RchiOBHm_Chr5g0069661

Domain of unknown function (DUF4792)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001521
Physical Location & Seq
Forward (+)
75487409 .. 75491149
3741 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34503

Sequence Viewer

Length: 1380 bp
ATGGAAGACCCAGAAACAGATCGTAGCCACCATGAATCCGTTCCTTCTTCTTCTTCGTCTTCTTCTTCTTCTTCTTCATCATCTACCTCACAGGCCCAAGAACAAACAGAGCAGCAACCAGAGCAGAGAAGTTCAACTAGGGCTTTCTCTTACCGTGTCAATGTATTGATAGCTGATGTAGCAGCATTTGACATGAAGGACGATCTTTGGTCTGGCTTTATTGTGCTCGTCACCTTTTGGTTCTTTGCATCCATGACTTTGATTCTTGGTTTCTTTGGATCCGCTGATTTACAACTGGGCCCGAATTGCTCACGTCTCATACGAACTAATCCATTCTTTGTTCAGACCATTAAGGCACAGGAACTTCAGGGTACAAAACCTGGGTCAATTTTATATGGATTCCACACACCTCCTCCTCTGGATGTTGAAACAGCTTGGACTGAAACACATCATACACTTGTGCACCCTACTTATCACAAGGAATGGATTTACTTTCTAAACAAAGGGTCTAGAATAGATATATATTACCATGTCAAACCTTCAAGTTCCTCGCCTTTAACACTTGTAATTGCAGAAGGTAGAGAAAGCCTGGTTGAATGGATAGAGGATCCATCATATGGTAATGCAACATTGTCTTGGAATATAATTTATGGAAGTGGTAAGATCCAACAGGTAATTTCAGAGTCTTCGAATTATTATATTGCAGTAGGGAACTTGAACCCTGAGGATGTTGAGGTAGAGTTGGAATTCAACATAAAGTCCACTCTTTATAACACGGCCCAGGCGTATTACAGGTGCTCCATACATAATCAATACTGTAGCTTAAAGCTTTCCCTCTTAGGGGCAACTTTTGCTGTCTTAACTTCTCCAGGTCCTGAAGAGGGTATTCGGGAGGATGACTGGTATGTCAAACTGTCATATGGACCTCGATGGGTCATCTATTTTCTTGGATCAGGTGTGATGACTGTGCTCCTGTTAATGGTCTTTAGATTTTGTAACATTTTCCAAAATGCTGGTGAGGATAGAACAGATTTTCAAGCAAGGGAGGCATCACCTGTACGAACCCCGTTGCTTTTACCCAAAGATGATGATGCTTTGAGTTGGGGTTCATCTTATGAGTGTATTTCAAATGATGAAGAGGATTTGGAAGAGTTCCTTGGAGTGAGTTCCGTTGAAGGAAAGTCGATAAATGAAAGGAACGACAACAATACTCAGCGTCTTTGTGTTATTTGCTTTGATGGGCCGAGAGACTGCTTCTTTCTTCCATGTGGACACTGTGCTACCTGTTTTACTTGTGGAACAAGGACTTCTGAAGAGGCTGGAACTTGTCCTATTTGTCGTATGAAGATGAAGAAAGTGAGGAAGATTTTTTCAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4792 DUF4793 zf-C3HC4_3

Protein Analysis

459

Amino Acids

51.63

Weight (kDa)

4.98

Isoelectric Point (pI)

