Rw5G049270

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0003999
Physical Location & Seq
Forward (+)
85366097 .. 85368016
1920 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G049270.1

Sequence Viewer

Length: 915 bp
ATGTCTACTTTTTCATATGTTGCAGCAGCAATTGTTCTCTTTACCATTGTGTTGGGCAACTCCAATGCTCAGCTCAGCTCCACTTTTTACGACACAACGTGCCCTAATGTGTCGAGCGTGGTTCGTAATGTGGTGGAGCAAGCTCAACAGAATGATATTCGTATTGGCGCCAAACTCATCCGGGTTCACTTCCACGACTGCTTCGTCAATGGGTGTGATGCATCACTTTTGCTCGACGATGCAGACGACATAGACAGTGAAAAAAATGCAAATGTTTGCCCTGGTGTTGTCTCCTGTGCTGATATTGTAGCTCTTGCTTCTCAGATACTCGTTTCTGAGAATGGAGGACCAACTTGGGAAGTTCAATTGGGAAGAAGAGATAGTAGGACAGCCAACCGGGCTGGTACTACTGCCATTCCAAGCCCTTTTGAAACCCTTGCGCAAATTACCAAAAAGTTCAGTGATGCAGGCCTTGATTCCACAGATCTCGTTGCTTTGTCAGGTGCTCATACATTTGGCCGGGCACAGTGTTCAACTTTCGTCCACCGCCTGTACAATTTCAGTGGTACGGGGAACCCCGACCCGAGCCTGGACACAACCTACTTGGAAACCCTACGCCAAACATGCCCCGAAGGCGGCAATGGAGGTACATTGGCCAACCTTGATCCTTCTACGCCCAATGGCTTCGACAACAACTACTTCACAAACCTTCAGAACAAGCAGGGGCTTCTCCAGACTGATCAGGAGCTGTTTTCGACCAGTGGAGCCGACACTAGTTCCATTGTGGACCGGTTTGCTAATAGCCAAAGCGACTTCTTTGACACTTTCGCTAAGTCCATGACCAATATGGGAAATATAAAACCTTTGACGGGCAGTGATGGAGAAATTAGGTCTGATTGTAAAAGAGTTAACTAA

Protein Analysis

304

Amino Acids

32.61

Weight (kDa)

4.61

Isoelectric Point (pI)

