RchiOBHm_Chr5g0073721

Ferrous iron transport protein B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002736
Physical Location & Seq
Forward (+)
79772475 .. 79777293
4819 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34860

Sequence Viewer

Length: 1830 bp
ATGTCTGCAGAGGAAAAATTAAGGTTTAAGTTCAATAAGAAACTCAAAATAGCAGTTGGAAATTCTAAGTTTTCAAATAGTGGGAGAAAGAAGGCTGAAGATAATGTCAATGAGAGTGAGAATGGTAAGAGAGAGAACTATAAATCGAGTGTAAGAGATAAGAAGTTTCATACTGCTGAAGAGGGGAGTCGGGGAGTTTTTGATAGAAGGAAGAGAATCTATGTTGATCGAGATGCAGCTTGGGAGGCTAACAGTAATGAGGAGGCACCTTATAGGAAGGTTTTATCTAAAAAAGGACAGAATCAATTGAAGTATGGTTATATTAATGAGAAGAACTACAGTAGATCTCCTTCACGTCCTCGTTACAAGTGGGGTTCTAATGAGAGTGCTTCTGAAACTAAATGGTTTTCTCCCAAGGAGAGAAGTGCAAAAGTTTATAATAACTTCGGCAATGAAAAGAATAATAGTAGATTTCCTTCTTGTCCCCGTTCGAAGTGGGATTCTGATGGATTAGAGAATGCTTCTGAAACTAAACGTTTTTCTCCCAAGGTTAGAAGTACAAAAGTTTACAATGAAACAAAAGTTAGGGACGCTGATAATTTTGGTAATGAAAAGAGTAGCAGCAGATCTCCTTCGCGTCCTCGCTCTACATGGGGTTCTGATATCACTGCTTCTGAAACTAAACGTTTTTTTCCTAAGGTTAGAATTGAGAAACGTTCCAATAAAACCAAAGTTAAGGATGTTGATAAGATGGAATCTATTACAACTGATGGTGGACGAAGTGCTTCACTGAAGAAACATAACCAAATAAAACCAGACTTGCAAAAATTCCCAAATATATCTCTTCCAAATACTGTGAAGAAAAAAGCATGGGAAAAGAATATCTCGGATGAAGGATCAGATGTGCTAGATGGTCAGCGAAAGAAGAAACGAATGCGACCAGATCCATATGACATATCAAATAAACGACTTGATGATAGTATTGCTAAAATTGAAAACTTAAAGGAGAAGGAAAAAGATGTTGAGGAAAAAGCTGAGATATCAAAGAATGCACAATTTCGTGCAATCCAACCAAGTCCATCAATCCTTTCCTATGTCGAAGATAATTTGTTGGGTCGTAGACGCTTGATTGAGCTGAGGAGGGCAGGGTACAACACTGAGCTTTCTGCACCATTGGATGATACTCCTTTCTCTACCAGCTCAGAAAGAGAGCGGATTGAGGAAAATATATTTAGGAGTAAATTGACATTTTTTGCTGCTGCAAAAGTTTCATCATCATTTCCACCTCCTGATCTTCCGGAGATTGCATTTGCAGGAAGGTCAAATGTTGGGAAATCATCACTACTTAATGCACTTACTAGAAATTGGGGTGTTGCACGAACATCAGATAAGCCTGGTCTTACTCAGAGTATTAATTTCTTCAATCTTGGATCAAAGCTCTGTTTGGTTGATTTGCCTGGATATGGCTTTGCTTATGCCAAAGAAGAGGTTAAGGATGCTTGGGAGGAGCTTGTGAAGGAGTATGTTTCCACAAGAGTTGGTCTAAAACGAGTGTGCTTACTTATTGATACCAAATGGGGAATGAAGCCTAGGGATCGTGAACTCATTGACCTGATGGAAAGATCTCAAACCAAGTATCAGATCTTATTAACGAAATCGGATGCGGTTTTCCCGATTGATGTAGCACGCCGTGCAATGCAAATTGAAGAGAGCCTCAAGGCAAATAAGTCTCTGGTCCAACCTGTGATGATGATGAGCTCGAGAACTGGAGCTGGTATACGAAGTTTACGAACAGTGCTGGCAAAAATTGCTCGAGTTGCCAAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MMR_HSR1

Protein Analysis

609

Amino Acids

69.52

Weight (kDa)

9.89

Isoelectric Point (pI)

