Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001521

Physical Location & Seq

Genomic Coordinates Forward (+)

24716164 .. 24720305

Gene Structure 4142 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1166600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1164 bp

ATGACTCGTGGTCCTGCATCGTCGTCCTCCTCACCTTCTGGTTCTTCGGTTTTGTCGATGACTTTGATTTTGGGAGTTTACGGGTCTTCGATTTTAGAGCTTGGCCCTAATAGCTCAATTCTTCTGCAACCTAACCCATTTTTTGTGCAGTCTATAAAGGTAGAAGAGCTGAATATATCAAGTCCTGGGCCTGTACTATATGGTTTCTACAGTACTCCACCTCTTGATACTGCTACCACTTGGTCCGAAATGCATACTGTCTCGGTTCCAGCTGATTCTCATACGGAATGGATATATTACCTAAATGCGGGGTCTCAAATGAGTATTTCATACAGCGTGAACTCTCCGAGCTTCTCTATTTTTCTTATTATTGCTCAAGGTAAAGAAGGCCTTGCTCAGTGGCTGGAAGAACCGACATATCCTAATACTTCTTTATCATGGAACCTCATTCATGGAAGTGGTATGATTGAGCAGGACCTATCAAAGTCTTCTACCTACTATGTTTCTGTGGGTAACTTGAATGCAGAGGATGTGGAGGTACAATTAAACATCACAGTGAATGCTTTATTGTACAATACAAATTCGGCTTATTACAAATGTACTTTCACGGATGAATCCTGTACTTTGAAAATTTTGTTTCCGGAGGGAAATGAAGCTGTGTTGACCACTCCTGGTCCAGAGCAGGATACACCTTCTGCAAACCAGTATGTCAAACTATCATATGGACCAAGATGGGCCACATATATTGTTGGTATAGGTGGGATGACTGTGCTCATGTTCTTGGCCTTCAACTTCTTAAACAAGTGCAATGAAGATGGAATAAGAGTACGACATGATGAGTTCAGATCTGAGAGAGCCCCATTGCTTTCCTACAAAGATGATGATCAATCAAGTTGGGGCTCATCTTATGATTCTGCCTCAAACGATGAGGGGGATCTTGAAGACTTACTGGCAGGAGAAGGGAAGATGTCAAGAGATGGTGAAAGTAATAATACTCGTCGTCTTTGCGCAATCTGCTTTGATGCTCCTAGGGACTGCTTTTTCCTTCCCTGCGGGCATTGTGTGGCTTGTTTTGAGTGTGCAACAAGGATAGCAGAGGCAGCTGCTACTTGTCCAATTTGTCGGAGGAACATAAAGAAGGTGAGGAAGATCTTTACAGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0031625 GO:0032446 GO:0032991 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044389 GO:0044424 GO:0044464 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF4792 DUF4793 zf-C3HC4_3

Protein Analysis

388

Amino Acids

42.49

Weight (kDa)

4.74

Isoelectric Point (pI)

