MD11G1073800.v1.1

Domain of unknown function (DUF4792)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0001521
Physical Location & Seq
Reverse (-)
6245019 .. 6248768
3750 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1073800.v1.1.491

Sequence Viewer

Length: 1248 bp
ATGAGAAGTTCTAGGACTTTTACTTATCATGTGAACTTGTGGGTGTCCGATGTAGCAGCGTCTGAAGTGAAGGATGATATTTGGTCGGCCCTTGCTGTGCTTATCACCTTTTGGTTCTTTGCGTCTATGACTCTGATTCTTGGTTTCTATGGATCCGGCAATCTACAACTAGGCCCGAACTGCTCGCGCTTGATACAAACTAATCCATTCTTTGTGCAATCCATTAAGGAGCTTGATGAGCAAAAACCTGGGCCAATGTTATATGGATTCCACAAACCTCCTCCCCTGGATGTTGAAACAGCATGGACCGAAACGAAGGATACGCTTGTTCCAGCCAATTATCACAAGGCATGGATTTACTTTCTAAACAAAGGGTCTAGTATCGATATTATTTACCGTGTAATGCCTACAAGTTCCTCGCCTTTAACCCTTGTAATCGCACAAGGTAGTGAGAGCCTAGCTGAATGGATAGAGGATCCGTCATATGGTAACGCAACATTGTCTTGGAATATAATTTATGCAAGTGGTAGGATCCAGCAGGAAATTCCAAAGTCTTCTACTTACTATATCGCAGTAGGGAACTTGAACCCGGAGAATGTTGAGGTAGAGTTGGTATTCAACATAAAATCTATTCTTTATAACACAACCAAGGCGTATTACAAATGCTCTTTGTATAACCGGTTATGCAGCTTAAAGCTTTCCCTCTTTCGGGCAAATGTTGCTGTCATAACGTCTCCAGGTCCGGAAGAGGGTGTCCCTGACAATGATTGGTATGTAAGCCTGTCTTATGGACCGAGATGGGCCATGTATTTTCTTGTAGCAGGCGTTATGACTTTGCTCCTCTTAACGGCCTTTAAAATTTATTACAAATTCCAAAATTCTAGTGAAGACACAACCGAATTTCAAGCAGAGGAGGAAGAATCCGAGCAAACCCCGTTGCTTCCACCCAAAGACGATGATGTTTTGAGTTGGGGTTCGTCATATGAATCTGTTTCCAATACTGAGGAAGATTTGGATGAGTGCCTTGCAGCGAGTTCCCTTGAAGGAGGGTTAACAAGTGAAGCAGATAGCAACAATACTCGGCGTCTTTGTGTCATTTGCTTTGATGGTCCAAGGGATTGCTTCTTTCTTCCCTGTGGGCACTGCGCTGTTTGTTTTACTTGCGGAACAAGGATTGCTGAGGAGGCTGGTACGTGCCCGATATGCCGTAGAAGGATGAAGAAAGTGAGGAAGATTTTTACAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4792 DUF4793 zf-C3HC4_3

Protein Analysis

416

Amino Acids

46.67

Weight (kDa)

4.88

Isoelectric Point (pI)