55.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MMR_HSR1 PF01926 434 - 552 1.5e-19 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 438
AccB1I GGYRCC 1 cut(s) 265
AccBSI CCGCTC 1 cut(s) 1213
AccI GTMKAC 2 cut(s) 1120, 1777
AccII CGCG 1 cut(s) 637
AccIII TCCGGA 1 cut(s) 1297
AciI CCGC 2 cut(s) 1213, 1664
AclI AACGTT 3 cut(s) 535, 685, 715
AclWI GGATC 4 cut(s) 904, 938, 1438, 1602
AcsI RAATTY 2 cut(s) 61, 827
AcuI CTGAAG 3 cut(s) 117, 198, 812
AdeI CACNNNGTG 1 cut(s) 1691
AfaI GTAC 2 cut(s) 559, 1151
AfiI CCNNNNNNNGG 1 cut(s) 1463
AgsI TTSAA 6 cut(s) 34, 75, 310, 995, 1423, 1706
AjiI CACGTC 1 cut(s) 356
AjnI CCWGG 2 cut(s) 1393, 1456
AluBI AGCT 9 cut(s) 239, 1034, 1135, 1162, 1200, 1438, 1510, 1758, 1772
AluI AGCT 9 cut(s) 239, 1034, 1135, 1162, 1200, 1438, 1510, 1758, 1772
Alw21I GWGCWC 1 cut(s) 1760
Alw26I GTCTC 1 cut(s) 1734
AlwI GGATC 4 cut(s) 904, 938, 1438, 1602
Ama87I CYCGRG 2 cut(s) 1759, 1812
Aor13HI TCCGGA 1 cut(s) 1297
ApeKI GCWGC 4 cut(s) 236, 621, 1256, 1259
ApoI RAATTY 2 cut(s) 61, 827
AseI ATTAAT 2 cut(s) 324, 1413
Asp700I GAANNNNTTC 1 cut(s) 784
AspA2I CCTAGG 1 cut(s) 1589
AspS9I GGNCC 1 cut(s) 1735
AsuII TTCGAA 1 cut(s) 491
AvaI CYCGRG 2 cut(s) 1759, 1812
AvaII GGWCC 1 cut(s) 1735
AvrII CCTAGG 1 cut(s) 1589
AxyI CCTNAGG 1 cut(s) 696
BanI GGYRCC 1 cut(s) 265
BanII GRGCYC 1 cut(s) 1760
BarI GAAGNNNNNNTAC 4 cut(s) 334, 366, 460, 492
Bbv12I GWGCWC 1 cut(s) 1760
BbvCI CCTCAGC 1 cut(s) 1136
BbvI GCAGC 4 cut(s) 248, 633, 1243, 1246
BccI CCATC 6 cut(s) 500, 745, 764, 905, 1087, 1609
BceAI ACGGC 1 cut(s) 1674
BciT130I CCWGG 2 cut(s) 1395, 1458
BcoDI GTCTC 1 cut(s) 1734
BfaI CTAG 3 cut(s) 908, 1359, 1590
BfmI CTRYAG 2 cut(s) 6, 337
BglII AGATCT 4 cut(s) 344, 626, 1622, 1641
BisI GCNGC 4 cut(s) 237, 622, 1257, 1260
BlnI CCTAGG 1 cut(s) 1589
BlsI GCNGC 4 cut(s) 238, 623, 1258, 1261
Bme1390I CCNGG 2 cut(s) 1395, 1458
Bme18I GGWCC 1 cut(s) 1735
BmeT110I CYCGRG 2 cut(s) 1759, 1812
BmgBI CACGTC 1 cut(s) 356
BmgT120I GGNCC 1 cut(s) 1735
BmiI GGNNCC 1 cut(s) 267
BmrFI CCNGG 2 cut(s) 1395, 1458
BmsI GCATC 3 cut(s) 223, 1486, 1651
BpmI CTGGAG 1 cut(s) 1788
Bpu10I CCTNAGC 1 cut(s) 1136
Bpu14I TTCGAA 1 cut(s) 491
BpuEI CTTGAG 1 cut(s) 1700
BsaJI CCNNGG 3 cut(s) 414, 546, 1589
BsaWI WCCGGW 1 cut(s) 1297
Bsc4I CCNNNNNNNGG 1 cut(s) 1463
Bse1I ACTGG 1 cut(s) 1771
Bse21I CCTNAGG 1 cut(s) 696
Bse3DI GCAATG 2 cut(s) 457, 1701