56.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
APD1-4_N	PF16040	63 - 131	3.3e-23	E3 ubiquitin-protein ligase APD1-4, N-terminal
APD1-4_M	PF16041	156 - 261	2.8e-32	E3 ubiquitin-protein ligase APD1-4, middle domain
zf-C3HC4_3	PF13920	333 - 381	3.5e-14	Zinc finger, C3HC4 type (RING finger)
zf-RING_2	PF13639	335 - 375	1.8e-06	Ring finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001521)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01450
fragaria_vesca	FvH4_3g38600 FvH4_3g38600 FvH4_3g38600 FvH4_3g38600 FvH4_3g38600 FvH4_3g38600 FvH4_3g38600 FvH4_3g38600 FvH4_3g38600 FvH4_3g38600 FvH4_6g11430 FvH4_6g11430 FvH4_6g11430 FvH4_6g11430 FvH4_6g11430
malus_domestica	MD03G1070000.v1.1 MD04G1153100.v1.1 MD11G1073800.v1.1 MD12G1166600.v1.1
prunus_persica	Prupe.6G057100_v2.0.a1 Prupe.6G057100_v2.0.a1 Prupe.6G277400_v2.0.a1 Prupe.6G277400_v2.0.a1
pyrus_communis	pycom03g05570 pycom11g06120 pycom12g15850
rosa_chinensis	RchiOBHm_Chr3g0463121 RchiOBHm_Chr5g0069661
rosa_laevigata	RLG00000024811 RLG00000036082
rosa_multiflora	Rmu_sc0000738.1_g000037 Rmu_sc0000766.1_g000040 Rmu_sc0008595.1_g000001
rosa_roxburghii	Rroxscaffold_1G00011420 Rroxscaffold_6G00416950
rosa_rugosa	Rorug03G0061500 Rorug03G0061600 Rorug03G0061700 Rorug05G0399000 Rorug05G0399100
rosa_samantha	Rh3AG119800 Rh3BG124200 Rh3CG126200 Rh3DG125200 Rh5AG455800 Rh5BG474800 Rh5CG498200 Rh5DG486300
rosa_wichuraiana	Rw3G010130 Rw5G042530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1009
AccIII	TCCGGA	1 cut(s)	640
AciI	CCGC	2 cut(s)	308, 1053
AclWI	GGATC	1 cut(s)	942
AcsI	RAATTY	2 cut(s)	580, 630
AfaI	GTAC	7 cut(s)	195, 214, 540, 572, 601, 622, 828
AfiI	CCNNNNNNNGG	1 cut(s)	307
AgsI	TTSAA	4 cut(s)	520, 628, 790, 941
AhdI	GACNNNNNGTC	1 cut(s)	9
AjnI	CCWGG	2 cut(s)	184, 670
AluBI	AGCT	7 cut(s)	100, 114, 169, 272, 351, 656, 1103
AluI	AGCT	7 cut(s)	100, 114, 169, 272, 351, 656, 1103
Alw21I	GWGCWC	1 cut(s)	774
Alw26I	GTCTC	2 cut(s)	265, 318
AlwI	GGATC	1 cut(s)	942
AlwNI	CAGNNNCTG	1 cut(s)	403
Aor13HI	TCCGGA	1 cut(s)	640
AoxI	GGCC	5 cut(s)	103, 188, 388, 735, 783
ApeKI	GCWGC	2 cut(s)	1100, 1103
ApoI	RAATTY	2 cut(s)	580, 630
