MD03G1014200.v1.1

Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003999
Physical Location & Seq
Reverse (-)
1111772 .. 1113496
1725 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1014200.v1.1.491

Sequence Viewer

Length: 975 bp
ATGTCTTTATTTTCAATTGCAGCAGCAATATTTGTTCTTGTTACTGCTACGATATTGGGAAATTCCAACGCTCAACTGAGCTCAACTTTCTACAGCACCACATGCCCGAATGTGACGAGCATTGTTCGTGGTGTGGTCGAGCAAGCTCAACAGAATGACATCCGAATTGGTGCCAAACTCATCCGAGTTCATTTCCACGACTGCTTTGTCAACGGTTGTGATGGATCGATTATGCTGGACAACACAGATGGGATAGAGAGCGAGAAAGATGCACCTCCCAATCAATCGACAGACGGGTACGACGTTGTAGATGATATCAAAACTGCGGTCGAGAATGTTTGCCCTGGTGTTGTCTCCTGTTCTGACATTTTAGCAATTGCTTCTCAAATTCTTGTTTCTGCGAATGGAGGGCCAACGTGGGAAGCTCAATTGGGAAGAAGAGACAGTAGGACAGCCAACCGAGCTGGTACCAGTGCCATTCCAAGCTTTCTCGAAAACCTTGAGCAAATTTCACAAAAATTTCGCGATGCAGGATTAGATTCCACTGATCTGGTTGCTTTATCAGGTGCTCATACATTTGGACGGGCAAGATGTTCAACTTTCGTTCACCGCCTCTACAATTTCAGCGGCACTGGCAACCCGGACCCGACCATTGAAGCCGGATACTTGGAAACCCTCCGCCAAGCATGTCCTCAAAACGGCAGCGGAGATACGTTGAATGACCTTGACCAGTCCACCCGCGATGCCTTCGACAACCACTACTTTACAAATCTCCAAAACAACCGCGGGCTTCTCCAGACCGATCAGGTGCTGTTCTCAACTAGCGGAGACACCGTTGCCATTGTGGACCGCTTTGCAAATAGCCAAAGTGACTTCTTTGATAGCTTTGGTCAGTCCATGATCAATATGGGGAATATAGGAGTTTTGACGGGCACCAATGGAGAGATTAGGACTGATTGCAGAAGGGTTAATTAA

Protein Analysis

325

Amino Acids

34.89

Weight (kDa)

4.6

Isoelectric Point (pI)