BseAI TCCGGA 1 cut(s) 1297
BseBI CCWGG 2 cut(s) 1395, 1458
BseDI CCNNGG 3 cut(s) 414, 546, 1589
BseGI GGATG 5 cut(s) 745, 895, 1183, 1501, 1666
BseLI CCNNNNNNNGG 1 cut(s) 1463
BseMI GCAATG 2 cut(s) 457, 1701
BseMII CTCAG 5 cut(s) 1026, 1127, 1149, 1215, 1418
BseNI ACTGG 1 cut(s) 1771
BseRI GAGGAG 3 cut(s) 275, 1153, 1520
BseXI GCAGC 4 cut(s) 248, 633, 1243, 1246
BsgI GTGCAG 1 cut(s) 1152
Bsh1236I CGCG 1 cut(s) 637
BshNI GGYRCC 1 cut(s) 265
BsiHKAI GWGCWC 1 cut(s) 1760
BsiHKCI CYCGRG 2 cut(s) 1759, 1812
BsiSI CCGG 1 cut(s) 1298
BslFI GGGAC 2 cut(s) 468, 602
BslI CCNNNNNNNGG 1 cut(s) 1463
BsmAI GTCTC 1 cut(s) 1734
BsmFI GGGAC 2 cut(s) 468, 602
BsmI GAATGC 3 cut(s) 523, 939, 1054
BsoBI CYCGRG 2 cut(s) 1759, 1812
Bsp119I TTCGAA 1 cut(s) 491
Bsp1286I GDGCHC 1 cut(s) 1760
Bsp13I TCCGGA 1 cut(s) 1297
BspACI CCGC 2 cut(s) 1213, 1664
BspCNI CTCAG 5 cut(s) 1027, 1128, 1150, 1214, 1417
BspEI TCCGGA 1 cut(s) 1297
BspFNI CGCG 1 cut(s) 637
BspLI GGNNCC 1 cut(s) 267
BspMAI CTGCAG 1 cut(s) 10
BspPI GGATC 4 cut(s) 904, 938, 1438, 1602
BspT104I TTCGAA 1 cut(s) 491
BspT107I GGYRCC 1 cut(s) 265
BsrBI CCGCTC 1 cut(s) 1213
BsrDI GCAATG 2 cut(s) 457, 1701
BsrI ACTGG 1 cut(s) 1771
BssECI CCNNGG 3 cut(s) 414, 546, 1589
BssNAI GTATAC 1 cut(s) 1778
BssT1I CCWWGG 3 cut(s) 414, 546, 1589
Bst1107I GTATAC 1 cut(s) 1778
Bst2UI CCWGG 2 cut(s) 1395, 1458
Bst4CI ACNGT 4 cut(s) 254, 341, 856, 1795
Bst6I CTCTTC 5 cut(s) 174, 206, 849, 1479, 1701
BstAPI GCANNNNNTGC 2 cut(s) 1691, 1808
BstBI TTCGAA 1 cut(s) 491
BstC8I GCNNGC 2 cut(s) 1687, 1800
BstDEI CTNAG 7 cut(s) 66, 696, 1035, 1136, 1158, 1201, 1404
BstF5I GGATG 5 cut(s) 745, 895, 1183, 1501, 1666
BstFNI CGCG 1 cut(s) 637
BstMAI GTCTC 1 cut(s) 1734
BstMWI GCNNNNNNNGC 4 cut(s) 245, 1691, 1808, 1817
BstNI CCWGG 2 cut(s) 1395, 1458
BstSCI CCNGG 2 cut(s) 1393, 1456
BstSFI CTRYAG 2 cut(s) 6, 337
BstUI CGCG 1 cut(s) 637
BstV1I GCAGC 4 cut(s) 248, 633, 1243, 1246
BstX2I RGATCY 5 cut(s) 344, 626, 943, 1622, 1641
BstYI RGATCY 5 cut(s) 344, 626, 943, 1622, 1641
BstZ17I GTATAC 1 cut(s) 1778
Bsu36I CCTNAGG 1 cut(s) 696
BtrI CACGTC 1 cut(s) 356
BtsCI GGATG 5 cut(s) 745, 895, 1183, 1501, 1666
BtsI GCAGTG 1 cut(s) 666
BtsIMutI CAGTG 4 cut(s) 666, 788, 1155, 1800
Cac8I GCNNGC 2 cut(s) 1687, 1800
Cfr13I GGNCC 1 cut(s) 1735
CseI GACGC 3 cut(s) 599, 626, 1131
Csp6I GTAC 2 cut(s) 558, 1150
CspCI CAANNNNNGTGG 2 cut(s) 1519, 1554