48.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APD1-4_N PF16040 84 - 153 2.3e-22 E3 ubiquitin-protein ligase APD1-4, N-terminal
APD1-4_M PF16041 178 - 283 3.7e-33 E3 ubiquitin-protein ligase APD1-4, middle domain
zf-C3HC4_3 PF13920 361 - 409 4.6e-13 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 363 - 403 2e-06 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 639
AccII CGCG 1 cut(s) 187
AccIII TCCGGA 1 cut(s) 742
AciI CCGC 1 cut(s) 1164
AclWI GGATC 6 cut(s) 147, 160, 470, 483, 526, 539
AcsI RAATTY 5 cut(s) 543, 858, 869, 877, 899
AcuI CTGAAG 1 cut(s) 84
AcyI GRCGYC 1 cut(s) 1084
AfaI GTAC 1 cut(s) 1192
AfiI CCNNNNNNNGG 5 cut(s) 485, 708, 709, 749, 847
AgeI ACCGGT 1 cut(s) 678
AgsI TTSAA 5 cut(s) 296, 586, 619, 905, 1043
AjnI CCWGG 3 cut(s) 247, 285, 736
AluBI AGCT 4 cut(s) 232, 461, 690, 697
AluI AGCT 4 cut(s) 232, 461, 690, 697
Alw26I GTCTC 1 cut(s) 738
AlwI GGATC 6 cut(s) 147, 160, 470, 483, 526, 539
AlwNI CAGNNNCTG 1 cut(s) 62
Aor13HI TCCGGA 1 cut(s) 742
AoxI GGCC 5 cut(s) 87, 172, 251, 801, 849
ApeKI GCWGC 3 cut(s) 56, 687, 1028
ApoI RAATTY 5 cut(s) 543, 858, 869, 877, 899
AsiGI ACCGGT 1 cut(s) 678
AspLEI GCGC 2 cut(s) 189, 1148
AspS9I GGNCC 8 cut(s) 88, 173, 251, 306, 740, 791, 801, 1109
AsuC2I CCSGG 1 cut(s) 590
AsuHPI GGTGA 1 cut(s) 97
AvaII GGWCC 4 cut(s) 306, 740, 791, 1109
BaeGI GKGCMC 2 cut(s) 1143, 1199
BaeI ACNNNNGTAYC 2 cut(s) 312, 345
BamHI GGATCC 3 cut(s) 152, 475, 531
BbsI GAAGAC 2 cut(s) 546, 894
BbvCI CCTCAGC 1 cut(s) 1179
BbvI GCAGC 3 cut(s) 68, 699, 1040
BccI CCATC 2 cut(s) 792, 1100
BceAI ACGGC 2 cut(s) 864, 1191
BciT130I CCWGG 3 cut(s) 249, 287, 738
BciVI GTATCC 1 cut(s) 313
BcnI CCSGG 1 cut(s) 590
BcoDI GTCTC 1 cut(s) 738
BfaI CTAG 5 cut(s) 12, 170, 378, 458, 882
BfuI GTATCC 1 cut(s) 313
BisI GCNGC 3 cut(s) 57, 688, 1029
BlsI GCNGC 3 cut(s) 58, 689, 1030
Bme1390I CCNGG 4 cut(s) 249, 287, 590, 738
Bme18I GGWCC 4 cut(s) 306, 740, 791, 1109
BmgT120I GGNCC 8 cut(s) 88, 173, 251, 306, 740, 791, 801, 1109
BmiI GGNNCC 3 cut(s) 154, 477, 533
BmrFI CCNGG 4 cut(s) 249, 287, 590, 738
BpiI GAAGAC 2 cut(s) 546, 894
BpmI CTGGAG 1 cut(s) 720
Bpu10I CCTNAGC 1 cut(s) 1179
BpuMI CCSGG 1 cut(s) 590
Bsa29I ATCGAT 1 cut(s) 384
BsaAI YACGTR 1 cut(s) 1194
BsaHI GRCGYC 1 cut(s) 1084
BsaJI CCNNGG 4 cut(s) 248, 285, 648, 1112
BsaWI WCCGGW 2 cut(s) 678, 742
Bsc4I CCNNNNNNNGG 5 cut(s) 485, 708, 709, 749, 847
