Rroxscaffold_1G00002590

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0003999
Physical Location & Seq
Reverse (-)
3045825 .. 3054408
8584 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00002590.1

Sequence Viewer

Length: 1287 bp
ATGGCCTCCATCGACGTTGTCACGGCGCCCACCGCCTTCTCTAGGATGCTTTCGGGGAGGTCCTTATCCCCTCTACATCATTGCTCGTCTCGACGGACATGCCATTCAGATCACTTCCATTCTACTTGTGAGGGATGGTGGATTGCGCGTTGCTTGTCGGCCTTGGCTACGTTGATTTATAGGCTTCTGAAAAAAACATTACTCGAAAAGAGTTTAAGGTTGAGGCGTGTAAACACTGCCCTTAAGAGTAAATTTCGCCCCTCGGAGACAACGCTTAAGTGTAGCAGGTTTGTAGCGCCCTATCCTCCAGGGTACAACAACCTCTATCCGTATAGGTCAGCAATTGTTCTCTTTACCCTTGTGTTGGGCAACTCCAATGCTCAGCTCAGCTCCACTTTTTACGACACAACGTGCCCTAATGTGTCGAGGGTGGTTCGTAATGTGGTGGAGAAAGCTCAACAGAATGATATTCGTATTGGCGCCAAACTCATCCGGGTTCACTTCCACGACTGCTTCGTCAATGGGTGTGATGCATCACTTTTGCTCGACGATGCAGACGACGTAGACAGCGAAAAAAATGCAACTCCGAATCTATCCACAGCTGGATATCCTGTGATTGATGACATCAAAACTGCACTCGAGAATGTTTGCCCTGGTGTTGTCTCCTGTGCTGATATTGTAGCTCTTGCTTCTCAGATACTCGTTTCTGAGAATGGAGGACCAACGTGGGAAGTTCAATTGGGAAGAAGAGACAGTAGGACAGCCAACCGGGCTGGTACTACTGCCATTCCAAGCCCTTTAGAAACCCTTGCGCAAATTACCAAAAAGTTCACTGATGCAGGACTTGATTCCACAGATCTCGTTGCTTTGTCAGGTGCTCATACATTTGGCCGGGCACAGTGTTCAACTTTCGTCCACCGCCTATACAATTTCAGTGGTACCGGGAACCCCGACCCAAGCCTGGACACAACTTACTTGGAAACCCTACGCAAAACATGTCCCGAAGGCGGCAATGGAGGTACATTGGCCAACCTTGATCCTTCTACACCCAATGGGTTCGACAACAACTACTTCACAAACCTTCAGAACAAGCAGGGGCTTCTCCAGACGGATCAGGAGCTGTTTTCGACCAGTGGAGCCGACACTATTTCCATTGTGGACCGGTTTGCTAATAGCCAAAGCGACTTCTTTGACACTTTTGCTAAGTCCATGATCAATATGGGAAATATAAAACCTTTGACGGGCAGTAATGGAGAGATTAGGTCTGATTGTAAAAGAGTTAACTAA

Protein Analysis

428

Amino Acids

46.74

Weight (kDa)

7.92

Isoelectric Point (pI)