CviAII CATG 2 cut(s) 651, 870
CviQI GTAC 2 cut(s) 558, 1150
DdeI CTNAG 7 cut(s) 66, 696, 1035, 1136, 1158, 1201, 1404
DraIII CACNNNGTG 1 cut(s) 1691
Eam1104I CTCTTC 5 cut(s) 174, 206, 849, 1479, 1701
EarI CTCTTC 5 cut(s) 174, 206, 849, 1479, 1701
Ecl136II GAGCTC 1 cut(s) 1758
Eco130I CCWWGG 3 cut(s) 414, 546, 1589
Eco24I GRGCYC 1 cut(s) 1760
Eco32I GATATC 2 cut(s) 664, 1041
Eco47I GGWCC 1 cut(s) 1735
Eco53kI GAGCTC 1 cut(s) 1758
Eco57I CTGAAG 3 cut(s) 117, 198, 812
Eco81I CCTNAGG 1 cut(s) 696
Eco88I CYCGRG 2 cut(s) 1759, 1812
EcoICRI GAGCTC 1 cut(s) 1758
EcoRII CCWGG 2 cut(s) 1393, 1456
EcoRV GATATC 2 cut(s) 664, 1041
EcoT14I CCWWGG 3 cut(s) 414, 546, 1589
EcoT38I GRGCYC 1 cut(s) 1760
ErhI CCWWGG 3 cut(s) 414, 546, 1589
FaeI CATG 2 cut(s) 654, 873
FaqI GGGAC 2 cut(s) 468, 602
FatI CATG 2 cut(s) 650, 869
FauNDI CATATG 1 cut(s) 949
FblI GTMKAC 2 cut(s) 1120, 1777
Fnu4HI GCNGC 4 cut(s) 237, 622, 1257, 1260
FokI GGATG 5 cut(s) 752, 902, 1190, 1508, 1673
FriOI GRGCYC 1 cut(s) 1760
Fsp4HI GCNGC 4 cut(s) 237, 622, 1257, 1260
FspBI CTAG 3 cut(s) 908, 1359, 1590
GluI GCNGC 4 cut(s) 237, 622, 1257, 1260
GsuI CTGGAG 1 cut(s) 1788
HapII CCGG 1 cut(s) 1298
HgaI GACGC 3 cut(s) 599, 626, 1131
Hin1II CATG 2 cut(s) 654, 873
HinfI GANTC 5 cut(s) 187, 216, 301, 500, 755
HpaII CCGG 1 cut(s) 1298
Hpy166II GTNNAC 6 cut(s) 568, 776, 1121, 1601, 1778, 1787
Hpy188III TCNNGA 6 cut(s) 230, 1289, 1298, 1598, 1672, 1761
Hpy8I GTNNAC 6 cut(s) 568, 776, 1121, 1601, 1778, 1787
HpyCH4III ACNGT 4 cut(s) 254, 341, 856, 1795
HpyCH4IV ACGT 4 cut(s) 355, 535, 685, 715
HpyF10VI GCNNNNNNNGC 4 cut(s) 245, 1691, 1808, 1817
HpyF3I CTNAG 7 cut(s) 66, 696, 1035, 1136, 1158, 1201, 1404
HpySE526I ACGT 4 cut(s) 355, 535, 685, 715
Hsp92II CATG 2 cut(s) 654, 873
Kpn2I TCCGGA 1 cut(s) 1297
LmnI GCTCC 2 cut(s) 1507, 1769
Lsp1109I GCAGC 4 cut(s) 248, 633, 1243, 1246
LweI GCATC 3 cut(s) 223, 1486, 1651
MaeI CTAG 3 cut(s) 908, 1359, 1590
MaeII ACGT 4 cut(s) 355, 535, 685, 715
MaeIII GTNAC 1 cut(s) 362
MbiI CCGCTC 1 cut(s) 1213
MfeI CAATTG 1 cut(s) 305
MflI RGATCY 5 cut(s) 344, 626, 943, 1622, 1641
MhlI GDGCHC 1 cut(s) 1760
MlyI GAGTC 1 cut(s) 196
MmeI TCCRAC 3 cut(s) 37, 1093, 1762
MroI TCCGGA 1 cut(s) 1297
MroXI GAANNNNTTC 1 cut(s) 784
MseI TTAA 9 cut(s) 20, 27, 324, 735, 1001, 1347, 1413, 1491, 1649
MspI CCGG 1 cut(s) 1298
MspR9I CCNGG 2 cut(s) 1395, 1458
MunI CAATTG 1 cut(s) 305
