MD03G1070000.v1.1

Domain of unknown function (DUF4792)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001521
Physical Location & Seq
Reverse (-)
5719526 .. 5723165
3640 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1070000.v1.1.491

Sequence Viewer

Length: 1413 bp
ATGCTGCCTCCCAAAACTCCCATCACCCAGGTAAACATGGAAACCCCAGAACCAGATCGAAGAGAAGATCAATCCTTTCCATCATCTTCTTCTTCTTCGCCATCGAGCTCACGGGTGGAAGAAGAAGAAAGTGACAGACGTTCAAATGGGGACAATTTTGATGAGCAGCAGCGGACGAGAAGGTCTAGGACTTATCATGTGAACTTGTTGTTGTACGATGTAGAAGCGTCTGAGGTGAAGGATGATATTTGGTCGGCCCTTGCTGTGCTTGTCACCTTTTGGTTCTTTGCATCTATGACTATGATTCTTGGTTTCTATGGATCCGACAATCTACAATTGGGCCCGAACTGCTCGCGCTTGATACAAACTAATCCGTTCTTTGTGCAATCGATTAAGGAGCATGATGAGCAAAAACTTGGGCCAGTGTTATATGGATTCAACAAACCTCCTCCCTTGGATGTTGAAATAGCATGGACCGAAACACAGCATACGCTTGTTCCAGCCAATTTTCACAAGGCATGGATATACTTTCTAAACAAAGGGTCTGGTTTGGATATTACTTACCGTGTGAAGCCTACAAGTTCCTCGCCTTTAACTCTTGTAATAGCACAAGGTAGTGAGAGCCTGGCTGAATGGATAGAGGATCCATCATATGGTAATGCAACACTGTCTTGGAATATAATTTATGGAAGTGGTAGGATCCAGCAGGAAATACCAGAGTCTTCTACTTATTATATAGCAGTAGGAAACTTGAACCCTGAGAATGTTGAGGTAGAGTTGGTATTCAACATAAAATCTATTCTTTATAACACAACCGAGGCGTATTACAAATGCTCTTTGTATAACCGGTTATGTAGCTTAAAGCTTTCCCTCTTGGGGGCAAATGTTGCTGTCATAACTTCTCCAGGTCCTAAAGAGGGTATCCCTAATGATGATTGGTATGTAAGCCTGTCCTATGGACCGCGATGGGCCATGTATTTTCTTGTAGCAGGCGTTATGACTCTGCTCCTCTTAACGGCCTTTATATTTTATAACAAATTCCAGAATTCTAGTGAAGACACAACCGAATTTCAAGCAGAGGAGGGACAATCCGTGCAAACCCCGCTGCTATCACCCAAAGACGATGATACTTTGAGTTGGGGTTCGTCGTATGAATTTATTTCCAATGAAGAAGATTTGGATGAGTGCCTTGCAGTGAGTTCCCTTGAAGGAAGTTTAACAAATGAAGGAGATAGCAACACTACTGGGCGTCTTTGTGTCATATGCTTTGATGGTCCTAGGGATTGCTTCTTTCTTCCTTGTGGGCATTGCGCTGCCTGTTTTACTTGCGGGACAAGGATTGCCGAAGAGGCTGGTACGTGTCCCATATGCCGTAGAAGGATGAAGAAAGTGAGGAAGATTTTTACAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4792 DUF4793 zf-C3HC4_3

Protein Analysis

471

Amino Acids

52.78

Weight (kDa)

4.71

Isoelectric Point (pI)

