Rw5G049300

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0003999
Physical Location & Seq
Forward (+)
85461618 .. 85463611
1994 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G049300.1

Sequence Viewer

Length: 975 bp
ATGTCTATATTTTCATATGTTGCAGCAGCAATTGTTCTCTTTACCATTGTGCTGGGCAACTCCAATGCTCAGCTCAGCTCCACTTTTTACGAGAAAACGTGCCCTAACGTGTCGAGCGTGGTTCGTAATGTGGTGGAGCAAGCTCAACAGAATGATATTCGTATTGGCGCCAAACTCATCCGGGTTCACTTCCACGACTGCTTCGTCAATGGGTGTGATGCATCACTTTTGCTCGACGATGCAGACGACATAGACAGCGAAAAAAATGCAGCTCCGAATCTATCCACAGCTGGATATCCTGTGATTGATGACATCAAAACTGCACTTGAGAATGTTTGCCCTGGTGTTGTCTCCTGTGCTGATATTGTAGCTCTTGCTTCTCAGATACTCGTTTCTGAGAATGGAGGACCAACTTGGGAAGTTCAATTGGGAAGAAGAGACAGTAGGACAGCCAACCGGGCTGGTACTACTGCCATTCCAGGCCCTACAGAAACCCTTGCGGAAATTACCAAAAAGTTCACTGATGCAGGACTTGATTCTACAGATCTCGTTGCACTGTCAGGTGCTCATACATTTGGCCGGGCACAGTGTTCAACTTTCGTCCACCGCCTATACAATTTCAGTGGTACGGGGAACCCCGACCCGAGCCTGGACACATCTTACTTGGAAACCCTACGCAAAACATGTCCCCAAGGCGGCAATGGAGGTACATTGGCCAACCTTGATCCTTCTACGCCCAATGGCTTCGACAACAACTACTTCACAAACCTTCAGAACAAGCAGGGGCTTCTCCAGACGGATCAGGAGCTGTTTTCGACCAGTGGAGCCGACACTATTTCCATTGTGGACCGGTTTGCTAATAGCCAAAGCGACTTCTTTGACACTTTTGCTAAGTCCATGATCAATATGGGAAATATAAAACCTTTGATGGGCAGTGATGGAGAGATTAGGTCTGATTGTAAAAGAGTTAACTAA

Protein Analysis

324

Amino Acids

34.73

Weight (kDa)

4.67

Isoelectric Point (pI)

