MD03G1054800.v1.1

Ferrous iron transport protein B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002736
Physical Location & Seq
Forward (+)
4368557 .. 4372899
4343 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1054800.v1.1.491

Sequence Viewer

Length: 1707 bp
ATGTCTGCAGAGGCTAAATTAAGGTTTAAGCTCAATAAGAAACTCAAAGCATCTATTGGAAGGCCCAAGTTTCACAGTGATGGAAAAAAGGATGATGATAATAGGAAGGGGAGCTTTAATAAGAAGGGGATAAAGTTTCATAATTCTGAGGAAGGGAGTCGTGGAGATCTTGATAAAAGGGACGATTTTAAAACCATGAATGGTGATAGGAGAAGAAAGAGAATCTATGGTGACCAAGACACAGATCGGGATTCAATGTCTTCTAGAAATGGAGAGGCATCTTATCGGAAGGCTTTTTCTAGGAAAGGACCGAACCAAGTAAGGGATGGTTTTGATGGTGAAAGGAACGATACTAGAGCTCCTTTGCGTCCTCGCTCTAGGTGGGGTTCTAATAGATTAGAGAATGCTGATTCTGAAACTAAACGTTTTTCTCCTAGGAGTCCCAAAGTCTCCAATAAAACCAAAAGTAAGGATGGTTTTGGAAATGAAAAGAACGCAAGTGAATCTCCTGGGCATCCTCGCTCTATCTGGGTTTCTAATGGATTAGAGAATGCTACTTCTGAAAATAAACGTTTTTCTTCTAAGGATAGAGGTGCAAAAGTTTCCAGTAAAACCAAATATAAGGAGGGCGATAAGATGGAATCTGTTAATACCGATGGTGGTCAGGCTAGTGCACTGAAGAAACATAACAAAGTTAAGCCGGATAAGAAAATCTTGAATGAAGAATCACGGGTGATAGATGATCAGCCAAAGAAGAGGAAGCGAATTCGATTAGATCCTAATGATATCTCAAATAAAAGATTTGATGATACTATTGTCATAAACGAAAGCAAAAAGGAGAAGGAAAAAGATGAGGAGGAAAAAGCTGAAATCTCAAAAAATGCACAGTTTCGTGCAATACAACCTAGTTCCTCAATCCTCTCTTATGTGGAAGATAATTTGTTAGGTCGTAGACGCTTGATAGAGTTGAGGAGGGCAGGCTACAACACTGAGCTTTCTGCCCCATTGGATAATATTCCTTCCTCTACCAGCTCAGAAAGAGAGAATATTGAGGAAAATATATTTAGAAATAAATTGACATTTTTTGCCGCTGCAAAAGTTTCATCATCATTTCCACCTCCTGATCTTCCAGAGATTGCATTTGCAGGGAGGTCAAATGTCGGGAAATCCTCACTACTTAATGCACTTACTAGACAATGGGGTGTTGTACGAACGTCAGACAAGCCTGGTCTTACTCAGACTATTAATTTCTTCAATCTAGGATCAAAGCTCTGCTTGGTTGATTTGCCAGGGTACGGCTTTGCTTATGCTAAAGAAGAAGTTAAGGATGCTTGGGTGGAGCTTGTGAAAGAGTATGTATCCACAAGAGTTGGTCTAAAACGAGTGTGTTTACTTATTGACACAAAATGGGGAATGAAGCCAAGGGATCATGAACTCATTGACTTGATGGAAAGGGCTGAAACTAAATATCAGATTTTATTGACGAAGACGGATACAGTTTTCCCAATTGATGTGGCACGTCGTGCAATGCAAATTGAAGAGAGTCTGAAAGCAAATAAGTCTTTGGTCCAACCTGCGGTGATGGTGAGCTCGAAATCTGGAGCTGGTATTCGAAGTTTAAGAACAGTGCTAGCAAAGATTGCTCGTGGTTATGTACTTCTTGGCCGTGGCGGGCTGCTTGTACAATTGTTTTGGCTTGGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MMR_HSR1

