MD11G1015300.v1.1

Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003999
Physical Location & Seq
Reverse (-)
1212949 .. 1214635
1687 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1015300.v1.1.491

Sequence Viewer

Length: 975 bp
ATGTCTTCATTTTCAATTGCAGGAGCAATATTTGTTCTTGTTATTACTAGTGTGTTGGGAAATGTTAACGCTCAACTAAGCTCAACCTTCTACAGTACCACATGCCCGAATGTGACGAGCATTGTTCGCGGTGTGGTCGAGCAAGCTCAACAGAATGACATCCGGATTGGTGCCAAACTCATCCGGGTGCATTTCCACGACTGCTTCGTCAATGGTTGTGATGGATCGATTATGCTAGACAACGCAGATGGAATAGATAGCGAGAAAGATGCAGGTCCCAATCTATCGACAGAAGGATACGACGTCGTAGATGATATCAAAACTGCTCTCGAGAATGTTTGCCCTGGTGTTGTCTCCTGTTCTGACATTTTAGCAATTGCTTCTCAAATTCTTGTTTCTGAGAATGGAGGGCCGACGTGGGAAGTTCAATTGGGAAGAAGAGACAGTAGGACAGCCAACCGAGCTGGTACCACTGCCATTCCAGGCTTTAACGAAAGCCTTGATCAACTTTCACAAAAATTTCGCGATGCAGGATTAGATTCCACTGATCTGGTTGCTTTATCAGGTGCTCATACATTTGGCCGGGCAAGATGTTCAACTTTCGTTCACCGCCTCTACAATTTCAGCGGCACCGGCAACCCGGACCCGACCATAGAAGCCGGATACTTGGAAACCCTCCGCCAAACATGTCCTCAAAACGGCAACGGAGATACATTGAACGACCTTGACCAATCCACCCGCGATGCCTTTGACAACAACTACTTTACAAATCTCCAAAACAACCGTGGGCTTCTCCAGACCGATCAGGTGCTGTTCTCAACTAGCGGAGACACCGTTGCGATTGTGAACCGCTTTGCAAATAGCCAAAGTGACTTTTTTGATAGCTTTGGTCAGTCCATGATCAATATGGGGAATATAGGAGTTTTGACAGGCACCAATGGAGAGATTAGGACCAATTGCAGAAGGGTTAATTAA

Protein Analysis

325

Amino Acids

34.87

Weight (kDa)

4.59

Isoelectric Point (pI)