54.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APD1-4_N PF16040 140 - 209 6.3e-22 E3 ubiquitin-protein ligase APD1-4, N-terminal
APD1-4_M PF16041 234 - 339 1.9e-32 E3 ubiquitin-protein ligase APD1-4, middle domain
zf-C3HC4_3 PF13920 417 - 464 5.3e-13 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 418 - 458 4.3e-06 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 807, 1032
AasI GACNNNNNNGTC 1 cut(s) 181
AccB7I CCANNNNNTGG 1 cut(s) 653
AccII CGCG 2 cut(s) 355, 964
AciI CCGC 4 cut(s) 172, 962, 1103, 1329
AclWI GGATC 6 cut(s) 315, 328, 638, 651, 694, 707
AcsI RAATTY 4 cut(s) 1037, 1045, 1067, 1154
AcyI GRCGYC 1 cut(s) 1249
AfaI GTAC 2 cut(s) 215, 1357
AfiI CCNNNNNNNGG 5 cut(s) 653, 876, 877, 917, 1015
AflIII ACRYGT 1 cut(s) 1358
AgeI ACCGGT 1 cut(s) 846
AgsI TTSAA 7 cut(s) 144, 439, 464, 754, 787, 1073, 1208
AjnI CCWGG 3 cut(s) 27, 624, 904
AluBI AGCT 3 cut(s) 108, 858, 865
AluI AGCT 3 cut(s) 108, 858, 865
Alw21I GWGCWC 1 cut(s) 110
AlwI GGATC 6 cut(s) 315, 328, 638, 651, 694, 707
AoxI GGCC 5 cut(s) 255, 340, 419, 969, 1017
ApaI GGGCCC 1 cut(s) 344
ApeKI GCWGC 5 cut(s) 4, 166, 169, 1105, 1313
ApoI RAATTY 4 cut(s) 1037, 1045, 1067, 1154
AsiGI ACCGGT 1 cut(s) 846
AspA2I CCTAGG 1 cut(s) 1277
AspLEI GCGC 2 cut(s) 357, 1313
AspS9I GGNCC 9 cut(s) 256, 340, 341, 419, 474, 908, 959, 969, 1274
AsuHPI GGTGA 4 cut(s) 16, 247, 265, 1104
AvaII GGWCC 4 cut(s) 474, 908, 959, 1274
AvrII CCTAGG 1 cut(s) 1277
BaeGI GKGCMC 1 cut(s) 344
BamHI GGATCC 3 cut(s) 320, 643, 699
BanII GRGCYC 2 cut(s) 110, 344
BbsI GAAGAC 2 cut(s) 714, 1062
Bbv12I GWGCWC 1 cut(s) 110
BbvI GCAGC 4 cut(s) 178, 181, 1092, 1300
BccI CCATC 6 cut(s) 29, 88, 109, 655, 960, 1265
BceAI ACGGC 2 cut(s) 1032, 1356
BciT130I CCWGG 3 cut(s) 29, 626, 906
BciVI GTATCC 1 cut(s) 932
BfaI CTAG 3 cut(s) 186, 1050, 1278
BfuI GTATCC 1 cut(s) 932
BglI GCCNNNNNGGC 1 cut(s) 1349
BisI GCNGC 5 cut(s) 5, 167, 170, 1106, 1314
BlnI CCTAGG 1 cut(s) 1277
BlsI GCNGC 5 cut(s) 6, 168, 171, 1107, 1315
Bme1390I CCNGG 3 cut(s) 29, 626, 906
Bme18I GGWCC 4 cut(s) 474, 908, 959, 1274
BmgT120I GGNCC 9 cut(s) 256, 340, 341, 419, 474, 908, 959, 969, 1274