ArsI	GACNNNNNNTTYG	2 cut(s)	52, 84
AspA2I	CCTAGG	1 cut(s)	1028
AspLEI	GCGC	1 cut(s)	1010
AspS9I	GGNCC	8 cut(s)	11, 104, 188, 243, 475, 674, 725, 735
AsuHPI	GGTGA	3 cut(s)	24, 992, 1153
AvaII	GGWCC	5 cut(s)	11, 243, 475, 674, 725
AvrII	CCTAGG	1 cut(s)	1028
BanII	GRGCYC	2 cut(s)	859, 902
BauI	CACGAG	1 cut(s)	6
BbsI	GAAGAC	3 cut(s)	78, 480, 948
Bbv12I	GWGCWC	1 cut(s)	774
BbvI	GCAGC	2 cut(s)	1090, 1112
BccI	CCATC	3 cut(s)	726, 809, 971
BciT130I	CCWGG	2 cut(s)	186, 672
BciVI	GTATCC	1 cut(s)	679
BclI	TGATCA	1 cut(s)	883
BcoDI	GTCTC	2 cut(s)	265, 318
BfaI	CTAG	1 cut(s)	1029
BfmI	CTRYAG	1 cut(s)	208
BfuI	GTATCC	1 cut(s)	679
BglII	AGATCT	2 cut(s)	845, 1149
BisI	GCNGC	2 cut(s)	1101, 1104
BlnI	CCTAGG	1 cut(s)	1028
BlsI	GCNGC	2 cut(s)	1102, 1105
BmcAI	AGTACT	1 cut(s)	214
Bme1390I	CCNGG	2 cut(s)	186, 672
Bme18I	GGWCC	5 cut(s)	11, 243, 475, 674, 725
BmeRI	GACNNNNNGTC	1 cut(s)	9
BmgT120I	GGNCC	8 cut(s)	11, 104, 188, 243, 475, 674, 725, 735
BmiI	GGNNCC	2 cut(s)	267, 443
BmrFI	CCNGG	2 cut(s)	186, 672
BmsI	GCATC	2 cut(s)	26, 1012
BpiI	GAAGAC	3 cut(s)	78, 480, 948
BpuEI	CTTGAG	1 cut(s)	360
BsaBI	GATNNNNATC	1 cut(s)	882
BsaI	GGTCTC	1 cut(s)	318
BsaJI	CCNNGG	2 cut(s)	185, 1028
BsaWI	WCCGGW	1 cut(s)	640
Bsc4I	CCNNNNNNNGG	1 cut(s)	307
Bse1I	ACTGG	2 cut(s)	703, 954
Bse3DI	GCAATG	2 cut(s)	814, 860
Bse8I	GATNNNNATC	1 cut(s)	882
BseAI	TCCGGA	1 cut(s)	640
BseBI	CCWGG	2 cut(s)	186, 672
BseDI	CCNNGG	2 cut(s)	185, 1028
BseGI	GGATG	3 cut(s)	535, 616, 768
BseJI	GATNNNNATC	1 cut(s)	882
BseLI	CCNNNNNNNGG	1 cut(s)	307
BseMI	GCAATG	2 cut(s)	814, 860
BseMII	CTCAG	2 cut(s)	410, 840
BseNI	ACTGG	2 cut(s)	703, 954
BseRI	GAGGAG	1 cut(s)	19
BseXI	GCAGC	2 cut(s)	1090, 1112
BsgI	GTGCAG	1 cut(s)	167
BshFI	GGCC	5 cut(s)	105, 190, 390, 737, 785
BsiHKAI	GWGCWC	1 cut(s)	774
BsiSI	CCGG	1 cut(s)	641
BslFI	GGGAC	1 cut(s)	1046
BslI	CCNNNNNNNGG	1 cut(s)	307
BsmAI	GTCTC	2 cut(s)	265, 318
BsmFI	GGGAC	1 cut(s)	1046
BsmI	GAATGC	2 cut(s)	526, 565
BsnI	GGCC	5 cut(s)	105, 190, 390, 737, 785
Bso31I	GGTCTC	1 cut(s)	318
Bsp1286I	GDGCHC	3 cut(s)	774, 859, 902
Bsp13I	TCCGGA	1 cut(s)	640
Bsp1407I	TGTACA	1 cut(s)	570
Bsp143I	GATC	4 cut(s)	845, 883, 934, 1149
BspACI	CCGC	2 cut(s)	308, 1053
