Rw5G048720

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0003999
Physical Location & Seq
Forward (+)
84652764 .. 84669881
17118 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G048720.1

Sequence Viewer

Length: 888 bp
ATGTCTACATTTTCATTTGTTGCAGCAGCAATTGTTCTCTTTACCATTGTGTTGGGCAATTCCAATGCTCAGCTCAGCTCCACTTTTTACGACACAACGTGCCCTAATGTGTCGAGCGTGGTTCGTAATATGGTGGAGCAAGCTCAACAGAATGATATTCGTATTGGCGCCAAACTCATCCGGGTTCACTTCCACGACTGCTTCGTCAATGGCTGTGATGCATCACTTTTGCTTGACAACGCAGACGGCGTAGAAAGCGAAAAAAATGCAGGTCCGAATCTATCCACAAATGTTTGCCCGGGTGTTGTCTCCTGTGTTGAAATTGTAGCTCTTGCTTCTCAGATACTCGTTTCTGAGAATGGAGGACCAACTTGGGAAGTTCAATTGGGAAGAAGAGACAGTAGGACAGCCAACCGGGCTGGTACTACTTTCACTGATGCAGGACTTGATTCTACAGATCTCGTTGCTTTGTCAGGTGCTCATACATTTGGCTGGGCACAGTGTTCAACTTTCGTCCACCGCCTATACAATTTCAGTGGTACCGGGAACCCCGACCCAAGCCTGGACACAACTTACTTGGAAACCCTACGCCAAACATGCCCCGAAGGCGGCGATGGAGGTACATTGGCCAACCTTGATCCTTCTACGCCCAATGGCTTCGACAACAACTACTTCACAAACCTTCAGAACAAGCAGGGGCTTCTCCAGACGGATCAGGAGCTGTTTTCGACCAGTGGAGCCGACACTGTTTCCATTGTGGACCGGTTTGCTAATAGCCAAAGCGACTTCTTTGACACTTTTGCTAAGTCCCTGATCAATATGAGAAATATAAAACCTTTGACGGGCAGTGATGGAGAGATTAGGTCTGATTGTAAAAGAGTTAACTAA

Protein Analysis

295

Amino Acids

31.71

Weight (kDa)

4.59

Isoelectric Point (pI)