35.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 145 - 392 2.3e-84 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 515
Acc16I TGCGCA 1 cut(s) 813
Acc36I ACCTGC 1 cut(s) 276
Acc65I GGTACC 1 cut(s) 938
AccB1I GGYRCC 3 cut(s) 25, 479, 938
AccI GTMKAC 1 cut(s) 564
AccII CGCG 1 cut(s) 148
AciI CCGC 3 cut(s) 33, 919, 1008
AclWI GGATC 2 cut(s) 1031, 1119
AcoI YGGCCR 2 cut(s) 889, 1026
AcsI RAATTY 1 cut(s) 251
AcuI CTGAAG 1 cut(s) 1067
AcyI GRCGYC 2 cut(s) 26, 480
AdeI CACNNNGTG 1 cut(s) 411
AfaI GTAC 4 cut(s) 314, 778, 940, 1021
AfiI CCNNNNNNNGG 5 cut(s) 42, 364, 961, 1007, 1241
AflII CTTAAG 2 cut(s) 242, 275
AflIII ACRYGT 1 cut(s) 995
AgeI ACCGGT 1 cut(s) 1161
AgsI TTSAA 2 cut(s) 737, 906
AjnI CCWGG 3 cut(s) 307, 652, 960
AluBI AGCT 6 cut(s) 385, 390, 455, 602, 683, 1120
AluI AGCT 6 cut(s) 385, 390, 455, 602, 683, 1120
Alw21I GWGCWC 1 cut(s) 880
Alw26I GTCTC 4 cut(s) 93, 260, 667, 744
AlwI GGATC 2 cut(s) 1031, 1119
AlwNI CAGNNNCTG 1 cut(s) 1120
Ama87I CYCGRG 1 cut(s) 638
AoxI GGCC 4 cut(s) 3, 159, 889, 1026
ApoI RAATTY 1 cut(s) 251
AsiGI ACCGGT 1 cut(s) 1161
Asp718I GGTACC 1 cut(s) 938
AspLEI GCGC 5 cut(s) 28, 148, 298, 482, 814
AspS9I GGNCC 3 cut(s) 60, 719, 1159
AsuC2I CCSGG 4 cut(s) 494, 770, 893, 943
AvaI CYCGRG 1 cut(s) 638
AvaII GGWCC 3 cut(s) 60, 719, 1159
BaeGI GKGCMC 2 cut(s) 416, 898
BalI TGGCCA 1 cut(s) 1028
BanI GGYRCC 3 cut(s) 25, 479, 938
Bbv12I GWGCWC 1 cut(s) 880
BccI CCATC 2 cut(s) 17, 129
BceAI ACGGC 1 cut(s) 39
BcgI CGANNNNNNTGC 4 cut(s) 81, 115, 560, 594
BciT130I CCWGG 3 cut(s) 309, 654, 962
BclI TGATCA 1 cut(s) 1212
BcnI CCSGG 4 cut(s) 494, 770, 893, 943
BcoDI GTCTC 4 cut(s) 93, 260, 667, 744
BfaI CTAG 1 cut(s) 42
BfoI RGCGCY 3 cut(s) 29, 299, 483
BfrI CTTAAG 2 cut(s) 242, 275
BfuAI ACCTGC 1 cut(s) 276
BglI GCCNNNNNGGC 1 cut(s) 770
BglII AGATCT 1 cut(s) 856
BisI GCNGC 1 cut(s) 1009
BlpI GCTNAGC 2 cut(s) 381, 386
BlsI GCNGC 1 cut(s) 1010
Bme1390I CCNGG 7 cut(s) 309, 494, 654, 770, 893, 943, 962
Bme18I GGWCC 3 cut(s) 60, 719, 1159
BmeT110I CYCGRG 1 cut(s) 638
BmgT120I GGNCC 3 cut(s) 60, 719, 1159
BmiI GGNNCC 5 cut(s) 27, 481, 940, 947, 1138
BmrFI CCNGG 7 cut(s) 309, 494, 654, 770, 893, 943, 962
BmsI GCATC 5 cut(s) 36, 520, 541, 542, 826
BpmI CTGGAG 2 cut(s) 291, 1088
Bpu1102I GCTNAGC 2 cut(s) 381, 386