Bse118I RCCGGY 1 cut(s) 678
BseAI TCCGGA 1 cut(s) 742
BseBI CCWGG 3 cut(s) 249, 287, 738
BseCI ATCGAT 1 cut(s) 384
BseDI CCNNGG 4 cut(s) 248, 285, 648, 1112
BseGI GGATG 4 cut(s) 79, 295, 1021, 1221
BseLI CCNNNNNNNGG 5 cut(s) 485, 708, 709, 749, 847
BseMII CTCAG 2 cut(s) 993, 1170
BseRI GAGGAG 4 cut(s) 270, 830, 926, 1196
BseSI GKGCMC 2 cut(s) 1143, 1199
BseXI GCAGC 3 cut(s) 68, 699, 1040
Bsh1236I CGCG 1 cut(s) 187
BshFI GGCC 5 cut(s) 89, 174, 253, 803, 851
BshTI ACCGGT 1 cut(s) 678
BshVI ATCGAT 1 cut(s) 384
BsiSI CCGG 4 cut(s) 156, 590, 679, 743
BslFI GGGAC 1 cut(s) 740
BslI CCNNNNNNNGG 5 cut(s) 485, 708, 709, 749, 847
BsmAI GTCTC 1 cut(s) 738
BsmBI CGTCTC 1 cut(s) 738
BsmFI GGGAC 1 cut(s) 740
BsnI GGCC 5 cut(s) 89, 174, 253, 803, 851
Bsp1286I GDGCHC 2 cut(s) 1143, 1199
Bsp13I TCCGGA 1 cut(s) 742
Bsp143I GATC 3 cut(s) 152, 475, 531
BspACI CCGC 1 cut(s) 1164
BspANI GGCC 5 cut(s) 89, 174, 253, 803, 851
BspCNI CTCAG 2 cut(s) 994, 1171
BspDI ATCGAT 1 cut(s) 384
BspEI TCCGGA 1 cut(s) 742
BspFNI CGCG 1 cut(s) 187
BspLI GGNNCC 3 cut(s) 154, 477, 533
BspPI GGATC 6 cut(s) 147, 160, 470, 483, 526, 539
BsrFI RCCGGY 1 cut(s) 678
BssAI RCCGGY 1 cut(s) 678
BssECI CCNNGG 4 cut(s) 248, 285, 648, 1112
BssMI GATC 3 cut(s) 152, 475, 531
BssNI GRCGYC 1 cut(s) 1084
BssT1I CCWWGG 2 cut(s) 648, 1112
Bst2UI CCWGG 3 cut(s) 249, 287, 738
Bst4CI ACNGT 2 cut(s) 398, 1243
Bst6I CTCTTC 1 cut(s) 741
BstACI GRCGYC 1 cut(s) 1084
BstAPI GCANNNNNTGC 1 cut(s) 719
BstBAI YACGTR 1 cut(s) 1194
BstC8I GCNNGC 2 cut(s) 185, 823
BstDEI CTNAG 2 cut(s) 1002, 1179
BstF5I GGATG 4 cut(s) 79, 295, 1021, 1221
BstFNI CGCG 1 cut(s) 187
BstHHI GCGC 2 cut(s) 189, 1148
BstKTI GATC 3 cut(s) 155, 478, 534
BstMAI GTCTC 1 cut(s) 738
BstMBI GATC 3 cut(s) 152, 475, 531
BstMWI GCNNNNNNNGC 5 cut(s) 180, 238, 719, 1184, 1203
BstNI CCWGG 3 cut(s) 249, 287, 738
BstSCI CCNGG 4 cut(s) 247, 285, 588, 736
BstSLI GKGCMC 2 cut(s) 1143, 1199
BstUI CGCG 1 cut(s) 187
BstV1I GCAGC 3 cut(s) 68, 699, 1040
BstV2I GAAGAC 2 cut(s) 546, 894
BstX2I RGATCY 3 cut(s) 152, 475, 531
BstYI RGATCY 3 cut(s) 152, 475, 531
Bsu15I ATCGAT 1 cut(s) 384
BsuI GTATCC 1 cut(s) 313
BsuRI GGCC 5 cut(s) 89, 174, 253, 803, 851
BsuTUI ATCGAT 1 cut(s) 384
BtsCI GGATG 4 cut(s) 79, 295, 1021, 1221
BtsI GCAGTG 1 cut(s) 1141
BtsIMutI CAGTG 1 cut(s) 1141
Cac8I GCNNGC 2 cut(s) 185, 823
CaiI CAGNNNCTG 1 cut(s) 62