33.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 41 - 259 3.6e-60 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 203
Acc36I ACCTGC 1 cut(s) 260
Acc65I GGTACC 1 cut(s) 539
AccB1I GGYRCC 2 cut(s) 167, 539
AccI GTMKAC 1 cut(s) 5
AciI CCGC 2 cut(s) 520, 609
AclWI GGATC 2 cut(s) 632, 720
AcoI YGGCCR 1 cut(s) 627
AcuI CTGAAG 1 cut(s) 668
AcyI GRCGYC 1 cut(s) 168
AdeI CACNNNGTG 1 cut(s) 99
AfaI GTAC 3 cut(s) 424, 541, 622
AfiI CCNNNNNNNGG 3 cut(s) 562, 608, 842
AgeI ACCGGT 1 cut(s) 762
AgsI TTSAA 3 cut(s) 320, 383, 507
AjnI CCWGG 1 cut(s) 561
AluBI AGCT 5 cut(s) 73, 78, 143, 329, 721
AluI AGCT 5 cut(s) 73, 78, 143, 329, 721
Alw21I GWGCWC 1 cut(s) 481
Alw26I GTCTC 2 cut(s) 313, 390
AlwI GGATC 2 cut(s) 632, 720
AlwNI CAGNNNCTG 1 cut(s) 721
Ama87I CYCGRG 1 cut(s) 298
AoxI GGCC 1 cut(s) 627
ApeKI GCWGC 2 cut(s) 23, 26
AsiGI ACCGGT 1 cut(s) 762
Asp718I GGTACC 1 cut(s) 539
AspLEI GCGC 1 cut(s) 170
AspS9I GGNCC 3 cut(s) 272, 365, 760
AsuC2I CCSGG 5 cut(s) 182, 299, 300, 416, 544
AvaI CYCGRG 1 cut(s) 298
AvaII GGWCC 3 cut(s) 272, 365, 760
BaeGI GKGCMC 2 cut(s) 104, 499
BalI TGGCCA 1 cut(s) 629
BanI GGYRCC 2 cut(s) 167, 539
Bbv12I GWGCWC 1 cut(s) 481
BbvI GCAGC 2 cut(s) 35, 38
BccI CCATC 2 cut(s) 608, 845
BceAI ACGGC 1 cut(s) 262
BcgI CGANNNNNNTGC 2 cut(s) 248, 282
BciT130I CCWGG 1 cut(s) 563
BclI TGATCA 1 cut(s) 813
BcnI CCSGG 5 cut(s) 182, 299, 300, 416, 544
BcoDI GTCTC 2 cut(s) 313, 390
BfmI CTRYAG 1 cut(s) 453
BfoI RGCGCY 1 cut(s) 171
BfuAI ACCTGC 1 cut(s) 260
BglI GCCNNNNNGGC 2 cut(s) 416, 606
BglII AGATCT 1 cut(s) 457
BisI GCNGC 3 cut(s) 24, 27, 610
BlpI GCTNAGC 2 cut(s) 69, 74
BlsI GCNGC 3 cut(s) 25, 28, 611
Bme1390I CCNGG 6 cut(s) 182, 299, 300, 416, 544, 563
Bme18I GGWCC 3 cut(s) 272, 365, 760
BmeT110I CYCGRG 1 cut(s) 298
BmgT120I GGNCC 3 cut(s) 272, 365, 760
BmiI GGNNCC 4 cut(s) 169, 541, 548, 739
BmrFI CCNGG 6 cut(s) 182, 299, 300, 416, 544, 563
BmsI GCATC 3 cut(s) 208, 230, 427
BpmI CTGGAG 1 cut(s) 689
Bpu1102I GCTNAGC 2 cut(s) 69, 74
BpuMI CCSGG 5 cut(s) 182, 299, 300, 416, 544
BsaHI GRCGYC 1 cut(s) 168
BsaJI CCNNGG 1 cut(s) 298
BsaWI WCCGGW 1 cut(s) 762
Bsc4I CCNNNNNNNGG 3 cut(s) 562, 608, 842
Bse118I RCCGGY 1 cut(s) 762
Bse1I ACTGG 1 cut(s) 732
BseBI CCWGG 1 cut(s) 563
BseDI CCNNGG 1 cut(s) 298