BmiI GGNNCC 4 cut(s) 322, 342, 645, 701
BmrFI CCNGG 3 cut(s) 29, 626, 906
BmrI ACTGGG 1 cut(s) 1254
BmsI GCATC 1 cut(s) 299
BmuI ACTGGG 1 cut(s) 1254
BpiI GAAGAC 2 cut(s) 714, 1062
BpmI CTGGAG 1 cut(s) 888
Bsa29I ATCGAT 1 cut(s) 389
BsaAI YACGTR 1 cut(s) 1359
BsaHI GRCGYC 1 cut(s) 1249
BsaJI CCNNGG 4 cut(s) 27, 453, 816, 1277
BsaWI WCCGGW 1 cut(s) 846
Bsc4I CCNNNNNNNGG 5 cut(s) 653, 876, 877, 917, 1015
Bse118I RCCGGY 1 cut(s) 846
Bse1I ACTGG 2 cut(s) 422, 1249
Bse3DI GCAATG 1 cut(s) 1306
BseBI CCWGG 3 cut(s) 29, 626, 906
BseCI ATCGAT 1 cut(s) 389
BseDI CCNNGG 4 cut(s) 27, 453, 816, 1277
BseGI GGATG 4 cut(s) 247, 463, 1186, 1386
BseLI CCNNNNNNNGG 5 cut(s) 653, 876, 877, 917, 1015
BseMI GCAATG 1 cut(s) 1306
BseMII CTCAG 2 cut(s) 222, 750
BseNI ACTGG 2 cut(s) 422, 1249
BseRI GAGGAG 3 cut(s) 438, 998, 1094
BseSI GKGCMC 1 cut(s) 344
BseXI GCAGC 4 cut(s) 178, 181, 1092, 1300
Bsh1236I CGCG 2 cut(s) 355, 964
BshFI GGCC 5 cut(s) 257, 342, 421, 971, 1019
BshTI ACCGGT 1 cut(s) 846
BshVI ATCGAT 1 cut(s) 389
BsiHKAI GWGCWC 1 cut(s) 110
BsiSI CCGG 1 cut(s) 847
BslFI GGGAC 4 cut(s) 164, 1098, 1345, 1347
BslI CCNNNNNNNGG 5 cut(s) 653, 876, 877, 917, 1015
BsmFI GGGAC 4 cut(s) 164, 1098, 1345, 1347
BsnI GGCC 5 cut(s) 257, 342, 421, 971, 1019
Bsp120I GGGCCC 1 cut(s) 340
Bsp1286I GDGCHC 2 cut(s) 110, 344
Bsp143I GATC 5 cut(s) 55, 67, 320, 643, 699
BspACI CCGC 4 cut(s) 172, 962, 1103, 1329
BspANI GGCC 5 cut(s) 257, 342, 421, 971, 1019
BspCNI CTCAG 2 cut(s) 223, 751
BspDI ATCGAT 1 cut(s) 389
BspFNI CGCG 2 cut(s) 355, 964
BspLI GGNNCC 4 cut(s) 322, 342, 645, 701
BspPI GGATC 6 cut(s) 315, 328, 638, 651, 694, 707
BsrDI GCAATG 1 cut(s) 1306
BsrFI RCCGGY 1 cut(s) 846
BsrI ACTGG 2 cut(s) 422, 1249
BssAI RCCGGY 1 cut(s) 846
BssECI CCNNGG 4 cut(s) 27, 453, 816, 1277
BssMI GATC 5 cut(s) 55, 67, 320, 643, 699
BssNI GRCGYC 1 cut(s) 1249
BssT1I CCWWGG 2 cut(s) 453, 1277
Bst2UI CCWGG 3 cut(s) 29, 626, 906
Bst4CI ACNGT 3 cut(s) 566, 669, 1408
Bst6I CTCTTC 2 cut(s) 55, 1341
BstACI GRCGYC 1 cut(s) 1249
BstAPI GCANNNNNTGC 1 cut(s) 887
BstBAI YACGTR 1 cut(s) 1359
BstC8I GCNNGC 2 cut(s) 353, 991