33.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 41 - 268 7.8e-71 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 203
Acc16I TGCGCA 1 cut(s) 441
AccB1I GGYRCC 1 cut(s) 167
AccI GTMKAC 1 cut(s) 5
AciI CCGC 2 cut(s) 547, 636
AclWI GGATC 1 cut(s) 659
AcoI YGGCCR 2 cut(s) 517, 654
AcuI CTGAAG 1 cut(s) 695
AcyI GRCGYC 1 cut(s) 168
AdeI CACNNNGTG 1 cut(s) 99
AfaI GTAC 4 cut(s) 406, 554, 568, 649
AfiI CCNNNNNNNGG 3 cut(s) 589, 635, 869
AgeI ACCGGT 1 cut(s) 789
AgsI TTSAA 3 cut(s) 365, 431, 534
AhlI ACTAGT 1 cut(s) 773
AjnI CCWGG 2 cut(s) 280, 588
AluBI AGCT 5 cut(s) 73, 78, 143, 311, 748
AluI AGCT 5 cut(s) 73, 78, 143, 311, 748
Alw21I GWGCWC 1 cut(s) 508
Alw26I GTCTC 1 cut(s) 295
AlwI GGATC 1 cut(s) 659
AlwNI CAGNNNCTG 1 cut(s) 748
Ama87I CYCGRG 1 cut(s) 583
AoxI GGCC 3 cut(s) 469, 517, 654
ApeKI GCWGC 2 cut(s) 23, 26
AsiGI ACCGGT 1 cut(s) 789
AspLEI GCGC 2 cut(s) 170, 442
AspS9I GGNCC 2 cut(s) 347, 787
AsuC2I CCSGG 3 cut(s) 182, 398, 521
AvaI CYCGRG 1 cut(s) 583
AvaII GGWCC 2 cut(s) 347, 787
BaeGI GKGCMC 2 cut(s) 104, 526
BalI TGGCCA 1 cut(s) 656
BanI GGYRCC 1 cut(s) 167
Bbv12I GWGCWC 1 cut(s) 508
BbvI GCAGC 2 cut(s) 35, 38
BccI CCATC 1 cut(s) 872
BciT130I CCWGG 2 cut(s) 282, 590
BclI TGATCA 1 cut(s) 739
BcnI CCSGG 3 cut(s) 182, 398, 521
BcoDI GTCTC 1 cut(s) 295
BcuI ACTAGT 1 cut(s) 773
BfaI CTAG 1 cut(s) 774
BfoI RGCGCY 1 cut(s) 171
BglI GCCNNNNNGGC 2 cut(s) 398, 633
BglII AGATCT 1 cut(s) 484
BisI GCNGC 3 cut(s) 24, 27, 637
BlpI GCTNAGC 2 cut(s) 69, 74
BlsI GCNGC 3 cut(s) 25, 28, 638
Bme1390I CCNGG 5 cut(s) 182, 282, 398, 521, 590
Bme18I GGWCC 2 cut(s) 347, 787
BmeT110I CYCGRG 1 cut(s) 583
BmgT120I GGNCC 2 cut(s) 347, 787
BmiI GGNNCC 3 cut(s) 169, 575, 766
BmrFI CCNGG 5 cut(s) 182, 282, 398, 521, 590
BmsI GCATC 4 cut(s) 208, 229, 230, 454
BpmI CTGGAG 1 cut(s) 716
Bpu1102I GCTNAGC 2 cut(s) 69, 74
BpuMI CCSGG 3 cut(s) 182, 398, 521
BsaHI GRCGYC 1 cut(s) 168
BsaJI CCNNGG 1 cut(s) 280
BsaWI WCCGGW 1 cut(s) 789
Bsc4I CCNNNNNNNGG 3 cut(s) 589, 635, 869
Bse118I RCCGGY 1 cut(s) 789
Bse1I ACTGG 1 cut(s) 759
Bse3DI GCAATG 1 cut(s) 646
BseBI CCWGG 2 cut(s) 282, 590
BseDI CCNNGG 1 cut(s) 280
BseGI GGATG 1 cut(s) 177
BseLI CCNNNNNNNGG 3 cut(s) 589, 635, 869
BseMI GCAATG 1 cut(s) 646
BseMII CTCAG 4 cut(s) 83, 88, 327, 335
BseNI ACTGG 1 cut(s) 759
BseSI GKGCMC 2 cut(s) 104, 526
BseXI GCAGC 2 cut(s) 35, 38
BshFI GGCC 3 cut(s) 471, 519, 656
BshNI GGYRCC 1 cut(s) 167
BshTI ACCGGT 1 cut(s) 789
BsiHKAI GWGCWC 1 cut(s) 508
BsiHKCI CYCGRG 1 cut(s) 583
BsiSI CCGG 4 cut(s) 181, 397, 520, 790
BslI CCNNNNNNNGG 3 cut(s) 589, 635, 869
BsmAI GTCTC 1 cut(s) 295
BsnI GGCC 3 cut(s) 471, 519, 656
BsoBI CYCGRG 1 cut(s) 583
Bsp1286I GDGCHC 3 cut(s) 104, 508, 526
Bsp1407I TGTACA 1 cut(s) 552
Bsp143I GATC 3 cut(s) 484, 664, 739
Bsp1720I GCTNAGC 2 cut(s) 69, 74
BspACI CCGC 2 cut(s) 547, 636
BspANI GGCC 3 cut(s) 471, 519, 656
BspCNI CTCAG 4 cut(s) 82, 87, 328, 334
BspLI GGNNCC 3 cut(s) 169, 575, 766
BspPI GGATC 1 cut(s) 659
BspT107I GGYRCC 1 cut(s) 167
BsrDI GCAATG 1 cut(s) 646