BseGI GGATG 1 cut(s) 177
BseLI CCNNNNNNNGG 3 cut(s) 562, 608, 842
BseMII CTCAG 4 cut(s) 83, 88, 345, 353
BseNI ACTGG 1 cut(s) 732
BseSI GKGCMC 2 cut(s) 104, 499
BseXI GCAGC 2 cut(s) 35, 38
BseYI CCCAGC 1 cut(s) 492
BshFI GGCC 1 cut(s) 629
BshNI GGYRCC 2 cut(s) 167, 539
BshTI ACCGGT 1 cut(s) 762
BsiHKAI GWGCWC 1 cut(s) 481
BsiHKCI CYCGRG 1 cut(s) 298
BsiSI CCGG 5 cut(s) 181, 299, 415, 543, 763
BslFI GGGAC 1 cut(s) 793
BslI CCNNNNNNNGG 3 cut(s) 562, 608, 842
BsmAI GTCTC 2 cut(s) 313, 390
BsmFI GGGAC 1 cut(s) 793
BsnI GGCC 1 cut(s) 629
BsoBI CYCGRG 1 cut(s) 298
Bsp1286I GDGCHC 3 cut(s) 104, 481, 499
Bsp143I GATC 4 cut(s) 457, 637, 712, 813
Bsp1720I GCTNAGC 2 cut(s) 69, 74
BspACI CCGC 2 cut(s) 520, 609
BspANI GGCC 1 cut(s) 629
BspCNI CTCAG 4 cut(s) 82, 87, 346, 352
BspLI GGNNCC 4 cut(s) 169, 541, 548, 739
BspMI ACCTGC 1 cut(s) 260
BspPI GGATC 2 cut(s) 632, 720
BspT107I GGYRCC 2 cut(s) 167, 539
BsrFI RCCGGY 1 cut(s) 762
BsrI ACTGG 1 cut(s) 732
BssAI RCCGGY 1 cut(s) 762
BssECI CCNNGG 1 cut(s) 298
BssMI GATC 4 cut(s) 457, 637, 712, 813
BssNI GRCGYC 1 cut(s) 168
Bst2UI CCWGG 1 cut(s) 563
Bst4CI ACNGT 3 cut(s) 401, 501, 748
Bst6I CTCTTC 1 cut(s) 388
BstACI GRCGYC 1 cut(s) 168
BstC8I GCNNGC 1 cut(s) 141
BstDEI CTNAG 5 cut(s) 69, 74, 339, 354, 804
BstF5I GGATG 1 cut(s) 177
BstH2I RGCGCY 1 cut(s) 171
BstHHI GCGC 1 cut(s) 170
BstKTI GATC 4 cut(s) 460, 640, 715, 816
BstMAI GTCTC 2 cut(s) 313, 390
BstMBI GATC 4 cut(s) 457, 637, 712, 813
BstMWI GCNNNNNNNGC 4 cut(s) 255, 416, 597, 606
BstNI CCWGG 1 cut(s) 563
BstNSI RCATGY 1 cut(s) 600
BstSCI CCNGG 6 cut(s) 180, 297, 298, 414, 542, 561
BstSFI CTRYAG 1 cut(s) 453
BstSLI GKGCMC 2 cut(s) 104, 499
BstV1I GCAGC 2 cut(s) 35, 38
BstX2I RGATCY 1 cut(s) 457
BstXI CCANNNNNNTGG 1 cut(s) 52
BstYI RGATCY 1 cut(s) 457
BsuRI GGCC 1 cut(s) 629
BtgZI GCGATG 1 cut(s) 627
BtsCI GGATG 1 cut(s) 177
BtsI GCAGTG 1 cut(s) 853
BtsIMutI CAGTG 6 cut(s) 432, 506, 541, 739, 744, 853
BveI ACCTGC 1 cut(s) 260
Cac8I GCNNGC 1 cut(s) 141
CaiI CAGNNNCTG 1 cut(s) 721
CfoI GCGC 1 cut(s) 170
Cfr10I RCCGGY 1 cut(s) 762
Cfr13I GGNCC 3 cut(s) 272, 365, 760
Cfr9I CCCGGG 1 cut(s) 298
Csp6I GTAC 3 cut(s) 423, 540, 621
CspAI ACCGGT 1 cut(s) 762
CspCI CAANNNNNGTGG 2 cut(s) 517, 552
CviAII CATG 1 cut(s) 597