Protein Analysis

569

Amino Acids

64.1

Weight (kDa)

9.89

Isoelectric Point (pI)

51.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MMR_HSR1 PF01926 378 - 496 2.7e-19 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1582
AccI GTMKAC 1 cut(s) 952
AciI CCGC 3 cut(s) 1089, 1577, 1671
AclI AACGTT 2 cut(s) 424, 571
AclWI GGATC 3 cut(s) 770, 1270, 1434
AcoI YGGCCR 1 cut(s) 1663
AcsI RAATTY 1 cut(s) 765
AcuI CTGAAG 1 cut(s) 698
AdeI CACNNNGTG 1 cut(s) 1523
AfaI GTAC 4 cut(s) 1209, 1295, 1656, 1683
AfiI CCNNNNNNNGG 3 cut(s) 322, 1295, 1576
AgsI TTSAA 4 cut(s) 255, 718, 1255, 1538
AjiI CACGTC 1 cut(s) 1520
AjnI CCWGG 3 cut(s) 508, 1225, 1288
Alw21I GWGCWC 3 cut(s) 361, 676, 1592
Alw26I GTCTC 1 cut(s) 454
Alw44I GTGCAC 1 cut(s) 672
AlwI GGATC 3 cut(s) 770, 1270, 1434
AoxI GGCC 2 cut(s) 62, 1663
ApaLI GTGCAC 1 cut(s) 672
ApeKI GCWGC 2 cut(s) 1091, 1675
ApoI RAATTY 1 cut(s) 765
AseI ATTAAT 1 cut(s) 1245
AspA2I CCTAGG 1 cut(s) 434
AspS9I GGNCC 3 cut(s) 63, 308, 1567
AsuHPI GGTGA 6 cut(s) 215, 242, 350, 745, 1591, 1597
AsuII TTCGAA 1 cut(s) 1612
AsuNHI GCTAGC 1 cut(s) 1630
AvaII GGWCC 2 cut(s) 308, 1567
AvrII CCTAGG 1 cut(s) 434
BaeGI GKGCMC 1 cut(s) 676
BaeI ACNNNNGTAYC 2 cut(s) 801, 834
BanII GRGCYC 2 cut(s) 361, 1592
BauI CACGAG 1 cut(s) 1644
BbsI GAAGAC 2 cut(s) 252, 1493
Bbv12I GWGCWC 3 cut(s) 361, 676, 1592
BbvI GCAGC 2 cut(s) 1078, 1662
BccI CCATC 8 cut(s) 74, 320, 329, 467, 631, 650, 1441, 1576
BceAI ACGGC 2 cut(s) 1312, 1650
BciT130I CCWGG 3 cut(s) 510, 1227, 1290
BciVI GTATCC 2 cut(s) 1369, 1486
BclI TGATCA 1 cut(s) 742
BcoDI GTCTC 1 cut(s) 454
BfmI CTRYAG 1 cut(s) 6
BfuAI ACCTGC 1 cut(s) 1582
BfuI GTATCC 2 cut(s) 1369, 1486
BglII AGATCT 1 cut(s) 166
BisI GCNGC 3 cut(s) 1089, 1092, 1676
BlnI CCTAGG 1 cut(s) 434
BlsI GCNGC 3 cut(s) 1090, 1093, 1677
Bme1390I CCNGG 3 cut(s) 510, 1227, 1290
Bme18I GGWCC 2 cut(s) 308, 1567
BmgBI CACGTC 1 cut(s) 1520
BmgT120I GGNCC 3 cut(s) 63, 308, 1567
BmrFI CCNGG 3 cut(s) 510, 1227, 1290
BmsI GCATC 4 cut(s) 59, 287, 523, 1318
BmtI GCTAGC 1 cut(s) 1634
BpiI GAAGAC 2 cut(s) 252, 1493