CfoI GCGC 2 cut(s) 189, 1148
Cfr10I RCCGGY 1 cut(s) 678
Cfr13I GGNCC 8 cut(s) 88, 173, 251, 306, 740, 791, 801, 1109
ClaI ATCGAT 1 cut(s) 384
CseI GACGC 3 cut(s) 48, 111, 1073
Csp6I GTAC 1 cut(s) 1191
CspAI ACCGGT 1 cut(s) 678
CviAII CATG 4 cut(s) 29, 303, 351, 805
CviQI GTAC 1 cut(s) 1191
DdeI CTNAG 2 cut(s) 1002, 1179
DpnI GATC 3 cut(s) 154, 477, 533
DpnII GATC 3 cut(s) 152, 475, 531
DraI TTTAAA 1 cut(s) 856
Eam1104I CTCTTC 1 cut(s) 741
EarI CTCTTC 1 cut(s) 741
Eco130I CCWWGG 2 cut(s) 648, 1112
Eco47I GGWCC 4 cut(s) 306, 740, 791, 1109
Eco57I CTGAAG 1 cut(s) 84
EcoRII CCWGG 3 cut(s) 247, 285, 736
EcoT14I CCWWGG 2 cut(s) 648, 1112
ErhI CCWWGG 2 cut(s) 648, 1112
Esp3I CGTCTC 1 cut(s) 738
FaeI CATG 4 cut(s) 32, 306, 354, 808
FalI AAGNNNNNCTT 2 cut(s) 769, 801
FaqI GGGAC 1 cut(s) 740
FatI CATG 4 cut(s) 28, 302, 350, 804
FauNDI CATATG 2 cut(s) 484, 982
Fnu4HI GCNGC 3 cut(s) 57, 688, 1029
FokI GGATG 4 cut(s) 86, 302, 1028, 1228
Fsp4HI GCNGC 3 cut(s) 57, 688, 1029
FspBI CTAG 5 cut(s) 12, 170, 378, 458, 882
GlaI GCGC 2 cut(s) 188, 1147
GluI GCNGC 3 cut(s) 57, 688, 1029
GsuI CTGGAG 1 cut(s) 720
HaeIII GGCC 5 cut(s) 89, 174, 253, 803, 851
HapII CCGG 4 cut(s) 156, 590, 679, 743
HgaI GACGC 3 cut(s) 48, 111, 1073
HhaI GCGC 2 cut(s) 189, 1148
Hin1I GRCGYC 1 cut(s) 1084
Hin1II CATG 4 cut(s) 32, 306, 354, 808
Hin6I GCGC 2 cut(s) 187, 1146
HinP1I GCGC 2 cut(s) 187, 1146
HincII GTYRAC 1 cut(s) 1053
HindII GTYRAC 1 cut(s) 1053
HindIII AAGCTT 1 cut(s) 695
HinfI GANTC 5 cut(s) 130, 136, 267, 920, 986
HpaI GTTAAC 1 cut(s) 1053
HpaII CCGG 4 cut(s) 156, 590, 679, 743
HphI GGTGA 1 cut(s) 97
Hpy166II GTNNAC 2 cut(s) 34, 1053
Hpy188I TCNGA 4 cut(s) 49, 64, 135, 925
Hpy188III TCNNGA 1 cut(s) 743
Hpy8I GTNNAC 2 cut(s) 34, 1053
HpyAV CCTTC 4 cut(s) 64, 310, 1037, 1206
HpyCH4III ACNGT 2 cut(s) 398, 1243
HpyCH4IV ACGT 2 cut(s) 731, 1193
HpyCH4V TGCA 4 cut(s) 217, 521, 687, 1028
HpyF10VI GCNNNNNNNGC 5 cut(s) 180, 238, 719, 1184, 1203
HpyF3I CTNAG 2 cut(s) 1002, 1179
HpySE526I ACGT 2 cut(s) 731, 1193
Hsp92I GRCGYC 1 cut(s) 1084
Hsp92II CATG 4 cut(s) 32, 306, 354, 808
HspAI GCGC 2 cut(s) 187, 1146
Kpn2I TCCGGA 1 cut(s) 742
KspAI GTTAAC 1 cut(s) 1053
Kzo9I GATC 3 cut(s) 152, 475, 531
LmnI GCTCC 2 cut(s) 229, 843
Lsp1109I GCAGC 3 cut(s) 68, 699, 1040
MaeI CTAG 5 cut(s) 12, 170, 378, 458, 882
MaeII ACGT 2 cut(s) 731, 1193
MaeIII GTNAC 1 cut(s) 488
