdI GACNNNNNGTC 1 cut(s) 1206
AhlI ACTAGT 1 cut(s) 4318
AjiI CACGTC 1 cut(s) 4292
AjnI CCWGG 6 cut(s) 516, 688, 787, 1281, 1360, 1812
AjuI GAANNNNNNNTTGG 6 cut(s) 1061, 1093, 2738, 2770, 2954, 2986
Alw21I GWGCWC 4 cut(s) 967, 2539, 3148, 4262
Alw26I GTCTC 5 cut(s) 1463, 1658, 2233, 2844, 3683
AlwI GGATC 8 cut(s) 700, 740, 913, 2239, 2252, 2301, 2468, 4088
AlwNI CAGNNNCTG 2 cut(s) 1772, 1787
AseI ATTAAT 3 cut(s) 896, 2088, 2954
Asp700I GAANNNNTTC 4 cut(s) 636, 936, 2626, 3488
AspLEI GCGC 2 cut(s) 2421, 3272
AspS9I GGNCC 8 cut(s) 147, 514, 648, 1437, 1611, 2070, 2341, 4420
AsuC2I CCSGG 2 cut(s) 102, 2716
AsuHPI GGTGA 5 cut(s) 755, 824, 1376, 1418, 1783
AvaII GGWCC 3 cut(s) 147, 1437, 2341
BaeI ACNNNNGTAYC 2 cut(s) 4027, 4060
BalI TGGCCA 2 cut(s) 2409, 3310
BamHI GGATCC 1 cut(s) 2244
BanII GRGCYC 1 cut(s) 2539
BauI CACGAG 1 cut(s) 3314
BbrPI CACGTG 1 cut(s) 2435
BbsI GAAGAC 2 cut(s) 2555, 4046
Bbv12I GWGCWC 4 cut(s) 967, 2539, 3148, 4262
BbvCI CCTCAGC 1 cut(s) 3610
BccI CCATC 7 cut(s) 343, 471, 638, 1157, 4238, 4435, 4466
BciT130I CCWGG 6 cut(s) 518, 690, 789, 1283, 1362, 1814
BciVI GTATCC 2 cut(s) 3177, 4422
BcnI CCSGG 2 cut(s) 102, 2716
BcoDI GTCTC 5 cut(s) 1463, 1658, 2233, 2844, 3683
BcuI ACTAGT 1 cut(s) 4318
BfaI CTAG 9 cut(s) 212, 482, 1026, 1266, 1833, 1853, 2742, 3528, 4319
BfmI CTRYAG 5 cut(s) 1302, 2760, 3274, 3789, 4005
BfoI RGCGCY 1 cut(s) 3273
BfuAI ACCTGC 2 cut(s) 380, 3823
BfuI GTATCC 2 cut(s) 3177, 4422
BglI GCCNNNNNGGC 1 cut(s) 1885
BmcAI AGTACT 1 cut(s) 1728
Bme1390I CCNGG 8 cut(s) 102, 518, 690, 789, 1283, 1362, 1814, 2716
Bme18I GGWCC 3 cut(s) 147, 1437, 2341
BmeRI GACNNNNNGTC 1 cut(s) 1206
BmgBI CACGTC 1 cut(s) 4292
BmgT120I GGNCC 8 cut(s) 147, 514, 648, 1437, 1611, 2070, 2341, 4420
BmiI GGNNCC 7 cut(s) 649, 1878, 2181, 2246, 2713, 3454, 4310
BmrFI CCNGG 8 cut(s) 102, 518, 690, 789, 1283, 1362, 1814, 2716
BmrI ACTGGG 1 cut(s) 128
BmuI ACTGGG 1 cut(s) 128
BoxI GACNNNNGTC 1 cut(s) 1021
BpiI GAAGAC 2 cut(s) 2555, 4046
BplI GAGNNNNNCTC 4 cut(s) 2123, 2155, 2997, 3029
BpmI CTGGAG 5 cut(s) 342, 1265, 3471, 4276, 4355
Bpu10I CCTNAGC 2 cut(s) 2140, 3610
BpuEI CTTGAG 5 cut(s) 944, 1088, 2330, 2339, 4196
BpuMI CCSGG 2 cut(s) 102, 2716
BsaAI YACGTR 1 cut(s) 2435
BsaHI GRCGYC 1 cut(s) 1899
BsaI GGTCTC 1 cut(s) 3683
BsaJI CCNNGG 4 cut(s) 517, 688, 1813, 2289
Bsc4I CCNNNNNNNGG 8 cut(s) 411, 473, 788, 1969, 2079, 3627, 3836, 4010
