MD02G1293500.v1.1

Intron-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003959
Physical Location & Seq
Reverse (-)
34891174 .. 34894356
3183 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1293500.v1.1.491

Sequence Viewer

Length: 822 bp
ATGACGAAGGTGTACGGAACAGGGCCGTACGATTTCAAGCGCCACCATGTCGCCGAGTACCCGTTCGAGTACCCGGTCGAGGCCAAGCCCGGTTCGGCGCTTCCTAGCTCGATAACGCTGTTGGATATTCAGCGGGACCGGCTCACGATGATTGCCGCCGATAATTGGTCCAAAACCCTTGACGCCTCGCAGCCCAAGAAGCCCTTCGACCCGGAGCTAGTGAATGAGATTTATCGGACGGAGTTGTCAGTTGGAGAGGGTCAGAGAAGGGCCGTCCCGTCACAGAGGGTGATGATTTTGGAGGTCAGTCAGTACTTGGAGAACTATCTATGGCCAAATTTCGACCCCGAAACCGCAACCTTCGAGCACGTTATGTCCATGATTCTCATTGTCAATGAGAAGTTTCGAGAGAATGTGGCAGCTTGGACGTGCTTTTATGACCGGAAAGATGTGTTCAAAGGATTCCTTGAGAGGGTTCTTCGGCTTAAATCGGGAAGAGAGTTAAGCATAGCTGAAAAGACAAATTATTTAGTTTTCATGATTAATGCCTTTCAGAGTTTGGAAGATGAGATCGTGAGCGATACTGTCCTTAGTTTAGCGAGTTTGCAATCTTGGCACAGTTTGTCGTACGGTCGTTTTCAGATGGAACTTTGTTTTAACCCTGATTTGATCAGGAAATGGAAAAGGATGATAAAGAGGGAGGCCAAAGAGGCTGCAAAACGCGGAGATCCCTTTAATCCCTCAACTACACTCGAAGTTCAGTTCTTGAGAAACCTCATTGAAGAGTTCCTTGAGGTTGTATTTCACACTTTTAAATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
AAA_11 AAA_12 Aquarius_N

Protein Analysis

274

Amino Acids

31.93

Weight (kDa)

6.04

Isoelectric Point (pI)