36.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 42 - 288 1.4e-83 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 206
Acc65I GGTACC 1 cut(s) 467
AccB1I GGYRCC 3 cut(s) 170, 467, 932
AccII CGCG 3 cut(s) 525, 741, 786
AclWI GGATC 1 cut(s) 232
AcsI RAATTY 4 cut(s) 61, 387, 507, 518
AfaI GTAC 2 cut(s) 299, 469
AfiI CCNNNNNNNGG 1 cut(s) 698
AgsI TTSAA 4 cut(s) 15, 597, 656, 718
AjnI CCWGG 1 cut(s) 343
AluBI AGCT 6 cut(s) 81, 146, 425, 464, 486, 885
AluI AGCT 6 cut(s) 81, 146, 425, 464, 486, 885
Alw21I GWGCWC 2 cut(s) 83, 571
Alw26I GTCTC 3 cut(s) 358, 435, 822
AlwI GGATC 1 cut(s) 232
AlwNI CAGNNNCTG 1 cut(s) 811
AoxI GGCC 1 cut(s) 410
ApeKI GCWGC 3 cut(s) 20, 23, 702
ApoI RAATTY 4 cut(s) 61, 387, 507, 518
ArsI GACNNNNNNTTYG 2 cut(s) 312, 344
Asp718I GGTACC 1 cut(s) 467
AspS9I GGNCC 3 cut(s) 410, 643, 847
AsuC2I CCSGG 1 cut(s) 641
AsuHPI GGTGA 1 cut(s) 599
AvaII GGWCC 2 cut(s) 643, 847
BaeGI GKGCMC 1 cut(s) 935
BanI GGYRCC 3 cut(s) 170, 467, 932
BanII GRGCYC 1 cut(s) 83
Bbv12I GWGCWC 2 cut(s) 83, 571
BbvI GCAGC 3 cut(s) 32, 35, 714
BccI CCATC 2 cut(s) 215, 242
BceAI ACGGC 1 cut(s) 715
BcgI CGANNNNNNTGC 2 cut(s) 251, 285
BciT130I CCWGG 1 cut(s) 345
BciVI GTATCC 1 cut(s) 656
BclI TGATCA 1 cut(s) 900
BcnI CCSGG 1 cut(s) 641
BcoDI GTCTC 3 cut(s) 358, 435, 822
BfaI CTAG 1 cut(s) 822
BfmI CTRYAG 1 cut(s) 91
BfuI GTATCC 1 cut(s) 656
BisI GCNGC 4 cut(s) 21, 24, 628, 703
BlsI GCNGC 4 cut(s) 22, 25, 629, 704
Bme1390I CCNGG 2 cut(s) 345, 641
Bme18I GGWCC 2 cut(s) 643, 847
BmgT120I GGNCC 3 cut(s) 410, 643, 847
BmiI GGNNCC 4 cut(s) 172, 469, 645, 934
BmrFI CCNGG 2 cut(s) 345, 641
BmsI GCATC 3 cut(s) 259, 517, 733
BpmI CTGGAG 1 cut(s) 779
BpuEI CTTGAG 1 cut(s) 521
BpuMI CCSGG 1 cut(s) 641
Bsa29I ATCGAT 1 cut(s) 227
BsaJI CCNNGG 2 cut(s) 343, 784
BsaXI ACNNNNNCTCC 2 cut(s) 819, 849
Bsc4I CCNNNNNNNGG 1 cut(s) 698
Bse1I ACTGG 3 cut(s) 471, 637, 730
BseBI CCWGG 1 cut(s) 345
BseCI ATCGAT 1 cut(s) 227
BseDI CCNNGG 2 cut(s) 343, 784
BseGI GGATG 2 cut(s) 159, 180
BseLI CCNNNNNNNGG 1 cut(s) 698
BseMII CTCAG 1 cut(s) 68
BseNI ACTGG 3 cut(s) 471, 637, 730
BseSI GKGCMC 1 cut(s) 935
BseXI GCAGC 3 cut(s) 32, 35, 714
Bsh1236I CGCG 3 cut(s) 525, 741, 786
Bsh1285I CGRYCG 1 cut(s) 330
BshFI GGCC 1 cut(s) 412
BshNI GGYRCC 3 cut(s) 170, 467, 932
BshVI ATCGAT 1 cut(s) 227
BsiEI CGRYCG 1 cut(s) 330
BsiHKAI GWGCWC 2 cut(s) 83, 571
BsiSI CCGG 2 cut(s) 641, 660
BslI CCNNNNNNNGG 1 cut(s) 698
BsmAI GTCTC 3 cut(s) 358, 435, 822
BsnI GGCC 1 cut(s) 412
Bsp1286I GDGCHC 3 cut(s) 83, 571, 935
Bsp143I GATC 4 cut(s) 224, 547, 802, 900
Bsp68I TCGCGA 1 cut(s) 525
BspANI GGCC 1 cut(s) 412
BspCNI CTCAG 1 cut(s) 69
BspDI ATCGAT 1 cut(s) 227
BspFNI CGCG 3 cut(s) 525, 741, 786
BspLI GGNNCC 4 cut(s) 172, 469, 645, 934
BspPI GGATC 1 cut(s) 232
BspT107I GGYRCC 3 cut(s) 170, 467, 932