30.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 41 - 288 3.1e-85 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 203
AccB1I GGYRCC 1 cut(s) 167
AciI CCGC 3 cut(s) 500, 607, 696
AclWI GGATC 2 cut(s) 719, 807
AcoI YGGCCR 2 cut(s) 577, 714
AcuI CTGAAG 1 cut(s) 755
AcyI GRCGYC 1 cut(s) 168
AfaI GTAC 3 cut(s) 466, 628, 709
AfiI CCNNNNNNNGG 3 cut(s) 649, 695, 929
AflIII ACRYGT 2 cut(s) 108, 683
AgeI ACCGGT 1 cut(s) 849
AgsI TTSAA 2 cut(s) 425, 594
AjnI CCWGG 3 cut(s) 340, 478, 648
AluBI AGCT 7 cut(s) 73, 78, 143, 272, 290, 371, 808
AluI AGCT 7 cut(s) 73, 78, 143, 272, 290, 371, 808
Alw21I GWGCWC 1 cut(s) 568
Alw26I GTCTC 2 cut(s) 355, 432
AlwI GGATC 2 cut(s) 719, 807
AlwNI CAGNNNCTG 1 cut(s) 808
Ama87I CYCGRG 1 cut(s) 643
AoxI GGCC 3 cut(s) 481, 577, 714
ApeKI GCWGC 3 cut(s) 23, 26, 269
AsiGI ACCGGT 1 cut(s) 849
AspLEI GCGC 1 cut(s) 170
AspS9I GGNCC 3 cut(s) 407, 482, 847
AsuC2I CCSGG 3 cut(s) 182, 458, 581
AvaI CYCGRG 1 cut(s) 643
AvaII GGWCC 2 cut(s) 407, 847
BaeGI GKGCMC 2 cut(s) 104, 586
BalI TGGCCA 1 cut(s) 716
BanI GGYRCC 1 cut(s) 167
Bbv12I GWGCWC 1 cut(s) 568
BbvI GCAGC 3 cut(s) 35, 38, 281
BccI CCATC 2 cut(s) 922, 932
BcgI CGANNNNNNTGC 2 cut(s) 248, 282
BciT130I CCWGG 3 cut(s) 342, 480, 650
BclI TGATCA 1 cut(s) 900
BcnI CCSGG 3 cut(s) 182, 458, 581
BcoDI GTCTC 2 cut(s) 355, 432
BfmI CTRYAG 2 cut(s) 486, 540
BfoI RGCGCY 1 cut(s) 171
BglI GCCNNNNNGGC 1 cut(s) 458
BglII AGATCT 1 cut(s) 544
BisI GCNGC 4 cut(s) 24, 27, 270, 697
BlpI GCTNAGC 2 cut(s) 69, 74
BlsI GCNGC 4 cut(s) 25, 28, 271, 698
Bme1390I CCNGG 6 cut(s) 182, 342, 458, 480, 581, 650
Bme18I GGWCC 2 cut(s) 407, 847
BmeT110I CYCGRG 1 cut(s) 643
BmgT120I GGNCC 3 cut(s) 407, 482, 847
BmiI GGNNCC 3 cut(s) 169, 635, 826
BmrFI CCNGG 6 cut(s) 182, 342, 458, 480, 581, 650
BmsI GCATC 4 cut(s) 208, 229, 230, 514
BpmI CTGGAG 1 cut(s) 776
Bpu1102I GCTNAGC 2 cut(s) 69, 74
BpuEI CTTGAG 1 cut(s) 347
BpuMI CCSGG 3 cut(s) 182, 458, 581
BsaHI GRCGYC 1 cut(s) 168
BsaJI CCNNGG 2 cut(s) 340, 691
BsaWI WCCGGW 1 cut(s) 849
Bsc4I CCNNNNNNNGG 3 cut(s) 649, 695, 929
Bse118I RCCGGY 1 cut(s) 849
Bse1I ACTGG 1 cut(s) 819
Bse3DI GCAATG 1 cut(s) 706
BseBI CCWGG 3 cut(s) 342, 480, 650
BseDI CCNNGG 2 cut(s) 340, 691
BseGI GGATG 1 cut(s) 177
BseLI CCNNNNNNNGG 3 cut(s) 649, 695, 929
BseMI GCAATG 1 cut(s) 706
BseMII CTCAG 4 cut(s) 83, 88, 387, 395