BspANI	GGCC	5 cut(s)	105, 190, 390, 737, 785
BspCNI	CTCAG	2 cut(s)	409, 841
BspEI	TCCGGA	1 cut(s)	640
BspLI	GGNNCC	2 cut(s)	267, 443
BspPI	GGATC	1 cut(s)	942
BspQI	GCTCTTC	1 cut(s)	159
BspTNI	GGTCTC	1 cut(s)	318
BsrDI	GCAATG	2 cut(s)	814, 860
BsrGI	TGTACA	1 cut(s)	570
BsrI	ACTGG	2 cut(s)	703, 954
BssECI	CCNNGG	2 cut(s)	185, 1028
BssMI	GATC	4 cut(s)	845, 883, 934, 1149
BssSI	CACGAG	1 cut(s)	6
BssT1I	CCWWGG	1 cut(s)	1028
Bst2BI	CACGAG	1 cut(s)	6
Bst2UI	CCWGG	2 cut(s)	186, 672
Bst4CI	ACNGT	5 cut(s)	212, 259, 556, 769, 1159
Bst6I	CTCTTC	1 cut(s)	159
BstAUI	TGTACA	1 cut(s)	570
BstC8I	GCNNGC	1 cut(s)	1055
BstDEI	CTNAG	2 cut(s)	396, 849
BstF5I	GGATG	3 cut(s)	535, 616, 768
BstHHI	GCGC	1 cut(s)	1010
BstKTI	GATC	4 cut(s)	848, 886, 937, 1152
BstMAI	GTCTC	2 cut(s)	265, 318
BstMBI	GATC	4 cut(s)	845, 883, 934, 1149
BstMWI	GCNNNNNNNGC	3 cut(s)	111, 1014, 1100
BstNI	CCWGG	2 cut(s)	186, 672
BstSCI	CCNGG	2 cut(s)	184, 670
BstSFI	CTRYAG	1 cut(s)	208
BstV1I	GCAGC	2 cut(s)	1090, 1112
BstV2I	GAAGAC	3 cut(s)	78, 480, 948
BstX2I	RGATCY	3 cut(s)	845, 934, 1149
BstYI	RGATCY	3 cut(s)	845, 934, 1149
BsuI	GTATCC	1 cut(s)	679
BsuRI	GGCC	5 cut(s)	105, 190, 390, 737, 785
BtsCI	GGATG	3 cut(s)	535, 616, 768
BtsIMutI	CAGTG	2 cut(s)	404, 561
Cac8I	GCNNGC	1 cut(s)	1055
CaiI	CAGNNNCTG	1 cut(s)	403
CfoI	GCGC	1 cut(s)	1010
Cfr13I	GGNCC	8 cut(s)	11, 104, 188, 243, 475, 674, 725, 735
Csp6I	GTAC	7 cut(s)	194, 213, 539, 571, 600, 621, 827
CspCI	CAANNNNNGTGG	2 cut(s)	727, 762
CviAII	CATG	4 cut(s)	438, 452, 775, 833
CviQI	GTAC	7 cut(s)	194, 213, 539, 571, 600, 621, 827
DdeI	CTNAG	2 cut(s)	396, 849
DpnI	GATC	4 cut(s)	847, 885, 936, 1151
DpnII	GATC	4 cut(s)	845, 883, 934, 1149
DriI	GACNNNNNGTC	1 cut(s)	9
Eam1104I	CTCTTC	1 cut(s)	159
Eam1105I	GACNNNNNGTC	1 cut(s)	9
EarI	CTCTTC	1 cut(s)	159
Eco130I	CCWWGG	1 cut(s)	1028
Eco147I	AGGCCT	1 cut(s)	390
Eco24I	GRGCYC	2 cut(s)	859, 902
Eco31I	GGTCTC	1 cut(s)	318
Eco47I	GGWCC	5 cut(s)	11, 243, 475, 674, 725
EcoO109I	RGGNCCY	1 cut(s)	475
EcoRII	CCWGG	2 cut(s)	184, 670
EcoT14I	CCWWGG	1 cut(s)	1028
EcoT22I	ATGCAT	1 cut(s)	255
EcoT38I	GRGCYC	2 cut(s)	859, 902
ErhI	CCWWGG	1 cut(s)	1028