44.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APD1-4_N PF16040 128 - 197 1.3e-21 E3 ubiquitin-protein ligase APD1-4, N-terminal
APD1-4_M PF16041 222 - 328 8e-33 E3 ubiquitin-protein ligase APD1-4, middle domain
zf-C3HC4_3 PF13920 405 - 453 1.5e-12 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 407 - 447 1e-05 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 771
AasI GACNNNNNNGTC 1 cut(s) 905
AccB7I CCANNNNNTGG 1 cut(s) 617
AciI CCGC 1 cut(s) 282
AclWI GGATC 6 cut(s) 273, 286, 602, 615, 658, 958
AcsI RAATTY 1 cut(s) 746
AcuI CTGAAG 3 cut(s) 350, 897, 1332
AfaI GTAC 2 cut(s) 373, 1059
AfiI CCNNNNNNNGG 5 cut(s) 617, 840, 841, 881, 979
AgsI TTSAA 9 cut(s) 135, 428, 543, 596, 718, 751, 1037, 1128, 1175
AjiI CACGTC 1 cut(s) 314
AjnI CCWGG 4 cut(s) 379, 588, 780, 868
AjuI GAANNNNNNNTTGG 2 cut(s) 1140, 1172
AluBI AGCT 4 cut(s) 173, 434, 822, 829
AluI AGCT 4 cut(s) 173, 434, 822, 829
Alw21I GWGCWC 4 cut(s) 228, 465, 800, 972
Alw26I GTCTC 2 cut(s) 320, 1242
Alw44I GTGCAC 1 cut(s) 461
AlwI GGATC 6 cut(s) 273, 286, 602, 615, 658, 958
AlwNI CAGNNNCTG 1 cut(s) 875
AoxI GGCC 4 cut(s) 93, 298, 777, 1241
ApaI GGGCCC 1 cut(s) 302
ApaLI GTGCAC 1 cut(s) 461
ApeKI GCWGC 2 cut(s) 112, 182
ApoI RAATTY 1 cut(s) 746
ArsI GACNNNNNNTTYG 2 cut(s) 368, 400
Asp700I GAANNNNTTC 1 cut(s) 39
AspS9I GGNCC 7 cut(s) 94, 298, 299, 778, 872, 923, 1241
AsuHPI GGTGA 3 cut(s) 223, 1028, 1044
AsuII TTCGAA 1 cut(s) 689
AvaII GGWCC 2 cut(s) 872, 923
AxyI CCTNAGG 1 cut(s) 723
BaeGI GKGCMC 2 cut(s) 302, 465
BamHI GGATCC 2 cut(s) 278, 607
BanII GRGCYC 1 cut(s) 302
BbsI GAAGAC 3 cut(s) 12, 51, 678
Bbv12I GWGCWC 4 cut(s) 228, 465, 800, 972
BbvI GCAGC 2 cut(s) 124, 194
BccI CCATC 3 cut(s) 619, 924, 1232
BceAI ACGGC 1 cut(s) 792
BciT130I CCWGG 4 cut(s) 381, 590, 782, 870
BcoDI GTCTC 2 cut(s) 320, 1242
BfaI CTAG 2 cut(s) 138, 510
BfmI CTRYAG 1 cut(s) 817
BisI GCNGC 2 cut(s) 113, 183
BlsI GCNGC 2 cut(s) 114, 184
Bme1390I CCNGG 4 cut(s) 381, 590, 782, 870
Bme18I GGWCC 2 cut(s) 872, 923
BmgBI CACGTC 1 cut(s) 314
BmgT120I GGNCC 7 cut(s) 94, 298, 299, 778, 872, 923, 1241
BmiI GGNNCC 3 cut(s) 280, 300, 609
BmrFI CCNGG 4 cut(s) 381, 590, 782, 870
BmrI ACTGGG 1 cut(s) 305
BmsI GCATC 3 cut(s) 257, 1058, 1081
BmuI ACTGGG 1 cut(s) 305
BpiI GAAGAC 3 cut(s) 12, 51, 678
BpmI CTGGAG 1 cut(s) 852
Bpu14I TTCGAA 1 cut(s) 689
BsaJI CCNNGG 3 cut(s) 380, 780, 1156