BseNI ACTGG 1 cut(s) 819
BseSI GKGCMC 2 cut(s) 104, 586
BseXI GCAGC 3 cut(s) 35, 38, 281
BseYI CCCAGC 1 cut(s) 52
BsgI GTGCAG 1 cut(s) 306
BshFI GGCC 3 cut(s) 483, 579, 716
BshNI GGYRCC 1 cut(s) 167
BshTI ACCGGT 1 cut(s) 849
BsiHKAI GWGCWC 1 cut(s) 568
BsiHKCI CYCGRG 1 cut(s) 643
BsiSI CCGG 4 cut(s) 181, 457, 580, 850
BslFI GGGAC 1 cut(s) 672
BslI CCNNNNNNNGG 3 cut(s) 649, 695, 929
BsmAI GTCTC 2 cut(s) 355, 432
BsmFI GGGAC 1 cut(s) 672
BsnI GGCC 3 cut(s) 483, 579, 716
BsoBI CYCGRG 1 cut(s) 643
Bsp1286I GDGCHC 3 cut(s) 104, 568, 586
Bsp143I GATC 4 cut(s) 544, 724, 799, 900
Bsp1720I GCTNAGC 2 cut(s) 69, 74
BspACI CCGC 3 cut(s) 500, 607, 696
BspANI GGCC 3 cut(s) 483, 579, 716
BspCNI CTCAG 4 cut(s) 82, 87, 388, 394
BspLI GGNNCC 3 cut(s) 169, 635, 826
BspPI GGATC 2 cut(s) 719, 807
BspT107I GGYRCC 1 cut(s) 167
BsrDI GCAATG 1 cut(s) 706
BsrFI RCCGGY 1 cut(s) 849
BsrI ACTGG 1 cut(s) 819
BssAI RCCGGY 1 cut(s) 849
BssECI CCNNGG 2 cut(s) 340, 691
BssMI GATC 4 cut(s) 544, 724, 799, 900
BssNI GRCGYC 1 cut(s) 168
BssT1I CCWWGG 1 cut(s) 691
Bst2UI CCWGG 3 cut(s) 342, 480, 650
Bst4CI ACNGT 3 cut(s) 443, 558, 588
Bst6I CTCTTC 1 cut(s) 430
BstACI GRCGYC 1 cut(s) 168
BstC8I GCNNGC 1 cut(s) 141
BstDEI CTNAG 5 cut(s) 69, 74, 381, 396, 891
BstF5I GGATG 1 cut(s) 177
BstH2I RGCGCY 1 cut(s) 171
BstHHI GCGC 1 cut(s) 170
BstKTI GATC 4 cut(s) 547, 727, 802, 903
BstMAI GTCTC 2 cut(s) 355, 432
BstMBI GATC 4 cut(s) 544, 724, 799, 900
BstMWI GCNNNNNNNGC 1 cut(s) 458
BstNI CCWGG 3 cut(s) 342, 480, 650
BstNSI RCATGY 1 cut(s) 687
BstSCI CCNGG 6 cut(s) 180, 340, 456, 478, 579, 648
BstSFI CTRYAG 2 cut(s) 486, 540
BstSLI GKGCMC 2 cut(s) 104, 586
BstV1I GCAGC 3 cut(s) 35, 38, 281
BstX2I RGATCY 1 cut(s) 544
BstXI CCANNNNNNTGG 1 cut(s) 52
BstYI RGATCY 1 cut(s) 544
BsuRI GGCC 3 cut(s) 483, 579, 716
BtsCI GGATG 1 cut(s) 177
BtsI GCAGTG 1 cut(s) 940
BtsIMutI CAGTG 6 cut(s) 519, 554, 593, 628, 826, 940
Cac8I GCNNGC 1 cut(s) 141
CaiI CAGNNNCTG 1 cut(s) 808
CfoI GCGC 1 cut(s) 170
Cfr10I RCCGGY 1 cut(s) 849
Cfr13I GGNCC 3 cut(s) 407, 482, 847
Csp6I GTAC 3 cut(s) 465, 627, 708
CspAI ACCGGT 1 cut(s) 849
CspCI CAANNNNNGTGG 2 cut(s) 604, 639
CviAII CATG 2 cut(s) 684, 898
CviQI GTAC 3 cut(s) 465, 627, 708
DdeI CTNAG 5 cut(s) 69, 74, 381, 396, 891
DinI GGCGCC 1 cut(s) 169
DpnI GATC 4 cut(s) 546, 726, 801, 902
DpnII GATC 4 cut(s) 544, 724, 799, 900