BstDEI CTNAG 2 cut(s) 231, 759
BstF5I GGATG 4 cut(s) 247, 463, 1186, 1386
BstFNI CGCG 2 cut(s) 355, 964
BstHHI GCGC 2 cut(s) 357, 1313
BstKTI GATC 5 cut(s) 58, 70, 323, 646, 702
BstMBI GATC 5 cut(s) 55, 67, 320, 643, 699
BstMWI GCNNNNNNNGC 5 cut(s) 348, 406, 887, 1102, 1349
BstNI CCWGG 3 cut(s) 29, 626, 906
BstSCI CCNGG 3 cut(s) 27, 624, 904
BstSLI GKGCMC 1 cut(s) 344
BstUI CGCG 2 cut(s) 355, 964
BstV1I GCAGC 4 cut(s) 178, 181, 1092, 1300
BstV2I GAAGAC 2 cut(s) 714, 1062
BstX2I RGATCY 3 cut(s) 320, 643, 699
BstYI RGATCY 3 cut(s) 320, 643, 699
Bsu15I ATCGAT 1 cut(s) 389
BsuI GTATCC 1 cut(s) 932
BsuRI GGCC 5 cut(s) 257, 342, 421, 971, 1019
BsuTUI ATCGAT 1 cut(s) 389
BtgZI GCGATG 1 cut(s) 979
BtsCI GGATG 4 cut(s) 247, 463, 1186, 1386
BtsI GCAGTG 1 cut(s) 1200
BtsIMutI CAGTG 3 cut(s) 429, 665, 1200
Cac8I GCNNGC 2 cut(s) 353, 991
CfoI GCGC 2 cut(s) 357, 1313
Cfr10I RCCGGY 1 cut(s) 846
Cfr13I GGNCC 9 cut(s) 256, 340, 341, 419, 474, 908, 959, 969, 1274
ClaI ATCGAT 1 cut(s) 389
CseI GACGC 2 cut(s) 216, 1238
Csp6I GTAC 2 cut(s) 214, 1356
CspAI ACCGGT 1 cut(s) 846
CviAII CATG 6 cut(s) 37, 197, 401, 471, 519, 973
CviQI GTAC 2 cut(s) 214, 1356
DdeI CTNAG 2 cut(s) 231, 759
DpnI GATC 5 cut(s) 57, 69, 322, 645, 701
DpnII GATC 5 cut(s) 55, 67, 320, 643, 699
DrdI GACNNNNNNGTC 1 cut(s) 181
DseDI GACNNNNNNGTC 1 cut(s) 181
Eam1104I CTCTTC 2 cut(s) 55, 1341
EarI CTCTTC 2 cut(s) 55, 1341
Ecl136II GAGCTC 1 cut(s) 108
Eco130I CCWWGG 2 cut(s) 453, 1277
Eco24I GRGCYC 2 cut(s) 110, 344
Eco47I GGWCC 4 cut(s) 474, 908, 959, 1274
Eco53kI GAGCTC 1 cut(s) 108
EcoICRI GAGCTC 1 cut(s) 108
EcoO109I RGGNCCY 1 cut(s) 908
EcoRI GAATTC 1 cut(s) 1045
EcoRII CCWGG 3 cut(s) 27, 624, 904
EcoT14I CCWWGG 2 cut(s) 453, 1277
EcoT38I GRGCYC 2 cut(s) 110, 344
ErhI CCWWGG 2 cut(s) 453, 1277
FaeI CATG 6 cut(s) 40, 200, 404, 474, 522, 976
FaqI GGGAC 4 cut(s) 164, 1098, 1345, 1347
FatI CATG 6 cut(s) 36, 196, 400, 470, 518, 972
FauI CCCGC 2 cut(s) 1110, 1322
FauNDI CATATG 3 cut(s) 652, 1262, 1367
Fnu4HI GCNGC 5 cut(s) 5, 167, 170, 1106, 1314
FokI GGATG 4 cut(s) 254, 470, 1193, 1393
FriOI GRGCYC 2 cut(s) 110, 344