30.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 42 - 288 9e-85 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 206
AatII GACGTC 1 cut(s) 306
Acc36I ACCTGC 1 cut(s) 263
Acc65I GGTACC 1 cut(s) 467
AccB1I GGYRCC 4 cut(s) 170, 467, 629, 932
AccII CGCG 3 cut(s) 129, 525, 741
AccIII TCCGGA 1 cut(s) 162
AciI CCGC 7 cut(s) 129, 610, 627, 679, 739, 825, 850
AclWI GGATC 1 cut(s) 232
AcoI YGGCCR 1 cut(s) 580
AcsI RAATTY 2 cut(s) 387, 518
AcyI GRCGYC 1 cut(s) 303
AfaI GTAC 2 cut(s) 97, 469
AfiI CCNNNNNNNGG 1 cut(s) 698
AflIII ACRYGT 1 cut(s) 686
AgsI TTSAA 4 cut(s) 15, 428, 597, 718
AhlI ACTAGT 1 cut(s) 47
AjiI CACGTC 1 cut(s) 417
AjnI CCWGG 2 cut(s) 343, 481
AluBI AGCT 4 cut(s) 81, 146, 464, 885
AluI AGCT 4 cut(s) 81, 146, 464, 885
Alw21I GWGCWC 1 cut(s) 571
Alw26I GTCTC 3 cut(s) 358, 435, 822
AlwI GGATC 1 cut(s) 232
AlwNI CAGNNNCTG 1 cut(s) 811
Ama87I CYCGRG 1 cut(s) 329
Aor13HI TCCGGA 1 cut(s) 162
AoxI GGCC 2 cut(s) 410, 580
ApoI RAATTY 2 cut(s) 387, 518
Asp718I GGTACC 1 cut(s) 467
AspS9I GGNCC 4 cut(s) 275, 410, 643, 951
AsuC2I CCSGG 3 cut(s) 185, 584, 641
AsuHPI GGTGA 1 cut(s) 599
AvaI CYCGRG 1 cut(s) 329
AvaII GGWCC 3 cut(s) 275, 643, 951
BanI GGYRCC 4 cut(s) 170, 467, 629, 932
Bbv12I GWGCWC 1 cut(s) 571
BccI CCATC 2 cut(s) 215, 242
BceAI ACGGC 1 cut(s) 715
BcgI CGANNNNNNTGC 2 cut(s) 251, 285
BciT130I CCWGG 2 cut(s) 345, 483
BciVI GTATCC 2 cut(s) 290, 656
BclI TGATCA 2 cut(s) 502, 900
BcnI CCSGG 3 cut(s) 185, 584, 641
BcoDI GTCTC 3 cut(s) 358, 435, 822
BcuI ACTAGT 1 cut(s) 47
BfaI CTAG 3 cut(s) 48, 236, 822
BfmI CTRYAG 1 cut(s) 91
BfuAI ACCTGC 1 cut(s) 263
BfuI GTATCC 2 cut(s) 290, 656
BisI GCNGC 1 cut(s) 628
BlsI GCNGC 1 cut(s) 629
Bme1390I CCNGG 5 cut(s) 185, 345, 483, 584, 641
Bme18I GGWCC 3 cut(s) 275, 643, 951
BmeT110I CYCGRG 1 cut(s) 329
BmgBI CACGTC 1 cut(s) 417
BmgT120I GGNCC 4 cut(s) 275, 410, 643, 951
BmiI GGNNCC 6 cut(s) 172, 277, 469, 631, 645, 934
BmrFI CCNGG 5 cut(s) 185, 345, 483, 584, 641
BmsI GCATC 3 cut(s) 259, 517, 733
BpmI CTGGAG 1 cut(s) 779
BpuMI CCSGG 3 cut(s) 185, 584, 641
Bsa29I ATCGAT 1 cut(s) 227
BsaHI GRCGYC 1 cut(s) 303
BsaJI CCNNGG 2 cut(s) 343, 784
BsaWI WCCGGW 1 cut(s) 162
BsaXI ACNNNNNCTCC 2 cut(s) 819, 849
Bsc4I CCNNNNNNNGG 1 cut(s) 698
Bse118I RCCGGY 1 cut(s) 632
BseAI TCCGGA 1 cut(s) 162
BseBI CCWGG 2 cut(s) 345, 483
BseCI ATCGAT 1 cut(s) 227
BseDI CCNNGG 2 cut(s) 343, 784
BseGI GGATG 2 cut(s) 159, 180