DrdI GACNNNNNNGTC 1 cut(s) 203
DseDI GACNNNNNNGTC 1 cut(s) 203
EaeI YGGCCR 2 cut(s) 577, 714
Eam1104I CTCTTC 1 cut(s) 430
EarI CTCTTC 1 cut(s) 430
Eco130I CCWWGG 1 cut(s) 691
Eco32I GATATC 1 cut(s) 296
Eco47I GGWCC 2 cut(s) 407, 847
Eco57I CTGAAG 1 cut(s) 755
Eco88I CYCGRG 1 cut(s) 643
EcoO109I RGGNCCY 1 cut(s) 482
EcoRII CCWGG 3 cut(s) 340, 478, 648
EcoRV GATATC 1 cut(s) 296
EcoT14I CCWWGG 1 cut(s) 691
EcoT22I ATGCAT 1 cut(s) 223
EgeI GGCGCC 1 cut(s) 169
EheI GGCGCC 1 cut(s) 169
ErhI CCWWGG 1 cut(s) 691
FaeI CATG 2 cut(s) 687, 901
FaqI GGGAC 1 cut(s) 672
FatI CATG 2 cut(s) 683, 897
FauNDI CATATG 1 cut(s) 16
FbaI TGATCA 1 cut(s) 900
Fnu4HI GCNGC 4 cut(s) 24, 27, 270, 697
FokI GGATG 1 cut(s) 164
Fsp4HI GCNGC 4 cut(s) 24, 27, 270, 697
GlaI GCGC 1 cut(s) 169
GluI GCNGC 4 cut(s) 24, 27, 270, 697
GsaI CCCAGC 1 cut(s) 56
GsuI CTGGAG 1 cut(s) 776
HaeII RGCGCY 1 cut(s) 171
HaeIII GGCC 3 cut(s) 483, 579, 716
HapII CCGG 4 cut(s) 181, 457, 580, 850
HhaI GCGC 1 cut(s) 170
Hin1I GRCGYC 1 cut(s) 168
Hin1II CATG 2 cut(s) 687, 901
Hin6I GCGC 1 cut(s) 168
HinP1I GCGC 1 cut(s) 168
HincII GTYRAC 1 cut(s) 970
HindII GTYRAC 1 cut(s) 970
HinfI GANTC 2 cut(s) 277, 536
HpaI GTTAAC 1 cut(s) 970
HpaII CCGG 4 cut(s) 181, 457, 580, 850
Hpy166II GTNNAC 5 cut(s) 187, 519, 604, 847, 970
Hpy188I TCNGA 5 cut(s) 276, 384, 397, 774, 955
Hpy188III TCNNGA 2 cut(s) 793, 803
Hpy8I GTNNAC 5 cut(s) 187, 519, 604, 847, 970
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 2 cut(s) 738, 779
HpyCH4III ACNGT 3 cut(s) 443, 558, 588
HpyCH4IV ACGT 2 cut(s) 98, 108
HpyCH4V TGCA 7 cut(s) 23, 221, 242, 269, 323, 527, 554
HpyF10VI GCNNNNNNNGC 1 cut(s) 458
HpyF3I CTNAG 5 cut(s) 69, 74, 381, 396, 891
HpySE526I ACGT 2 cut(s) 98, 108
Hsp92I GRCGYC 1 cut(s) 168
Hsp92II CATG 2 cut(s) 687, 901
HspAI GCGC 1 cut(s) 168
KasI GGCGCC 1 cut(s) 167
Ksp22I TGATCA 1 cut(s) 900
KspAI GTTAAC 1 cut(s) 970
Kzo9I GATC 4 cut(s) 544, 724, 799, 900
LmnI GCTCC 5 cut(s) 83, 136, 277, 805, 824
Lsp1109I GCAGC 3 cut(s) 35, 38, 281
LweI GCATC 4 cut(s) 208, 229, 230, 514
MaeII ACGT 2 cut(s) 98, 108
MalI GATC 4 cut(s) 546, 726, 801, 902
MboI GATC 4 cut(s) 544, 724, 799, 900
MboII GAAGA 2 cut(s) 444, 447
MfeI CAATTG 2 cut(s) 30, 425
MflI RGATCY 1 cut(s) 544
MhlI GDGCHC 3 cut(s) 104, 568, 586
MlsI TGGCCA 1 cut(s) 716
MluCI AATT 4 cut(s) 30, 425, 504, 616