Bse3DI GCAATG 5 cut(s) 255, 971, 1122, 1256, 3615
BseBI CCWGG 6 cut(s) 518, 690, 789, 1283, 1362, 1814
BseDI CCNNGG 4 cut(s) 517, 688, 1813, 2289
BseLI CCNNNNNNNGG 8 cut(s) 411, 473, 788, 1969, 2079, 3627, 3836, 4010
BseMI GCAATG 5 cut(s) 255, 971, 1122, 1256, 3615
BseMII CTCAG 5 cut(s) 2154, 2246, 2387, 3618, 3624
BseRI GAGGAG 4 cut(s) 1091, 2780, 3494, 4321
BseYI CCCAGC 1 cut(s) 3254
BsgI GTGCAG 6 cut(s) 142, 813, 3105, 3801, 4387, 4432
Bsh1236I CGCG 1 cut(s) 4561
BsiHKAI GWGCWC 4 cut(s) 967, 2539, 3148, 4262
BsiSI CCGG 3 cut(s) 102, 2715, 3949
BslFI GGGAC 3 cut(s) 2096, 2296, 4302
BslI CCNNNNNNNGG 8 cut(s) 411, 473, 788, 1969, 2079, 3627, 3836, 4010
BsmAI GTCTC 5 cut(s) 1463, 1658, 2233, 2844, 3683
BsmFI GGGAC 3 cut(s) 2096, 2296, 4302
BsmI GAATGC 4 cut(s) 200, 207, 2017, 2421
Bso31I GGTCTC 1 cut(s) 3683
Bsp1286I GDGCHC 4 cut(s) 967, 2539, 3148, 4262
Bsp19I CCATGG 1 cut(s) 2289
BspACI CCGC 1 cut(s) 554
BspCNI CTCAG 5 cut(s) 2153, 2245, 2386, 3617, 3623
BspFNI CGCG 1 cut(s) 4561
BspHI TCATGA 2 cut(s) 1584, 4057
BspLI GGNNCC 7 cut(s) 649, 1878, 2181, 2246, 2713, 3454, 4310
BspMAI CTGCAG 3 cut(s) 1306, 2764, 3793
BspMI ACCTGC 2 cut(s) 380, 3823
BspPI GGATC 8 cut(s) 700, 740, 913, 2239, 2252, 2301, 2468, 4088
BspTNI GGTCTC 1 cut(s) 3683
BsrDI GCAATG 5 cut(s) 255, 971, 1122, 1256, 3615
BssECI CCNNGG 4 cut(s) 517, 688, 1813, 2289
BssNAI GTATAC 1 cut(s) 1868
BssNI GRCGYC 1 cut(s) 1899
BssSI CACGAG 1 cut(s) 3314
BssT1I CCWWGG 1 cut(s) 2289
Bst1107I GTATAC 1 cut(s) 1868
Bst2BI CACGAG 1 cut(s) 3314
Bst2UI CCWGG 6 cut(s) 518, 690, 789, 1283, 1362, 1814
Bst6I CTCTTC 5 cut(s) 2829, 3020, 4200, 4209, 4298
BstACI GRCGYC 1 cut(s) 1899
BstAPI GCANNNNNTGC 4 cut(s) 1121, 1202, 1502, 3251
BstBAI YACGTR 1 cut(s) 2435
BstC8I GCNNGC 2 cut(s) 3427, 4576
BstDEI CTNAG 9 cut(s) 1325, 2140, 2232, 2373, 2399, 3188, 3411, 3604, 3610
BstDSI CCRYGG 1 cut(s) 2289
BstENI CCTNNNNNAGG 1 cut(s) 786
BstFNI CGCG 1 cut(s) 4561
BstH2I RGCGCY 1 cut(s) 3273
BstHHI GCGC 2 cut(s) 2421, 3272
BstMAI GTCTC 5 cut(s) 1463, 1658, 2233, 2844, 3683
BstNI CCWGG 6 cut(s) 518, 690, 789, 1283, 1362, 1814
BstNSI RCATGY 3 cut(s) 61, 1806, 3772
BstPAI GACNNNNGTC 1 cut(s) 1021
BstSCI CCNGG 8 cut(s) 100, 516, 688, 787, 1281, 1360, 1812, 2714
BstSFI CTRYAG 5 cut(s) 1302, 2760, 3274, 3789, 4005
BstUI CGCG 1 cut(s) 4561
BstV2I GAAGAC 2 cut(s) 2555, 4046