Fsp4HI GCNGC 5 cut(s) 5, 167, 170, 1106, 1314
FspBI CTAG 3 cut(s) 186, 1050, 1278
GlaI GCGC 2 cut(s) 356, 1312
GluI GCNGC 5 cut(s) 5, 167, 170, 1106, 1314
GsuI CTGGAG 1 cut(s) 888
HaeIII GGCC 5 cut(s) 257, 342, 421, 971, 1019
HapII CCGG 1 cut(s) 847
HgaI GACGC 2 cut(s) 216, 1238
HhaI GCGC 2 cut(s) 357, 1313
Hin1I GRCGYC 1 cut(s) 1249
Hin1II CATG 6 cut(s) 40, 200, 404, 474, 522, 976
Hin6I GCGC 2 cut(s) 355, 1311
HinP1I GCGC 2 cut(s) 355, 1311
HindIII AAGCTT 1 cut(s) 863
HinfI GANTC 4 cut(s) 304, 435, 719, 1000
HpaII CCGG 1 cut(s) 847
HphI GGTGA 4 cut(s) 16, 247, 265, 1104
Hpy166II GTNNAC 2 cut(s) 34, 202
Hpy188I TCNGA 2 cut(s) 232, 325
Hpy188III TCNNGA 1 cut(s) 1042
Hpy8I GTNNAC 2 cut(s) 34, 202
Hpy99I CGWCG 1 cut(s) 1150
HpyAV CCTTC 5 cut(s) 174, 232, 1202, 1220, 1371
HpyCH4III ACNGT 3 cut(s) 566, 669, 1408
HpyCH4IV ACGT 2 cut(s) 139, 1358
HpyCH4V TGCA 5 cut(s) 290, 385, 662, 1096, 1193
HpyF10VI GCNNNNNNNGC 5 cut(s) 348, 406, 887, 1102, 1349
HpyF3I CTNAG 2 cut(s) 231, 759
HpySE526I ACGT 2 cut(s) 139, 1358
Hsp92I GRCGYC 1 cut(s) 1249
Hsp92II CATG 6 cut(s) 40, 200, 404, 474, 522, 976
HspAI GCGC 2 cut(s) 355, 1311
Kzo9I GATC 5 cut(s) 55, 67, 320, 643, 699
LmnI GCTCC 2 cut(s) 397, 1011
Lsp1109I GCAGC 4 cut(s) 178, 181, 1092, 1300
LweI GCATC 1 cut(s) 299
MaeI CTAG 3 cut(s) 186, 1050, 1278
MaeII ACGT 2 cut(s) 139, 1358
MaeIII GTNAC 2 cut(s) 131, 271
MalI GATC 5 cut(s) 57, 69, 322, 645, 701
MboI GATC 5 cut(s) 55, 67, 320, 643, 699
MfeI CAATTG 1 cut(s) 335
MflI RGATCY 3 cut(s) 320, 643, 699
MhlI GDGCHC 2 cut(s) 110, 344
MluCI AATT 8 cut(s) 154, 335, 505, 681, 1037, 1045, 1067, 1154
MlyI GAGTC 2 cut(s) 728, 994
MmeI TCCRAC 1 cut(s) 348
MseI TTAA 5 cut(s) 393, 593, 860, 1013, 1217
MspA1I CMGCKG 2 cut(s) 172, 1105
MspI CCGG 1 cut(s) 847
MspR9I CCNGG 3 cut(s) 29, 626, 906
MunI CAATTG 1 cut(s) 335
MvaI CCWGG 3 cut(s) 29, 626, 906
MvnI CGCG 2 cut(s) 355, 964
MwoI GCNNNNNNNGC 5 cut(s) 348, 406, 887, 1102, 1349
NdeI CATATG 3 cut(s) 652, 1262, 1367
NdeII GATC 5 cut(s) 55, 67, 320, 643, 699
NlaIII CATG 6 cut(s) 40, 200, 404, 474, 522, 976
NlaIV GGNNCC 4 cut(s) 322, 342, 645, 701