MalI GATC 3 cut(s) 154, 477, 533
MboI GATC 3 cut(s) 152, 475, 531
MboII GAAGA 8 cut(s) 546, 758, 899, 929, 1019, 1121, 1231, 1243
MflI RGATCY 3 cut(s) 152, 475, 531
MhlI GDGCHC 2 cut(s) 1143, 1199
MluCI AATT 7 cut(s) 337, 513, 543, 858, 869, 877, 899
MlyI GAGTC 1 cut(s) 124
MroI TCCGGA 1 cut(s) 742
MseI TTAA 6 cut(s) 225, 425, 692, 845, 855, 1052
MspI CCGG 4 cut(s) 156, 590, 679, 743
MspR9I CCNGG 4 cut(s) 249, 287, 590, 738
MvaI CCWGG 3 cut(s) 249, 287, 738
MvnI CGCG 1 cut(s) 187
MwoI GCNNNNNNNGC 5 cut(s) 180, 238, 719, 1184, 1203
NciI CCSGG 1 cut(s) 590
NdeI CATATG 2 cut(s) 484, 982
NdeII GATC 3 cut(s) 152, 475, 531
NlaIII CATG 4 cut(s) 32, 306, 354, 808
NlaIV GGNNCC 3 cut(s) 154, 477, 533
NmeAIII GCCGAG 1 cut(s) 1060
PfeI GAWTC 4 cut(s) 136, 267, 920, 986
PinAI ACCGGT 1 cut(s) 678
PkrI GCNGC 3 cut(s) 58, 689, 1030
PleI GAGTC 1 cut(s) 124
PpsI GAGTC 1 cut(s) 124
Ppu21I YACGTR 1 cut(s) 1194
PsiI TTATAA 1 cut(s) 639
Psp6I CCWGG 3 cut(s) 247, 285, 736
PspGI CCWGG 3 cut(s) 247, 285, 736
PspN4I GGNNCC 3 cut(s) 154, 477, 533
PspPI GGNCC 8 cut(s) 88, 173, 251, 306, 740, 791, 801, 1109
PstNI CAGNNNCTG 1 cut(s) 62
PsuI RGATCY 3 cut(s) 152, 475, 531
RsaI GTAC 1 cut(s) 1192
RsaNI GTAC 1 cut(s) 1191
SaqAI TTAA 6 cut(s) 225, 425, 692, 845, 855, 1052
SatI GCNGC 3 cut(s) 57, 688, 1029
Sau3AI GATC 3 cut(s) 152, 475, 531
Sau96I GGNCC 8 cut(s) 88, 173, 251, 306, 740, 791, 801, 1109
SchI GAGTC 1 cut(s) 124
ScrFI CCNGG 4 cut(s) 249, 287, 590, 738
SduI GDGCHC 2 cut(s) 1143, 1199
SinI GGWCC 4 cut(s) 306, 740, 791, 1109
Sse9I AATT 7 cut(s) 337, 513, 543, 858, 869, 877, 899
SsiI CCGC 1 cut(s) 1164
SspMI CTAG 5 cut(s) 12, 170, 378, 458, 882
StyD4I CCNGG 4 cut(s) 247, 285, 588, 736
StyI CCWWGG 2 cut(s) 648, 1112
TaaI ACNGT 2 cut(s) 398, 1243
TaiI ACGT 2 cut(s) 734, 1196
TaqI TCGA 1 cut(s) 384
TaqII GACCGA 2 cut(s) 323, 808
TasI AATT 7 cut(s) 337, 513, 543, 858, 869, 877, 899
TfiI GAWTC 4 cut(s) 136, 267, 920, 986
Tru1I TTAA 6 cut(s) 225, 425, 692, 845, 855, 1052
Tru9I TTAA 6 cut(s) 225, 425, 692, 845, 855, 1052
TscAI CASTG 1 cut(s) 1148
TseI GCWGC 3 cut(s) 56, 687, 1028
TspDTI ATGAA 2 cut(s) 999, 1232
TspGWI ACGGA 1 cut(s) 468
TspRI CASTG 1 cut(s) 1148
VpaK11BI GGWCC 4 cut(s) 306, 740, 791, 1109
XapI RAATTY 5 cut(s) 543, 858, 869, 877, 899
XspI CTAG 5 cut(s) 12, 170, 378, 458, 882
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.