BstX2I RGATCY 2 cut(s) 918, 2244
BstXI CCANNNNNNTGG 1 cut(s) 118
BstYI RGATCY 2 cut(s) 918, 2244
BstZ17I GTATAC 1 cut(s) 1868
BsuI GTATCC 2 cut(s) 3177, 4422
BtgI CCRYGG 1 cut(s) 2289
BtgZI GCGATG 1 cut(s) 1182
BtrI CACGTC 1 cut(s) 4292
BtsI GCAGTG 1 cut(s) 4335
BveI ACCTGC 2 cut(s) 380, 3823
Cac8I GCNNGC 2 cut(s) 3427, 4576
CaiI CAGNNNCTG 2 cut(s) 1772, 1787
CciI TCATGA 2 cut(s) 1584, 4057
CfoI GCGC 2 cut(s) 2421, 3272
Cfr13I GGNCC 8 cut(s) 147, 514, 648, 1437, 1611, 2070, 2341, 4420
CseI GACGC 1 cut(s) 4259
CsiI ACCWGGT 1 cut(s) 787
Csp6I GTAC 4 cut(s) 1570, 1727, 3928, 4044
CspCI CAANNNNNGTGG 4 cut(s) 2383, 2418, 3156, 3191
CviQI GTAC 4 cut(s) 1570, 1727, 3928, 4044
DdeI CTNAG 9 cut(s) 1325, 2140, 2232, 2373, 2399, 3188, 3411, 3604, 3610
DraI TTTAAA 3 cut(s) 672, 1180, 3237
DrdI GACNNNNNNGTC 1 cut(s) 3863
DriI GACNNNNNGTC 1 cut(s) 1206
DseDI GACNNNNNNGTC 1 cut(s) 3863
EaeI YGGCCR 3 cut(s) 2407, 3308, 3946
Eam1104I CTCTTC 5 cut(s) 2829, 3020, 4200, 4209, 4298
Eam1105I GACNNNNNGTC 1 cut(s) 1206
EarI CTCTTC 5 cut(s) 2829, 3020, 4200, 4209, 4298
Ecl136II GAGCTC 1 cut(s) 2537
Eco130I CCWWGG 1 cut(s) 2289
Eco24I GRGCYC 1 cut(s) 2539
Eco31I GGTCTC 1 cut(s) 3683
Eco32I GATATC 3 cut(s) 805, 817, 1299
Eco47I GGWCC 3 cut(s) 147, 1437, 2341
Eco53kI GAGCTC 1 cut(s) 2537
Eco57I CTGAAG 7 cut(s) 1856, 2475, 2793, 2969, 3087, 3701, 4322
Eco72I CACGTG 1 cut(s) 2435
EcoICRI GAGCTC 1 cut(s) 2537
EcoNI CCTNNNNNAGG 1 cut(s) 786
EcoO109I RGGNCCY 2 cut(s) 648, 2341
EcoRII CCWGG 6 cut(s) 516, 688, 787, 1281, 1360, 1812
EcoRV GATATC 3 cut(s) 805, 817, 1299
EcoT14I CCWWGG 1 cut(s) 2289
EcoT22I ATGCAT 1 cut(s) 3774
EcoT38I GRGCYC 1 cut(s) 2539
ErhI CCWWGG 1 cut(s) 2289
FalI AAGNNNNNCTT 2 cut(s) 2301, 2333
FaqI GGGAC 3 cut(s) 2096, 2296, 4302
FauNDI CATATG 2 cut(s) 2224, 3673
FblI GTMKAC 3 cut(s) 1867, 2460, 2577
FriOI GRGCYC 1 cut(s) 2539
FspBI CTAG 9 cut(s) 212, 482, 1026, 1266, 1833, 1853, 2742, 3528, 4319
GlaI GCGC 2 cut(s) 2420, 3271
GsaI CCCAGC 1 cut(s) 3258
GsuI CTGGAG 5 cut(s) 342, 1265, 3471, 4276, 4355
HaeII RGCGCY 1 cut(s) 3273
HapII CCGG 3 cut(s) 102, 2715, 3949
HgaI GACGC 1 cut(s) 4259
HhaI GCGC 2 cut(s) 2421, 3272
Hin1I GRCGYC 1 cut(s) 1899
Hin6I GCGC 2 cut(s) 2419, 3270
HinP1I GCGC 2 cut(s) 2419, 3270
HincII GTYRAC 3 cut(s) 2578, 3567, 4274
HindII GTYRAC 3 cut(s) 2578, 3567, 4274
HpaI GTTAAC 1 cut(s) 3567
HpaII CCGG 3 cut(s) 102, 2715, 3949