CviQI GTAC 3 cut(s) 423, 540, 621
DdeI CTNAG 5 cut(s) 69, 74, 339, 354, 804
DinI GGCGCC 1 cut(s) 169
DpnI GATC 4 cut(s) 459, 639, 714, 815
DpnII GATC 4 cut(s) 457, 637, 712, 813
DraIII CACNNNGTG 1 cut(s) 99
DrdI GACNNNNNNGTC 1 cut(s) 203
DseDI GACNNNNNNGTC 1 cut(s) 203
EaeI YGGCCR 1 cut(s) 627
Eam1104I CTCTTC 1 cut(s) 388
EarI CTCTTC 1 cut(s) 388
Eco47I GGWCC 3 cut(s) 272, 365, 760
Eco57I CTGAAG 1 cut(s) 668
Eco88I CYCGRG 1 cut(s) 298
EcoRII CCWGG 1 cut(s) 561
EcoT22I ATGCAT 1 cut(s) 223
EgeI GGCGCC 1 cut(s) 169
EheI GGCGCC 1 cut(s) 169
FaeI CATG 1 cut(s) 600
FaiI YATR 6 cut(s) 131, 483, 526, 598, 821, 830
FaqI GGGAC 1 cut(s) 793
FatI CATG 1 cut(s) 596
FbaI TGATCA 1 cut(s) 813
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 3 cut(s) 24, 27, 610
FokI GGATG 1 cut(s) 164
Fsp4HI GCNGC 3 cut(s) 24, 27, 610
GlaI GCGC 1 cut(s) 169
GluI GCNGC 3 cut(s) 24, 27, 610
GsaI CCCAGC 1 cut(s) 496
GsuI CTGGAG 1 cut(s) 689
HaeII RGCGCY 1 cut(s) 171
HaeIII GGCC 1 cut(s) 629
HapII CCGG 5 cut(s) 181, 299, 415, 543, 763
HhaI GCGC 1 cut(s) 170
Hin1I GRCGYC 1 cut(s) 168
Hin1II CATG 1 cut(s) 600
Hin6I GCGC 1 cut(s) 168
HinP1I GCGC 1 cut(s) 168
HincII GTYRAC 1 cut(s) 883
HindII GTYRAC 1 cut(s) 883
HinfI GANTC 2 cut(s) 277, 449
HpaI GTTAAC 1 cut(s) 883
HpaII CCGG 5 cut(s) 181, 299, 415, 543, 763
Hpy166II GTNNAC 5 cut(s) 6, 187, 517, 760, 883
Hpy188I TCNGA 5 cut(s) 276, 342, 355, 687, 868
Hpy188III TCNNGA 2 cut(s) 706, 716
Hpy8I GTNNAC 5 cut(s) 6, 187, 517, 760, 883
HpyAV CCTTC 3 cut(s) 599, 651, 692
HpyCH4III ACNGT 3 cut(s) 401, 501, 748
HpyCH4IV ACGT 1 cut(s) 98
HpyCH4V TGCA 4 cut(s) 23, 221, 269, 440
HpyF10VI GCNNNNNNNGC 4 cut(s) 255, 416, 597, 606
HpyF3I CTNAG 5 cut(s) 69, 74, 339, 354, 804
HpySE526I ACGT 1 cut(s) 98
Hsp92I GRCGYC 1 cut(s) 168
Hsp92II CATG 1 cut(s) 600
HspAI GCGC 1 cut(s) 168
KasI GGCGCC 1 cut(s) 167
KpnI GGTACC 1 cut(s) 543
Ksp22I TGATCA 1 cut(s) 813
KspAI GTTAAC 1 cut(s) 883
Kzo9I GATC 4 cut(s) 457, 637, 712, 813
LmnI GCTCC 4 cut(s) 83, 136, 718, 737
Lsp1109I GCAGC 2 cut(s) 35, 38
LweI GCATC 3 cut(s) 208, 230, 427
MaeII ACGT 1 cut(s) 98
MalI GATC 4 cut(s) 459, 639, 714, 815
MboI GATC 4 cut(s) 457, 637, 712, 813
MboII GAAGA 2 cut(s) 402, 405
MfeI CAATTG 2 cut(s) 30, 383
MflI RGATCY 1 cut(s) 457