BsaXI ACNNNNNCTCC 4 cut(s) 397, 427, 782, 812
Bsc4I CCNNNNNNNGG 5 cut(s) 617, 840, 841, 881, 979
Bse1I ACTGG 2 cut(s) 300, 905
Bse21I CCTNAGG 1 cut(s) 723
BseBI CCWGG 4 cut(s) 381, 590, 782, 870
BseDI CCNNGG 3 cut(s) 380, 780, 1156
BseGI GGATG 4 cut(s) 248, 427, 733, 901
BseLI CCNNNNNNNGG 5 cut(s) 617, 840, 841, 881, 979
BseMII CTCAG 2 cut(s) 714, 1226
BseNI ACTGG 2 cut(s) 300, 905
BseRI GAGGAG 2 cut(s) 402, 405
BseSI GKGCMC 2 cut(s) 302, 465
BseXI GCAGC 2 cut(s) 124, 194
BshFI GGCC 4 cut(s) 95, 300, 779, 1243
BsiHKAI GWGCWC 4 cut(s) 228, 465, 800, 972
BslI CCNNNNNNNGG 5 cut(s) 617, 840, 841, 881, 979
BsmAI GTCTC 2 cut(s) 320, 1242
BsmBI CGTCTC 1 cut(s) 320
BsnI GGCC 4 cut(s) 95, 300, 779, 1243
Bsp119I TTCGAA 1 cut(s) 689
Bsp120I GGGCCC 1 cut(s) 298
Bsp1286I GDGCHC 5 cut(s) 228, 302, 465, 800, 972
Bsp143I GATC 6 cut(s) 19, 202, 278, 607, 663, 950
BspACI CCGC 1 cut(s) 282
BspANI GGCC 4 cut(s) 95, 300, 779, 1243
BspCNI CTCAG 2 cut(s) 715, 1225
BspLI GGNNCC 3 cut(s) 280, 300, 609
BspPI GGATC 6 cut(s) 273, 286, 602, 615, 658, 958
BspT104I TTCGAA 1 cut(s) 689
BsrI ACTGG 2 cut(s) 300, 905
BssECI CCNNGG 3 cut(s) 380, 780, 1156
BssMI GATC 6 cut(s) 19, 202, 278, 607, 663, 950
BssT1I CCWWGG 1 cut(s) 1156
Bst2UI CCWGG 4 cut(s) 381, 590, 782, 870
Bst4CI ACNGT 5 cut(s) 155, 818, 915, 967, 1277
Bst6I CTCTTC 4 cut(s) 873, 1131, 1143, 1308
BstAPI GCANNNNNTGC 1 cut(s) 851
BstBI TTCGAA 1 cut(s) 689
BstDEI CTNAG 3 cut(s) 723, 838, 1212
BstF5I GGATG 4 cut(s) 248, 427, 733, 901
BstKTI GATC 6 cut(s) 22, 205, 281, 610, 666, 953
BstMAI GTCTC 2 cut(s) 320, 1242
BstMBI GATC 6 cut(s) 19, 202, 278, 607, 663, 950
BstMWI GCNNNNNNNGC 5 cut(s) 121, 179, 306, 851, 1046
BstNI CCWGG 4 cut(s) 381, 590, 782, 870
BstSCI CCNGG 4 cut(s) 379, 588, 780, 868
BstSFI CTRYAG 1 cut(s) 817
BstSLI GKGCMC 2 cut(s) 302, 465
BstV1I GCAGC 2 cut(s) 124, 194
BstV2I GAAGAC 3 cut(s) 12, 51, 678
BstX2I RGATCY 3 cut(s) 278, 607, 663
BstXI CCANNNNNNTGG 1 cut(s) 1013
BstYI RGATCY 3 cut(s) 278, 607, 663
Bsu36I CCTNAGG 1 cut(s) 723
BsuRI GGCC 4 cut(s) 95, 300, 779, 1243
BtrI CACGTC 1 cut(s) 314
BtsCI GGATG 4 cut(s) 248, 427, 733, 901
BtsIMutI CAGTG 1 cut(s) 1273
CaiI CAGNNNCTG 1 cut(s) 875
Cfr13I GGNCC 7 cut(s) 94, 298, 299, 778, 872, 923, 1241
CseI GACGC 1 cut(s) 1205
Csp6I GTAC 2 cut(s) 372, 1058
CviAII CATG 5 cut(s) 32, 193, 253, 530, 1266