MluNI TGGCCA 1 cut(s) 716
Mly113I GGCGCC 1 cut(s) 168
MnlI CCTC 2 cut(s) 398, 698
Mox20I TGGCCA 1 cut(s) 716
Mph1103I ATGCAT 1 cut(s) 223
MscI TGGCCA 1 cut(s) 716
MseI TTAA 1 cut(s) 969
Msp20I TGGCCA 1 cut(s) 716
MspA1I CMGCKG 1 cut(s) 290
MspI CCGG 4 cut(s) 181, 457, 580, 850
MspR9I CCNGG 6 cut(s) 182, 342, 458, 480, 581, 650
MunI CAATTG 2 cut(s) 30, 425
MvaI CCWGG 3 cut(s) 342, 480, 650
MwoI GCNNNNNNNGC 1 cut(s) 458
NarI GGCGCC 1 cut(s) 168
NciI CCSGG 3 cut(s) 182, 458, 581
NdeI CATATG 1 cut(s) 16
NdeII GATC 4 cut(s) 544, 724, 799, 900
NlaIII CATG 2 cut(s) 687, 901
NlaIV GGNNCC 3 cut(s) 169, 635, 826
NsiI ATGCAT 1 cut(s) 223
NspI RCATGY 1 cut(s) 687
PciI ACATGT 1 cut(s) 683
PcsI WCGNNNNNNNCGW 3 cut(s) 114, 201, 243
PfeI GAWTC 2 cut(s) 277, 536
PinAI ACCGGT 1 cut(s) 849
PkrI GCNGC 4 cut(s) 25, 28, 271, 698
PluTI GGCGCC 1 cut(s) 171
PscI ACATGT 1 cut(s) 683
Psp6I CCWGG 3 cut(s) 340, 478, 648
PspFI CCCAGC 1 cut(s) 52
PspGI CCWGG 3 cut(s) 340, 478, 648
PspN4I GGNNCC 3 cut(s) 169, 635, 826
PspPI GGNCC 3 cut(s) 407, 482, 847
PstNI CAGNNNCTG 1 cut(s) 808
PsuI RGATCY 1 cut(s) 544
PvuII CAGCTG 1 cut(s) 290
RsaI GTAC 3 cut(s) 466, 628, 709
RsaNI GTAC 3 cut(s) 465, 627, 708
SaqAI TTAA 1 cut(s) 969
SatI GCNGC 4 cut(s) 24, 27, 270, 697
Sau3AI GATC 4 cut(s) 544, 724, 799, 900
Sau96I GGNCC 3 cut(s) 407, 482, 847
ScrFI CCNGG 6 cut(s) 182, 342, 458, 480, 581, 650
SduI GDGCHC 3 cut(s) 104, 568, 586
SfaNI GCATC 4 cut(s) 208, 229, 230, 514
SfcI CTRYAG 2 cut(s) 486, 540
SfoI GGCGCC 1 cut(s) 169
SinI GGWCC 2 cut(s) 407, 847
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
Sse9I AATT 4 cut(s) 30, 425, 504, 616
SsiI CCGC 3 cut(s) 500, 607, 696
SspDI GGCGCC 1 cut(s) 167
StyD4I CCNGG 6 cut(s) 180, 340, 456, 478, 579, 648
StyI CCWWGG 1 cut(s) 691
TaaI ACNGT 3 cut(s) 443, 558, 588
TaiI ACGT 2 cut(s) 101, 111
TaqI TCGA 4 cut(s) 113, 234, 747, 815
TasI AATT 4 cut(s) 30, 425, 504, 616
TauI GCSGC 1 cut(s) 699
TfiI GAWTC 2 cut(s) 277, 536
Tru1I TTAA 1 cut(s) 969
Tru9I TTAA 1 cut(s) 969
TscAI CASTG 6 cut(s) 526, 561, 593, 628, 826, 940
TseI GCWGC 3 cut(s) 23, 26, 269
TspGWI ACGGA 1 cut(s) 812
TspRI CASTG 6 cut(s) 526, 561, 593, 628, 826, 940
VpaK11BI GGWCC 2 cut(s) 407, 847
XceI RCATGY 1 cut(s) 687
XcmI CCANNNNNNNNNTGG 2 cut(s) 698, 904
Zsp2I ATGCAT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.