BseLI CCNNNNNNNGG 1 cut(s) 698
BseMII CTCAG 1 cut(s) 390
Bsh1236I CGCG 3 cut(s) 129, 525, 741
BshFI GGCC 2 cut(s) 412, 582
BshNI GGYRCC 4 cut(s) 170, 467, 629, 932
BshVI ATCGAT 1 cut(s) 227
BsiHKAI GWGCWC 1 cut(s) 571
BsiHKCI CYCGRG 1 cut(s) 329
BsiSI CCGG 6 cut(s) 163, 184, 583, 633, 641, 660
BslFI GGGAC 1 cut(s) 261
BslI CCNNNNNNNGG 1 cut(s) 698
BsmAI GTCTC 3 cut(s) 358, 435, 822
BsmFI GGGAC 1 cut(s) 261
BsnI GGCC 2 cut(s) 412, 582
BsoBI CYCGRG 1 cut(s) 329
Bsp1286I GDGCHC 1 cut(s) 571
Bsp13I TCCGGA 1 cut(s) 162
Bsp143I GATC 5 cut(s) 224, 502, 547, 802, 900
Bsp68I TCGCGA 1 cut(s) 525
BspACI CCGC 7 cut(s) 129, 610, 627, 679, 739, 825, 850
BspANI GGCC 2 cut(s) 412, 582
BspCNI CTCAG 1 cut(s) 391
BspDI ATCGAT 1 cut(s) 227
BspEI TCCGGA 1 cut(s) 162
BspFNI CGCG 3 cut(s) 129, 525, 741
BspLI GGNNCC 6 cut(s) 172, 277, 469, 631, 645, 934
BspMI ACCTGC 1 cut(s) 263
BspPI GGATC 1 cut(s) 232
BspT107I GGYRCC 4 cut(s) 170, 467, 629, 932
BsrFI RCCGGY 1 cut(s) 632
BssAI RCCGGY 1 cut(s) 632
BssECI CCNNGG 2 cut(s) 343, 784
BssMI GATC 5 cut(s) 224, 502, 547, 802, 900
BssNI GRCGYC 1 cut(s) 303
Bst2UI CCWGG 2 cut(s) 345, 483
Bst4CI ACNGT 4 cut(s) 95, 446, 785, 835
Bst6I CTCTTC 1 cut(s) 433
BstACI GRCGYC 1 cut(s) 303
BstC8I GCNNGC 1 cut(s) 144
BstDEI CTNAG 2 cut(s) 77, 399
BstDSI CCRYGG 1 cut(s) 784
BstF5I GGATG 2 cut(s) 159, 180
BstFNI CGCG 3 cut(s) 129, 525, 741
BstKTI GATC 5 cut(s) 227, 505, 550, 805, 903
BstMAI GTCTC 3 cut(s) 358, 435, 822
BstMBI GATC 5 cut(s) 224, 502, 547, 802, 900
BstMWI GCNNNNNNNGC 3 cut(s) 126, 461, 633
BstNI CCWGG 2 cut(s) 345, 483
BstNSI RCATGY 2 cut(s) 105, 690
BstSCI CCNGG 5 cut(s) 183, 343, 481, 582, 639
BstSFI CTRYAG 1 cut(s) 91
BstUI CGCG 3 cut(s) 129, 525, 741
BstXI CCANNNNNNTGG 1 cut(s) 550
Bsu15I ATCGAT 1 cut(s) 227
BsuI GTATCC 2 cut(s) 290, 656
BsuRI GGCC 2 cut(s) 412, 582
BsuTUI ATCGAT 1 cut(s) 227
BtgI CCRYGG 1 cut(s) 784
BtgZI GCGATG 2 cut(s) 540, 756
BtrI CACGTC 1 cut(s) 417
BtsCI GGATG 2 cut(s) 159, 180
BtsI GCAGTG 1 cut(s) 471
BtsIMutI CAGTG 2 cut(s) 471, 543
BtuMI TCGCGA 1 cut(s) 525
BveI ACCTGC 1 cut(s) 263
Cac8I GCNNGC 1 cut(s) 144
CaiI CAGNNNCTG 1 cut(s) 811
Cfr10I RCCGGY 1 cut(s) 632
Cfr13I GGNCC 4 cut(s) 275, 410, 643, 951
ClaI ATCGAT 1 cut(s) 227
Csp6I GTAC 2 cut(s) 96, 468
CviAII CATG 3 cut(s) 102, 687, 898
CviQI GTAC 2 cut(s) 96, 468
DdeI CTNAG 2 cut(s) 77, 399
DpnI GATC 5 cut(s) 226, 504, 549, 804, 902