BpuMI CCSGG 4 cut(s) 494, 770, 893, 943
BsaHI GRCGYC 2 cut(s) 26, 480
BsaJI CCNNGG 4 cut(s) 162, 261, 308, 652
BsaWI WCCGGW 1 cut(s) 1161
Bsc4I CCNNNNNNNGG 5 cut(s) 42, 364, 961, 1007, 1241
Bse118I RCCGGY 1 cut(s) 1161
Bse1I ACTGG 1 cut(s) 1131
Bse3DI GCAATG 2 cut(s) 79, 1018
BseBI CCWGG 3 cut(s) 309, 654, 962
BseDI CCNNGG 4 cut(s) 162, 261, 308, 652
BseGI GGATG 3 cut(s) 51, 140, 489
BseLI CCNNNNNNNGG 5 cut(s) 42, 364, 961, 1007, 1241
BseMI GCAATG 2 cut(s) 79, 1018
BseMII CTCAG 4 cut(s) 395, 400, 699, 707
BseNI ACTGG 1 cut(s) 1131
BseSI GKGCMC 2 cut(s) 416, 898
BsgI GTGCAG 1 cut(s) 618
Bsh1236I CGCG 1 cut(s) 148
BshFI GGCC 4 cut(s) 5, 161, 891, 1028
BshNI GGYRCC 3 cut(s) 25, 479, 938
BshTI ACCGGT 1 cut(s) 1161
BsiHKAI GWGCWC 1 cut(s) 880
BsiHKCI CYCGRG 1 cut(s) 638
BsiSI CCGG 5 cut(s) 493, 769, 892, 942, 1162
BslFI GGGAC 1 cut(s) 984
BslI CCNNNNNNNGG 5 cut(s) 42, 364, 961, 1007, 1241
BsmAI GTCTC 4 cut(s) 93, 260, 667, 744
BsmBI CGTCTC 1 cut(s) 93
BsmFI GGGAC 1 cut(s) 984
BsnI GGCC 4 cut(s) 5, 161, 891, 1028
BsoBI CYCGRG 1 cut(s) 638
Bsp1286I GDGCHC 3 cut(s) 416, 880, 898
Bsp143I GATC 5 cut(s) 109, 856, 1036, 1111, 1212
Bsp1720I GCTNAGC 2 cut(s) 381, 386
BspACI CCGC 3 cut(s) 33, 919, 1008
BspANI GGCC 4 cut(s) 5, 161, 891, 1028
BspCNI CTCAG 4 cut(s) 394, 399, 700, 706
BspFNI CGCG 1 cut(s) 148
BspLI GGNNCC 5 cut(s) 27, 481, 940, 947, 1138
BspMI ACCTGC 1 cut(s) 276
BspPI GGATC 2 cut(s) 1031, 1119
BspT107I GGYRCC 3 cut(s) 25, 479, 938
BspTI CTTAAG 2 cut(s) 242, 275
BsrDI GCAATG 2 cut(s) 79, 1018
BsrFI RCCGGY 1 cut(s) 1161
BsrI ACTGG 1 cut(s) 1131
BssAI RCCGGY 1 cut(s) 1161
BssECI CCNNGG 4 cut(s) 162, 261, 308, 652
BssMI GATC 5 cut(s) 109, 856, 1036, 1111, 1212
BssNI GRCGYC 2 cut(s) 26, 480
BssT1I CCWWGG 1 cut(s) 162
Bst2UI CCWGG 3 cut(s) 309, 654, 962
Bst4CI ACNGT 2 cut(s) 755, 900
Bst6I CTCTTC 1 cut(s) 742
BstACI GRCGYC 2 cut(s) 26, 480
BstAFI CTTAAG 2 cut(s) 242, 275
BstDEI CTNAG 5 cut(s) 381, 386, 693, 708, 1203
BstENI CCTNNNNNAGG 1 cut(s) 40
BstF5I GGATG 3 cut(s) 51, 140, 489
BstFNI CGCG 1 cut(s) 148
BstH2I RGCGCY 3 cut(s) 29, 299, 483
BstHHI GCGC 5 cut(s) 28, 148, 298, 482, 814
BstKTI GATC 5 cut(s) 112, 859, 1039, 1114, 1215
BstMAI GTCTC 4 cut(s) 93, 260, 667, 744
BstMBI GATC 5 cut(s) 109, 856, 1036, 1111, 1212