HphI GGTGA 5 cut(s) 755, 824, 1376, 1418, 1783
Hpy166II GTNNAC 9 cut(s) 504, 1375, 1440, 1868, 2330, 2461, 2578, 3567, 4274
Hpy8I GTNNAC 9 cut(s) 504, 1375, 1440, 1868, 2330, 2461, 2578, 3567, 4274
Hpy99I CGWCG 1 cut(s) 2651
HpyCH4IV ACGT 6 cut(s) 1847, 1899, 2434, 3574, 4072, 4291
HpyF3I CTNAG 9 cut(s) 1325, 2140, 2232, 2373, 2399, 3188, 3411, 3604, 3610
HpySE526I ACGT 6 cut(s) 1847, 1899, 2434, 3574, 4072, 4291
Hsp92I GRCGYC 1 cut(s) 1899
HspAI GCGC 2 cut(s) 2419, 3270
KspAI GTTAAC 1 cut(s) 3567
MabI ACCWGGT 1 cut(s) 787
MaeI CTAG 9 cut(s) 212, 482, 1026, 1266, 1833, 1853, 2742, 3528, 4319
MaeII ACGT 6 cut(s) 1847, 1899, 2434, 3574, 4072, 4291
MfeI CAATTG 7 cut(s) 39, 944, 995, 1451, 2819, 3738, 3823
MflI RGATCY 2 cut(s) 918, 2244
MhlI GDGCHC 4 cut(s) 967, 2539, 3148, 4262
MlsI TGGCCA 2 cut(s) 2409, 3310
MluNI TGGCCA 2 cut(s) 2409, 3310
MlyI GAGTC 8 cut(s) 1241, 1272, 1323, 1345, 2278, 3015, 3116, 4355
MmeI TCCRAC 3 cut(s) 1950, 3736, 3916
Mox20I TGGCCA 2 cut(s) 2409, 3310
Mph1103I ATGCAT 1 cut(s) 3774
MroXI GAANNNNTTC 4 cut(s) 636, 936, 2626, 3488
MscI TGGCCA 2 cut(s) 2409, 3310
MslI CAYNNNNRTG 2 cut(s) 56, 880
Msp20I TGGCCA 2 cut(s) 2409, 3310
MspA1I CMGCKG 1 cut(s) 1787
MspI CCGG 3 cut(s) 102, 2715, 3949
MspR9I CCNGG 8 cut(s) 102, 518, 690, 789, 1283, 1362, 1814, 2716
MunI CAATTG 7 cut(s) 39, 944, 995, 1451, 2819, 3738, 3823
Mva1269I GAATGC 4 cut(s) 200, 207, 2017, 2421
MvaI CCWGG 6 cut(s) 518, 690, 789, 1283, 1362, 1814
MvnI CGCG 1 cut(s) 4561
NciI CCSGG 2 cut(s) 102, 2716
NcoI CCATGG 1 cut(s) 2289
NdeI CATATG 2 cut(s) 2224, 3673
NlaIV GGNNCC 7 cut(s) 649, 1878, 2181, 2246, 2713, 3454, 4310
NmeAIII GCCGAG 1 cut(s) 3167
NmuCI GTSAC 8 cut(s) 60, 1028, 1233, 1731, 1789, 2000, 3854, 4524
NsiI ATGCAT 1 cut(s) 3774
NspI RCATGY 3 cut(s) 61, 1806, 3772
PagI TCATGA 2 cut(s) 1584, 4057
PciI ACATGT 1 cut(s) 1802
PctI GAATGC 4 cut(s) 200, 207, 2017, 2421
PdmI GAANNNNTTC 4 cut(s) 636, 936, 2626, 3488
PflFI GACNNNGTC 2 cut(s) 2684, 3412
PflMI CCANNNNNTGG 1 cut(s) 3627
PfoI TCCNGGA 2 cut(s) 100, 2714
PleI GAGTC 8 cut(s) 1240, 1272, 1323, 1345, 2278, 3014, 3115, 4355
PmaCI CACGTG 1 cut(s) 2435
PmlI CACGTG 1 cut(s) 2435
PpsI GAGTC 8 cut(s) 1240, 1272, 1323, 1345, 2278, 3014, 3115, 4355
Ppu21I YACGTR 1 cut(s) 2435
PpuMI RGGWCCY 1 cut(s) 2341
PscI ACATGT 1 cut(s) 1802
PshAI GACNNNNGTC 1 cut(s) 1021
PshBI ATTAAT 3 cut(s) 896, 2088, 2954