38.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aquarius_N_1st PF16399 48 - 268 9.3e-67 Intron-binding protein aquarius N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 244
AccII CGCG 1 cut(s) 723
AciI CCGC 4 cut(s) 133, 156, 354, 723
AclWI GGATC 1 cut(s) 722
AcoI YGGCCR 1 cut(s) 332
AcsI RAATTY 2 cut(s) 337, 815
AcyI GRCGYC 1 cut(s) 183
AfaI GTAC 6 cut(s) 14, 29, 59, 71, 314, 629
AfiI CCNNNNNNNGG 3 cut(s) 79, 165, 472
AgsI TTSAA 3 cut(s) 37, 457, 782
AjiI CACGTC 1 cut(s) 429
AjuI GAANNNNNNNTTGG 2 cut(s) 188, 220
AluBI AGCT 4 cut(s) 108, 217, 422, 512
AluI AGCT 4 cut(s) 108, 217, 422, 512
Alw21I GWGCWC 1 cut(s) 369
AlwI GGATC 1 cut(s) 722
AoxI GGCC 5 cut(s) 23, 81, 270, 332, 702
ApeKI GCWGC 3 cut(s) 190, 419, 713
ApoI RAATTY 2 cut(s) 337, 815
AseI ATTAAT 1 cut(s) 543
Asp700I GAANNNNTTC 1 cut(s) 203
AspLEI GCGC 2 cut(s) 42, 100
AspS9I GGNCC 4 cut(s) 23, 136, 168, 270
AsuC2I CCSGG 3 cut(s) 74, 90, 212
AsuHPI GGTGA 1 cut(s) 301
AvaII GGWCC 2 cut(s) 136, 168
BalI TGGCCA 1 cut(s) 334
Bbv12I GWGCWC 1 cut(s) 369
BbvI GCAGC 3 cut(s) 202, 431, 700
BccI CCATC 1 cut(s) 637
BceAI ACGGC 2 cut(s) 10, 257
BclI TGATCA 1 cut(s) 669
BcnI CCSGG 3 cut(s) 74, 90, 212
BfaI CTAG 2 cut(s) 105, 218
BfoI RGCGCY 2 cut(s) 43, 101
BglI GCCNNNNNGGC 1 cut(s) 710
BisI GCNGC 4 cut(s) 156, 191, 420, 714
BlsI GCNGC 4 cut(s) 157, 192, 421, 715
BmcAI AGTACT 1 cut(s) 314
Bme1390I CCNGG 3 cut(s) 74, 90, 212
Bme18I GGWCC 2 cut(s) 136, 168
BmgBI CACGTC 1 cut(s) 429
BmgT120I GGNCC 4 cut(s) 23, 136, 168, 270
BmiI GGNNCC 1 cut(s) 137
BmrFI CCNGG 3 cut(s) 74, 90, 212
BpuEI CTTGAG 3 cut(s) 488, 787, 812
BpuMI CCSGG 3 cut(s) 74, 90, 212
BsaHI GRCGYC 1 cut(s) 183
BsaWI WCCGGW 1 cut(s) 441
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 165, 472
Bse118I RCCGGY 1 cut(s) 138
BseGI GGATG 1 cut(s) 693
BseLI CCNNNNNNNGG 3 cut(s) 79, 165, 472
BseXI GCAGC 3 cut(s) 202, 431, 700
Bsh1236I CGCG 1 cut(s) 723
Bsh1285I CGRYCG 2 cut(s) 78, 634
BshFI GGCC 5 cut(s) 25, 83, 272, 334, 704
BsiEI CGRYCG 2 cut(s) 78, 634
BsiHKAI GWGCWC 1 cut(s) 369
BsiSI CCGG 5 cut(s) 74, 90, 139, 212, 442
BsiWI CGTACG 2 cut(s) 27, 627
BslFI GGGAC 2 cut(s) 149, 260
BslI CCNNNNNNNGG 3 cut(s) 79, 165, 472
BsmFI GGGAC 2 cut(s) 149, 260
BsnI GGCC 5 cut(s) 25, 83, 272, 334, 704
Bsp1286I GDGCHC 1 cut(s) 369
Bsp143I GATC 3 cut(s) 570, 669, 727
BspACI CCGC 4 cut(s) 133, 156, 354, 723
BspANI GGCC 5 cut(s) 25, 83, 272, 334, 704
BspFNI CGCG 1 cut(s) 723
BspHI TCATGA 1 cut(s) 537
BspLI GGNNCC 1 cut(s) 137
BspPI GGATC 1 cut(s) 722
BsrFI RCCGGY 1 cut(s) 138
BssAI RCCGGY 1 cut(s) 138
BssMI GATC 3 cut(s) 570, 669, 727