BstMWI GCNNNNNNNGC 2 cut(s) 32, 770
BstNI CCWGG 3 cut(s) 309, 654, 962
BstNSI RCATGY 2 cut(s) 102, 999
BstSCI CCNGG 7 cut(s) 307, 492, 652, 768, 891, 941, 960
BstSLI GKGCMC 2 cut(s) 416, 898
BstUI CGCG 1 cut(s) 148
BstX2I RGATCY 1 cut(s) 856
BstYI RGATCY 1 cut(s) 856
BsuRI GGCC 4 cut(s) 5, 161, 891, 1028
BtsCI GGATG 3 cut(s) 51, 140, 489
BtsI GCAGTG 1 cut(s) 234
BtsIMutI CAGTG 5 cut(s) 234, 831, 905, 940, 1138
BveI ACCTGC 1 cut(s) 276
CaiI CAGNNNCTG 1 cut(s) 1120
CfoI GCGC 5 cut(s) 28, 148, 298, 482, 814
Cfr10I RCCGGY 1 cut(s) 1161
Cfr13I GGNCC 3 cut(s) 60, 719, 1159
Csp6I GTAC 4 cut(s) 313, 777, 939, 1020
CspAI ACCGGT 1 cut(s) 1161
CspCI CAANNNNNGTGG 2 cut(s) 916, 951
CviAII CATG 3 cut(s) 99, 996, 1210
CviQI GTAC 4 cut(s) 313, 777, 939, 1020
DdeI CTNAG 5 cut(s) 381, 386, 693, 708, 1203
DinI GGCGCC 2 cut(s) 27, 481
DpnI GATC 5 cut(s) 111, 858, 1038, 1113, 1214
DpnII GATC 5 cut(s) 109, 856, 1036, 1111, 1212
DraIII CACNNNGTG 1 cut(s) 411
DrdI GACNNNNNNGTC 1 cut(s) 515
DseDI GACNNNNNNGTC 1 cut(s) 515
EaeI YGGCCR 2 cut(s) 889, 1026
Eam1104I CTCTTC 1 cut(s) 742
EarI CTCTTC 1 cut(s) 742
Eco130I CCWWGG 1 cut(s) 162
Eco32I GATATC 1 cut(s) 608
Eco47I GGWCC 3 cut(s) 60, 719, 1159
Eco57I CTGAAG 1 cut(s) 1067
Eco88I CYCGRG 1 cut(s) 638
EcoNI CCTNNNNNAGG 1 cut(s) 40
EcoO109I RGGNCCY 1 cut(s) 60
EcoRII CCWGG 3 cut(s) 307, 652, 960
EcoRV GATATC 1 cut(s) 608
EcoT14I CCWWGG 1 cut(s) 162
EcoT22I ATGCAT 1 cut(s) 535
EgeI GGCGCC 2 cut(s) 27, 481
EheI GGCGCC 2 cut(s) 27, 481
ErhI CCWWGG 1 cut(s) 162
Esp3I CGTCTC 1 cut(s) 93
FaeI CATG 3 cut(s) 102, 999, 1213
FaiI YATR 9 cut(s) 100, 180, 333, 882, 925, 997, 1211, 1220, 1229
FaqI GGGAC 1 cut(s) 984
FatI CATG 3 cut(s) 98, 995, 1209
FbaI TGATCA 1 cut(s) 1212
FblI GTMKAC 1 cut(s) 564
Fnu4HI GCNGC 1 cut(s) 1009
FokI GGATG 3 cut(s) 58, 147, 476
Fsp4HI GCNGC 1 cut(s) 1009
FspBI CTAG 1 cut(s) 42
FspI TGCGCA 1 cut(s) 813
GlaI GCGC 5 cut(s) 27, 147, 297, 481, 813
GluI GCNGC 1 cut(s) 1009
GsuI CTGGAG 2 cut(s) 291, 1088
HaeII RGCGCY 3 cut(s) 29, 299, 483
HaeIII GGCC 4 cut(s) 5, 161, 891, 1028
HapII CCGG 5 cut(s) 493, 769, 892, 942, 1162
HhaI GCGC 5 cut(s) 28, 148, 298, 482, 814
Hin1I GRCGYC 2 cut(s) 26, 480
Hin1II CATG 3 cut(s) 102, 999, 1213
Hin6I GCGC 5 cut(s) 26, 146, 296, 480, 812