Psp124BI GAGCTC 1 cut(s) 2539
Psp5II RGGWCCY 1 cut(s) 2341
Psp6I CCWGG 6 cut(s) 516, 688, 787, 1281, 1360, 1812
PspCI CACGTG 1 cut(s) 2435
PspFI CCCAGC 1 cut(s) 3254
PspGI CCWGG 6 cut(s) 516, 688, 787, 1281, 1360, 1812
PspN4I GGNNCC 7 cut(s) 649, 1878, 2181, 2246, 2713, 3454, 4310
PspPI GGNCC 8 cut(s) 147, 514, 648, 1437, 1611, 2070, 2341, 4420
PspPPI RGGWCCY 1 cut(s) 2341
PsrI GAACNNNNNNTAC 2 cut(s) 3282, 3314
PstI CTGCAG 3 cut(s) 1306, 2764, 3793
PstNI CAGNNNCTG 2 cut(s) 1772, 1787
PsuI RGATCY 2 cut(s) 918, 2244
PsyI GACNNNGTC 2 cut(s) 2684, 3412
PvuII CAGCTG 1 cut(s) 1787
RsaI GTAC 4 cut(s) 1571, 1728, 3929, 4045
RsaNI GTAC 4 cut(s) 1570, 1727, 3928, 4044
RseI CAYNNNNRTG 2 cut(s) 56, 880
SacI GAGCTC 1 cut(s) 2539
SalI GTCGAC 1 cut(s) 2576
Sau96I GGNCC 8 cut(s) 147, 514, 648, 1437, 1611, 2070, 2341, 4420
ScaI AGTACT 1 cut(s) 1728
SchI GAGTC 8 cut(s) 1241, 1272, 1323, 1345, 2278, 3015, 3116, 4355
ScrFI CCNGG 8 cut(s) 102, 518, 690, 789, 1283, 1362, 1814, 2716
SduI GDGCHC 4 cut(s) 967, 2539, 3148, 4262
SexAI ACCWGGT 1 cut(s) 787
SfcI CTRYAG 5 cut(s) 1302, 2760, 3274, 3789, 4005
SinI GGWCC 3 cut(s) 147, 1437, 2341
SmiMI CAYNNNNRTG 2 cut(s) 56, 880
SmlI CTYRAG 5 cut(s) 923, 1103, 2318, 2345, 4175
SmoI CTYRAG 5 cut(s) 923, 1103, 2318, 2345, 4175
SpeI ACTAGT 1 cut(s) 4318
SsiI CCGC 1 cut(s) 554
SspI AATATT 4 cut(s) 247, 984, 2272, 2604
SspMI CTAG 9 cut(s) 212, 482, 1026, 1266, 1833, 1853, 2742, 3528, 4319
SstI GAGCTC 1 cut(s) 2539
StyD4I CCNGG 8 cut(s) 100, 516, 688, 787, 1281, 1360, 1812, 2714
StyI CCWWGG 1 cut(s) 2289
TaiI ACGT 6 cut(s) 1850, 1902, 2437, 3577, 4075, 4294
TaqI TCGA 4 cut(s) 961, 2577, 3067, 3219
TatI WGTACW 1 cut(s) 1726
TseFI GTSAC 8 cut(s) 60, 1028, 1233, 1731, 1789, 2000, 3854, 4524
Tsp45I GTSAC 8 cut(s) 60, 1028, 1233, 1731, 1789, 2000, 3854, 4524
TspGWI ACGGA 1 cut(s) 1011
Tth111I GACNNNGTC 2 cut(s) 2684, 3412
Van91I CCANNNNNTGG 1 cut(s) 3627
VpaK11BI GGWCC 3 cut(s) 147, 1437, 2341
VspI ATTAAT 3 cut(s) 896, 2088, 2954
XagI CCTNNNNNAGG 1 cut(s) 786
XceI RCATGY 3 cut(s) 61, 1806, 3772
XcmI CCANNNNNNNNNTGG 1 cut(s) 4342
XmiI GTMKAC 3 cut(s) 1867, 2460, 2577
XmnI GAANNNNTTC 4 cut(s) 636, 936, 2626, 3488
XspI CTAG 9 cut(s) 212, 482, 1026, 1266, 1833, 1853, 2742, 3528, 4319
ZraI GACGTC 1 cut(s) 1900
ZrmI AGTACT 1 cut(s) 1728
Zsp2I ATGCAT 1 cut(s) 3774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.