BsrI ACTGG 3 cut(s) 471, 637, 730
BssECI CCNNGG 2 cut(s) 343, 784
BssMI GATC 4 cut(s) 224, 547, 802, 900
Bst2UI CCWGG 1 cut(s) 345
Bst4CI ACNGT 3 cut(s) 215, 446, 835
Bst6I CTCTTC 1 cut(s) 433
BstAPI GCANNNNNTGC 1 cut(s) 102
BstC8I GCNNGC 2 cut(s) 144, 788
BstDEI CTNAG 1 cut(s) 77
BstDSI CCRYGG 1 cut(s) 784
BstF5I GGATG 2 cut(s) 159, 180
BstFNI CGCG 3 cut(s) 525, 741, 786
BstKTI GATC 4 cut(s) 227, 550, 805, 903
BstMAI GTCTC 3 cut(s) 358, 435, 822
BstMBI GATC 4 cut(s) 224, 547, 802, 900
BstMCI CGRYCG 1 cut(s) 330
BstMWI GCNNNNNNNGC 3 cut(s) 102, 461, 633
BstNI CCWGG 1 cut(s) 345
BstNSI RCATGY 2 cut(s) 105, 690
BstSCI CCNGG 2 cut(s) 343, 639
BstSFI CTRYAG 1 cut(s) 91
BstSLI GKGCMC 1 cut(s) 935
BstUI CGCG 3 cut(s) 525, 741, 786
BstV1I GCAGC 3 cut(s) 32, 35, 714
BstXI CCANNNNNNTGG 1 cut(s) 550
Bsu15I ATCGAT 1 cut(s) 227
BsuI GTATCC 1 cut(s) 656
BsuRI GGCC 1 cut(s) 412
BsuTUI ATCGAT 1 cut(s) 227
BtgI CCRYGG 1 cut(s) 784
BtgZI GCGATG 2 cut(s) 540, 756
BtsCI GGATG 2 cut(s) 159, 180
BtsIMutI CAGTG 3 cut(s) 478, 543, 630
BtuMI TCGCGA 1 cut(s) 525
Cac8I GCNNGC 2 cut(s) 144, 788
CaiI CAGNNNCTG 1 cut(s) 811
Cfr13I GGNCC 3 cut(s) 410, 643, 847
Cfr42I CCGCGG 1 cut(s) 787
ClaI ATCGAT 1 cut(s) 227
Csp6I GTAC 2 cut(s) 298, 468
CviAII CATG 3 cut(s) 102, 687, 898
CviQI GTAC 2 cut(s) 298, 468
DdeI CTNAG 1 cut(s) 77
DpnI GATC 4 cut(s) 226, 549, 804, 902
DpnII GATC 4 cut(s) 224, 547, 802, 900
DrdI GACNNNNNNGTC 1 cut(s) 206
DseDI GACNNNNNNGTC 1 cut(s) 206
Eam1104I CTCTTC 1 cut(s) 433
EarI CTCTTC 1 cut(s) 433
EciI GGCGGA 1 cut(s) 668
Ecl136II GAGCTC 1 cut(s) 81
Eco24I GRGCYC 1 cut(s) 83
Eco32I GATATC 1 cut(s) 316
Eco47I GGWCC 2 cut(s) 643, 847
Eco53kI GAGCTC 1 cut(s) 81
EcoICRI GAGCTC 1 cut(s) 81
EcoRII CCWGG 1 cut(s) 343
EcoRV GATATC 1 cut(s) 316
EcoT38I GRGCYC 1 cut(s) 83
FaeI CATG 3 cut(s) 105, 690, 901
FaiI YATR 7 cut(s) 103, 233, 573, 688, 899, 908, 917
FatI CATG 3 cut(s) 101, 686, 897
FauI CCCGC 2 cut(s) 746, 779
FbaI TGATCA 1 cut(s) 900
Fnu4HI GCNGC 4 cut(s) 21, 24, 628, 703
FokI GGATG 2 cut(s) 146, 167
FriOI GRGCYC 1 cut(s) 83
Fsp4HI GCNGC 4 cut(s) 21, 24, 628, 703
FspBI CTAG 1 cut(s) 822
GluI GCNGC 4 cut(s) 21, 24, 628, 703
GsuI CTGGAG 1 cut(s) 779
HaeIII GGCC 1 cut(s) 412
HapII CCGG 2 cut(s) 641, 660
Hin1II CATG 3 cut(s) 105, 690, 901
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HindIII AAGCTT 1 cut(s) 484
HinfI GANTC 1 cut(s) 539
HpaII CCGG 2 cut(s) 641, 660
HphI GGTGA 1 cut(s) 599
Hpy166II GTNNAC 4 cut(s) 211, 607, 735, 847
Hpy188I TCNGA 3 cut(s) 164, 185, 364
Hpy188III TCNNGA 4 cut(s) 331, 491, 524, 796
Hpy8I GTNNAC 4 cut(s) 211, 607, 735, 847
Hpy99I CGWCG 1 cut(s) 305
HpyAV CCTTC 2 cut(s) 757, 957
HpyCH4III ACNGT 3 cut(s) 215, 446, 835
HpyCH4IV ACGT 3 cut(s) 303, 416, 713
HpyCH4V TGCA 5 cut(s) 20, 272, 530, 857, 960