BpmI CTGGAG 1 cut(s) 1620
Bpu14I TTCGAA 1 cut(s) 1612
BsaJI CCNNGG 5 cut(s) 434, 509, 1289, 1421, 1666
BsaXI ACNNNNNCTCC 2 cut(s) 343, 373
Bsc4I CCNNNNNNNGG 3 cut(s) 322, 1295, 1576
Bse1I ACTGG 1 cut(s) 606
Bse3DI GCAATG 1 cut(s) 1533
BseBI CCWGG 3 cut(s) 510, 1227, 1290
BseDI CCNNGG 5 cut(s) 434, 509, 1289, 1421, 1666
BseGI GGATG 5 cut(s) 97, 331, 478, 514, 1333
BseLI CCNNNNNNNGG 3 cut(s) 322, 1295, 1576
BseMI GCAATG 1 cut(s) 1533
BseMII CTCAG 4 cut(s) 138, 981, 1047, 1250
BseNI ACTGG 1 cut(s) 606
BseRI GAGGAG 2 cut(s) 869, 985
BseSI GKGCMC 1 cut(s) 676
BseXI GCAGC 2 cut(s) 1078, 1662
BshFI GGCC 2 cut(s) 64, 1665
BsiHKAI GWGCWC 3 cut(s) 361, 676, 1592
BsiSI CCGG 1 cut(s) 701
BslFI GGGAC 2 cut(s) 194, 426
BslI CCNNNNNNNGG 3 cut(s) 322, 1295, 1576
BsmAI GTCTC 1 cut(s) 454
BsmFI GGGAC 2 cut(s) 194, 426
BsmI GAATGC 2 cut(s) 409, 556
BsnI GGCC 2 cut(s) 64, 1665
Bsp119I TTCGAA 1 cut(s) 1612
Bsp1286I GDGCHC 3 cut(s) 361, 676, 1592
Bsp1407I TGTACA 1 cut(s) 1681
Bsp143I GATC 7 cut(s) 166, 244, 742, 775, 1123, 1262, 1426
BspACI CCGC 3 cut(s) 1089, 1577, 1671
BspANI GGCC 2 cut(s) 64, 1665
BspCNI CTCAG 4 cut(s) 139, 982, 1046, 1249
BspHI TCATGA 1 cut(s) 1429
BspMAI CTGCAG 1 cut(s) 10
BspMI ACCTGC 1 cut(s) 1582
BspOI GCTAGC 1 cut(s) 1634
BspPI GGATC 3 cut(s) 770, 1270, 1434
BspT104I TTCGAA 1 cut(s) 1612
BsrDI GCAATG 1 cut(s) 1533
BsrGI TGTACA 1 cut(s) 1681
BsrI ACTGG 1 cut(s) 606
BssECI CCNNGG 5 cut(s) 434, 509, 1289, 1421, 1666
BssMI GATC 7 cut(s) 166, 244, 742, 775, 1123, 1262, 1426
BssSI CACGAG 1 cut(s) 1644
BssT1I CCWWGG 2 cut(s) 434, 1421
Bst2BI CACGAG 1 cut(s) 1644
Bst2UI CCWGG 3 cut(s) 510, 1227, 1290
Bst4CI ACNGT 4 cut(s) 77, 888, 1498, 1627
Bst6I CTCTTC 2 cut(s) 749, 1533
BstAPI GCANNNNNTGC 2 cut(s) 1523, 1640
BstAUI TGTACA 1 cut(s) 1681
BstBI TTCGAA 1 cut(s) 1612
BstC8I GCNNGC 3 cut(s) 979, 1632, 1673
BstDEI CTNAG 5 cut(s) 147, 582, 990, 1033, 1236
BstDSI CCRYGG 1 cut(s) 1666
BstEII GGTNACC 1 cut(s) 230
BstF5I GGATG 5 cut(s) 97, 331, 478, 514, 1333
BstKTI GATC 7 cut(s) 169, 247, 745, 778, 1126, 1265, 1429