DpnII GATC 5 cut(s) 224, 502, 547, 802, 900
DrdI GACNNNNNNGTC 1 cut(s) 206
DseDI GACNNNNNNGTC 1 cut(s) 206
EaeI YGGCCR 1 cut(s) 580
Eam1104I CTCTTC 1 cut(s) 433
EarI CTCTTC 1 cut(s) 433
EciI GGCGGA 1 cut(s) 668
Eco32I GATATC 1 cut(s) 316
Eco47I GGWCC 3 cut(s) 275, 643, 951
Eco88I CYCGRG 1 cut(s) 329
EcoO109I RGGNCCY 1 cut(s) 275
EcoRII CCWGG 2 cut(s) 343, 481
EcoRV GATATC 1 cut(s) 316
FaeI CATG 3 cut(s) 105, 690, 901
FaiI YATR 8 cut(s) 103, 233, 573, 653, 688, 899, 908, 917
FaqI GGGAC 1 cut(s) 261
FatI CATG 3 cut(s) 101, 686, 897
FauI CCCGC 1 cut(s) 746
FbaI TGATCA 2 cut(s) 502, 900
Fnu4HI GCNGC 1 cut(s) 628
FokI GGATG 2 cut(s) 146, 167
Fsp4HI GCNGC 1 cut(s) 628
FspBI CTAG 3 cut(s) 48, 236, 822
GluI GCNGC 1 cut(s) 628
GsuI CTGGAG 1 cut(s) 779
HaeIII GGCC 2 cut(s) 412, 582
HapII CCGG 6 cut(s) 163, 184, 583, 633, 641, 660
Hin1I GRCGYC 1 cut(s) 303
Hin1II CATG 3 cut(s) 105, 690, 901
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
HinfI GANTC 1 cut(s) 539
HpaI GTTAAC 1 cut(s) 67
HpaII CCGG 6 cut(s) 163, 184, 583, 633, 641, 660
HphI GGTGA 1 cut(s) 599
Hpy166II GTNNAC 3 cut(s) 67, 607, 847
Hpy188I TCNGA 2 cut(s) 364, 400
Hpy188III TCNNGA 5 cut(s) 163, 329, 331, 524, 796
Hpy8I GTNNAC 3 cut(s) 67, 607, 847
Hpy99I CGWCG 3 cut(s) 305, 308, 418
HpyAV CCTTC 3 cut(s) 97, 287, 957
HpyCH4III ACNGT 4 cut(s) 95, 446, 785, 835
HpyCH4IV ACGT 2 cut(s) 303, 416
HpyCH4V TGCA 6 cut(s) 20, 190, 272, 530, 857, 960
HpyF10VI GCNNNNNNNGC 3 cut(s) 126, 461, 633
HpyF3I CTNAG 2 cut(s) 77, 399
HpySE526I ACGT 2 cut(s) 303, 416
Hsp92I GRCGYC 1 cut(s) 303
Hsp92II CATG 3 cut(s) 105, 690, 901
Kpn2I TCCGGA 1 cut(s) 162
KpnI GGTACC 1 cut(s) 471
Ksp22I TGATCA 2 cut(s) 502, 900
KspAI GTTAAC 1 cut(s) 67
Kzo9I GATC 5 cut(s) 224, 502, 547, 802, 900
LmnI GCTCC 1 cut(s) 23
LweI GCATC 3 cut(s) 259, 517, 733
MaeI CTAG 3 cut(s) 48, 236, 822
MaeII ACGT 2 cut(s) 303, 416
MaeIII GTNAC 2 cut(s) 112, 869
MalI GATC 5 cut(s) 226, 504, 549, 804, 902
MboI GATC 5 cut(s) 224, 502, 547, 802, 900
MboII GAAGA 2 cut(s) 447, 450
MfeI CAATTG 4 cut(s) 15, 375, 428, 955
MhlI GDGCHC 1 cut(s) 571
MluCI AATT 8 cut(s) 15, 375, 387, 428, 518, 619, 955, 970
MnlI CCTC 4 cut(s) 401, 623, 686, 702
MroI TCCGGA 1 cut(s) 162
MseI TTAA 4 cut(s) 66, 489, 969, 973
MslI CAYNNNNRTG 1 cut(s) 185
MspA1I CMGCKG 1 cut(s) 627
MspI CCGG 6 cut(s) 163, 184, 583, 633, 641, 660
MspR9I CCNGG 5 cut(s) 185, 345, 483, 584, 641