CviQI GTAC 2 cut(s) 372, 1058
DdeI CTNAG 3 cut(s) 723, 838, 1212
DpnI GATC 6 cut(s) 21, 204, 280, 609, 665, 952
DpnII GATC 6 cut(s) 19, 202, 278, 607, 663, 950
DrdI GACNNNNNNGTC 1 cut(s) 905
DseDI GACNNNNNNGTC 1 cut(s) 905
Eam1104I CTCTTC 4 cut(s) 873, 1131, 1143, 1308
EarI CTCTTC 4 cut(s) 873, 1131, 1143, 1308
Eco130I CCWWGG 1 cut(s) 1156
Eco24I GRGCYC 1 cut(s) 302
Eco47I GGWCC 2 cut(s) 872, 923
Eco57I CTGAAG 3 cut(s) 350, 897, 1332
Eco81I CCTNAGG 1 cut(s) 723
EcoO109I RGGNCCY 1 cut(s) 872
EcoRI GAATTC 1 cut(s) 746
EcoRII CCWGG 4 cut(s) 379, 588, 780, 868
EcoT14I CCWWGG 1 cut(s) 1156
EcoT38I GRGCYC 1 cut(s) 302
ErhI CCWWGG 1 cut(s) 1156
Esp3I CGTCTC 1 cut(s) 320
FaeI CATG 5 cut(s) 35, 196, 256, 533, 1269
FalI AAGNNNNNCTT 2 cut(s) 1140, 1172
FatI CATG 5 cut(s) 31, 192, 252, 529, 1265
FauNDI CATATG 2 cut(s) 616, 919
Fnu4HI GCNGC 2 cut(s) 113, 183
FokI GGATG 4 cut(s) 235, 434, 740, 908
FriOI GRGCYC 1 cut(s) 302
Fsp4HI GCNGC 2 cut(s) 113, 183
FspBI CTAG 2 cut(s) 138, 510
GluI GCNGC 2 cut(s) 113, 183
GsuI CTGGAG 1 cut(s) 852
HaeIII GGCC 4 cut(s) 95, 300, 779, 1243
HgaI GACGC 1 cut(s) 1205
Hin1II CATG 5 cut(s) 35, 196, 256, 533, 1269
HindIII AAGCTT 1 cut(s) 827
HinfI GANTC 4 cut(s) 35, 262, 399, 683
HphI GGTGA 3 cut(s) 223, 1028, 1044
Hpy166II GTNNAC 3 cut(s) 463, 762, 1271
Hpy188I TCNGA 3 cut(s) 345, 682, 1312
Hpy188III TCNNGA 4 cut(s) 419, 510, 875, 890
Hpy8I GTNNAC 3 cut(s) 463, 762, 1271
HpyAV CCTTC 5 cut(s) 54, 190, 549, 569, 1169
HpyCH4III ACNGT 5 cut(s) 155, 818, 915, 967, 1277
HpyCH4IV ACGT 1 cut(s) 313
HpyCH4V TGCA 5 cut(s) 248, 463, 572, 626, 704
HpyF10VI GCNNNNNNNGC 5 cut(s) 121, 179, 306, 851, 1046
HpyF3I CTNAG 3 cut(s) 723, 838, 1212
HpySE526I ACGT 1 cut(s) 313
Hsp92II CATG 5 cut(s) 35, 196, 256, 533, 1269
Kzo9I GATC 6 cut(s) 19, 202, 278, 607, 663, 950
LmnI GCTCC 2 cut(s) 803, 975
Lsp1109I GCAGC 2 cut(s) 124, 194
LweI GCATC 3 cut(s) 257, 1058, 1081
MaeI CTAG 2 cut(s) 138, 510
MaeII ACGT 1 cut(s) 313
MaeIII GTNAC 2 cut(s) 229, 995
MalI GATC 6 cut(s) 21, 204, 280, 609, 665, 952
MboI GATC 6 cut(s) 19, 202, 278, 607, 663, 950
MflI RGATCY 3 cut(s) 278, 607, 663
MhlI GDGCHC 5 cut(s) 228, 302, 465, 800, 972
MluCI AATT 7 cut(s) 304, 387, 567, 645, 675, 691, 746
MlyI GAGTC 1 cut(s) 692
MmeI TCCRAC 2 cut(s) 691, 723
MroXI GAANNNNTTC 1 cut(s) 39
MseI TTAA 5 cut(s) 351, 557, 824, 860, 977
MspA1I CMGCKG 1 cut(s) 284