HinP1I GCGC 5 cut(s) 26, 146, 296, 480, 812
HincII GTYRAC 1 cut(s) 1282
HindII GTYRAC 1 cut(s) 1282
HinfI GANTC 2 cut(s) 589, 848
HpaI GTTAAC 1 cut(s) 1282
HpaII CCGG 5 cut(s) 493, 769, 892, 942, 1162
Hpy166II GTNNAC 7 cut(s) 232, 499, 565, 831, 916, 1159, 1282
Hpy188I TCNGA 8 cut(s) 109, 189, 265, 588, 696, 709, 1086, 1267
Hpy188III TCNNGA 5 cut(s) 90, 640, 1001, 1105, 1115
Hpy8I GTNNAC 7 cut(s) 232, 499, 565, 831, 916, 1159, 1282
Hpy99I CGWCG 4 cut(s) 17, 96, 551, 563
HpyAV CCTTC 4 cut(s) 46, 998, 1050, 1091
HpyCH4III ACNGT 2 cut(s) 755, 900
HpyCH4IV ACGT 5 cut(s) 15, 170, 410, 561, 725
HpyCH4V TGCA 5 cut(s) 533, 554, 581, 635, 839
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 770
HpyF3I CTNAG 5 cut(s) 381, 386, 693, 708, 1203
HpySE526I ACGT 5 cut(s) 15, 170, 410, 561, 725
Hsp92I GRCGYC 2 cut(s) 26, 480
Hsp92II CATG 3 cut(s) 102, 999, 1213
HspAI GCGC 5 cut(s) 26, 146, 296, 480, 812
KasI GGCGCC 2 cut(s) 25, 479
KpnI GGTACC 1 cut(s) 942
Ksp22I TGATCA 1 cut(s) 1212
KspAI GTTAAC 1 cut(s) 1282
Kzo9I GATC 5 cut(s) 109, 856, 1036, 1111, 1212
LmnI GCTCC 3 cut(s) 395, 1117, 1136
LweI GCATC 5 cut(s) 36, 520, 541, 542, 826
MaeI CTAG 1 cut(s) 42
MaeII ACGT 5 cut(s) 15, 170, 410, 561, 725
MaeIII GTNAC 1 cut(s) 19
MalI GATC 5 cut(s) 111, 858, 1038, 1113, 1214
MboI GATC 5 cut(s) 109, 856, 1036, 1111, 1212
MboII GAAGA 2 cut(s) 756, 759
MfeI CAATTG 2 cut(s) 342, 737
MflI RGATCY 1 cut(s) 856
MhlI GDGCHC 3 cut(s) 416, 880, 898
MlsI TGGCCA 1 cut(s) 1028
MluCI AATT 5 cut(s) 251, 342, 737, 816, 928
MluNI TGGCCA 1 cut(s) 1028
Mly113I GGCGCC 2 cut(s) 26, 480
Mox20I TGGCCA 1 cut(s) 1028
Mph1103I ATGCAT 1 cut(s) 535
MscI TGGCCA 1 cut(s) 1028
MseI TTAA 4 cut(s) 215, 243, 276, 1281
Msp20I TGGCCA 1 cut(s) 1028
MspA1I CMGCKG 1 cut(s) 602
MspCI CTTAAG 2 cut(s) 242, 275
MspI CCGG 5 cut(s) 493, 769, 892, 942, 1162
MspR9I CCNGG 7 cut(s) 309, 494, 654, 770, 893, 943, 962
MunI CAATTG 2 cut(s) 342, 737
MvaI CCWGG 3 cut(s) 309, 654, 962
MvnI CGCG 1 cut(s) 148
MwoI GCNNNNNNNGC 2 cut(s) 32, 770
NarI GGCGCC 2 cut(s) 26, 480
NciI CCSGG 4 cut(s) 494, 770, 893, 943
NdeII GATC 5 cut(s) 109, 856, 1036, 1111, 1212
NlaIII CATG 3 cut(s) 102, 999, 1213
NlaIV GGNNCC 5 cut(s) 27, 481, 940, 947, 1138
NmuCI GTSAC 1 cut(s) 19
NsbI TGCGCA 1 cut(s) 813