BstMAI GTCTC 1 cut(s) 454
BstMBI GATC 7 cut(s) 166, 244, 742, 775, 1123, 1262, 1426
BstMWI GCNNNNNNNGC 2 cut(s) 1523, 1640
BstNI CCWGG 3 cut(s) 510, 1227, 1290
BstPI GGTNACC 1 cut(s) 230
BstSCI CCNGG 3 cut(s) 508, 1225, 1288
BstSFI CTRYAG 1 cut(s) 6
BstSLI GKGCMC 1 cut(s) 676
BstV1I GCAGC 2 cut(s) 1078, 1662
BstV2I GAAGAC 2 cut(s) 252, 1493
BstX2I RGATCY 2 cut(s) 166, 775
BstYI RGATCY 2 cut(s) 166, 775
BsuI GTATCC 2 cut(s) 1369, 1486
BsuRI GGCC 2 cut(s) 64, 1665
BtgI CCRYGG 1 cut(s) 1666
BtrI CACGTC 1 cut(s) 1520
BtsCI GGATG 5 cut(s) 97, 331, 478, 514, 1333
BtsIMutI CAGTG 4 cut(s) 82, 674, 987, 1632
BveI ACCTGC 1 cut(s) 1582
Cac8I GCNNGC 3 cut(s) 979, 1632, 1673
CciI TCATGA 1 cut(s) 1429
Cfr13I GGNCC 3 cut(s) 63, 308, 1567
CseI GACGC 2 cut(s) 356, 963
Csp6I GTAC 4 cut(s) 1208, 1294, 1655, 1682
CspCI CAANNNNNGTGG 4 cut(s) 1351, 1386, 1494, 1529
CviAII CATG 2 cut(s) 196, 1430
CviQI GTAC 4 cut(s) 1208, 1294, 1655, 1682
DdeI CTNAG 5 cut(s) 147, 582, 990, 1033, 1236
DpnI GATC 7 cut(s) 168, 246, 744, 777, 1125, 1264, 1428
DpnII GATC 7 cut(s) 166, 244, 742, 775, 1123, 1262, 1426
DraI TTTAAA 1 cut(s) 190
DraIII CACNNNGTG 1 cut(s) 1523
EaeI YGGCCR 1 cut(s) 1663
Eam1104I CTCTTC 2 cut(s) 749, 1533
EarI CTCTTC 2 cut(s) 749, 1533
Ecl136II GAGCTC 2 cut(s) 359, 1590
Eco130I CCWWGG 2 cut(s) 434, 1421
Eco24I GRGCYC 2 cut(s) 361, 1592
Eco32I GATATC 1 cut(s) 787
Eco47I GGWCC 2 cut(s) 308, 1567
Eco53kI GAGCTC 2 cut(s) 359, 1590
Eco57I CTGAAG 1 cut(s) 698
Eco91I GGTNACC 1 cut(s) 230
EcoICRI GAGCTC 2 cut(s) 359, 1590
EcoO65I GGTNACC 1 cut(s) 230
EcoRI GAATTC 1 cut(s) 765
EcoRII CCWGG 3 cut(s) 508, 1225, 1288
EcoRV GATATC 1 cut(s) 787
EcoT14I CCWWGG 2 cut(s) 434, 1421
EcoT38I GRGCYC 2 cut(s) 361, 1592
ErhI CCWWGG 2 cut(s) 434, 1421
FaeI CATG 2 cut(s) 199, 1433
FalI AAGNNNNNCTT 6 cut(s) 98, 130, 698, 730, 1259, 1291
FaqI GGGAC 2 cut(s) 194, 426
FatI CATG 2 cut(s) 195, 1429
FauI CCCGC 1 cut(s) 1664
FbaI TGATCA 1 cut(s) 742
FblI GTMKAC 1 cut(s) 952
Fnu4HI GCNGC 3 cut(s) 1089, 1092, 1676
FokI GGATG 5 cut(s) 104, 338, 485, 501, 1340