BssNI GRCGYC 1 cut(s) 183
Bst4CI ACNGT 3 cut(s) 586, 620, 632
Bst6I CTCTTC 2 cut(s) 490, 777
BstACI GRCGYC 1 cut(s) 183
BstDEI CTNAG 1 cut(s) 590
BstF5I GGATG 1 cut(s) 693
BstFNI CGCG 1 cut(s) 723
BstH2I RGCGCY 2 cut(s) 43, 101
BstHHI GCGC 2 cut(s) 42, 100
BstKTI GATC 3 cut(s) 573, 672, 730
BstMBI GATC 3 cut(s) 570, 669, 727
BstMCI CGRYCG 2 cut(s) 78, 634
BstMWI GCNNNNNNNGC 4 cut(s) 139, 199, 613, 710
BstSCI CCNGG 3 cut(s) 72, 88, 210
BstUI CGCG 1 cut(s) 723
BstV1I GCAGC 3 cut(s) 202, 431, 700
BstX2I RGATCY 1 cut(s) 727
BstYI RGATCY 1 cut(s) 727
BsuRI GGCC 5 cut(s) 25, 83, 272, 334, 704
BtrI CACGTC 1 cut(s) 429
BtsCI GGATG 1 cut(s) 693
CciI TCATGA 1 cut(s) 537
CfoI GCGC 2 cut(s) 42, 100
Cfr10I RCCGGY 1 cut(s) 138
Cfr13I GGNCC 4 cut(s) 23, 136, 168, 270
CseI GACGC 1 cut(s) 191
Csp6I GTAC 6 cut(s) 13, 28, 58, 70, 313, 628
CviAII CATG 3 cut(s) 47, 379, 538
CviQI GTAC 6 cut(s) 13, 28, 58, 70, 313, 628
DdeI CTNAG 1 cut(s) 590
DpnI GATC 3 cut(s) 572, 671, 729
DpnII GATC 3 cut(s) 570, 669, 727
DraI TTTAAA 1 cut(s) 814
DrdI GACNNNNNNGTC 1 cut(s) 244
DseDI GACNNNNNNGTC 1 cut(s) 244
EaeI YGGCCR 1 cut(s) 332
Eam1104I CTCTTC 2 cut(s) 490, 777
EarI CTCTTC 2 cut(s) 490, 777
Eco47I GGWCC 2 cut(s) 136, 168
FaeI CATG 3 cut(s) 50, 382, 541
FaiI YATR 7 cut(s) 48, 331, 374, 380, 438, 509, 539
FalI AAGNNNNNCTT 6 cut(s) 188, 220, 450, 482, 774, 806
FaqI GGGAC 2 cut(s) 149, 260
FatI CATG 3 cut(s) 46, 378, 537
FauI CCCGC 1 cut(s) 126
FbaI TGATCA 1 cut(s) 669
Fnu4HI GCNGC 4 cut(s) 156, 191, 420, 714
FokI GGATG 1 cut(s) 700
Fsp4HI GCNGC 4 cut(s) 156, 191, 420, 714
FspBI CTAG 2 cut(s) 105, 218
GlaI GCGC 2 cut(s) 41, 99
GluI GCNGC 4 cut(s) 156, 191, 420, 714
HaeII RGCGCY 2 cut(s) 43, 101
HaeIII GGCC 5 cut(s) 25, 83, 272, 334, 704
HapII CCGG 5 cut(s) 74, 90, 139, 212, 442
HgaI GACGC 1 cut(s) 191
HhaI GCGC 2 cut(s) 42, 100
Hin1I GRCGYC 1 cut(s) 183
Hin1II CATG 3 cut(s) 50, 382, 541
Hin6I GCGC 2 cut(s) 40, 98
HinP1I GCGC 2 cut(s) 40, 98
HinfI GANTC 2 cut(s) 382, 462
HpaII CCGG 5 cut(s) 74, 90, 139, 212, 442
HphI GGTGA 1 cut(s) 301
Hpy166II GTNNAC 1 cut(s) 13
Hpy188I TCNGA 5 cut(s) 237, 264, 555, 642, 821
Hpy188III TCNNGA 7 cut(s) 145, 407, 492, 538, 574, 673, 766
Hpy8I GTNNAC 1 cut(s) 13
HpyAV CCTTC 3 cut(s) 214, 261, 370
HpyCH4III ACNGT 3 cut(s) 586, 620, 632
HpyCH4IV ACGT 2 cut(s) 369, 428
HpyCH4V TGCA 2 cut(s) 607, 716
HpyF10VI GCNNNNNNNGC 4 cut(s) 139, 199, 613, 710
HpyF3I CTNAG 1 cut(s) 590
HpySE526I ACGT 2 cut(s) 369, 428
Hsp92I GRCGYC 1 cut(s) 183
Hsp92II CATG 3 cut(s) 50, 382, 541
HspAI GCGC 2 cut(s) 40, 98
Ksp22I TGATCA 1 cut(s) 669