BsrFI RCCGGY 1 cut(s) 789
BsrGI TGTACA 1 cut(s) 552
BsrI ACTGG 1 cut(s) 759
BssAI RCCGGY 1 cut(s) 789
BssECI CCNNGG 1 cut(s) 280
BssMI GATC 3 cut(s) 484, 664, 739
BssNI GRCGYC 1 cut(s) 168
Bst2UI CCWGG 2 cut(s) 282, 590
Bst4CI ACNGT 2 cut(s) 257, 528
Bst6I CTCTTC 1 cut(s) 370
BstACI GRCGYC 1 cut(s) 168
BstAUI TGTACA 1 cut(s) 552
BstC8I GCNNGC 2 cut(s) 141, 469
BstDEI CTNAG 5 cut(s) 69, 74, 321, 336, 831
BstF5I GGATG 1 cut(s) 177
BstH2I RGCGCY 1 cut(s) 171
BstHHI GCGC 2 cut(s) 170, 442
BstKTI GATC 3 cut(s) 487, 667, 742
BstMAI GTCTC 1 cut(s) 295
BstMBI GATC 3 cut(s) 484, 664, 739
BstMWI GCNNNNNNNGC 3 cut(s) 398, 624, 633
BstNI CCWGG 2 cut(s) 282, 590
BstNSI RCATGY 1 cut(s) 627
BstSCI CCNGG 5 cut(s) 180, 280, 396, 519, 588
BstSLI GKGCMC 2 cut(s) 104, 526
BstV1I GCAGC 2 cut(s) 35, 38
BstX2I RGATCY 1 cut(s) 484
BstXI CCANNNNNNTGG 1 cut(s) 52
BstYI RGATCY 1 cut(s) 484
BsuRI GGCC 3 cut(s) 471, 519, 656
BtsCI GGATG 1 cut(s) 177
BtsI GCAGTG 1 cut(s) 880
BtsIMutI CAGTG 6 cut(s) 262, 466, 533, 568, 766, 880
Cac8I GCNNGC 2 cut(s) 141, 469
CaiI CAGNNNCTG 1 cut(s) 748
CfoI GCGC 2 cut(s) 170, 442
Cfr10I RCCGGY 1 cut(s) 789
Cfr13I GGNCC 2 cut(s) 347, 787
Csp6I GTAC 4 cut(s) 405, 553, 567, 648
CspAI ACCGGT 1 cut(s) 789
CspCI CAANNNNNGTGG 2 cut(s) 544, 579
CviAII CATG 2 cut(s) 624, 838
CviQI GTAC 4 cut(s) 405, 553, 567, 648
DdeI CTNAG 5 cut(s) 69, 74, 321, 336, 831
DinI GGCGCC 1 cut(s) 169
DpnI GATC 3 cut(s) 486, 666, 741
DpnII GATC 3 cut(s) 484, 664, 739
DraIII CACNNNGTG 1 cut(s) 99
DrdI GACNNNNNNGTC 1 cut(s) 203
DseDI GACNNNNNNGTC 1 cut(s) 203
EaeI YGGCCR 2 cut(s) 517, 654
Eam1104I CTCTTC 1 cut(s) 370
EarI CTCTTC 1 cut(s) 370
Eco147I AGGCCT 1 cut(s) 471
Eco47I GGWCC 2 cut(s) 347, 787
Eco57I CTGAAG 1 cut(s) 695
Eco88I CYCGRG 1 cut(s) 583
EcoRII CCWGG 2 cut(s) 280, 588
EcoT22I ATGCAT 1 cut(s) 223
EgeI GGCGCC 1 cut(s) 169
EheI GGCGCC 1 cut(s) 169
FaeI CATG 2 cut(s) 627, 841
FaiI YATR 8 cut(s) 16, 18, 251, 510, 625, 839, 848, 857
FatI CATG 2 cut(s) 623, 837
FauNDI CATATG 1 cut(s) 16
FbaI TGATCA 1 cut(s) 739
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 3 cut(s) 24, 27, 637
FokI GGATG 1 cut(s) 164
Fsp4HI GCNGC 3 cut(s) 24, 27, 637
FspBI CTAG 1 cut(s) 774
FspI TGCGCA 1 cut(s) 441
GlaI GCGC 2 cut(s) 169, 441
GluI GCNGC 3 cut(s) 24, 27, 637
GsuI CTGGAG 1 cut(s) 716
HaeII RGCGCY 1 cut(s) 171
HaeIII GGCC 3 cut(s) 471, 519, 656
HapII CCGG 4 cut(s) 181, 397, 520, 790
HhaI GCGC 2 cut(s) 170, 442
Hin1I GRCGYC 1 cut(s) 168
Hin1II CATG 2 cut(s) 627, 841
Hin6I GCGC 2 cut(s) 168, 440
HinP1I GCGC 2 cut(s) 168, 440
HincII GTYRAC 1 cut(s) 910
HindII GTYRAC 1 cut(s) 910
HinfI GANTC 1 cut(s) 476
HpaI GTTAAC 1 cut(s) 910
HpaII CCGG 4 cut(s) 181, 397, 520, 790
Hpy166II GTNNAC 5 cut(s) 6, 187, 544, 787, 910
Hpy188I TCNGA 4 cut(s) 324, 337, 714, 895
Hpy188III TCNNGA 2 cut(s) 733, 743
Hpy8I GTNNAC 5 cut(s) 6, 187, 544, 787, 910
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 3 cut(s) 626, 678, 719
HpyCH4III ACNGT 2 cut(s) 257, 528
HpyCH4IV ACGT 1 cut(s) 98