FriOI GRGCYC 2 cut(s) 361, 1592
Fsp4HI GCNGC 3 cut(s) 1089, 1092, 1676
GluI GCNGC 3 cut(s) 1089, 1092, 1676
GsuI CTGGAG 1 cut(s) 1620
HaeIII GGCC 2 cut(s) 64, 1665
HapII CCGG 1 cut(s) 701
HgaI GACGC 2 cut(s) 356, 963
Hin1II CATG 2 cut(s) 199, 1433
HinfI GANTC 9 cut(s) 157, 222, 251, 410, 439, 503, 641, 725, 1543
HpaII CCGG 1 cut(s) 701
HphI GGTGA 6 cut(s) 215, 242, 350, 745, 1591, 1597
Hpy166II GTNNAC 3 cut(s) 674, 953, 1391
Hpy188I TCNGA 9 cut(s) 148, 288, 415, 562, 1036, 1219, 1239, 1473, 1548
Hpy188III TCNNGA 9 cut(s) 170, 248, 264, 715, 1121, 1130, 1162, 1430, 1599
Hpy8I GTNNAC 3 cut(s) 674, 953, 1391
Hpy99I CGWCG 1 cut(s) 1524
HpyAV CCTTC 7 cut(s) 54, 100, 118, 146, 283, 835, 1029
HpyCH4III ACNGT 4 cut(s) 77, 888, 1498, 1627
HpyCH4IV ACGT 4 cut(s) 424, 571, 1214, 1519
HpyF10VI GCNNNNNNNGC 2 cut(s) 1523, 1640
HpyF3I CTNAG 5 cut(s) 147, 582, 990, 1033, 1236
HpySE526I ACGT 4 cut(s) 424, 571, 1214, 1519
Hsp92II CATG 2 cut(s) 199, 1433
Ksp22I TGATCA 1 cut(s) 742
Kzo9I GATC 7 cut(s) 166, 244, 742, 775, 1123, 1262, 1426
LmnI GCTCC 4 cut(s) 111, 364, 1339, 1601
Lsp1109I GCAGC 2 cut(s) 1078, 1662
LweI GCATC 4 cut(s) 59, 287, 523, 1318
MaeII ACGT 4 cut(s) 424, 571, 1214, 1519
MaeIII GTNAC 1 cut(s) 230
MalI GATC 7 cut(s) 168, 246, 744, 777, 1125, 1264, 1428
MboI GATC 7 cut(s) 166, 244, 742, 775, 1123, 1262, 1426
MfeI CAATTG 2 cut(s) 1506, 1685
MflI RGATCY 2 cut(s) 166, 775
MhlI GDGCHC 3 cut(s) 361, 676, 1592
MluCI AATT 9 cut(s) 17, 142, 765, 937, 1073, 1246, 1506, 1533, 1685
MlyI GAGTC 3 cut(s) 166, 448, 1552
MmeI TCCRAC 1 cut(s) 1594
MslI CAYNNNNRTG 1 cut(s) 78
MspA1I CMGCKG 1 cut(s) 1091
MspI CCGG 1 cut(s) 701
MspR9I CCNGG 3 cut(s) 510, 1227, 1290
MunI CAATTG 2 cut(s) 1506, 1685
Mva1269I GAATGC 2 cut(s) 409, 556
MvaI CCWGG 3 cut(s) 510, 1227, 1290
MwoI GCNNNNNNNGC 2 cut(s) 1523, 1640
NdeII GATC 7 cut(s) 166, 244, 742, 775, 1123, 1262, 1426
NheI GCTAGC 1 cut(s) 1630
NlaIII CATG 2 cut(s) 199, 1433
NmuCI GTSAC 1 cut(s) 230
NspV TTCGAA 1 cut(s) 1612
PagI TCATGA 1 cut(s) 1429
PctI GAATGC 2 cut(s) 409, 556