HpyF10VI GCNNNNNNNGC 3 cut(s) 102, 461, 633
HpyF3I CTNAG 1 cut(s) 77
HpySE526I ACGT 3 cut(s) 303, 416, 713
Hsp92II CATG 3 cut(s) 105, 690, 901
KpnI GGTACC 1 cut(s) 471
Ksp22I TGATCA 1 cut(s) 900
KspI CCGCGG 1 cut(s) 787
Kzo9I GATC 4 cut(s) 224, 547, 802, 900
Lsp1109I GCAGC 3 cut(s) 32, 35, 714
LweI GCATC 3 cut(s) 259, 517, 733
MaeI CTAG 1 cut(s) 822
MaeII ACGT 3 cut(s) 303, 416, 713
MaeIII GTNAC 3 cut(s) 40, 112, 869
MalI GATC 4 cut(s) 226, 549, 804, 902
MboI GATC 4 cut(s) 224, 547, 802, 900
MboII GAAGA 2 cut(s) 447, 450
MfeI CAATTG 3 cut(s) 15, 375, 428
MhlI GDGCHC 3 cut(s) 83, 571, 935
MmeI TCCRAC 1 cut(s) 90
MnlI CCTC 5 cut(s) 285, 401, 623, 686, 702
MseI TTAA 2 cut(s) 969, 973
MspA1I CMGCKG 3 cut(s) 627, 705, 786
MspI CCGG 2 cut(s) 641, 660
MspR9I CCNGG 2 cut(s) 345, 641
MunI CAATTG 3 cut(s) 15, 375, 428
MvaI CCWGG 1 cut(s) 345
MvnI CGCG 3 cut(s) 525, 741, 786
MwoI GCNNNNNNNGC 3 cut(s) 102, 461, 633
NciI CCSGG 1 cut(s) 641
NdeII GATC 4 cut(s) 224, 547, 802, 900
NlaIII CATG 3 cut(s) 105, 690, 901
NlaIV GGNNCC 4 cut(s) 172, 469, 645, 934
NmuCI GTSAC 2 cut(s) 112, 869
NruI TCGCGA 1 cut(s) 525
NspI RCATGY 2 cut(s) 105, 690
PacI TTAATTAA 1 cut(s) 973
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PfeI GAWTC 1 cut(s) 539
PkrI GCNGC 4 cut(s) 22, 25, 629, 704
Psp124BI GAGCTC 1 cut(s) 83
Psp6I CCWGG 1 cut(s) 343
PspGI CCWGG 1 cut(s) 343
PspN4I GGNNCC 4 cut(s) 172, 469, 645, 934
PspPI GGNCC 3 cut(s) 410, 643, 847
PstNI CAGNNNCTG 1 cut(s) 811
RruI TCGCGA 1 cut(s) 525
RsaI GTAC 2 cut(s) 299, 469
RsaNI GTAC 2 cut(s) 298, 468
SacI GAGCTC 1 cut(s) 83
SacII CCGCGG 1 cut(s) 787
SaqAI TTAA 2 cut(s) 969, 973
SatI GCNGC 4 cut(s) 21, 24, 628, 703
Sau3AI GATC 4 cut(s) 224, 547, 802, 900
Sau96I GGNCC 3 cut(s) 410, 643, 847
ScrFI CCNGG 2 cut(s) 345, 641
SduI GDGCHC 3 cut(s) 83, 571, 935
SfaNI GCATC 3 cut(s) 259, 517, 733
SfcI CTRYAG 1 cut(s) 91
Sfr303I CCGCGG 1 cut(s) 787
SgrBI CCGCGG 1 cut(s) 787
SinI GGWCC 2 cut(s) 643, 847
SmlI CTYRAG 1 cut(s) 500
SmoI CTYRAG 1 cut(s) 500
SspI AATATT 1 cut(s) 30
SspMI CTAG 1 cut(s) 822
SstI GAGCTC 1 cut(s) 83
StyD4I CCNGG 2 cut(s) 343, 639
TaaI ACNGT 3 cut(s) 215, 446, 835
TaiI ACGT 3 cut(s) 306, 419, 716
TaqI TCGA 6 cut(s) 138, 227, 287, 330, 492, 750
TaqII GACCGA 1 cut(s) 815
TauI GCSGC 1 cut(s) 630
TfiI GAWTC 1 cut(s) 539
Tru1I TTAA 2 cut(s) 969, 973
Tru9I TTAA 2 cut(s) 969, 973
TscAI CASTG 3 cut(s) 478, 550, 637
TseFI GTSAC 2 cut(s) 112, 869
TseI GCWGC 3 cut(s) 20, 23, 702
Tsp45I GTSAC 2 cut(s) 112, 869
TspDTI ATGAA 1 cut(s) 179
TspRI CASTG 3 cut(s) 478, 550, 637
VpaK11BI GGWCC 2 cut(s) 643, 847
XapI RAATTY 4 cut(s) 61, 387, 507, 518
XceI RCATGY 2 cut(s) 105, 690
XcmI CCANNNNNNNNNTGG 1 cut(s) 904
XspI CTAG 1 cut(s) 822
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.