MhlI GDGCHC 3 cut(s) 104, 481, 499
MlsI TGGCCA 1 cut(s) 629
MluCI AATT 5 cut(s) 30, 58, 321, 383, 529
MluNI TGGCCA 1 cut(s) 629
Mly113I GGCGCC 1 cut(s) 168
MnlI CCTC 2 cut(s) 356, 611
Mox20I TGGCCA 1 cut(s) 629
Mph1103I ATGCAT 1 cut(s) 223
MscI TGGCCA 1 cut(s) 629
MseI TTAA 1 cut(s) 882
Msp20I TGGCCA 1 cut(s) 629
MspI CCGG 5 cut(s) 181, 299, 415, 543, 763
MspR9I CCNGG 6 cut(s) 182, 299, 300, 416, 544, 563
MunI CAATTG 2 cut(s) 30, 383
MvaI CCWGG 1 cut(s) 563
MwoI GCNNNNNNNGC 4 cut(s) 255, 416, 597, 606
NarI GGCGCC 1 cut(s) 168
NciI CCSGG 5 cut(s) 182, 299, 300, 416, 544
NdeII GATC 4 cut(s) 457, 637, 712, 813
NlaIII CATG 1 cut(s) 600
NlaIV GGNNCC 4 cut(s) 169, 541, 548, 739
NsiI ATGCAT 1 cut(s) 223
NspI RCATGY 1 cut(s) 600
PcsI WCGNNNNNNNCGW 2 cut(s) 201, 246
PfeI GAWTC 2 cut(s) 277, 449
PinAI ACCGGT 1 cut(s) 762
PkrI GCNGC 3 cut(s) 25, 28, 611
PluTI GGCGCC 1 cut(s) 171
Psp6I CCWGG 1 cut(s) 561
PspFI CCCAGC 1 cut(s) 492
PspGI CCWGG 1 cut(s) 561
PspN4I GGNNCC 4 cut(s) 169, 541, 548, 739
PspPI GGNCC 3 cut(s) 272, 365, 760
PstNI CAGNNNCTG 1 cut(s) 721
PsuI RGATCY 1 cut(s) 457
RsaI GTAC 3 cut(s) 424, 541, 622
RsaNI GTAC 3 cut(s) 423, 540, 621
SaqAI TTAA 1 cut(s) 882
SatI GCNGC 3 cut(s) 24, 27, 610
Sau3AI GATC 4 cut(s) 457, 637, 712, 813
Sau96I GGNCC 3 cut(s) 272, 365, 760
ScrFI CCNGG 6 cut(s) 182, 299, 300, 416, 544, 563
SduI GDGCHC 3 cut(s) 104, 481, 499
SfaNI GCATC 3 cut(s) 208, 230, 427
SfcI CTRYAG 1 cut(s) 453
SfoI GGCGCC 1 cut(s) 169
SinI GGWCC 3 cut(s) 272, 365, 760
SmaI CCCGGG 1 cut(s) 300
Sse9I AATT 5 cut(s) 30, 58, 321, 383, 529
SsiI CCGC 2 cut(s) 520, 609
SspDI GGCGCC 1 cut(s) 167
StyD4I CCNGG 6 cut(s) 180, 297, 298, 414, 542, 561
TaaI ACNGT 3 cut(s) 401, 501, 748
TaiI ACGT 1 cut(s) 101
TaqI TCGA 3 cut(s) 113, 660, 728
TasI AATT 5 cut(s) 30, 58, 321, 383, 529
TauI GCSGC 1 cut(s) 612
TfiI GAWTC 2 cut(s) 277, 449
Tru1I TTAA 1 cut(s) 882
Tru9I TTAA 1 cut(s) 882
TscAI CASTG 6 cut(s) 439, 506, 541, 739, 751, 853
TseI GCWGC 2 cut(s) 23, 26
TspGWI ACGGA 1 cut(s) 725
TspMI CCCGGG 1 cut(s) 298
TspRI CASTG 6 cut(s) 439, 506, 541, 739, 751, 853
VpaK11BI GGWCC 3 cut(s) 272, 365, 760
XceI RCATGY 1 cut(s) 600
XmaI CCCGGG 1 cut(s) 298
XmiI GTMKAC 1 cut(s) 5
Zsp2I ATGCAT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.