FaeI	CATG	4 cut(s)	441, 455, 778, 836
FalI	AAGNNNNNCTT	2 cut(s)	375, 407
FaqI	GGGAC	1 cut(s)	1046
FatI	CATG	4 cut(s)	437, 451, 774, 832
FauI	CCCGC	2 cut(s)	301, 1046
FauNDI	CATATG	1 cut(s)	721
FbaI	TGATCA	1 cut(s)	883
Fnu4HI	GCNGC	2 cut(s)	1101, 1104
FokI	GGATG	3 cut(s)	542, 623, 775
FriOI	GRGCYC	2 cut(s)	859, 902
Fsp4HI	GCNGC	2 cut(s)	1101, 1104
FspBI	CTAG	1 cut(s)	1029
FspI	TGCGCA	1 cut(s)	1009
GlaI	GCGC	1 cut(s)	1009
GluI	GCNGC	2 cut(s)	1101, 1104
HaeIII	GGCC	5 cut(s)	105, 190, 390, 737, 785
HapII	CCGG	1 cut(s)	641
HhaI	GCGC	1 cut(s)	1010
Hin1II	CATG	4 cut(s)	441, 455, 778, 836
Hin6I	GCGC	1 cut(s)	1008
HinP1I	GCGC	1 cut(s)	1008
HincII	GTYRAC	1 cut(s)	663
HindII	GTYRAC	1 cut(s)	663
HinfI	GANTC	4 cut(s)	4, 275, 614, 911
HpaII	CCGG	1 cut(s)	641
HphI	GGTGA	3 cut(s)	24, 992, 1153
Hpy166II	GTNNAC	3 cut(s)	79, 340, 663
Hpy188I	TCNGA	5 cut(s)	247, 348, 845, 850, 1125
Hpy188III	TCNNGA	5 cut(s)	224, 641, 677, 938, 972
Hpy8I	GTNNAC	3 cut(s)	79, 340, 663
Hpy99I	CGWCG	2 cut(s)	25, 1002
HpyAV	CCTTC	7 cut(s)	45, 380, 702, 796, 953, 1055, 1132
HpyCH4III	ACNGT	5 cut(s)	212, 259, 556, 769, 1159
HpyCH4V	TGCA	8 cut(s)	17, 127, 148, 253, 524, 698, 807, 1082
HpyF10VI	GCNNNNNNNGC	3 cut(s)	111, 1014, 1100
HpyF3I	CTNAG	2 cut(s)	396, 849
Hsp92II	CATG	4 cut(s)	441, 455, 778, 836
HspAI	GCGC	1 cut(s)	1008
Kpn2I	TCCGGA	1 cut(s)	640
Ksp22I	TGATCA	1 cut(s)	883
Kzo9I	GATC	4 cut(s)	845, 883, 934, 1149
LguI	GCTCTTC	1 cut(s)	159
LmnI	GCTCC	1 cut(s)	1030
Lsp1109I	GCAGC	2 cut(s)	1090, 1112
LweI	GCATC	2 cut(s)	26, 1012
MaeI	CTAG	1 cut(s)	1029
MaeIII	GTNAC	1 cut(s)	512
MalI	GATC	4 cut(s)	847, 885, 936, 1151
MboI	GATC	4 cut(s)	845, 883, 934, 1149
MflI	RGATCY	3 cut(s)	845, 934, 1149
MhlI	GDGCHC	3 cut(s)	774, 859, 902
MluCI	AATT	5 cut(s)	117, 542, 580, 630, 1116
MmeI	TCCRAC	1 cut(s)	1103
Mph1103I	ATGCAT	1 cut(s)	255
MroI	TCCGGA	1 cut(s)	640
MseI	TTAA	3 cut(s)	545, 797, 1162
MslI	CAYNNNNRTG	2 cut(s)	456, 554
MspA1I	CMGCKG	2 cut(s)	272, 1103
MspI	CCGG	1 cut(s)	641
MspR9I	CCNGG	2 cut(s)	186, 672
Mva1269I	GAATGC	2 cut(s)	526, 565
MvaI	CCWGG	2 cut(s)	186, 672
MwoI	GCNNNNNNNGC	3 cut(s)	111, 1014, 1100
NdeI	CATATG	1 cut(s)	721