PfeI GAWTC 6 cut(s) 222, 251, 410, 503, 641, 725
PkrI GCNGC 3 cut(s) 1090, 1093, 1677
PleI GAGTC 3 cut(s) 165, 447, 1551
PpsI GAGTC 3 cut(s) 165, 447, 1551
PshBI ATTAAT 1 cut(s) 1245
Psp124BI GAGCTC 2 cut(s) 361, 1592
Psp1406I AACGTT 2 cut(s) 424, 571
Psp6I CCWGG 3 cut(s) 508, 1225, 1288
PspEI GGTNACC 1 cut(s) 230
PspGI CCWGG 3 cut(s) 508, 1225, 1288
PspPI GGNCC 3 cut(s) 63, 308, 1567
PstI CTGCAG 1 cut(s) 10
PsuI RGATCY 2 cut(s) 166, 775
RsaI GTAC 4 cut(s) 1209, 1295, 1656, 1683
RsaNI GTAC 4 cut(s) 1208, 1294, 1655, 1682
RseI CAYNNNNRTG 1 cut(s) 78
SacI GAGCTC 2 cut(s) 361, 1592
SatI GCNGC 3 cut(s) 1089, 1092, 1676
Sau3AI GATC 7 cut(s) 166, 244, 742, 775, 1123, 1262, 1426
Sau96I GGNCC 3 cut(s) 63, 308, 1567
SchI GAGTC 3 cut(s) 166, 448, 1552
ScrFI CCNGG 3 cut(s) 510, 1227, 1290
SduI GDGCHC 3 cut(s) 361, 676, 1592
SfaNI GCATC 4 cut(s) 59, 287, 523, 1318
SfcI CTRYAG 1 cut(s) 6
SfuI TTCGAA 1 cut(s) 1612
SinI GGWCC 2 cut(s) 308, 1567
SmiMI CAYNNNNRTG 1 cut(s) 78
Sse9I AATT 9 cut(s) 17, 142, 765, 937, 1073, 1246, 1506, 1533, 1685
SsiI CCGC 3 cut(s) 1089, 1577, 1671
SspI AATATT 2 cut(s) 1015, 1048
SstI GAGCTC 2 cut(s) 361, 1592
StyD4I CCNGG 3 cut(s) 508, 1225, 1288
StyI CCWWGG 2 cut(s) 434, 1421
TaaI ACNGT 4 cut(s) 77, 888, 1498, 1627
TaiI ACGT 4 cut(s) 427, 574, 1217, 1522
TaqI TCGA 3 cut(s) 769, 1592, 1612
TaqII GACCGA 1 cut(s) 325
TasI AATT 9 cut(s) 17, 142, 765, 937, 1073, 1246, 1506, 1533, 1685
TatI WGTACW 2 cut(s) 1654, 1681
TauI GCSGC 1 cut(s) 1091
TfiI GAWTC 6 cut(s) 222, 251, 410, 503, 641, 725
TscAI CASTG 4 cut(s) 82, 681, 994, 1632
TseFI GTSAC 1 cut(s) 230
TseI GCWGC 2 cut(s) 1091, 1675
Tsp45I GTSAC 1 cut(s) 230
TspDTI ATGAA 7 cut(s) 128, 212, 501, 735, 1092, 1430, 1446
TspGWI ACGGA 1 cut(s) 1505
TspRI CASTG 4 cut(s) 82, 681, 994, 1632
VneI GTGCAC 1 cut(s) 672
VpaK11BI GGWCC 2 cut(s) 308, 1567
VspI ATTAAT 1 cut(s) 1245
XapI RAATTY 1 cut(s) 765
XbaI TCTAGA 1 cut(s) 263
XcmI CCANNNNNNNNNTGG 1 cut(s) 323
XmaJI CCTAGG 1 cut(s) 434
XmiI GTMKAC 1 cut(s) 952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.