NsiI ATGCAT 1 cut(s) 535
NspI RCATGY 2 cut(s) 102, 999
PaeR7I CTCGAG 1 cut(s) 638
PciI ACATGT 1 cut(s) 995
PcsI WCGNNNNNNNCGW 3 cut(s) 513, 555, 567
PfeI GAWTC 2 cut(s) 589, 848
PflFI GACNNNGTC 1 cut(s) 17
PinAI ACCGGT 1 cut(s) 1161
PkrI GCNGC 1 cut(s) 1010
PluTI GGCGCC 2 cut(s) 29, 483
PpuMI RGGWCCY 1 cut(s) 60
PscI ACATGT 1 cut(s) 995
Psp5II RGGWCCY 1 cut(s) 60
Psp6I CCWGG 3 cut(s) 307, 652, 960
PspGI CCWGG 3 cut(s) 307, 652, 960
PspN4I GGNNCC 5 cut(s) 27, 481, 940, 947, 1138
PspPI GGNCC 3 cut(s) 60, 719, 1159
PspPPI RGGWCCY 1 cut(s) 60
PstNI CAGNNNCTG 1 cut(s) 1120
PsuI RGATCY 1 cut(s) 856
PsyI GACNNNGTC 1 cut(s) 17
PvuII CAGCTG 1 cut(s) 602
RsaI GTAC 4 cut(s) 314, 778, 940, 1021
RsaNI GTAC 4 cut(s) 313, 777, 939, 1020
SaqAI TTAA 4 cut(s) 215, 243, 276, 1281
SatI GCNGC 1 cut(s) 1009
Sau3AI GATC 5 cut(s) 109, 856, 1036, 1111, 1212
Sau96I GGNCC 3 cut(s) 60, 719, 1159
ScrFI CCNGG 7 cut(s) 309, 494, 654, 770, 893, 943, 962
SduI GDGCHC 3 cut(s) 416, 880, 898
SfaNI GCATC 5 cut(s) 36, 520, 541, 542, 826
SfoI GGCGCC 2 cut(s) 27, 481
Sfr274I CTCGAG 1 cut(s) 638
SinI GGWCC 3 cut(s) 60, 719, 1159
SlaI CTCGAG 1 cut(s) 638
SmlI CTYRAG 3 cut(s) 242, 275, 638
SmoI CTYRAG 3 cut(s) 242, 275, 638
Sse9I AATT 5 cut(s) 251, 342, 737, 816, 928
SsiI CCGC 3 cut(s) 33, 919, 1008
SspDI GGCGCC 2 cut(s) 25, 479
SspMI CTAG 1 cut(s) 42
StyD4I CCNGG 7 cut(s) 307, 492, 652, 768, 891, 941, 960
StyI CCWWGG 1 cut(s) 162
TaaI ACNGT 2 cut(s) 755, 900
TaiI ACGT 5 cut(s) 18, 173, 413, 564, 728
TaqI TCGA 8 cut(s) 12, 91, 204, 425, 546, 639, 1059, 1127
TasI AATT 5 cut(s) 251, 342, 737, 816, 928
TauI GCSGC 1 cut(s) 1011
TfiI GAWTC 2 cut(s) 589, 848
Tru1I TTAA 4 cut(s) 215, 243, 276, 1281
Tru9I TTAA 4 cut(s) 215, 243, 276, 1281
TscAI CASTG 5 cut(s) 241, 838, 905, 940, 1138
TseFI GTSAC 1 cut(s) 19
Tsp45I GTSAC 1 cut(s) 19
TspGWI ACGGA 3 cut(s) 109, 318, 1124
TspRI CASTG 5 cut(s) 241, 838, 905, 940, 1138
Tth111I GACNNNGTC 1 cut(s) 17
Vha464I CTTAAG 2 cut(s) 242, 275
VpaK11BI GGWCC 3 cut(s) 60, 719, 1159
XagI CCTNNNNNAGG 1 cut(s) 40
XapI RAATTY 1 cut(s) 251
XceI RCATGY 2 cut(s) 102, 999
XcmI CCANNNNNNNNNTGG 1 cut(s) 1216
XhoI CTCGAG 1 cut(s) 638
XmiI GTMKAC 1 cut(s) 564
XspI CTAG 1 cut(s) 42
Zsp2I ATGCAT 1 cut(s) 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.