NdeII	GATC	4 cut(s)	845, 883, 934, 1149
NlaIII	CATG	4 cut(s)	441, 455, 778, 836
NlaIV	GGNNCC	2 cut(s)	267, 443
NsbI	TGCGCA	1 cut(s)	1009
NsiI	ATGCAT	1 cut(s)	255
PceI	AGGCCT	1 cut(s)	390
PciSI	GCTCTTC	1 cut(s)	159
PcsI	WCGNNNNNNNCGW	1 cut(s)	53
PctI	GAATGC	2 cut(s)	526, 565
PfeI	GAWTC	3 cut(s)	275, 614, 911
PkrI	GCNGC	2 cut(s)	1102, 1105
PpuMI	RGGWCCY	1 cut(s)	475
Psp5II	RGGWCCY	1 cut(s)	475
Psp6I	CCWGG	2 cut(s)	184, 670
PspGI	CCWGG	2 cut(s)	184, 670
PspN4I	GGNNCC	2 cut(s)	267, 443
PspPI	GGNCC	8 cut(s)	11, 104, 188, 243, 475, 674, 725, 735
PspPPI	RGGWCCY	1 cut(s)	475
PstNI	CAGNNNCTG	1 cut(s)	403
PsuI	RGATCY	3 cut(s)	845, 934, 1149
PvuII	CAGCTG	2 cut(s)	272, 1103
RsaI	GTAC	7 cut(s)	195, 214, 540, 572, 601, 622, 828
RsaNI	GTAC	7 cut(s)	194, 213, 539, 571, 600, 621, 827
RseI	CAYNNNNRTG	2 cut(s)	456, 554
SapI	GCTCTTC	1 cut(s)	159
SaqAI	TTAA	3 cut(s)	545, 797, 1162
SatI	GCNGC	2 cut(s)	1101, 1104
Sau3AI	GATC	4 cut(s)	845, 883, 934, 1149
Sau96I	GGNCC	8 cut(s)	11, 104, 188, 243, 475, 674, 725, 735
ScaI	AGTACT	1 cut(s)	214
ScrFI	CCNGG	2 cut(s)	186, 672
SduI	GDGCHC	3 cut(s)	774, 859, 902
SfaNI	GCATC	2 cut(s)	26, 1012
SfcI	CTRYAG	1 cut(s)	208
SinI	GGWCC	5 cut(s)	11, 243, 475, 674, 725
SmiMI	CAYNNNNRTG	2 cut(s)	456, 554
SmlI	CTYRAG	1 cut(s)	375
SmoI	CTYRAG	1 cut(s)	375
Sse9I	AATT	5 cut(s)	117, 542, 580, 630, 1116
SseBI	AGGCCT	1 cut(s)	390
SsiI	CCGC	2 cut(s)	308, 1053
SspMI	CTAG	1 cut(s)	1029
StuI	AGGCCT	1 cut(s)	390
StyD4I	CCNGG	2 cut(s)	184, 670
StyI	CCWWGG	1 cut(s)	1028
TaaI	ACNGT	5 cut(s)	212, 259, 556, 769, 1159
TaqI	TCGA	2 cut(s)	56, 89
TasI	AATT	5 cut(s)	117, 542, 580, 630, 1116
TatI	WGTACW	5 cut(s)	193, 212, 570, 599, 620
TfiI	GAWTC	3 cut(s)	275, 614, 911
Tru1I	TTAA	3 cut(s)	545, 797, 1162
Tru9I	TTAA	3 cut(s)	545, 797, 1162
TscAI	CASTG	2 cut(s)	404, 561
TseI	GCWGC	2 cut(s)	1100, 1103
TspDTI	ATGAA	5 cut(s)	318, 440, 627, 666, 825
TspGWI	ACGGA	2 cut(s)	299, 623
TspRI	CASTG	2 cut(s)	404, 561
VpaK11BI	GGWCC	5 cut(s)	11, 243, 475, 674, 725
XapI	RAATTY	2 cut(s)	580, 630
XmaJI	CCTAGG	1 cut(s)	1028
XspI	CTAG	1 cut(s)	1029
ZrmI	AGTACT	1 cut(s)	214
Zsp2I	ATGCAT	1 cut(s)	255

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.