MunI CAATTG 4 cut(s) 15, 375, 428, 955
MvaI CCWGG 2 cut(s) 345, 483
MvnI CGCG 3 cut(s) 129, 525, 741
MwoI GCNNNNNNNGC 3 cut(s) 126, 461, 633
NciI CCSGG 3 cut(s) 185, 584, 641
NdeII GATC 5 cut(s) 224, 502, 547, 802, 900
NlaIII CATG 3 cut(s) 105, 690, 901
NlaIV GGNNCC 6 cut(s) 172, 277, 469, 631, 645, 934
NmuCI GTSAC 2 cut(s) 112, 869
NruI TCGCGA 1 cut(s) 525
NspI RCATGY 2 cut(s) 105, 690
PacI TTAATTAA 1 cut(s) 973
PaeR7I CTCGAG 1 cut(s) 329
PciI ACATGT 1 cut(s) 686
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PfeI GAWTC 1 cut(s) 539
PkrI GCNGC 1 cut(s) 629
PpuMI RGGWCCY 1 cut(s) 275
PscI ACATGT 1 cut(s) 686
Psp5II RGGWCCY 1 cut(s) 275
Psp6I CCWGG 2 cut(s) 343, 481
PspGI CCWGG 2 cut(s) 343, 481
PspN4I GGNNCC 6 cut(s) 172, 277, 469, 631, 645, 934
PspPI GGNCC 4 cut(s) 275, 410, 643, 951
PspPPI RGGWCCY 1 cut(s) 275
PstNI CAGNNNCTG 1 cut(s) 811
RruI TCGCGA 1 cut(s) 525
RsaI GTAC 2 cut(s) 97, 469
RsaNI GTAC 2 cut(s) 96, 468
RseI CAYNNNNRTG 1 cut(s) 185
SaqAI TTAA 4 cut(s) 66, 489, 969, 973
SatI GCNGC 1 cut(s) 628
Sau3AI GATC 5 cut(s) 224, 502, 547, 802, 900
Sau96I GGNCC 4 cut(s) 275, 410, 643, 951
ScrFI CCNGG 5 cut(s) 185, 345, 483, 584, 641
SduI GDGCHC 1 cut(s) 571
SfaNI GCATC 3 cut(s) 259, 517, 733
SfcI CTRYAG 1 cut(s) 91
Sfr274I CTCGAG 1 cut(s) 329
SinI GGWCC 3 cut(s) 275, 643, 951
SlaI CTCGAG 1 cut(s) 329
SmiMI CAYNNNNRTG 1 cut(s) 185
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
SpeI ACTAGT 1 cut(s) 47
Sse9I AATT 8 cut(s) 15, 375, 387, 428, 518, 619, 955, 970
SsiI CCGC 7 cut(s) 129, 610, 627, 679, 739, 825, 850
SspI AATATT 1 cut(s) 30
SspMI CTAG 3 cut(s) 48, 236, 822
StyD4I CCNGG 5 cut(s) 183, 343, 481, 582, 639
TaaI ACNGT 4 cut(s) 95, 446, 785, 835
TaiI ACGT 2 cut(s) 306, 419
TaqI TCGA 4 cut(s) 138, 227, 287, 330
TaqII GACCGA 1 cut(s) 815
TasI AATT 8 cut(s) 15, 375, 387, 428, 518, 619, 955, 970
TauI GCSGC 1 cut(s) 630
TfiI GAWTC 1 cut(s) 539
Tru1I TTAA 4 cut(s) 66, 489, 969, 973
Tru9I TTAA 4 cut(s) 66, 489, 969, 973
TscAI CASTG 2 cut(s) 478, 550
TseFI GTSAC 2 cut(s) 112, 869
Tsp45I GTSAC 2 cut(s) 112, 869
TspGWI ACGGA 1 cut(s) 720
TspRI CASTG 2 cut(s) 478, 550
VpaK11BI GGWCC 3 cut(s) 275, 643, 951
XapI RAATTY 2 cut(s) 387, 518
XceI RCATGY 2 cut(s) 105, 690
XcmI CCANNNNNNNNNTGG 2 cut(s) 782, 904
XhoI CTCGAG 1 cut(s) 329
XspI CTAG 3 cut(s) 48, 236, 822
ZraI GACGTC 1 cut(s) 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.