MspR9I CCNGG 4 cut(s) 381, 590, 782, 870
MvaI CCWGG 4 cut(s) 381, 590, 782, 870
MwoI GCNNNNNNNGC 5 cut(s) 121, 179, 306, 851, 1046
NdeI CATATG 2 cut(s) 616, 919
NdeII GATC 6 cut(s) 19, 202, 278, 607, 663, 950
NlaIII CATG 5 cut(s) 35, 196, 256, 533, 1269
NlaIV GGNNCC 3 cut(s) 280, 300, 609
NmeAIII GCCGAG 1 cut(s) 1269
NmuCI GTSAC 1 cut(s) 229
NspV TTCGAA 1 cut(s) 689
PcsI WCGNNNNNNNCGW 2 cut(s) 319, 782
PdmI GAANNNNTTC 1 cut(s) 39
PfeI GAWTC 3 cut(s) 35, 262, 399
PflMI CCANNNNNTGG 1 cut(s) 617
PkrI GCNGC 2 cut(s) 114, 184
PleI GAGTC 1 cut(s) 691
PpsI GAGTC 1 cut(s) 691
PpuMI RGGWCCY 1 cut(s) 872
PsiI TTATAA 1 cut(s) 771
Psp5II RGGWCCY 1 cut(s) 872
Psp6I CCWGG 4 cut(s) 379, 588, 780, 868
PspGI CCWGG 4 cut(s) 379, 588, 780, 868
PspN4I GGNNCC 3 cut(s) 280, 300, 609
PspOMI GGGCCC 1 cut(s) 298
PspPI GGNCC 7 cut(s) 94, 298, 299, 778, 872, 923, 1241
PspPPI RGGWCCY 1 cut(s) 872
PstNI CAGNNNCTG 1 cut(s) 875
PsuI RGATCY 3 cut(s) 278, 607, 663
RsaI GTAC 2 cut(s) 373, 1059
RsaNI GTAC 2 cut(s) 372, 1058
SaqAI TTAA 5 cut(s) 351, 557, 824, 860, 977
SatI GCNGC 2 cut(s) 113, 183
Sau3AI GATC 6 cut(s) 19, 202, 278, 607, 663, 950
Sau96I GGNCC 7 cut(s) 94, 298, 299, 778, 872, 923, 1241
SchI GAGTC 1 cut(s) 692
ScrFI CCNGG 4 cut(s) 381, 590, 782, 870
SduI GDGCHC 5 cut(s) 228, 302, 465, 800, 972
SfaNI GCATC 3 cut(s) 257, 1058, 1081
SfcI CTRYAG 1 cut(s) 817
SfuI TTCGAA 1 cut(s) 689
SinI GGWCC 2 cut(s) 872, 923
Sse9I AATT 7 cut(s) 304, 387, 567, 645, 675, 691, 746
SsiI CCGC 1 cut(s) 282
SspMI CTAG 2 cut(s) 138, 510
StyD4I CCNGG 4 cut(s) 379, 588, 780, 868
StyI CCWWGG 1 cut(s) 1156
TaaI ACNGT 5 cut(s) 155, 818, 915, 967, 1277
TaiI ACGT 1 cut(s) 316
TaqI TCGA 3 cut(s) 689, 928, 1184
TasI AATT 7 cut(s) 304, 387, 567, 645, 675, 691, 746
TfiI GAWTC 3 cut(s) 35, 262, 399
Tru1I TTAA 5 cut(s) 351, 557, 824, 860, 977
Tru9I TTAA 5 cut(s) 351, 557, 824, 860, 977
TscAI CASTG 1 cut(s) 1280
TseFI GTSAC 1 cut(s) 229
TseI GCWGC 2 cut(s) 112, 182
Tsp45I GTSAC 1 cut(s) 229
TspDTI ATGAA 8 cut(s) 48, 66, 209, 1098, 1149, 1206, 1358, 1364
TspGWI ACGGA 2 cut(s) 28, 1159
TspRI CASTG 1 cut(s) 1280
Van91I CCANNNNNTGG 1 cut(s) 617
VneI GTGCAC 1 cut(s) 461
VpaK11BI GGWCC 2 cut(s) 872, 923
XapI RAATTY 1 cut(s) 746
XbaI TCTAGA 1 cut(s) 509
XmnI GAANNNNTTC 1 cut(s) 39
XspI CTAG 2 cut(s) 138, 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.