HpyCH4V TGCA 5 cut(s) 23, 221, 242, 269, 467
HpyF10VI GCNNNNNNNGC 3 cut(s) 398, 624, 633
HpyF3I CTNAG 5 cut(s) 69, 74, 321, 336, 831
HpySE526I ACGT 1 cut(s) 98
Hsp92I GRCGYC 1 cut(s) 168
Hsp92II CATG 2 cut(s) 627, 841
HspAI GCGC 2 cut(s) 168, 440
KasI GGCGCC 1 cut(s) 167
Ksp22I TGATCA 1 cut(s) 739
KspAI GTTAAC 1 cut(s) 910
Kzo9I GATC 3 cut(s) 484, 664, 739
LmnI GCTCC 4 cut(s) 83, 136, 745, 764
Lsp1109I GCAGC 2 cut(s) 35, 38
LweI GCATC 4 cut(s) 208, 229, 230, 454
MaeI CTAG 1 cut(s) 774
MaeII ACGT 1 cut(s) 98
MalI GATC 3 cut(s) 486, 666, 741
MboI GATC 3 cut(s) 484, 664, 739
MboII GAAGA 2 cut(s) 384, 387
MfeI CAATTG 2 cut(s) 30, 365
MflI RGATCY 1 cut(s) 484
MhlI GDGCHC 3 cut(s) 104, 508, 526
MlsI TGGCCA 1 cut(s) 656
MluCI AATT 5 cut(s) 30, 365, 444, 556, 885
MluNI TGGCCA 1 cut(s) 656
Mly113I GGCGCC 1 cut(s) 168
MnlI CCTC 2 cut(s) 338, 638
Mox20I TGGCCA 1 cut(s) 656
Mph1103I ATGCAT 1 cut(s) 223
MscI TGGCCA 1 cut(s) 656
MseI TTAA 1 cut(s) 909
Msp20I TGGCCA 1 cut(s) 656
MspI CCGG 4 cut(s) 181, 397, 520, 790
MspR9I CCNGG 5 cut(s) 182, 282, 398, 521, 590
MunI CAATTG 2 cut(s) 30, 365
MvaI CCWGG 2 cut(s) 282, 590
MwoI GCNNNNNNNGC 3 cut(s) 398, 624, 633
NarI GGCGCC 1 cut(s) 168
NciI CCSGG 3 cut(s) 182, 398, 521
NdeI CATATG 1 cut(s) 16
NdeII GATC 3 cut(s) 484, 664, 739
NlaIII CATG 2 cut(s) 627, 841
NlaIV GGNNCC 3 cut(s) 169, 575, 766
NsbI TGCGCA 1 cut(s) 441
NsiI ATGCAT 1 cut(s) 223
NspI RCATGY 1 cut(s) 627
PceI AGGCCT 1 cut(s) 471
PcsI WCGNNNNNNNCGW 2 cut(s) 201, 243
PfeI GAWTC 1 cut(s) 476
PinAI ACCGGT 1 cut(s) 789
PkrI GCNGC 3 cut(s) 25, 28, 638
PluTI GGCGCC 1 cut(s) 171
Psp6I CCWGG 2 cut(s) 280, 588
PspGI CCWGG 2 cut(s) 280, 588
PspN4I GGNNCC 3 cut(s) 169, 575, 766
PspPI GGNCC 2 cut(s) 347, 787
PstNI CAGNNNCTG 1 cut(s) 748
PsuI RGATCY 1 cut(s) 484
RsaI GTAC 4 cut(s) 406, 554, 568, 649
RsaNI GTAC 4 cut(s) 405, 553, 567, 648
SaqAI TTAA 1 cut(s) 909
SatI GCNGC 3 cut(s) 24, 27, 637
Sau3AI GATC 3 cut(s) 484, 664, 739
Sau96I GGNCC 2 cut(s) 347, 787
ScrFI CCNGG 5 cut(s) 182, 282, 398, 521, 590
SduI GDGCHC 3 cut(s) 104, 508, 526
SfaNI GCATC 4 cut(s) 208, 229, 230, 454
SfoI GGCGCC 1 cut(s) 169
SinI GGWCC 2 cut(s) 347, 787
SpeI ACTAGT 1 cut(s) 773
Sse9I AATT 5 cut(s) 30, 365, 444, 556, 885
SseBI AGGCCT 1 cut(s) 471
SsiI CCGC 2 cut(s) 547, 636
SspDI GGCGCC 1 cut(s) 167
SspMI CTAG 1 cut(s) 774
StuI AGGCCT 1 cut(s) 471
StyD4I CCNGG 5 cut(s) 180, 280, 396, 519, 588
TaaI ACNGT 2 cut(s) 257, 528
TaiI ACGT 1 cut(s) 101
TaqI TCGA 4 cut(s) 113, 234, 687, 755
TasI AATT 5 cut(s) 30, 365, 444, 556, 885
TatI WGTACW 1 cut(s) 552
TauI GCSGC 1 cut(s) 639
TfiI GAWTC 1 cut(s) 476
Tru1I TTAA 1 cut(s) 909
Tru9I TTAA 1 cut(s) 909
TscAI CASTG 6 cut(s) 262, 466, 533, 568, 766, 880
TseI GCWGC 2 cut(s) 23, 26
TspRI CASTG 6 cut(s) 262, 466, 533, 568, 766, 880
VpaK11BI GGWCC 2 cut(s) 347, 787
XceI RCATGY 1 cut(s) 627
XcmI CCANNNNNNNNNTGG 1 cut(s) 844
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 1 cut(s) 774
Zsp2I ATGCAT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.