Kzo9I GATC 3 cut(s) 570, 669, 727
LmnI GCTCC 1 cut(s) 214
LpnPI CCDG 8 cut(s) 6, 87, 103, 152, 225, 455, 658, 675
Lsp1109I GCAGC 3 cut(s) 202, 431, 700
MaeI CTAG 2 cut(s) 105, 218
MaeII ACGT 2 cut(s) 369, 428
MaeIII GTNAC 1 cut(s) 279
MalI GATC 3 cut(s) 572, 671, 729
MboI GATC 3 cut(s) 570, 669, 727
MboII GAAGA 4 cut(s) 470, 507, 575, 794
MflI RGATCY 1 cut(s) 727
MhlI GDGCHC 1 cut(s) 369
MlsI TGGCCA 1 cut(s) 334
MluCI AATT 4 cut(s) 163, 337, 523, 815
MluNI TGGCCA 1 cut(s) 334
MmeI TCCRAC 2 cut(s) 102, 232
Mox20I TGGCCA 1 cut(s) 334
MroXI GAANNNNTTC 1 cut(s) 203
MscI TGGCCA 1 cut(s) 334
MseI TTAA 6 cut(s) 486, 503, 543, 657, 735, 813
Msp20I TGGCCA 1 cut(s) 334
MspA1I CMGCKG 1 cut(s) 133
MspI CCGG 5 cut(s) 74, 90, 139, 212, 442
MspR9I CCNGG 3 cut(s) 74, 90, 212
MvnI CGCG 1 cut(s) 723
MwoI GCNNNNNNNGC 4 cut(s) 139, 199, 613, 710
NciI CCSGG 3 cut(s) 74, 90, 212
NdeII GATC 3 cut(s) 570, 669, 727
NlaIII CATG 3 cut(s) 50, 382, 541
NlaIV GGNNCC 1 cut(s) 137
NmeAIII GCCGAG 1 cut(s) 79
NmuCI GTSAC 1 cut(s) 279
PagI TCATGA 1 cut(s) 537
PdmI GAANNNNTTC 1 cut(s) 203
PfeI GAWTC 2 cut(s) 382, 462
Pfl23II CGTACG 2 cut(s) 27, 627
PkrI GCNGC 4 cut(s) 157, 192, 421, 715
PshBI ATTAAT 1 cut(s) 543
PspLI CGTACG 2 cut(s) 27, 627
PspN4I GGNNCC 1 cut(s) 137
PspPI GGNCC 4 cut(s) 23, 136, 168, 270
PsuI RGATCY 1 cut(s) 727
RsaI GTAC 6 cut(s) 14, 29, 59, 71, 314, 629
RsaNI GTAC 6 cut(s) 13, 28, 58, 70, 313, 628
SaqAI TTAA 6 cut(s) 486, 503, 543, 657, 735, 813
SatI GCNGC 4 cut(s) 156, 191, 420, 714
Sau3AI GATC 3 cut(s) 570, 669, 727
Sau96I GGNCC 4 cut(s) 23, 136, 168, 270
ScaI AGTACT 1 cut(s) 314
ScrFI CCNGG 3 cut(s) 74, 90, 212
SduI GDGCHC 1 cut(s) 369
SinI GGWCC 2 cut(s) 136, 168
SmlI CTYRAG 3 cut(s) 467, 766, 791
SmoI CTYRAG 3 cut(s) 467, 766, 791
Sse9I AATT 4 cut(s) 163, 337, 523, 815
SsiI CCGC 4 cut(s) 133, 156, 354, 723
SspMI CTAG 2 cut(s) 105, 218
StyD4I CCNGG 3 cut(s) 72, 88, 210
TaaI ACNGT 3 cut(s) 586, 620, 632
TaiI ACGT 2 cut(s) 372, 431
TaqI TCGA 8 cut(s) 66, 78, 110, 207, 342, 363, 406, 753
TasI AATT 4 cut(s) 163, 337, 523, 815
TatI WGTACW 1 cut(s) 312
TauI GCSGC 1 cut(s) 158
TfiI GAWTC 2 cut(s) 382, 462
Tru1I TTAA 6 cut(s) 486, 503, 543, 657, 735, 813
Tru9I TTAA 6 cut(s) 486, 503, 543, 657, 735, 813
TseFI GTSAC 1 cut(s) 279
TseI GCWGC 3 cut(s) 190, 419, 713
Tsp45I GTSAC 1 cut(s) 279
TspDTI ATGAA 1 cut(s) 526
TspGWI ACGGA 2 cut(s) 30, 254
VpaK11BI GGWCC 2 cut(s) 136, 168
VspI ATTAAT 1 cut(s) 543
XapI RAATTY 2 cut(s) 337, 815
XmnI GAANNNNTTC 1 cut(s) 203
XspI CTAG 2 cut(s) 105, 218
ZrmI AGTACT 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.