NmuCI GTSAC 2 cut(s) 131, 271
PfeI GAWTC 2 cut(s) 304, 435
PflMI CCANNNNNTGG 1 cut(s) 653
PinAI ACCGGT 1 cut(s) 846
PkrI GCNGC 5 cut(s) 6, 168, 171, 1107, 1315
PleI GAGTC 2 cut(s) 727, 994
PpsI GAGTC 2 cut(s) 727, 994
Ppu21I YACGTR 1 cut(s) 1359
PpuMI RGGWCCY 1 cut(s) 908
PsiI TTATAA 2 cut(s) 807, 1032
Psp124BI GAGCTC 1 cut(s) 110
Psp5II RGGWCCY 1 cut(s) 908
Psp6I CCWGG 3 cut(s) 27, 624, 904
PspGI CCWGG 3 cut(s) 27, 624, 904
PspN4I GGNNCC 4 cut(s) 322, 342, 645, 701
PspOMI GGGCCC 1 cut(s) 340
PspPI GGNCC 9 cut(s) 256, 340, 341, 419, 474, 908, 959, 969, 1274
PspPPI RGGWCCY 1 cut(s) 908
PsuI RGATCY 3 cut(s) 320, 643, 699
RsaI GTAC 2 cut(s) 215, 1357
RsaNI GTAC 2 cut(s) 214, 1356
SacI GAGCTC 1 cut(s) 110
SaqAI TTAA 5 cut(s) 393, 593, 860, 1013, 1217
SatI GCNGC 5 cut(s) 5, 167, 170, 1106, 1314
Sau3AI GATC 5 cut(s) 55, 67, 320, 643, 699
Sau96I GGNCC 9 cut(s) 256, 340, 341, 419, 474, 908, 959, 969, 1274
SchI GAGTC 2 cut(s) 728, 994
ScrFI CCNGG 3 cut(s) 29, 626, 906
SduI GDGCHC 2 cut(s) 110, 344
SfaNI GCATC 1 cut(s) 299
SinI GGWCC 4 cut(s) 474, 908, 959, 1274
Sse9I AATT 8 cut(s) 154, 335, 505, 681, 1037, 1045, 1067, 1154
SsiI CCGC 4 cut(s) 172, 962, 1103, 1329
SspMI CTAG 3 cut(s) 186, 1050, 1278
SstI GAGCTC 1 cut(s) 110
StyD4I CCNGG 3 cut(s) 27, 624, 904
StyI CCWWGG 2 cut(s) 453, 1277
TaaI ACNGT 3 cut(s) 566, 669, 1408
TaiI ACGT 2 cut(s) 142, 1361
TaqI TCGA 3 cut(s) 58, 104, 389
TaqII GACCGA 1 cut(s) 491
TasI AATT 8 cut(s) 154, 335, 505, 681, 1037, 1045, 1067, 1154
TfiI GAWTC 2 cut(s) 304, 435
Tru1I TTAA 5 cut(s) 393, 593, 860, 1013, 1217
Tru9I TTAA 5 cut(s) 393, 593, 860, 1013, 1217
TscAI CASTG 3 cut(s) 429, 672, 1200
TseFI GTSAC 2 cut(s) 131, 271
TseI GCWGC 5 cut(s) 4, 166, 169, 1105, 1313
Tsp45I GTSAC 2 cut(s) 131, 271
TspDTI ATGAA 4 cut(s) 1167, 1182, 1239, 1397
TspGWI ACGGA 2 cut(s) 363, 1081
TspRI CASTG 3 cut(s) 429, 672, 1200
Van91I CCANNNNNTGG 1 cut(s) 653
VpaK11BI GGWCC 4 cut(s) 474, 908, 959, 1274
XapI RAATTY 4 cut(s) 1037, 1045, 1067, 1154
XmaJI CCTAGG 1 cut(s) 1277
XspI CTAG 3 cut(s) 186, 1050, 1278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.