Mva1269I GAATGC 3 cut(s) 523, 939, 1054
MvaI CCWGG 2 cut(s) 1395, 1458
MvnI CGCG 1 cut(s) 637
MwoI GCNNNNNNNGC 4 cut(s) 245, 1691, 1808, 1817
NdeI CATATG 1 cut(s) 949
NlaIII CATG 2 cut(s) 654, 873
NlaIV GGNNCC 1 cut(s) 267
NspV TTCGAA 1 cut(s) 491
PaeR7I CTCGAG 2 cut(s) 1759, 1812
PcsI WCGNNNNNNNCGW 1 cut(s) 1786
PctI GAATGC 3 cut(s) 523, 939, 1054
PdmI GAANNNNTTC 1 cut(s) 784
PfeI GAWTC 4 cut(s) 216, 301, 500, 755
PkrI GCNGC 4 cut(s) 238, 623, 1258, 1261
PleI GAGTC 1 cut(s) 195
PpsI GAGTC 1 cut(s) 195
PshBI ATTAAT 2 cut(s) 324, 1413
PsiI TTATAA 1 cut(s) 438
Psp124BI GAGCTC 1 cut(s) 1760
Psp1406I AACGTT 3 cut(s) 535, 685, 715
Psp6I CCWGG 2 cut(s) 1393, 1456
PspGI CCWGG 2 cut(s) 1393, 1456
PspN4I GGNNCC 1 cut(s) 267
PspPI GGNCC 1 cut(s) 1735
PspXI VCTCGAGB 1 cut(s) 1812
PstI CTGCAG 1 cut(s) 10
PsuI RGATCY 5 cut(s) 344, 626, 943, 1622, 1641
RsaI GTAC 2 cut(s) 559, 1151
RsaNI GTAC 2 cut(s) 558, 1150
SacI GAGCTC 1 cut(s) 1760
SaqAI TTAA 9 cut(s) 20, 27, 324, 735, 1001, 1347, 1413, 1491, 1649
SatI GCNGC 4 cut(s) 237, 622, 1257, 1260
Sau96I GGNCC 1 cut(s) 1735
SchI GAGTC 1 cut(s) 196
ScrFI CCNGG 2 cut(s) 1395, 1458
SduI GDGCHC 1 cut(s) 1760
SfaNI GCATC 3 cut(s) 223, 1486, 1651
SfcI CTRYAG 2 cut(s) 6, 337
Sfr274I CTCGAG 2 cut(s) 1759, 1812
SfuI TTCGAA 1 cut(s) 491
SinI GGWCC 1 cut(s) 1735
SlaI CTCGAG 2 cut(s) 1759, 1812
SmlI CTYRAG 3 cut(s) 1715, 1759, 1812
SmoI CTYRAG 3 cut(s) 1715, 1759, 1812
SsiI CCGC 2 cut(s) 1213, 1664
SspMI CTAG 3 cut(s) 908, 1359, 1590
SstI GAGCTC 1 cut(s) 1760
StyD4I CCNGG 2 cut(s) 1393, 1456
StyI CCWWGG 3 cut(s) 414, 546, 1589
TaaI ACNGT 4 cut(s) 254, 341, 856, 1795
TaiI ACGT 4 cut(s) 358, 538, 688, 718
TaqI TCGA 6 cut(s) 146, 229, 491, 1098, 1760, 1813
TatI WGTACW 1 cut(s) 557
TfiI GAWTC 4 cut(s) 216, 301, 500, 755
Tru1I TTAA 9 cut(s) 20, 27, 324, 735, 1001, 1347, 1413, 1491, 1649
Tru9I TTAA 9 cut(s) 20, 27, 324, 735, 1001, 1347, 1413, 1491, 1649
TscAI CASTG 4 cut(s) 673, 795, 1162, 1800
TseI GCWGC 4 cut(s) 236, 621, 1256, 1259
TspDTI ATGAA 7 cut(s) 158, 468, 588, 624, 906, 1260, 1598
TspRI CASTG 4 cut(s) 673, 795, 1162, 1800
VpaK11BI GGWCC 1 cut(s) 1735
VspI ATTAAT 2 cut(s) 324, 1413
XapI RAATTY 2 cut(s) 61, 827
XhoI CTCGAG 2 cut(s) 1759, 1812
XmaJI CCTAGG 1 cut(s) 1589
XmiI GTMKAC 2 cut(s) 1120, 1777
XmnI GAANNNNTTC 1 cut(s) 784
XspI CTAG 3 cut(s) 908, 1359, 1590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.